JP2013503877A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013503877A5 JP2013503877A5 JP2012527990A JP2012527990A JP2013503877A5 JP 2013503877 A5 JP2013503877 A5 JP 2013503877A5 JP 2012527990 A JP2012527990 A JP 2012527990A JP 2012527990 A JP2012527990 A JP 2012527990A JP 2013503877 A5 JP2013503877 A5 JP 2013503877A5
- Authority
- JP
- Japan
- Prior art keywords
- polypeptide
- buffer
- low
- elution
- separated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 26
- 229920001184 polypeptide Polymers 0.000 claims 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims 20
- 108090000765 processed proteins & peptides Proteins 0.000 claims 20
- 239000006174 pH buffer Substances 0.000 claims 11
- 239000012149 elution buffer Substances 0.000 claims 6
- 239000012560 cell impurity Substances 0.000 claims 4
- 238000010828 elution Methods 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 3
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims 3
- 239000000178 monomer Substances 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23886709P | 2009-09-01 | 2009-09-01 | |
| US61/238,867 | 2009-09-01 | ||
| US25343809P | 2009-10-20 | 2009-10-20 | |
| US61/253,438 | 2009-10-20 | ||
| PCT/US2010/047448 WO2011028753A1 (en) | 2009-09-01 | 2010-09-01 | Enhanced protein purification through a modified protein a elution |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013503877A JP2013503877A (ja) | 2013-02-04 |
| JP2013503877A5 true JP2013503877A5 (https=) | 2013-10-10 |
| JP5787891B2 JP5787891B2 (ja) | 2015-09-30 |
Family
ID=43649614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012527990A Active JP5787891B2 (ja) | 2009-09-01 | 2010-09-01 | 改変されたプロテインａ溶離による向上したタンパク質精製 |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US9428548B2 (https=) |
| EP (2) | EP2473617B1 (https=) |
| JP (1) | JP5787891B2 (https=) |
| KR (1) | KR101764449B1 (https=) |
| CN (2) | CN108409829A (https=) |
| BR (1) | BR112012004697B8 (https=) |
| CA (1) | CA2772653C (https=) |
| ES (1) | ES2787234T3 (https=) |
| HR (1) | HRP20200581T1 (https=) |
| MX (1) | MX2012002565A (https=) |
| PL (1) | PL2473617T3 (https=) |
| RU (1) | RU2571929C2 (https=) |
| SI (1) | SI2473617T1 (https=) |
| WO (1) | WO2011028753A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| TWI456062B (zh) | 2006-09-13 | 2014-10-11 | Abbvie Inc | 細胞培養改良 |
| MX2011004201A (es) | 2008-10-20 | 2011-05-24 | Abbott Lab | Desactivacion viral durante purificacion de anticuerpos. |
| AU2009347206C1 (en) | 2008-10-20 | 2016-12-08 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
| MX346115B (es) | 2009-08-06 | 2017-03-08 | Genentech Inc * | Metodo para mejorar la eliminación de virus en la purificacion proteica. |
| EP2475678B1 (en) * | 2009-09-10 | 2014-10-08 | Lonza Biologics Plc. | Method and system for polypeptide purification |
| RU2648999C2 (ru) * | 2011-12-22 | 2018-03-29 | Дженентек, Инк. | Способы повышения эффективности этапов очистки белка, находящихся ниже по потоку, с использованием мембранной ионообменной хроматографии |
| US10189891B2 (en) | 2012-03-28 | 2019-01-29 | Ge Healthcare Bioprocess R&D Ab | Affinity chromatography matrix |
| CN104411820B (zh) | 2012-06-29 | 2017-05-03 | Emd密理博公司 | 在蛋白纯化过程中灭活病毒的方法 |
| US8921113B2 (en) | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| EP2970379B1 (en) * | 2013-03-14 | 2019-05-01 | EMD Millipore Corporation | Methods of increasing protein purity using protein a based chromatography |
| NO2994485T3 (https=) * | 2013-05-06 | 2018-06-09 | ||
| AU2014287044B2 (en) * | 2013-07-12 | 2020-02-06 | Genentech, Inc. | Elucidation of ion exchange chromatography input optimization |
| MX380902B (es) * | 2013-11-15 | 2025-03-12 | Genentech Inc | Métodos para la inactivación viral usando detergentes ecológico. |
| ES2784749T3 (es) * | 2014-03-10 | 2020-09-30 | Richter Gedeon Nyrt | Purificación de inmunoglobulina con el uso de etapas de limpieza previa |
| GB2539420B (en) * | 2015-06-16 | 2021-01-13 | Cytiva Sweden Ab | Determination of chromatography conditions |
| TWI848953B (zh) | 2018-06-09 | 2024-07-21 | 德商百靈佳殷格翰國際股份有限公司 | 針對癌症治療之多特異性結合蛋白 |
| CN112566930A (zh) * | 2018-06-19 | 2021-03-26 | 百时美施贵宝公司 | 使用色谱纯化蛋白质的方法 |
| US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| CA3167669A1 (en) * | 2020-01-17 | 2021-07-22 | Regeneron Pharmaceuticals, Inc. | Hydrophobic interaction chromatography for viral clearance |
| KR20250011797A (ko) | 2023-07-13 | 2025-01-22 | 주식회사 녹십자 | 알부민이 융합된 단백질의 정제방법 |
Family Cites Families (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3773919A (en) | 1969-10-23 | 1973-11-20 | Du Pont | Polylactide-drug mixtures |
| USRE30985E (en) | 1978-01-01 | 1982-06-29 | Serum-free cell culture media | |
| FR2413974A1 (fr) | 1978-01-06 | 1979-08-03 | David Bernard | Sechoir pour feuilles imprimees par serigraphie |
| US4515893A (en) | 1979-04-26 | 1985-05-07 | Ortho Pharmaceutical Corporation | Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells |
| US4560655A (en) | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| DD266710A3 (de) | 1983-06-06 | 1989-04-12 | Ve Forschungszentrum Biotechnologie | Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase |
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US5672347A (en) | 1984-07-05 | 1997-09-30 | Genentech, Inc. | Tumor necrosis factor antagonists and their use |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| GB8516415D0 (en) | 1985-06-28 | 1985-07-31 | Celltech Ltd | Culture of animal cells |
| US4676980A (en) | 1985-09-23 | 1987-06-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target specific cross-linked heteroantibodies |
| US5091178A (en) | 1986-02-21 | 1992-02-25 | Oncogen | Tumor therapy with biologically active anti-tumor antibodies |
| US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| DE3850542T2 (de) | 1987-09-23 | 1994-11-24 | Bristol Myers Squibb Co | Antikörper-Heterokonjugate zur Töting von HIV-infizierten Zellen. |
| US5091313A (en) | 1988-08-05 | 1992-02-25 | Tanox Biosystems, Inc. | Antigenic epitopes of IgE present on B cell but not basophil surface |
| US5720937A (en) | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
| EP0420937B1 (en) | 1988-06-21 | 1994-11-09 | Genentech, Inc. | Therapeutic compositions for the treatment of myocardial infarction |
| AU632065B2 (en) | 1988-09-23 | 1992-12-17 | Novartis Vaccines And Diagnostics, Inc. | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| EP0402226A1 (en) | 1989-06-06 | 1990-12-12 | Institut National De La Recherche Agronomique | Transformation vectors for yeast yarrowia |
| ES2096590T3 (es) | 1989-06-29 | 1997-03-16 | Medarex Inc | Reactivos biespecificos para la terapia del sida. |
| DE69023865T2 (de) | 1989-07-28 | 1996-10-17 | Wako Pure Chem Ind Ltd | Fulgimidderivate. |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| EP0586505A1 (en) | 1991-05-14 | 1994-03-16 | Repligen Corporation | Heteroconjugate antibodies for treatment of hiv infection |
| CA2113813C (en) | 1991-08-14 | 2005-04-12 | Paula M. Jardieu | Immunoglobulin variants for specific fc epsilon receptors |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| AU675929B2 (en) | 1992-02-06 | 1997-02-27 | Curis, Inc. | Biosynthetic binding protein for cancer marker |
| WO1994004188A1 (en) | 1992-08-21 | 1994-03-03 | Genentech, Inc. | Method for treating an lfa-1-mediated disorder |
| US5736137A (en) | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| DK0733207T3 (da) | 1993-12-10 | 1998-04-20 | Genentech Inc | Fremgangsmåder til diagnose af allergi og screening af anti-allergi-terapeutika |
| DK0739214T3 (da) | 1994-01-18 | 1998-10-07 | Genentech Inc | Fremgangsmåde til behandling af parasitinfektionerunder anvendelse af IgE antagonister |
| US5702946A (en) | 1994-03-03 | 1997-12-30 | Genentech, Inc. | Anti-IL-8 monoclonal antibodies for treatment of inflammatory disorders |
| US5534615A (en) | 1994-04-25 | 1996-07-09 | Genentech, Inc. | Cardiac hypertrophy factor and uses therefor |
| US5731168A (en) | 1995-03-01 | 1998-03-24 | Genentech, Inc. | Method for making heteromultimeric polypeptides |
| IL117645A (en) | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| AU6267896A (en) | 1995-06-07 | 1996-12-30 | Imclone Systems Incorporated | Antibody and antibody fragments for inhibiting the growth oftumors |
| IL125255A (en) | 1996-01-23 | 2002-02-10 | Genentech Inc | Anti-cd18 antibodies for treating stroke |
| US7147851B1 (en) | 1996-08-15 | 2006-12-12 | Millennium Pharmaceuticals, Inc. | Humanized immunoglobulin reactive with α4β7 integrin |
| WO1998023761A1 (en) | 1996-11-27 | 1998-06-04 | Genentech, Inc. | HUMANIZED ANTI-CD11a ANTIBODIES |
| US20030109680A1 (en) * | 2001-11-21 | 2003-06-12 | Sunol Molecular Corporation | Antibodies for inhibiting blood coagulation and methods of use thereof |
| JP3957765B2 (ja) | 1997-04-07 | 2007-08-15 | ジェネンテク・インコーポレイテッド | 抗ｖｅｇｆ抗体 |
| EP0981618B2 (en) | 1997-05-15 | 2011-08-24 | Genentech, Inc. | Anti-apo-2 antibody |
| US5994511A (en) | 1997-07-02 | 1999-11-30 | Genentech, Inc. | Anti-IgE antibodies and methods of improving polypeptides |
| RU2145610C1 (ru) | 1998-12-09 | 2000-02-20 | Общество с ограниченной ответственностью "Протеиновый контур" | Способ очистки рекомбинантного эритропоэтина человека |
| US6946129B1 (en) | 1999-06-08 | 2005-09-20 | Seattle Genetics, Inc. | Recombinant anti-CD40 antibody and uses thereof |
| CA2376596C (en) | 1999-06-25 | 2009-10-06 | Genentech, Inc. | Humanized anti-erbb2 antibodies and treatment with anti-erbb2 antibodies |
| AU778199B2 (en) | 1999-10-29 | 2004-11-25 | Genentech Inc. | Anti-prostate stem cell antigen (PSCA) antibody compositions and methods of use |
| DK1578774T3 (da) * | 2002-09-18 | 2014-02-03 | Danisco Us Inc | Oprensning af immunoglobuliner |
| CN101120017A (zh) * | 2004-08-30 | 2008-02-06 | 英国龙沙生物医药股份有限公司 | 用于纯化抗体的亲和力加离子交换层析 |
| EP1693108A1 (en) * | 2004-12-04 | 2006-08-23 | MERCK PATENT GmbH | Mixed-modal anion-exchange type separation material |
| TWI372763B (en) * | 2005-06-17 | 2012-09-21 | Wyeth Llc | Methods of purifying anti a beta antibodies |
| EA017733B1 (ru) * | 2006-08-28 | 2013-02-28 | Арес Трейдинг С.А. | СПОСОБ ОЧИСТКИ Fc-СЛИТЫХ БЕЛКОВ |
| WO2008085988A1 (en) * | 2007-01-05 | 2008-07-17 | Amgen Inc. | Methods of purifying proteins |
| NZ577933A (en) | 2007-01-22 | 2011-12-22 | Genentech Inc | Polyelectrolyte precipitation and purification of antibodies |
| AU2009347206C1 (en) * | 2008-10-20 | 2016-12-08 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
| JP5816170B2 (ja) * | 2009-06-26 | 2015-11-18 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | 天然の免疫グロブリン形式を有する容易に単離される二重特異性抗体 |
| WO2015198451A1 (ja) | 2014-06-26 | 2015-12-30 | 楽天株式会社 | 情報処理装置、情報処理方法及び情報処理プログラム |
-
2010
- 2010-09-01 JP JP2012527990A patent/JP5787891B2/ja active Active
- 2010-09-01 HR HRP20200581TT patent/HRP20200581T1/hr unknown
- 2010-09-01 CA CA2772653A patent/CA2772653C/en active Active
- 2010-09-01 BR BR112012004697A patent/BR112012004697B8/pt active IP Right Grant
- 2010-09-01 RU RU2012112536/10A patent/RU2571929C2/ru active
- 2010-09-01 ES ES10814394T patent/ES2787234T3/es active Active
- 2010-09-01 WO PCT/US2010/047448 patent/WO2011028753A1/en not_active Ceased
- 2010-09-01 MX MX2012002565A patent/MX2012002565A/es active IP Right Grant
- 2010-09-01 US US13/393,525 patent/US9428548B2/en active Active
- 2010-09-01 SI SI201032003T patent/SI2473617T1/sl unknown
- 2010-09-01 PL PL10814394T patent/PL2473617T3/pl unknown
- 2010-09-01 EP EP10814394.2A patent/EP2473617B1/en not_active Revoked
- 2010-09-01 EP EP20159179.9A patent/EP3736338A1/en not_active Withdrawn
- 2010-09-01 KR KR1020127008252A patent/KR101764449B1/ko active Active
- 2010-09-01 CN CN201810100462.9A patent/CN108409829A/zh active Pending
- 2010-09-01 CN CN2010800389895A patent/CN102686738A/zh active Pending
-
2021
- 2021-05-27 US US17/332,907 patent/US20220081466A1/en not_active Abandoned
- 2021-12-21 US US17/558,406 patent/US20220324905A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2013503877A5 (https=) | ||
| HRP20200581T1 (hr) | Pojačano pročišćavanje proteina kroz eluiranje modificiranog proteina a | |
| JP2016026215A5 (https=) | ||
| ES2784749T3 (es) | Purificación de inmunoglobulina con el uso de etapas de limpieza previa | |
| EP3004163B1 (en) | Method for purifying antibody | |
| JP2022036971A5 (https=) | ||
| HRP20210777T1 (hr) | Pripravci protiv cgrp-a njihova upotreba | |
| WO2009135656A1 (en) | A method for the purification of antibodies using displacement chromatography | |
| JP6456376B2 (ja) | 組換えタンパク質の精製方法 | |
| JP2018085988A5 (https=) | ||
| JP2011530592A5 (https=) | ||
| JP2016539176A5 (https=) | ||
| JP2014506790A5 (https=) | ||
| JP2010227116A5 (https=) | ||
| JP2016530533A5 (https=) | ||
| JP2011515073A5 (https=) | ||
| JP2005120106A5 (https=) | ||
| JP2016519137A5 (https=) | ||
| RU2016108828A (ru) | Способы и композиции, включающие очищенные рекомбинантные полипептиды | |
| JP7037535B2 (ja) | 産生細胞株エンハンサー | |
| JP2013511566A5 (https=) | ||
| JP2013511481A5 (https=) | ||
| CN103189390A (zh) | 单一单元离子交换色谱抗体纯化 | |
| WO2018018011A3 (en) | Methods of purifying fc-containing proteins | |
| Fan et al. | Directing membrane chromatography to manufacture α1-antitrypsin from human plasma fraction IV |