JP2005512577A5 - - Google Patents

Download PDF

Info

Publication number
JP2005512577A5
JP2005512577A5 JP2003554933A JP2003554933A JP2005512577A5 JP 2005512577 A5 JP2005512577 A5 JP 2005512577A5 JP 2003554933 A JP2003554933 A JP 2003554933A JP 2003554933 A JP2003554933 A JP 2003554933A JP 2005512577 A5 JP2005512577 A5 JP 2005512577A5
Authority
JP
Japan
Prior art keywords
primer
melting temperature
concentration
probe
adjusted melting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2003554933A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005512577A (ja
JP4355211B2 (ja
Filing date
Publication date
Priority claimed from US10/320,893 external-priority patent/US7198897B2/en
Application filed filed Critical
Publication of JP2005512577A publication Critical patent/JP2005512577A/ja
Publication of JP2005512577A5 publication Critical patent/JP2005512577A5/ja
Application granted granted Critical
Publication of JP4355211B2 publication Critical patent/JP4355211B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

JP2003554933A 2001-12-19 2002-12-19 Late−pcr Expired - Fee Related JP4355211B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34188601P 2001-12-19 2001-12-19
US10/320,893 US7198897B2 (en) 2001-12-19 2002-12-17 Late-PCR
PCT/US2002/040752 WO2003054233A1 (en) 2001-12-19 2002-12-19 Late-pcr

Publications (3)

Publication Number Publication Date
JP2005512577A JP2005512577A (ja) 2005-05-12
JP2005512577A5 true JP2005512577A5 (enExample) 2006-01-26
JP4355211B2 JP4355211B2 (ja) 2009-10-28

Family

ID=26982708

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2003554933A Expired - Fee Related JP4355211B2 (ja) 2001-12-19 2002-12-19 Late−pcr

Country Status (11)

Country Link
US (3) US7198897B2 (enExample)
EP (1) EP1468114B1 (enExample)
JP (1) JP4355211B2 (enExample)
AT (1) ATE394508T1 (enExample)
AU (1) AU2002360685A1 (enExample)
CA (1) CA2511128C (enExample)
DE (1) DE60226500D1 (enExample)
DK (1) DK1468114T3 (enExample)
ES (1) ES2306807T3 (enExample)
PT (1) PT1468114E (enExample)
WO (1) WO2003054233A1 (enExample)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030108919A1 (en) * 2001-09-05 2003-06-12 Perlegen Sciences, Inc. Methods for amplification of nucleic acids
AU2004209426B2 (en) * 2003-01-29 2008-04-17 454 Life Sciences Corporation Method for preparing single-stranded DNA libraries
EP1454994A1 (en) * 2003-03-07 2004-09-08 Université de la Méditerranée Standardized and optimized real-time quantitative reverse transcriptase polymerase chain reaction method for detection of MRD in leukemia
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
AU2004235747B2 (en) 2003-05-01 2009-05-28 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
DE602004030785D1 (de) * 2003-05-19 2011-02-10 Univ Brandeis Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
CA2553833C (en) * 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
JP4455168B2 (ja) * 2004-05-31 2010-04-21 株式会社ニチレイフーズ Dna増幅方法
WO2006085964A2 (en) 2004-06-30 2006-08-17 Applera Corporation Log-linear amplification
JP2006055065A (ja) * 2004-08-19 2006-03-02 Toyo Kohan Co Ltd 非対称pcrを用いた核酸の検出方法、ポリヌクレオチドプローブ、それを固定するプローブ固定化担体およびキット
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
ES2529204T3 (es) * 2004-10-18 2015-02-17 Brandeis University Métodos para la amplificación de ácido nucleico
CN101076608B (zh) 2004-10-18 2013-04-17 布兰迪斯大学 在pcr扩增中提高再现性和降低引物错导的试剂和方法
US20080113354A1 (en) * 2004-11-12 2008-05-15 Yanggu Shi Methods and Compositions for High Sensitivity Fluorescent Mutation Detection with Mismatch Cutting Dna Endonucleases
AU2006272568B2 (en) 2005-07-26 2012-06-28 Merck Sharp & Dohme Corp. Assays for resistance to echinocandin-class drugs
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
WO2007141700A2 (en) * 2006-06-08 2007-12-13 Koninklijke Philips Electronics N. V. Microelectronic sensor device for dna detection
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
WO2008011004A2 (en) 2006-07-17 2008-01-24 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
US7501254B2 (en) 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
JP2008154467A (ja) * 2006-12-21 2008-07-10 Olympus Corp 核酸の増幅方法とこれを用いた核酸の解析方法
EP2574681B1 (en) 2007-03-28 2016-03-23 Signal Diagnostics System and method for high resolution analysis of nucleic acids to detect sequence variations
JP2009148245A (ja) * 2007-06-12 2009-07-09 Toyobo Co Ltd 核酸の迅速な検出方法
GB0720264D0 (en) * 2007-10-17 2007-11-28 Smiths Detection Watford Ltd Sample preparation devices and analyzers
WO2009062166A2 (en) * 2007-11-08 2009-05-14 University Of Washington Dna microarray based identification and mapping of balanced translocation breakpoints
EP2138588A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
BRPI0915886A2 (pt) 2008-06-30 2015-11-03 Life Technologies Corp método para amplificação direta a partir de amostras brutas de ácido nucléico
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
CA3075139C (en) 2008-09-23 2022-04-12 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9598725B2 (en) * 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) * 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
WO2016007914A1 (en) * 2014-07-10 2016-01-14 Fluoresentric, Inc. Dna amplification technology
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
CA2755207A1 (en) 2009-03-12 2010-09-16 Brandeis University Reagents and methods for pcr
EP2456887B1 (en) * 2009-07-21 2015-11-25 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
JP6155418B2 (ja) 2009-09-02 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 多重エマルジョンの合体による、流体を混合するためのシステム
EP2980224B1 (en) 2009-10-21 2019-07-03 Brandeis University Kits for analyzing single-stranded nucleic acid sequences
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc TRAPPING TRANSPORT AND DETECTION SYSTEM
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
AU2011255641A1 (en) 2010-05-18 2012-12-06 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
EP2606151A2 (en) 2010-08-17 2013-06-26 Smiths Detection-Watford Limited Ndm-1 polymerase chain reaction (pcr) assay
EP2614157A4 (en) * 2010-09-08 2014-03-26 Univ Brandeis Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
WO2012048207A2 (en) * 2010-10-07 2012-04-12 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
EP3574990B1 (en) 2010-11-01 2022-04-06 Bio-Rad Laboratories, Inc. System for forming emulsions
US10273525B2 (en) 2010-11-10 2019-04-30 Brandeis University Compositions, methods, and kits for detecting and identifying mycobacteria
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
EP3181704B1 (en) * 2010-12-27 2021-01-27 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ, インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2013044097A1 (en) 2011-09-21 2013-03-28 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
US9121051B2 (en) 2011-10-31 2015-09-01 Arkray, Inc. Method of determining the abundance of a target nucleotide sequence of a gene of interest
CN104024433B (zh) 2011-10-31 2016-11-16 爱科来株式会社 基因丰度的测定方法
WO2013122453A1 (en) * 2012-02-15 2013-08-22 Universiti Sains Malaysia Device and method for detection of nucleic acid(s)
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
JP2015536653A (ja) * 2012-11-15 2015-12-24 モレキュラー ディテクション イスラエル リミテッド Pcr反応混合物及びそれを使用する方法
SG11201506146TA (en) 2013-02-07 2015-09-29 Univ Rutgers Highly selective nucleic acid amplification primers
WO2014160046A1 (en) * 2013-03-14 2014-10-02 The Trustees Of The University Of Pennsylvania A method for detecting mutations in single cells or single molecules
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
CN105793435A (zh) 2013-10-09 2016-07-20 弗洛雷森特里克公司 多重探针
PL3089822T3 (pl) * 2013-12-30 2022-09-19 Atreca, Inc. Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych
JP6659575B2 (ja) 2014-04-21 2020-03-04 ナテラ, インコーポレイテッド 変異の検出および染色体分節の倍数性
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
WO2016014921A1 (en) * 2014-07-24 2016-01-28 Brandies University Linear-expo-linear pcr (lel-pcr)
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
RS60032B1 (sr) * 2015-05-18 2020-04-30 Saga Diagnostics Ab Detekcija ciljne nukleinske kiseline i varijanti
EP3859333A1 (en) * 2015-09-10 2021-08-04 InSilixa, Inc. Systems for multiplex quantitative nucleic acid amplification
US9499861B1 (en) * 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017087943A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
AU2017246663B2 (en) 2016-04-07 2022-09-01 Rutgers, The State University Of New Jersey Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor
CN109477138A (zh) 2016-04-15 2019-03-15 纳特拉公司 肺癌检测方法
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10426424B2 (en) 2017-11-21 2019-10-01 General Electric Company System and method for generating and performing imaging protocol simulations
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CN112236535A (zh) 2018-04-14 2021-01-15 纳特拉公司 用于借助于循环肿瘤dna的个人化检测的癌症检测和监测的方法
CN112534064A (zh) * 2018-06-07 2021-03-19 拜奥法尔诊断有限责任公司 快速热循环方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN113924041B (zh) 2019-03-14 2024-12-03 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
IL291819A (en) 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
CN112335926B (zh) 2020-11-30 2025-03-11 厦门烟草工业有限责任公司 烟草加香机

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5627054A (en) * 1996-04-05 1997-05-06 The United States Of America As Represented By The Secretary Of The Army Competitor primer asymmetric polymerase chain reaction
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US6333179B1 (en) 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products
US6277607B1 (en) * 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
CA2403708A1 (en) * 2000-03-22 2001-09-27 Quantum Dot Corporation Methods of using semiconductor nanocrystals in bead-based nucleic acid assays
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR

Similar Documents

Publication Publication Date Title
JP2005512577A5 (enExample)
AU2008272421B2 (en) Chimeric primers for improved nucleic acid amplification reactions
CA2511128A1 (en) Late-pcr
CA2790153C (en) Thd primer target detection
KR101380414B1 (ko) 다중 호흡기 바이러스의 동시 검출 방법, 및 이의 용도
CN109642251B (zh) 核酸扩增过程中的改进或与核酸扩增过程相关的改进
JP2003510020A5 (enExample)
KR102295290B1 (ko) Dna 증폭 기술
JP2013507971A (ja) 反応特異性の増加のための非標準塩基を含む増幅プライマー
US20200032310A1 (en) Mispriming prevention reagents
US20210087607A1 (en) Methods and compositions for nucleic acid detection
EP2373808A1 (en) Enhanced taqman probe based amplification
JP2021509814A5 (enExample)
WO2007067151A1 (en) Fluorescent primer system for detection of nucleic acids (q priming)
KR101378920B1 (ko) Braf 돌연변이의 선택적 검출 방법 및 이를 이용한 키트
JP2018183153A (ja) メルティングピーク分析を利用したターゲット核酸配列の定量
AU2009241289A1 (en) Isothermal detection methods and uses thereof
Pestana et al. Real-time PCR–the basic principles
WO2023073143A1 (en) Nucleic acid amplification method
KR20240058023A (ko) 대장균의 O104, O124, O55, O157, O128ab, O76, O185, O130, O81, O11 또는 O153 혈청형 신속 동정용 프라이머 세트 및 이를 이용한 대장균의 혈청형 동정 방법
KR20240058021A (ko) 대장균의 o103, o121, o123, o136, o156, o168, o170, o174, o3, o40, o64, o66, o6, o78, o80, o82, o88, o8, o91, o181 또는 o111 혈청형 신속 동정용 프라이머 세트 및 이를 이용한 대장균의 혈청형 동정 방법
JP2003000251A5 (enExample)
Fantozzi et al. Screening for mRNA Expression Using the Polymerase Chain Reaction
WO2020027096A1 (ja) プライマー及びその使用
JP2010088355A (ja) プライマー試薬およびそれを用いた核酸増幅方法