JP5004586B2 - ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し - Google Patents

ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し Download PDF

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JP5004586B2
JP5004586B2 JP2006538022A JP2006538022A JP5004586B2 JP 5004586 B2 JP5004586 B2 JP 5004586B2 JP 2006538022 A JP2006538022 A JP 2006538022A JP 2006538022 A JP2006538022 A JP 2006538022A JP 5004586 B2 JP5004586 B2 JP 5004586B2
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antibody
buffer
resin
hydroxyapatite
protein
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JP2007532477A (ja
JP2007532477A5 (https=
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シュジュン・スン
クリストファー・ガロ
ブライアン・ケリー
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ワイス・エルエルシー
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2006538022A 2003-10-27 2004-10-06 ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し Expired - Lifetime JP5004586B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US51401803P 2003-10-27 2003-10-27
US60/514,018 2003-10-27
US52333503P 2003-11-20 2003-11-20
US60/523,335 2003-11-20
PCT/US2004/032883 WO2005044856A2 (en) 2003-10-27 2004-10-06 Removal of high molecular weight aggregates using hydroxyapatite chromatography

Related Child Applications (1)

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JP2012013648A Division JP5490156B2 (ja) 2003-10-27 2012-01-26 ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し

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JP2007532477A JP2007532477A (ja) 2007-11-15
JP2007532477A5 JP2007532477A5 (https=) 2012-03-22
JP5004586B2 true JP5004586B2 (ja) 2012-08-22

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JP2012013648A Expired - Lifetime JP5490156B2 (ja) 2003-10-27 2012-01-26 ヒドロキシアパタイトクロマトグラフィーを用いる高分子量凝集体の取り出し

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US (2) US9469672B2 (https=)
EP (2) EP2336172B1 (https=)
JP (2) JP5004586B2 (https=)
KR (2) KR101221862B1 (https=)
CN (2) CN1898266B (https=)
AU (1) AU2004286938B2 (https=)
BR (2) BR122020007658B1 (https=)
CA (1) CA2543193C (https=)
CO (1) CO5690649A2 (https=)
DK (2) DK2336172T3 (https=)
EC (1) ECSP066583A (https=)
ES (2) ES2530446T3 (https=)
IL (1) IL175229A (https=)
MX (1) MXPA06004472A (https=)
NZ (1) NZ547315A (https=)
PL (2) PL1678208T3 (https=)
PT (2) PT1678208E (https=)
RU (2) RU2409591C2 (https=)
WO (1) WO2005044856A2 (https=)

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US20050107594A1 (en) 2005-05-19
JP5490156B2 (ja) 2014-05-14
CO5690649A2 (es) 2006-10-31
EP2336172B1 (en) 2014-12-10
RU2573910C2 (ru) 2016-01-27
AU2004286938A1 (en) 2005-05-19
RU2010139827A (ru) 2012-03-27
CA2543193A1 (en) 2005-05-19
EP1678208B1 (en) 2013-05-15
EP1678208A2 (en) 2006-07-12
PT2336172E (pt) 2015-02-20
PL2336172T3 (pl) 2015-04-30
WO2005044856A3 (en) 2005-06-23
CN1898266B (zh) 2011-09-21
PL1678208T3 (pl) 2013-09-30
DK2336172T3 (en) 2015-01-26
MXPA06004472A (es) 2006-06-20
BRPI0415887B1 (pt) 2021-01-26
CN1898266A (zh) 2007-01-17
WO2005044856A2 (en) 2005-05-19
ES2530446T3 (es) 2015-03-02
ECSP066583A (es) 2006-10-17
IL175229A (en) 2013-08-29
CN102276717A (zh) 2011-12-14
CA2543193C (en) 2015-08-11
RU2006113695A (ru) 2007-12-10
US20130197198A1 (en) 2013-08-01
KR20060120163A (ko) 2006-11-24
KR20120003027A (ko) 2012-01-09
ES2418830T3 (es) 2013-08-16
KR101321876B1 (ko) 2013-10-28
EP2336172A1 (en) 2011-06-22
NZ547315A (https=) 2008-07-31
KR101221862B1 (ko) 2013-01-14
IL175229A0 (en) 2006-09-05
BRPI0415887A (pt) 2007-01-09
BR122020007658B1 (pt) 2021-07-27
US9469672B2 (en) 2016-10-18
PT1678208E (pt) 2013-07-10
JP2007532477A (ja) 2007-11-15
RU2409591C2 (ru) 2011-01-20
AU2004286938B2 (en) 2011-06-30
DK1678208T3 (da) 2013-07-08
CN102276717B (zh) 2015-09-09
US9822143B2 (en) 2017-11-21
JP2012111769A (ja) 2012-06-14

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