CA2742246A1 - Polypeptide purification - Google Patents
Polypeptide purification Download PDFInfo
- Publication number
- CA2742246A1 CA2742246A1 CA2742246A CA2742246A CA2742246A1 CA 2742246 A1 CA2742246 A1 CA 2742246A1 CA 2742246 A CA2742246 A CA 2742246A CA 2742246 A CA2742246 A CA 2742246A CA 2742246 A1 CA2742246 A1 CA 2742246A1
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- CA
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- Prior art keywords
- polypeptide
- gla
- factor
- sample
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 229920000136 polysorbate Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000003805 procoagulant Substances 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 108020001568 subdomains Proteins 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6437—Coagulation factor VIIa (3.4.21.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08170454 | 2008-12-02 | ||
| EP08170454.6 | 2008-12-02 | ||
| PCT/EP2009/066149 WO2010063717A1 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2742246A1 true CA2742246A1 (en) | 2010-06-10 |
Family
ID=40404199
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2742246A Abandoned CA2742246A1 (en) | 2008-12-02 | 2009-12-01 | Polypeptide purification |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20110287518A1 (enExample) |
| EP (1) | EP2373677B1 (enExample) |
| JP (1) | JP5788803B2 (enExample) |
| KR (1) | KR101821143B1 (enExample) |
| CN (1) | CN102239175A (enExample) |
| AU (1) | AU2009324204B2 (enExample) |
| BR (1) | BRPI0922716A2 (enExample) |
| CA (1) | CA2742246A1 (enExample) |
| ES (1) | ES2576109T3 (enExample) |
| MX (1) | MX2011005474A (enExample) |
| RU (1) | RU2544860C2 (enExample) |
| WO (1) | WO2010063717A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201407983VA (en) * | 2009-12-18 | 2015-01-29 | Csl Ltd | Method of purifying polypeptides |
| EP2526115B1 (en) | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
| CN107915769A (zh) * | 2011-03-29 | 2018-04-17 | 葛兰素史密斯克莱有限责任公司 | 用于蛋白纯化的缓冲液体系 |
| CA2838814A1 (en) | 2011-08-25 | 2013-02-28 | F.Hoffmann-La Roche Ag | Cation and anion exchange chromatography method |
| EP3262409B1 (en) * | 2015-02-25 | 2020-03-25 | Omrix Biopharmaceuticals Ltd. | Method for purifying and quantifying thrombin and its degradation polypeptides |
| WO2016207328A1 (en) * | 2015-06-24 | 2016-12-29 | Glycotope Gmbh | PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES |
| CA3027143A1 (en) * | 2016-07-15 | 2018-01-18 | F. Hoffmann-La Roche Ag | Method for purifying pegylated erythropoietin |
| KR20220079844A (ko) * | 2019-10-14 | 2022-06-14 | 피어스 바이오테크놀로지, 인크 | 펩티드 정제 제제 및 방법 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981952A (en) | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| IT1262899B (it) * | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| KR101204712B1 (ko) * | 2003-03-18 | 2012-11-27 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | Ｇｌａ-잔기 함유 세린 프로테아제의 제조 방법 |
| JP2008525381A (ja) * | 2004-12-23 | 2008-07-17 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少 |
| EP2125866B1 (en) * | 2007-02-28 | 2013-05-22 | Baxter International Inc. | Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules |
-
2009
- 2009-12-01 AU AU2009324204A patent/AU2009324204B2/en not_active Ceased
- 2009-12-01 ES ES09793497.0T patent/ES2576109T3/es active Active
- 2009-12-01 JP JP2011538995A patent/JP5788803B2/ja active Active
- 2009-12-01 KR KR1020117014183A patent/KR101821143B1/ko not_active Expired - Fee Related
- 2009-12-01 EP EP09793497.0A patent/EP2373677B1/en active Active
- 2009-12-01 BR BRPI0922716A patent/BRPI0922716A2/pt not_active Application Discontinuation
- 2009-12-01 US US13/131,757 patent/US20110287518A1/en not_active Abandoned
- 2009-12-01 RU RU2011125656/04A patent/RU2544860C2/ru active
- 2009-12-01 WO PCT/EP2009/066149 patent/WO2010063717A1/en not_active Ceased
- 2009-12-01 CN CN2009801490787A patent/CN102239175A/zh active Pending
- 2009-12-01 CA CA2742246A patent/CA2742246A1/en not_active Abandoned
- 2009-12-01 MX MX2011005474A patent/MX2011005474A/es active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| KR101821143B1 (ko) | 2018-01-23 |
| AU2009324204B2 (en) | 2014-10-16 |
| BRPI0922716A2 (pt) | 2016-11-01 |
| MX2011005474A (es) | 2011-06-16 |
| RU2011125656A (ru) | 2013-01-10 |
| JP2012510500A (ja) | 2012-05-10 |
| US20110287518A1 (en) | 2011-11-24 |
| EP2373677A1 (en) | 2011-10-12 |
| RU2544860C2 (ru) | 2015-03-20 |
| AU2009324204A1 (en) | 2010-06-10 |
| ES2576109T3 (es) | 2016-07-05 |
| JP5788803B2 (ja) | 2015-10-07 |
| EP2373677B1 (en) | 2016-03-23 |
| WO2010063717A1 (en) | 2010-06-10 |
| CN102239175A (zh) | 2011-11-09 |
| KR20110099251A (ko) | 2011-09-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |
Effective date: 20141202 |