JP2013511566A5 - - Google Patents

Info

Publication number

JP2013511566A5

JP2013511566A5 JP2012540414A JP2012540414A JP2013511566A5 JP 2013511566 A5 JP2013511566 A5 JP 2013511566A5 JP 2012540414 A JP2012540414 A JP 2012540414A JP 2012540414 A JP2012540414 A JP 2012540414A JP 2013511566 A5 JP2013511566 A5 JP 2013511566A5

Authority

JP

Japan

Prior art keywords

protein

factor

pegylated protein

buffer

purified

Prior art date

2010-11-24

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
• Discuss

• 238000000034 method Methods 0.000 claims 11
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 6
• 108091006006 PEGylated Proteins Proteins 0.000 claims 6
• 230000002378 acidificating effect Effects 0.000 claims 4
• 239000012535 impurity Substances 0.000 claims 4
• 102100022641 Coagulation factor IX Human genes 0.000 claims 3
• 108010076282 Factor IX Proteins 0.000 claims 3
• 239000000872 buffer Substances 0.000 claims 3
• 229960004222 factor ix Drugs 0.000 claims 3
• 239000008194 pharmaceutical composition Substances 0.000 claims 3
• 238000005406 washing Methods 0.000 claims 3
• 102100023804 Coagulation factor VII Human genes 0.000 claims 2
• 108010023321 Factor VII Proteins 0.000 claims 2
• 108010014173 Factor X Proteins 0.000 claims 2
• 108010094028 Prothrombin Proteins 0.000 claims 2
• 238000005349 anion exchange Methods 0.000 claims 2
• AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 claims 2
• 238000011067 equilibration Methods 0.000 claims 2
• 239000006167 equilibration buffer Substances 0.000 claims 2
• 229940012413 factor vii Drugs 0.000 claims 2
• 208000031220 Hemophilia Diseases 0.000 claims 1
• 208000009292 Hemophilia A Diseases 0.000 claims 1
• 102000017975 Protein C Human genes 0.000 claims 1
• 229940096437 Protein S Drugs 0.000 claims 1
• 102000029301 Protein S Human genes 0.000 claims 1
• 108010066124 Protein S Proteins 0.000 claims 1
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 1
• BDKZHNJTLHOSDW-UHFFFAOYSA-N [Na].CC(O)=O Chemical compound [Na].CC(O)=O BDKZHNJTLHOSDW-UHFFFAOYSA-N 0.000 claims 1
• 238000005571 anion exchange chromatography Methods 0.000 claims 1
• 238000004587 chromatography analysis Methods 0.000 claims 1
• 238000010828 elution Methods 0.000 claims 1
• 239000012149 elution buffer Substances 0.000 claims 1
• 239000000463 material Substances 0.000 claims 1
• 229960000856 protein c Drugs 0.000 claims 1
• 238000007670 refining Methods 0.000 claims 1
• 239000001632 sodium acetate Substances 0.000 claims 1
• 235000017281 sodium acetate Nutrition 0.000 claims 1