CA2722440A1 - Isoprene synthase variants for improved microbial production of isoprene - Google Patents

Isoprene synthase variants for improved microbial production of isoprene Download PDF

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Publication number
CA2722440A1
CA2722440A1 CA2722440A CA2722440A CA2722440A1 CA 2722440 A1 CA2722440 A1 CA 2722440A1 CA 2722440 A CA2722440 A CA 2722440A CA 2722440 A CA2722440 A CA 2722440A CA 2722440 A1 CA2722440 A1 CA 2722440A1
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CA
Canada
Prior art keywords
variant
isoprene
isoprene synthase
seq
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2722440A
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English (en)
French (fr)
Inventor
Marguerite A. Cervin
Gregory M. Whited
Andrei Miasnikov
Caroline M. Peres
Walter Weyler
Derek H. Wells
Richard R. Bott
Christopher Lee. Rife
James Kellis
Joseph C. Mcauliffe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Goodyear Tire and Rubber Co
Danisco US Inc
Original Assignee
Goodyear Tire and Rubber Co
Danisco US Inc
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Application filed by Goodyear Tire and Rubber Co, Danisco US Inc filed Critical Goodyear Tire and Rubber Co
Publication of CA2722440A1 publication Critical patent/CA2722440A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/03Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027Isoprene synthase (4.2.3.27)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2722440A 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene Abandoned CA2722440A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12533608P 2008-04-23 2008-04-23
US61/125,336 2008-04-23
PCT/US2009/041581 WO2009132220A2 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene

Publications (1)

Publication Number Publication Date
CA2722440A1 true CA2722440A1 (en) 2009-10-29

Family

ID=40810540

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2722440A Abandoned CA2722440A1 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene

Country Status (14)

Country Link
US (3) US8173410B2 (enExample)
EP (1) EP2279251A2 (enExample)
JP (2) JP2011518564A (enExample)
KR (1) KR20110020234A (enExample)
CN (1) CN102171335A (enExample)
AU (1) AU2009240505B2 (enExample)
BR (1) BRPI0911667A2 (enExample)
CA (1) CA2722440A1 (enExample)
MX (1) MX2010011706A (enExample)
MY (1) MY153871A (enExample)
RU (2) RU2516343C2 (enExample)
SG (2) SG192545A1 (enExample)
WO (1) WO2009132220A2 (enExample)
ZA (2) ZA201007564B (enExample)

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