JP2012510500A5 - - Google Patents
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- Publication number
- JP2012510500A5 JP2012510500A5 JP2011538995A JP2011538995A JP2012510500A5 JP 2012510500 A5 JP2012510500 A5 JP 2012510500A5 JP 2011538995 A JP2011538995 A JP 2011538995A JP 2011538995 A JP2011538995 A JP 2011538995A JP 2012510500 A5 JP2012510500 A5 JP 2012510500A5
- Authority
- JP
- Japan
- Prior art keywords
- factor
- gla
- polypeptide
- elution
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 17
- 229920001184 polypeptide Polymers 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims 10
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims 8
- 102100022641 Coagulation factor IX Human genes 0.000 claims 6
- 102100023804 Coagulation factor VII Human genes 0.000 claims 6
- 108010076282 Factor IX Proteins 0.000 claims 6
- 108010023321 Factor VII Proteins 0.000 claims 6
- 108010054265 Factor VIIa Proteins 0.000 claims 6
- 238000010828 elution Methods 0.000 claims 6
- 229960004222 factor ix Drugs 0.000 claims 6
- 229940012413 factor vii Drugs 0.000 claims 6
- 229940012414 factor viia Drugs 0.000 claims 6
- UHBYWPGGCSDKFX-VKHMYHEASA-N gamma-carboxy-L-glutamic acid Chemical compound OC(=O)[C@@H](N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-VKHMYHEASA-N 0.000 claims 5
- 239000000463 material Substances 0.000 claims 5
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims 4
- 239000005695 Ammonium acetate Substances 0.000 claims 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 4
- 229940043376 ammonium acetate Drugs 0.000 claims 4
- 235000019257 ammonium acetate Nutrition 0.000 claims 4
- 235000019270 ammonium chloride Nutrition 0.000 claims 4
- 238000005349 anion exchange Methods 0.000 claims 4
- 239000001632 sodium acetate Substances 0.000 claims 4
- 235000017281 sodium acetate Nutrition 0.000 claims 4
- 239000012149 elution buffer Substances 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 2
- 150000003839 salts Chemical group 0.000 claims 2
- 108010014173 Factor X Proteins 0.000 claims 1
- 102100031487 Growth arrest-specific protein 6 Human genes 0.000 claims 1
- 101000923005 Homo sapiens Growth arrest-specific protein 6 Proteins 0.000 claims 1
- 101100216047 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) gla-1 gene Proteins 0.000 claims 1
- 108090000573 Osteocalcin Proteins 0.000 claims 1
- 102000004067 Osteocalcin Human genes 0.000 claims 1
- 101800004937 Protein C Proteins 0.000 claims 1
- 102000017975 Protein C Human genes 0.000 claims 1
- 108010066124 Protein S Proteins 0.000 claims 1
- 229940096437 Protein S Drugs 0.000 claims 1
- 108010094028 Prothrombin Proteins 0.000 claims 1
- 102100027378 Prothrombin Human genes 0.000 claims 1
- 101800001700 Saposin-D Proteins 0.000 claims 1
- 102100028885 Vitamin K-dependent protein S Human genes 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000006167 equilibration buffer Substances 0.000 claims 1
- 229940012426 factor x Drugs 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 108010057546 matrix Gla protein Proteins 0.000 claims 1
- 102000043253 matrix Gla protein Human genes 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108010025221 plasma protein Z Proteins 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 229960000856 protein c Drugs 0.000 claims 1
- 229940039716 prothrombin Drugs 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08170454 | 2008-12-02 | ||
| EP08170454.6 | 2008-12-02 | ||
| PCT/EP2009/066149 WO2010063717A1 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012510500A JP2012510500A (ja) | 2012-05-10 |
| JP2012510500A5 true JP2012510500A5 (enExample) | 2013-01-10 |
| JP5788803B2 JP5788803B2 (ja) | 2015-10-07 |
Family
ID=40404199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011538995A Active JP5788803B2 (ja) | 2008-12-02 | 2009-12-01 | ポリペプチド精製 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20110287518A1 (enExample) |
| EP (1) | EP2373677B1 (enExample) |
| JP (1) | JP5788803B2 (enExample) |
| KR (1) | KR101821143B1 (enExample) |
| CN (1) | CN102239175A (enExample) |
| AU (1) | AU2009324204B2 (enExample) |
| BR (1) | BRPI0922716A2 (enExample) |
| CA (1) | CA2742246A1 (enExample) |
| ES (1) | ES2576109T3 (enExample) |
| MX (1) | MX2011005474A (enExample) |
| RU (1) | RU2544860C2 (enExample) |
| WO (1) | WO2010063717A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201407983VA (en) * | 2009-12-18 | 2015-01-29 | Csl Ltd | Method of purifying polypeptides |
| EP2526115B1 (en) | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
| CN107915769A (zh) * | 2011-03-29 | 2018-04-17 | 葛兰素史密斯克莱有限责任公司 | 用于蛋白纯化的缓冲液体系 |
| CA2838814A1 (en) | 2011-08-25 | 2013-02-28 | F.Hoffmann-La Roche Ag | Cation and anion exchange chromatography method |
| EP3262409B1 (en) * | 2015-02-25 | 2020-03-25 | Omrix Biopharmaceuticals Ltd. | Method for purifying and quantifying thrombin and its degradation polypeptides |
| WO2016207328A1 (en) * | 2015-06-24 | 2016-12-29 | Glycotope Gmbh | PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES |
| CA3027143A1 (en) * | 2016-07-15 | 2018-01-18 | F. Hoffmann-La Roche Ag | Method for purifying pegylated erythropoietin |
| KR20220079844A (ko) * | 2019-10-14 | 2022-06-14 | 피어스 바이오테크놀로지, 인크 | 펩티드 정제 제제 및 방법 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981952A (en) | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| IT1262899B (it) * | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| KR101204712B1 (ko) * | 2003-03-18 | 2012-11-27 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | Ｇｌａ-잔기 함유 세린 프로테아제의 제조 방법 |
| JP2008525381A (ja) * | 2004-12-23 | 2008-07-17 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少 |
| EP2125866B1 (en) * | 2007-02-28 | 2013-05-22 | Baxter International Inc. | Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules |
-
2009
- 2009-12-01 AU AU2009324204A patent/AU2009324204B2/en not_active Ceased
- 2009-12-01 ES ES09793497.0T patent/ES2576109T3/es active Active
- 2009-12-01 JP JP2011538995A patent/JP5788803B2/ja active Active
- 2009-12-01 KR KR1020117014183A patent/KR101821143B1/ko not_active Expired - Fee Related
- 2009-12-01 EP EP09793497.0A patent/EP2373677B1/en active Active
- 2009-12-01 BR BRPI0922716A patent/BRPI0922716A2/pt not_active Application Discontinuation
- 2009-12-01 US US13/131,757 patent/US20110287518A1/en not_active Abandoned
- 2009-12-01 RU RU2011125656/04A patent/RU2544860C2/ru active
- 2009-12-01 WO PCT/EP2009/066149 patent/WO2010063717A1/en not_active Ceased
- 2009-12-01 CN CN2009801490787A patent/CN102239175A/zh active Pending
- 2009-12-01 CA CA2742246A patent/CA2742246A1/en not_active Abandoned
- 2009-12-01 MX MX2011005474A patent/MX2011005474A/es active IP Right Grant
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