CN102250861A - 蛋白酶变体 - Google Patents

蛋白酶变体 Download PDF

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Publication number
CN102250861A
CN102250861A CN2011101376244A CN201110137624A CN102250861A CN 102250861 A CN102250861 A CN 102250861A CN 2011101376244 A CN2011101376244 A CN 2011101376244A CN 201110137624 A CN201110137624 A CN 201110137624A CN 102250861 A CN102250861 A CN 102250861A
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CN
China
Prior art keywords
protease
positions
blc
amino acid
variant
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN2011101376244A
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English (en)
Chinese (zh)
Inventor
A.斯文森
S.米宁
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Novo Nordisk AS
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Novo Nordisk AS
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Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of CN102250861A publication Critical patent/CN102250861A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2299/00Coordinates from 3D structures of peptides, e.g. proteins or enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Treatment And Processing Of Natural Fur Or Leather (AREA)
  • General Preparation And Processing Of Foods (AREA)
CN2011101376244A 2004-02-13 2005-02-14 蛋白酶变体 Pending CN102250861A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200400226 2004-02-13
DKPA200400226 2004-02-13

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2005800109412A Division CN1942584B (zh) 2004-02-13 2005-02-14 蛋白酶变体

Publications (1)

Publication Number Publication Date
CN102250861A true CN102250861A (zh) 2011-11-23

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Family Applications (2)

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CN2011101376244A Pending CN102250861A (zh) 2004-02-13 2005-02-14 蛋白酶变体
CN2005800109412A Expired - Fee Related CN1942584B (zh) 2004-02-13 2005-02-14 蛋白酶变体

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN2005800109412A Expired - Fee Related CN1942584B (zh) 2004-02-13 2005-02-14 蛋白酶变体

Country Status (5)

Country Link
US (2) US20070281332A1 (https=)
EP (3) EP2305821A3 (https=)
JP (1) JP2007521823A (https=)
CN (2) CN102250861A (https=)
WO (1) WO2005078074A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105358686A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
CN105358685A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2206786A1 (en) * 1999-08-31 2010-07-14 Novozymes A/S Novel proteases and variants thereof
JP5396270B2 (ja) * 2006-06-02 2014-01-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ タンパク質表面のリモデリング
EP2030015B1 (en) 2006-06-02 2016-02-17 President and Fellows of Harvard College Protein surface remodeling
EP2124603B1 (en) * 2006-12-20 2014-11-19 DuPont Nutrition Biosciences ApS Milk protein hydrolyzates with reduced immunogenic potential
CN105368764B (zh) 2007-12-13 2019-03-12 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
WO2009132220A2 (en) 2008-04-23 2009-10-29 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
AU2010238770A1 (en) * 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP2012525146A (ja) 2009-04-28 2012-10-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ 細胞透過のための過剰に荷電されたタンパク質
CA2816306A1 (en) 2010-10-27 2012-05-03 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
US10563225B2 (en) * 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
US10402358B2 (en) * 2014-09-30 2019-09-03 Honeywell International Inc. Module auto addressing in platform bus
CN104561075A (zh) * 2014-11-13 2015-04-29 广东省微生物研究所 一种重组表达谷氨酸特异性内肽酶的地衣芽孢杆菌及其构建方法
WO2019157061A2 (en) * 2018-02-06 2019-08-15 Novozymes Bioag A/S Methods of protecting a plant from fungal pests
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法
CN121420051A (zh) * 2023-07-07 2026-01-27 诺维信公司 用于去除蛋白质污渍的洗涤方法
WO2026017381A1 (en) 2024-07-17 2026-01-22 Novozymes A/S Optimized bacillus host cells

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US53711A (en) 1866-04-03 Improvement in the construction of pulleys
GB1296839A (https=) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
DE2926808A1 (de) 1978-07-04 1980-01-17 Novo Industri As Proteaseprodukt mit verringerter allergener wirkung, herstellungsverfahren und waschmittel
US4288627A (en) 1980-02-12 1981-09-08 Phillips Petroleum Company Oxidation of thiols employing cobalt molybdate/triethylamine catalyst
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
US4599311A (en) 1982-08-13 1986-07-08 Kawasaki Glenn H Glycolytic promotersfor regulated protein expression: protease inhibitor
US4745051A (en) 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
US4879236A (en) 1984-05-16 1989-11-07 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4775624A (en) 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
AU607690B2 (en) 1985-12-24 1991-03-14 Marion Laboratories, Inc. Use of synthetic sulfated saccharides to enhance wound healing
US4935349A (en) 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ATE371029T1 (de) * 1987-04-06 2007-09-15 Novozymes As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
AU2252088A (en) 1987-07-24 1989-03-01 Cetus Corporation Airlift insect cell culture
US5024947A (en) 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
EP0471265B1 (en) 1988-01-07 1995-10-25 Novo Nordisk A/S Specific protease
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
WO1989009259A1 (en) 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
US5118623A (en) 1988-05-27 1992-06-02 Solvay Enzymes, Inc. Bleach stable enzymes
CA2003078A1 (en) 1988-11-18 1990-05-18 Biotechnica International, Inc. Protease deletion
GB8910962D0 (en) 1989-05-12 1989-06-28 Natural Environment Res Novel baculovirus expression vectors and use thereof in the expression of foreign proteins in insects or insect cells
DK316989D0 (da) 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US5162222A (en) 1989-07-07 1992-11-10 Guarino Linda A Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells or recombinant baculoviruses
US5077214A (en) 1989-07-07 1991-12-31 The Texas A&M University System Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5155037A (en) 1989-08-04 1992-10-13 The Texas A&M University System Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
DK63490D0 (da) 1990-03-09 1990-03-09 Novo Nordisk As Fremgangsmaade til fremstilling af ost
EP0528828B2 (de) 1990-04-14 1997-12-03 Genencor International GmbH Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
CA2082279C (en) 1990-05-09 2007-09-04 Grethe Rasmussen Cellulase preparation comprising an endoglucanase enzyme
DE69129988T2 (de) 1990-09-13 1999-03-18 Novo Nordisk A/S, Bagsvaerd Lipase-varianten
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
DE69133035T2 (de) 1991-01-16 2003-02-13 The Procter & Gamble Company, Cincinnati Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
DK0583420T3 (da) 1991-04-30 1996-07-29 Procter & Gamble Builderholdige flydende detergenter med borsyre-polyol-kompleks til inhibering af proteolytisk enzym
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
EP0651794B1 (en) 1992-07-23 2009-09-30 Novozymes A/S MUTANT $g(a)-AMYLASE, DETERGENT AND DISH WASHING AGENT
EP1431389A3 (en) 1992-10-06 2004-06-30 Novozymes A/S Cellulase variants
CZ293163B6 (cs) 1993-02-11 2004-02-18 Genencor International, Inc. Mutanta alfa-amylázy, její použití, kódová DNA pro tuto mutantu, vektor pro expresi, hostitelské buňky, čisticí prostředek a prostředek pro zkapalnění škrobu
ATE287946T1 (de) 1993-04-27 2005-02-15 Genencor Int Neuartige lipasevarianten zur verwendung in reinigungsmitteln
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
EP0724631A1 (en) 1993-10-13 1996-08-07 Novo Nordisk A/S H 2?o 2?-stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
EP0746618B1 (en) 1994-02-22 2002-08-21 Novozymes A/S A method of preparing a variant of a lipolytic enzyme
WO1995024471A1 (en) 1994-03-08 1995-09-14 Novo Nordisk A/S Novel alkaline cellulases
JP3851656B2 (ja) 1994-05-04 2006-11-29 ジェネンコア インターナショナル インコーポレーテッド 改善された界面活性剤耐性を有するリパーゼ
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
DE69535733T2 (de) 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
BR9509525A (pt) 1994-10-26 1995-10-26 Novo Nordisk As Construção de dna vetor de expressão recombinante célula processo para produzir a enzima que exibe atividade lipolítica enzima que exibe atividade lipolítica preparação de enzima aditivo de detergente e composição de detergente
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
EP0815209B2 (en) 1995-03-17 2015-02-25 Novozymes A/S Novel endoglucanases
ATE429490T1 (de) 1995-05-05 2009-05-15 Novozymes As Protease-varianten und verbindungen
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
AU6414196A (en) 1995-07-14 1997-02-18 Novo Nordisk A/S A modified enzyme with lipolytic activity
WO1997007202A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Novel lipolytic enzymes
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
BR9711479B1 (pt) 1996-09-17 2009-08-11 variante de celulase tendo uma resistência aumentada a tensìdeo de ánion.
CA2265734A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
BR9712473B1 (pt) 1996-11-04 2009-08-11 variantes de subtilase e composições.
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
EP2206786A1 (en) * 1999-08-31 2010-07-14 Novozymes A/S Novel proteases and variants thereof
US6558939B1 (en) * 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
AU2302002A (en) 2000-11-27 2002-06-03 Novozymes As Automated mechanical stress assay for screening cleaning ingredients
ATE399869T1 (de) * 2002-04-10 2008-07-15 Novozymes As Verbesserte bacillus-wirtszelle

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105358686A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
CN105358685A (zh) * 2013-07-29 2016-02-24 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸

Also Published As

Publication number Publication date
US8563289B2 (en) 2013-10-22
EP1713919A2 (en) 2006-10-25
EP2305821A2 (en) 2011-04-06
CN1942584B (zh) 2011-07-27
US20100196990A1 (en) 2010-08-05
JP2007521823A (ja) 2007-08-09
CN1942584A (zh) 2007-04-04
EP2305821A3 (en) 2011-04-13
US20070281332A1 (en) 2007-12-06
WO2005078074A2 (en) 2005-08-25
EP2325318A1 (en) 2011-05-25
WO2005078074A3 (en) 2005-11-03

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Application publication date: 20111123