JP2013517284A5 - - Google Patents
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- Publication number
- JP2013517284A5 JP2013517284A5 JP2012549083A JP2012549083A JP2013517284A5 JP 2013517284 A5 JP2013517284 A5 JP 2013517284A5 JP 2012549083 A JP2012549083 A JP 2012549083A JP 2012549083 A JP2012549083 A JP 2012549083A JP 2013517284 A5 JP2013517284 A5 JP 2013517284A5
- Authority
- JP
- Japan
- Prior art keywords
- target molecule
- solution
- apatite
- solid surface
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 45
- 239000007787 solid Substances 0.000 claims description 22
- -1 amino compound Chemical class 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 18
- 229910052586 apatite Inorganic materials 0.000 claims description 15
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000000919 ceramic Substances 0.000 claims description 9
- 229910001413 alkali metal ion Inorganic materials 0.000 claims description 8
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 6
- 229910052587 fluorapatite Inorganic materials 0.000 claims description 5
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 5
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000004475 Arginine Substances 0.000 claims description 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 3
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004472 Lysine Substances 0.000 claims description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims description 2
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 claims description 2
- 239000007991 ACES buffer Substances 0.000 claims description 2
- 239000007995 HEPES buffer Substances 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 2
- 239000007987 MES buffer Substances 0.000 claims description 2
- 239000007993 MOPS buffer Substances 0.000 claims description 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims description 2
- 239000007990 PIPES buffer Substances 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 239000002158 endotoxin Substances 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229910052744 lithium Inorganic materials 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 150000003384 small molecules Chemical class 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29549910P | 2010-01-15 | 2010-01-15 | |
| US61/295,499 | 2010-01-15 | ||
| PCT/US2011/021158 WO2011088225A1 (en) | 2010-01-15 | 2011-01-13 | Surface neutralization of apatite |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013517284A JP2013517284A (ja) | 2013-05-16 |
| JP2013517284A5 true JP2013517284A5 (https=) | 2014-01-30 |
| JP5773454B2 JP5773454B2 (ja) | 2015-09-02 |
Family
ID=44278020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012549083A Active JP5773454B2 (ja) | 2010-01-15 | 2011-01-13 | アパタイトの表面中和方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (4) | US8951807B2 (https=) |
| EP (1) | EP2524215B1 (https=) |
| JP (1) | JP5773454B2 (https=) |
| CA (1) | CA2787009C (https=) |
| WO (1) | WO2011088225A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011088225A1 (en) * | 2010-01-15 | 2011-07-21 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| EP2670704B1 (en) | 2011-02-02 | 2018-12-12 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| US20130267690A1 (en) * | 2011-09-20 | 2013-10-10 | Bio-Rad Laboratories, Inc. | Removal of virucidal agents from biomolecule preparations |
| WO2013122834A1 (en) | 2012-02-14 | 2013-08-22 | Bio-Rad Laboratories, Inc. | REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| KR20150058388A (ko) * | 2012-09-18 | 2015-05-28 | 가부시키가이샤 고이토 세이사꾸쇼 | 흡착 방법, 흡착 분리 방법 및 드러그 딜리버리용 담지체 |
| WO2014066471A1 (en) | 2012-10-24 | 2014-05-01 | Genzyme Corporation | Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers |
| CN105407917B (zh) | 2014-06-23 | 2021-07-06 | 生物辐射实验室股份有限公司 | 磷灰石原位恢复 |
| US9802822B2 (en) | 2014-06-23 | 2017-10-31 | Bio-Rad Laboratories, Inc. | Apatite pretreatment |
| CN108348820A (zh) * | 2015-10-28 | 2018-07-31 | 生物辐射实验室股份有限公司 | 磷灰石的表面中和 |
| JP7137816B2 (ja) * | 2017-04-27 | 2022-09-15 | 国立研究開発法人産業技術総合研究所 | 塩基性の部位を備える物質を選択的に吸着する吸着材 |
| JP6951051B2 (ja) * | 2018-05-24 | 2021-10-20 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 吸着剤の生産方法 |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3737516A (en) * | 1971-03-05 | 1973-06-05 | Du Pont | Calcium-deficient hydroxylapatite for use in column chromatography |
| US4053561A (en) * | 1975-08-14 | 1977-10-11 | Stauffer Chemical Company | Removal of fluorine from phosphatic solutions |
| JP2507339B2 (ja) | 1986-08-11 | 1996-06-12 | 三井東圧化学株式会社 | 粗ｔＰＡの精製方法 |
| US4859342A (en) * | 1986-10-14 | 1989-08-22 | Suntory Limited | Process for industrially separating biopolymers |
| US5332503A (en) * | 1993-04-16 | 1994-07-26 | Baxter International Inc. | Process for purifying collagenase |
| US5744587A (en) * | 1995-06-07 | 1998-04-28 | Zymogenetics, Inc. | Method for purifying thrombopoietin |
| US5783217A (en) * | 1995-11-07 | 1998-07-21 | Etex Corporation | Low temperature calcium phosphate apatite and a method of its manufacture |
| WO1999028346A1 (de) | 1997-12-03 | 1999-06-10 | Roche Diagnostics Gmbh | Erythropoietin mit hoher spezifischer aktivität |
| JP4160652B2 (ja) | 1998-05-22 | 2008-10-01 | キリンファーマ株式会社 | エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子 |
| HUP0103091A3 (en) | 1998-08-14 | 2002-03-28 | Merck & Co Inc | Process for purifying human papillomavirus virus-like particles |
| US6156178A (en) | 1999-07-13 | 2000-12-05 | Molecular Dynamics, Inc. | Increased throughput analysis of small compounds using multiple temporally spaced injections |
| WO2003006159A1 (fr) | 2001-07-10 | 2003-01-23 | Yoshiyuki Nagae | Matiere de revetement, peinture et procede de production de la matiere de revetement |
| ES2322945T5 (es) * | 2001-12-21 | 2012-10-17 | Immunex Corporation | Métodos para purficación de proteína |
| FR2834227A1 (fr) | 2001-12-27 | 2003-07-04 | Chiralsep Sarl | Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations |
| CN1311076C (zh) * | 2003-04-16 | 2007-04-18 | 普罗特奥姆技术公司 | 生产重组尿激酶的方法 |
| KR101221862B1 (ko) * | 2003-10-27 | 2013-01-14 | 와이어쓰 엘엘씨 | 하이드록시아파타이트 크로마토그래피를 사용한 고분자량응집체의 제거 |
| PT1841863E (pt) * | 2005-01-14 | 2010-10-25 | Bayer Healthcare Llc | Método para a purificação de factor vii |
| EP1869065B1 (en) | 2005-03-11 | 2020-05-06 | Wyeth LLC | A method of weak partitioning chromatography |
| JP2008538181A (ja) * | 2005-03-30 | 2008-10-16 | ネオス テクノロジーズ インコーポレイテッド | 昆虫細胞系において増殖させたペプチドを生産するための製造方法 |
| JP2010510963A (ja) | 2006-06-14 | 2010-04-08 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー | セラミックヒドロキシアパタイトを使用する抗体の精製方法 |
| SI2061803T2 (sl) | 2006-08-28 | 2023-01-31 | Ares Trading S.A. | Postopek za čiščenje proteinov, ki vsebujejo FC |
| WO2008113011A2 (en) | 2007-03-14 | 2008-09-18 | Ligocyte Pharmaceuticals, Inc. | Virus like particle purification |
| CA2681294C (en) * | 2007-03-23 | 2014-05-06 | Asahi Kasei Pharma Corporation | Method for producing high-purity soluble thrombomodulin |
| WO2008143354A1 (ja) | 2007-05-18 | 2008-11-27 | Tokyo Metropolitan Organization For Medical Research | 酵素補充療法用医薬組成物 |
| US9458451B2 (en) | 2007-06-21 | 2016-10-04 | Gen-Probe Incorporated | Multi-channel optical measurement instrument |
| EP2238154B1 (en) * | 2008-01-18 | 2015-09-16 | Bio-Rad Laboratories, Inc. | Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography |
| MX344166B (es) | 2008-02-08 | 2016-12-07 | Ambrx Inc | Leptina-polipeptidos modificados y sus usos. |
| US7635791B2 (en) * | 2008-04-17 | 2009-12-22 | Tpat Ip Llc | Biological buffers with wide buffering ranges |
| CN102119168A (zh) * | 2008-06-03 | 2011-07-06 | 帕特里有限公司 | 抗体纯化工艺 |
| CN102164954B (zh) | 2008-09-23 | 2018-04-06 | 弗·哈夫曼-拉罗切有限公司 | 促红细胞生成素的纯化 |
| US8058407B2 (en) * | 2008-10-31 | 2011-11-15 | Wyeth Llc | Purification of acidic proteins using ceramic hydroxyapatite chromatography |
| KR20180000341A (ko) | 2009-06-16 | 2018-01-02 | 젠자임 코포레이션 | 재조합 ａａｖ 벡터에 대한 개선된 정제 방법 |
| WO2011088225A1 (en) * | 2010-01-15 | 2011-07-21 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| EP2670704B1 (en) | 2011-02-02 | 2018-12-12 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
-
2011
- 2011-01-13 WO PCT/US2011/021158 patent/WO2011088225A1/en not_active Ceased
- 2011-01-13 US US13/006,022 patent/US8951807B2/en active Active
- 2011-01-13 JP JP2012549083A patent/JP5773454B2/ja active Active
- 2011-01-13 CA CA2787009A patent/CA2787009C/en active Active
- 2011-01-13 EP EP11733384.9A patent/EP2524215B1/en active Active
-
2015
- 2015-01-16 US US14/598,719 patent/US9212203B2/en active Active
- 2015-11-04 US US14/932,080 patent/US9914749B2/en active Active
-
2018
- 2018-01-24 US US15/879,239 patent/US10676502B2/en active Active
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