ES2530446T3 - Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita - Google Patents

Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita Download PDF

Info

Publication number
ES2530446T3
ES2530446T3 ES10174366T ES10174366T ES2530446T3 ES 2530446 T3 ES2530446 T3 ES 2530446T3 ES 10174366 T ES10174366 T ES 10174366T ES 10174366 T ES10174366 T ES 10174366T ES 2530446 T3 ES2530446 T3 ES 2530446T3
Authority
ES
Spain
Prior art keywords
molecular weight
hydroxyapatite chromatography
high molecular
removal
weight aggregates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES10174366T
Other languages
English (en)
Spanish (es)
Inventor
Shujun Sun
Christopher Gallo
Brian Kelley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth LLC
Original Assignee
Wyeth LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth LLC filed Critical Wyeth LLC
Application granted granted Critical
Publication of ES2530446T3 publication Critical patent/ES2530446T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
ES10174366T 2003-10-27 2004-10-06 Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita Expired - Lifetime ES2530446T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51401803P 2003-10-27 2003-10-27
US52333503P 2003-11-20 2003-11-20

Publications (1)

Publication Number Publication Date
ES2530446T3 true ES2530446T3 (es) 2015-03-02

Family

ID=34576743

Family Applications (2)

Application Number Title Priority Date Filing Date
ES10174366T Expired - Lifetime ES2530446T3 (es) 2003-10-27 2004-10-06 Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita
ES04794288T Expired - Lifetime ES2418830T3 (es) 2003-10-27 2004-10-06 Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES04794288T Expired - Lifetime ES2418830T3 (es) 2003-10-27 2004-10-06 Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita

Country Status (19)

Country Link
US (2) US9469672B2 (https=)
EP (2) EP2336172B1 (https=)
JP (2) JP5004586B2 (https=)
KR (2) KR101221862B1 (https=)
CN (2) CN1898266B (https=)
AU (1) AU2004286938B2 (https=)
BR (2) BR122020007658B1 (https=)
CA (1) CA2543193C (https=)
CO (1) CO5690649A2 (https=)
DK (2) DK2336172T3 (https=)
EC (1) ECSP066583A (https=)
ES (2) ES2530446T3 (https=)
IL (1) IL175229A (https=)
MX (1) MXPA06004472A (https=)
NZ (1) NZ547315A (https=)
PL (2) PL1678208T3 (https=)
PT (2) PT1678208E (https=)
RU (2) RU2409591C2 (https=)
WO (1) WO2005044856A2 (https=)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
EP2336172B1 (en) * 2003-10-27 2014-12-10 Wyeth LLC Removal of high molecular weight aggregates using hydroxyapatite chromatography
AU2006223180B2 (en) * 2005-03-11 2011-11-24 Wyeth Llc A method of weak partitioning chromatography
TWI372763B (en) * 2005-06-17 2012-09-21 Wyeth Llc Methods of purifying anti a beta antibodies
EP1893647A2 (en) * 2005-06-23 2008-03-05 MedImmune, Inc. Antibody formulations having optimized aggregation and fragmentation profiles
AR058140A1 (es) * 2005-10-24 2008-01-23 Wyeth Corp Metodo de produccion proteica utilizando compuestos anti-senescencia
BRPI0620316A2 (pt) * 2005-12-21 2011-11-08 Wyeth Corp formulações de proteìnas com viscosidades reduzida e seus usos
EP2012118A4 (en) 2006-04-25 2009-12-16 Tosoh Corp ISOLENT FOR IGG PURIFICATION AND METHOD FOR PURIFYING AN IGG MONOMER THEREFOR
US20100234577A1 (en) * 2006-06-14 2010-09-16 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
DK2061803T4 (da) 2006-08-28 2022-12-05 Ares Trading Sa Fremgangsmåde til oprensning af fc-holdige proteiner
EA017733B1 (ru) * 2006-08-28 2013-02-28 Арес Трейдинг С.А. СПОСОБ ОЧИСТКИ Fc-СЛИТЫХ БЕЛКОВ
WO2008031020A2 (en) * 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
TWI456062B (zh) 2006-09-13 2014-10-11 Abbvie Inc 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US7691980B2 (en) 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7999085B2 (en) * 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
CA2674213A1 (en) * 2007-01-26 2008-07-31 Merck Serono S.A. Purification of fc-taci fusion proteins using the oilbody technology
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
CA2703086C (en) * 2007-10-26 2014-11-25 Asahi Kasei Chemicals Corporation Method for purifying protein
US8188242B2 (en) 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
MX2011001506A (es) † 2008-08-14 2011-03-15 Genentech Inc Metodos para eliminar un contaminante utilizado cromatografia de membrana de intercambio de ion en desplazamiento de proteina indigena.
MX2011004201A (es) 2008-10-20 2011-05-24 Abbott Lab Desactivacion viral durante purificacion de anticuerpos.
AU2009347206C1 (en) 2008-10-20 2016-12-08 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
BRPI0919879A2 (pt) * 2008-10-29 2016-02-16 Wyeth Llc métodos para purificação de moléculas de ligação a antígeno de domínio único
EP2362767B1 (en) 2008-10-29 2017-12-06 Ablynx N.V. Formulations of single domain antigen binding molecules
EP2350130B1 (en) * 2008-10-31 2018-10-03 Wyeth LLC Purification of acidic proteins using ceramic hydroxyapatite chromatography
CN102395597A (zh) * 2009-03-11 2012-03-28 惠氏有限责任公司 纯化小模块免疫药物蛋白的方法
US8481694B2 (en) 2009-04-29 2013-07-09 Bio-Rad Laboratories, Inc. Purification of immunoconjugates
JPWO2011001963A1 (ja) 2009-07-03 2012-12-13 旭化成ケミカルズ株式会社 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法
ES2813398T3 (es) 2009-10-20 2021-03-23 Abbvie Inc Aislamiento y purificación de anticuerpos anti-IL-13 usando cromatografía de afinidad a Proteína A
EP2513134B1 (en) 2009-12-18 2017-09-06 Novartis AG Wash solution and method for affinity chromatography
WO2011090720A2 (en) * 2009-12-29 2011-07-28 Dr. Reddy's Laboratories Ltd Purification of proteins
WO2011088225A1 (en) 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
RU2012146448A (ru) 2010-04-14 2014-05-20 Ф.Хоффманн-Ля Рош Аг Удаление агрегатов иммуноглобулинов
GB201006753D0 (en) 2010-04-22 2010-06-09 Biotest Ag Process for preparing an immunolobulin composition
WO2011156073A1 (en) * 2010-06-08 2011-12-15 Millipore Corporation Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts
US8895707B2 (en) * 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
CA2814781A1 (en) 2010-11-01 2012-05-10 Diderik Reinder Kremer Single unit ion exchange chromatography antibody purification
JP5881744B2 (ja) * 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
SG195306A1 (en) * 2011-06-08 2013-12-30 Agency Science Tech & Res Purification of biological products by constrained cohydration chromatography
US20140187749A1 (en) 2011-06-16 2014-07-03 Dsm Ip Assets B.V. Single unit chromatography antibody purification
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
WO2014003142A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 抗体
JP2014105180A (ja) * 2012-11-27 2014-06-09 Hoya Corp モノマー化方法
SG10201707477SA (en) 2013-03-13 2017-10-30 Eleven Biotherapeutics Inc Chimeric cytokine formulations for ocular delivery
CA2909969A1 (en) * 2013-05-13 2014-12-31 Medimmune, Llc Separation of recombinant polyclonal antibody multimers with minimal separation of monomers
MX384222B (es) 2013-07-05 2025-03-14 Lab Francais Du Fractionnement Matriz de cromatografia de afinidad.
WO2015005960A1 (en) 2013-07-12 2015-01-15 Emd Millipore Corporation Removal of fragments from a sample containing a target protein using activated carbon
JP6479305B2 (ja) * 2013-07-12 2019-03-06 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
BR112016014824A2 (pt) * 2013-12-27 2017-09-19 Chugai Pharmaceutical Co Ltd Método para purificar anticorpo que tem ponto isoelétrico baixo
CN105392569B (zh) * 2014-06-23 2019-08-20 生物辐射实验室股份有限公司 磷灰石预处理
US10099157B2 (en) 2014-06-23 2018-10-16 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
EP3233230B1 (en) 2014-12-15 2024-06-12 Merck Patent GmbH Target molecule capture from crude solutions
KR102490956B1 (ko) 2015-03-13 2023-01-19 브리스톨-마이어스 스큅 컴퍼니 불순물을 제거하기 위한 크로마토그래피 동안의 알칼리성 세척의 용도
US11661438B2 (en) 2015-12-21 2023-05-30 Pfizer, Inc. Purification of antibody drug conjugates using a sodium phosphate gradient
US11919924B1 (en) * 2016-03-15 2024-03-05 Fresenius Kabi Deutschland Gmbh Methods of purifying and producing an adalimumab antibody
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
CN106977591B (zh) * 2017-05-04 2020-05-05 广州康盛生物科技股份有限公司 一种分离纯化重组葡萄球菌蛋白a的方法
WO2019040671A1 (en) * 2017-08-22 2019-02-28 Biogen Ma Inc. METHODS OF PURIFYING ANTIBODIES HAVING REDUCED AGGREGATES OF HIGH MOLECULAR WEIGHT
WO2019116096A1 (en) * 2017-12-15 2019-06-20 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Production of fc fragments
MX2020008931A (es) * 2018-02-27 2020-10-01 Pfizer Purificacion de anticuerpos.
CN111902720B (zh) * 2018-03-21 2025-02-07 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
JP2021519339A (ja) 2018-04-03 2021-08-10 メルク パテント ゲーエムベーハー Ｃｅｘクロマトグラフィー媒体及び生物製剤供給原料からの標的タンパク質の低塩溶出
WO2019224660A1 (ja) * 2018-05-24 2019-11-28 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法
JP6704074B2 (ja) * 2019-02-04 2020-06-03 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
IL293047A (en) 2019-11-22 2022-07-01 Morphosys Ag A method for increasing antibody yield during ion exchange chromatography
JP2020099909A (ja) * 2020-03-17 2020-07-02 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 処理方法、生産方法およびハイドロキシアパタイト充填剤
IL296767A (en) 2020-03-30 2022-11-01 Ablynx Nv A method for the production and purification of single variable multivalent immunoglobulin sites
EP4142792A4 (en) * 2020-05-01 2024-11-27 Kashiv Biosciences, LLC An improved process of purification of protein

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US428894A (en) 1890-05-27 Process of making tool-handles
US523335A (en) 1894-07-24 Air-bolt for flour-mills
US514018A (en) 1894-02-06 Edward j
US419964A (en) 1890-01-21 Transom-lifter
US4745183A (en) * 1984-02-21 1988-05-17 Bio-Rad Laboratories, Inc. Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
JPH03291300A (ja) * 1989-12-28 1991-12-20 Mitsui Toatsu Chem Inc ポリクローナル抗体の分離方法
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
TWI255272B (en) * 2000-12-06 2006-05-21 Guriq Basi Humanized antibodies that recognize beta amyloid peptide
WO2003059935A2 (en) 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
AU2004215653B2 (en) 2003-02-28 2011-03-17 Lonza Biologics Plc. Antibody purification by protein A and ion exchange chromatography
EP2336172B1 (en) * 2003-10-27 2014-12-10 Wyeth LLC Removal of high molecular weight aggregates using hydroxyapatite chromatography

Also Published As

Publication number Publication date
US20050107594A1 (en) 2005-05-19
JP5490156B2 (ja) 2014-05-14
CO5690649A2 (es) 2006-10-31
EP2336172B1 (en) 2014-12-10
RU2573910C2 (ru) 2016-01-27
AU2004286938A1 (en) 2005-05-19
RU2010139827A (ru) 2012-03-27
CA2543193A1 (en) 2005-05-19
EP1678208B1 (en) 2013-05-15
EP1678208A2 (en) 2006-07-12
PT2336172E (pt) 2015-02-20
PL2336172T3 (pl) 2015-04-30
WO2005044856A3 (en) 2005-06-23
CN1898266B (zh) 2011-09-21
PL1678208T3 (pl) 2013-09-30
DK2336172T3 (en) 2015-01-26
MXPA06004472A (es) 2006-06-20
BRPI0415887B1 (pt) 2021-01-26
CN1898266A (zh) 2007-01-17
JP5004586B2 (ja) 2012-08-22
WO2005044856A2 (en) 2005-05-19
ECSP066583A (es) 2006-10-17
IL175229A (en) 2013-08-29
CN102276717A (zh) 2011-12-14
CA2543193C (en) 2015-08-11
RU2006113695A (ru) 2007-12-10
US20130197198A1 (en) 2013-08-01
KR20060120163A (ko) 2006-11-24
KR20120003027A (ko) 2012-01-09
ES2418830T3 (es) 2013-08-16
KR101321876B1 (ko) 2013-10-28
EP2336172A1 (en) 2011-06-22
NZ547315A (https=) 2008-07-31
KR101221862B1 (ko) 2013-01-14
IL175229A0 (en) 2006-09-05
BRPI0415887A (pt) 2007-01-09
BR122020007658B1 (pt) 2021-07-27
US9469672B2 (en) 2016-10-18
PT1678208E (pt) 2013-07-10
JP2007532477A (ja) 2007-11-15
RU2409591C2 (ru) 2011-01-20
AU2004286938B2 (en) 2011-06-30
DK1678208T3 (da) 2013-07-08
CN102276717B (zh) 2015-09-09
US9822143B2 (en) 2017-11-21
JP2012111769A (ja) 2012-06-14

Similar Documents

Publication Publication Date Title
ES2530446T3 (es) Retirada de agregados de alto peso molecular usando cromatografía de hidroxiapatita
BR9807521A (https=)
BR0211470A (pt) Sistema de reciclagem de gás, e, processo para a purificação e a reciclagem de um gás
DK0994728T3 (da) Rekombinante antistoffer, som er specifikke for beta-amyloide ender, DNA, der koder derfor, samt fremgangsmåder til anvendelse heraf
ATE425989T1 (de) Proteinreinigungsverfahren
WO2003104418A3 (en) Reconstituted polypeptides
JP2002539272A5 (https=)
DE69939312D1 (de) Verfahren zur identifizierung von induktoren und inhibitoren proteolytischer antikörper, zusammensetzungen sowie deren verwendungen
EA200200503A1 (ru) Способ лечения ревматоидного артрита
MXPA01012662A (es) Deteccion temprana de flavivirus que utiliza la glicoproteina ns1.
ES2078947T3 (es) Purificacion del complejo del factor viii utilizando cromatografia de afinidad con heparina.
BRPI0510567A (pt) anticorpos
PT1113817E (pt) Processo e materiais para a rapida deteccao de streptococcus pneumoniae utilizando anticorpos purificados especificos para o antigenio
ATE312909T1 (de) Diagnostika und behandlungen von peridontalen erkrankungen
BR0006676B1 (pt) dispositivo que permite separar pelo menos um composto a partir de uma mistura ou de um corpo por adsorção e processo de injeção de fluido desviado em um processo de separação por leito móvel simulado.
ATE162311T1 (de) Immunoassay zum nachweis von kollagen oder kollagenfragmenten
PT656014E (pt) Proteina rib, uma proteina da superficie celular que confere imunidade a muitas estirpes de estreptococos do grupo b: processo para purificacao da proteina, estojo de reagentes e composicao farmaceutica
EA200600459A1 (ru) Способ получения цитотоксических лимфоцитов
BR0215588A (pt) Compostos imunoefetores
EP1308730A4 (en) PROCEDURE FOR DETECTING OR DETERMINING HBV
BR0314654A (pt) Método de determinação de grupamentos especìficos que constituem as heparinas ou as heparinas de baixo peso molecular
Kobayashi et al. Studies on human secretory IgA (II). Comparative studies on a fragment of secretory component derived from secretory IgA and fragments obtained by enzymatic digestion of free secretory component
PT816377E (pt) Peptideos que se ligam a protrombina e a trombina
KR890002228A (ko) 태반조직 단백질 pp 4의 정제방법
ES2084599T3 (es) Metodo para purificar antitrombina iii y preparacion de antitrombina iii que contiene dicha antitrombina iii.