JP2015515969A5 - - Google Patents
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- Publication number
- JP2015515969A5 JP2015515969A5 JP2015509441A JP2015509441A JP2015515969A5 JP 2015515969 A5 JP2015515969 A5 JP 2015515969A5 JP 2015509441 A JP2015509441 A JP 2015509441A JP 2015509441 A JP2015509441 A JP 2015509441A JP 2015515969 A5 JP2015515969 A5 JP 2015515969A5
- Authority
- JP
- Japan
- Prior art keywords
- organic target
- protein
- fusion protein
- recombinant fusion
- affinity medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 11
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 11
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 230000009834 selective interaction Effects 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 239000012634 fragment Substances 0.000 claims 3
- 102000037865 fusion proteins Human genes 0.000 claims 3
- 108020001507 fusion proteins Proteins 0.000 claims 3
- 102000009027 Albumins Human genes 0.000 claims 2
- 108010088751 Albumins Proteins 0.000 claims 2
- 108060003951 Immunoglobulin Proteins 0.000 claims 2
- 229920001872 Spider silk Polymers 0.000 claims 2
- 108010088160 Staphylococcal Protein A Proteins 0.000 claims 2
- 108700011201 Streptococcus IgG Fc-binding Proteins 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 102000018358 immunoglobulin Human genes 0.000 claims 2
- 229940072221 immunoglobulins Drugs 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 102000002110 C2 domains Human genes 0.000 claims 1
- 108050009459 C2 domains Proteins 0.000 claims 1
- 108010022355 Fibroins Proteins 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 210000004900 c-terminal fragment Anatomy 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 230000003252 repetitive effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12166393 | 2012-05-02 | ||
| EP12166393.4 | 2012-05-02 | ||
| PCT/EP2013/059146 WO2013164405A1 (en) | 2012-05-02 | 2013-05-02 | Spider silk fusion protein structures without repetitive fragment for binding to an organic target |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015515969A JP2015515969A (ja) | 2015-06-04 |
| JP2015515969A5 true JP2015515969A5 (https=) | 2016-06-23 |
| JP6412866B2 JP6412866B2 (ja) | 2018-10-24 |
Family
ID=48289167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015509441A Active JP6412866B2 (ja) | 2012-05-02 | 2013-05-02 | 有機ターゲットに結合するための反復断片を持たないクモ糸融合タンパク質の構造 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9708376B2 (https=) |
| EP (1) | EP2844360B1 (https=) |
| JP (1) | JP6412866B2 (https=) |
| CN (1) | CN104394953B (https=) |
| AU (1) | AU2013255815B2 (https=) |
| CA (1) | CA2872056A1 (https=) |
| WO (1) | WO2013164405A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101657298B1 (ko) * | 2016-02-03 | 2016-09-13 | (주)넥스젠바이오텍 | 세포 증식 효과가 증가한 인간 상피세포재생인자 융합단백질 및 이를 유효성분으로 함유하는 피부 주름 및 탄력 개선용 화장료 조성물 |
| KR102790669B1 (ko) | 2016-02-11 | 2025-04-04 | 시비스 머테리얼 사이언스 리미티드 | 합성 드래그라인 거미 실크를 포함하는 복합체 |
| EP3263593A1 (en) * | 2016-07-01 | 2018-01-03 | Anna Rising | Engineered spider silk proteins and uses thereof |
| CN111094326B (zh) * | 2017-06-13 | 2025-03-04 | 阿尔托大学基金会 | 用于产生丝融合蛋白的浓缩粘合相的方法 |
| US20230201413A1 (en) * | 2019-03-11 | 2023-06-29 | Seevix Material Sciences Ltd. | Compositions comprising dragline spider silk |
| CA3135846A1 (en) * | 2019-04-02 | 2020-10-08 | Bondwell Technologies Inc. | Functionalized ubx protein materials for enhanced purification of antibodies |
| EP4139338A4 (en) | 2020-04-23 | 2025-07-09 | Seevix Mat Sciences Ltd | MODIFIED SPIDER SILK FIBERS AND THEIR USE |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69433299T2 (de) * | 1993-06-15 | 2004-09-09 | E.I. Du Pont De Nemours And Co., Wilmington | Rekombinante spinnerseide analoge |
| CA2712304C (en) | 1994-06-10 | 2011-10-25 | United States Surgical Corporation | Recombinant chimeric proteins and methods of use thereof |
| JP2000125872A (ja) | 1998-09-07 | 2000-05-09 | Terumo Corp | 三量体キメラタンパク質およびキメラタンパク質を含有するコラーゲンマトリックス |
| DE19932688B4 (de) | 1999-07-13 | 2009-10-08 | Scil Proteins Gmbh | Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen |
| GB9917027D0 (en) | 1999-07-20 | 1999-09-22 | Affibody Technology Sweeden Ab | In vitro selection and optional identification of polypeptides using solid support carriers |
| US20050261479A1 (en) | 2004-04-29 | 2005-11-24 | Christian Hoffmann | Method for purifying and recovering silk proteins using magnetic affinity separation |
| US7960509B2 (en) | 2005-01-14 | 2011-06-14 | Trustees Of Tufts College | Fibrous protein fusions and use thereof in the formation of advanced organic/inorganic composite materials |
| EP2899204B1 (en) * | 2005-12-30 | 2017-06-28 | Spiber Technologies AB | Support for cell culture comprising spider silk proteins and methods for producing the same |
| EP1852470A1 (en) | 2006-05-03 | 2007-11-07 | Technische Universität München | Multilayer Silk Protein Films |
| JP2012518398A (ja) | 2009-02-24 | 2012-08-16 | グラクソ グループ リミテッド | 抗原結合性構築物 |
| CA2759465C (en) * | 2009-04-22 | 2017-12-12 | Spiber Technologies Ab | Method of producing polymers of spider silk proteins |
| AU2010348409B2 (en) * | 2010-03-18 | 2014-03-20 | Spiber Technologies Ab | Production of proteins and polypeptides |
| US9157070B2 (en) * | 2010-04-12 | 2015-10-13 | Spiber Technologies Ab | Methods and combination comprising eukaryotic cells and recombinant spider silk protein |
-
2013
- 2013-05-02 CA CA2872056A patent/CA2872056A1/en not_active Abandoned
- 2013-05-02 AU AU2013255815A patent/AU2013255815B2/en not_active Ceased
- 2013-05-02 EP EP13720382.4A patent/EP2844360B1/en active Active
- 2013-05-02 CN CN201380034941.0A patent/CN104394953B/zh active Active
- 2013-05-02 US US14/398,370 patent/US9708376B2/en active Active
- 2013-05-02 JP JP2015509441A patent/JP6412866B2/ja active Active
- 2013-05-02 WO PCT/EP2013/059146 patent/WO2013164405A1/en not_active Ceased
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