JP2015537212A5 - - Google Patents

Download PDF

Info

Publication number
JP2015537212A5
JP2015537212A5 JP2015542849A JP2015542849A JP2015537212A5 JP 2015537212 A5 JP2015537212 A5 JP 2015537212A5 JP 2015542849 A JP2015542849 A JP 2015542849A JP 2015542849 A JP2015542849 A JP 2015542849A JP 2015537212 A5 JP2015537212 A5 JP 2015537212A5
Authority
JP
Japan
Prior art keywords
gradient
polypeptide
contaminants
ionic strength
chromatography material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2015542849A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015537212A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2013/070415 external-priority patent/WO2014078729A1/en
Publication of JP2015537212A publication Critical patent/JP2015537212A/ja
Publication of JP2015537212A5 publication Critical patent/JP2015537212A5/ja
Pending legal-status Critical Current

Links

JP2015542849A 2012-11-15 2013-11-15 イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー Pending JP2015537212A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261727051P 2012-11-15 2012-11-15
US61/727,051 2012-11-15
US201361780707P 2013-03-13 2013-03-13
US61/780,707 2013-03-13
PCT/US2013/070415 WO2014078729A1 (en) 2012-11-15 2013-11-15 IONIC STRENGTH-MEDIATED pH GRADIENT ION EXCHANGE CHROMATOGRAPHY

Publications (2)

Publication Number Publication Date
JP2015537212A JP2015537212A (ja) 2015-12-24
JP2015537212A5 true JP2015537212A5 (https=) 2016-12-28

Family

ID=50731733

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015542849A Pending JP2015537212A (ja) 2012-11-15 2013-11-15 イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー

Country Status (11)

Country Link
US (2) US9810670B2 (https=)
EP (1) EP2920586A4 (https=)
JP (1) JP2015537212A (https=)
KR (1) KR20150084046A (https=)
CN (1) CN105051528A (https=)
BR (1) BR112015011011A2 (https=)
CA (1) CA2890979A1 (https=)
HK (1) HK1217232A1 (https=)
MX (1) MX2015006149A (https=)
RU (1) RU2015122726A (https=)
WO (1) WO2014078729A1 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105051528A (zh) 2012-11-15 2015-11-11 弗·哈夫曼-拉罗切有限公司 离子强度介导的pH梯度离子交换色谱
AU2014287044B2 (en) 2013-07-12 2020-02-06 Genentech, Inc. Elucidation of ion exchange chromatography input optimization
JP6962819B2 (ja) * 2015-04-10 2021-11-05 アディマブ， エルエルシー 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
BR112018009881A2 (pt) * 2015-11-18 2018-11-13 Merck Patent Gmbh separação de proteína aperfeiçoada em cromatografia de troca iônica
JP2018537458A (ja) * 2015-11-18 2018-12-20 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ 改善されたタンパク質分離のための逆ｐＨ塩勾配
CN108333264A (zh) * 2017-01-20 2018-07-27 湖北生物医药产业技术研究院有限公司 检测蛋白电荷变异体的方法及确定生物制品生产工艺的方法
US20190126241A1 (en) * 2017-09-26 2019-05-02 Waters Technologies Corporation High purity chromatographic materials comprising an ionizable modifier for retention of acidic analytes
WO2019217570A1 (en) 2018-05-08 2019-11-14 Waters Technologies Corporation Methods, compositions and kits useful for ph gradient cation exchange chromatography
KR20200080748A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
CN111024883B (zh) * 2019-12-31 2022-12-06 上海博威生物医药有限公司 一种奥马珠单抗的电荷异质体的分离方法
CN111239278B (zh) * 2020-02-05 2022-11-04 康立泰生物医药(青岛)有限公司 一种检测重组人白介素-12蛋白电荷变异体的方法及应用
US20230143066A1 (en) * 2020-04-17 2023-05-11 Ono Pharmaceutical Co., Ltd. Method for removing color from drug substance of protein preparation
KR102283871B1 (ko) * 2021-03-02 2021-08-02 한국원자력연구원 크로마토그래피를 이용한 무담체 홀뮴-166의 분리 방법, 분리 장치 및 이에 의하여 분리된 무담체 홀뮴-166
WO2023287821A1 (en) * 2021-07-13 2023-01-19 Regeneron Pharmaceuticals, Inc. Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms)
WO2023107510A1 (en) * 2021-12-06 2023-06-15 Staveness Daryl Exciplex-based methods for contaminant and disease detection and methods for use of extraction buffers
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
AU2023409836A1 (en) * 2022-12-20 2025-07-03 Seqirus Inc. Lipid nanoparticle composition
WO2024261724A1 (en) * 2023-06-23 2024-12-26 Lupin Limited Ion-exchange chromatography for separation and analysis of charge variants
AU2024323936A1 (en) * 2023-08-15 2026-04-02 Gigagen, Inc. Methods of analyzing recombinant polyclonal proteins

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US5567610A (en) 1986-09-04 1996-10-22 Bioinvent International Ab Method of producing human monoclonal antibodies and kit therefor
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
DE3850542T2 (de) 1987-09-23 1994-11-24 Bristol Myers Squibb Co Antikörper-Heterokonjugate zur Töting von HIV-infizierten Zellen.
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
ES2096590T3 (es) 1989-06-29 1997-03-16 Medarex Inc Reactivos biespecificos para la terapia del sida.
US5208020A (en) 1989-10-25 1993-05-04 Immunogen Inc. Cytotoxic agents comprising maytansinoids and their therapeutic use
US5356788A (en) * 1990-03-14 1994-10-18 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Isolation, quantitation and purification of insecticidal proteins from Bacillus thuringiensis
US5229275A (en) 1990-04-26 1993-07-20 Akzo N.V. In-vitro method for producing antigen-specific human monoclonal antibodies
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
WO1992020373A1 (en) 1991-05-14 1992-11-26 Repligen Corporation Heteroconjugate antibodies for treatment of hiv infection
EP1400536A1 (en) 1991-06-14 2004-03-24 Genentech Inc. Method for making humanized antibodies
MX9204374A (es) 1991-07-25 1993-03-01 Idec Pharma Corp Anticuerpo recombinante y metodo para su produccion.
US5565332A (en) 1991-09-23 1996-10-15 Medical Research Council Production of chimeric antibodies - a combinatorial approach
FI941572A7 (fi) 1991-10-07 1994-05-27 Oncologix Inc Anti-erbB-2-monoklonaalisten vasta-aineiden yhdistelmä ja käyttömenete lmä
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
ATE207080T1 (de) 1991-11-25 2001-11-15 Enzon Inc Multivalente antigen-bindende proteine
CA2372813A1 (en) 1992-02-06 1993-08-19 L.L. Houston Biosynthetic binding protein for cancer marker
WO1993016177A1 (en) 1992-02-11 1993-08-19 Cell Genesys, Inc. Homogenotization of gene-targeting events
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
CA2140280A1 (en) 1992-08-17 1994-03-03 Avi J. Ashkenazi Bispecific immunoadhesins
DK0752248T3 (da) 1992-11-13 2000-11-13 Idec Pharma Corp Terapeutisk anvendelse af kimæriske og radioaktivt mærkede antistoffer mod humant B-lymfocytbegrænset differentieringsantig
US5534615A (en) 1994-04-25 1996-07-09 Genentech, Inc. Cardiac hypertrophy factor and uses therefor
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5712374A (en) 1995-06-07 1998-01-27 American Cyanamid Company Method for the preparation of substantiallly monomeric calicheamicin derivative/carrier conjugates
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
EP2348044A1 (en) 1999-03-15 2011-07-27 Novo Nordisk A/S Ion exchange chromatography of GLP-1, analogs and derivatives thereof
AU2002226095A1 (en) * 2000-12-13 2002-06-24 Wackvom Limited Compositions containing an active fraction isolated from tricholoma conglobatum and methods of use
US8372954B2 (en) 2000-12-22 2013-02-12 National Research Council Of Canada Phage display libraries of human VH fragments
US20030031627A1 (en) * 2001-07-31 2003-02-13 Mallinckrodt Inc. Internal image antibodies for optical imaging and therapy
JP2005289809A (ja) 2001-10-24 2005-10-20 Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) 突然変異重鎖抗体
CA2516518A1 (en) * 2003-02-28 2004-09-10 Lonza Biologics Plc. Antibody purification by protein a and ion exchange chromatography
DE602004017726D1 (de) 2003-06-30 2008-12-24 Domantis Ltd Pegylierte Single-domain-antikörper (dAb)
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
CN101023101A (zh) * 2004-07-20 2007-08-22 西福根有限公司 抗－恒河猴d重组多克隆抗体和生产方法
AU2005285347A1 (en) 2004-08-19 2006-03-23 Genentech, Inc. Polypeptide variants with altered effector function
WO2006028936A2 (en) 2004-09-02 2006-03-16 Genentech, Inc. Heteromultimeric molecules
DK1791565T3 (en) 2004-09-23 2016-08-01 Genentech Inc Cysteingensplejsede antibodies and conjugates
US20060240564A1 (en) * 2005-04-20 2006-10-26 Chia-Hui Shieh pH gradient ion exchange LC-MS and mass compatible buffers
WO2007075283A2 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
CN100506983C (zh) * 2006-05-25 2009-07-01 齐鲁制药有限公司 一种从矛头蝮蛇毒中提取单一成份巴曲酶的方法
JP2010510963A (ja) 2006-06-14 2010-04-08 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー セラミックヒドロキシアパタイトを使用する抗体の精製方法
US20100330600A1 (en) * 2008-02-29 2010-12-30 Sekisui Medical Co., Ltd. High-molecular-weight adiponectin measurement method
US20120177640A1 (en) 2009-07-24 2012-07-12 Josef Burg Optimizing the production of antibodies
EP2480561B1 (en) 2009-09-23 2016-07-13 E. R. Squibb & Sons, L.L.C. Cation exchange chromatography
KR101574864B1 (ko) 2010-12-21 2015-12-11 에프. 호프만-라 로슈 아게 이소폼이 농축된 항체 제제 및 그의 제조 방법
AR085404A1 (es) 2011-02-28 2013-09-25 Hoffmann La Roche Proteinas de union a antigeno
WO2013122834A1 (en) * 2012-02-14 2013-08-22 Bio-Rad Laboratories, Inc. REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY
CN105051528A (zh) 2012-11-15 2015-11-11 弗·哈夫曼-拉罗切有限公司 离子强度介导的pH梯度离子交换色谱

Similar Documents

Publication Publication Date Title
JP2015537212A5 (https=)
RU2015122726A (ru) ОПОСРЕДОВАННАЯ ИОННОЙ СИЛОЙ рН-ГРАДИЕНТНАЯ ИОНООБМЕННАЯ ХРОМАТОГРАФИЯ
US10053489B2 (en) Method for purifying antibody
JP2018510867A5 (https=)
Maria et al. Purification process of recombinant monoclonal antibodies with mixed mode chromatography
US10246484B2 (en) Method for purifying recombinant protein
JP2017517745A5 (https=)
RU2016137932A (ru) In vitro прогнозирование времени полужизни антител in vivo
NZ714079A (en) Purification process for monoclonal antibodies
RU2016112549A (ru) Способ для повторного использования хроматографии
JP2016529489A5 (https=)
JP2016530533A5 (https=)
Chiverton et al. Quantitative definition and monitoring of the host cell protein proteome using iTRAQ–a study of an industrial mAb producing CHO‐S cell line
RU2018121657A (ru) ПРОТИВОПОЛОЖНЫЕ ГРАДИЕНТЫ pH-СОЛЬ ДЛЯ УЛУЧШЕННОГО РАЗДЕЛЕНИЯ БЕЛКОВ
RU2016104542A (ru) Осуществление оптимизации входных условий ионообменной хроматографии
RU2018121653A (ru) Улучшенное разделение белков при ионообменной хроматографии
JP2018516229A5 (https=)
US20170198008A1 (en) Methods and reagents for purification of proteins
KR20220079910A (ko) 숙주-세포 단백질을 특성화하는 방법
Yang et al. Effect of cleaning agents and additives on Protein A ligand degradation and chromatography performance
Scheffel et al. Optimization of a calcium-dependent Protein A-derived domain for mild antibody purification
Gjoka et al. Combined Protein A and size exclusion high performance liquid chromatography for the single-step measurement of mAb, aggregates and host cell proteins
Nadar et al. Design and optimization of membrane chromatography for monoclonal antibody charge variant separation
JP2016513643A (ja) 漏出したアフィニティ精製リガンドの除去
Marek et al. Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. I: Assessment of different separation concepts