CN102239175A - 多肽的纯化 - Google Patents

多肽的纯化 Download PDF

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Publication number
CN102239175A
CN102239175A CN2009801490787A CN200980149078A CN102239175A CN 102239175 A CN102239175 A CN 102239175A CN 2009801490787 A CN2009801490787 A CN 2009801490787A CN 200980149078 A CN200980149078 A CN 200980149078A CN 102239175 A CN102239175 A CN 102239175A
Authority
CN
China
Prior art keywords
polypeptide
gla
sample
buffer
anion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2009801490787A
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English (en)
Chinese (zh)
Inventor
J·R·布杰克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk Health Care AG
Original Assignee
Novo Nordisk Health Care AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Health Care AG filed Critical Novo Nordisk Health Care AG
Publication of CN102239175A publication Critical patent/CN102239175A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
CN2009801490787A 2008-12-02 2009-12-01 多肽的纯化 Pending CN102239175A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08170454.6 2008-12-02
EP08170454 2008-12-02
PCT/EP2009/066149 WO2010063717A1 (en) 2008-12-02 2009-12-01 Polypeptide purification

Publications (1)

Publication Number Publication Date
CN102239175A true CN102239175A (zh) 2011-11-09

Family

ID=40404199

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801490787A Pending CN102239175A (zh) 2008-12-02 2009-12-01 多肽的纯化

Country Status (12)

Country Link
US (1) US20110287518A1 (enExample)
EP (1) EP2373677B1 (enExample)
JP (1) JP5788803B2 (enExample)
KR (1) KR101821143B1 (enExample)
CN (1) CN102239175A (enExample)
AU (1) AU2009324204B2 (enExample)
BR (1) BRPI0922716A2 (enExample)
CA (1) CA2742246A1 (enExample)
ES (1) ES2576109T3 (enExample)
MX (1) MX2011005474A (enExample)
RU (1) RU2544860C2 (enExample)
WO (1) WO2010063717A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107406840A (zh) * 2015-02-25 2017-11-28 奥姆里克斯生物药品有限公司 用于纯化和定量凝血酶及其降解多肽的方法
CN114599971A (zh) * 2019-10-14 2022-06-07 皮尔斯生物科技有限公司 肽纯化配制物和方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3715356B1 (en) 2009-12-18 2023-10-11 CSL Limited Method of purifying polypeptides
US9896677B2 (en) 2010-01-18 2018-02-20 Novo Nordisk Health Care Ag Purification of blood coagulation factors
WO2012135415A1 (en) 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification
WO2013026803A1 (en) 2011-08-25 2013-02-28 F. Hoffmann-La Roche Ag Cation and anion exchange chromatography method
WO2016207328A1 (en) * 2015-06-24 2016-12-29 Glycotope Gmbh PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES
KR102820834B1 (ko) * 2016-07-15 2025-06-13 에프. 호프만-라 로슈 아게 Ｐｅｇ화 에리트로포이에틴을 정제하기 위한 방법
JP2023090132A (ja) * 2021-12-17 2023-06-29 Ｋｍバイオロジクス株式会社 ビタミンＫ依存性タンパク質のγ－カルボキシグルタミン酸修飾度の分析方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363126A2 (en) * 1988-10-04 1990-04-11 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
EP0617049A1 (en) * 1992-03-22 1994-09-28 SCLAVO S.p.A. Process for the isolation of highly purified factors IX, X and II from prothrombin complex or human plasma
CN101098883A (zh) * 2004-12-23 2008-01-02 诺和诺德医疗保健公司 减少含有目的维生素k依赖性蛋白质的组合物中的蛋白质污染物的含量

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3103869A1 (en) * 2003-03-18 2016-12-14 Novo Nordisk Health Care AG Method for the production of factor vii polypeptides
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0363126A2 (en) * 1988-10-04 1990-04-11 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
EP0617049A1 (en) * 1992-03-22 1994-09-28 SCLAVO S.p.A. Process for the isolation of highly purified factors IX, X and II from prothrombin complex or human plasma
CN101098883A (zh) * 2004-12-23 2008-01-02 诺和诺德医疗保健公司 减少含有目的维生素k依赖性蛋白质的组合物中的蛋白质污染物的含量

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GILLIS S ET AL: "Gamma-Carboxyglutamic Acids 36 and 40 Do Not Contribute to Human Factor IX Function", 《PROTEIN SCIENCE》 *
MALHOTRA ET AL: "Partially carboxylated prothrombins III. Carboxylation studies", 《BIOCHIMICA ET BIOPHYSICA ACTA》 *
MYLES LINDSAY ET AL.: "Purification of recombinant DNA-derived factor IX produced in transgenic pig milk and fractionation of active and inactive subpopulations", 《JOURNAL OF CHROMATOGRAPHY A》 *
YAN S C B ET.AL: "Characterization and novel purification of recombinant human protein C from three mammalian cell lines", 《BIO/TECHNOLOGY》 *
王建华，等: "人高纯度凝血因子IX的研制", 《中华血液学杂志》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107406840A (zh) * 2015-02-25 2017-11-28 奥姆里克斯生物药品有限公司 用于纯化和定量凝血酶及其降解多肽的方法
US11008560B2 (en) 2015-02-25 2021-05-18 Omrix Biopharmaceuticals Ltd. Method for purifying and quantifying thrombin and its degradation polypeptides
CN107406840B (zh) * 2015-02-25 2021-05-28 奥姆里克斯生物药品有限公司 用于纯化和定量凝血酶及其降解多肽的方法
US11149263B2 (en) 2015-02-25 2021-10-19 Omrix Biopharmaceuticals Ltd. Method for purifying and quantifying thrombin and its degradation polypeptides
US12351843B2 (en) 2015-02-25 2025-07-08 Omrix Biopharmaceuticals Ltd. Method for purifying and quantifying thrombin and its degradation polypeptides
CN114599971A (zh) * 2019-10-14 2022-06-07 皮尔斯生物科技有限公司 肽纯化配制物和方法

Also Published As

Publication number Publication date
MX2011005474A (es) 2011-06-16
KR101821143B1 (ko) 2018-01-23
AU2009324204B2 (en) 2014-10-16
AU2009324204A1 (en) 2010-06-10
WO2010063717A1 (en) 2010-06-10
BRPI0922716A2 (pt) 2016-11-01
RU2011125656A (ru) 2013-01-10
JP5788803B2 (ja) 2015-10-07
ES2576109T3 (es) 2016-07-05
US20110287518A1 (en) 2011-11-24
RU2544860C2 (ru) 2015-03-20
JP2012510500A (ja) 2012-05-10
EP2373677B1 (en) 2016-03-23
KR20110099251A (ko) 2011-09-07
EP2373677A1 (en) 2011-10-12
CA2742246A1 (en) 2010-06-10

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PB01 Publication
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SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20111109

RJ01 Rejection of invention patent application after publication