JP5265552B2 - 陰イオン交換を用いる核酸精製方法 - Google Patents

陰イオン交換を用いる核酸精製方法 Download PDF

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Publication number
JP5265552B2
JP5265552B2 JP2009529350A JP2009529350A JP5265552B2 JP 5265552 B2 JP5265552 B2 JP 5265552B2 JP 2009529350 A JP2009529350 A JP 2009529350A JP 2009529350 A JP2009529350 A JP 2009529350A JP 5265552 B2 JP5265552 B2 JP 5265552B2
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Prior art keywords
nucleic acid
genomic dna
resin
anion exchanger
eluent
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Japanese (ja)
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JP2010504738A5 (https=
JP2010504738A (ja
Inventor
タッケラッパティ，スッダカール・ラオ
アンバット，ラジェッシュ
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Global Life Sciences Solutions USA LLC
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Amersham Biosciences Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2009529350A 2006-09-26 2007-09-19 陰イオン交換を用いる核酸精製方法 Active JP5265552B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82691306P 2006-09-26 2006-09-26
US60/826,913 2006-09-26
PCT/US2007/078816 WO2008039664A1 (en) 2006-09-26 2007-09-19 Nucleic acid purification method using anion exchange

Publications (3)

Publication Number Publication Date
JP2010504738A JP2010504738A (ja) 2010-02-18
JP2010504738A5 JP2010504738A5 (https=) 2011-10-20
JP5265552B2 true JP5265552B2 (ja) 2013-08-14

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ID=38640520

Family Applications (1)

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JP2009529350A Active JP5265552B2 (ja) 2006-09-26 2007-09-19 陰イオン交換を用いる核酸精製方法

Country Status (5)

Country Link
US (1) US7655792B2 (https=)
EP (1) EP2066792B1 (https=)
JP (1) JP5265552B2 (https=)
GB (1) GB2445442A (https=)
WO (1) WO2008039664A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8067580B2 (en) * 2008-02-07 2011-11-29 Ge Healthcare Bio-Sciences Corp. Isolation of DNA, RNA and protein from a single sample
EP2315839A4 (en) * 2008-08-25 2012-03-21 Ge Healthcare Bio Sciences EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA
EP2588609B1 (en) 2010-06-29 2018-01-17 Exscale Biospecimen Solutions AB Method and kit for sequential isolation of nucleotide species from a sample
CN103314290B (zh) 2011-01-12 2015-07-01 积水医疗株式会社 核酸链的分离方法
WO2012096329A1 (ja) * 2011-01-12 2012-07-19 積水メディカル株式会社 一塩基多型の検出方法
EP2691101A2 (en) 2011-03-31 2014-02-05 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
WO2014152031A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Ribonucleic acid purification
WO2014152027A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Manufacturing methods for production of rna transcripts
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
WO2015051169A2 (en) 2013-10-02 2015-04-09 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
WO2015196128A2 (en) 2014-06-19 2015-12-23 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
US10407683B2 (en) 2014-07-16 2019-09-10 Modernatx, Inc. Circular polynucleotides
PL3350333T3 (pl) 2015-09-17 2022-03-07 Modernatx, Inc. Polinukleotydy zawierające region stabilizujący ogon
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
WO2017223176A1 (en) 2016-06-24 2017-12-28 Modernatx, Inc. Methods and apparatus for filtration
EP4070102A1 (en) 2019-12-05 2022-10-12 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
EP3896159B1 (en) * 2020-04-17 2025-05-28 Sartorius BIA Separations d.o.o. A method of single strand rna purification employing an anion exchanger
EP4182460A1 (en) * 2020-07-14 2023-05-24 Waters Technologies Corporation Kits and methods useful for separating oligonucleotides from matrix components
KR20260046476A (ko) * 2023-08-02 2026-04-07 메르크 파텐트 게엠베하 플라스미드 dna의 정제 방법
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna

Family Cites Families (16)

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Publication number Priority date Publication date Assignee Title
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5866429A (en) 1991-04-03 1999-02-02 Bloch; Will Precision and accuracy of anion-exchange separation of nucleic acids
DE4139664A1 (de) 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
US5561064A (en) 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5990301A (en) 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
EP0749583B1 (en) 1994-03-11 2002-08-28 Abbott Laboratories Cyanide-free reagent and method for the determination of hemoglobin
SE9500183D0 (sv) 1995-01-20 1995-01-20 Pharmacia Biotech Ab Method for the purifivation of short nucleic acids
SE9600590D0 (sv) * 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
EP0992583B1 (en) 1998-09-14 2003-03-26 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
DE50008101D1 (de) 2000-02-16 2004-11-11 Macherey Nagel Gmbh & Co Hg Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
EP1243649A1 (de) * 2001-03-23 2002-09-25 GeneScan Europe AG Verfahren zur Isolierung von DNA
CN1643145A (zh) * 2002-03-21 2005-07-20 艾夫西亚生物科技公司 寡核苷酸及其类似物的纯化方法
US7964343B2 (en) * 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture

Also Published As

Publication number Publication date
EP2066792B1 (en) 2014-03-12
WO2008039664A1 (en) 2008-04-03
US7655792B2 (en) 2010-02-02
GB0717591D0 (en) 2007-10-17
US20080076910A1 (en) 2008-03-27
GB2445442A (en) 2008-07-09
EP2066792A1 (en) 2009-06-10
JP2010504738A (ja) 2010-02-18

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