JP2007504839A - 油性酵母菌における多不飽和脂肪酸の生成 - Google Patents

油性酵母菌における多不飽和脂肪酸の生成 Download PDF

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JP2007504839A
JP2007504839A JP2006532907A JP2006532907A JP2007504839A JP 2007504839 A JP2007504839 A JP 2007504839A JP 2006532907 A JP2006532907 A JP 2006532907A JP 2006532907 A JP2006532907 A JP 2006532907A JP 2007504839 A JP2007504839 A JP 2007504839A
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acid
desaturase
gene encoding
yeast
gene
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JP2007504839A5 (enExample
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ピカタジオ，ステイーブン・ケイ
ヤダブ，ナレンドラ・エス
ズー，シン・サン
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
JP2006532907A 2003-05-07 2004-05-07 油性酵母菌における多不飽和脂肪酸の生成 Pending JP2007504839A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46867703P 2003-05-07 2003-05-07
PCT/US2004/014541 WO2004101757A2 (en) 2003-05-07 2004-05-07 Production of polyunsaturated fatty acids in oleaginous yeasts

Publications (2)

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JP2007504839A true JP2007504839A (ja) 2007-03-08
JP2007504839A5 JP2007504839A5 (enExample) 2007-06-28

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JP2006532907A Pending JP2007504839A (ja) 2003-05-07 2004-05-07 油性酵母菌における多不飽和脂肪酸の生成

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US (5) US7238482B2 (enExample)
EP (4) EP2392664B1 (enExample)
JP (1) JP2007504839A (enExample)
KR (1) KR20060018832A (enExample)
CN (4) CN100591773C (enExample)
BR (1) BRPI0410688A (enExample)
CA (1) CA2526935C (enExample)
DK (3) DK2402448T3 (enExample)
NO (1) NO20055784L (enExample)
WO (1) WO2004101757A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010158219A (ja) * 2009-01-09 2010-07-22 Univ Of Yamanashi 油脂生産菌の培養方法
JP2012501185A (ja) * 2008-08-29 2012-01-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 油性生物中の含油量の変更のためのｓｎｆ１タンパク質キナーゼ活性の操作

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7459546B2 (en) * 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
ATE479757T1 (de) * 2004-06-04 2010-09-15 Fluxome Sciences As Stoffwechseltechnisch hergestellte zellen zur produktion mehrfach ungesättigter fettsäuren
CA2568624C (en) * 2004-06-25 2014-03-25 E. I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US8685679B2 (en) 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
EP1828395A1 (en) * 2004-12-14 2007-09-05 Avestha Gengraine Technologies PVT Ltd Recombinant production docosahexaenoic acid (dha) in yeast
DE102004062141A1 (de) * 2004-12-23 2006-07-06 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls
US7851199B2 (en) * 2005-03-18 2010-12-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
JP5123861B2 (ja) 2005-11-23 2013-01-23 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー Δ９エロンガーゼおよびそれらの多価不飽和脂肪酸製造における使用
US7465793B2 (en) 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
EP2013335A2 (en) 2006-05-01 2009-01-14 E.I. Du Pont De Nemours And Company Fungal delta12 desaturase and delta15 desaturase motifs
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
EP2078092A2 (en) 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
PL2390339T3 (pl) 2006-10-23 2013-12-31 Du Pont Delta-8 desaturazy i ich zastosowanie w wytwarzaniu polinienasyconych kwasów tłuszczowych
CN101617040B (zh) 2006-10-30 2012-07-04 纳幕尔杜邦公司 △17去饱和酶及其在制备多不饱和脂肪酸中的用途
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
EP2441842A1 (en) 2007-02-12 2012-04-18 E. I. du Pont de Nemours and Company Production of arachidonic acid in oilseed plants
UA103595C2 (uk) * 2007-04-03 2013-11-11 Е. І. Дю Пон Де Немур Енд Компані Мультизими і їх застосування в отриманні поліненасичених жирних кислот
US7790156B2 (en) 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US8119860B2 (en) 2007-04-16 2012-02-21 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
EP2152849B1 (en) * 2007-06-01 2013-02-20 Solazyme, Inc. Production of oil in microorganisms
US8673623B2 (en) * 2007-08-31 2014-03-18 Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
CA2699406C (en) 2007-09-12 2019-09-03 Martek Biosciences Corporation Biological oils and production and uses thereof
WO2009046231A1 (en) 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
WO2009126890A2 (en) * 2008-04-10 2009-10-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
WO2010033753A2 (en) 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
BR122021003836B1 (pt) 2008-11-18 2022-02-01 Commonwealth Scientific And Industrial Research Organisation Polinucleotídeo isolado e/ou exógeno que não ocorre de forma natural, vetor e método para produzir óleo contendo ácidos graxos insaturados
WO2010067352A1 (en) * 2008-12-09 2010-06-17 Ben Gurion University Of The Negev Research And Development Authority Polyunsaturated fatty acid elongase
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process
FR2940315B1 (fr) * 2008-12-19 2012-12-14 Airbus France Nouveau procede de culture de levures du genre yarrowia
DE102009007402A1 (de) 2009-02-04 2010-08-05 VLB Berlin e. V. Institut für Mikrobiologie Biosynthese und Gewinnung von Substanzen aus Zellen
US8916154B2 (en) * 2009-04-27 2014-12-23 Abbott Laboratories Antibodies against delta-5 desaturase and uses thereof
EP2475679B1 (en) 2009-06-16 2016-02-10 E. I. du Pont de Nemours and Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
CN102595917A (zh) 2009-06-16 2012-07-18 纳幕尔杜邦公司 来自改善的优化的解脂耶氏酵母菌株的高二十碳五烯酸油
DK2443248T3 (en) 2009-06-16 2018-03-12 Du Pont IMPROVEMENT OF LONG-CHAIN POLYUM Saturated OMEGA-3 AND OMEGA-6 FATTY ACID BIOS SYNTHESIS BY EXPRESSION OF ACYL-CoA LYSOPHOSPHOLIPID ACYL TRANSFERASES
US8637298B2 (en) * 2009-06-16 2014-01-28 E I Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
US20110065161A1 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
WO2011054800A1 (en) * 2009-11-03 2011-05-12 Dsm Ip Assets B.V. Composition comprising cells and a polyunsaturated fatty acid having at least 20 carbon atoms (lc-pufa)
CN104982920A (zh) * 2009-11-03 2015-10-21 帝斯曼知识产权资产管理有限公司 包含细胞和具有至少20个碳原子的多不饱和脂肪酸(lc-pufa)的组合物
US9029108B2 (en) * 2009-11-06 2015-05-12 Diversified Technologies, Inc. Pulsed electric field (PEF) method for continuous enhanced extraction of oil and lipids from small aquatic plants
BR112012015641A2 (pt) 2009-12-24 2015-11-24 Du Pont "polinucleotídeo isolado, construtor de dna recombinante, célula hospedeira, método para produzir uma planta transformada, para aumentar o teor de pelo menos um um ácido graxo, para aumentar pelo menos uma eficiencia de conversão, semente transgênica, plantas, óleos, alimento humano ou de animal e plantas de progênie"
WO2011087981A2 (en) 2010-01-15 2011-07-21 E. I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
JP2013530679A (ja) * 2010-03-31 2013-08-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー トランスジェニック微生物における、目的の非天然生成物の生成を高めるペントースリン酸経路のアップレギュレーション
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
BR112012030333A2 (pt) 2010-05-28 2018-03-13 Solazyme Inc célula microbiana oleaginosa, composição de óleo de triglicerídeo microbiana oleaginosa, e, métodos para produzir uma composição de óleo de triglicerídeo microbiana oleaginosa, e para preparar um produto à base de óleo
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
WO2012021686A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Improved aquaculture meat products
US20120204802A1 (en) 2010-08-11 2012-08-16 E. I. Du Pont De Nemours And Company Sustainable aquaculture feeding strategy
CN103249834B (zh) 2010-08-26 2016-10-26 纳幕尔杜邦公司 生产高水平二十碳五烯酸的重组微生物宿主细胞
AU2011293167B2 (en) 2010-08-26 2016-12-08 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
CN103080317B (zh) 2010-08-26 2017-07-11 纳幕尔杜邦公司 突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途
WO2012061647A2 (en) 2010-11-03 2012-05-10 Solazyme, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
CA2824584A1 (en) 2010-12-30 2012-07-05 E. I. Du Pont De Nemours And Company Use of saccharomyces cerevisiae suc2 gene in yarrowia lipolytica for sucrose utilization
AU2011352082A1 (en) 2010-12-30 2013-06-27 E. I. Du Pont De Nemours And Company Increased oil content by increasing Yap1 transcription factor activity in oleaginous yeasts
JP6071904B2 (ja) 2011-02-02 2017-02-01 テラヴィア ホールディングス， インコーポレイテッド 組み換え油産生微生物から生成される用途に応じた油
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CN103841825B (zh) 2011-02-11 2017-03-22 纳幕尔杜邦公司 从微生物生物质中获取含脂质组合物的方法
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
WO2012135777A1 (en) 2011-04-01 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
CA2833400A1 (en) 2011-05-26 2012-11-29 E.I. Dupont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
DE112011105535B4 (de) * 2011-08-15 2018-08-16 Toyota Jidosha Kabushiki Kaisha Verfahren zum Herstellen eines Alkans und rekombinanter Mikroorganismus, der in der Lage ist, das Alkan zu synthetisieren
US20140322779A1 (en) * 2011-11-11 2014-10-30 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for the producing 1,3-butanediol
WO2013096991A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
KR20150001830A (ko) 2012-04-18 2015-01-06 솔라짐, 인코포레이티드 맞춤 오일
KR102197208B1 (ko) 2012-06-15 2021-01-04 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 식물 세포에서 장쇄 다중불포화 지방산의 생성
CA2877279A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
CN102787128A (zh) * 2012-08-28 2012-11-21 江南大学 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des
BR112015014781A2 (pt) 2012-12-21 2017-10-10 Du Pont célula microbiana recombinante e método de produção de um óleo microbiano
US9005940B2 (en) 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells
KR102271711B1 (ko) 2012-12-24 2021-07-01 퀄리타스 헬스 인코포레이티드 에이코사펜타엔산 (ｅｐａ) 제형
CN105518133A (zh) 2013-07-05 2016-04-20 巴斯夫植物科学有限公司 基因表达或活性增强性元件
WO2015051319A2 (en) 2013-10-04 2015-04-09 Solazyme, Inc. Tailored oils
DE102014220186B4 (de) 2013-12-12 2017-06-22 Technische Universität Dresden Hefestämme und Verfahren zur Produktion von Omega-Hydroxyfettsäuren und Dicarbonsäuren
EA037817B1 (ru) 2013-12-18 2021-05-25 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты
KR20240091350A (ko) * 2014-06-27 2024-06-21 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 도코사펜타에노산을 포함하는 지질
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
US9969990B2 (en) 2014-07-10 2018-05-15 Corbion Biotech, Inc. Ketoacyl ACP synthase genes and uses thereof
EP3325607A1 (en) 2015-07-22 2018-05-30 E. I. du Pont de Nemours and Company High level production of long-chain dicarboxylic acids with microbes
CN105181832A (zh) * 2015-08-31 2015-12-23 广州金域医学检验中心有限公司 一种快速检测全血红细胞二十烷二烯酸的方法
EP3370542A1 (en) 2015-10-01 2018-09-12 DSM IP Assets B.V. Supplement material for use in pet food
AU2019207737B2 (en) 2018-01-10 2023-07-13 Brightseed, Inc. Method for modulating metabolism
WO2020081637A1 (en) * 2018-10-17 2020-04-23 Archer Daniels Midland Company Enzyme addition to fermentation broth for the reduction of oligosaccharides introduced through sterilized dextrose solution
CN110373436B (zh) * 2018-12-11 2022-09-23 山东理工大学 一种产双高-γ-亚麻酸卷枝毛霉细胞工厂的构建方法及发酵技术
CN110229832A (zh) * 2019-06-16 2019-09-13 山东理工大学 一种提高耶式解脂酵母生物量和细胞壁产量的菌株和方法
US11572546B2 (en) 2019-07-29 2023-02-07 Ginkgo Bioworks, Inc. Methods and compositions involving promoters derived from Yarrowia lipolytica
KR102892573B1 (ko) 2019-07-29 2025-12-01 브라이트씨드, 인크. 소화기 건강의 개선 방법
AU2020383340A1 (en) 2019-11-11 2022-06-09 Brightseed, Inc Extract, consumable product and method for enriching bioactive metabolite in an extract
WO2021130078A1 (en) 2019-12-23 2021-07-01 Dsm Ip Assets B.V. Aquaculture feed
WO2022115503A1 (en) 2020-11-25 2022-06-02 Dupont Nutrition Biosciences Aps Treatment and prevention of coronavirus infection
EP4262427A1 (en) 2020-12-17 2023-10-25 DSM IP Assets B.V. Aquaculture feed
AU2022224053A1 (en) * 2021-02-18 2023-09-14 Cemvita Factory, Inc. Systems and methods of making oil from microorganisms
WO2023148154A1 (en) 2022-02-01 2023-08-10 Dsm Ip Assets B.V. Aquaculture feed
CN119464235A (zh) * 2023-08-11 2025-02-18 中国科学院青岛生物能源与过程研究所 Δ12-脂肪酸去饱和酶及应用
WO2025125658A1 (en) 2023-12-14 2025-06-19 Dsm Ip Assets B.V. Aquaculture feed

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001523091A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2001523092A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2002523098A (ja) * 1998-09-02 2002-07-30 アボット・ラボラトリーズ エロンガーゼ遺伝子及びそれらの使用

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ190194A (en) 1978-05-08 1982-02-23 Cpc International Inc Production of oil by fermenting yeast in fatty acids
US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
GB8407195D0 (en) 1984-03-20 1984-04-26 Cadbury Schweppes Plc Microbial desaturase enzyme inhibitors
US4792523A (en) 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
DD227448A1 (de) 1984-10-18 1985-09-18 Akad Wissenschaften Ddr Verfahren zur herstellung von zitronensaeure auf mikrobiellem wege
US4677072A (en) * 1985-02-28 1987-06-30 Westvaco Corporation Rhodotorula having desaturase enzymes
GB8507058D0 (en) 1985-03-19 1985-04-24 Efamol Ltd Pharmaceutical & dietary compositions
GB8524275D0 (en) 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
GB8524276D0 (en) 1985-10-02 1985-11-06 Efamol Ltd Pharmaceutical & dietary compositions
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
DD259637A1 (de) 1987-04-09 1988-08-31 Adw Ddr Verfahren zur herstellung von isozitratlyase in rekombinanten mikroorganismen
SU1454852A1 (ru) 1987-05-19 1989-01-30 Институт Микробиологии Им.Августа Кирхенштейна Штамм дрожжей JaRRoWIa LIроLYтIса-продуцент липазы и питательна среда дл его культивировани
DD267999B5 (de) 1988-01-06 2000-08-10 Akad Wissenschaften Ddr Verfahren zur herstellung von 2-oxoglutarsaeure durch hefen
US5116871A (en) 1988-09-13 1992-05-26 Efamol Holdings Plc Fatty acid therapy and compositions for the treatment of myalgic encephalomyelitis
DD275480A1 (de) 1988-09-15 1990-01-24 Ve Forschungszentrum Biotechno Verfahren zur herstellung von zitronensaeure durch aerobe submerse kultivierung von hefen
US5057419A (en) 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
DD279267A1 (de) 1989-01-05 1990-05-30 Akad Wissenschaften Ddr Verfahren zur herstellung mikrobieller lipase
PL160027B1 (pl) 1989-03-23 1993-01-29 Akad Rolnicza Sposób jednoczesnego otrzymywania kwasu cytrynowego i kwasu izocytrynowego PL
DD285372A5 (de) 1989-06-29 1990-12-12 Ingenieurtechnik,Dd Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen
DD285370A5 (de) 1989-06-29 1990-12-12 Ingenieurtechnik Im Stammbetrieb Veb Kombinat Suesswaren,Dd Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5407957A (en) 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
ATE241007T1 (de) 1990-03-16 2003-06-15 Calgene Llc Dnas, die für pflanzliche desaturasen kodieren und deren anwendungen
US5244921A (en) * 1990-03-21 1993-09-14 Martek Corporation Eicosapentaenoic acids and methods for their production
CA2083259C (en) 1990-05-25 2007-05-15 William D. Hitz Nucleotide sequence of soybean stearoyl-acp desaturase gene
US5658767A (en) 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
JPH07501701A (ja) 1991-12-04 1995-02-23 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 植物由来の脂肪酸不飽和化酵素遺伝子
DE69321885T2 (de) 1992-06-25 1999-05-12 International Flavors & Fragrances Inc., New York, N.Y. Verfahren zur fermentativen Herstellung von 10-Hydroxy-C18-Carbonsäure und Gamma-Dodekalaktonderivaten
IT1256043B (it) 1992-08-12 1995-11-21 Ceppo mutante di yarrowia lipolytica e procedimento per la produzione di acido citrico
ES2198408T3 (es) 1992-11-17 2004-02-01 E.I. Du Pont De Nemours And Company Genes para delta 12 desaturasas de acidos grasos microsomales y enzimas afines de plantas.
FR2734843A1 (fr) 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0770683A1 (en) 1995-10-04 1997-05-02 Mitsubishi Chemical Corporation Method for producing erythritol
JPH09252790A (ja) 1996-03-21 1997-09-30 Japan Energy Corp 発酵法によるピルビン酸の製造方法
DE69711374T3 (de) * 1996-05-15 2007-05-16 Dsm Ip Assets B.V. Verfahren zum extrahieren von sterol mit einem polaren lösungsmittel zur herstellung eines mikrobiellen öles mit niedrigem sterolgehalt
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
RU2090611C1 (ru) 1996-09-27 1997-09-20 Финогенова Татьяна Васильевна Штамм дрожжей yarrowia lipolytica - продуцент лимонной кислоты, способ получения лимонной кислоты и способ выделения цитрата натрия
US6432684B1 (en) * 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US6075183A (en) 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
JP2002503447A (ja) 1997-12-04 2002-02-05 スミスクライン・ビーチャム・コーポレイション 発現可能なアンチセンス配列を用いる条件発現変異細胞の取得方法
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
EP0997533A1 (de) 1998-10-24 2000-05-03 Haarmann & Reimer Gmbh Verfahren zur Gewinnung von Gamma-Decalacton
DK1276874T3 (da) 2000-04-28 2008-02-04 Mayoly Spindler Lab Kloning og ekspression af en syreresistent ekstracellulær lipase fra yarrowia lipolytica
US7083972B2 (en) 2000-05-19 2006-08-01 Kaneka Corporation Transformant and process for producing polyester by using the same
WO2002081668A2 (en) 2001-01-25 2002-10-17 Abbott Laboratories Desaturase genes and uses thereof
JP2002296554A (ja) 2001-03-30 2002-10-09 Minebea Co Ltd ファラデー回転子
US7045683B2 (en) 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
US7211656B2 (en) 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
AU2003245308B2 (en) 2002-05-22 2008-02-07 Monsanto Technology Llc Fatty acid desaturases from fungi
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7364883B2 (en) 2004-05-07 2008-04-29 Yeastern Biotech Co., Ltd. Process for producing poly-unsaturated fatty acids by oleaginous yeasts

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001523091A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2001523092A (ja) * 1997-04-11 2001-11-20 カルジーン エル エル シー 長鎖多不飽和脂肪酸の合成のための方法および組成物
JP2002523098A (ja) * 1998-09-02 2002-07-30 アボット・ラボラトリーズ エロンガーゼ遺伝子及びそれらの使用

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
JPN6010011404; Ｂｉｏｃｈｅｍｉｃａｌ　ａｎｄ　Ｂｉｏｐｈｙｓｉｃａｌ　Ｒｅｓｅａｒｃｈ　Ｃｏｍｍｕｎｉｃａｔｉｏｎｓ ｖｏｌ．２６３, 1999, ｐｐ．４７-５１ *
JPN6010011406; Ｅｕｒ．　Ｊ．　Ｂｉｏｃｈｅｍ ｖｏｌ．２６１, 1999, ｐｐ．８１２-８２０ *
JPN6010011409; Ｂｉｏｃｈｅｍｉｃａｌ　Ｓｏｃｉｅｔｙ　Ｔｒａｎｓａｃｔｉｏｎｓ ｖｏｌ．２８　ｐａｒｔ６, 2000, ｐｐ．６３２-６３５ *
JPN6011034965; Ｂｉｏｒｅｓｏｕｒｃｅ　Ｔｅｃｈｎｏｌｏｇｙ ｖｏｌ．８２, 2002, ｐｐ．４３-４９ *
JPN6011034967; Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｃｅｌｌ　Ｂｉｏｌｏｇｙ ｖｏｌ．１３７　ｎｏ．６, 1997, ｐｐ．１２６５-１２７８ *
JPN6011034970; ＹＥＡＳＴ ｖｏｌ．１４, 1998, ｐｐ．１２６７-１２８３ *
JPN6011034974; ＦＥＭＳ　Ｍｉｃｒｏｂｉｏｌｏｇｙ　Ｒｅｖｉｅｗｓ ｖｏｌ．１９, 1997, ｐｐ．２１９-２３７ *
JPN6014019854; Ｎｕｃｌｅｉｃ　Ａｃｉｄｓ　Ｒｅｓｅａｒｃｈ，１９８７年，ｖｏｌ．１５，Ｎｏ．８，ｐｐ．３５８１-３５９３ , 1987

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012501185A (ja) * 2008-08-29 2012-01-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 油性生物中の含油量の変更のためのｓｎｆ１タンパク質キナーゼ活性の操作
JP2010158219A (ja) * 2009-01-09 2010-07-22 Univ Of Yamanashi 油脂生産菌の培養方法

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