JP2016513643A5 - - Google Patents
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- Publication number
- JP2016513643A5 JP2016513643A5 JP2016501314A JP2016501314A JP2016513643A5 JP 2016513643 A5 JP2016513643 A5 JP 2016513643A5 JP 2016501314 A JP2016501314 A JP 2016501314A JP 2016501314 A JP2016501314 A JP 2016501314A JP 2016513643 A5 JP2016513643 A5 JP 2016513643A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- recombinant protein
- tentacle
- chromatography medium
- anion exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 21
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 14
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 14
- 239000012501 chromatography medium Substances 0.000 claims 12
- 238000005349 anion exchange Methods 0.000 claims 10
- 239000011159 matrix material Substances 0.000 claims 10
- 102000004169 proteins and genes Human genes 0.000 claims 8
- 108090000623 proteins and genes Proteins 0.000 claims 8
- 239000003480 eluent Substances 0.000 claims 3
- 239000012149 elution buffer Substances 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 2
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000002952 polymeric resin Substances 0.000 claims 2
- 229920003002 synthetic resin Polymers 0.000 claims 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 2
- 108010008165 Etanercept Proteins 0.000 claims 1
- 239000012554 Fractogel® EMD TMAE Hicap (M) Substances 0.000 claims 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 claims 1
- 101710120037 Toxin CcdB Proteins 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 claims 1
- 238000001261 affinity purification Methods 0.000 claims 1
- 125000000129 anionic group Chemical group 0.000 claims 1
- 150000001450 anions Chemical group 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 229960000403 etanercept Drugs 0.000 claims 1
- 125000000524 functional group Chemical group 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 102000003298 tumor necrosis factor receptor Human genes 0.000 claims 1
- 229920002554 vinyl polymer Polymers 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361785038P | 2013-03-14 | 2013-03-14 | |
| US61/785,038 | 2013-03-14 | ||
| PCT/US2014/023682 WO2014159441A1 (en) | 2013-03-14 | 2014-03-11 | Removal of leaked affinity purification ligand |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016513643A JP2016513643A (ja) | 2016-05-16 |
| JP2016513643A5 true JP2016513643A5 (https=) | 2017-03-30 |
| JP6457479B2 JP6457479B2 (ja) | 2019-01-23 |
Family
ID=50424772
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016501314A Active JP6457479B2 (ja) | 2013-03-14 | 2014-03-11 | 漏出したアフィニティ精製リガンドの除去 |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US20160024144A1 (https=) |
| EP (3) | EP3395423B1 (https=) |
| JP (1) | JP6457479B2 (https=) |
| AU (1) | AU2014240596B2 (https=) |
| CA (1) | CA2904411A1 (https=) |
| CY (1) | CY1120353T1 (https=) |
| DK (2) | DK3395423T3 (https=) |
| ES (2) | ES2964601T3 (https=) |
| FI (1) | FI3395423T3 (https=) |
| HU (2) | HUE064450T2 (https=) |
| LT (2) | LT3395423T (https=) |
| MX (2) | MX391507B (https=) |
| PL (2) | PL3395423T3 (https=) |
| PT (2) | PT3395423T (https=) |
| SI (2) | SI3395423T1 (https=) |
| TR (1) | TR201809050T4 (https=) |
| WO (1) | WO2014159441A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3395423B1 (en) | 2013-03-14 | 2023-09-20 | Amgen Inc. | Removal of leaked affinity purification ligand |
| AR096713A1 (es) * | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
| AU2015273049B2 (en) * | 2014-06-13 | 2019-11-14 | Lupin Limited | Process for the purification of TNFR:Fc fusion protein |
| CN105214341B (zh) * | 2015-09-17 | 2017-12-15 | 广州康盛生物科技有限公司 | 组合型吸附柱及其制备方法 |
| WO2017168296A1 (en) * | 2016-03-29 | 2017-10-05 | Navya Biologicals Pvt. Ltd | A process for purification of fc-fusion proteins |
| US12516102B1 (en) | 2017-02-09 | 2026-01-06 | Bracco Suisse Sa | Process for the purification of soluble PSGL-1 protein variants |
| CN118047854B (zh) * | 2024-02-26 | 2024-08-02 | 武汉鹰达生物科技有限公司 | 一种人载脂蛋白a-i的分离纯化方法 |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6936694B1 (en) | 1982-05-06 | 2005-08-30 | Intermune, Inc. | Manufacture and expression of large structural genes |
| AU588819B2 (en) | 1984-10-29 | 1989-09-28 | Immunex Corporation | Cloning of human granulocyte-macrophage colony stimulating factor gene |
| US4968607A (en) | 1987-11-25 | 1990-11-06 | Immunex Corporation | Interleukin-1 receptors |
| DE3811042A1 (de) | 1988-03-31 | 1989-10-19 | Merck Patent Gmbh | Ionenaustauscher |
| US5075222A (en) | 1988-05-27 | 1991-12-24 | Synergen, Inc. | Interleukin-1 inhibitors |
| WO1990005183A1 (en) | 1988-10-31 | 1990-05-17 | Immunex Corporation | Interleukin-4 receptors |
| US5395760A (en) | 1989-09-05 | 1995-03-07 | Immunex Corporation | DNA encoding tumor necrosis factor-α and -β receptors |
| ATE194384T1 (de) | 1989-09-12 | 2000-07-15 | Hoffmann La Roche | Tnf-bindende proteine |
| US6204363B1 (en) | 1989-10-16 | 2001-03-20 | Amgen Inc. | Stem cell factor |
| US5272064A (en) | 1989-12-19 | 1993-12-21 | Amgen Inc. | DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof |
| US5149792A (en) | 1989-12-19 | 1992-09-22 | Amgen Inc. | Platelet-derived growth factor B chain analogs |
| AU651596B2 (en) | 1990-06-05 | 1994-07-28 | Immunex Corporation | Type II interleukin-1 receptors |
| US5350683A (en) | 1990-06-05 | 1994-09-27 | Immunex Corporation | DNA encoding type II interleukin-1 receptors |
| ATE240394T1 (de) | 1992-10-23 | 2003-05-15 | Immunex Corp | Methoden zur herstellung löslicher, oligomerer proteine |
| US5554512A (en) | 1993-05-24 | 1996-09-10 | Immunex Corporation | Ligands for flt3 receptors |
| DE4333821A1 (de) | 1993-10-04 | 1995-04-06 | Merck Patent Gmbh | Ionenaustauscher |
| US5981713A (en) | 1994-10-13 | 1999-11-09 | Applied Research Systems Ars Holding N.V. | Antibodies to intereleukin-1 antagonists |
| DE19501726A1 (de) | 1995-01-20 | 1996-07-25 | Merck Patent Gmbh | Polymerisationsfähige Derivate von Polyamiden |
| AU708239B2 (en) | 1995-06-29 | 1999-07-29 | Immunex Corporation | Cytokine that induces apoptosis |
| US6613544B1 (en) | 1995-12-22 | 2003-09-02 | Amgen Inc. | Osteoprotegerin |
| US6096728A (en) | 1996-02-09 | 2000-08-01 | Amgen Inc. | Composition and method for treating inflammatory diseases |
| WO1997049754A1 (de) | 1996-06-21 | 1997-12-31 | Merck Patent Gmbh | Polymerisationsfähige derivate von polyamiden |
| US6271349B1 (en) | 1996-12-23 | 2001-08-07 | Immunex Corporation | Receptor activator of NF-κB |
| US6235883B1 (en) | 1997-05-05 | 2001-05-22 | Abgenix, Inc. | Human monoclonal antibodies to epidermal growth factor receptor |
| EP0991940B1 (de) | 1997-06-18 | 2010-08-25 | Merck Patent GmbH | Verwendung monolithischer sorbentien für präparative chromatographische trennverfahren |
| US6337072B1 (en) | 1998-04-03 | 2002-01-08 | Hyseq, Inc. | Interleukin-1 receptor antagonist and recombinant production thereof |
| AU1920301A (en) | 1999-11-17 | 2001-05-30 | Immunex Corporation | Receptor activator of nf-kappa b |
| FR2824568B1 (fr) * | 2001-05-11 | 2004-04-09 | Lab Francais Du Fractionnement | Procede de preparation de concentres d'immunoglobulines humaines a usage therapeutique |
| WO2003059935A2 (en) * | 2001-12-21 | 2003-07-24 | Immunex Corporation | Methods for purifying protein |
| AU2006223180B2 (en) * | 2005-03-11 | 2011-11-24 | Wyeth Llc | A method of weak partitioning chromatography |
| KR20140071452A (ko) * | 2006-04-05 | 2014-06-11 | 애브비 바이오테크놀로지 리미티드 | 항체 정제 |
| ES2538986T3 (es) * | 2006-11-08 | 2015-06-25 | Wyeth Llc | Medios diseñados racionalmente para un cultivo celular |
| AU2009347206C1 (en) * | 2008-10-20 | 2016-12-08 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
| CN102395597A (zh) * | 2009-03-11 | 2012-03-28 | 惠氏有限责任公司 | 纯化小模块免疫药物蛋白的方法 |
| CA2808116C (en) * | 2010-07-30 | 2016-06-07 | Pfizer Inc. | Tandem purification of proteins |
| WO2012176158A1 (en) | 2011-06-24 | 2012-12-27 | Dr. Reddy's Laboratories Limited | Purification of chimeric protein |
| BR112014000352A2 (pt) * | 2011-07-08 | 2017-02-14 | Merck Sharp & Dohe Corp | método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada |
| CA2854233C (en) * | 2011-11-04 | 2020-05-12 | Zymeworks Inc. | Stable heterodimeric antibody design with mutations in the fc domain |
| SG10201701224UA (en) * | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| EP3395423B1 (en) * | 2013-03-14 | 2023-09-20 | Amgen Inc. | Removal of leaked affinity purification ligand |
-
2014
- 2014-03-11 EP EP18168494.5A patent/EP3395423B1/en active Active
- 2014-03-11 DK DK18168494.5T patent/DK3395423T3/da active
- 2014-03-11 MX MX2019006568A patent/MX391507B/es unknown
- 2014-03-11 SI SI201432052T patent/SI3395423T1/sl unknown
- 2014-03-11 PL PL18168494.5T patent/PL3395423T3/pl unknown
- 2014-03-11 PT PT181684945T patent/PT3395423T/pt unknown
- 2014-03-11 PL PL14714890T patent/PL2969099T5/pl unknown
- 2014-03-11 PT PT147148902T patent/PT2969099T/pt unknown
- 2014-03-11 LT LTEP18168494.5T patent/LT3395423T/lt unknown
- 2014-03-11 HU HUE18168494A patent/HUE064450T2/hu unknown
- 2014-03-11 EP EP14714890.2A patent/EP2969099B2/en active Active
- 2014-03-11 FI FIEP18168494.5T patent/FI3395423T3/fi active
- 2014-03-11 MX MX2015011994A patent/MX365501B/es active IP Right Grant
- 2014-03-11 US US14/775,992 patent/US20160024144A1/en not_active Abandoned
- 2014-03-11 JP JP2016501314A patent/JP6457479B2/ja active Active
- 2014-03-11 CA CA2904411A patent/CA2904411A1/en not_active Abandoned
- 2014-03-11 LT LTEP14714890.2T patent/LT2969099T/lt unknown
- 2014-03-11 HU HUE14714890A patent/HUE038565T2/hu unknown
- 2014-03-11 ES ES18168494T patent/ES2964601T3/es active Active
- 2014-03-11 ES ES14714890T patent/ES2674697T5/es active Active
- 2014-03-11 EP EP22155498.3A patent/EP4026596A1/en active Pending
- 2014-03-11 SI SI201430759T patent/SI2969099T2/sl unknown
- 2014-03-11 AU AU2014240596A patent/AU2014240596B2/en active Active
- 2014-03-11 DK DK14714890.2T patent/DK2969099T4/da active
- 2014-03-11 WO PCT/US2014/023682 patent/WO2014159441A1/en not_active Ceased
- 2014-03-11 TR TR2018/09050T patent/TR201809050T4/tr unknown
-
2018
- 2018-06-20 CY CY20181100641T patent/CY1120353T1/el unknown
- 2018-07-23 US US16/042,965 patent/US11192919B2/en active Active
-
2021
- 2021-12-17 US US17/554,865 patent/US11492372B2/en active Active
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