JP5788803B2 - ポリペプチド精製 - Google Patents

ポリペプチド精製 Download PDF

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Publication number
JP5788803B2
JP5788803B2 JP2011538995A JP2011538995A JP5788803B2 JP 5788803 B2 JP5788803 B2 JP 5788803B2 JP 2011538995 A JP2011538995 A JP 2011538995A JP 2011538995 A JP2011538995 A JP 2011538995A JP 5788803 B2 JP5788803 B2 JP 5788803B2
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JP
Japan
Prior art keywords
polypeptide
gla
anion exchange
sample
buffer
Prior art date
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JP2011538995A
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English (en)
Japanese (ja)
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JP2012510500A (ja
JP2012510500A5 (enExample
Inventor
ヤイス・ローズ・ビエルケ
Original Assignee
ノヴォ・ノルディスク・ヘルス・ケア・アーゲー
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Publication of JP2012510500A publication Critical patent/JP2012510500A/ja
Publication of JP2012510500A5 publication Critical patent/JP2012510500A5/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
JP2011538995A 2008-12-02 2009-12-01 ポリペプチド精製 Active JP5788803B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08170454 2008-12-02
EP08170454.6 2008-12-02
PCT/EP2009/066149 WO2010063717A1 (en) 2008-12-02 2009-12-01 Polypeptide purification

Publications (3)

Publication Number Publication Date
JP2012510500A JP2012510500A (ja) 2012-05-10
JP2012510500A5 JP2012510500A5 (enExample) 2013-01-10
JP5788803B2 true JP5788803B2 (ja) 2015-10-07

Family

ID=40404199

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011538995A Active JP5788803B2 (ja) 2008-12-02 2009-12-01 ポリペプチド精製

Country Status (12)

Country Link
US (1) US20110287518A1 (enExample)
EP (1) EP2373677B1 (enExample)
JP (1) JP5788803B2 (enExample)
KR (1) KR101821143B1 (enExample)
CN (1) CN102239175A (enExample)
AU (1) AU2009324204B2 (enExample)
BR (1) BRPI0922716A2 (enExample)
CA (1) CA2742246A1 (enExample)
ES (1) ES2576109T3 (enExample)
MX (1) MX2011005474A (enExample)
RU (1) RU2544860C2 (enExample)
WO (1) WO2010063717A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201407983VA (en) * 2009-12-18 2015-01-29 Csl Ltd Method of purifying polypeptides
EP2526115B1 (en) 2010-01-18 2018-08-01 Novo Nordisk Health Care AG Purification of blood coagulation factors
CN107915769A (zh) * 2011-03-29 2018-04-17 葛兰素史密斯克莱有限责任公司 用于蛋白纯化的缓冲液体系
CA2838814A1 (en) 2011-08-25 2013-02-28 F.Hoffmann-La Roche Ag Cation and anion exchange chromatography method
EP3262409B1 (en) * 2015-02-25 2020-03-25 Omrix Biopharmaceuticals Ltd. Method for purifying and quantifying thrombin and its degradation polypeptides
WO2016207328A1 (en) * 2015-06-24 2016-12-29 Glycotope Gmbh PROCESS FOR THE PURIFICATION OF γ-CARBOXYLATED POLYPEPTIDES
CA3027143A1 (en) * 2016-07-15 2018-01-18 F. Hoffmann-La Roche Ag Method for purifying pegylated erythropoietin
KR20220079844A (ko) * 2019-10-14 2022-06-14 피어스 바이오테크놀로지, 인크 펩티드 정제 제제 및 방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
KR101204712B1 (ko) * 2003-03-18 2012-11-27 노보 노르디스크 헬스 케어 악티엔게젤샤프트 Ｇｌａ-잔기 함유 세린 프로테아제의 제조 방법
JP2008525381A (ja) * 2004-12-23 2008-07-17 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少
EP2125866B1 (en) * 2007-02-28 2013-05-22 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules

Also Published As

Publication number Publication date
KR101821143B1 (ko) 2018-01-23
AU2009324204B2 (en) 2014-10-16
BRPI0922716A2 (pt) 2016-11-01
MX2011005474A (es) 2011-06-16
RU2011125656A (ru) 2013-01-10
JP2012510500A (ja) 2012-05-10
US20110287518A1 (en) 2011-11-24
EP2373677A1 (en) 2011-10-12
RU2544860C2 (ru) 2015-03-20
CA2742246A1 (en) 2010-06-10
AU2009324204A1 (en) 2010-06-10
ES2576109T3 (es) 2016-07-05
EP2373677B1 (en) 2016-03-23
WO2010063717A1 (en) 2010-06-10
CN102239175A (zh) 2011-11-09
KR20110099251A (ko) 2011-09-07

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