JP2010504738A5 - - Google Patents
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- Publication number
- JP2010504738A5 JP2010504738A5 JP2009529350A JP2009529350A JP2010504738A5 JP 2010504738 A5 JP2010504738 A5 JP 2010504738A5 JP 2009529350 A JP2009529350 A JP 2009529350A JP 2009529350 A JP2009529350 A JP 2009529350A JP 2010504738 A5 JP2010504738 A5 JP 2010504738A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- anion exchanger
- resin
- eluent
- potassium carbonate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82691306P | 2006-09-26 | 2006-09-26 | |
| US60/826,913 | 2006-09-26 | ||
| PCT/US2007/078816 WO2008039664A1 (en) | 2006-09-26 | 2007-09-19 | Nucleic acid purification method using anion exchange |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010504738A JP2010504738A (ja) | 2010-02-18 |
| JP2010504738A5 true JP2010504738A5 (https=) | 2011-10-20 |
| JP5265552B2 JP5265552B2 (ja) | 2013-08-14 |
Family
ID=38640520
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009529350A Active JP5265552B2 (ja) | 2006-09-26 | 2007-09-19 | 陰イオン交換を用いる核酸精製方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7655792B2 (https=) |
| EP (1) | EP2066792B1 (https=) |
| JP (1) | JP5265552B2 (https=) |
| GB (1) | GB2445442A (https=) |
| WO (1) | WO2008039664A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2250182B1 (en) * | 2008-02-07 | 2015-12-02 | GE Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| EP2315839A4 (en) * | 2008-08-25 | 2012-03-21 | Ge Healthcare Bio Sciences | EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA |
| WO2012002887A1 (en) | 2010-06-29 | 2012-01-05 | Sjoeblom Tobias | Method and kit for sequential isolation of nucleotide species from a sample |
| JP6061381B2 (ja) * | 2011-01-12 | 2017-01-18 | 積水メディカル株式会社 | 一塩基多型の検出方法 |
| US9481881B2 (en) | 2011-01-12 | 2016-11-01 | Sekisui Medical Co., Ltd. | Eluent for ion-exchange chromatography, and method of analyzing nucleic acid chains |
| AU2012236099A1 (en) | 2011-03-31 | 2013-10-03 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
| WO2014152027A1 (en) | 2013-03-15 | 2014-09-25 | Moderna Therapeutics, Inc. | Manufacturing methods for production of rna transcripts |
| US10590161B2 (en) | 2013-03-15 | 2020-03-17 | Modernatx, Inc. | Ion exchange purification of mRNA |
| WO2014152031A1 (en) | 2013-03-15 | 2014-09-25 | Moderna Therapeutics, Inc. | Ribonucleic acid purification |
| EP2971165A4 (en) | 2013-03-15 | 2016-11-23 | Moderna Therapeutics Inc | DISSOLUTION OF DNA FRAGMENTS IN MRNA MANUFACTURING METHODS |
| EP3052511A4 (en) | 2013-10-02 | 2017-05-31 | Moderna Therapeutics, Inc. | Polynucleotide molecules and uses thereof |
| WO2015196128A2 (en) | 2014-06-19 | 2015-12-23 | Moderna Therapeutics, Inc. | Alternative nucleic acid molecules and uses thereof |
| US10407683B2 (en) | 2014-07-16 | 2019-09-10 | Modernatx, Inc. | Circular polynucleotides |
| WO2017049286A1 (en) | 2015-09-17 | 2017-03-23 | Moderna Therapeutics, Inc. | Polynucleotides containing a morpholino linker |
| EP3350333B2 (en) | 2015-09-17 | 2025-08-06 | ModernaTX, Inc. | Polynucleotides containing a stabilizing tail region |
| WO2017223176A1 (en) | 2016-06-24 | 2017-12-28 | Modernatx, Inc. | Methods and apparatus for filtration |
| EP4070102A1 (en) | 2019-12-05 | 2022-10-12 | Waters Technologies Corporation | Polyphosphonic acids for improving recovery and minimizing system loss |
| SI3896159T1 (sl) * | 2020-04-17 | 2025-08-29 | Sartorius Bia Separations D.O.O. | Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca |
| EP4182460A1 (en) * | 2020-07-14 | 2023-05-24 | Waters Technologies Corporation | Kits and methods useful for separating oligonucleotides from matrix components |
| CN121941767A (zh) * | 2023-08-02 | 2026-04-28 | 默克专利股份公司 | 用于纯化质粒dna的方法 |
| WO2025257280A1 (en) * | 2024-06-14 | 2025-12-18 | Merck Patent Gmbh | Method for purification of rna |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4034036C2 (de) * | 1990-10-26 | 1994-03-03 | Diagen Inst Molekularbio | Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen |
| US5866429A (en) * | 1991-04-03 | 1999-02-02 | Bloch; Will | Precision and accuracy of anion-exchange separation of nucleic acids |
| DE4139664A1 (de) | 1991-12-02 | 1993-06-03 | Diagen Inst Molekularbio | Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5990301A (en) * | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| ATE223059T1 (de) * | 1994-03-11 | 2002-09-15 | Abbott Lab | Cyanidfreies reagens und verfahren zur bestimmung von hämoglobin |
| SE9500183D0 (sv) * | 1995-01-20 | 1995-01-20 | Pharmacia Biotech Ab | Method for the purifivation of short nucleic acids |
| SE9600590D0 (sv) * | 1996-02-19 | 1996-02-19 | Pharmacia Biotech Ab | Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris |
| US5707812A (en) * | 1996-08-06 | 1998-01-13 | Vical Incorporated | Purification of plasmid DNA during column chromatography |
| SE9703532D0 (sv) * | 1997-09-30 | 1997-09-30 | Pharmacia Biotech Ab | A process for the purification of plasmid DNA |
| EP0992583B1 (en) | 1998-09-14 | 2003-03-26 | QIAGEN GmbH | Novel method for purifying covalently closed circular DNA |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| ATE278699T1 (de) | 2000-02-16 | 2004-10-15 | Macherey Nagel Gmbh & Co Hg | Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens |
| EP1243649A1 (de) * | 2001-03-23 | 2002-09-25 | GeneScan Europe AG | Verfahren zur Isolierung von DNA |
| AU2003216830A1 (en) * | 2002-03-21 | 2003-10-08 | Avecia Biotechnology Inc. | Purification methods for oligonucleotides and their analogs |
| US7964343B2 (en) * | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
-
2007
- 2007-09-10 GB GB0717591A patent/GB2445442A/en not_active Withdrawn
- 2007-09-19 JP JP2009529350A patent/JP5265552B2/ja active Active
- 2007-09-19 US US11/857,468 patent/US7655792B2/en active Active
- 2007-09-19 WO PCT/US2007/078816 patent/WO2008039664A1/en not_active Ceased
- 2007-09-19 EP EP07853559.8A patent/EP2066792B1/en active Active
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