DE602004030785D1 - Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren - Google Patents

Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren

Info

Publication number
DE602004030785D1
DE602004030785D1 DE602004030785T DE602004030785T DE602004030785D1 DE 602004030785 D1 DE602004030785 D1 DE 602004030785D1 DE 602004030785 T DE602004030785 T DE 602004030785T DE 602004030785 T DE602004030785 T DE 602004030785T DE 602004030785 D1 DE602004030785 D1 DE 602004030785D1
Authority
DE
Germany
Prior art keywords
kits
nucleic acids
devices
rna
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004030785T
Other languages
English (en)
Inventor
Lawrence J Wangh
Cristina Hartshorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Publication of DE602004030785D1 publication Critical patent/DE602004030785D1/de
Anticipated expiration legal-status Critical
Active legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
DE602004030785T 2003-05-19 2004-05-18 Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren Active DE602004030785D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47189503P 2003-05-19 2003-05-19
PCT/US2004/015611 WO2004104181A2 (en) 2003-05-19 2004-05-18 Nucleic acid processing methods, kits and devices

Publications (1)

Publication Number Publication Date
DE602004030785D1 true DE602004030785D1 (de) 2011-02-10

Family

ID=33476896

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004030785T Active DE602004030785D1 (de) 2003-05-19 2004-05-18 Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren

Country Status (8)

Country Link
US (2) US7465562B2 (de)
EP (1) EP1631685B1 (de)
JP (1) JP4836795B2 (de)
AT (1) ATE493508T1 (de)
CA (1) CA2526409A1 (de)
DE (1) DE602004030785D1 (de)
ES (1) ES2355899T3 (de)
WO (1) WO2004104181A2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008526204A (ja) * 2004-12-30 2008-07-24 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション φ１５ＤＮＡポリメラーゼ
JP5073967B2 (ja) 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
WO2007140417A2 (en) * 2006-05-31 2007-12-06 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US20080003574A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Kits for RNA extraction
EP2147120B1 (de) * 2007-05-03 2014-04-02 Roche Diagnostics GmbH VERBESSERTE LYSIS UND REVERSE TRANSKRIPTION ZUR mRNA-QUANTIFIZIERUNG
JP4556194B2 (ja) 2008-02-01 2010-10-06 セイコーエプソン株式会社 生体試料反応方法
CN102112631A (zh) 2008-08-01 2011-06-29 霍夫曼-拉罗奇有限公司 用于mRNA扩增的改进的裂解和逆转录
CN102292635B (zh) * 2008-09-22 2015-04-01 霍夫曼-拉罗奇有限公司 微阵列底物上的生物材料的选择性处理
WO2010088517A1 (en) * 2009-01-30 2010-08-05 The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Methods and systems for purifying transferring and/or manipulating nucleic acids
JP5766178B2 (ja) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ ＳｌｉｐＣｈｉｐ装置および方法
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
GB0919159D0 (en) * 2009-11-02 2009-12-16 Sec Dep For Environment Food A Device and apparatus
ES2543367T3 (es) * 2010-07-07 2015-08-18 Diagon Kft. Procedimiento para el aislamiento específico del contenido de ADN total de gérmenes bacterianos y un kit para dicho fin
DE102011078342A1 (de) * 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit
JP6301260B2 (ja) 2012-10-26 2018-03-28 株式会社カネカ Ｒｎａ調製法
US10253353B2 (en) 2013-12-06 2019-04-09 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
JP2017209036A (ja) * 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法
SI3568475T1 (sl) 2017-01-16 2023-05-31 Spectrum Solutions L. L. C. Raztopina za ohranjanje nukleinske kisline in načini uporabe
CN106867993A (zh) * 2017-02-17 2017-06-20 唐旌生物科技（上海）有限公司 一种逆转录方法、逆转录试剂盒及其应用
EA202191395A1 (ru) 2018-11-20 2021-08-23 СПЕКТРУМ СОЛЮШНЗ, ЭлЭлСи Система сбора образцов, содержащая уплотнительную крышку и клапан
CN112094840A (zh) * 2019-06-17 2020-12-18 南京尧顺禹生物科技有限公司 一种用于基因组片段扩增的微量动物样品基因组dna模板的快速制备方法
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4585623A (en) * 1984-02-27 1986-04-29 Allelix Inc. Device for performing quantitative chemical and immunochemical assays
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
WO1993025710A1 (en) * 1992-06-08 1993-12-23 Gen-Probe Incorporated Preparation of nucleic acid from mononuclear cells
JPH0646900A (ja) * 1992-07-29 1994-02-22 Tonen Corp 単一容器内での逆転写酵素介在二段階遺伝子増幅反応によるｒｎａ遺伝子の増幅検出方法
US5407810A (en) * 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
ATE347615T1 (de) 1997-04-16 2006-12-15 Applera Corp Nukleinsäuresammlung
US6872527B2 (en) * 1997-04-16 2005-03-29 Xtrana, Inc. Nucleic acid archiving
US6440725B1 (en) * 1997-12-24 2002-08-27 Cepheid Integrated fluid manipulation cartridge
US6111096A (en) 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
CA2310217A1 (en) * 1997-11-26 1999-06-03 Inhibitex, Inc. Extracellular matrix-binding proteins from staphylococcus aureus
US6087182A (en) * 1998-08-27 2000-07-11 Abbott Laboratories Reagentless analysis of biological samples
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
AU764340B2 (en) * 1998-11-06 2003-08-14 Universite De Montreal Improved bactericidal and non-bactericidal solutions for removing biofilms
US6927024B2 (en) * 1998-11-30 2005-08-09 Genentech, Inc. PCR assay
US6458259B1 (en) * 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
WO2002020842A1 (en) * 2000-09-06 2002-03-14 Transnetyx, Inc. System, method and apparatus for transgenic and targeted mutagenesis screening
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
ES2604352T3 (es) * 2003-02-05 2017-03-06 Iquum, Inc. Procesado de muestras
KR20060036901A (ko) * 2003-05-02 2006-05-02 시그마-알드리치컴퍼니 고상 세포 용해 및 포획 플랫폼

Also Published As

Publication number Publication date
JP2006528002A (ja) 2006-12-14
ES2355899T3 (es) 2011-04-01
CA2526409A1 (en) 2004-12-02
US20090111170A1 (en) 2009-04-30
US9279121B2 (en) 2016-03-08
WO2004104181A3 (en) 2005-12-22
US20060275773A1 (en) 2006-12-07
ATE493508T1 (de) 2011-01-15
EP1631685A4 (de) 2007-08-29
EP1631685B1 (de) 2010-12-29
EP1631685A2 (de) 2006-03-08
JP4836795B2 (ja) 2011-12-14
WO2004104181A2 (en) 2004-12-02
US7465562B2 (en) 2008-12-16

Similar Documents

Publication Publication Date Title
ATE493508T1 (de) Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
KR102088381B1 (ko) 압타머-기반 다중 검정법
CN101200716B (zh) 使用聚多卡醇和衍生物的核酸分离
Ma et al. Microfluidics for genome-wide studies involving next generation sequencing
ATE314488T1 (de) Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben
US20080176320A1 (en) Selective Lysis of Sperm Cells
CA2354379A1 (en) Method for isolating dna from biological materials
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
ATE549414T1 (de) Verfahren zur isolierung von zellen
KR20030045106A (ko) 비-병원성 또는 병원성 ｈ5 아형 인플루엔자 ａ 바이러스검출용 키트
BRPI0610092B8 (pt) métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado
WO2001057247A3 (de) Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind
US20180010168A1 (en) One-step procedure for the purification of nucleic acids
CN107406892B (zh) 捕获精子核酸的方法
EP1932913A1 (de) Nukleinsäureisolation mithilfe von Polidocanol und Derivaten davon
ATE487791T1 (de) Verfahren und formulierung zur extraktion von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien
US20110060135A1 (en) Selective Purification of Small RNAs from Mixtures
ATE501279T1 (de) Formulierungen und verfahren zur isolierung von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien und nachfolgende komplexe genanalytik
RU2265058C2 (ru) Способ выявления в образце нуклеиновой кислоты-мишени (варианты)
DE60327048D1 (de) Verfahren zur in vivo quantifizierung von nukleinsäuren
CN103695419B (zh) 一种病毒核酸提取试剂
US8993292B2 (en) Methods and systems for differential extraction
DE602005012112D1 (de) Verfahren zur kennzeichnung und reinigung von interessierenden nukleinsäuren in einer biologischen probe zur behandlung in einem einzelreaktionsgefäss
Bai et al. Deep sequencing analysis of nucleolar small RNAs: RNA isolation and library preparation
EP3935163A1 (de) Herstellungsverfahren und adaptierte vorrichtung zur filterung von kleinen nukleinsäuren aus biologischen proben