RU2015122726A - ОПОСРЕДОВАННАЯ ИОННОЙ СИЛОЙ рН-ГРАДИЕНТНАЯ ИОНООБМЕННАЯ ХРОМАТОГРАФИЯ - Google Patents
ОПОСРЕДОВАННАЯ ИОННОЙ СИЛОЙ рН-ГРАДИЕНТНАЯ ИОНООБМЕННАЯ ХРОМАТОГРАФИЯ Download PDFInfo
- Publication number
- RU2015122726A RU2015122726A RU2015122726A RU2015122726A RU2015122726A RU 2015122726 A RU2015122726 A RU 2015122726A RU 2015122726 A RU2015122726 A RU 2015122726A RU 2015122726 A RU2015122726 A RU 2015122726A RU 2015122726 A RU2015122726 A RU 2015122726A
- Authority
- RU
- Russia
- Prior art keywords
- gradient
- polypeptide
- paragraphs
- antibody
- chromatographic material
- Prior art date
Links
- 238000004255 ion exchange chromatography Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims 90
- 229920001184 polypeptide Polymers 0.000 claims 50
- 102000004196 processed proteins & peptides Human genes 0.000 claims 50
- 108090000765 processed proteins & peptides Proteins 0.000 claims 50
- 239000012535 impurity Substances 0.000 claims 29
- 239000000463 material Substances 0.000 claims 29
- 239000000872 buffer Substances 0.000 claims 26
- 239000000203 mixture Substances 0.000 claims 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 12
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims 12
- 239000012149 elution buffer Substances 0.000 claims 11
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 claims 8
- 238000005342 ion exchange Methods 0.000 claims 8
- 239000012634 fragment Substances 0.000 claims 7
- 239000011780 sodium chloride Substances 0.000 claims 6
- 239000007983 Tris buffer Substances 0.000 claims 4
- 238000005349 anion exchange Methods 0.000 claims 4
- 238000005341 cation exchange Methods 0.000 claims 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 4
- 239000007857 degradation product Substances 0.000 claims 3
- 239000008001 CAPS buffer Substances 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 229910019142 PO4 Inorganic materials 0.000 claims 2
- 150000001412 amines Chemical group 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 150000002500 ions Chemical class 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 2
- 239000010452 phosphate Substances 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 150000003512 tertiary amines Chemical class 0.000 claims 2
- 230000015556 catabolic process Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 238000004128 high performance liquid chromatography Methods 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261727051P | 2012-11-15 | 2012-11-15 | |
| US61/727,051 | 2012-11-15 | ||
| US201361780707P | 2013-03-13 | 2013-03-13 | |
| US61/780,707 | 2013-03-13 | ||
| PCT/US2013/070415 WO2014078729A1 (en) | 2012-11-15 | 2013-11-15 | IONIC STRENGTH-MEDIATED pH GRADIENT ION EXCHANGE CHROMATOGRAPHY |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2015122726A true RU2015122726A (ru) | 2017-01-10 |
Family
ID=50731733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015122726A RU2015122726A (ru) | 2012-11-15 | 2013-11-15 | ОПОСРЕДОВАННАЯ ИОННОЙ СИЛОЙ рН-ГРАДИЕНТНАЯ ИОНООБМЕННАЯ ХРОМАТОГРАФИЯ |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US9810670B2 (https=) |
| EP (1) | EP2920586A4 (https=) |
| JP (1) | JP2015537212A (https=) |
| KR (1) | KR20150084046A (https=) |
| CN (1) | CN105051528A (https=) |
| BR (1) | BR112015011011A2 (https=) |
| CA (1) | CA2890979A1 (https=) |
| HK (1) | HK1217232A1 (https=) |
| MX (1) | MX2015006149A (https=) |
| RU (1) | RU2015122726A (https=) |
| WO (1) | WO2014078729A1 (https=) |
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| CN105051528A (zh) | 2012-11-15 | 2015-11-11 | 弗·哈夫曼-拉罗切有限公司 | 离子强度介导的pH梯度离子交换色谱 |
| AU2014287044B2 (en) | 2013-07-12 | 2020-02-06 | Genentech, Inc. | Elucidation of ion exchange chromatography input optimization |
| JP6962819B2 (ja) * | 2015-04-10 | 2021-11-05 | アディマブ， エルエルシー | 親ホモ二量体抗体種からのヘテロ二量体多重特異性抗体の精製方法 |
| GB2539420B (en) * | 2015-06-16 | 2021-01-13 | Cytiva Sweden Ab | Determination of chromatography conditions |
| BR112018009881A2 (pt) * | 2015-11-18 | 2018-11-13 | Merck Patent Gmbh | separação de proteína aperfeiçoada em cromatografia de troca iônica |
| JP2018537458A (ja) * | 2015-11-18 | 2018-12-20 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングＭｅｒｃｋ Ｐａｔｅｎｔ Ｇｅｓｅｌｌｓｃｈａｆｔ ｍｉｔ ｂｅｓｃｈｒａｅｎｋｔｅｒ Ｈａｆｔｕｎｇ | 改善されたタンパク質分離のための逆ｐＨ塩勾配 |
| CN108333264A (zh) * | 2017-01-20 | 2018-07-27 | 湖北生物医药产业技术研究院有限公司 | 检测蛋白电荷变异体的方法及确定生物制品生产工艺的方法 |
| US20190126241A1 (en) * | 2017-09-26 | 2019-05-02 | Waters Technologies Corporation | High purity chromatographic materials comprising an ionizable modifier for retention of acidic analytes |
| WO2019217570A1 (en) | 2018-05-08 | 2019-11-14 | Waters Technologies Corporation | Methods, compositions and kits useful for ph gradient cation exchange chromatography |
| KR20200080748A (ko) * | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법 |
| TW202128264A (zh) * | 2019-09-25 | 2021-08-01 | 瑞士商赫孚孟拉羅股份公司 | 使用經預測之溶離緩衝鹽濃度的陽離子層析法 |
| CN111024883B (zh) * | 2019-12-31 | 2022-12-06 | 上海博威生物医药有限公司 | 一种奥马珠单抗的电荷异质体的分离方法 |
| CN111239278B (zh) * | 2020-02-05 | 2022-11-04 | 康立泰生物医药(青岛)有限公司 | 一种检测重组人白介素-12蛋白电荷变异体的方法及应用 |
| US20230143066A1 (en) * | 2020-04-17 | 2023-05-11 | Ono Pharmaceutical Co., Ltd. | Method for removing color from drug substance of protein preparation |
| KR102283871B1 (ko) * | 2021-03-02 | 2021-08-02 | 한국원자력연구원 | 크로마토그래피를 이용한 무담체 홀뮴-166의 분리 방법, 분리 장치 및 이에 의하여 분리된 무담체 홀뮴-166 |
| WO2023287821A1 (en) * | 2021-07-13 | 2023-01-19 | Regeneron Pharmaceuticals, Inc. | Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms) |
| WO2023107510A1 (en) * | 2021-12-06 | 2023-06-15 | Staveness Daryl | Exciplex-based methods for contaminant and disease detection and methods for use of extraction buffers |
| CN114544839B (zh) * | 2022-01-20 | 2024-08-20 | 未名生物医药有限公司 | 一种抗人神经生长因子抗体的电荷变异体检测方法 |
| AU2023409836A1 (en) * | 2022-12-20 | 2025-07-03 | Seqirus Inc. | Lipid nanoparticle composition |
| WO2024261724A1 (en) * | 2023-06-23 | 2024-12-26 | Lupin Limited | Ion-exchange chromatography for separation and analysis of charge variants |
| AU2024323936A1 (en) * | 2023-08-15 | 2026-04-02 | Gigagen, Inc. | Methods of analyzing recombinant polyclonal proteins |
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-
2013
- 2013-11-15 CN CN201380070366.XA patent/CN105051528A/zh active Pending
- 2013-11-15 BR BR112015011011A patent/BR112015011011A2/pt not_active Application Discontinuation
- 2013-11-15 JP JP2015542849A patent/JP2015537212A/ja active Pending
- 2013-11-15 US US14/443,329 patent/US9810670B2/en active Active
- 2013-11-15 MX MX2015006149A patent/MX2015006149A/es unknown
- 2013-11-15 WO PCT/US2013/070415 patent/WO2014078729A1/en not_active Ceased
- 2013-11-15 CA CA2890979A patent/CA2890979A1/en not_active Abandoned
- 2013-11-15 KR KR1020157015377A patent/KR20150084046A/ko not_active Withdrawn
- 2013-11-15 RU RU2015122726A patent/RU2015122726A/ru not_active Application Discontinuation
- 2013-11-15 HK HK16105250.6A patent/HK1217232A1/zh unknown
- 2013-11-15 EP EP13855528.9A patent/EP2920586A4/en not_active Withdrawn
-
2017
- 2017-09-29 US US15/721,389 patent/US10712322B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2890979A1 (en) | 2014-05-22 |
| EP2920586A1 (en) | 2015-09-23 |
| US10712322B2 (en) | 2020-07-14 |
| US20180120273A1 (en) | 2018-05-03 |
| US20150285771A1 (en) | 2015-10-08 |
| CN105051528A (zh) | 2015-11-11 |
| EP2920586A4 (en) | 2017-01-04 |
| MX2015006149A (es) | 2015-08-05 |
| KR20150084046A (ko) | 2015-07-21 |
| BR112015011011A2 (pt) | 2019-12-17 |
| US9810670B2 (en) | 2017-11-07 |
| WO2014078729A1 (en) | 2014-05-22 |
| HK1217232A1 (zh) | 2016-12-30 |
| JP2015537212A (ja) | 2015-12-24 |
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