JP2011510303A5 - - Google Patents
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- Publication number
- JP2011510303A5 JP2011510303A5 JP2010543281A JP2010543281A JP2011510303A5 JP 2011510303 A5 JP2011510303 A5 JP 2011510303A5 JP 2010543281 A JP2010543281 A JP 2010543281A JP 2010543281 A JP2010543281 A JP 2010543281A JP 2011510303 A5 JP2011510303 A5 JP 2011510303A5
- Authority
- JP
- Japan
- Prior art keywords
- biomolecule
- borate
- apatite chromatography
- ionic species
- chromatography support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims description 65
- 229910052586 apatite Inorganic materials 0.000 claims description 29
- 238000004587 chromatography analysis Methods 0.000 claims description 29
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 claims description 29
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims description 28
- 241000894007 species Species 0.000 claims description 22
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 14
- 239000011575 calcium Substances 0.000 claims description 14
- 229910052791 calcium Inorganic materials 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 13
- 150000002500 ions Chemical class 0.000 claims description 11
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 10
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 10
- 238000010828 elution Methods 0.000 claims description 10
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 8
- 230000003139 buffering effect Effects 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 229910021538 borax Inorganic materials 0.000 claims description 4
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 4
- WUUHFRRPHJEEKV-UHFFFAOYSA-N tripotassium borate Chemical compound [K+].[K+].[K+].[O-]B([O-])[O-] WUUHFRRPHJEEKV-UHFFFAOYSA-N 0.000 claims description 4
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 claims description 4
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical group C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 claims description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
- 239000004475 Arginine Substances 0.000 claims description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims description 2
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 claims description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004472 Lysine Substances 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 2
- 241000700605 Viruses Species 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 239000004202 carbamide Substances 0.000 claims description 2
- 229910052587 fluorapatite Inorganic materials 0.000 claims description 2
- 229940050410 gluconate Drugs 0.000 claims description 2
- 229940097042 glucuronate Drugs 0.000 claims description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 2
- 229940001447 lactate Drugs 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 229920000620 organic polymer Polymers 0.000 claims description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical group [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 102000040430 polynucleotide Human genes 0.000 claims description 2
- 108091033319 polynucleotide Proteins 0.000 claims description 2
- 239000002157 polynucleotide Substances 0.000 claims description 2
- PHZLMBHDXVLRIX-UHFFFAOYSA-M potassium lactate Chemical compound [K+].CC(O)C([O-])=O PHZLMBHDXVLRIX-UHFFFAOYSA-M 0.000 claims description 2
- 239000001521 potassium lactate Substances 0.000 claims description 2
- 235000011085 potassium lactate Nutrition 0.000 claims description 2
- 229960001304 potassium lactate Drugs 0.000 claims description 2
- 238000000746 purification Methods 0.000 claims description 2
- 229940076788 pyruvate Drugs 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000001540 sodium lactate Substances 0.000 claims description 2
- 229940005581 sodium lactate Drugs 0.000 claims description 2
- 235000011088 sodium lactate Nutrition 0.000 claims description 2
- 229960005486 vaccine Drugs 0.000 claims description 2
Applications Claiming Priority (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1151308P | 2008-01-18 | 2008-01-18 | |
| US61/011,513 | 2008-01-18 | ||
| US6266308P | 2008-01-28 | 2008-01-28 | |
| US61/062,663 | 2008-01-28 | ||
| US6985908P | 2008-03-19 | 2008-03-19 | |
| US61/069,859 | 2008-03-19 | ||
| US7084108P | 2008-03-27 | 2008-03-27 | |
| US61/070,841 | 2008-03-27 | ||
| US13578708P | 2008-07-24 | 2008-07-24 | |
| US61/135,787 | 2008-07-24 | ||
| US18946708P | 2008-08-20 | 2008-08-20 | |
| US61/189,467 | 2008-08-20 | ||
| PCT/US2009/031293 WO2009092010A1 (en) | 2008-01-18 | 2009-01-16 | Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011510303A JP2011510303A (ja) | 2011-03-31 |
| JP2011510303A5 true JP2011510303A5 (https=) | 2012-03-01 |
| JP5624476B2 JP5624476B2 (ja) | 2014-11-12 |
Family
ID=40876787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010543281A Active JP5624476B2 (ja) | 2008-01-18 | 2009-01-16 | アパタイトクロマトグラフィーによるリン酸化生体分子および非リン酸化生体分子の向上した精製法 |
Country Status (4)
| Country | Link |
|---|---|
| US (5) | US20090186396A1 (https=) |
| EP (4) | EP2238154B1 (https=) |
| JP (1) | JP5624476B2 (https=) |
| WO (2) | WO2009092010A1 (https=) |
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| JP4160652B2 (ja) * | 1998-05-22 | 2008-10-01 | キリンファーマ株式会社 | エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子 |
| ES2322945T5 (es) | 2001-12-21 | 2012-10-17 | Immunex Corporation | Métodos para purficación de proteína |
| DE102004027816A1 (de) * | 2004-06-08 | 2006-01-05 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von Erythropoietin |
| WO2006017491A2 (en) * | 2004-08-04 | 2006-02-16 | Allos Therapeutics, Inc. | Methods and compositions to reduce tissue irritation in parenteral formulations |
| DE102004044419B4 (de) * | 2004-09-14 | 2010-04-15 | Biotest Ag | Verfahren zur Aufreinigung eines von Willebrand Faktors mittels Hydroxylapatit-Durchlaufchromatographie |
| EP1869065B1 (en) | 2005-03-11 | 2020-05-06 | Wyeth LLC | A method of weak partitioning chromatography |
| JP2008538181A (ja) * | 2005-03-30 | 2008-10-16 | ネオス テクノロジーズ インコーポレイテッド | 昆虫細胞系において増殖させたペプチドを生産するための製造方法 |
| JP2010510963A (ja) * | 2006-06-14 | 2010-04-08 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー | セラミックヒドロキシアパタイトを使用する抗体の精製方法 |
| US7999085B2 (en) | 2007-01-09 | 2011-08-16 | Bio-Rad Laboratories, Inc. | Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers |
| US7691980B2 (en) | 2007-01-09 | 2010-04-06 | Bio-Rad Laboratories, Inc. | Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers |
| EP2238154B1 (en) * | 2008-01-18 | 2015-09-16 | Bio-Rad Laboratories, Inc. | Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography |
-
2009
- 2009-01-16 EP EP09702238.8A patent/EP2238154B1/en not_active Not-in-force
- 2009-01-16 EP EP10178856.0A patent/EP2287174B8/en not_active Not-in-force
- 2009-01-16 WO PCT/US2009/031293 patent/WO2009092010A1/en not_active Ceased
- 2009-01-16 WO PCT/US2009/031298 patent/WO2009092014A1/en not_active Ceased
- 2009-01-16 EP EP20100178843 patent/EP2275431A3/en not_active Withdrawn
- 2009-01-16 EP EP09701572.1A patent/EP2242762B1/en not_active Not-in-force
- 2009-01-16 JP JP2010543281A patent/JP5624476B2/ja active Active
- 2009-01-16 US US12/355,686 patent/US20090186396A1/en not_active Abandoned
- 2009-01-16 US US12/355,665 patent/US8093364B2/en not_active Expired - Fee Related
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2011
- 2011-12-02 US US13/310,576 patent/US9127042B2/en active Active
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2015
- 2015-08-05 US US14/819,334 patent/US9938317B2/en not_active Expired - Fee Related
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2018
- 2018-03-02 US US15/910,702 patent/US10919930B2/en not_active Expired - Fee Related
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