JP2005511096A5 - - Google Patents
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- Publication number
- JP2005511096A5 JP2005511096A5 JP2003551326A JP2003551326A JP2005511096A5 JP 2005511096 A5 JP2005511096 A5 JP 2005511096A5 JP 2003551326 A JP2003551326 A JP 2003551326A JP 2003551326 A JP2003551326 A JP 2003551326A JP 2005511096 A5 JP2005511096 A5 JP 2005511096A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- annealing
- sequence
- cdna
- mrna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000137 annealing Methods 0.000 claims 151
- 230000003321 amplification Effects 0.000 claims 93
- 238000003199 nucleic acid amplification method Methods 0.000 claims 93
- 239000002299 complementary DNA Substances 0.000 claims 87
- 125000003729 nucleotide group Chemical group 0.000 claims 64
- 239000002773 nucleotide Substances 0.000 claims 63
- 230000000295 complement effect Effects 0.000 claims 54
- 108020004999 messenger RNA Proteins 0.000 claims 54
- 150000007523 nucleic acids Chemical class 0.000 claims 51
- 238000000034 method Methods 0.000 claims 48
- 108020004414 DNA Proteins 0.000 claims 46
- 239000012634 fragment Substances 0.000 claims 38
- 238000004925 denaturation Methods 0.000 claims 36
- 230000036425 denaturation Effects 0.000 claims 36
- 102000039446 nucleic acids Human genes 0.000 claims 34
- 108020004707 nucleic acids Proteins 0.000 claims 34
- 102000053602 DNA Human genes 0.000 claims 24
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 22
- 238000006243 chemical reaction Methods 0.000 claims 21
- 108091034117 Oligonucleotide Proteins 0.000 claims 16
- 230000035772 mutation Effects 0.000 claims 15
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 14
- 108090000623 proteins and genes Proteins 0.000 claims 14
- 108091092584 GDNA Proteins 0.000 claims 12
- 230000001105 regulatory effect Effects 0.000 claims 12
- 102100034343 Integrase Human genes 0.000 claims 8
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 8
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 8
- 230000002255 enzymatic effect Effects 0.000 claims 8
- 238000010839 reverse transcription Methods 0.000 claims 8
- 230000006820 DNA synthesis Effects 0.000 claims 7
- 229940104302 cytosine Drugs 0.000 claims 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 5
- LOJNBPNACKZWAI-UHFFFAOYSA-N 3-nitro-1h-pyrrole Chemical compound [O-][N+](=O)C=1C=CNC=1 LOJNBPNACKZWAI-UHFFFAOYSA-N 0.000 claims 5
- OZFPSOBLQZPIAV-UHFFFAOYSA-N 5-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2NC=CC2=C1 OZFPSOBLQZPIAV-UHFFFAOYSA-N 0.000 claims 5
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical class O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 5
- 229960003786 inosine Drugs 0.000 claims 5
- NEJMFSBXFBFELK-UHFFFAOYSA-N 4-nitro-1h-benzimidazole Chemical compound [O-][N+](=O)C1=CC=CC2=C1N=CN2 NEJMFSBXFBFELK-UHFFFAOYSA-N 0.000 claims 4
- 108020004635 Complementary DNA Proteins 0.000 claims 4
- 108091035707 Consensus sequence Proteins 0.000 claims 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000010804 cDNA synthesis Methods 0.000 claims 4
- 238000002703 mutagenesis Methods 0.000 claims 4
- 231100000350 mutagenesis Toxicity 0.000 claims 4
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 claims 3
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims 3
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims 3
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 3
- 229930010555 Inosine Natural products 0.000 claims 3
- 108700005084 Multigene Family Proteins 0.000 claims 3
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims 3
- WETFHJRYOTYZFD-YIZRAAEISA-N (2r,3s,5s)-2-(hydroxymethyl)-5-(3-nitropyrrol-1-yl)oxolan-3-ol Chemical compound C1[C@H](O)[C@@H](CO)O[C@@H]1N1C=C([N+]([O-])=O)C=C1 WETFHJRYOTYZFD-YIZRAAEISA-N 0.000 claims 2
- NZJKEQFPRPAEPO-UHFFFAOYSA-N 1h-benzimidazol-4-amine Chemical compound NC1=CC=CC2=C1N=CN2 NZJKEQFPRPAEPO-UHFFFAOYSA-N 0.000 claims 2
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 claims 2
- 108091028664 Ribonucleotide Proteins 0.000 claims 2
- 239000005547 deoxyribonucleotide Substances 0.000 claims 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 2
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 claims 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000002336 ribonucleotide Substances 0.000 claims 2
- 125000002652 ribonucleotide group Chemical group 0.000 claims 2
- BCJOBEIODHUZEF-UHFFFAOYSA-N 2-(2-methoxyethyl)-5-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2NC(CCOC)=CC2=C1 BCJOBEIODHUZEF-UHFFFAOYSA-N 0.000 claims 1
- FRYOZNAQLGCIKS-UHFFFAOYSA-N 4-(3-nitro-1h-pyrrol-2-yl)morpholine Chemical compound C1=CNC(N2CCOCC2)=C1[N+](=O)[O-] FRYOZNAQLGCIKS-UHFFFAOYSA-N 0.000 claims 1
- QTJTWXGFPBTKRR-UHFFFAOYSA-N 4-(4-nitro-1h-benzimidazol-2-yl)morpholine Chemical compound N=1C=2C([N+](=O)[O-])=CC=CC=2NC=1N1CCOCC1 QTJTWXGFPBTKRR-UHFFFAOYSA-N 0.000 claims 1
- UTHBAKVGHIUWIP-UHFFFAOYSA-N 4-(5-nitro-1h-indol-2-yl)morpholine Chemical compound C=1C2=CC([N+](=O)[O-])=CC=C2NC=1N1CCOCC1 UTHBAKVGHIUWIP-UHFFFAOYSA-N 0.000 claims 1
- QFFLRMDXYQOYKO-KVQBGUIXSA-N 7-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1h-imidazo[4,5-d]triazin-4-one Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NN=NC(O)=C2N=C1 QFFLRMDXYQOYKO-KVQBGUIXSA-N 0.000 claims 1
- OXYNAKURPIFROL-PYUPQCDSSA-N 9-[(2s,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)-2-morpholin-4-yloxolan-2-yl]-3h-purin-6-one Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@]1(N1C2=NC=NC(O)=C2N=C1)N1CCOCC1 OXYNAKURPIFROL-PYUPQCDSSA-N 0.000 claims 1
- 108700028369 Alleles Proteins 0.000 claims 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 229960005305 adenosine Drugs 0.000 claims 1
- LTTZPKKPMZCXJD-UHFFFAOYSA-N aminophosphonic acid;3-nitro-1h-pyrrole Chemical compound NP(O)(O)=O.[O-][N+](=O)C=1C=CNC=1 LTTZPKKPMZCXJD-UHFFFAOYSA-N 0.000 claims 1
- JRTXBRPKDAKSSH-UHFFFAOYSA-N aminophosphonic acid;4-nitro-1h-benzimidazole Chemical compound NP(O)(O)=O.[O-][N+](=O)C1=CC=CC2=C1NC=N2 JRTXBRPKDAKSSH-UHFFFAOYSA-N 0.000 claims 1
- ATWWOPGVWCAISQ-UHFFFAOYSA-N aminophosphonic acid;5-nitro-1h-indole Chemical compound NP(O)(O)=O.[O-][N+](=O)C1=CC=C2NC=CC2=C1 ATWWOPGVWCAISQ-UHFFFAOYSA-N 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 238000001216 nucleic acid method Methods 0.000 claims 1
- -1 phospho Chemical class 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 238000002741 site-directed mutagenesis Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/KR2001/002133 WO2003050304A1 (en) | 2001-12-08 | 2001-12-08 | Annealing control primer system for regulating primer annealing specificity and its applications |
| KRPCT/KR01/02133 | 2001-12-08 | ||
| KRPCT/KR02/00816 | 2002-05-01 | ||
| PCT/KR2002/000816 WO2003093509A1 (en) | 2002-05-01 | 2002-05-01 | Methods and compositions for improving specificity of pcr amplication |
| PCT/KR2002/001781 WO2003050305A1 (en) | 2001-12-08 | 2002-09-19 | Annealing control primer and its uses |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2005511096A JP2005511096A (ja) | 2005-04-28 |
| JP2005511096A6 JP2005511096A6 (ja) | 2005-08-04 |
| JP2005511096A5 true JP2005511096A5 (enExample) | 2005-12-22 |
| JP4263612B2 JP4263612B2 (ja) | 2009-05-13 |
Family
ID=19198489
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003551326A Expired - Lifetime JP4263612B2 (ja) | 2001-12-08 | 2002-09-19 | アニーリング調節プライマーおよびその使用 |
| JP2003551327A Pending JP2005522190A (ja) | 2001-12-08 | 2002-11-04 | ハイブリダイゼーション部分調節オリゴヌクレオチド及びその用途 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003551327A Pending JP2005522190A (ja) | 2001-12-08 | 2002-11-04 | ハイブリダイゼーション部分調節オリゴヌクレオチド及びその用途 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20030152925A1 (enExample) |
| JP (2) | JP4263612B2 (enExample) |
| KR (1) | KR100649165B1 (enExample) |
| AU (1) | AU2002222737A1 (enExample) |
| CA (1) | CA2469383A1 (enExample) |
| IL (1) | IL162317A0 (enExample) |
| NZ (1) | NZ533221A (enExample) |
| RU (1) | RU2004120769A (enExample) |
| WO (1) | WO2003050304A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280422C (zh) * | 2004-08-26 | 2006-10-18 | 北京博奥生物芯片有限责任公司 | 一种不对称pcr扩增方法及其应用 |
| US20060188910A1 (en) * | 2005-02-18 | 2006-08-24 | Applera Corporation | Compositions, methods, and kits for analyzing DNA methylation |
| WO2007026485A1 (ja) * | 2005-08-30 | 2007-03-08 | Inter-University Research Institute Corporation National Institutes Of Natural Sciences | ユニバーサル塩基 |
| EP2479291A1 (en) * | 2005-10-27 | 2012-07-25 | Life Technologies Corporation | Nucleic acid amplification using non-random primers |
| EP1957645B1 (en) | 2005-12-06 | 2010-11-17 | Ambion Inc. | Reverse transcription primers and methods of design |
| KR100860619B1 (ko) * | 2006-02-23 | 2008-09-29 | 주식회사 씨젠 | 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드 |
| ES2400805T3 (es) * | 2006-05-04 | 2013-04-12 | Seegene, Inc. | Método para amplificar una secuencia de ADN desconocida adyacente a una secuencia conocida |
| WO2008080029A2 (en) | 2006-12-21 | 2008-07-03 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| EP1978104A1 (en) * | 2007-04-03 | 2008-10-08 | Freie Universität Berlin | A method for the quantitative analysis of RNA molecules in a sample |
| KR100987352B1 (ko) | 2008-04-15 | 2010-10-12 | 주식회사 인트론바이오테크놀로지 | 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법 |
| JP2012511927A (ja) | 2008-12-17 | 2012-05-31 | ライフ テクノロジーズ コーポレーション | 対立遺伝子変種を検出するための方法、組成物、およびキット |
| EP3249053A1 (en) | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| JP5687414B2 (ja) | 2009-04-20 | 2015-03-18 | オリンパス株式会社 | 多型の識別方法 |
| EP3150619B1 (en) * | 2009-05-21 | 2019-09-18 | Siemens Healthcare Diagnostics Inc. | Universal tags with non-natural nucleobases |
| EP2449132B1 (en) | 2009-07-01 | 2015-05-13 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| WO2011032053A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
| ES2736977T3 (es) * | 2011-09-14 | 2020-01-09 | Ngk Insulators Ltd | Método para detectar ácido nucleico diana |
| CA2862373C (en) | 2012-04-18 | 2016-07-19 | F. Hoffmann-La Roche Ag | Hev assay |
| KR101525495B1 (ko) * | 2013-11-15 | 2015-06-03 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
| KR101723207B1 (ko) * | 2015-01-29 | 2017-04-05 | (주)다이오진 | 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법 |
| US20180073082A1 (en) * | 2015-02-25 | 2018-03-15 | Diogene Co., Ltd | Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| US10344336B2 (en) | 2015-06-09 | 2019-07-09 | Life Technologies Corporation | Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging |
| KR101961642B1 (ko) * | 2016-04-25 | 2019-03-25 | (주)진매트릭스 | 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 |
| CN111315895A (zh) * | 2017-09-14 | 2020-06-19 | 豪夫迈·罗氏有限公司 | 用于产生环状单链dna文库的新型方法 |
| KR101856205B1 (ko) * | 2017-11-16 | 2018-06-20 | 제노플랜코리아 주식회사 | 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2544292A (en) * | 1991-09-11 | 1993-04-05 | Medical Research Council | Improvements in oligonucleotide primers and probes |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| AU7733200A (en) * | 1999-09-30 | 2001-04-30 | Qiagen Genomics, Inc. | Compositions and methods for reducing oligonucleotide hybridization and priming specificity |
-
2001
- 2001-12-08 WO PCT/KR2001/002133 patent/WO2003050304A1/en not_active Ceased
- 2001-12-08 AU AU2002222737A patent/AU2002222737A1/en not_active Abandoned
- 2001-12-14 US US10/014,496 patent/US20030152925A1/en not_active Abandoned
-
2002
- 2002-09-19 JP JP2003551326A patent/JP4263612B2/ja not_active Expired - Lifetime
- 2002-09-19 IL IL16231702A patent/IL162317A0/xx unknown
- 2002-09-19 KR KR1020047008441A patent/KR100649165B1/ko not_active Expired - Lifetime
- 2002-11-04 CA CA002469383A patent/CA2469383A1/en not_active Abandoned
- 2002-11-04 NZ NZ533221A patent/NZ533221A/en unknown
- 2002-11-04 RU RU2004120769/13A patent/RU2004120769A/ru not_active Application Discontinuation
- 2002-11-04 JP JP2003551327A patent/JP2005522190A/ja active Pending
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