DK2392664T3 - Preparation of polyunsaturated fatty acids in OIL YEAST - Google Patents
Preparation of polyunsaturated fatty acids in OIL YEAST Download PDFInfo
- Publication number
- DK2392664T3 DK2392664T3 DK11154995.2T DK11154995T DK2392664T3 DK 2392664 T3 DK2392664 T3 DK 2392664T3 DK 11154995 T DK11154995 T DK 11154995T DK 2392664 T3 DK2392664 T3 DK 2392664T3
- Authority
- DK
- Denmark
- Prior art keywords
- desaturase
- ala
- leu
- val
- gly
- Prior art date
Links
- TWSHAOXXFOESKM-UHFFFAOYSA-N C(C1)C1C1NC1 Chemical compound C(C1)C1C1NC1 TWSHAOXXFOESKM-UHFFFAOYSA-N 0.000 description 1
- VVVQCJNBGKIIMU-UHFFFAOYSA-N C(C1C=C1)C1N=CC1 Chemical compound C(C1C=C1)C1N=CC1 VVVQCJNBGKIIMU-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US46867703P | 2003-05-07 | 2003-05-07 | |
| EP04751769.3A EP1620543B1 (en) | 2003-05-07 | 2004-05-07 | Production of polyunsaturated fatty acids in oleaginous yeasts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2392664T3 true DK2392664T3 (en) | 2016-05-09 |
Family
ID=33452224
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11154995.2T DK2392664T3 (en) | 2003-05-07 | 2004-05-07 | Preparation of polyunsaturated fatty acids in OIL YEAST |
| DK11155007.5T DK2402448T3 (en) | 2003-05-07 | 2004-05-07 | Preparation of polyunsaturated fatty acids in OIL YEAST |
| DK04751769.3T DK1620543T3 (en) | 2003-05-07 | 2004-05-07 | PREPARATION OF POLYUM Saturated FAT ACIDS IN OIL Yeast |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11155007.5T DK2402448T3 (en) | 2003-05-07 | 2004-05-07 | Preparation of polyunsaturated fatty acids in OIL YEAST |
| DK04751769.3T DK1620543T3 (en) | 2003-05-07 | 2004-05-07 | PREPARATION OF POLYUM Saturated FAT ACIDS IN OIL Yeast |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US7238482B2 (enExample) |
| EP (4) | EP2392664B1 (enExample) |
| JP (1) | JP2007504839A (enExample) |
| KR (1) | KR20060018832A (enExample) |
| CN (4) | CN1816630A (enExample) |
| BR (1) | BRPI0410688A (enExample) |
| CA (1) | CA2526935C (enExample) |
| DK (3) | DK2392664T3 (enExample) |
| NO (1) | NO20055784L (enExample) |
| WO (1) | WO2004101757A2 (enExample) |
Families Citing this family (113)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110059496A1 (en) * | 2003-06-25 | 2011-03-10 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| US7459546B2 (en) * | 2003-06-25 | 2008-12-02 | E.I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast |
| US7267976B2 (en) * | 2003-07-02 | 2007-09-11 | E.I. Du Pont De Nemours And Company | Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts |
| BRPI0511236A (pt) * | 2004-06-04 | 2007-11-27 | Fluxome Sciences As | método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado |
| BRPI0512481A (pt) * | 2004-06-25 | 2008-03-11 | Du Pont | polinucleotìdeo isolado, polipeptìdeo, construção recombinante, célula, yarrowia sp.transformada, métodos para transformar uma célula, produzir uma planta transformada, produzir levedura, produzir ácidos graxos poliinsaturados e produzir pelo menos um ácido graxo poliinsaturados, sementes, óleos, plantas de sementes oleaginosa e alimentos ou rações |
| US7465564B2 (en) * | 2004-08-10 | 2008-12-16 | E.I. Du Pont De Nemours And Company | Production of γ-linolenic acid in oleaginous yeast |
| US7264949B2 (en) | 2004-09-15 | 2007-09-04 | E.I. Du Pont De Nemours And Company | Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast |
| US7550286B2 (en) * | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| US7273746B2 (en) | 2004-11-04 | 2007-09-25 | E.I. Dupont De Nemours And Company | Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| US7192762B2 (en) | 2004-11-04 | 2007-03-20 | E. I. Du Pont De Nemours And Company | Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| US20060094102A1 (en) | 2004-11-04 | 2006-05-04 | Zhixiong Xue | Ammonium transporter promoter for gene expression in oleaginous yeast |
| US8685679B2 (en) | 2004-11-04 | 2014-04-01 | E I Du Pont De Nemours And Company | Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms |
| US7189559B2 (en) * | 2004-11-04 | 2007-03-13 | E. I. Du Pont De Nemours And Company | Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| EP1828395A1 (en) * | 2004-12-14 | 2007-09-05 | Avestha Gengraine Technologies PVT Ltd | Recombinant production docosahexaenoic acid (dha) in yeast |
| DE102004062141A1 (de) * | 2004-12-23 | 2006-07-06 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls |
| BRPI0609040B1 (pt) * | 2005-03-18 | 2018-07-31 | Microbia, Inc. | Fungo recombinante do gênero yarrowia, método de produção de um carotenóide e método de preparo de um aditivo alimentício ou ração contendo um carotenóide |
| US7470532B2 (en) * | 2005-10-19 | 2008-12-30 | E.I. Du Pont De Nemours And Company | Mortierella alpina C16/18 fatty acid elongase |
| DK1951866T3 (da) | 2005-11-23 | 2014-10-27 | Du Pont | Delta-9-elongaser og anvendelse heraf til fremstilling af flerumættede fedtsyrer |
| US7465793B2 (en) | 2006-04-20 | 2008-12-16 | E.I. Du Pont De Nemours And Company | Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids |
| US7943823B2 (en) | 2006-04-28 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| US20070254299A1 (en) | 2006-05-01 | 2007-11-01 | Yadav Narendra S | Fungal delta 12 desaturase and delta 15 desaturase motifs |
| US7695950B2 (en) * | 2006-05-17 | 2010-04-13 | E. I. Du Pont De Nemours And Company | Δ5 desaturase and its use in making polyunsaturated fatty acids |
| US7678560B2 (en) * | 2006-05-17 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Δ 5 desaturase and its use in making polyunsaturated fatty acids |
| EP2078092A2 (en) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| US20110016581A1 (en) | 2006-10-23 | 2011-01-20 | E.I. Du Pont De Nemours And Company | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
| US7556949B2 (en) | 2006-10-30 | 2009-07-07 | E.I. Du Pont De Nemours And Company | Δ17 desaturase and its use in making polyunsaturated fatty acids |
| US7709239B2 (en) * | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
| US8846374B2 (en) | 2006-12-12 | 2014-09-30 | E I Du Pont De Nemours And Company | Carotenoid production in a recombinant oleaginous yeast |
| WO2008100545A2 (en) | 2007-02-12 | 2008-08-21 | E. I. Du Pont De Nemours And Company | Production of arachidonic acid in oilseed plants |
| ES2559312T3 (es) * | 2007-04-03 | 2016-02-11 | E. I. Du Pont De Nemours And Company | Multienzimas y su uso en la fabricación de ácidos grasos poliinsaturados |
| US7790156B2 (en) * | 2007-04-10 | 2010-09-07 | E. I. Du Pont De Nemours And Company | Δ-8 desaturases and their use in making polyunsaturated fatty acids |
| US7794701B2 (en) | 2007-04-16 | 2010-09-14 | E.I. Du Pont De Nemours And Company | Δ-9 elongases and their use in making polyunsaturated fatty acids |
| US7943365B2 (en) | 2007-05-03 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Δ-5 desaturases and their use in making polyunsaturated fatty acids |
| CN104212844A (zh) * | 2007-06-01 | 2014-12-17 | 索拉兹米公司 | 在微生物中生产油 |
| US8673623B2 (en) * | 2007-08-31 | 2014-03-18 | Board Of Regents, The University Of Texas System | Apparatus for performing magnetic electroporation |
| MX2010002825A (es) | 2007-09-12 | 2010-08-31 | Martek Biosciences Corp | Aceites biologicos y produccion y usos de los mismos. |
| JP5711968B2 (ja) | 2007-10-03 | 2015-05-07 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company | 高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ(YarrowiaLipolytica)の最適化株 |
| WO2009126890A2 (en) * | 2008-04-10 | 2009-10-15 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| US8168858B2 (en) | 2008-06-20 | 2012-05-01 | E. I. Du Pont De Nemours And Company | Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids |
| CN102216464B (zh) * | 2008-08-29 | 2014-11-26 | 纳幕尔杜邦公司 | 操纵snf1蛋白激酶活性用于改变含油生物中的油含量 |
| CN102197047B (zh) | 2008-09-19 | 2014-07-09 | 纳幕尔杜邦公司 | 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 |
| CN113957054B (zh) | 2008-11-18 | 2024-07-16 | 联邦科学技术研究组织 | 产生ω-3脂肪酸的酶和方法 |
| US8802924B2 (en) * | 2008-12-09 | 2014-08-12 | Ben Gurion University Of The Negev Research And Development Authority | Polyunsaturated fatty acid elongase |
| CA2744716C (en) | 2008-12-18 | 2017-05-23 | E.I. Du Pont De Nemours And Company | Reducing byproduction of malonates in a fermentation process |
| FR2940315B1 (fr) * | 2008-12-19 | 2012-12-14 | Airbus France | Nouveau procede de culture de levures du genre yarrowia |
| JP5709196B2 (ja) * | 2009-01-09 | 2015-04-30 | 国立大学法人山梨大学 | 油脂生産菌の培養方法 |
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| US8524485B2 (en) * | 2009-06-16 | 2013-09-03 | E I Du Pont De Nemours And Company | Long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-CoA lysophospholipid acyltransferases |
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| CA2763424C (en) | 2009-06-16 | 2018-06-12 | E.I. Du Pont De Nemours And Company | Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases |
| DK2442666T3 (da) | 2009-06-16 | 2015-07-13 | Du Pont | Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica |
| US20110065161A1 (en) * | 2009-09-14 | 2011-03-17 | Board Of Regents, The University Of Texas System | Bipolar solid state marx generator |
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| CN106135460B (zh) | 2010-05-28 | 2020-07-24 | 柯碧恩生物技术公司 | 包含特制油的食品组合物 |
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| WO2012027676A1 (en) | 2010-08-26 | 2012-03-01 | E. I. Du Pont De Nemours And Company | Mutant delta-9 elongases and their use in making polyunsaturated fatty acids |
| CA2816125C (en) | 2010-11-03 | 2018-12-11 | Solazyme, Inc. | Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods |
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| AU2012327162A1 (en) | 2011-12-27 | 2013-07-11 | Commonwealth Scientific And Industrial Research Organisation | Production of dihydrosterculic acid and derivatives thereof |
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- 2004-05-07 CN CNA200480019355XA patent/CN1816630A/zh active Pending
- 2004-05-07 EP EP11154995.2A patent/EP2392664B1/en not_active Expired - Lifetime
- 2004-05-07 BR BRPI0410688 patent/BRPI0410688A/pt not_active Application Discontinuation
- 2004-05-07 DK DK11154995.2T patent/DK2392664T3/en active
- 2004-05-07 CN CN200910266075A patent/CN101824441A/zh active Pending
- 2004-05-07 EP EP20110155002 patent/EP2392665A3/en not_active Withdrawn
- 2004-05-07 WO PCT/US2004/014541 patent/WO2004101757A2/en not_active Ceased
- 2004-05-07 DK DK11155007.5T patent/DK2402448T3/en active
- 2004-05-07 CN CN200480019580A patent/CN100591773C/zh not_active Expired - Fee Related
- 2004-05-07 KR KR20057021008A patent/KR20060018832A/ko not_active Withdrawn
- 2004-05-07 DK DK04751769.3T patent/DK1620543T3/en active
- 2004-05-07 CN CNA2004800193545A patent/CN1816559A/zh active Pending
- 2004-05-07 JP JP2006532907A patent/JP2007504839A/ja active Pending
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