BRPI0915886A2 - método para amplificação direta a partir de amostras brutas de ácido nucléico - Google Patents
método para amplificação direta a partir de amostras brutas de ácido nucléicoInfo
- Publication number
- BRPI0915886A2 BRPI0915886A2 BRPI0915886A BRPI0915886A BRPI0915886A2 BR PI0915886 A2 BRPI0915886 A2 BR PI0915886A2 BR PI0915886 A BRPI0915886 A BR PI0915886A BR PI0915886 A BRPI0915886 A BR PI0915886A BR PI0915886 A2 BRPI0915886 A2 BR PI0915886A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- acid samples
- direct amplification
- crude nucleic
- crude
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7711308P | 2008-06-30 | 2008-06-30 | |
| PCT/US2009/049323 WO2010002938A2 (en) | 2008-06-30 | 2009-06-30 | Method for direct amplification from crude nucleic acid samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0915886A2 true BRPI0915886A2 (pt) | 2015-11-03 |
Family
ID=41466583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0915886A BRPI0915886A2 (pt) | 2008-06-30 | 2009-06-30 | método para amplificação direta a partir de amostras brutas de ácido nucléico |
Country Status (10)
| Country | Link |
|---|---|
| US (4) | US8173401B2 (pt) |
| EP (2) | EP2294225B8 (pt) |
| JP (2) | JP5770085B2 (pt) |
| CN (1) | CN102177250B (pt) |
| BR (1) | BRPI0915886A2 (pt) |
| DK (1) | DK2294225T3 (pt) |
| ES (1) | ES2532632T3 (pt) |
| HR (1) | HRP20150280T1 (pt) |
| SI (1) | SI2294225T1 (pt) |
| WO (1) | WO2010002938A2 (pt) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102177250B (zh) | 2008-06-30 | 2015-05-06 | 生命科技公司 | 由粗制核酸样品直接扩增的方法 |
| EP2625526B1 (en) | 2010-10-04 | 2017-03-15 | Genapsys Inc. | Systems and methods for automated reusable parallel biological reactions |
| US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
| US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
| US9469871B2 (en) | 2011-04-14 | 2016-10-18 | Corporos Inc. | Methods and apparatus for point-of-care nucleic acid amplification and detection |
| US8911941B2 (en) * | 2011-04-14 | 2014-12-16 | Kenneth J. Michlitsch | Methods and apparatus for point-of-care nucleic acid amplification and detection |
| US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
| US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
| DK2718260T3 (en) | 2011-06-08 | 2018-11-19 | Life Technologies Corp | DESIGN AND DEVELOPMENT OF NEW DETERGENTS FOR USE IN PCR SYSTEMS |
| US9567628B2 (en) | 2011-06-08 | 2017-02-14 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| WO2013002354A1 (ja) * | 2011-06-29 | 2013-01-03 | 株式会社ダナフォーム | 生体試料の前処理方法、ｒｎａの検出方法及び前処理キット |
| EP3495507B1 (en) | 2011-07-06 | 2022-03-30 | Quest Diagnostics Investments Incorporated | Direct amplification and detection of viral and bacterial pathogens |
| EP2785868B1 (en) | 2011-12-01 | 2017-04-12 | Genapsys Inc. | Systems and methods for high efficiency electronic sequencing and detection |
| GB201201464D0 (en) | 2012-01-27 | 2012-03-14 | Secr Defence | Analytical method |
| US9354159B2 (en) | 2012-05-02 | 2016-05-31 | Nanoscopia (Cayman), Inc. | Opto-fluidic system with coated fluid channels |
| GB201219137D0 (en) * | 2012-10-24 | 2012-12-05 | Ge Healthcare Uk Ltd | Direct nucleic acid amplification kit, reagent and method |
| CN104769111B (zh) * | 2012-11-09 | 2021-04-27 | 环球生命科学解决方案运营英国有限公司 | 用于非洗提样品的一步法核酸扩增的方法 |
| US9809852B2 (en) | 2013-03-15 | 2017-11-07 | Genapsys, Inc. | Systems and methods for biological analysis |
| JP6559647B2 (ja) * | 2013-03-15 | 2019-08-14 | ジーイー・ヘルスケア・ユーケイ・リミテッド | 非溶出試料のワンステップ核酸増幅方法 |
| CA3156663A1 (en) | 2013-03-15 | 2014-09-18 | Verinata Health, Inc. | Generating cell-free dna libraries directly from blood |
| WO2015061714A1 (en) | 2013-10-25 | 2015-04-30 | Life Technologies Corporation | Novel compounds for use in pcr systems and applications thereof |
| WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
| WO2015096063A1 (en) * | 2013-12-25 | 2015-07-02 | Coyote Bioscience Co., Ltd. | Methods and systems for nucleic acid amplification |
| CN103789299B (zh) * | 2014-01-09 | 2016-08-31 | 东北制药集团辽宁生物医药有限公司 | 一种进行全血基因组快速扩增的方法 |
| EP3556864B1 (en) | 2014-04-18 | 2020-12-09 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
| EP3221704A4 (en) * | 2014-11-20 | 2018-04-11 | GE Healthcare UK Limited | Detecting dementia and alzheimer's disease associated biomarkers stabilized on solid support materials |
| JP2019517780A (ja) * | 2016-04-14 | 2019-06-27 | ティー２ バイオシステムズ，インコーポレーテッド | 複合試料における増幅のための方法及びシステム |
| CN105925692B (zh) * | 2016-05-16 | 2019-09-20 | 安陆市疾病预防控制中心 | 一种常温稳定的pcr预混液 |
| US10544456B2 (en) | 2016-07-20 | 2020-01-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| SG11202002516WA (en) | 2017-09-21 | 2020-04-29 | Genapsys Inc | Systems and methods for nucleic acid sequencing |
| CN107937491A (zh) * | 2017-11-30 | 2018-04-20 | 广州定康医疗科技有限公司 | 一种针对口腔拭子的基于pcr的snp基因分型方法 |
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| US5837453A (en) * | 1992-05-13 | 1998-11-17 | Geron Corporation | Telomerase activity assays |
| EP0590327B1 (en) * | 1992-09-11 | 2003-04-09 | F. Hoffmann-La Roche Ag | Detection of nucleic acids in blood |
| US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| JPH07110455B2 (ja) | 1992-10-27 | 1995-11-29 | 住友電気工業株式会社 | ウェハ固定装置 |
| US6479235B1 (en) | 1994-09-30 | 2002-11-12 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| US5843660A (en) * | 1994-09-30 | 1998-12-01 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| US7008771B1 (en) * | 1994-09-30 | 2006-03-07 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| CA2118048C (en) | 1994-09-30 | 2003-04-08 | James W. Schumm | Multiplex amplification of short tandem repeat loci |
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5861251A (en) * | 1996-10-15 | 1999-01-19 | Bioneer Corporation | Lyophilized reagent for polymerase chain reaction |
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| US6238863B1 (en) * | 1998-02-04 | 2001-05-29 | Promega Corporation | Materials and methods for indentifying and analyzing intermediate tandem repeat DNA markers |
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| US6300069B1 (en) * | 1999-05-03 | 2001-10-09 | Qiagen Gmbh | Generation and amplification of nucleic acids from ribonucleic acids |
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| US20030049614A1 (en) * | 1999-10-06 | 2003-03-13 | Holly Hurlbut Hogrefe | Compositions for dna amplification, synthesis, and mutagenesis |
| EP1351970A2 (en) * | 2000-12-12 | 2003-10-15 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
| US7198897B2 (en) * | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
| CA2533910A1 (en) * | 2003-07-29 | 2005-02-24 | Sigma-Aldrich Co. | Methods and compositions for amplification of dna |
| US20050112591A1 (en) | 2003-11-25 | 2005-05-26 | Applera Corporation | Novel method for isolating single stranded product |
| US7776530B2 (en) * | 2004-06-29 | 2010-08-17 | Wallac Oy | Integrated nucleic acid analysis |
| US20060014190A1 (en) * | 2004-06-30 | 2006-01-19 | Hennessy Lori K | Methods for analyzing short tandem repeats and single nucleotide polymorphisms |
| KR100746372B1 (ko) * | 2005-02-28 | 2007-08-03 | 바이오퀘스트(주) | 핵산 분자가 포함되는 직접 효소 반응 방법 |
| US20080003575A1 (en) * | 2006-06-28 | 2008-01-03 | Sigma-Aldrich Co. | Methods and composition for RNA extraction |
| US20080124768A1 (en) * | 2006-10-31 | 2008-05-29 | Sigma Aldrich Co. | Methods and Compositions for Amplification of DNA |
| US20080138804A1 (en) * | 2006-12-12 | 2008-06-12 | Helicos Biosciences Corporation | Buffer composition |
| WO2009108170A2 (en) * | 2007-11-16 | 2009-09-03 | Invitrogen Corporation | Compositions and methods for determining immune status |
| CN102177250B (zh) | 2008-06-30 | 2015-05-06 | 生命科技公司 | 由粗制核酸样品直接扩增的方法 |
-
2009
- 2009-06-30 CN CN200980134038.5A patent/CN102177250B/zh active Active
- 2009-06-30 DK DK09774388.4T patent/DK2294225T3/en active
- 2009-06-30 SI SI200931149T patent/SI2294225T1/sl unknown
- 2009-06-30 US US12/495,705 patent/US8173401B2/en active Active
- 2009-06-30 EP EP09774388.4A patent/EP2294225B8/en active Active
- 2009-06-30 EP EP14197738.9A patent/EP2905344B1/en active Active
- 2009-06-30 WO PCT/US2009/049323 patent/WO2010002938A2/en active Application Filing
- 2009-06-30 ES ES09774388.4T patent/ES2532632T3/es active Active
- 2009-06-30 BR BRPI0915886A patent/BRPI0915886A2/pt not_active Application Discontinuation
- 2009-06-30 JP JP2011516834A patent/JP5770085B2/ja active Active
-
2012
- 2012-04-03 US US13/438,436 patent/US8460934B2/en active Active
-
2013
- 2013-06-10 US US13/914,478 patent/US9057110B2/en active Active
-
2014
- 2014-06-13 JP JP2014122337A patent/JP2014166189A/ja active Pending
-
2015
- 2015-03-12 HR HRP20150280AT patent/HRP20150280T1/hr unknown
- 2015-06-15 US US14/739,722 patent/US9518302B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2532632T3 (es) | 2015-03-30 |
| EP2905344B1 (en) | 2016-11-09 |
| WO2010002938A3 (en) | 2010-04-08 |
| EP2294225B8 (en) | 2015-02-18 |
| CN102177250B (zh) | 2015-05-06 |
| EP2294225A4 (en) | 2011-10-26 |
| JP2014166189A (ja) | 2014-09-11 |
| US8460934B2 (en) | 2013-06-11 |
| DK2294225T3 (en) | 2015-03-30 |
| US9057110B2 (en) | 2015-06-16 |
| US20130005609A1 (en) | 2013-01-03 |
| JP2011526788A (ja) | 2011-10-20 |
| SI2294225T1 (sl) | 2015-04-30 |
| EP2294225B1 (en) | 2014-12-17 |
| CN102177250A (zh) | 2011-09-07 |
| US9518302B2 (en) | 2016-12-13 |
| US20150354015A1 (en) | 2015-12-10 |
| EP2905344A1 (en) | 2015-08-12 |
| US20100015621A1 (en) | 2010-01-21 |
| JP5770085B2 (ja) | 2015-08-26 |
| WO2010002938A2 (en) | 2010-01-07 |
| US8173401B2 (en) | 2012-05-08 |
| HRP20150280T1 (hr) | 2015-04-10 |
| EP2294225A2 (en) | 2011-03-16 |
| US20140120541A1 (en) | 2014-05-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
| B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |