JP5773454B2 - アパタイトの表面中和方法 - Google Patents

アパタイトの表面中和方法 Download PDF

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Publication number
JP5773454B2
JP5773454B2 JP2012549083A JP2012549083A JP5773454B2 JP 5773454 B2 JP5773454 B2 JP 5773454B2 JP 2012549083 A JP2012549083 A JP 2012549083A JP 2012549083 A JP2012549083 A JP 2012549083A JP 5773454 B2 JP5773454 B2 JP 5773454B2
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Prior art keywords
apatite
target molecule
solution
concentration
column
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Japanese (ja)
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JP2013517284A5 (https=
JP2013517284A (ja
Inventor
ラリー ジェイ． カミングス
ラリー ジェイ． カミングス
マーク エイ． スナイダー
マーク エイ． スナイダー
Original Assignee
バイオ−ラッド ラボラトリーズ インコーポレーティッド
バイオ−ラッド ラボラトリーズ インコーポレーティッド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2012549083A 2010-01-15 2011-01-13 アパタイトの表面中和方法 Active JP5773454B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29549910P 2010-01-15 2010-01-15
US61/295,499 2010-01-15
PCT/US2011/021158 WO2011088225A1 (en) 2010-01-15 2011-01-13 Surface neutralization of apatite

Publications (3)

Publication Number Publication Date
JP2013517284A JP2013517284A (ja) 2013-05-16
JP2013517284A5 JP2013517284A5 (https=) 2014-01-30
JP5773454B2 true JP5773454B2 (ja) 2015-09-02

Family

ID=44278020

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012549083A Active JP5773454B2 (ja) 2010-01-15 2011-01-13 アパタイトの表面中和方法

Country Status (5)

Country Link
US (4) US8951807B2 (https=)
EP (1) EP2524215B1 (https=)
JP (1) JP5773454B2 (https=)
CA (1) CA2787009C (https=)
WO (1) WO2011088225A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011088225A1 (en) * 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
JP5881744B2 (ja) * 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
CN103814048A (zh) * 2011-09-20 2014-05-21 生物辐射实验室股份有限公司 从生物分子制剂中去除杀病毒剂
US9442050B2 (en) 2012-02-14 2016-09-13 Bio-Rad Laboratories, Inc. Reducing pH excursions in ion exchange chromatography using displacing counter ions
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
JP6325446B2 (ja) * 2012-09-18 2018-05-16 株式会社小糸製作所 吸着方法およびドラッグデリバリー用担持体
US20150275195A1 (en) * 2012-10-24 2015-10-01 Genzyme Corporation Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
US10099157B2 (en) 2014-06-23 2018-10-16 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
CN105392569B (zh) 2014-06-23 2019-08-20 生物辐射实验室股份有限公司 磷灰石预处理
EP3368179A4 (en) * 2015-10-28 2019-06-19 Bio-rad Laboratories, Inc. SURFACE NEUTRALIZATION OF APATIT
JP7137816B2 (ja) * 2017-04-27 2022-09-15 国立研究開発法人産業技術総合研究所 塩基性の部位を備える物質を選択的に吸着する吸着材
WO2019224660A1 (ja) * 2018-05-24 2019-11-28 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3737516A (en) * 1971-03-05 1973-06-05 Du Pont Calcium-deficient hydroxylapatite for use in column chromatography
US4053561A (en) * 1975-08-14 1977-10-11 Stauffer Chemical Company Removal of fluorine from phosphatic solutions
JP2507339B2 (ja) 1986-08-11 1996-06-12 三井東圧化学株式会社 粗ｔＰＡの精製方法
US4859342A (en) * 1986-10-14 1989-08-22 Suntory Limited Process for industrially separating biopolymers
US5332503A (en) * 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase
US5744587A (en) * 1995-06-07 1998-04-28 Zymogenetics, Inc. Method for purifying thrombopoietin
US5783217A (en) * 1995-11-07 1998-07-21 Etex Corporation Low temperature calcium phosphate apatite and a method of its manufacture
ES2523599T3 (es) 1997-12-03 2014-11-27 Roche Diagnostics Gmbh Eritropoyetina de gran actividad específica
JP4160652B2 (ja) 1998-05-22 2008-10-01 キリンファーマ株式会社 エンド−β−Ｎ−アセチルグルコサミニダーゼ遺伝子
AU760633B2 (en) * 1998-08-14 2003-05-22 Merck Sharp & Dohme Corp. Process for purifying human papillomavirus virus-like particles
US6156178A (en) 1999-07-13 2000-12-05 Molecular Dynamics, Inc. Increased throughput analysis of small compounds using multiple temporally spaced injections
EP1415713A4 (en) 2001-07-10 2006-02-01 Yoshiyuki Nagae COATING, POWDER AND METHOD FOR PRODUCING COATINGS
WO2003059935A2 (en) 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
FR2834227A1 (fr) 2001-12-27 2003-07-04 Chiralsep Sarl Materiaux supports optiquement actifs, leur procede de preparation et leurs utilisations
CN1311076C (zh) * 2003-04-16 2007-04-18 普罗特奥姆技术公司 生产重组尿激酶的方法
EP2336172B1 (en) * 2003-10-27 2014-12-10 Wyeth LLC Removal of high molecular weight aggregates using hydroxyapatite chromatography
ES2349112T3 (es) * 2005-01-14 2010-12-28 Bayer Healthcare Llc Método de purificación de factor vii.
AU2006223180B2 (en) 2005-03-11 2011-11-24 Wyeth Llc A method of weak partitioning chromatography
EP1866427A4 (en) * 2005-03-30 2010-09-01 Novo Nordisk As METHOD OF MANUFACTURING PEPTIDES PRODUCED IN INSECT CELL LINES
US20100234577A1 (en) 2006-06-14 2010-09-16 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
DK2061803T4 (da) 2006-08-28 2022-12-05 Ares Trading Sa Fremgangsmåde til oprensning af fc-holdige proteiner
DK2134360T3 (en) 2007-03-14 2016-02-22 Takeda Vaccines Inc Purification of virus-like particle
ES2496596T3 (es) 2007-03-23 2014-09-19 Asahi Kasei Pharma Corporation Método para producir trombomodulina soluble de gran pureza
EP2166093B1 (en) 2007-05-18 2015-09-16 Tokyo Metropolitan Institute of Medical Science Pharmaceutical composition for enzyme replacement therapy
CA2754884C (en) 2007-06-21 2013-04-30 Gen-Probe Incorporated Methods of concentrating an analyte
EP2275431A3 (en) * 2008-01-18 2011-05-25 Bio-Rad Laboratories, Inc. Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite chromotography
NZ586947A (en) 2008-02-08 2012-11-30 Ambrx Inc Modified leptin polypeptides and their uses
US7635791B2 (en) * 2008-04-17 2009-12-22 Tpat Ip Llc Biological buffers with wide buffering ranges
CN102119168A (zh) * 2008-06-03 2011-07-06 帕特里有限公司 抗体纯化工艺
CA2736141C (en) 2008-09-23 2018-03-20 F. Hoffmann-La Roche Ag Purification of erythropoietin
EP2350130B1 (en) * 2008-10-31 2018-10-03 Wyeth LLC Purification of acidic proteins using ceramic hydroxyapatite chromatography
HUE028341T2 (en) 2009-06-16 2016-12-28 Genzyme Corp Improved methods for purification of recombinant aav vectors
WO2011088225A1 (en) * 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
JP5881744B2 (ja) 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins

Also Published As

Publication number Publication date
US9212203B2 (en) 2015-12-15
CA2787009A1 (en) 2011-07-21
US10676502B2 (en) 2020-06-09
EP2524215A1 (en) 2012-11-21
US20150191505A1 (en) 2015-07-09
EP2524215B1 (en) 2018-12-26
WO2011088225A1 (en) 2011-07-21
CA2787009C (en) 2018-04-03
US9914749B2 (en) 2018-03-13
US8951807B2 (en) 2015-02-10
US20110178276A1 (en) 2011-07-21
US20160052961A1 (en) 2016-02-25
US20180155390A1 (en) 2018-06-07
JP2013517284A (ja) 2013-05-16
EP2524215A4 (en) 2014-08-20

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