JP2007521823A - プロテアーゼ変異型 - Google Patents

プロテアーゼ変異型 Download PDF

Info

Publication number
JP2007521823A
JP2007521823A JP2006552463A JP2006552463A JP2007521823A JP 2007521823 A JP2007521823 A JP 2007521823A JP 2006552463 A JP2006552463 A JP 2006552463A JP 2006552463 A JP2006552463 A JP 2006552463A JP 2007521823 A JP2007521823 A JP 2007521823A
Authority
JP
Japan
Prior art keywords
protease
amino acid
blc
parent
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2006552463A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007521823A5 (https=
Inventor
スベンセン,アラン
ミニング,ステファン
Original Assignee
ノボザイムス アクティーゼルスカブ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ノボザイムス アクティーゼルスカブ filed Critical ノボザイムス アクティーゼルスカブ
Publication of JP2007521823A publication Critical patent/JP2007521823A/ja
Publication of JP2007521823A5 publication Critical patent/JP2007521823A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2299/00Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Treatment And Processing Of Natural Fur Or Leather (AREA)
JP2006552463A 2004-02-13 2005-02-14 プロテアーゼ変異型 Pending JP2007521823A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200400226 2004-02-13
PCT/DK2005/000097 WO2005078074A2 (en) 2004-02-13 2005-02-14 Protease variants

Publications (2)

Publication Number Publication Date
JP2007521823A true JP2007521823A (ja) 2007-08-09
JP2007521823A5 JP2007521823A5 (https=) 2008-04-10

Family

ID=34854485

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006552463A Pending JP2007521823A (ja) 2004-02-13 2005-02-14 プロテアーゼ変異型

Country Status (5)

Country Link
US (2) US20070281332A1 (https=)
EP (3) EP2325318A1 (https=)
JP (1) JP2007521823A (https=)
CN (2) CN102250861A (https=)
WO (1) WO2005078074A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016528894A (ja) * 2013-07-26 2016-09-23 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲノムエンジニアリング

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001016285A2 (en) * 1999-08-31 2001-03-08 Novozymes A/S Novel proteases and variants thereof
JP5396270B2 (ja) * 2006-06-02 2014-01-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ タンパク質表面のリモデリング
EP2030015B1 (en) 2006-06-02 2016-02-17 President and Fellows of Harvard College Protein surface remodeling
NZ577510A (en) * 2006-12-20 2012-06-29 Danisco Milk protein hydrolyzates with reduced immunogenic potential
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
AU2009240505B2 (en) 2008-04-23 2013-09-05 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
AU2010238770A1 (en) * 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
EP2424877A4 (en) 2009-04-28 2013-01-02 Harvard College SUPRAGELADENE PROTEINS FOR CELL PENETRATION
CA2816306A1 (en) 2010-10-27 2012-05-03 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
CN105358686A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
WO2015014804A1 (en) * 2013-07-29 2015-02-05 Novozymes A/S Protease variants and polynucleotides encoding same
US10402358B2 (en) * 2014-09-30 2019-09-03 Honeywell International Inc. Module auto addressing in platform bus
CN104561075A (zh) * 2014-11-13 2015-04-29 广东省微生物研究所 一种重组表达谷氨酸特异性内肽酶的地衣芽孢杆菌及其构建方法
CA3088793A1 (en) 2018-02-06 2019-08-15 Novozymes Bioag A/S Methods of protecting a plant from fungal pests
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN121420051A (zh) * 2023-07-07 2026-01-27 诺维信公司 用于去除蛋白质污渍的洗涤方法
WO2026017381A1 (en) 2024-07-17 2026-01-22 Novozymes A/S Optimized bacillus host cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04166085A (ja) * 1990-10-24 1992-06-11 Shionogi & Co Ltd 新規プロテアーゼ
WO2001016285A2 (en) * 1999-08-31 2001-03-08 Novozymes A/S Novel proteases and variants thereof

Family Cites Families (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US53711A (en) 1866-04-03 Improvement in the construction of pulleys
GB1296839A (https=) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
FR2430453B1 (fr) 1978-07-04 1986-04-18 Novo Industri As Produit proteasique a pouvoir allergisant reduit
US4288627A (en) 1980-02-12 1981-09-08 Phillips Petroleum Company Oxidation of thiols employing cobalt molybdate/triethylamine catalyst
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
US4599311A (en) 1982-08-13 1986-07-08 Kawasaki Glenn H Glycolytic promotersfor regulated protein expression: protease inhibitor
US4745051A (en) 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
US4879236A (en) 1984-05-16 1989-11-07 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4775624A (en) 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
AU607690B2 (en) 1985-12-24 1991-03-14 Marion Laboratories, Inc. Use of synthetic sulfated saccharides to enhance wound healing
US4935349A (en) 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DE3750450T2 (de) 1986-08-29 1995-01-05 Novo Industri As Enzymhaltiger Reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE3856593D1 (de) * 1987-04-06 2007-10-04 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-Bindungsstellen zur Stabilisierung von Proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
CA1309680C (en) 1987-07-24 1992-11-03 David Harano Airlift insect cell culture
US5024947A (en) 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
DE68924654T2 (de) 1988-01-07 1996-04-04 Novonordisk As Spezifische Protease.
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
US5118623A (en) 1988-05-27 1992-06-02 Solvay Enzymes, Inc. Bleach stable enzymes
CA2003078A1 (en) 1988-11-18 1990-05-18 Biotechnica International, Inc. Protease deletion
GB8910962D0 (en) 1989-05-12 1989-06-28 Natural Environment Res Novel baculovirus expression vectors and use thereof in the expression of foreign proteins in insects or insect cells
DK316989D0 (da) 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US5162222A (en) 1989-07-07 1992-11-10 Guarino Linda A Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells or recombinant baculoviruses
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5077214A (en) 1989-07-07 1991-12-31 The Texas A&M University System Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
US5155037A (en) 1989-08-04 1992-10-13 The Texas A&M University System Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
DK63490D0 (da) 1990-03-09 1990-03-09 Novo Nordisk As Fremgangsmaade til fremstilling af ost
KR100236540B1 (ko) 1990-04-14 2000-01-15 레클로우크스 라우에르 알카리성 바실러스-리파제, 이를 코-딩하는 dna 서열 및 리파제를 생산하는 바실러스 균주
DK0531372T4 (da) 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
AU657278B2 (en) 1990-09-13 1995-03-09 Novo Nordisk A/S Lipase variants
ES2174820T3 (es) 1991-01-16 2002-11-16 Procter & Gamble Composiciones detergentes compactas con celulasa de alta actividad.
CZ285148B6 (cs) 1991-04-30 1999-05-12 The Procter And Gamble Company Kapalné detergentní směsi s borito-polyolovým komplexem k inhibici proteolytického enzymu
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
ES2121014T3 (es) 1991-05-01 1998-11-16 Novo Nordisk As Enzimas estabilizadas y composiciones detergentes.
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
WO1994002597A1 (en) 1992-07-23 1994-02-03 Novo Nordisk A/S MUTANT α-AMYLASE, DETERGENT, DISH WASHING AGENT, AND LIQUEFACTION AGENT
ATE262035T1 (de) 1992-10-06 2004-04-15 Novozymes As Zellulosevarianten
PT867504E (pt) 1993-02-11 2003-08-29 Genencor Int Alfa-amilase estavel a oxidacao
KR950702240A (ko) 1993-04-27 1995-06-19 한스 발터 라벤 세제로의 이용을 위한 새로운 리파제 변형체
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
US5817495A (en) 1993-10-13 1998-10-06 Novo Nordisk A/S H2 O2 -stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
DE69527835T2 (de) 1994-02-22 2003-04-10 Novozymes A/S, Bagsvaerd Methode zur herstellung einer variante eines lipolytischen enzymes
DE69536145D1 (de) 1994-03-08 2011-04-07 Novozymes As Neuartige alkalische Zellulasen
WO1995030744A2 (en) 1994-05-04 1995-11-16 Genencor International Inc. Lipases with improved surfactant resistance
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
AU2884695A (en) 1994-06-23 1996-01-19 Unilever Plc Modified pseudomonas lipases and their use
DE69535733T2 (de) 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
US5827719A (en) 1994-10-26 1998-10-27 Novo Nordisk A/S Enzyme with lipolytic activity
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
CN102080070B (zh) 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 新的内切葡聚糖酶
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
ATE429490T1 (de) 1995-05-05 2009-05-15 Novozymes As Protease-varianten und verbindungen
EP0839186B1 (en) 1995-07-14 2004-11-10 Novozymes A/S A modified enzyme with lipolytic activity
AU6655196A (en) 1995-08-11 1997-03-12 Novo Nordisk A/S Novel lipolytic enzymes
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
EP1726644A1 (en) * 1996-09-17 2006-11-29 Novozymes A/S Cellulase variants
WO1998015257A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
EP0932667B1 (en) 1996-11-04 2008-10-01 Novozymes A/S Subtilase variants and compositions
JP4044143B2 (ja) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ ズブチラーゼ変異体及び組成物
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
AR015977A1 (es) 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US6558939B1 (en) * 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
AU2002223020B2 (en) 2000-11-27 2006-07-06 Novozymes A/S Automated mechanical stress assay for screening cleaning ingredients
EP1995319B1 (en) * 2002-04-10 2011-08-31 Novozymes A/S Bacillus licheniformis mutant host cell

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04166085A (ja) * 1990-10-24 1992-06-11 Shionogi & Co Ltd 新規プロテアーゼ
WO2001016285A2 (en) * 1999-08-31 2001-03-08 Novozymes A/S Novel proteases and variants thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016528894A (ja) * 2013-07-26 2016-09-23 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲノムエンジニアリング
US10563225B2 (en) 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
JP2020110152A (ja) * 2013-07-26 2020-07-27 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲノムエンジニアリング
JP6993063B2 (ja) 2013-07-26 2022-01-13 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲノムエンジニアリング
US11306328B2 (en) 2013-07-26 2022-04-19 President And Fellows Of Harvard College Genome engineering
US12473575B2 (en) 2013-07-26 2025-11-18 President And Fellows Of Harvard College Genome engineering

Also Published As

Publication number Publication date
EP2305821A2 (en) 2011-04-06
US20100196990A1 (en) 2010-08-05
EP1713919A2 (en) 2006-10-25
EP2305821A3 (en) 2011-04-13
CN102250861A (zh) 2011-11-23
US8563289B2 (en) 2013-10-22
EP2325318A1 (en) 2011-05-25
WO2005078074A2 (en) 2005-08-25
CN1942584A (zh) 2007-04-04
US20070281332A1 (en) 2007-12-06
WO2005078074A3 (en) 2005-11-03
CN1942584B (zh) 2011-07-27

Similar Documents

Publication Publication Date Title
US8563289B2 (en) Protease variants
US10144922B2 (en) Subtilase variants and compositions comprising the same
US8008057B2 (en) Subtilases
JP2012045000A (ja) サブチラーゼ
KR101293498B1 (ko) 서브틸라제 변이체
US7507569B2 (en) Variant subtilisin enzymes (subtilases)
EP1590454A2 (en) Subtilases
US20040147008A1 (en) Subtilase variants
CN1166183A (zh) 无钙的枯草杆菌蛋白酶突变体
CN1768137A (zh) 枯草杆菌酶
EP1914307A2 (en) Subtilases
WO2005010176A1 (en) Method for making polypeptide variants by combinatorial fragment exchange

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080213

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20080213

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110118

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110413

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110420

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110719

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20110920