JP2010502197A5 - - Google Patents

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JP2010502197A5
JP2010502197A5 JP2009526931A JP2009526931A JP2010502197A5 JP 2010502197 A5 JP2010502197 A5 JP 2010502197A5 JP 2009526931 A JP2009526931 A JP 2009526931A JP 2009526931 A JP2009526931 A JP 2009526931A JP 2010502197 A5 JP2010502197 A5 JP 2010502197A5
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plant
corn
corn plant
cells
regenerated
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JP2010502197A (ja
JP5329412B2 (ja
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Priority claimed from PCT/US2007/077370 external-priority patent/WO2008028121A1/en
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JP2009526931A 2006-08-31 2007-08-31 選択を含まない植物形質転換 Active JP5329412B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US84151906P 2006-08-31 2006-08-31
US60/841,519 2006-08-31
PCT/US2007/077370 WO2008028121A1 (en) 2006-08-31 2007-08-31 Plant transformation without selection

Publications (3)

Publication Number Publication Date
JP2010502197A JP2010502197A (ja) 2010-01-28
JP2010502197A5 true JP2010502197A5 (enExample) 2010-09-30
JP5329412B2 JP5329412B2 (ja) 2013-10-30

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JP2009526930A Active JP5242570B2 (ja) 2006-08-31 2007-08-31 単子葉を急速に形質転換する方法
JP2009526931A Active JP5329412B2 (ja) 2006-08-31 2007-08-31 選択を含まない植物形質転換
JP2009526926A Active JP5350246B2 (ja) 2006-08-31 2007-08-31 トランスジェニック植物を作製する方法

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JP2009526926A Active JP5350246B2 (ja) 2006-08-31 2007-08-31 トランスジェニック植物を作製する方法

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US (16) US8395020B2 (enExample)
EP (5) EP2069510B1 (enExample)
JP (3) JP5242570B2 (enExample)
CN (5) CN104232680B (enExample)
AT (1) ATE497008T1 (enExample)
AU (3) AU2007289105B2 (enExample)
BR (4) BRPI0716207B1 (enExample)
CA (8) CA2661825C (enExample)
DE (1) DE602007012269D1 (enExample)
MX (3) MX2009002252A (enExample)
WO (3) WO2008028119A2 (enExample)
ZA (3) ZA200901140B (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040210958A1 (en) * 2003-03-19 2004-10-21 Monsanto Technology Llc A Novel Culture Method for Corn Transformation
US7682829B2 (en) * 2003-05-30 2010-03-23 Monsanto Technology Llc Methods for corn transformation
JP4525314B2 (ja) * 2004-11-26 2010-08-18 沖電気工業株式会社 光符号分割多重送受信方法及び光符号分割多重送受信装置
US8395020B2 (en) * 2006-08-31 2013-03-12 Monsanto Technology Llc Methods for rapidly transforming monocots
WO2009029852A2 (en) 2007-08-31 2009-03-05 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
JP2011120478A (ja) * 2008-03-31 2011-06-23 Japan Tobacco Inc アグロバクテリウム菌による形質転換植物の作成方法
CA2766075A1 (en) 2009-06-25 2010-12-29 Syngenta Participations Ag Methods for agrobacterium-mediated transformation of sugar cane
CA2773281C (en) * 2009-09-11 2020-01-07 Imperial Innovations Limited Method of producing leafy biomass from undifferentiated plant cells, and related method for polypeptide production
WO2011163292A1 (en) 2010-06-24 2011-12-29 Syngenta Participations Ag Methods for agrobacterium-mediated transformation of sugar cane
EP4059342A1 (en) 2010-12-17 2022-09-21 Monsanto Technology LLC Methods for improving competency of plant cells
US20130091606A1 (en) * 2011-06-24 2013-04-11 Bayer Cropscience Lp Methods and compositions for enhanced Agrobacterium-mediated transformation Efficiency
US11807846B2 (en) * 2012-04-29 2023-11-07 Monsanto Technology Llc Methods for transforming corn explants
US11713465B2 (en) 2013-11-21 2023-08-01 Monsanto Technology, Llc Methods for transforming wheat explants and compositions therefor
DE102013020605A1 (de) 2013-12-15 2015-06-18 Kws Saat Ag Selektionsmarker-freies rhizobiaceae-vermitteltes verfahren zur herstellung einer transgenen pflanze der gattung triticum
CN103766221B (zh) * 2014-02-10 2016-08-17 中国农业大学 一种单子叶植物的组织培养方法
AR101066A1 (es) 2014-03-07 2016-11-23 Pioneer Hi Bred Int Método y disposición para extraer gérmenes de vegetales monocotiledóneos
GB2533129A (en) * 2014-12-11 2016-06-15 Alkion Biopharma Sas Bioreactor
WO2016105185A1 (en) * 2014-12-22 2016-06-30 Keygene N.V. Plant callus populations
JP2018521648A (ja) * 2015-06-29 2018-08-09 ダウ アグロサイエンシィズ エルエルシー 自動化された外植片調製のためのシステム及び使用方法
JP6716876B2 (ja) * 2015-09-14 2020-07-01 住友ゴム工業株式会社 遺伝的に改変された植物体を作製するための形質転換植物体の製造方法、及びこの製造方法により作製された、遺伝的に改変された形質転換植物体
ES2881392T3 (es) 2015-10-30 2021-11-29 Pioneer Hi Bred Int Métodos y composiciones para transformación de plantas rápida
CN105660422B (zh) * 2016-04-20 2017-09-29 遵义医学院 一种白及种苗的组织培养方法
EP3400962A1 (en) 2017-05-09 2018-11-14 Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. Inhibitors of il-38 for use in treating and/or preventing cancer in a subject
WO2018220181A1 (en) * 2017-06-02 2018-12-06 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for automated transformation of a plant cell pack
CN110226517B (zh) * 2019-06-26 2021-06-01 北京市农林科学院 一种洋葱离体再生的方法及其使用的培养基
CN110607323A (zh) * 2019-09-24 2019-12-24 四川育良生物科技有限公司 一种农杆菌介导水稻遗传转化方法
WO2023049893A1 (en) * 2021-09-27 2023-03-30 Monsanto Technology Llc Compositions and methods for transformation of monocot seed excised embryo explants
WO2023049901A1 (en) * 2021-09-27 2023-03-30 Monsanto Technology Llc Compositions and methods for transformation of monocot seed excised embryo explants
AR127146A1 (es) * 2021-09-27 2023-12-20 Monsanto Technology Llc Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneas
US20240301435A1 (en) * 2021-09-27 2024-09-12 Monsanto Technology Llc Compositions and methods for transformation of monocot seed excised embryo explants
EP4451853A1 (en) * 2021-12-22 2024-10-30 Basf Agricultural Solutions Seed Us Llc Improved screening method for genome edited events
AR128429A1 (es) * 2022-02-03 2024-05-08 Corteva Agriscience Llc Preparación de explantes foliares de monocotiledóneas
US20240376485A1 (en) * 2023-05-11 2024-11-14 Wisconsin Alumni Research Foundation Meristem transformation method using a liquid selection medium

Family Cites Families (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1470850A (en) 1972-12-01 1977-04-21 Allied Chem Moulding poder
US3847888A (en) 1972-12-01 1974-11-12 Allied Chem Ultra-high molecular weight polyethylene molding powder and molding process
WO1983001176A1 (en) 1981-10-01 1983-04-14 Int Plant Research Inst Process for the genetic modification of cereals with transformation vectors
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US4757011A (en) 1983-09-30 1988-07-12 E. I. Du Pont De Nemours And Company Herbicide resistant tobacco
WO1985001856A1 (en) 1983-11-03 1985-05-09 Johannes Martenis Jacob De Wet Method for the transfer of exogenous genes in plants using pollen as a vector
DE3405185C2 (de) 1984-02-14 1987-02-26 Plüss-Staufer AG, Oftringen, Aargau Vorrichtung zur Herstellung von Kompaktlingen aus Polymeren und Additiven
EP0175769B1 (en) 1984-03-29 1990-10-31 Hoechst Celanese Corporation Polyethylene molding composition and process
NL8401048A (nl) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten.
ATE204017T1 (de) 1984-05-11 2001-08-15 Syngenta Participations Ag Transformation von pflanzenerbgut
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US5004863B2 (en) * 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US4971908A (en) 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
ATE126005T1 (de) 1988-04-25 1995-08-15 Monsanto Co Insektenresistente salatpflanzen.
WO1989012102A1 (en) 1988-06-01 1989-12-14 The Texas A&M University System Method for transforming plants via the shoot apex
US5990387A (en) * 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
EP1103616A3 (en) 1989-02-24 2001-06-27 Monsanto Company Synthetic plant genes and method for preparation
EP0388186A1 (en) * 1989-03-17 1990-09-19 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5106739A (en) 1989-04-18 1992-04-21 Calgene, Inc. CaMv 355 enhanced mannopine synthase promoter and method for using same
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5225341A (en) 1990-07-19 1993-07-06 The Regents Of The University Of California Biologically safe plant transformation system using a ds transposon
ATE318318T1 (de) * 1990-11-23 2006-03-15 Bayer Bioscience Nv Verfahren zur transformation monokotyler pflanzen
US5484620A (en) 1990-12-22 1996-01-16 Schenk-Filterbau Gesellschaft Mit Beschrankter Haftung Method of manufacturing stabilizing and/or filtering aids for use in the processing of liquids, especially beverages
AU662202B2 (en) 1992-02-24 1995-08-24 Montell North America Inc. Polyolefin compositions having good transparency and impact resistance
DE4210351A1 (de) 1992-03-30 1993-10-07 Hoechst Ag Verfahren zum Pelletieren von ultrahochmolekularem Polyethylen
DE4211529A1 (de) 1992-04-06 1993-10-07 Herding Entstaubung Filterelement mit einem formstabilen, durchlässig-porösen Kunststoff-Formkörper
DE69334225D1 (de) * 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
GB9321557D0 (en) * 1992-11-03 1993-12-08 Zeneca Ltd Carboxamide derivatives
FI933682A0 (fi) 1993-08-20 1993-08-20 Valtion Teknillinen Metod foer att producera transgena saedesvaexter
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
DE69433809T2 (de) 1993-09-03 2005-06-16 Japan Tobacco Inc. Verfahren zur transformation von monocotyledonen mittels des schildes von unreifen embryonen
JP3102888B2 (ja) 1993-12-08 2000-10-23 日本たばこ産業株式会社 植物の形質転換方法及びそのためのベクター
US5750870A (en) 1994-06-17 1998-05-12 Agritope, Inc. Plant genetic transformation methods and transgenic plants
US5767368A (en) 1995-04-03 1998-06-16 Board Of Trustees Operating Michigan State University Method for producting a cereal plant with foreign DNA
DE19517465C1 (de) * 1995-05-12 1997-01-23 Degussa Percarbonsäurelösungen mit verbesserter Stabilität im Kontakt mit Edelstahl
AR006928A1 (es) * 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
EP0856060A2 (en) 1996-06-21 1998-08-05 Monsanto Company METHODS FOR THE PRODUCTION OF STABLY-TRANSFORMED, FERTILE WHEAT EMPLOYING $i(AGROBACTERIUM)-MEDIATED TRANSFORMATION AND COMPOSITIONS DERIVED THEREFROM
CN1206435A (zh) * 1996-10-22 1999-01-27 日本烟草产业株式会社 印度水稻的转化方法
JPH10117776A (ja) * 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
US5919999A (en) 1996-11-14 1999-07-06 Queen's University At Kingston Enhanced transport with a plastid membrane transport protein
US6002068A (en) * 1996-12-19 1999-12-14 Novartis Finance Corporation Methods for conferring insect resistance to a monocot using a perioxidase coding sequence
US5981840A (en) * 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
AU7479298A (en) 1997-05-16 1998-12-08 Pioneer Hi-Bred International, Inc. Recovery of transformed plants without selectable markers by nodal culture and enrichment of transgenic sectors
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
US6140555A (en) * 1997-06-06 2000-10-31 Mississippi State University Methods for maize transformation coupled with adventitious regeneration utilizing nodal section explants and mature zygotic embryos
FI104907B (fi) * 1997-09-18 2000-04-28 Univ Helsinki Licensing Rypsin Agrobacterium-välitteinen transformaatio
SG70124A1 (en) 1997-09-26 2000-01-25 Gen Electric Method for making thermoplastic resin pellets
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6043410A (en) 1998-02-06 2000-03-28 Calgene Llc Strawberry fruit promoters for gene expression
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
AU760041B2 (en) 1998-04-08 2003-05-08 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
AR020078A1 (es) 1998-05-26 2002-04-10 Syngenta Participations Ag Metodo para alterar la expresion de un gen objetivo en una celula de planta
JP2000083680A (ja) 1998-07-16 2000-03-28 Nippon Paper Industries Co Ltd 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ―
ATE313635T1 (de) 1998-10-01 2006-01-15 Pioneer Hi Bred Int Methode zur pflanzentransformation
US6825397B1 (en) * 1998-11-09 2004-11-30 Pioneer Hi-Bred International, Inc. LEC1 trancriptional activator nucleic acids and methods of use thereof
DE69941009D1 (de) * 1998-11-17 2009-07-30 Monsanto Technology Llc Phosphonat metabolisierende pflanzen
US6420630B1 (en) * 1998-12-01 2002-07-16 Stine Biotechnology Methods for tissue culturing and transforming elite inbreds of Zea mays L.
US6846970B1 (en) 1999-07-19 2005-01-25 Plant Bioscience Limited Transformation method and transgenic plants produced thereby
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
EP1111063A1 (en) 1999-12-24 2001-06-27 Coöperatieve Verkoop- en Productievereniging van Aardappelmeel en Derivaten 'AVEBE' B.V. Modified DHPS genes
AU2001245359A1 (en) 2000-03-02 2001-09-12 Auburn University Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
DE60142470D1 (de) 2000-04-24 2010-08-12 Vertex Pharma Verfahren und zwischenprodukte zur herstellung von substituierten asparaginsäure-acetalen
US6492174B1 (en) * 2000-06-19 2002-12-10 Institute Of Paper Science & Technology Methods of initiating embryogenic cultures in plants
AU8980001A (en) * 2000-08-11 2002-02-25 Syngenta Participations Ag Methods for stable transformation of plants
US6995016B2 (en) 2000-08-17 2006-02-07 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Process for inducing direct somatic embryogenesis in immature scutella cells of pooideae, and rapidly regenerating fertile plants
AUPR143100A0 (en) * 2000-11-10 2000-12-07 Bureau Of Sugar Experiment Stations Plant transformation
CN1356389A (zh) * 2000-12-08 2002-07-03 大连理工大学 一种无选择标记的植物转基因方法
US7098383B2 (en) 2000-12-19 2006-08-29 Sembiosys Genetics Inc. Methods for the production of multimeric immunoglobulins, and related compositions
DE10101276A1 (de) 2001-01-12 2002-07-18 Icon Genetics Ag Verfahren und Vektoren zur Transformation von Plastiden
US20040237133A1 (en) * 2001-06-15 2004-11-25 Goldman Stephen L. Method for transformation of mono-and di-cotyledonous plants using meristematic tissue and nodal callus from dicotyledonous plants
US20030082580A1 (en) * 2001-07-06 2003-05-01 Dna Plant Technology Corporation Plant cell culture and selection system
US20030115641A1 (en) 2001-07-24 2003-06-19 Dobres Michael S. Transformation of plants by electroporation of cultured explants
JP2003047463A (ja) 2001-07-24 2003-02-18 Kumho Petrochem Co Ltd シバ成熟種子由来カルスのための植物体再生方法
EP1279737A1 (en) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformation method for obtaining marker-free plants
US20030135891A1 (en) 2001-12-04 2003-07-17 The Texas A&M University System Method of transforming intact plants
CN102978236B (zh) * 2002-02-20 2015-09-09 J·R·西姆普罗特公司 精确育种
US20030196219A1 (en) 2002-04-16 2003-10-16 Monsanto Technology Llc Novel Method for Agrobacterium Preparation for Plant Transformation
WO2004022707A2 (en) * 2002-09-03 2004-03-18 The Regents Of The University Of California Method and compositions for transformation and regeneration of maize
PT1589807E (pt) * 2002-12-06 2012-02-02 Del Monte Fresh Produce Company Plantas transgénicas de ananás com níveis modificados de carotenóides e métodos da sua produção
WO2004081184A2 (en) 2003-03-07 2004-09-23 Seminis Vegetable Seeds, Inc. Markerless transformation
US20040210959A1 (en) * 2003-03-19 2004-10-21 Monsanto Technology Llc A Novel Method for Production of Transformed Dihaploid Corn Plants
US20040210958A1 (en) 2003-03-19 2004-10-21 Monsanto Technology Llc A Novel Culture Method for Corn Transformation
US7575917B2 (en) 2003-04-09 2009-08-18 Monsanto Technology Llc DNA constructs and methods to enhance the production of commercially viable transgenic plants
US7682829B2 (en) 2003-05-30 2010-03-23 Monsanto Technology Llc Methods for corn transformation
ATE405660T1 (de) 2003-06-17 2008-09-15 Sembiosys Genetics Inc Verfahren zur insulinproduktion in pflanzen
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
TW200526778A (en) 2003-11-14 2005-08-16 Sembiosys Genetics Inc Methods for the production of apolipoproteins in transgenic plants
ES2384876T3 (es) 2004-04-20 2012-07-13 Temasek Life Sciences Laboratory Limited Procedimiento de transformación y regeneración altamente eficiente de células vegetales en suspensión
US7572635B2 (en) 2004-06-25 2009-08-11 Monsanto Technology Llc Method for agrobacterium transformation for dihaploid corn plants
US8395020B2 (en) * 2006-08-31 2013-03-12 Monsanto Technology Llc Methods for rapidly transforming monocots
US8058514B2 (en) 2007-02-28 2011-11-15 Japan Tobacco Inc. Agrobacterium-mediated method for producing transformed plant without selection step
EP2143273A4 (en) 2007-04-02 2012-08-08 Esight Corp DEVICE AND METHOD FOR SUPPRESSION
WO2009029852A2 (en) 2007-08-31 2009-03-05 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
MY145205A (en) 2007-12-28 2012-01-13 Mimos Berhad Vibratory sensor
JP2011120478A (ja) 2008-03-31 2011-06-23 Japan Tobacco Inc アグロバクテリウム菌による形質転換植物の作成方法
EP2110528B2 (en) 2008-04-18 2017-04-19 Daimler AG Apparatus for treating an exhaust gas stream with removable module
JP5615539B2 (ja) 2009-12-08 2014-10-29 株式会社タイヨー製作所 連続式加熱処理方法及び連続式加熱処理装置
US20110162112A1 (en) * 2009-12-31 2011-06-30 Pioneer Hi-Bred International, Inc. Increasing time-efficiency of high-throughput transformation processes

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