RU2544860C2 - Очистка полипептидов - Google Patents

Очистка полипептидов Download PDF

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Publication number
RU2544860C2
RU2544860C2 RU2011125656/04A RU2011125656A RU2544860C2 RU 2544860 C2 RU2544860 C2 RU 2544860C2 RU 2011125656/04 A RU2011125656/04 A RU 2011125656/04A RU 2011125656 A RU2011125656 A RU 2011125656A RU 2544860 C2 RU2544860 C2 RU 2544860C2
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RU
Russia
Prior art keywords
polypeptide
gla
buffer
gamma
sample
Prior art date
Application number
RU2011125656/04A
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English (en)
Russian (ru)
Other versions
RU2011125656A (ru
Inventor
Яис Розе БЬЕЛКЕ
Original Assignee
Ново Нордиск Хелс Кеа Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Ново Нордиск Хелс Кеа Аг filed Critical Ново Нордиск Хелс Кеа Аг
Publication of RU2011125656A publication Critical patent/RU2011125656A/ru
Application granted granted Critical
Publication of RU2544860C2 publication Critical patent/RU2544860C2/ru

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
RU2011125656/04A 2008-12-02 2009-12-01 Очистка полипептидов RU2544860C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08170454 2008-12-02
EP08170454.6 2008-12-02
PCT/EP2009/066149 WO2010063717A1 (en) 2008-12-02 2009-12-01 Polypeptide purification

Publications (2)

Publication Number Publication Date
RU2011125656A RU2011125656A (ru) 2013-01-10
RU2544860C2 true RU2544860C2 (ru) 2015-03-20

Family

ID=40404199

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2011125656/04A RU2544860C2 (ru) 2008-12-02 2009-12-01 Очистка полипептидов

Country Status (12)

Country Link
US (1) US20110287518A1 (enExample)
EP (1) EP2373677B1 (enExample)
JP (1) JP5788803B2 (enExample)
KR (1) KR101821143B1 (enExample)
CN (1) CN102239175A (enExample)
AU (1) AU2009324204B2 (enExample)
BR (1) BRPI0922716A2 (enExample)
CA (1) CA2742246A1 (enExample)
ES (1) ES2576109T3 (enExample)
MX (1) MX2011005474A (enExample)
RU (1) RU2544860C2 (enExample)
WO (1) WO2010063717A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201407983VA (en) * 2009-12-18 2015-01-29 Csl Ltd Method of purifying polypeptides
EP2526115B1 (en) 2010-01-18 2018-08-01 Novo Nordisk Health Care AG Purification of blood coagulation factors
CN107915769A (zh) * 2011-03-29 2018-04-17 葛兰素史密斯克莱有限责任公司 用于蛋白纯化的缓冲液体系
CA2838814A1 (en) 2011-08-25 2013-02-28 F.Hoffmann-La Roche Ag Cation and anion exchange chromatography method
EP3262409B1 (en) * 2015-02-25 2020-03-25 Omrix Biopharmaceuticals Ltd. Method for purifying and quantifying thrombin and its degradation polypeptides
WO2016207328A1 (en) * 2015-06-24 2016-12-29 Glycotope Gmbh PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES
CA3027143A1 (en) * 2016-07-15 2018-01-18 F. Hoffmann-La Roche Ag Method for purifying pegylated erythropoietin
KR20220079844A (ko) * 2019-10-14 2022-06-14 피어스 바이오테크놀로지, 인크 펩티드 정제 제제 및 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2364626C2 (ru) * 2003-03-18 2009-08-20 Ново Нордиск Хелт Кэр Аг Способ получения, способ очистки и способ стабилизации полипептидов фактора vii, ix и x

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
JP2008525381A (ja) * 2004-12-23 2008-07-17 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少
EP2125866B1 (en) * 2007-02-28 2013-05-22 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2364626C2 (ru) * 2003-03-18 2009-08-20 Ново Нордиск Хелт Кэр Аг Способ получения, способ очистки и способ стабилизации полипептидов фактора vii, ix и x

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GILLIS S ET AL: "GAMMA-CARBOXYGLUTAMIC ACIDS 36 AND 40 DO NOT CONTRIBUTE TO HUMAN FACTOR IX FUNCTION" PROTEIN SCIENCE, 1997. vol. 6, no. 1, pages 185-196. MALHOTRA ET AL: "Partially carboxylated prothrombins - III. Carboxylation studies" BIOCHIMICA ET BIOPHYSICA ACTA - PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGIE, ELSEVIER SCIENCE BV, 1983, vol. 746, no. 1-2, pages 81-86. YAN S C B ET AL: "CHARACTERIZATION AND NOVEL PURIFICATION OF RECOMBINANT HUMAN PROTEIN C FROM THREE MAMMALIAN CELL LINES" BIO/TECHNOLOGY, 1990,vol. 8, no. 7, pages 655-661. BURNIER J P ET AL: "GAMMA CARBOXY GLUTAMIC-ACID" MOLECULAR AND CELLULAR BIOCHEMISTRY, 1981, vol. 39, no. PART 2, pages 191-208. MALHOTRA O P: "DICOUMAROL-INDUCED 9-GAMMA CARBOXYGLUTAMIC ACID PROTHROMBIN ISOLATION AND COMPARISON WITH 6-GAMMA CARBOXYGLUTAMIC ACID 7-GAMMA CARBOXYGLUTAMIC ACID 8-GAMMA CARBOXYGLUTAMIC ACID AND 10-GAMMA CARBOXYGLUTAMIC ACID ISOMERS" BIOCHEMISTRY AND CELL BIOLOGY, 1990, vol. 68, no. 4, pages 705-715. OSTERUD B ET AL: "Huma *

Also Published As

Publication number Publication date
KR101821143B1 (ko) 2018-01-23
AU2009324204B2 (en) 2014-10-16
BRPI0922716A2 (pt) 2016-11-01
MX2011005474A (es) 2011-06-16
RU2011125656A (ru) 2013-01-10
JP2012510500A (ja) 2012-05-10
US20110287518A1 (en) 2011-11-24
EP2373677A1 (en) 2011-10-12
CA2742246A1 (en) 2010-06-10
AU2009324204A1 (en) 2010-06-10
ES2576109T3 (es) 2016-07-05
JP5788803B2 (ja) 2015-10-07
EP2373677B1 (en) 2016-03-23
WO2010063717A1 (en) 2010-06-10
CN102239175A (zh) 2011-11-09
KR20110099251A (ko) 2011-09-07

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