JP2013517284A5 - - Google Patents
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- Publication number
- JP2013517284A5 JP2013517284A5 JP2012549083A JP2012549083A JP2013517284A5 JP 2013517284 A5 JP2013517284 A5 JP 2013517284A5 JP 2012549083 A JP2012549083 A JP 2012549083A JP 2012549083 A JP2012549083 A JP 2012549083A JP 2013517284 A5 JP2013517284 A5 JP 2013517284A5
- Authority
- JP
- Japan
- Prior art keywords
- target molecule
- solution
- apatite
- solid surface
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 45
- 239000007787 solid Substances 0.000 claims description 22
- -1 amino compound Chemical class 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 18
- 229910052586 apatite Inorganic materials 0.000 claims description 15
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000000919 ceramic Substances 0.000 claims description 9
- 229910001413 alkali metal ion Inorganic materials 0.000 claims description 8
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 6
- 229910052587 fluorapatite Inorganic materials 0.000 claims description 5
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 5
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000004475 Arginine Substances 0.000 claims description 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 3
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004472 Lysine Substances 0.000 claims description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims description 2
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 claims description 2
- 239000007991 ACES buffer Substances 0.000 claims description 2
- 239000007995 HEPES buffer Substances 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 2
- 239000007987 MES buffer Substances 0.000 claims description 2
- 239000007993 MOPS buffer Substances 0.000 claims description 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims description 2
- 239000007990 PIPES buffer Substances 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 239000002158 endotoxin Substances 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229910052744 lithium Inorganic materials 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 150000003384 small molecules Chemical class 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29549910P | 2010-01-15 | 2010-01-15 | |
| US61/295,499 | 2010-01-15 | ||
| PCT/US2011/021158 WO2011088225A1 (en) | 2010-01-15 | 2011-01-13 | Surface neutralization of apatite |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013517284A JP2013517284A (ja) | 2013-05-16 |
| JP2013517284A5 true JP2013517284A5 (https=) | 2014-01-30 |
| JP5773454B2 JP5773454B2 (ja) | 2015-09-02 |
Family
ID=44278020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012549083A Active JP5773454B2 (ja) | 2010-01-15 | 2011-01-13 | アパタイトの表面中和方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US8951807B2 (https=) |
| EP (1) | EP2524215B1 (https=) |
| JP (1) | JP5773454B2 (https=) |
| CA (1) | CA2787009C (https=) |
| WO (1) | WO2011088225A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011088225A1 (en) * | 2010-01-15 | 2011-07-21 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| JP5881744B2 (ja) * | 2011-02-02 | 2016-03-09 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | アルカリ溶液でのアパタイトの表面中和方法 |
| CN103814048A (zh) * | 2011-09-20 | 2014-05-21 | 生物辐射实验室股份有限公司 | 从生物分子制剂中去除杀病毒剂 |
| US9442050B2 (en) | 2012-02-14 | 2016-09-13 | Bio-Rad Laboratories, Inc. | Reducing pH excursions in ion exchange chromatography using displacing counter ions |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| JP6325446B2 (ja) * | 2012-09-18 | 2018-05-16 | 株式会社小糸製作所 | 吸着方法およびドラッグデリバリー用担持体 |
| US20150275195A1 (en) * | 2012-10-24 | 2015-10-01 | Genzyme Corporation | Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers |
| US10099157B2 (en) | 2014-06-23 | 2018-10-16 | Bio-Rad Laboratories, Inc. | Apatite in-situ restoration |
| CN105392569B (zh) | 2014-06-23 | 2019-08-20 | 生物辐射实验室股份有限公司 | 磷灰石预处理 |
| EP3368179A4 (en) * | 2015-10-28 | 2019-06-19 | Bio-rad Laboratories, Inc. | SURFACE NEUTRALIZATION OF APATIT |
| JP7137816B2 (ja) * | 2017-04-27 | 2022-09-15 | 国立研究開発法人産業技術総合研究所 | 塩基性の部位を備える物質を選択的に吸着する吸着材 |
| WO2019224660A1 (ja) * | 2018-05-24 | 2019-11-28 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 吸着剤の生産方法 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3737516A (en) * | 1971-03-05 | 1973-06-05 | Du Pont | Calcium-deficient hydroxylapatite for use in column chromatography |
| US4053561A (en) * | 1975-08-14 | 1977-10-11 | Stauffer Chemical Company | Removal of fluorine from phosphatic solutions |
| JP2507339B2 (ja) | 1986-08-11 | 1996-06-12 | 三井東圧化学株式会社 | 粗ｔＰＡの精製方法 |
| US4859342A (en) * | 1986-10-14 | 1989-08-22 | Suntory Limited | Process for industrially separating biopolymers |
| US5332503A (en) * | 1993-04-16 | 1994-07-26 | Baxter International Inc. | Process for purifying collagenase |
| US5744587A (en) * | 1995-06-07 | 1998-04-28 | Zymogenetics, Inc. | Method for purifying thrombopoietin |
| US5783217A (en) * | 1995-11-07 | 1998-07-21 | Etex Corporation | Low temperature calcium phosphate apatite and a method of its manufacture |
| ES2523599T3 (es) | 1997-12-03 | 2014-11-27 | Roche Diagnostics Gmbh | Eritropoyetina de gran actividad específica |
| JP4160652B2 (ja) | 1998-05-22 | 2008-10-01 | キリンファーマ株式会社 | エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子 |
| AU760633B2 (en) * | 1998-08-14 | 2003-05-22 | Merck Sharp & Dohme Corp. | Process for purifying human papillomavirus virus-like particles |
| US6156178A (en) | 1999-07-13 | 2000-12-05 | Molecular Dynamics, Inc. | Increased throughput analysis of small compounds using multiple temporally spaced injections |
| EP1415713A4 (en) | 2001-07-10 | 2006-02-01 | Yoshiyuki Nagae | COATING, POWDER AND METHOD FOR PRODUCING COATINGS |
| WO2003059935A2 (en) | 2001-12-21 | 2003-07-24 | Immunex Corporation | Methods for purifying protein |
| FR2834227A1 (fr) | 2001-12-27 | 2003-07-04 | Chiralsep Sarl | Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations |
| CN1311076C (zh) * | 2003-04-16 | 2007-04-18 | 普罗特奥姆技术公司 | 生产重组尿激酶的方法 |
| EP2336172B1 (en) * | 2003-10-27 | 2014-12-10 | Wyeth LLC | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
| ES2349112T3 (es) * | 2005-01-14 | 2010-12-28 | Bayer Healthcare Llc | Método de purificación de factor vii. |
| AU2006223180B2 (en) | 2005-03-11 | 2011-11-24 | Wyeth Llc | A method of weak partitioning chromatography |
| EP1866427A4 (en) * | 2005-03-30 | 2010-09-01 | Novo Nordisk As | METHOD OF MANUFACTURING PEPTIDES PRODUCED IN INSECT CELL LINES |
| US20100234577A1 (en) | 2006-06-14 | 2010-09-16 | Smithkline Beecham Corporation | Methods for purifying antibodies using ceramic hydroxyapatite |
| DK2061803T4 (da) | 2006-08-28 | 2022-12-05 | Ares Trading Sa | Fremgangsmåde til oprensning af fc-holdige proteiner |
| DK2134360T3 (en) | 2007-03-14 | 2016-02-22 | Takeda Vaccines Inc | Purification of virus-like particle |
| ES2496596T3 (es) | 2007-03-23 | 2014-09-19 | Asahi Kasei Pharma Corporation | Método para producir trombomodulina soluble de gran pureza |
| EP2166093B1 (en) | 2007-05-18 | 2015-09-16 | Tokyo Metropolitan Institute of Medical Science | Pharmaceutical composition for enzyme replacement therapy |
| CA2754884C (en) | 2007-06-21 | 2013-04-30 | Gen-Probe Incorporated | Methods of concentrating an analyte |
| EP2275431A3 (en) * | 2008-01-18 | 2011-05-25 | Bio-Rad Laboratories, Inc. | Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite chromotography |
| NZ586947A (en) | 2008-02-08 | 2012-11-30 | Ambrx Inc | Modified leptin polypeptides and their uses |
| US7635791B2 (en) * | 2008-04-17 | 2009-12-22 | Tpat Ip Llc | Biological buffers with wide buffering ranges |
| CN102119168A (zh) * | 2008-06-03 | 2011-07-06 | 帕特里有限公司 | 抗体纯化工艺 |
| CA2736141C (en) | 2008-09-23 | 2018-03-20 | F. Hoffmann-La Roche Ag | Purification of erythropoietin |
| EP2350130B1 (en) * | 2008-10-31 | 2018-10-03 | Wyeth LLC | Purification of acidic proteins using ceramic hydroxyapatite chromatography |
| HUE028341T2 (en) | 2009-06-16 | 2016-12-28 | Genzyme Corp | Improved methods for purification of recombinant aav vectors |
| WO2011088225A1 (en) * | 2010-01-15 | 2011-07-21 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| JP5881744B2 (ja) | 2011-02-02 | 2016-03-09 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | アルカリ溶液でのアパタイトの表面中和方法 |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
-
2011
- 2011-01-13 WO PCT/US2011/021158 patent/WO2011088225A1/en not_active Ceased
- 2011-01-13 JP JP2012549083A patent/JP5773454B2/ja active Active
- 2011-01-13 EP EP11733384.9A patent/EP2524215B1/en active Active
- 2011-01-13 CA CA2787009A patent/CA2787009C/en active Active
- 2011-01-13 US US13/006,022 patent/US8951807B2/en active Active
-
2015
- 2015-01-16 US US14/598,719 patent/US9212203B2/en active Active
- 2015-11-04 US US14/932,080 patent/US9914749B2/en active Active
-
2018
- 2018-01-24 US US15/879,239 patent/US10676502B2/en active Active
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