JP2011518564A - 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 - Google Patents

改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 Download PDF

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JP2011518564A
JP2011518564A JP2011506460A JP2011506460A JP2011518564A JP 2011518564 A JP2011518564 A JP 2011518564A JP 2011506460 A JP2011506460 A JP 2011506460A JP 2011506460 A JP2011506460 A JP 2011506460A JP 2011518564 A JP2011518564 A JP 2011518564A
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isoprene
isoprene synthase
variant
host cell
seq
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JP2011518564A5 (enExample
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セルバン、マルグリッテ・アー
ホワイテッド、グレゴリー・エム
ミアスニコフ、アンドレイ
ペレス、カロリーン・エム
ワイラー、ウォルター
ウェルス、デレック・エイチ
ボット、リチャード・アール
ライフ、クリストファー・リー
マカリフィー、ジョゼフ・シー
ケリス、ジェイムズ
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Goodyear Tire and Rubber Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)

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  • Enzymes And Modification Thereof (AREA)
JP2011506460A 2008-04-23 2009-04-23 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 Pending JP2011518564A (ja)

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US12533608P 2008-04-23 2008-04-23
US61/125,336 2008-04-23
PCT/US2009/041581 WO2009132220A2 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene

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JP2014085377A Pending JP2014158489A (ja) 2008-04-23 2014-04-17 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体

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KR (1) KR20110020234A (enExample)
CN (1) CN102171335A (enExample)
AU (1) AU2009240505B2 (enExample)
BR (1) BRPI0911667A2 (enExample)
CA (1) CA2722440A1 (enExample)
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Cited By (10)

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JP2011526943A (ja) * 2008-06-30 2011-10-20 ザ・グッドイヤー・タイヤ・アンド・ラバー・カンパニー 再生資源に由来するイソプレンの重合体
WO2013179722A1 (ja) 2012-05-30 2013-12-05 株式会社ブリヂストン イソプレンシンターゼおよびそれをコードするポリヌクレオチド、ならびにイソプレンモノマーの製造方法
WO2014065271A1 (ja) 2012-10-23 2014-05-01 積水化学工業株式会社 組換え細胞、並びに、イソプレンの生産方法
JP2015503904A (ja) * 2011-10-27 2015-02-05 ダニスコ・ユーエス・インク イソプレン産生時の溶解度が向上されたイソプレン合成酵素変異体
US8962296B2 (en) 2012-05-30 2015-02-24 Bridgestone Corporation Isoprene synthase and gene encoding the same, and method for producing isoprene monomer
WO2015076392A1 (ja) 2013-11-22 2015-05-28 味の素株式会社 改変イソプレンシンターゼ
WO2015080273A1 (ja) 2013-11-28 2015-06-04 味の素株式会社 イソプレンモノマーの製造方法
WO2015147341A2 (en) 2014-03-28 2015-10-01 Ajinomoto Co., Inc. Method of producing isoprene monomer
WO2018155272A1 (ja) 2017-02-27 2018-08-30 積水化学工業株式会社 組換え細胞、組換え細胞の製造方法、並びに、イソプレン又はテルペンの生産方法
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation

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