KR20070052890A - 호흡기 바이러스 핵산 검출용 올리고뉴클레오타이드 - Google Patents
호흡기 바이러스 핵산 검출용 올리고뉴클레오타이드 Download PDFInfo
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Abstract
본 발명은 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드, 이를 포함하는 키트 및 이를 사용한 바이러스 증폭 및 검출방법에 관한 것으로서, 종래의 올리고뉴클레오타이드를 사용하여 유전적 다양성을 나타내는 호흡기 바이러스를 검출하는 방법에서 발생할 수 있는 음성-오류뿐만 아니라, 양성-오류 및 배경산물 (backgrounds)의 문제점을 극복할 수 있다. 또한, 멀티플렉스 PCR에서도 우수한 작동성 (workabilty)을 나타내어, 하나의 PCR 반응 세트에서 다양한 종류의 호흡기 바이러스를 동시에 검출할 수 있다.
호흡기 바이러스, 메타뉴모바이러스, 인플루엔자 바이러스, 프라이머, 프로브, 증폭
Description
도 1a-1b는 양성대조군 PCR 결과를 나타낸다. M: 100 bp 래더, 1: 인간 아데노바이러스, 2: 인간 메타뉴모바이러스, 3: 인간 코로나바이러스 229E, 4: 인간 파라인플루엔자 바이러스 1, 5: 인간 파라인플루엔자 바이러스 2, 6: 인간 파라인플루엔자 바이러스 3, S1: 1-6의 혼합 시료, 7: 인플루엔자 A 바이러스, 8: 인플루엔자 B 바이러스, 9: 인간 호흡기 신시티움 바이러스 B, 10: 인간 리노 바이러스, 11: 인간 호흡기 신시티움 바이러스 A, 12: 인간 코로나바이러스 OC43, S2: 7-12의 혼합 시료 (도 1a: 프라이머 세트 I; 및 도 1b 프라이머 세트 II).
도 2a-2c는 24명의 환자로부터 얻은 바이러스 시료에 본 발명의 프라이머 세트 (도 2a: 프라이머 세트 I; 도 2b 프라이머 세트 II; 도 2c 대조용 프라이머 세트)를 사용하여 실시한 멀티플렉스 PCR (multiplex PCR) 결과를 나타낸다. N: 정상인 (음성 대조군), 1-24: 환자 수, P: 양성 대조군, AdV: 인간 아데노바이러스, MPV: 인간 메타뉴모바이러스, CoV229E: 인간 코로나바이러스 229E, PIV1: 인간 파라인플루엔자 바이러스 1, PIV2: 인간 파라인플루엔자 바이러스 2, PIV3: 인간 파라인플루엔자 바이러스 3, INF A: 인플루엔자 A 바이러스, INF B: 인플루엔자 B 바 이러스, RSV B: 인간 호흡기 신시티움 바이러스 B, RSV A: 인간 호흡기 신시티움 바이러스 A, RV: 인간 리노 바이러스, CovOC43: 인간 코로나바이러스 OC43.
본 발명은 호흡기 바이러스 핵산과 혼성화되는 올리고뉴클레오타이드, 이를 포함하는 키트 및 이를 사용한 바이러스 증폭 및 검출방법에 관한 것이다.
인간의 호흡기 질환을 유발하는 것으로 알려진 바이러스로는 다음과 같은 것들이 있다: 인플루엔자 바이러스 A, B 및 C (influenza virus A, B and C: INF A, B and C); 인간 파라인플루엔자 바이러스 1, 2, 3 및 4 (human parainfluenza virus 1, 2, 3 및 4: hPIV 1, 2, 3 and 4); 인간 호흡기 신시티움 바이러스 (human respiratory syncytial virus: hRSV); 인간 메타뉴모바이러스 (human metapneumoviru: hMPV); 인간 코로나바이러스 OC43 (human coronavirus OC43: hCoVOC43) 및 인간 코로나바이러스 229E (human coronavirus 229E: hCoV229E); 인간 리노바이러스 (human rhinovirus: hRV); 아데노바이러스 (adenovirus: AdV); 및 인간 엔테로바이러스 (human enteroviruses: hEV)(S. Bellau-Pujol, et at., Journal of Virological Methods 126 (2005) 53-63).
바이러스를 검출하는 방법으로는 세포배양 방법이나 바이러스 항원 또는 이 의 항체를 검출 방법 등이 알려져 있다 (Gardner, P.S., et al, Br. Med. J. 3 (614), 340-343). 하지만, 이러한 방법들은 소요시간, 편리성 또는 정확성 등을 동시에 만족시킬 수 없으므로 이를 대실할 새로운 방법의 개발이 요구되고 있다.
최근에는 핵산의 혼성화를 이용하여, 바이러스 게놈 또는 이로부터 유래한 RNA 및 DNA를 검출하는 방법이 개발되고 있다.
이 방법은 바이러스 핵산과 혼성화될 수 있는 탐침 (probe)을 사용하여 타깃 바이러스를 검출하거나, 바이러스 유래 핵산을 특이적으로 증폭할 수 있는 프라이머 (primer)를 사용하여 타깃 바이러스를 검출한다.
그런데, 바이러스는 유전적 다양성 (genetic diversity)에 의하여 다양한 변이체가 존재하고, 바이러스 핵산의 양도 소량 존재한다. 따라서, 핵산의 혼성화를 이용하는 방법에 있어서, 정확성 (specificity)과 민감도 (sensitivity)를 충분히 만족시킬 수 있는 탐침 또는 프라이머를 디자인하는 것이 매우 중요하다.
바이러스 게놈에서 서열의 변이가 일어나는 정도는 부위에 따라 다른 것으로 알려져 있다. 따라서, 변이의 정도가 낮은 부위를 기본으로 하여 탐침 및 프라이머가 디자인된다.
현재, PCR 방법으로 호흡기 바이러스를 검출할 수 있는 키트들이 판매되고 있으며, 종래에 사용되던 세포배양법 또는 항원검출법에 비하여 개선된 결과를 제공할 것으로 기대된다.
하지만, PCR을 실시하는 경우, 바이러스의 유전적 다양성에 의하여, 바이러스 핵산이 존재함에 불구하고 사용된 프라이머가 바이러스 핵산과 혼성화되지 않아 결국, 오류-음성 (false-negative) 결과를 나타낼 수 있다. 또한, PCR 수행시 나타날 수 있는 비특이적 산물 (non-specific product)의 문제로 인하여 오류-양성 (false-positive)결과를 나타낼 수 있다.
따라서, 상기와 같은 단점을 극복하고 보다 정확한 결과를 제공할 수 있는 방법이 요구된다.
본 명세서 전체에 걸쳐 다수의 인용문헌 및 특허 문헌이 참조되고 그 인용이 표시되어 있다. 인용된 문헌 및 특허의 개시 내용은 그 전체로서 본 명세서에 참조로 삽입되어 본 발명이 속하는 기술 분야의 수준 및 본 발명의 내용이 보다 명확하게 설명된다.
본 발명의 발명자는 간편하고 신속하면서도 매우 정확하게 호흡기 바이러스를 검출하는 방법을 개발하기 위하여 예의 연구 노력한 결과, 본 발명자에 의하여 발명된 이중 특이성 올리고뉴클레오타이드 (dual specificity oligonucleotide) 형태를 갖는 바이러스 혼성화용 서열을 사용하면, 다양한 호흡기 바이러스를 정확하게 검출할 수 있는 것을 확인하여 본 발명을 완성하였다.
따라서, 본 발명의 목적은 호흡기 바이러스와 혼성화되는 올리고뉴클레오타이드를 제공하는 것이다.
본 발명의 다른 목적은 상기 올리고뉴클레오타이드를 포함하는 호흡기 바이 러스 증폭 또는 검출용 키트를 제공하는 것이다.
본 발명의 또 다른 목적은 상기 올리고뉴클레오타이드를 사용하여 호흡기 바이러스를 증폭하는 방법 또는 검출하는 방법을 제공하는 것이다.
본 발명의 다른 목적 및 이점은 하기의 실시예 및 청구범위에 의해 보다 명확하게 된다.
본 발명의 일 양태에 따르면, 본 발명은 호흡기 바이러스 핵산과 특이적으로 혼성화 (hybridization)되는 올리고뉴클레오타이드에 관한 것이다.
보다 상세하게는, 본 발명은 다음의 일반식으로 표시되는, 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드를 제공한다:
5'-Xp-Yq-Zr-3'
상기 일반식에서, Xp는 혼성화되는 타깃 서열과 실질적으로 상보적인 혼성화 서열을 가지는 5'-고 Tm 특이성 구역 (5'-high Tm specificity portion)이고, Yq는 최소 두 개 이상의 유니버설 염기를 포함하는 분할 구역 (separation portion)이며, Zr은 혼성화되는 타깃 서열과 실질적으로 상보적인 혼성화 서열을 가지는 3'-저 Tm 특이성 구역 (3'-low Tm specificity portion)이고, p, q 및 r은 뉴클레오타이드의 개수를 나타내며, X, Y 및 Z는 디옥시리보뉴클레오타이드 또는 리보뉴클레오타이드이고, 5'-고 Tm 특이성 구역의 Tm은 3'-저 Tm 특이성 구역의 Tm보다 높 으며, 상기 분할 구역은 상기 세 구역 중에서 가장 낮은 Tm을 가지고, 5'-고 Tm 특이성 구역과 3'-저 Tm 특이성 구역이 타깃 서열에 혼성화되는 조건 하에서 상기 분할 구역은 비-염기쌍 버블 구조를 형성하고, 상기 버블 구조는 혼성화 특이성 측면에서 5'-고 Tm 특이성 구역이 3'-저 Tm 특이성 구역으로부터 분할되도록 하며, 이러한 특이성 분할은 DSO 전체 구조의 혼성화 특이성이 5'-고 Tm 특이성 구역과 3'-저 Tm 특이성 구역에 의해 이중적으로 결정도록 하고, 이는 결국 DSO 전체 구조의 혼성화 특이성을 향상시키며, 상기 호흡기 바이러스는 인플루엔자 바이러스, 인간 파라인플루엔자 바이러스, 인간 호흡기 신시티움 바이러스, 인간 메타뉴모바이러스, 인간 코로나바이러스, 인간 리노바이러스 또는 아데노바이러스이며, 상기 타깃 서열은 상기 호흡기 바이러스의 지놈 서열이다.
본 발명은 인플루엔자 바이러스 A 및 B (Influenza virus A and B: INF); 인간 파라인플루엔자 바이러스 1, 2 및 3 (human parainfluenza virus 1, 2 and 3: hPIV); 인간 호흡기 신시티움 바이러스 A 및 B (human respiratory syncytial virus A and B: hRSV A and B); 인간 메타뉴모바이러스 (human metapneumoviru: hMPV); 인간 코로나바이러스 OC43 (human coronavirus OC43: hCoVOC43) 및 인간 코로나바이러스 229E (human coronavirus 229E: hCoV229E); 인간 리노바이러스 (human rhinovirus: hRV); 또는 아데노바이러스 (adenovirus: Adv)의 핵산과 혼성화하는 올리고뉴클레오타이드를 제공한다.
본 명세서에서 용어 “바이러스 핵산”은 바이러스 지놈 자체뿐만 아니라 바이러스 지놈으로부터 유래되는 모든 RNA 또는 DNA (예컨대, cDNA)를 의미한다. 또한, 본 명세서에서 용어 “바이러스 지놈”도 특별히 다르게 언급되지 않는 한, 바이러스 핵산과 동일한 의미로 사용된다.
본 명세서에서 용어“혼성화”는 2개의 단일 가닥 핵산이 상보적인 염기 서열들의 페어링 (pairing)에 의하여 이합체 구조 (duplex structure)를 형성하는 것을 의미한다. 혼성화는 단일 가닥 핵산 서열 간의 상보성이 완전할 경우 (perfect match) 일어나거나 일부 미스매치 (mismatch) 염기가 존재하여도 일어날 수 있다. 혼성화에 필요한 상보성의 정도는 혼성화 반응 조건에 따라 달라질 수 있으며, 특히 온도에 의하여 조절될 수 있다. 일반적으로, 혼성화 온도가 높으면, 완전한 매치일 경우 혼성화가 일어날 가능성이 높으며, 혼성화 온도가 낮으면, 일부 미스매치가 존재하더라고 혼성화가 일어날 수 있다. 혼성화 온도가 낮으면, 낮을수록 혼성화가 일어날 수 있는 미스매치의 정도는 증가할 것이다.
본 발명의 올리고뉴클레오타이드가 가지는 기본적인 구조는 본 발명자에 의해 최초로 제안되는 것이고, 이중 특이성 (dual specificity) 구조라 명명할 수 있고, 이러한 구조를 가지는 올리고뉴클레오타이드는 이중 특이성 올리고뉴클레오타이드 (dual specificity oligonucleotide)라 명명된다. DSO는 본 발명자에 의하여 개발된 신 개념의 올리고뉴클레오타이드로서, 분할 구역에 의해 분리된 5'-고 Tm 특이성 구역 (5'-high Tm specificity portion)과 3'-저 Tm 특이성 구역 (3'-low Tm specificity portion)에 의해 이중적으로 혼성화가 결정되기 때문에 크게 향상된 혼성화 특이성을 나타낼 수 있다.
본 발명의 바람직한 구현예에 따르면, 상기 분할 구역에 위치하는 유니버설 염기는 디옥시이노신, 이노신, 7-디아자-2‘-디옥시이노신, 2-아자-2’-디옥시이노신, 2‘-OMe 이노신, 2'-F 이노신, 디옥시 3-니트로피롤, 3-니트로피롤, 2'-OMe 3-니트로피롤, 2'-F 3-니트로피롤, 1-(2’-디옥시-베타-D-리보푸라노실)-3-니트로피롤, 디옥시 5-니트로피롤, 5-니트로인돌, 2'-OMe 5-니트로인돌, 2'-F 5-니트로인돌, 디옥시 4-니트로벤즈이미다졸, 4-니트로벤즈이미다졸, 디옥시 4-아미노벤즈이미다졸, 4-아미노벤즈이미다졸, 디옥시 네불라린, 2'-F 네불라린, 2'-F 4-니트로벤즈이미다졸, PNA-5-인트로인돌, PNA-네불라린, PNA-이노신, PNA-4-니트로벤즈이미다졸, PNA-3-니트로피롤, 모르포리노-5-니트로인돌, 모르포리노-네불라린, 모르포리노-이노신, 모르포리노-4-니트로벤즈이미다졸, 모르포리노-3-니트로피롤, 포스포라미데이트-5-니트로인돌, 포스포라미데이트-네불라린, 포스포라미데이트-이노신, 포스포라미데이트-4-니트로벤즈이미다졸, 포스포라미데이트-3-니트로피롤, 2'-0-메톡시에틸이노신, 2'-0-메톡시에틸 네불라린, 2'-0-메톡시에틸 5-니트로인돌, 2'-0-메톡시에틸 4-니트로-벤즈이미다졸, 2'-0-메톡시에틸 3-니트로피롤 및 상기 염기의 조합으로 구성된 군으로부터 선택되며, 보다 바람직하게는, 디옥시이노신, 이노신, 1-(2‘-디옥시-베타-D-리보푸라노실)-3-니트로피롤 또는 5-니트로인돌이고, 가장 바람직하게는 디옥시이노신이다.
본 발명의 바람직한 구현예에 따르면, 상기 분할 구역은 유니버설 염기를 가지는 연속된 뉴클레오타이드를 포함한다.
바람직하게는, 상기 5'-고 Tm 특이성 구역의 길이는 3'-저 Tm 특이성 구역보 다 길다. 상기 5'-고 Tm 특이성 구역은 바람직하게는, 15-40 뉴클레오타이드의 길이를 갖는다. 상기 3'-저 Tm 특이성 구역은 바람직하게는, 3-15 뉴클레오타이드의 길이를 갖는다. 상기 분할 구역은 3-10 뉴클레오타이드의 길이를 갖는 것이 바람직하다.
본 발명의 바람직한 구현예에 따르면, 상기 5'-고 Tm 특이성 구역은 40-80℃의 Tm을 갖는다. 바람직하게는, 상기 3'-저 Tm 특이성 구역은 10-40℃의 Tm을 갖는다. 상기 분할 구역은 바람직하게는 3-15℃의 Tm을 갖는다.
본 발명의 바람직한 구현예에 따르면, 본 발명의 호흡기 바이러스-특이 올리고뉴클레오타이드는, (1) 분리 바이러스들 (isolates)에 공통적으로 존재하는 보존서열 부위를 기본으로 하여 (2) 이중 특이성 올리고뉴클레오타이드 (dual specificity oligonucleotide: DSO) 형태로 디자인된다. 본 발명에서 올리고뉴클레오타이드의 제조에 기본이 되는 서열은 공지된 분리 바이러스들 (isolates)의 서열을 검색하여 발굴된 보존서열 부위로서, 발굴된 서열 중 프라이머 또는 프로브로 사용될 수 있으며, DSO 형태로 제조하기에 유용한 서열에서 선택된 것이다.
호흡기 바이러스 지놈에서 보존서열 부위의 선택은 공지의 서열에 기초하여 할 수 있다. 예를 들어, 인플루엔자 바이러스 A의 경우, GenBank 접근번호, NC_004524 H1N1, NC_004907 H9N2, NC_007363 H5N1, NC_007367 H3N2 및 NC_007377 H2N2, 인플루엔자 바이러스 B의 경우, GenBank 접근번호, AF101993, AJ781171, AY044173, AF101999, AF102001, AF102003, AY582055, AY582056, AY504600, AY687393, AY582047, AY582051, AJ781174, AY582052 및 AY582053, 인간 호흡기 신시티움 바이러스 A의 경우, GenBank 접근번호, AY526553, HRSVFG, RSHFUSP, PNRSF0, HRU31559, NC_001803 및 RSH22K, 인간 호흡기 신시티움 바이러스 B의 경우, GenBank 접근번호, AF013254, AF013255, AY353550 D00334, AY526558, AY526559, AY526563, AY526566, AY526561, AY526560, AY526562 및 AY526567, 인간 코로나바이러스 229E의 경우 GenBank 접근번호, hcorona 229e hcorona nl63 (22879), 인간 코로나바이러스 OC43의 경우 GenBank 접근번호, gi50844468, gi329574, gi37702815, gi50844477, gi50844468 및 gi34398245, 인간 메타뉴모바이러스의 경우 GenBank 접근번호, gi42632384, gi42632366, gi42632357, gi56411384, gi42632384 및 gi42632366, 인간 파라인플루엔자 바이러스 1의 경우, GenBank 접근번호, gi1621406, gi1621414, gi1621404, gi1621410, gi1621408, gi332674, gi332673, gi332672, gi332676 및 gi332677, 인간 파라인플루엔자 바이러스 2의 경우, GenBank 접근번호, AB176531, AF533011, AF533012, PAFPIV2HN 및 SNDNPPMF, 인간 파라인플루엔자 바이러스 3의 경우, GenBank 접근번호, AB189960, AB189961, PIFHNA, PIFHNE 및 PIFHNLA, 인간 리노 바이러스의 경우 NC_001617 NC_00149, 인간 아데노바이러스의 경우 HAdV type1, HAdV type2, HAdV type5, HAdV E 및 HAdV B에 기재된 서열을 얼라인먼트하여 보존성 서열을 선택할 수 있다.
이와 같이, 발굴된 보존 서열을 DSO 행태로 디자인한 본 발명의 올리고뉴클레오타이드는 종래의 올리고뉴클레오타이드를 사용한 호흡기 바이러스 검출 시에 문제가 되는 음성-오류 그리고 양성-오류 및 배경 산물 (backgrounds)의 문제를 상당히 개선할 수 있다.
프라이머 또는 프로브를 사용하여 바이러스를 검출하고자 하는 경우 고려해야 할 것이 유전적 다양성 (genetic diversity)이다. 즉, 바이러스의 경우 같은 계통의 분리 바이러스들 (isolates)간에서도 핵산 서열에 차이가 나타난다.
종래에 사용되는 방법은 모든 분리 바이러스 (isolates)에 공통적으로 존재하는 보존서열 부위를 기본으로 하여 최적 서열의 올리고뉴클레오타이드를 제작하고, 상기 올리고뉴클레오타이드와 타깃 바이러스 핵산의 서열 간에 일부 미스매치가 있더라고 혼성화가 될 수 있는 조건 (낮은 엄격조건)에서 반응을 실시하는 것이다.
이렇게 하면, 바이러스의 유전적 다양성에 의하여 발생할 수 있는 음성-오류를 줄일 수 있다. 그러나, 낮은 엄격조건에서 혼성화를 실시하면, 사용되는 프라이머 또는 프로브가 타깃 바이러스 핵산 서열 이외에 다른 비-타깃 서열과 혼성화되는 가능성도 높아져 결국 비-타깃 산물 (non-target product), 즉 양성-오류 (false-positive) 산물 또는 배경산물 (backgrounds)이 발생할 위험성이 높아진다.
물론, 상기 양성-오류 또는 배경산물의 문제를 개선하기 위하여 상대적으로 보다 높은 엄격조건에서 실시하게 되면, 음성-오류 (false-negative) 위험성이 다시 높아진다.
그런데, DSO는 분할 구역 (separation portion)의 존재로 인하여 타깃 서열의 특이성이 이중적으로 조절되어, 상대적으로 낮은 엄격조건에서도 목적으로 하는 타깃 서열에 혼성화되는 특이성은 높으면서도, 비-타깃 서열과 혼성화되는 가능성은 확연히 낮아진다.
본 발명의 올리고뉴클레오타이드는 이와 같은 DSO의 특성이 적용된 것으로, 발굴된 타깃 호흡기 바이러스의 보존 서열을 DSO 행태로 디자인한 본 발명의 올리고뉴클레오타이드를 낮은 엄격조건 하에서 사용하면, 호흡기 바이러스 서열의 유전적 다양성에 의한 음성-오류를 극복하면서도, 비-타깃 서열과의 혼성화로 인한 양성-오류 및 배경 산물 (backgrounds)의 문제점을 고민하지 않아도 된다.
본 발명의 바람직한 구현예에 따르면, 상기 5'-고 Tm 특이성 구역과 3'-저 Tm 특이성 구역은 상기 호흡기 바이러스 지놈의 보존성 서열과 혼성화된다.
본 발명의 바람직한 구현예에 따르면, 상기 분할 구역은 상기 호흡기 바이러스 지놈 타깃 서열에서 유전적 다양성을 보이는 서열과 혼성화된다.
본 발명에 있어서, 프라이머 또는 프로브를 DSO 형태로 제조하는 것은 바이러스 핵산의 유전적 다양성을 극복하는데 있어 다른 이점을 제공한다. 하기의 실시예에서 알 수 있는 바와 같이, 본 발명의 올리고뉴클레오타이드에서 분할구역에 해당하는 유니버설 염기 또는 비-구별성 염기 유사체 구역을 유전적 다양성이 다양하게 나타나는 타깃 바이러스 핵산 서열 부위와 매치되도록 디자인하는 경우에는, 호흡기 바이러스의 유전적 다양성을 효율적으로 극복할 수 있다.
본 발명의 바람직한 구현예에 따르면, 본 발명의 올리고뉴클레오타이드는 분할구역 이외에도 유전적 다양성이 나타나는 염기 위치에 유니버설 염기 또는 비-구별성 염기 유사체가 위치하도록 디자인될 수 있다.
본 발명의 바람직한 구현예에 따르면, 본 발명의 올리고뉴클레오타이드는 유전적 다양성이 나타나는 염기에 해당하는 각각의 염기로 디자인될 수 있다. 예를 들어, 분리 바이러스들이 특정 염기 위치에서 A 또는 T를 나타내는 경우, 해당 위치의 염기가 A 또는 T인 올리고뉴클레오타이드 2 종으로 제조될 수 있다.
본 발명의 가장 바람직한 구현예에 따르면, 본 발명의 올리고뉴클레오타이드는 상기 타깃 바이러스 핵산에 특이적으로 혼성화되는 것으로 서열목록 제 12 내지 37 서열에서 선택되는 올리고뉴클레오타이드이다.
서열목록 제 12 서열 내지 37 서열과 그 타깃 바이러스는 표 1과 같다.
SEQ-ID | 명칭 | 서열 (5’→ 3’) | 바이러스 |
12 | INFa-F85 | AGGCCCCCTCAAAGCCGAGAIIIIICAGAGACTTG | INF A |
13 | INFa-R598 | ATAGCCTTAGCTGTAGTGCTGGCIIIIICCATTCTGTT | |
14 | INFB-5'-955 | TTGGCTATGACIGAAAGIATAACCIIIIICAGCCCAA | INF B |
15 | INFB-3'-1409 | TTACATGTTCGGTAAAAITCGTTTAIIIIITCCATACATG | |
16 | PIV1-HN-F1 | GACCCACATGATTTCTGGAGATGIIIIITAGGAGAACC | hPIV 1 |
17 | PIV1-HN-R1 | CTATTACAGAACATGATTTCCTGTTGTCIIIIITGTCATAGGT | |
18 | hPIV-2-5' | TGGACCTCTCCTAAATATICCCAGCIIIIICCCCTCAGCA | hPIV 2 |
19 | hPIV-2-3' | GTGACTGAACAGCTTTTGCGATTGIIIIIATCACTTAGG | |
20 | hPIV-3-5' | TAAGIATAAAATGGACATGGCATAAIIIIITATCAAGACC | hPIV 3 |
21 | hPIV-3-3' | CGTTTACICTTTCIGTTGCTGTTGAGIIIIITATGACTGGG | |
22 | RSVa-F103 | AGAATTTTATCAATCAACATGCAGTGIIIIIAGCAAAGGCT | hRSV A |
23 | RSVa-R382 | ATTGTTGAGTGTATAATTCATAAACCTTGGIIIIICTCTTCTGGC | |
24 | RSVb-F536 | TCAGTCTATCAAATGGGGTCAGTGIIIIIACCAGCAAAG | hRSV B |
25 | RSVb-R927 | ATTACACCATAGATAGGTAGCTGTACAACIIIIICAAGGACTTC | |
26 | hMPV-5'-585 | AGCTTCAGTCAATTCAACAGAAIIIIICTAAATGTTG | hMPV |
27 | hMPV-3'-1007 | TTGAITGCTCAGCIACATTAATIIIIICWGCTGTGTC | |
28 | hCoVOC43-F400 | TATGTTAGGCCGATAATTGAGGACTIIIIIACTCTGACGG | hCoVOC43 |
29 | hCoVOC43-3' | GTAATTACCGACTTTGGACTTAACATIIIIIGCAAAACCAC | |
30 | hCo229E-F375 | TICTTAAGCAGTATACTTCTGCTTGTIIIIICTATTGAAGA | hCoV299E |
31 | hCo229E-R375 | TGGCCATICGTTCAGCATCAGCGAIIIIIGGCAAAACCA | |
32 | hRV-F151 | CCCCACGGGTGACCGTGTCCCAIIIIICGTGGCGGCC | hRV |
33 | hRV-R151 | GCTCCAGGGTTAAGGTTAGCCGCIIIIIGGGGCCGG | |
34 | hAdV-F1 | TCTAIGGCATCTCGATCCAGCAIIIIICCTCGTTTCG | hAdv |
35 | hAdV-R1 | GGACACIIGCTCATGGAIACCARAGIIIIIAAACGCATCAA | |
36 | hRVa-F15 | GAAACACGGACACCCAAAGTIIIIIGTCCCRTCCC | hRV |
37 | hRV-R353 | ATCGTTAICCGCAAAGTGCCTIIIIIAAGCYTAGTA |
* I: 디옥시이노신
R: A 또는 G
W: A 또는 T
Y: C 또는 T
본 발명의 올리고뉴클레오타이드는 서열목록 제 12 서열 내지 37 서열뿐만 아니라 상기 서열과 상보적인 서열 및 실질적으로 동일한 서열을 포함한다. 본 명세서에서 용어 “실질적으로 동일한 서열”은 올리고뉴클레오타이드가 상기한 DSO의 구조를 가지면서 타깃 서열에 특이적으로 혼성화되는 능력을 보유하는 범위 내에서, 서열목록 제 12 서열 내지 37 서열에 기재된 서열의 일부 염기가 결실, 부가 및/또는 치환된 서열을 의미한다. 이러한 실질적으로 동일한 서열이 본 발명의 청구범위에 속한다는 것은 균등론의 원칙 하에 명확하게 인식된다.
본 발명에 있어서, 용어 '타깃 서열 (target sequence)' 은 주어진 혼성화 조건 하에서 사용되는 프라이머 또는 프로브와 혼성화되는 것이 소망되는 서열을 의미한다. 바람직하게는, 타깃 서열은 서열목록 제1서열 내지 제11서열에 기재된 서열이다.
한편, 용어 ‘비-타깃 서열 (non-target sequence)’은 상기 ‘타깃 서열’이외의 서열을 말한다.
예를 들어, 프라이머 또는 프로브의 서열과 완벽하게 일치하는 서열에만 혼성화시키는 것을 소망하여, 고엄격조건 하에서 혼성화를 실시하는 경우, 프라이머 또는 프로브의 서열과 완벽하게 일치하는 서열은 ‘타깃 서열’이 되나 프라이머 또는 프로브의 서열과 하나의 염기라도 다른 서열은 ‘비-타깃 서열’또는 ‘비-특이적 서열 (non-specific sequence)’이 된다. 그리고, 비-타깃 서열과 혼성화의 결과로 얻어지는 산물은 ‘비-타깃 산물’또는 ‘비-특이적 산물’이 된다.
한편, 프라이머 또는 프로브의 서열과 완벽하게 일치하는 서열뿐만 아니라 일부 미스매치가 있는 서열에도 혼성화시키는 것을 소망하여, 낮은 엄격조건 하에서 혼성화를 실시하는 경우, 프라이머 또는 프로브의 서열과 완벽하게 일치하지 않더라도 주어진 조건에서 사용되는 프라이머 또는 프로브와 혼성화되는 것이 소망되는 서열은 ‘타깃 서열’이 되나 그 이외의 서열은‘비 타깃-서열’이 된다.
본 발명의 일 구현예에 따르면, 본 발명의 올리고뉴클레오타이드는 프로브 (probe)로서 타깃 바이러스 핵산과의 혼성화를 통하여 타깃 바이러스 핵산을 검출하는데 사용된다. 본 명세서에서 용어 “프로브”는 단일쇄 핵산 분자이며, 타깃 핵산 서열에 상보적인 서열을 포함한다. 본 발명의 일 구현예에 따르면, 본 발명의 프로브의 이점, 즉 혼성화 특이성의 개선이 손상되지 않는 범위 내에서, 본 발명의 올리고뉴클레오타이드를 변형할 수 있다. 이들 변형, 즉 표지는 혼성화 여부를 검출케 하는 시그널을 제공할 수 있으며, 이는 올리고뉴클레오타이드에 연결될 수 있다. 적합한 표지는 형광단, 발색단, 화학발광단, 자기입자, 방사능동위원소, 매스 표지, 전자밀집입자, 효소, 조인자, 효소에 대한 기질, 그리고 항체, 스트렙타비딘, 바이오틴, 디곡시게닌과 킬레이팅기와 같은 특정 결합 파트너를 갖는 햅텐을 포함하나, 이에 한정되는 것은 아니다. 표지는 형광, 방사능, 발색 측정, 중량 측정, X-선 회절 또는 흡수, 자기, 효소적 활성, 매스 분석, 결합 친화도, 혼성화 고주파, 나노크리스탈에 의하여 검출할 수 있는 시그널을 제공한다. 본 발명의 올리고뉴클레오타이드는 5‘-말단, 3’-말단 또는 내부에 표지된다. 표지는 직접 표지 즉, 염료 또는 간접 표지 즉, 바이오틴, 디고신 (digoxin), 알칼리 포스파타아제, 호스래디쉬 퍼옥시다아제 등일 수 있다.
본 발명의 일 구현예에 따르면, 본 발명의 올리고뉴클레오타이드는 불용성 담체(예컨대, 니트로셀룰로오스 또는 나일론 필터, 유리판, 실리콘 및 플루오로카본 지지체)에 고정화되어, 마이크로어레이를 제조할 수 있다. 마이크로어레이에 있어서 본 발명의 올리고뉴클레오타이드는 혼성화 어레이 요소 (hybridizable array element)로서 이용된다. 불용성 담체에로의 고정화는 화학적 결합 방법 또는 UV와 같은 공유 결합적 방법에 의해 실시된다. 본 발명의 구체적인 일 실시예에 따르면, 상기 혼성화 어레이 요소는 에폭시 화합물 또는 알데히드기를 포함하도록 변형된 글래스 표면에 결합될 수 있고, 또한 폴리라이신 코팅 표면에서 UV에 의해 결합될 수 있다. 또한, 상기 혼성화 어레이 요소는 링커 (예: 에틸렌 글리콜 올리고머 및 디아민)를 통해 담체에 결합될 수 있다.
본 발명의 일 구현예에 따르면, 본 발명의 올리고뉴클레오타이드는 프라이머 (primer)로서 타깃 바이러스 핵산과의 혼성화를 통하여 타깃 바이러스 핵산을 증폭하는데 사용된다. 본 명세서에서 용어 “프라이머”는 합성 또는 자연의 올리고뉴클레오타이드를 의미한다. 프라이머는 주형에 상보적인 프라이머 연장 산물의 합성이 유도되는 조건, 즉, 뉴클레오타이드와 DNA 중합효소와 같은 중합체의 존재, 그리고 적합한 온도와 pH의 조건에서 합성의 개시점으로 작용한다. 증폭의 최대 효율을 위하여, 바람직하게는 프라이머는 단일쇄이다. 바람직하게는, 프라이머는 디옥시리보뉴클레오타이드이다. 본 발명의 프라이머는 자연 (naturally occurring) dNMP (즉, dAMP, dGMP, dCMP 및 dTMP), 변형 뉴클레오타이드 또는 비-자연 뉴클레오타이드를 포함할 수 있다. 또한, 프라이머는 리보뉴클레오타이드도 포함할 수 있다. 예컨대, 본 발명의 올리고뉴클레오타이드는 골격 변형된 뉴클레오타이드 예컨대, 펩타이드 핵산 (PNA) (M. Egholm et al., Nature, 365:566-568(1993)), 포스포로티오에이트 DNA, 포스포로디티오에이트 DNA, 포스포로아미데이트 DNA, 아마이드-연결된 DNA, MMI-연결된 DNA, 2'-O-메틸 RNA, 알파-DNA 및 메틸포스포네이트 DNA, 당 변형된 뉴클레오타이드 예컨대, 2'-O-메틸 RNA, 2'-플루오로 RNA, 2'-아미노 RNA, 2'-O-알킬 DNA, 2'-O-알릴 DNA, 2'-O-알카이닐 DNA, 헥소스 DNA, 피라노실 RNA 및 안히드로헥시톨 DNA, 및 염기 변형을 갖는 뉴클레오타이드 예컨대, C-5 치환된 피리미딘 (치환기는 플루오로-, 브로모-, 클로로-, 아이오도-, 메틸-, 에틸-, 비닐-, 포르밀-, 에티틸-, 프로피닐-, 알카이닐-, 티아조릴-, 이미다조릴-, 피리딜- 포함), C-7 치환기를 갖는 7-데아자퓨린 (치환기는 플루오로-, 브로모-, 클로로-, 아이오도-, 메틸-, 에틸-, 비닐-, 포르밀-, 알카이닐-, 알켄일-, 티아조릴-, 이미다조릴-, 피리딜-), 이노신 및 디아미노퓨린을 포함할 수 있다.
프라이머의 서열은 주형의 일부 서열과 완전하게 상보적인 서열을 가질 필요는 없으며, 주형과 혼성화 되어 프라이머 고유의 작용을 할 수 있는 범위 내에서의 충분한 상보성을 가지면 충분하다.
본 발명의 일 구현예에 따르면, 본 발명의 올리고뉴클레오타이드를 사용한 타깃 바이러스 핵산의 증폭은 타깃 바이러스의 검출을 위한 것이다.
본 발명의 다른 양태에 따르면, 본 발명은 본 발명의 올리고뉴클레오타이드로 구성되는 호흡기 바이러스 핵산과 혼성화하는 올리고뉴클레오타이드 쌍 (pair)을 제공한다.
바람직한 올리고뉴클레오타이드 쌍은 서열목록 제12 및 13서열, 서열목록 제 14 및 15 서열, 서열목록 제 16 및 17 서열, 서열목록 제 18 및 19 서열, 서열목록 제 20 및 21 서열, 서열목록 제 22 및 23 서열, 서열목록 제 24 및 25 서열, 서열목록 제 26 및 27 서열, 서열목록 제 28 및 29 서열, 서열목록 제 30 및 31 서열, 서열목록 제 32 및 33 서열 (또는 서열목록 제 36 및 37 서열), 및 서열목록 제 34 및 35 서열로 이루어진 쌍이다.
본 발명의 올리고뉴클레오타이드 쌍은 상술한 본 발명의 올리고뉴클레오타이드 서열로 구성되므로, 본 명세서의 과도한 복잡성을 피하기 위하여, 중복된 내용은 그 기재를 생략한다.
바람직하게는 본 발명의 올리고뉴클레오타이드 쌍은 호흡기 바이러스 검출을 위한 프라이머 쌍 (전방형 및 역방향 프라이머)으로 사용된다.
본 발명의 또 양태에 따르면, 본 발명은 상기 올리고뉴클레오타이드 쌍 (pair)에서 선택되는 두 쌍 이상의 올리고뉴클레오타이드 쌍을 포함하는 호흡기 바이러스 핵산과 혼성화되는 올리고뉴클레오타이드 세트 (set)를 제공한다.
본 발명의 올리고뉴클레오타이드 세트는 상술한 본 발명의 올리고뉴클레오타이드로 구성되므로, 본 명세서의 과도한 복잡성을 피하기 위하여, 중복된 내용은 그 기재를 생략한다.
본 발명의 올리고뉴클레오타이드 세트는 호흡기 바이러스 검출을 위한 프로브 세트 또는 증폭을 위한 프라이머 세트로 사용될 수 있다. 본 발명의 올리고뉴클레오타이드는 목적에 따라 동시에 여러 종류가 사용될 수 있으며, 동시에 사용되어도 각각 및 전체 결과에 영향을 미치지 않는다.
본 발명의 바람직한 구현예에 따르면, 본 발명의 올리고뉴클레오타이드 세트는 멀티플렉스 PCR (multiplex PCR)의 프라이머 세트로 사용된다.
본 발명의 바람직한 구현예에 따르면, 서열목록 제 16 및 17 서열, 서열목록 제 18 및 19 서열, 서열목록 제 20 및 21 서열, 서열목록 제 26 및 27 서열, 서열목록 제 30 및 31 서열, 및 서열목록 제 34 및 35 서열을 한 세트로 하여 사용한다.
본 발명의 바람직한 구현예에 따르면, 서열목록 제 12 및 13 서열, 서열목록 제 14 및 15 서열, 서열목록 제 22 및 23 서열, 서열목록 제 24 및 25 서열, 서열목록 제 28 및 29 서열, 그리고 서열목록 제 36 및 37 서열을 한 세트로 하여 사용한다.
본 발명의 다른 양태에 따르면, 본 발명의 올리고뉴클레오타이드, 올리고뉴클레오타이드 쌍, 또는 올리고뉴클레오타이드 세트를 포함하는 호흡기 바이러스 검출용 키트 또는 호흡기 바이러스 증폭용 키트를 제공한다.
본 발명은 상술한 본 발명의 올리고뉴클레오타이드를 포함하므로, 본 명세서의 과도한 복잡성을 피하기 위하여, 중복된 내용은 그 기재를 생략한다.
본 발명의 일 구현예에 따르면, 본 발명의 키트가 PCR에 사용되는 경우, 선택적으로 PCR 반응에 필요한 시약, 예컨대, 완충액, DNA 중합효소, DNA 중합효소 조인자 및 디옥시리보뉴클레오타이드-5'-트리포스페이트를 포함할 수 있다. 선택적으로, 본 발명의 키트는 다양한 폴리뉴클레오타이드 분자, 역전사 효소, 다양한 완충액 및 시약, DNA 중합효소의 활성을 억제하는 저해제를 포함할 수 있다.
또한, 본 발명의 키트는 포지티브 및 네가티브 대조군 반응을 수행하는 데 필요한 시약 또는 혼성화 반응에 필요한 완충액과 같은 시약을 포함할 수 있다. 특정 반응에서 이용되는 시약의 최적양은 본 명세서의 내용을 파악한 당업자에 의해 용이하게 결정될 수 있다. 전형적으로, 본 발명의 키트는 분리된 패키지 또는 구획에 상술한 성분들을 포함한다.
본 발명의 다른 양태에 따르면, 본 발명은 상기 올리고뉴클레오타이드, 올리고뉴클레오타이드 쌍, 올리고뉴클레오타이드를 세트를 사용하여 상기 올리고뉴클레오타이드를 호흡기 바이러스 핵산에 혼성화시키는 단계를 포함하는 호흡기 바이러스 검출방법을 제공한다.
본 발명의 또 다른 양태에 따르면, 본 발명은 상기 올리고뉴클레오타이드, 올리고뉴클레오타이드 쌍, 올리고뉴클레오타이드를 세트를 사용하여 상기 올리고뉴클레오타이드를 호흡기 바이러스 핵산에 혼성화시키는 단계를 포함하는 호흡기 바이러스 증폭방법을 제공한다.
본 발명에서, 적합한 혼성화 조건은 최적화 절차에 의하여 일련의 과정으로 결정될 수 있다. 이런 절차는 연구실에서 사용을 위한 프로토콜을 수립하기 위하여 당업자에 의하여 일련의 과정으로 실시된다. 예를 들어, 온도, 성분의 농도, 혼성화 및 세척 시간, 완충액 성분 및 이들의 pH 및 이온세기 등의 조건은 올리고뉴클레오타이드의 길이 및 GC 양 및 타깃 뉴클레오타이드 서열 등의 다양한 인자에 의존한다. 혼성화를 위한 상세한 조건은 Joseph Sambrook, et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(2001); 및 M.L.M. Anderson, Nucleic Acid Hybridization, Springer-Verlag New York Inc. N.Y.(1999)에서 확인할 수 있다.
본 발명의 바람직한 구현예에 따르면, 혼성화 온도는 40℃ 내지 70℃이고 보다 바람직하게는 45℃ 내지 68℃이고, 가장 바람직하게는 50℃ 내지 65℃이다.
호흡기 바이러스의 핵산 서열을 증폭하는 본 발명의 방법은 당업계에 공지된 다양한 프라이머-관련 핵산 증폭 방법들에 따라 실시될 수 있다.
핵산 서열을 증폭하기 위한 본 발명의 방법은 소망하는 어떠한 호흡기 바이러스의 핵산 분자의 증폭에도 이용될 수 있다. 이러한 핵산 분자는 DNA 또는 RNA이다. 상기 핵산 분자는 이중쇄 또는 단일쇄일 수 있고, 바람직하게는 이중쇄이다. 출발물질로서 핵산이 이중쇄인 경우, 두 쇄를 단일쇄로, 또는 부분적인 단일쇄 형태로 만드는 것이 바람직하다. 쇄를 분리하는 방법은, 열, 알카리, 포름아미드, 우레아 및 글리콕살 처리, 효소적 방법(예, 헬리카아제 작용) 및 결합 단백질을 포함하나, 이에 한정되는 것은 아니다. 예를 들어, 쇄 분리는 80-105℃의 온도로 열 처리하여 달성될 수 있다. 상술한 처리의 일반적인 방법은 Joseph Sambrook, 등, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(2001)에 개시되어 있다.
mRNA가 출발물질로 이용되는 경우, 역전사 단계가 증폭 전에 필요로 하며, 역전사 단계의 상세한 내용은 Joseph Sambrook, 등, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(2001) 및 Noonan, K. F. et al., Nucleic Acids Res. 16:10366 (1988)에 개시되어 있다. 역전사 단계에서, mRNA의 폴리 A 테일에 혼성화되는 올리고뉴클레오타이드 dT 프라이머가 이용된다. 올리고뉴클레오타이드 dT 프라이머는 dTMPs로 이루어져 있고, dT 프라이머가 프라이머로서 작용할 수 있는 한도 내에서 dTMPs 중 하나 또는 그 이상은 다른 dNMPs로 대체될 수 있다. 역전사 단계는 RNase H 활성을 갖는 역전사 효소에 의해 이루어질 수 있다. RNase H 활성을 갖는 효소를 이용하는 경우, 반응조건을 주의하여 선택하면 개별적인 RNase H 절단 과정을 피할 수 있다.
본 발명에 이용되는 프라이머는 주형의 한 부위에 혼성화 또는 어닐링되어, 이중쇄 구조를 형성한다. 이러한 이중쇄 구조를 형성하는 데 적합한 핵산 혼성화의 조건은 Joseph Sambrook, 등, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(2001) 및 Haymes, B. D., 등, Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, D.C. (1985)에 개시되어 있다.
다양한 DNA 중합효소가 본 발명의 증폭 단계에 이용될 수 있으며, E. coli DNA 중합효소 I의 “클레나우” 단편, 열안정성 DNA 중합효소 및 박테리오파아지 T7 DNA 중합효소를 포함한다. 바람직하게는, 중합효소는 다양한 박테리아 종으로부터 얻을 수 있는 열안정성 DNA 중합효소이고, 이는 Thermus aquaticus(Taq), Thermus thermophilus(Tth), Thermus filiformis, Thermis flavus, Thermococcus literalis, 및 Pyrococcus furiosus(Pfu)를 포함한다. 상기 중합효소 대부분은 박테리아 그 자체로부터 분리될 수 있고 또는 상업적으로 구입할 수 있다. 또한, 본 발명에 이용되는 중합효소는 중합효소를 암호화하는 클로닝 유전자의 높은 레벨을 발현하는 세포로부터 수득할 수 있다.
중합 반응을 실시할 때, 반응 용기에 반응에 필요한 성분들을 과량으로 제공하는 것이 바람직하다. 증폭 반응에 필요한 성분들의 과량은, 증폭반응이 성분의 농도에 실질적으로 제한되지 않는 정도의 양을 의미한다. Mg2 +와 같은 조인자, dATP, dCTP, dGTP 및 dTTP를 소망하는 증폭 정도가 달성될 수 있을 정도로 반응 혼합물에 제공하는 것이 소망된다.
증폭 반응에 이용되는 모든 효소들은 동일한 반응 조건에서 활성 상태일 수 있다. 사실, 완충액은 모든 효소들이 최적의 반응 조건에 근접하도록 한다. 따라서, 본 발명의 증폭 과정은 반응물의 첨가와 같은 조건의 변화 없이 단일 반응물에서 실시될 수 있다.
본 발명에 있어서 어닐링 또는 혼성화는 타깃 뉴클레오타이드 서열과 프라이머 사이에 특이적 결합을 가능하게 하는 엄격조건 (즉, 분할 구역이 타깃 서열에 수소결합을 할 수 없는 조건) 하에서 실시된다. 어닐링을 위한 엄격조건은 서열-의존적이며 주위 환경적 변수에 따라 다양하다. 바람직하게는, 어닐링 온도는 40℃ 내지 70℃이고 보다 바람직하게는 45℃ 내지 68℃이고, 가장 바람직하게는 50℃ 내지 65℃이다.
본 발명의 가장 바람직한 구현예에서, 증폭 과정은 미국특허 제4,683,195호, 제4,683,202호 및 제4,800,159호에 개시된 PCR에 따라 실시된다.
본 발명의 방법은 특정 목적을 위한 공지의 다른 과정과 결합될 수 있다. 예를 들어, 증폭 산물의 분리(또는 정제)는 증폭과정에 후속될 수 있다. 상기 과정은 겔 전기영동, 컬럼 크로마토그래피, 친화성 크로마토그래피 또는 혼성화에 의해 실시될 수 있다. 또한, 본 발명의 증폭 산물은 클로닝용 담체에 삽입될 수 있다. 또한, 본 발명의 증폭 산물은 발현 벡터를 가지는 적합한 숙주에서 발현될 수 있다. 증폭 산물을 발현하기 위하여, 프로모터의 조절하 또는 프로모터에 작동적으로 연결된 증폭 산물을 운반하는 발현 벡터를 제조한다. 다양한 숙주-발현 시스템에서 단백질 또는 펩타이드 발현을 하기 위하여, 증폭산물 및 전사/번역/조절 서열을 포함하는 발현 벡터를 제작하는 많은 표준 기술들이 알려져 있다. 원핵세포 숙주용 프로모터는 pL λ프로모터, trp 프로모터, lac 프로모터 및 T7 프로모터를 포함하나, 이에 한정되는 것은 아니다. 진핵세포 숙주용 프로모터는 메탈로티오네인 프로모터, 아데노바이러스 후기 프로모터, 백시니아 바이러스 7.5K 프로모터, 그리고 폴리오마, 아데노바이러스 2, 시미안 바이러스 40 또는 시토메갈로 바이러스로부터 유래된 프로모터를 포함하나, 이에 한정되는 것은 아니다. 원핵세포 숙주의 예는 E. coli, 바실러스 서브틸리스, 그리고, 살로넬라 티피무리움, 세라티아 마르세센스 및 다양한 슈도모나스 종과 같은 장내세균을 포함한다. 미생물뿐만 아니라, 다세포 유기체로부터 유래된 세포 배양물도 숙주로서 이용할 수 있다. 척추동물 또는 무척추동물로부터 유래된 세포배양물이 이용될 수 있다. 포유동물 세포뿐만 아니라, 재조합 바이러스 발현 벡터(예컨대, 배큘로바이러스)로 감염된 곤충세포 시스템; 및 재조합 바이러스 발현 벡터(예컨대, 콜리플라우어 모자이크 바이러스, 담배 모자이크 바이러스)로 감염되거나 하나 또는 그 이상의 암호화 서열을 포함하는 재조합 플라스미드 발현 벡터(예컨대, Ti 플라스미드)로 형질전환된 식물도 이용될 수 있다. 증폭산물로부터 발현된 폴리펩타이드는 당업계에 공지된 방법에 따라 정제될 수 있다.
한편, 본 발명의 방법에 있어서, 바람직하게는, 본 발명은 멀티플렉스 PCR 과정에 따라 실시된다. 멀티플렉스 PCR은 PCR의 다른 변형 형태로서, 동일한 반응물에서 둘 이상의 프라이머쌍을 이용하여 하나 이상의 타깃 서열을 동시에 증폭할 수 있다. 그러나, 멀티플렉스 PCR로부터 얻은 결과는, 증폭 과정의 인위적 요소 때문에 종종 복잡하게 나오며, 이러한 오류의 결과는 반응 실패에 의한 위음 결과 그리고 가짜 생성물의 증폭과 같은 위양 결과를 포함한다. 따라서 이러한 오류를 줄이기 위하여 멀티플렉스 PCR 반응 조건을 최적화하는 데 인력과 시간을 많이 소비하지만, 만족할만한 결과를 얻기는 극히 힘들다. 본 발명의 방법은 이러한 종래의 멀티플렉스 PCR의 문제점을 극복하여, 하나의 PCR 반응 세트에서 다양한 종류의 호흡기 바이러스를 동시에 오류 없이 증폭할 수 있다.
본 발명의 이점 (advantages)을 정리하면 다음과 같다: (a) 호흡기 바이러스 (대체적으로 RNA 바이러스)는 지놈 상에서 변이가 많이 되어 유전적 다양성을 나타내는데, 본 발명의 올리고뉴클레오트이드는 상대적으로 낮은 엄격 조건 하에서도 목적으로 하는 타깃 서열에만 혼성화되고 비-타깃 서열에는 혼성화되지 않으므로, 유전적 다양성을 갖는 호흡기 바이러스 검출시 문제되는 음성-오류 및 양성-오류를 극복할 수 있다;
(b) 본 발명의 올리고뉴클레오타이드는 멀티플렉스 PCR에서 우수한 작동성 (workability)을 나타내어, 하나의 PCR 반응 세트에서 다양한 종류의 호흡기 바이러스를 동시에 검출할 수 있다.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.
실시예
실시예
I:
프라이머
디자인 및 제조
이용 가능한 분리 바이러스들 (virus isolates)의 서열을 비교하여 보존서열 부위를 발굴하였으며, 이 서열들 중 본 발명의 프라이머를 제조하는 데 적합한 서열을 선택하여 사용하였다. 서열에서 I는 디옥시이노신을 나타낸다.
실시예
I-1: 인플루엔자 바이러스 A (
INF
A) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 인플루엔자 바이러스 A의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 인플루엔자 바이러스 A의 서열들을 비교하여 보존서열을 발굴하였다. 발굴된 서열 중 본 발명의 DSO (dual specificity oligonucleotide) 개념의 프라이머를 디자인하는데 적합한 서열을 사용하여 전방향 (forward) 및 역방향 (reverse) 프라이머를 디자인하였으며, 유전적 다양성 (genetic diversity)이 나타나는 부분에 DSO의 특징중 하나인 분할 구역 (separation portion) (IIIII)이 위치하도록 디자인하였다.
특히, 인플루엔자 바이러스 A 증폭용 프라이머는 인간뿐만 아니라 조류 인플루엔자 바이러스 (H5N1 및 H9N2)를 증폭할 수 있도록 디자인되었다. 하기의 번호는 NCBI의 GenBank의 접근번호 (accession no.)이며, 밑줄친 부분은 유전적 다양성을 보이는 서열이다:
NC_004524 H1N1 ---AGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG---
NC_004907 H9N2 ---AGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG---
NC_007363 H5N1 ---AGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG---
NC_007367 H3N2 ---AGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG---
NC_007377 H2N2 ---AGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG---
INFA
-F85 primer 5'-
AGGCCCCCTCAAAGCCGAGAIIII
I
CAGAGACTTG
-3' (
SEQ
ID NO: 12)
NC_004524 H1N1 ---ATAGCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTT---
NC_004907 H9N2 ---ATAGCCTTAGCTGTAGTGCTGGCTAGTACCATTCTGTT---
NC_007363 H5N1 ---ATAGCCTTAGCTGTAGTGCTGGCCAGCACCATTCTGTT---
NC_007367 H3N2 ---ATAGCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTT---
NC_007377 H2N2 ---ATAGCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTT---
INFA
-
R598
primer 5'-
ATAGCCTTAGCTGTAGTGCTGGC
I
I
II
ICCATTCTGTT
-3' (
SEQ
ID NO: 13)
실시예
I-2: 인플루엔자 바이러스 B (
INF
B) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 인플루엔자 바이러스 B의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 인플루엔자 바이러스 B의 서열들을 비교하여 보존서열을 발굴하고 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다. 또한, 분할 구역 이외에도 유전적 다양성을 나타내는 염기 위치에는 I가 오도록 디자인 하였다.
INFB-5'-955 5'-TTGGCTATGACIGAAAGIATAACCIIIIICAGCCCAA-3' (SEQ ID NO: 14)
INFB-3'-1409 5'-TTACATGTTCGGTAAAAITCGTTTAIIIIITCCATACATG-3' (SEQ ID NO: 15)
실시예
I-3: 인간
파라인플루엔자
바이러스 1 (
hPIV
1) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hPIV 1의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hPIV 1의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다.
PIV1-HN-F1 5'-GACCCACATGATTTCTGGAGATGIIIIITAGGAGAACC-3' (SEQ ID NO: 16)
PIV1-HN-R1 5'-CTATTACAGAACATGATTTCCTGTTGTCIIIIITGTCATAGGT-3' (SEQ ID NO: 17)
실시예
I-4: 인간
파라인플루엔자
바이러스 2 (
hPIV
2) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hPIV 2의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hPIV 2의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다.
hPIV-2-5' 5'-TGGACCTCTCCTAAATATICCCAGCIIIIICCCCTCAGCA-3’(SEQ ID NO: 18)
hPIV-2-3' 5'-GTGACTGAACAGCTTTTGCGATTGIIIIIATCACTTAGG-3’ (SEQ ID NO: 19)
실시예
I-5: 인간
파라인플루엔자
바이러스 3 (
hPIV
3) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hPIV 3의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hPIV 3의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다. 또한, 분할 구역 이외에도 유전적 다양성을 나타내는 염기 위치에는 I가 오도록 디자인 하였다.
hPIV-3-5' 5'-TAAGIATAAAATGGACATGGCATAAIIIIITATCAAGACC-3’ (SEQ ID NO:20)
hPIV-3-3' 5'-CGTTTACICTTTCIGTTGCTGTTGAGIIIIITATGACTGGG-3’(SEQ ID NO:21)
실시예
I-6: 인간 호흡기
신시티움
바이러스 A (
hRSV
A) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hRSV A의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hRSV A의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다.
RSVa-F103 5'-AGAATTTTATCAATCAACATGCAGTGIIIIIAGCAAAGGCT-3’ (SEQ ID NO:22)
RSVa-R382 5'-ATTGTTGAGTGTATAATTCATAAACCTTGGIIIIICTCTTCTGGC-3’(SEQ ID NO:23)
실시예
I-7: 인간 호흡기
신시티움
바이러스 B (
hRSV
B) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hRSV B의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hRSV B의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다.
RSVb-F536 5'-TCAGTCTATCAAATGGGGTCAGTGIIIIIACCAGCAAAG-3’ (SEQ ID NO:24)
RSVb-R927 5'-ATTACACCATAGATAGGTAGCTGTACAACIIIIICAAGGACTTC-3’(SEQ ID NO:25)
실시예
I-8: 인간
메타뉴모바이러스
(
hMPV
) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hMPV의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hMPV의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다. 또한, 분할 구역 이외에도 유전적 다양성을 나타내는 염기 위치에는 I가 오도록 디자인 하였다. W은 A 또는 T를 나타낸다.
hMPV-5'-585 5'-AGCTTCAGTCAATTCAACAGAAIIIIICTAAATGTTG-3’ (SEQ ID NO:26)
hMPV-3'-1007 5'-TTGAITGCTCAGCIACATTAATIIIIICWGCTGTGTC-3’ (SEQ ID NO:27)
실시예
I-9: 인간 코로나바이러스
OC43
(
hCoVOC43
) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hCoVOC43의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hCoVOC43의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다.
hCoVOC43-F400 5'-TATGTTAGGCCGATAATTGAGGACTIIIIIACTCTGACGG-3’(SEQ ID NO:28)
hCoVOC43-3' 5'-GTAATTACCGACTTTGGACTTAACATIIIIIGCAAAACCAC-3’(SEQ ID NO:29)
실시예
I-10: 인간 코로나바이러스 229E (
hCoV229E
) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hCoV229E의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hCoV229E의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다. 또한, 분할 구역 이외에도 유전적 다양성을 나타내는 염기 위치에는 I가 오도록 디자인 하였다.
229E-F375 5'-TICTTAAGCAGTATACTTCTGCTTGTIIIIICTATTGAAGA-3’(SEQ ID NO:30)
229E-R375 5'-TGGCCATICGTTCAGCATCAGCGAIIIIIGGCAAAACCA-3’ (SEQ ID NO:31)
실시예
I-11: 인간
리노바이러스
(
hRV
) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 hRV의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 hRV의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머로 이루어진 프라이머 두 세트를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다. 하기의 도 1-2의 결과는 SEQ ID NO:36 과 37을 이용하여 실시한 것이다.
HRV-F151 5'-CCCCACGGGTGACCGTGTCCCAIIIIICGTGGCGGCC-3’ (SEQ ID NO:32)
HRV-R151 5'-GCTCCAGGGTTAAGGTTAGCCGCIIIIIGGGGCCGG-3’ (SEQ ID NO:33)
HRVa-F15 5'-GAAACACGGACACCCAAAGTIIIIIGTCCCRTCCC-3’ (SEQ ID NO:36)
HRV-R353 5'-ATCGTTAICCGCAAAGTGCCTIIIIIAAGCYTAGTA-3’(SEQ ID NO:37)
실시예
I-12:
아데노바이러스
(
Adv
) 핵산 증폭용
프라이머
유전적 다양성을 나타내는 다양한 Adv의 지놈들을 실패 없이 모두 증폭할 수 있는 본 발명의 프라이머를 디자인하기 위하여, 공지된 Adv의 서열들을 비교하여 보존서열을 발굴하고, 전방향 및 역방향 프라이머를 디자인하였다. 유전적 다양성이 나타나는 부분에 DSO의 특징 중 하나인 분할 구역이 위치하도록 디자인하였다. 또한, 분할 구역 이외에도 유전적 다양성을 나타내는 염기 위치에는 I가 오도록 디자인 하였다. R은 A 또는 G를 나타낸다.
HAdV-F1 5'-TCTAIGGCATCTCGATCCAGCAIIIIICCTCGTTTCG-3’ (SEQ ID NO:34)
HAdV-R1 5'-GGACACIIGCTCATGGAIACCARAGIIIIIAAACGCATCAA-3’(SEQ ID NO:35)
실시예
I-13: 종래 형태의
프라이머의
제조
발굴된 서열을 종래 형태의 프라이머 즉, DSO 구조를 가지지 않는 프라이머 형태로 제조하고 대조용 프라이머 세트로 사용하였다.
INFA-F85C 5'-AGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG-3'
INFA-R85C 5'-ATAGCCTTAGCTGTAGTGCTGGCTAAAACCATTCTGTT-3'
INFB-5'-955C 5’-TTGGCTATGACTGAAAGAATAACCAGAGACAGCCCAA-3’
INFB-3'-1409C 5’-TTACATGTTCGGTAAAAGTCGTTTATTCCTTCCATACATG-3’
RSVa-F103C: 5’-AGAATTTTATCAATCAACATGCAGTGCAGTCAGCAAAGGCT-3’
RSVa-R382C: 5’-ATTGTTGAGTGTATAATTCATAAACCTTGGTAGTTCTCTTCTGGC-3’
RSVb-F536C: 5’-TCAGTCTATCAAATGGGGTCAGTGTTTTAACCAGCAAAG-3’
RSVb-R927C: 5’-ATTACACCATAGATAGGTAGCTGTACAACATATGCAAGGACTTC-3’
hCoVOC43-F400C: 5’-TATGTTAGGCCGATAATTGAGGACTACTTTACTCTGACGG-3’
hCoVOC43-3'C: 5’-GTAATTACCGACTTTGGACTTAACATAAACAGCAAAACCAC-3’
실시예
II: 바이러스 시료의 준비
호흡기 바이러스 감염 환자의 코 에스퍼레이트 (nasal aspirate)로부터, RNAzol B 방법을 사용하여 바이러스 총 RNA를 추출하였다 (RNAzol LS; Tel-Test, Inc.).
실시예
III: RT-
PCR
및 멀티플렉스
PCR
실시예
III-1: RT-
PCR
(reverse
transcriptase
-
PCR
)
상기 실시예 II에서 얻은 바이러스 RNA을 사용하여 1.5 시간 동안 37℃에서 반응혼합물 20 ㎕의 역전사 반응을 실시하였다. 상기 반응혼합물 20 ㎕는 5 ㎕의 총 RNA (약 0.5 ㎍), 4 ㎕의 5x 반응 완충액 (Invitrogen, USA), 5 ㎕의 dNTPs (각 2 mM), 2 ㎕의 1 μM 랜덤 헥사디옥시뉴클레오타이드, 0.5 ㎕의 RNase 억제제 (40 units/㎕, Promega), 및 1 ㎕ 몰로니 뮤린 류케미아 바이러스 (M-MLV) 역전사효소 (200 units/㎕, Promega)를 포함한다.
실시예
III-2: 멀티플렉스
PCR
상기 단계에서 얻은 cDNA 산물에 하기의 프라이머 세트를 각각 첨가하여 멀티플렉스 PCR를 실시하였다:
(1) 프라이머 세트 I : AdV, MPV, CoV229E, PIV 1, PIV 2 및 PIV 3 지놈 증폭용 프라이머 쌍;
(2) 프라이머 세트 II : INF A, INF B, RSV A, RSV B, RV 및 CoVOC43 지놈 증폭용 프라이머 쌍.
2 ㎕ (30 ng)의 제 1 가닥 cDNA, 15 mM MgCl2 (Roche)을 함유하는 2 ㎕의 10x PCR 반응 완충액, 2 ㎕의 dNTP (각각 2 mM dATP, dCTP, dGTP 및 dTTP), 4 ㎕의 프라이머 세트 I 또는 II 및 0.5 ㎕의 Taq 중합효소 (5 units/㎕)를 포함하는 최종 20 ㎕의 반응혼합물을 사용하여 멀티플렉스 PCR을 실시하였다.
반응혼합물을 포함하는 튜브를 전가열 (94℃) 된 온도 사이클러 (thermal cycler)에 넣고, 94℃ 15분에서 변성반응시킨 후, 94℃ 30초, 60-65℃ 1.5분 및 72℃ 1.5분의 30-45 사이클 처리하고; 이어 72℃에서 10분 반응시켰다.
PCR 산물을 EtBr을 포함하는 아가로스 겔에 전기영동하고, 나타난 밴드를 용출하고 서열을 확인하였다.
한편, 실시예 I-12에서 제조한 종래 형태의 프라이머 쌍들 (대조용 프라이머 세트)을 상기 프라이머 세트 II 대신 사용하여 동일한 실험을 실시하였다.
실시예
IV: 양성 대조군
PCR
인플루엔자 바이러스 A 및 B; 인간 파라인플루엔자 바이러스 1, 2 및 3; 인간 호흡기 신시티움 바이러스 A 및 B; 인간 메타뉴모바이러스, 인간 코로나바이러스 OC43 및 인간 코로나바이러스 229E; 및 아데노바이러스의 배양액으로부터 얻은 각각의 바이러스 RNA을 사용하여 상기 실시예 III-1과 유사하게 역전사 반응을 수행하였다. 상기 역전사 반응 산물 각각 및 이들의 혼합물을 주형으로 하고 상기 실시예 III-2 단계의 프라이머 세트 I 및 II를 사용하여 PCR을 수행하였다. PCR 산물을 EtBr을 포함하는 아가로스 겔에 전기영동하였다.
본 발명의 프라이머 세트 I 및 II을 이용한 PCR 시에 예상되는 증폭 산물의 크기는 표 2와 같다.
프라이머 세트 I | 증폭 산물 크기 (bp) | 프라이머 세트 II | 증폭 산물 크기(bp) |
AdV | 534 | Inf A | 513 |
MPV | 423 | Inf B | 455 |
CoV229E | 375 | RSV A | 391 |
PIV 1 | 324 | hRV | 337 |
PIV 2 | 264 | RSV B | 273 |
PIV 3 | 219 | CoVOC43 | 231 |
도 1은 양성대조군 PCR 결과를 나타내며, 도 1 에서 알 수 있는 바와 같이, 본 발명의 프라이머는 예상되는 크기의 증폭산물을 제공하였다.
도 2는 24명의 환자로부터 얻은 바이러스 시료에 본 발명의 프라이머 세트를 사용하여 실시한 멀티플렉스 PCR (multiplex PCR) 결과를 나타낸다. 도 2a (프라이머 세트 I) 및 2b(프라이머 세트 II)에서 알 수 있는 바와 같이, 본 발명의 프라이머는 호흡기 바이러스 감염환자가 어떤 바이러스에 감염되어 있는 지를 정확히 검출해 내었다.
도 2c는 프라이머 세트 II 대신 실시예 I-12에서 제조한 종래 형태로 디자인된 프라이머 (대조용 프라이어 세트)를 사용하여 실시한 멀티플렉스 PCR 결과를 나타낸다. 도 3에서 알 수 있는 바와 같이, 종래 형태로 디자인된 프라이머를 사용하는 경우 비-타깃 산물 (non-target product) 및 배경산물 (backgrounds)이 다수 생성되었다.
호흡기 바이러스의 검출에 있어서, 본 발명의 올리고뉴클레오타이드는 PCR 기반 기술의 프라이머로서 뿐만 아니라, 마이크로어레이 기반 기술에서 호흡기 바이러스를 검출하는 프로브로도 유용하게 사용될 수 있다.
본 발명은 타깃 바이러스 핵산에 특이적으로 혼성화되는 올리고뉴클레오타이드, 이를 포함하는 키트 및 이를 사용한 바이러스 검출 또는 증폭 방법을 제공한다. 본 발명의 올리고뉴클레오타이드는 분리 바이러스 (isolates)에 공통적으로 존재하는 보존서열 부위를 기본으로 하여, 이중 특이성 올리고뉴클레오타이드 (dual specificity oligonucleotide; DSO) 형태로 디자인된다. 본 발명의 올리고뉴클레오타이드는 타깃 호흡기 바이러스에 특이적으로 혼성화될 수 있으며, 따라서 호흡기 바이러스 검출에 매우 유용하다.
<110> Seegene, Inc.
<120> Oligonucleotides for Detecting Respiratory Virus Nucleic Acids
<150> KR10-2005-0104023
<151> 2005-11-01
<160> 37
<170> KopatentIn 1.71
<210> 1
<211> 1027
<212> DNA
<213> Influenza virus A
<400> 1
agcgaaagca ggtagatatt gaaagatgag tcttctaacc gaggtcgaaa cgtacgttct 60
ctctatcatc ccgtcaggcc ccctcaaagc cgagatcgca cagagacttg aagatgtctt 120
tgcagggaag aacaccgatc ttgaggttct catggaatgg ctaaagacaa gaccaatcct 180
gtcacctctg actaagggga ttttaggatt tgtgttcacg ctcaccgtgc ccagtgagcg 240
aggactgcag cgtagacgct ttgtccaaaa tgcccttaat gggaacgggg atccaaataa 300
catggacaaa gcagttaaac tgtataggaa gctcaagagg gagataacat tccatggggc 360
caaagaaatc tcactcagtt attctgctgg tgcacttgcc agttgtatgg gcctcatata 420
caacaggatg ggggctgtga ccactgaagt ggcatttggc ctggtatgtg caacctgtga 480
acagattgct gactcccagc atcggtctca taggcaaatg gtgacaacaa ccaacccact 540
aatcagacat gagaacagaa tggttttagc cagcactaca gctaaggcta tggagcaaat 600
ggctggatcg agtgagcaag cagcagaggc catggaggtt gctagtcagg ctaggcaaat 660
ggtgcaagcg atgagaacca ttgggactca tcctagctcc agtgctggtc tgaaaaatga 720
tcttcttgaa aatttgcagg cctatcagaa acgaatgggg gtgcagatgc aacggttcaa 780
gtgatcctct cgctattgcc gcaaatatca ttgggatctt gcacttgata ttgtggattc 840
ttgatcgtct ttttttcaaa tgcatttacc gtcgctttaa atacggactg aaaggagggc 900
cttctacgga aggagtgcca aagtctatga gggaagaata tcgaaaggaa cagcagagtg 960
ctgtggatgc tgacgatggt cattttgtca gcatagagct ggagtaaaaa actaccttgt 1020
ttctact 1027
<210> 2
<211> 2368
<212> DNA
<213> Influenza virus B
<400> 2
agcagaagcg gagctttaag atgaatataa atccatattt tcttttcata gatgtaccta 60
tacaggcagc aatttcaaca acattcccat acaccggtgt tcccccttat tctcatggaa 120
cgggaacagg ctacacaata gacaccgtga ttagaacaca cgagtactca aacaagggaa 180
aacaatacat ttctgatgtt acaggatgtg taatggtaga tccaacaaat gggccattac 240
ccgaagacaa tgaaccgagt gcctatgcac aattggattg tgttctggag gctttggata 300
gaatggatga agaacatcca ggtctgtttc aagcagggtc acagaatgcc atggaggcac 360
taatggtcac aacagtggac aaattgactc aggggagaca gacctttgat tggacggtgt 420
gtagaaacca acctgctgca acggcactga acacaacaat aacctctttt aggttgaatg 480
atttaaatgg agccgacaag ggtggattag tgcccttttg ccaagatatc attgattcat 540
tagacaaacc tgaaatgatt ttcttcacag taaagaatat aaagaaaaaa ttgcctgcta 600
aaaacagaaa gggtttcctt ataaaaagaa tacctatgaa ggtaaaagac agaataacaa 660
gagtggaata catcaaaaga gcattatcat taaacacaat gactaaagat gctgaaagag 720
gcaaactaaa aagaagagca attgccaccg ctgggataca aatcagagga tttgtattag 780
tagttgaaaa cttggctaaa aatatctgtg aaaatctaga gcaaagtggt ttacccgtag 840
gtggaaacga aaagaaggcc aaactatcaa atgcagtggc taaaatgctc agtaattgtc 900
caccaggagg gatcagtatg actgtgacag gagacaatac taaatggaat gaatgcttaa 960
atccaagaat ctttttggct atgactgaaa gaataaccag agacagccca atttggttcc 1020
gggatttttg tagtatagca ccggtcttgt tctccaataa aatagctaga ttgggaaaag 1080
ggttcatgat aacaagtaaa acaaaaagac taaaagctca aataccttgt cccgatctgt 1140
ttaatatacc attagaaaga tataatgaag aaacaagggc aaaactgaaa aagctaaaac 1200
ctttcttcaa tgaagaagga acggcatctc tttcgccagg aatgatgatg ggaatgttta 1260
atatgctatc tacagtatta ggagtagccg cactagggat aaaaaacatt ggaaacaaag 1320
aatacttatg ggatggactg cagtcttcgg atgattttgc tctgtttgtt aatgcaaaag 1380
atgaagagac atgtatggaa ggaataaacg atttttaccg aacatgtaag ctattgggaa 1440
taaacatgag caaaaagaaa agttactgta atgaaactgg gatgtttgaa tttaccagca 1500
tgttttacag agatggattt gtatctaatt ttgcaatgga actcccttca tttggagtcg 1560
ctggagtgaa tgaatcagca gacatggcaa taggaatgac aataataaag aacaatatga 1620
tcaacaatgg gatgggccca gcaacggcac aaacagccat acaattattc atagctgact 1680
atagatacac ctacaaatgc cacaggggag attccaaagt ggaagggaag agaatgaaaa 1740
ttataaagga gctatgggaa aacactaaag gaagagatgg tctattagta gcagatggtg 1800
ggcctaatct ttacaatttg agaaacctgc atattccaga aataatatta aaatacaaca 1860
taatggaccc tgagtacaaa ggacggttac tgcatcctca aaatcccttt gtaggacatt 1920
tgtctattga gggtatcaaa gaagcagata taacacctgc acatggccca ataaagaaaa 1980
tggactacga tgcggtatct ggaactcata gttggagaac caaaaggaac agatctatac 2040
taaacactga tcagaggaac atgattcttg aggaacaatg ctacgctaag tgttgcaacc 2100
tttttgaggc ttgctttaac agtgcgtcat acaggaaacc agtaggccag cacagcatgc 2160
ttgaagctat ggcccacaga ttaagaatgg atgcacgact ggactatgag tcaggaagga 2220
tgtcaaaaga ggatttcgaa aaagcaatgg ctcaccttgg tgagattggg tacatgtaag 2280
ctccggaaat gtctatgggg ttattggtca tcgttgaata catgcggtgc acaaatgatt 2340
aaaatgaaaa aaggctcgtg tttctact 2368
<210> 3
<211> 15600
<212> DNA
<213> Human parainfluenza virus 1
<400> 3
accaaacaag aggaaaaact tgtttggaat atataataat attaaatagt attttagggt 60
taaagtaata ctttaaaggg acaagtcaca gacatttgat cttagtataa atttttataa 120
tggccgggct actaagtact tttgacacat ttagttccag gagaagtgag agcatcaata 180
agtctggcgg aggagcaatt atacctggtc aaagaagtac cgtttctgtc ttcacattag 240
gcccgagtgt gacagatgat gcagataaat tattaatagc aaccactttc ttagcccact 300
cattggacac agataaacaa cactctcaaa gaggaggatt tttagtatca ctccttgcaa 360
tggcctacag tagtccggag ttatatctca ctacaaacgg tgtcaatgct gatgtcaagt 420
atgtgatata caatatagag agagatccta aaagaacaaa aacagatggg ttcattgtca 480
aaacaagaga catggagtat gaaagaacaa cagagtggtt gtttggacct atgatcaaca 540
agaacccatt gttccaaggg caaagagaga atgcggatct agaggcattg cttcagacat 600
atggatatcc tgcatgtctc ggagctataa tagttcaagt ttggatagtg ttggttaaag 660
ccataacaag tagtgctggt ctaagaaaag gattcttcaa tagattagaa gcattcagac 720
aggatggaac cgttaaaagt gccctggtct tcacaggaga cacagttgaa ggtattggtg 780
cagtgatgag gtcacaacaa agcttagtat ctcttatggt agaaactctg gtgactatga 840
acacatccag gtcagattta actacattag agaagaacat tcagattgta ggaaattaca 900
taagagatgc aggattagca tctttcatga acaccatcaa gtatggtgta gaaacgaaga 960
tggccgccct gacactatca aatctgagac cagatataaa caaattgaga agccttgttg 1020
atatctatct atcaaaggga gcccgagccc cttttatatg tatactcaga gacccagttc 1080
atggagactt tgcccctgga aactatccag cactgtggag ctacgcaatg ggcgttgctg 1140
tggtacaaaa caaagctatg caacagtatg taactggaag aacatatttg gacatggaaa 1200
tgttcctact tggacaagct gtagctaaag atgctgattc caaaatcagc agtgctctgg 1260
aggaagaact aggtgtgaca gatacagcaa aagagagact aagacaccat ctgacaaacc 1320
tttcaggagg ggatggtgcg taccacaagc ctacaggtgg tggagctata gaagtggcaa 1380
ttgatcatac agacataaca tttggagtcg aggacactgc tgatcgggac aacaagaact 1440
ggacaaatga cagcaatgaa agatggatga atcactcgat cagcaaccac acaatcacga 1500
ttcgtggtgc agaagaactt gaagaagaga caaatgatga agacatcact gatatagaaa 1560
acaagattgc acgaaggctg gccgacagaa aacagagact aagccaggca aacaataaac 1620
gagacaccag cagtgatgct gactatgaga atgatgatga tgctacagcg gctgcaggga 1680
taggaggaat ttaacaggat acttggacaa tagaagccag atcaaaagta agaaaaactt 1740
agggtgaatg acaattcaca gatcagctca accagacacc accagcatac acgaaaccaa 1800
ccttcacagt ggatacctca gcatccaaaa ctctccttcc cgaatggatc aggatgcctt 1860
cttttttgag agggatcctg aagccgaagg agaggcacca cgaaaacaag aatcactctc 1920
agatgtcatc ggactccttg acgtcgtctt atcctacaag cccacagaaa ttggagaaga 1980
cagaagctgg ctccatggta tcatcgacaa cccaaaagaa aacaagccat catgcaaagc 2040
cgacgataac aacaaagaca gagcaatctc aacgtcgacc caagatcata gatcaagtga 2100
ggggagtgga atctctagga gaacaagtga gtcaaaaaca gagacacatg ctagaatcct 2160
tgatcaacaa ggtatacaca gggcctctag gcgaggaact agtccaaacc ctctacctga 2220
gaatatgggc aatgaaagaa acaccagaat cgacgaagat tctccaaatg agagaagaca 2280
tcagagatca gtacttacgg atgaagacag aaagatggct gagaactcta ataagaggga 2340
agaagaccaa gttgagggat ttccagaaga ggtacgaaga agtacaccct tatctgatga 2400
tggagagggt agaacaaata ataatggaag aagcatggaa actagcagca cacatagtac 2460
aagaataact gatgtcatta ccaacccaag tccagagctt gaagatgccg ttctacaaag 2520
gaacaaaaga cggccgacga ccatcaagcg taaccaaaca agatcagaga gaacacagag 2580
ttcagaactc cacaaatcaa caagtgaaaa tagctccaac ctcgaagacc acaacaccaa 2640
aaccagccca aaagttccac cgtcaaagaa cgaagagtca gcagccactc caaagaacaa 2700
ccacaaccac agaaaaacaa gatacacaac aaacaatgca aacaacaaca caaaaagtcc 2760
accaactccc gaacacgacg caaccgcaaa tgaagaggaa accagcaaca catcggtcga 2820
tgagatggcc aagttattag taagtcttgg tgtaatgaaa tcacaacatg aatttgaatt 2880
atctaggagt gcaagtcatg tatttgctaa gcgcatgtta aaatctgcaa attacaaaga 2940
aatgacattt aatctctgtg gtatgcttat atcagttgaa aaatcacttg agaataaagt 3000
agaagaaaat agaacattac ttaaacaaat tcaagaggaa ataaattcat ccagggatct 3060
tcacaaacgg ttctcggaat accaaaaaga acagaactca ctcatgatgg ccaatctatc 3120
cacactccat ataattacag atagaggcgg gaaaacggga aatcccagtg atactacaag 3180
gtcaccatca gtcttcacaa aagggaaaga caataaggtc aaaaagacaa ggtttgaccc 3240
ctctatggaa gctctaggag gtcaagagtt caagcctgac ttgataagag aggatgaact 3300
gagagatgac atcaaaaatc cggtactaga agaaaacaac aatgagcctc aagcatccaa 3360
tgcatcacgc ctgattccgt ccactgaaaa acacactctg cactcactca aactagttat 3420
cgaaaacagt cctctaagca gagtagagaa gaaggcttac atcaaatccc tttataagtg 3480
tcggacaaac caagaggtta aaaatgtaat ggagctattc gaggaagaca tagattcact 3540
aactaactaa acatgaatct acaatttcaa ccagcaatca aaatcaatat ccagagccaa 3600
ctcaaaaagc tccctcaaaa caattaagaa aaacttaggg tcaaagaaat tttgcccgga 3660
gaaaggaaat ggctgaaaca tacaggttcc ccagattctc acacgaagaa aatgggacag 3720
tagaacctct ccctctcaaa acaggtcctg acaaaaaagc aatccctcac atcagaatag 3780
tcaaggtagg agatcctcca aaacatggag tcaggtatct tgatgtgcta ctattgggat 3840
tctttgaaac acctaagcaa ggacctctat ctggcagcat atctgatctc acagaatcaa 3900
ccagttattc aatctgtgga tccggatcct taccaattgg catagccaag tattacggca 3960
cagatcaaga attattaaaa gcctgcattg acctcaaaat aactgtacga agaacagtta 4020
gatctggaga aatgatagta tacatggtag attcgatcca tgctcctcta ctaccatggt 4080
ccagccgact gagacaaggg atgatatata atgccaataa agtagctcta gcacctcaat 4140
gtctcccagt cgacaaagac atcagattca gggttgtatt tgtcaatgga acatcactag 4200
gtacaattac aattgctaag gtcccaaaaa ctcttgcaga tcttgcatta ccgaactcaa 4260
tatcagtgaa tctgctggtt acacttaggg caggagtatc aacggaacaa aaaggaatcc 4320
tccccgttct agacgatgat ggagaaaaga agctcaactt catggtacac ctaggaatca 4380
taagaagaaa agttgggaag atatattcag ttgaatactg caaaaataaa attgagaaga 4440
tgaagctaat attctctctc gggcttgtag gtggaataag tttccatgta catgcaacag 4500
gcacattatc caaaactcta atgagccaac ttgcatggaa aaaagcagtt tgctatcctt 4560
taatggatgt aaatccacat atgaatctag tcatctgggc agcttcagta gaaatcacaa 4620
gtgtcgatgc tgtgttccaa cctgcaattc cgaaagaatt tcgctattac ccaaatgttg 4680
ttgcaaaaag catcgggaaa atcaggagga tataagtcta cactcctcaa taatgacacc 4740
cattagctct aaatcgtacc attaatcaaa tacagatcaa ttcgatacaa tcagttcaaa 4800
taagaaaaac gtagggacaa agtcctctac caacatcaag gaagacaaga gtctcaaaaa 4860
gctcagccta agcagagaga aaaacaacaa cacaaagaaa gaaaaggaca agatcacaaa 4920
caagaacaaa agcaaaaaca aaaacaagaa caaaaaaggg aagaaaaaca aaagtataca 4980
caaaaaccaa aaaagaaaaa aggccagaga caaaaacgga ggcaagaaca aaaatttaaa 5040
caaaaacaga atttaaattc ataataaaca ccaagataga gacaaaaatg caaaaatcag 5100
agatcctctt cttagtatac tcaagcttgc tattatcttc atcattatgt caaattccgg 5160
tagaaaaact ttcaaatgta ggggttataa tcaatgaggg caaattactt aaaatagcag 5220
gatcttatga atctagatac atagtgttaa gcttggtacc ttcaattgac ctacaagatg 5280
gatgtggaac aactcaaatt attcaataca agaatttatt aaatagactt ctaattcctc 5340
tgaaggatgc cttggatctt caggaatccc tgataacaat aactaatgac accactgtga 5400
caaatgataa tccacaaact agattctttg gtgctgtcat tggtaccata gcactaggag 5460
tagccacagc tgctcaaata actgcgggca ttgcattagc tgaagcacga gaagccagga 5520
aggacatagc actaataaaa gattccatag tcaagacaca caattctgta gaactcattc 5580
aaagaggtat aggagaacag ataattgcat taaagacatt acaagatttt gtaaatgacg 5640
agataagacc tgcaatagga gaactaaggt gtgagactac ggcattgaaa ctagggatca 5700
agctcaccca acactactct gaattagcaa cagcattcag ctccaatctt gggactatag 5760
gagaaaaaag tcttaccttg caggcattat catctctcta ctctgctaat ataacagaaa 5820
ttctaagtac aactaaaaag gataaatcag atatatatga catcatttac actgaacagg 5880
ttaagggaac tgtgatagat gttgatttgg aaaaatacat ggttaccctc ttagttaaaa 5940
taccaatttt atcagaaata ccaggcgtgt tgatatacag agcttcatct atatcttata 6000
atattgaagg agaagaatgg catgtcgcaa tcccaaatta cataatcaat aaggcatcat 6060
ccttaggagg tgcagatgtc acaaactgta tagaatcaaa attggcatat atatgtccta 6120
gagatcctac acaattaata cctgataacc aacagaagtg tatactcggg gatgtatcaa 6180
agtgccctgt gactaaagta ataaacaatc tagtaccaaa gttcgcattc atcaatggtg 6240
gtgtagtggc taattgcatt gcatccacat gtacatgcgg gacaaacaga ataccagtga 6300
atcaagatcg ctcaagagga gttacattct tgacctatac caattgcggt ttaataggta 6360
taaatggaat agaactatat gccaataaaa ggggacgaga cactacttgg gggaatcaaa 6420
tcatcaaagt gggtccagca gtctccatta gacctgtaga catttcttta aatcttgcat 6480
ctgccacaaa tttcctagag gaatccaaga cagagctcat gaaggcaagg gcaatcatat 6540
cagcagttgg aggatggcac aacacagaga gtactcagat aatcatgata ataattgtgt 6600
gcatacttat aataatcata tgtggtatat tatactatct atacagggtt agaagactat 6660
tagtaatgat taattcaact cataattcac ctgttaatgc ttatactctg gagtcaagaa 6720
tgagaaatcc ctacatgggt aacaactcca attaaaaaat cagatcaagt acattgtagc 6780
atacatacaa caatcaaatc tatccacaac ttcaccaatc aggtgtacaa caagtaagaa 6840
aaacttaggg ttaaagacaa tccagtcaac ctataaggca acagcatccg attatacaaa 6900
cgatggctga aaaagggaaa acaaatagtt catattggtc tacaacccga aatgacaatt 6960
ccacggtaaa cacacacatt aatacaccag caggaaggac acacatctgg ctactgattg 7020
caacaacaat gcatacagta ttgtccttca ttatcatgat cctatgcatt gacctaatta 7080
taaaacaaga cacttgtatg aagacaaaca tcatgacagt atcctccatg aacgaaagtg 7140
ccaaaataat caaagagaca atcacagaat taatcagaca agaagtaata tcaaggacca 7200
taaacataca aagttcagta caaagcggga tcccaatatt gttaaacaag caaagcagag 7260
atctcacaca attaatagag aagtcatgca acagacagga attggctcag atatgcgaaa 7320
acaccattgc tattcaccat gcagacggca tatctcctct ggacccacac gatttctgga 7380
gatgtcccgt aggggaaccc ctactgagca acaaccccaa tatctcatta ttacctggac 7440
caagtctact ttctggatcc accacaattt caggatgtgt tagactacct tcattatcaa 7500
ttggtgatgc aatatatgcg tattcatcaa acttaatcac tcaaggatgt gcagatatag 7560
ggaagtcata tcaggtttta caattaggtt acatatcctt aaattcagat atgtatcctg 7620
atttaaaccc ggtaatttct catacctatg acatcaacga caacaggaaa tcatgttctg 7680
taatagctgc aggaacaagg ggttatcagt tatgctcctt gcccactgtg aatgagacta 7740
cagactactc gagtgaaggt atagaagatt tagtatttga catattagat ctcaagggaa 7800
agaccaaatc tcatcgatac aaaaatgaag atataacttt tgaccatcct ttttctgcaa 7860
tgtatccgag tgtaggaagt gggataaaaa ttgaaaatac actcattttc ctagggtacg 7920
gtggcttaac aactccgctc caaggcgaca ctaagtgtgt gataaacaga tgtaccaatg 7980
ttaatcagag tgtttgcaat gatgctctta agataacttg gctaaagaaa agacaagttg 8040
tcaatgtctt aattcgtatc aataattatt tatctgatag gccaaagatt gttgtcgaga 8100
caattccaat aactcaaaat tacttaggtg ccgaaggtag gctacttaaa ctaggtaaaa 8160
agatctacat atatactaga tcttcaggtt ggcactccaa cctgcaaata ggatcattag 8220
atatcaacaa ccccatgacc attaaatggg cgcctcatga agtcctgtct cgaccaggaa 8280
accaagactg caactggtac aacagatgtc cgagagaatg catatcaggt gtatatactg 8340
atgcatatcc actatctcct gatgcagtca atgttgctac aaccacactg tacgcaaaca 8400
catcacgtgt taatcccacc ataatgtact caaatacctc agaaattatc aacatgctaa 8460
gactcaagaa tgtacaacta gaggcagcat acactactac atcatgtatc actcatttcg 8520
ggaagggcta ctgcttccac attgttgaaa tcaaccaagc cagccttaat accttacaac 8580
ctatgttgtt caagacaagt atccctaaaa tatgtaaaat cacatcttga gcagatcaag 8640
acccaacact atatcaatta tgtgaaaacc agatatgatg tataaaaatt taaaaacaaa 8700
gcatgaatag acatttatat gacaaataga ataagaaaaa cttagggtta atgcctgcct 8760
atttgtcaaa tatggataaa caggagtcaa ctcagaattc ctcagacatc ttatatccag 8820
aatgtcactt gaactctccg attgtaaaaa gcaagattgc tcaacttcac gttttgctag 8880
atatcaatca accctatgat ttaaaagata acagtataat aaatatcacc aaatacaaaa 8940
tcagaaatgg aggtttatcg ccccggcaga tcaaaatcag atcgctaggc aaaatcctta 9000
aacaagaaat taaggatatt gatcgttaca cttttgaacc ttatccgatt ttctcattag 9060
agttactcag actggatatc ccagaaatat gtgacaaaat aagatccatt ttttcagtct 9120
ctgatagatt aataagagaa ctatcatctg gatttcaaga attgtggtta aatattctta 9180
gacaattagg ctgtgttgaa gggaaagagg gatttgactc attaaaggat gtagatatca 9240
tcccagatat aactgataaa tataataaaa acacatggta tcgcccattc ttaacatggt 9300
ttagcatcaa atatgatatg agatggatgc aaaagaataa gtcggggaac catttagatg 9360
tctcaaattc tcacaatttt cttgactgta aatcatatat tttgattata tatagagatt 9420
tagtgataat aataaataaa ttaaaattaa ccggttatgt ccttacacct gaattagtat 9480
taatgtattg tgatgttgtc gaaggaagat ggaatatgtc ttcagctgga cgactcgata 9540
aaaggtcatc aaaaataaca tgtaaggggg aagaattatg ggagcttatc gactctttat 9600
ttcccaatct tggtgaggat gtatataata ttatatcact actagaacct ttatcacttg 9660
ctttaataca gttggatgac cctgtaacta atttaaaagg agctttcatg agacatgttt 9720
tgactgagct acatacaatt ttaataaaag ataatatata cacagattca gaagcagaca 9780
gcataatgga atcattgata aagattttca gagagacatc aattgatgaa aaagcagaaa 9840
ttttctcctt ttttagaacg tttggacatc ctagcttaga agcaataact gctgccgata 9900
aagtaaggac acatatgtat tcctccaaaa aaatcatact aaagacacta tatgagtgtc 9960
atgcaatctt ctgtgcaatt ataataaacg gatatagaga aagacacggt ggtcaatggc 10020
cgccatgcga attccccaat catgtatgtc ttgaactcaa gaatgcacaa ggatccaact 10080
ctgcaatttc gtatgaatgt gccgtagaca attatagtag ttttatagga tttaaatttt 10140
taaaatttat tgagcctcaa ttagatgaag atttgacaat ttatatgaag gataaggctc 10200
tatcacctag gaaagcagca tgggattcag tatatcccga cagtaattta tattacaaag 10260
tccctgaatc agaagagact cgtaggttaa tcgaggtttt tataaatgat aataatttta 10320
accctgcgga tattattaat tatgtagagt caggagaatg gttaaatgac gatagcttca 10380
acatatctta cagtctcaaa gaaaaagaaa ttaaacaaga gggtcgactc tttgccaaga 10440
tgacatataa gatgagagca gtccaggtat tagcagaaac actactagca aaaggagtag 10500
gtgagttatt cagtgaaaat gggatggtaa agggagaaat tgacctacta aagagactga 10560
ctacattatc tgtctcaggt gttccaagat ccaactcagt ttacaataat cccatattac 10620
atgagaaatt gatcaaaaat atgaataagt gcaattcaaa tgggtattgg gatgaaagaa 10680
agaaatctaa aaatgaattc aaagctgcag actcatcaac cgaggggtat gagactctga 10740
gctgtttttt aaccaccgat ttgaaaaaat actgtctcaa ctggagattt gaaagtacag 10800
cgttgttcgg tcaaagatgt aatgagatat tcgggtttaa aactttcttt aactggatgc 10860
accctattct agaaaaaagt acaatttatg taggagatcc ttactgtcca gtacctgata 10920
gaatgcacaa agaactccaa gatcatgatg ataccggaat ctttatccat aatccaagag 10980
ggggaataga gggttattgc cagaaattat ggacactaat ctctattagt gcaatccatc 11040
ttgcagctgt taaagttggt gtcagagtgt cagcaatggt acaaggagac aatcaagcta 11100
tagcagtgac atccagagtt cctgtcacac aaacctataa gcaaaaaaag actcacgtct 11160
atgaagaaat cacaagatat ttcggtgcct tgagagaagt tatgtttgat attggacatg 11220
aattaaaatt aaatgagacc attataagta gcaaaatgtt tgtatacagc aaacggatat 11280
attatgatgg gaaaatcctc ccacagtgcc tcaaagcttt aacaagatgt gtattttggt 11340
cagagactct tgtagatgaa aacaggtcag catgctcaaa cattgcaaca tctatagcca 11400
aagctattga gaatggatat tcacctatct taggctattg tattgctctt tttaaaactt 11460
gccaacaggt atgtatatca ttaggaatga ccattaatcc tactattacg tcaactatca 11520
aagatcaata ttttaaaggg aaaaattggt taagatgtgc aatattgatc ccagctaaca 11580
taggagggtt caactatatg tctacagcta gatgttttgt cagaaatata ggtgatccag 11640
cagttgcagc tctagcagac ttaaagagat tcatcaaagc aggtctgtta gataaacagg 11700
tattatatcg tgtgatgaat caagaaccag gagactcaag cttcttagat tgggcatcag 11760
acccttattc atgcaatctc ccacactcac aaagtataac aactataatc aaaaatgtaa 11820
cagctagatc agtattgcag gaatcaccta atcctctcct atcaggtctc ttttcagaat 11880
caagtagtga agaagatctc aacttagcat catttttgat ggataggaaa gccatattgc 11940
ccagagtagc tcacgagatc ttagataact cacttacagg tgtaagagaa gctatagccg 12000
ggatgcttga tacaacgaaa tctctagtaa gagctagtgt caggagagga ggattatcat 12060
atagtatctt aagaagactt ataaattatg atctattaca atatgagacc ttaacaagga 12120
cactcagaaa accggttaag gataatatag aatatgagta tatgtgttca gtagaattgg 12180
caataggatt gaggcaaaaa atgtggtttc atctaactta tggaagacca atccacggtt 12240
tagaaactcc agacccgtta gaattattaa gaggatcatt tattgaaggc tcagaaatat 12300
gtaaattttg tagatcagaa gggaataacc ctatgtatac ttggttctat cttcctgaca 12360
acatcgactt agatacactt agcaatggaa gtcctgccat acgtatccct tattttggtt 12420
ctgctactga tgaaagatca gaggctcaac taggttatgt taagaactta agcaagccgg 12480
caaaagcagc aataagaatc gcaatggttt acacttgggc ttatggaact gatgaaatat 12540
catggatgga agcagcactt atagctcaaa ccagggctaa cttaagttta gagaatttga 12600
agttactcac ccctgtatcg acttctacaa atttgtccca cagattgaga gatactgcta 12660
cacagatgaa attttcaagt gctactttag ttcgagcgag tcgatttatt accatatcta 12720
atgataatat ggcattaaaa gaggcaggag agtctaaaga tactaattta gtttatcaac 12780
aaattatgtt aaccggattg agcttatttg aattcaatat gaggtataaa caaggatcat 12840
tatctaaacc tatgatatta cacttacatt tgaataataa atgctgtatc atagaatctc 12900
ctcaagaatt gaatattcct cctagatcta cattggactt agagatcact caggaaaata 12960
acaagttaat ctatgatcct gatcctctca aggacataga tctagagtta tttagtaagg 13020
ttagggatgt agtacacaca attgatatga attattggtc tgatgatgaa ataattagag 13080
caactagtat atgtacagct atgactattg cagacacaat gtctcaatta gatagagaca 13140
atcttaaaga aatgatagca ctgataaatg atgatgatat aaatagttta atcaccgaat 13200
ttatggttat tgatataccc ttattttgtt ccactttcgg gggtattcta atcaatcaat 13260
ttgcatattc actttacggg ttaaacgtca gagggaggga tgaaatatgg ggatatgtga 13320
tacgcataat taaagacaca tcacatgcag tcctaaaagt actgtccaat gcattatcac 13380
atcctaaaat attcaaacga ttctgggatg caggagttgt agagcctgtt tatggaccta 13440
acttgtccaa tcaagacaag atactgttag ccatttcagt atgtgaatac tctgttgacc 13500
tcttcatgcg tgattggcaa gagggcatac cgcttgaaat atttatttgt gataacgacc 13560
caaatatagc agaaatgaga aaactttcat ttttagctag acatctagca tacttgtgta 13620
gtttggcaga gatagctaaa gagggaccaa aattggaatc tatgacatct ctcgaacgac 13680
tcgaatcatt gaaagagtat ctagaactta cttttttaga cgatcctata ttaagatata 13740
gtcaattgac aggcttagtt attaagatat tcccttcaac gttaacttac atcaggaaat 13800
cttcaattaa ggtgttgaga gtaagaggta tagggatacc agaagtctta gaggactggg 13860
atcctgatgc cgatagtatg ctactagata atataactgc tgaggttcaa cacaatatac 13920
ctttaaagaa gaacgaaaga actcccttct gggggttaag ggtatcaaaa tcacaagttc 13980
tgcgacttag aggttatgaa gagataaaaa gggaagaaag aggaagatca ggtgtaggat 14040
taactctacc ttttgatggg cgatatttat cacaccaatt gagacttttc gggattaata 14100
gcaccagttg tttgaaagca ttggaactta cctatttact gaatcctcta gtcaataagg 14160
ataaagatag attatatctc ggagaaggtg caggtgcaat gctgtcttgt tatgatgcta 14220
cattaggacc ctgcatgaac tattataatt caggtgttaa ttcttgtgat ctcaacggac 14280
aaagagaatt aaatatttat ccttcagaag tggcactggt agggaagaaa ttgaataatg 14340
tcacgagttt atgtcaaaga gttaaggttt tattcaatgg gaatcctgga tcaacttgga 14400
tagggaatga tgaatgtgaa acactaatct ggaatgaatt acagaataat tcaatagggt 14460
ttattcattg tgacatggaa ggtggagaac acaaatgtga tcaggtggtc ttacatgaac 14520
attatagtgt gatcaggatt gcataccttg ttggggataa ggacgttatc ttagtaagca 14580
aaattgcacc aagattaggt acagactgga caaaacaatt aagtttgtat ttaagatact 14640
ggagagatgt cagcttaata gtgttgaaaa catctaaccc agcctctaca gaaatgtatc 14700
tgatatcaaa agatcctaaa tctgatatta tagaggatag taatacagta ttggcaaacc 14760
ttcttccatt atctaaagag gatagtatta agatagaaaa atggattcta gttgagaaag 14820
ccaaagttca tgattggata gttagagaat taaaggaagg gagtgcatcg tcaggtatgc 14880
taagacctta ccatcaagca ttacaaatct tcggatttga gcctaattta aacaaattat 14940
gtagagattt cttatctaca ctaaatatag tagacacaaa aaattgtatt atcacatttg 15000
atagagtatt aagagataca atctttgagt ggactcggat aaaagacgca gataagaagc 15060
taagacttac aggtaaatat gatctatatc ctcttagaga ttcaggtaag ttaaaagtta 15120
tttctagaag gcttgtaata tcttggatag cattgtctat gtctacaaga ctagtaacag 15180
ggtcatttcc agacattaaa tttgaatcaa gactccaatt aggtatagta tcaatatcct 15240
ctcgtgaaat caaaaatctt agggttatat caaagattgt cattgacaaa tttgaagata 15300
ttatacatag tgtgacctat aggttcttga ctaaagaaat aaaaatattg atgaaaattt 15360
tgggagcagt caaattattt ggggcaagac agagcacatc tgctgatatc actaatatcg 15420
atacatcgga ctccatacaa tgatcttata tcttctcatc tttattatct aatttgttta 15480
aagagatgag ttaacaagat aagaaatccc tttaactgac tcataaaaac atagtaagaa 15540
aaacttacaa cagacaagag tattaataat atatcgatat ttcttaaact cttgtctggt 15600
15600
<210> 4
<211> 15646
<212> DNA
<213> Human parainfluenza virus 2
<400> 4
accaagggga gaatcagatg gcatcgttat atgacgaatt gcaaaaagat tacgtaggtc 60
cggaaccact agattcggtg ccggtaacga ttccagtttt atactatctg atcattctct 120
atctctatta aggatatttc tagtctaaag ttcaaaatgt caagtgtttt aaagacattt 180
gaaagattta ctatacaaca ggagcttcag gagcaatctg atgacactcc agtacctctt 240
gagacaatca aacctacaat cagggtattt gtcatcaata ataatgatcc tgtcgtaaga 300
tctagacttt tattctttaa tctacgaatc attatgagta acactgcaag agagggacat 360
agagctggtg ctctcctcag tcttttatca ctaccttctg cagctatgag taatcacatc 420
aaattagcca tgcattcacc agaagccagc atagatagag tagagataac agggtttgag 480
aataattcat tccgagtcat tccagatgct cgatcaacta tgtccagagg agaggtgctg 540
gcttttgaag cattagctga ggacattcct gataccctta atcaccaaac tccatttgta 600
aataatgatg tagaagatga catatttgat gaaacagaga aattcttaga tgtttgctac 660
agtgtgctta tgcaggcatg gatagtaaca tgcaagtgta tgactgctcc tgatcaacca 720
ccagtatcag tagcaaagat ggctaaatat caacaacaag ggagaatcaa tgctaggtat 780
gtactacaac ctgaagcaca aagactaatt cagaatgcca tccgcaagtc aatggtagta 840
aggcatttca tgacttatga gcttcaactt tcacaatcaa gatctttgct agcaaaccgc 900
tactatgcta tggtgggaga cattggcaag tacattgaac acagcggaat gggaggattt 960
ttcttaacac ttaaatatgg acttggaaca agatggccta cattggctct tgcagcattt 1020
tctggggaac tccagaaatt aaaagctctc atgctacatt atcagagtct aggacccatg 1080
gccaagtaca tggctctatt agaatcacca aaactgatgg attttgtccc atctgaatat 1140
ccattagatt atagctatgc aatgggtatt ggaactgtcc ttgatacaaa tatgagaaat 1200
tatgcatacg gtagatcata tttaaatcag caatattttc agctaggagt agaaacagca 1260
aggaaacagc agggagctgt tgacaacagg acagcagagg acctcggcat gactgctgca 1320
gacaaagcag acctcactgc aaccatatca aagctatcct tgtcccaatt acctaggggt 1380
agacaaccaa tatctgaccc atttgctgga gcaaatgaca gagaaatggg aggacaagca 1440
aatgatacac ctgtgtataa cttcaatcca atcgatactc ggaggtatga caactatgac 1500
agtgatggtg aggacagaat tgacaacgat caagatcaag ctatcagaga gaatagagga 1560
gagcctggac aaccaaacaa ccagacaagt gacaaccagc agagattcaa cccccccata 1620
ccgcaaagaa catcaggtat gagcagtgaa gagttccaac attcaatgaa tcagtacatc 1680
cgtgctatgc atgagcaata cagaggctcc caggatgatg atgccaatga tgccacagat 1740
gggaatgaca tttctcttga gctagttgga gattttgatt cctaactctc aatgtcatac 1800
aaccagatat acacatccac atcactcaga gatacagctg ccactcacac actcatccag 1860
acaaatcaaa ctagactcac atcattcgga aacaattctc tcataattta aagaaaaaat 1920
cataggccgg acgggttaga aatccggtgc ttgttcgtga tcagataacc tccacaccag 1980
aatcatacaa tcatggccga ggaaccaaca tacaccactg agcaagttga tgaattaatc 2040
catgctggac tgggaacagt agatttcttc ctatctagac ccatagatgc tcagtcttct 2100
ttaggcaaag gcagcatccc accaggtgtc acagctgttc taactagtgc agcggagaca 2160
aaatccaaac cagttgctgc tggtccagtt aaacccaggc ggaagaaagt gatcagcaat 2220
actactccat acactattgc agacaatatt ccacctgaga agctaccgat caacactcca 2280
atacccaatc cattacttcc actggcacgc cctcacggaa agatgacaga cattgacatt 2340
gtcactggga acattacaga aggatcgtac aaaggtgtgg agcttgctaa attagggaag 2400
cagacactac tcacaaggtt cacctcgaat gagccagtct cctcagctgg atccgcccaa 2460
gaccccaact ttaagagggg gggagctaat agagaaagag caagaggcaa ccataggaga 2520
gaatggagta ttgcatgggt cggagatcag gtcaaagtct tcgagtggtg taatcccagg 2580
tgtgccccag tcacggcctc agctcgcaag ttcacctgca catgcggatc ctgccccagc 2640
atctgcggag aatgtgaagg agatcattga gctcttaaag ggacttgatc ttcgccttca 2700
gactgtagaa gggaaagtag ataaaattct tgcaacttct gcaactataa tcaatcttaa 2760
aaatgaaatg actagtctca aggcgagtgt tgcaactatg gaaggtatga taacaacaat 2820
taaaatcatg gatcccagta caccaactaa tgtccctgta gaggagatca gaaagagttt 2880
acacaatgtt ccagtagtaa ttgccggtcc aactagtgga ggcttcacag ccgaacaggt 2940
gatattgatt tcaatggatg aactagctag acctacactc tcatcaacaa aaaggatcac 3000
acgaaagcct gaatccaaga aagatttaac aggcataaaa ctaactttga tgcagcttgc 3060
aaatgactgc atctcgcgtc cagataccaa gactgagttc gtgactaaga ttcaggcagc 3120
aaccacagaa tcacagctta acgaaattaa acggtcaata atacgctctg caatataaaa 3180
tgaggtgcag tcacacaaga gacactcaac atgcatccaa tcaagatcca gactccatcc 3240
atccaaaaac acgcccacaa ttgtcaacac caagaaacaa ccacagccga accatgctca 3300
accaaaagac ccaaacaaca cctcacatca atagaaggct ggacatgata aatttaataa 3360
aaaaagaaaa gaagttaagt aaaatttaaa ggacacaata gagaaaatct aggtccgaaa 3420
gcttgcctct cagacagatc ccaaaatcat agtccaaacc ccaaacacag cagcagacat 3480
gcctataata tcattaccag cagatccaac ttcacccagt caatccctta ctccgtttcc 3540
aatacaactt gacaccaaag atggcaaggc agggaaactc cttaaacaga ttcgaattag 3600
gtatctaaat gagcctaatt ctcgccatac accaataact ttcatcaata cgtatggatt 3660
tgtttatgct cgagacactt cagggggcat tcacagtgag atcagcagtg acctagctgc 3720
agggtccata acagcatgca tgatgaagct aggacctggt ccaaatattc agaatgcaaa 3780
tctagtgcta agatctctga atgaattcta cgtaaaagtc aagaagacat caagccagag 3840
agaggaagca gtgtttgaat tagttaacat tccaacttta ttgagagaac atgctctttg 3900
caaacgcaaa atgttagtat gctctgcaga aaaattcctc aagaacccgt caaagctaca 3960
agctggattt gagtatgtat acataccaac ttttgtctcc attacatact caccacgaaa 4020
tctgaattac caagtggcca gacctatcct taagttcaga tcacgctttg tgtatagcat 4080
tcatttggaa ttaatcctga gattgctatg caaatctgac tcccccttga tgaaatccta 4140
caatgcagac agaacaggtc ggggatgcct cgcatcagtc tggatccatg tatgtaacat 4200
tctgaaaaac aaaagcatca agcaacaagg cagagaatca tatttcatag ctaagtgcat 4260
gagcatgcag ctgcaggtgt ccattgcaga tctttgggga ccaacaatca taatcaaatc 4320
attgggtcac atccccaaga ctgcacttcc ttttttcagc aaagatggga ttgcctgtca 4380
tccattacaa gatgtttccc ctaatctagc aaaatcactg tggtcagttg gatgtgagat 4440
agaatctgcc aagttgatac ttcaagaatc tgatcttaat gagctaatgg gccaccagga 4500
ccttatcact gataagattg ccattagatc aggtcaacgg acatttgaga ggtccaaatt 4560
cagcccattc aaaaaatatg catcaattcc aaacttggaa gccatcaact gaatgctcca 4620
gcatctgaga atagaaccac aatcaagtca tactactagt cactatacaa taatcaacaa 4680
ttttagtcaa ctgattacca agatgttatc ataggtccga actgatcaat ctaacaaaaa 4740
aactaaacgt tccacaataa atcaacgttc aggccaaaat attcagccat gcatcacctg 4800
catccaatga tagtatgcat ctttgttatg tacactggaa ttgtaggttc agatgccatt 4860
gctggagatc aactacttaa tataggggtc attcaatcaa agataagatc actcatgtac 4920
tatactgatg gtggtgctag ctttattgtt gtaaaattgc tacctaatct tcccccaagc 4980
aatggaacat gcaacatcac cagtctagat gcatataatg ttaccctatt taagttacta 5040
acacccctga ttgagaacct gagtaaaatt tccactgtta cagataccaa aacccgccaa 5100
aaacgatttg caggagtagt tgttggactt gctgcattag gagtagccac agccgcacaa 5160
ataactgcag ctgtagcaat agtgaaagct aatgcaaatg ctgctgcgat aaacaatctt 5220
gcatcttcaa ttcaatccac caacaaggca gtatccgatg tgatagatgc atcaagaaca 5280
attgcaaccg cagttcaagc aattcaggat cgcatcaatg gagctattgt taatgggata 5340
acatctgcat catgccgtgc ccatgatgca ctcattgggt caatattaaa tctttatctc 5400
actgagctta ccacaatatt tcataatcaa ataacaaacc ctgcgctgac accactctcc 5460
atccaagctt taagaatcct cctcggtagc accttgccaa ttgtcattga gtccaaactc 5520
aacacaaact tcaacacagc agagctgctc agttccggac tgttaactgg tcaaataatt 5580
tccatttccc caatgtacat gcaaatgcta attcaaatca atgttccgac atttataatg 5640
caacccggtg cgaaggtaat tgatctaatt gctatctccg caaaccataa attgcaagaa 5700
gtggttgtac aagttccgaa taggattcta gagtatgcaa atgaactaca aaattaccca 5760
gccaatgact gtgtcgtgac accgaactct gtattttgta gatacaatga gggttcccct 5820
atccctgaat cacaatatca atgcttgagg gggaatctta attcttgcac ttttacccct 5880
attatcggga actttcttaa gcgattcgca tttgctaatg gtgtgctcta tgccaactgc 5940
aaatctttgc tatgtaggtg tgccgacccc ccccatgttg tatcccagga tgatacccaa 6000
ggcatcagca taattgatat taagagatgc tctgagatga tgcttgacac tttttcattt 6060
aggatcacat ctactttcaa tgctacgtac gtgacagact tctcaatgat taatgcaaat 6120
attgtacatc taagtcctct agatttgtca aatcaaatca attcaataaa caaatctctt 6180
aaaagtgctg aggattggat tgcagatagc aacttctttg ctaatcaagc caggacagcc 6240
aagacacttt attcactaag tgcaatagca ttaatactat cagtgattac tttggttgtc 6300
gtgggattgc tgattgccta catcatcaag ctggtttctc aaatccatca attcagatcg 6360
ctagctgcta caacaatgtt ccacagggaa aatcctgcct tcttttccaa gaataaccat 6420
ggaaacatat atgggatatc ttaagaaatc tatcacaagt ctatatatgt ccacaattga 6480
cccttaagaa ccaacttcca acgattatcc gttaaattta agtataatag tttaaaaatt 6540
aacattaagc ctccagatac caatgaatat gaatatatct cttagaaaac ctgattatta 6600
tgtgatagcg tagtacaatt taagaaaaaa cctaaaataa gcacgaaccc ttaaggtgtc 6660
gtaacgtctc gtgacaccgg gttcagttca aatatcgacc tctaacccaa tttaacaccc 6720
attcttatat aagaacacag tataatttaa tcacaaaaga cctcaaaaac tgacacagct 6780
tgatccactc aacatataat tgtaagatta ataataatgg aagattacag caatctatct 6840
cttaaatcaa ttcctaaaag gacatgtaga atcattttcc gaactgccac aattcttgga 6900
atatgcacat tgattgttct atgttcaagt attcttcatg agataattca tcttgatgtt 6960
tcctctggtc tcatggattc cgatgattca cagcaaggca ttattcagcc tattatagaa 7020
tcattaaaat cattaattgc tttggctaac cagattctgt acaatgttgc aataataatt 7080
cctcttaaaa ttgacagtat cgagactgta atattctctg ctttaaagga tatgcatact 7140
gggagcatgt ccaacaccaa ctgtacaccc ggaaatctgc ttctgcatga tgcagcgtac 7200
atcaatggaa taaacaaatt ccttgtactt aaatcataca atgggacgcc taaatatgga 7260
cctctcctaa atattcccag ctttatcccc tcagcaacat ctcccaacgg gtgcactaga 7320
ataccatcat tttcactcat taagacccat tggtgttaca ctcacaatgt aatgcttgga 7380
gattgcctcg atttcacgac atctaatcag tatttagcaa tggggataat acaacaatct 7440
gctgcagcat ttccaatctt caggactatg aaaaccattt acctaagtga tggaatcaat 7500
cgcaaaagct gttcagtcac tgctatacca ggaggttgtg tcttgtattg ctatgtagct 7560
acaagatctg agaaagaaga ttatgccaca actgatctag ctgaactgag acttgctttc 7620
tattattata atgatacctt tattgaaaga gtcatatctc ttccaaatac aacagggcaa 7680
tgggccacaa tcaatcctgc agttggaagc gggatctatc atctaggctt tatcttattt 7740
cctgtatatg gtggtctcat aagtgggact ccttcctaca acaagcagtc ctcacgctat 7800
tttatcccaa aacatcccaa cataacctgt gccggtaact ccagcgaaca ggctgcagca 7860
gcacggagtt cctatgtaat ccgttatcac tcaaacaggt tgattcagag tgctgttctt 7920
atttgcccat tgtctgacat gcacacagca aggtgtaatc tagttatgtt taacaattct 7980
caagtcatga tgggtgcaga aggtaggctc tatgttattg acaataattt gtattattat 8040
caacgtagtt cctcttggtg gtctgcatcg cttttttaca ggatcaatac agatttttct 8100
aaaggaattc ctcctatcat tgaggctcaa tgggtaccgt cctatcaagt tccccgtcct 8160
ggagtcatgc catgcaatgc aacaagtttt tgccctgcta attgcatcac aggggtgtac 8220
gcagatgtgt ggccgcttaa cgatccagaa cccacatcac aaaatgctct gaatcccaac 8280
tatcgatttg ctggagcctt tctcagaaat gagtccaacc gaaccaatcc cacattctac 8340
actgcatcag ccagcgccct actaaatact accggattca acaacaccaa tcacaaagca 8400
gcatatacgt cttcaacctg ctttaagaat actggaactc aaaagattta ttgtttgata 8460
ataattgaaa tgggctcatc tcttttaggg gagttccaaa taataccatt tctaagggaa 8520
ctaatacctt aatactattg aatgaagact ccagattcaa taataattga aaggctctct 8580
atcttatgca atagttatac gttttggctg tattagaatg ttatagattc tgctgttttt 8640
cccatatgaa gcaatccttc aacaccgact taggttcaat tttctcatca tttactgttg 8700
taattcaatc ttactaaagt tattccgata tttaagaaaa aataaccttt atataatgta 8760
acaatactat taagattatg atataggcca gaatggcggc ctcttctgag atactccttc 8820
ctgaagtcca cttgaactca ccaatagtca aacacaaact catatactac ttattactag 8880
ggcacttccc gcatgatctt gacatttctg aaataagccc ccttcacaat aatgattggg 8940
atcaaattgc cagagaagaa tccaatcttg ctgaacgact tggagtagct aaatctgaat 9000
taattaaacg tgtgcccgca tttagagcaa ctagatggcg tagtcatgca gccgtcctta 9060
tatggccttc ttgtatacca tttcttgtta aattcctacc tcattctaag cttcaaccag 9120
tagaacaatg gtacaagttg atcaatgctt catgtaatac tatatctgac tcaattgata 9180
gatgtatgga gaatatttct attaagctta ctgggaaaaa caatctattc tctcgatcca 9240
gaggaactgc aggtgcaggt aaaaacagta aaatcaccct caatgatatc caatctattt 9300
gggaatcaaa caagtggcaa cctaatgtat ctttatggct tacaattaaa taccaaatgc 9360
gacaacttat aatgcatcaa agttctcgtc agccgactga tttagttcac attgttgaca 9420
cacgatctgg tctaatagtt atcacccctg aacttgttat ttgttttgat cggttaaata 9480
gtgttttaat gtattttaca tttgagatga ctttaatggt aagtgacatg tttgagggaa 9540
ggatgaatgt caccgctctc tgcactatta gtcattactt atctccacta gggccaagga 9600
tagatagatt gttttccatt gtagatgaat tagcacaact attaggtgac actgtatata 9660
aagttattgc atctcttgaa tctttagtat atgggtgtct acaacttaaa gatccagtag 9720
tggaattagc agggtcattt cattccttta ttacacaaga gattatagat atcctaattg 9780
gttcaaaagc ccttgataag gatgaatcaa taactgttac tacacaattg ttagatatat 9840
tttccaacct ttctccagat ttaattgctg agatgttgtg tctcatgaga ctttggggtc 9900
atcccactct tactgctgcg caagtgggta aagtgagaga atctatgtgt gcaggtaagt 9960
tacttgattt ccctacaata atgaaaactc ttgctttttt ccacacaatt ttaattaatg 10020
gttaccgtag aaagaaaaat ggaatgtggc ctccacttat acttcctaaa aatgcatcaa 10080
aaagcttaat agaatttcaa catgataatg ctgaaatatc ttacgaatat acactcaagc 10140
attggaaaga gatatctctc atagaattta gaaagtgctt tgactttgat cctggtgagg 10200
agctaagcat ttttatgaaa gacaaggcaa taagtgctcc aagaagtgat tggatgagtg 10260
tatttcgtag aagtctaata aaacaacgac atcagagaca tcatattcct atgcccaatc 10320
catttaatag acgtctatta ctcaatttct tagaagatga cagttttgat ccagttgccg 10380
agcttcgata tgttaccggt ggtgaatatc tccaagatga cacattttgt gcatcttact 10440
cattaaaaga gaaagaaata aaaccagatg gaaggatatt tgctaagctt actaatagaa 10500
tgcggtcctg tcaagtaatt gcggaagcaa ttctcgcaaa tcatgcaggt actctaatga 10560
aggaaaacgg agttgtcttg aatcaattat cactgactaa atcattgctt actatgagtc 10620
aaattggcat aatatcagaa aaggcgaaga gatatacgcg agataacatc tcatcccaag 10680
gtttccatac aatcaagact gattctaaaa ataagaggaa aagcaaaact gcatcatcat 10740
acctcacaga tcctgatgat acatttgaac ttagtgcatg ttttataact actgatcttg 10800
ctaaatactg tcttcaatgg agatatcaga ccataatcca ttttgctcga acattaaaca 10860
gaatgtatgg agttccacat ttatttgaat ggattcatct tcgtttaatt agatctacat 10920
tatatgttgg tgatccattc aatcctcctg ccgcaactga tgctttcgat ctagataaag 10980
tattaaatgg tgatatcttt atagtctcca agggaggtat tgaaggccta tgtcagaaaa 11040
tgtggacaat gatctctatt tctgtgatca tcctctcttc agccgaatcc aaaacaagag 11100
taatgagcat ggttcaagga gataatcagg cgattgcagt tacaacaaga gttcctagat 11160
cattacctag tattcagaaa aaggagttag cctatgcagc aagcaagtta ttttttgaaa 11220
gacttagggc aaataattat gggttgggtc atcagctaaa ggctcaagaa actataataa 11280
gttccacgtt cttcatatat agtaaacggg tattttatca aggacgtata ctaacacagg 11340
cactcaaaaa tgctagcaag ttatgtctta ctgcagatgt attaggtgaa tgtactcaag 11400
cttcctgttc aaattctgct actaccatca tgagattaac agaaaatggg gttgagaaag 11460
atacatgtta taagcttaat atttatcagt ccattcgtca actcacatat gatctaatat 11520
ttccccaata ctccatacca ggtgaaacta taagtgagat tttcctacag catccaagac 11580
taatctcacg tattgttctg ctcccttcac agctaggtgg tcttaattac ctcgcatgta 11640
gcagattatt taaccgcaat atcggagatc ctcttggtac agctgtggca gatctcaaga 11700
ggttaattaa atgtggtgct cttgaatcat ggatactgta taatttacta gcaagaaaac 11760
cagggaaagg ttcatgggca actttagcag ccgatccata ctcattgaat caagaatatc 11820
tttatcctcc tactactata cttaaaagac atactcaaaa tactttaatg gagatatgtc 11880
ggaatcctat gttaaaggga gtttttacag ataatgcaaa agaggaggaa aatctccttg 11940
caaaatttct tcttgatcgt gatatagtat tgccaagagt tgcacacatt ataatagatc 12000
aatctagcat cggaaggaag aaacagatac aaggattttt tgacaccaca aggaccataa 12060
tgagacgatc atttgaaatc aaaccactct caactaagaa gactctttca gtcatagaat 12120
ataatactaa ttacttatct tataactacc ctgtcatact taatccttta cctattcctg 12180
gatatttaaa ttatattact gaccaaactt gcagtattga tatatctaga agtttaagaa 12240
aattatcatg gtcttcttta ttgaatggaa gaactttaga aggattagaa actccagatc 12300
caattgaagt tgtcaatggt ttcttgattg taggtacagg agattgtgat ttttgtatgc 12360
agggtgacga caaatttact tggttctttt tacctatggg gataattatt gatggaaatc 12420
ctgaaactaa tccacccatc agagttccat acattgggtc tagaacagag gaaagaagag 12480
ttgcatcaat ggcatatatt aaaggtgcca cacacagttt gaaggctgct cttagaggcg 12540
caggggtata tatttgggca ttcggggata ctgtagtgaa ctggaatgat gcacttgata 12600
tcgcaaatac tagggttaag atatccctag agcaacttca gacccttaca cctcttccta 12660
catctgcaaa cattacacac cgtttagatg atggagccac aacacttaaa ttcactccag 12720
ctagttccta tgcattttct agttatactc atatatcaaa tgatcaacaa tatttagaaa 12780
tagatcagag agtagtcgat tctaatatta tttatcaaca attaatgata acaggacttg 12840
ggattattga gacctaccat aacccaccta taaggacttc tacacaagaa atcactctcc 12900
atttgcacac tagctcatct tgttgtgtta gaagtgtaga tggttgcctt atatgtgaga 12960
gcaatggaga ggttcctcag atcactgttc cctatactaa tacatttgta tatgatcctg 13020
atccactagc agattatgag attgcacacc tagattatct ctcctaccaa gctaaaattg 13080
gaagtacaga ttactactca ctcactgata aaattgacct attagcacat ttaactgcaa 13140
aacaaatgat aaactcaata attgggttag atgaaacagt atcaattgtc aatgatgcgg 13200
ttatcctatc tgactatact aataactgga ttagtgaatg ttcttatact aagatagatt 13260
tagtttttaa attaatggca tggaatttcc ttcttgagct tgcattccag atgtactact 13320
taaggatatc atcttggaca aatatatttg actatactta tatgactttg cgcaggatac 13380
ccggaactgc tctaaataat attgcagcta ctattagcca tccaaaatta ttaagacgtg 13440
caatgaatct tgatattatc actcctatac atgcaccgta tttagcttca ttagattatg 13500
tcaaattaag tattgatgca attcagtggg gagttaaaca agttcttgct gatttatcaa 13560
atggaattga tcttgaaatc ttgattcttt cagaggattc aatggaaatt agtgataggg 13620
caatgaatct cattgctaga aaactaactc tccttgcact tgttaaaggt gagaactata 13680
cttttccaaa aattaaaggg atgccaccag aagaaaagtg tttagtctta actgaatatc 13740
tagcaatgtg ttatcaaaat actcatcact tagatccaga tcttcaaaag tatttatata 13800
atctaactaa tccaaaattg actgcatttc ccagtaacaa cttctactta actagaaaaa 13860
tccttaatca aattagagaa tcagacgaag gacaatatat tatcacctca tattatgaat 13920
ccttcgaaca attagaaaca gatataattc ttcactctac tttaactgct ccttatgata 13980
attcagaaaa ctctaacaaa gttcgattta tccctttcga catctttcca catccagaat 14040
ctctcgagaa atatcctctt ccagttgatc atgactctca atctgcaatt tcaacactaa 14100
ttccaggccc tccttctcat catgtattac gaccactagg agtgtcatcc acagcttggt 14160
ataaagggat aagttattgt agatacctag aaacacaaaa gatacagact ggtgatcatc 14220
tttatttagc cgaaggaagc ggtgcttcaa tgtcacttct agaactctta tttccaggag 14280
atactgtcta ttataatagt ctttttagta gtggagagaa tcctccacag agaaactatg 14340
cccctcttcc aactcaattt gtacagagtg ttccatataa attgtggcaa gctgatcttg 14400
ctgatgatag caatttgata aaagattttg tcccattatg gaatggaaac ggtgcagtta 14460
cagacttatc aacaaaggat gcagttgcat tcataataca taaagtagga gcagagaaag 14520
catcccttgt ccatatagat ctcgaatcaa ctgctaatat aaatcagcaa actctgtcca 14580
gatcccagat tcattcatta attatagcaa ctactgttct taagaggggt gggatattaa 14640
tttataaaac atcatggctt ccgttttcta ggtttagtca actagcaggt ctactttggt 14700
gcttctttga ccggatccat ctaatacgta gtagctattc tgatcctcac agtcatgagg 14760
tttatcttgt atgtagactt gccgcagatt ttagaactat cggtttcagt gcagctctag 14820
taactgctac tactcttcac aatgacggat tcacaacaat acatcctgat gttgtttgta 14880
gttattggca acaccatctt gaaaatgttg ggagagtcgg aaaagtaatt gatgagatac 14940
ttgatggttt agccaccaac ttcttcgcag gagataatgg gcttattcta agatgtggag 15000
gaactccaag ctccagaaaa tggttagaga ttgaccagtt agcatcattt gatttggttc 15060
aagatgctct ggttacactt atcactatac acctaaagga aattatagaa gtgcagtcat 15120
cacatacaga ggattataca tctctcctct tcacacctta taatattggt gcagcaggga 15180
aagtcagaac tatcatcaaa ttaattctag aacgatcttt aatgtataca gtccgaaatt 15240
ggttagtttt acccagttcc atccgggatt ctgtacgaca agatttagaa ttagggtcat 15300
ttagattaat gtctatttta agtgaacaga catttcttaa aaagacaccc acaaaaaaat 15360
acttacttga tcagcttaca aggacatata tatcaacctt ctttaactct cactcagtcc 15420
ttcccctcca ccgtccatat caaaaacaaa tatggaaagc cttaggtagt gtaatatatt 15480
gttcggagac agttgatata cctctaatta aagacattca gatagaagat attaatgatt 15540
ttgaagatat cgagaggggt atcgatggcg aagaattatg acaacaatga ttataagaac 15600
tcatgatagt tttatttaag aaaaacatat tgattttccc cttggt 15646
<210> 5
<211> 15462
<212> DNA
<213> Human parainfluenza virus 3
<400> 5
accaaacaag agaagagact tgtttggaaa tataaattta aattaaaatt aacttaggat 60
taaagacatt gactagaagg tcaagaaaag ggaactctat aatttcaaaa atgttgagcc 120
tatttgatac atttaatgca cgtaggcaag aaaacataac aaaatcagcc ggtggagcta 180
tcattcctgg acagaaaaat actgtctcta tatttgctct tggaccgaca ataactgatg 240
ataatgagaa aatgacatta gctcttcttt ttttatctca ttcactggat aatgagaaac 300
aacatgcaca aagggcaggg ttcctggtgt ccttattatc aatggcttat gccaatccag 360
agctctacct aacaacaaat ggaagtaatg cagatgtcaa gtatgtcata tacatgatcg 420
agaaagatct aaaacggcaa aagtatggag gatttgtggt taagacgaga gagatggtat 480
atgacaagac aactgattgg atatttggaa gtgacctgga ttgtgatcag gaaactatgt 540
tgcagaacgg aagaaacaat tcaacaattg aagaccttgt ccacacattt gggtatccat 600
catgtttagg agctcttata atacagatct ggatagttct ggtcaaagct atcactagta 660
tctcagggtt aagaaaaggc tttttcaccc gattggaagc tttcagacaa gatggaacag 720
tgcaggcagg gctggtattg agcggtgaca cagtggatca gattgggtca attatgcggt 780
ctcaacagag cttggtaact cttatggttg aaacattaat aacaatgaat actagcagaa 840
atgacctcac aaccatagaa aagaatatac aaattgttgg taactatata agagatgcag 900
gtctcgcttc attcttcaat acaatcagat atggaattga gactagaatg gcagctttga 960
ctctatccac tctcagacca gacatcaata gattaaaagc tttgatggaa ctgtatttat 1020
caaagggacc acgcgctcct ttcatctgta tcctcagaga tcccatacat ggtgagttcg 1080
caccaggcaa ctatcctgcc atatggagct atgcaatggg agtggcagtt gtacaaaata 1140
gagccatgca acagtatgtg acgggaagat catatctgga tattgatatg ttccagctag 1200
gacaagcagt agcacgtgat gctgaagctc agatgagctc aacactagaa gatgaacttg 1260
gagtgacaca cgaagctaaa gaaagcttga agagacatat aaggaacata aatagttcag 1320
agacatcttt ccacaaacca acaggtggat cagccataga gatggcaata gatgaagagc 1380
cagaacaatt cgaacataga tcagatcaag aacgagatgg agaacctcaa tcatctataa 1440
ttcaatatgc ttgggcagaa ggaaacagaa gcgatgatcg gactgagcaa gatacagaat 1500
ctgacaatat caagactgaa caacaaaaca tcagagacag actaaacaag agactcaacg 1560
aaaagaagaa acaaggcagt caaccaccta ccaatcccac aaacagaacg aatcaggacg 1620
aaatagatga tctgtttaat gcatttggaa gcaactaatc gaatcaacat tttgatccaa 1680
attaataata aataagaaaa acttaggatt aaagaatcct attataccag aatatagggt 1740
ggtaaattta gagtttgctt gcaactcaat caatagagag ttgatggaaa gcgatgctaa 1800
aaactatcaa atcatggatt cttgggaaga ggaatcaaga gataaatcaa ctaatatctc 1860
ctcggccctc aatatcattg aattcatact tagcaccgac ccccaagaag acctatcgga 1920
aaacgacaca atcaacacaa gaacccagca actcagtgcc accatctatc aaccaaaaat 1980
caaaccaaca gaaacaagtg agaaagatag tggatcaact gacaaaaata gacagtctgg 2040
gtcatcacac gaatgtacaa cagaagcaaa agatagaact attgatcagg aaactgtaca 2100
gagaggacct gggagaagaa gcagctcaga tagtagagct gagactgtgg tctctggagg 2160
aatctccaga agcatcacaa attctaaaaa tggaacccag aacacggagg atattgatct 2220
caatgaaatt agaaagatgg ataaggactc tattgagggg aaagtgcgac aatctgcaga 2280
tgttccaagc gagatatcag gaagtgatgt catatttaca acagaacaaa gtagaaacag 2340
tgatcatgga agaagcttgg aatctatcag tacacctgat acaagatcaa taagtgttgt 2400
tactgctgca acaccagatg atgaagaaga aatactaatg aaaaatagta ggacaaagaa 2460
aagttcttca atacaccaag aagatgacaa aagaattaaa aaagggggaa aagggaaaga 2520
ctggtttaag aaatcaaaag atactgacaa ccagatacca acatcagact acagatccac 2580
atcaaaaggg cagaagaaaa tctcaaaaac aacaaccatc aacaccgaca caaaggggca 2640
aacagaaata cagacagaat catcaggaac acaatcctca tcatggaatc tcaccattga 2700
taacaacaca gatcgaaccg aacagacaaa cacaactccc ccaacaacaa cctccggatc 2760
aacttataca aaagaatcaa tccgaacaaa ctctggatcc aaacccaaga cacaaaagac 2820
aaatggaaag gaaaggaagg atacagaaga gagcaatcga tttacagaga gggcaattac 2880
tctattgcag aatcttggtg taattcaatc cacatcaaaa ctagatttat atcaagacaa 2940
acgagttgta tgtgtagcaa atgtactaaa caatgtagat actgcatcaa agatagactt 3000
cttggcagga ttagtcatag gggtttcaat ggacaacgac acaaaattaa cacagataca 3060
aaatgaaatg ctaaacctca aagcagatct aaagaaaatg gatgaatcac atagaagatt 3120
gatagaaaat caaagagaac aactgtcatt gatcacgtca ttaatttcaa atcttaaaat 3180
tatgactgag agaggaggaa agaaagacca aaatgaatcc aatgagagag tatccatgat 3240
caaaacaaaa ttgaaagaag aaaagatcaa gaaaactagg tttgacccac ttatggagac 3300
acaagggatt gacaagaata tacctgatct atatcgacat gcaggaaata cactagagaa 3360
cgatgtgcaa gttaaatcag agatattaag ttcatacaat gaatcaaatg caacaagact 3420
aatacccaaa aaagtgagca gtacaatgag atcactagtt gcagttataa gcaacagcaa 3480
tctctcacaa agcacaaaac aatcatatat aaacgaactc aaacattgca aaaatgatga 3540
agaagtatct gaattaatgg acatgttcaa tgaagatgtc aataattgcc aatgatccaa 3600
caaagaaacg acaccgaaca aacagacaag aaacaacagt agatcaaaat ctatcaacac 3660
acacaaaatc aagcagaatg aaacaataga tatcaatcaa tatacaaata agaaaaactt 3720
aggattaaag aataaattaa tccttatcca aaatgagtat aactaactct gcaatataca 3780
cattcccaga atcatcattc tttgaaaatg gtcatataga accattacca ctcaaagtca 3840
atgaacagag aaaagcagta ccccacatta gagttgccaa aatcggaaat ccaccaaaac 3900
atggatctcg gtatttagat gtcttcctac taggcttctt cgaaatggaa cgaatcaaag 3960
acaaatacgg gagtgtaaat gatctcgaca gtgacccgag ttacaaagtt tgtggctctg 4020
gatcattacc aatcggattg gctaaataca ctgggaatga ccaggaatta ttacaagctg 4080
caaccaaact ggacatagaa gtgagaagaa cagtcaaagc gaaagagatg gttgtgtata 4140
cagtacaaaa tataaaacca gaactatacc catggtctaa tagactaaga aaaggaatgc 4200
tgttcgatgc caacaaagtt gctcttgctc ctcaatgtct tccactagat aggagcataa 4260
aatttagagt aatcttcgtg aattgtacgg caattggatc aataaccttg ttcaaaattc 4320
ctaagtcaat ggcatcatta tctctaccca acacaatatc aatcaatctg caggtacata 4380
tcaaaacagg ggttcagact gattccaaag ggatagttca aattttgaat gagaagggcg 4440
aaaaatcact gaatttcatg atccatctcg gattgatcaa gagaaaaata ggcagaatgt 4500
actctgttga atactgtaaa cagaaaatcg agaaaatgag attgatattt tctttaggat 4560
tagttggagg aatcagtctt catgtcaatg caactggatc catatcaaaa acactagcaa 4620
gtcagctggt attcaaaagg gagatttgtt atcctttaat ggatctaaat ccgcatctta 4680
atctggttat ctgggcttca tcagtagaga ttacaagagt ggatgcaatt ttccaacctt 4740
ctttacctgg cgaattcaga tactatccta atattatcgc aaaaggagtt gggaaaatca 4800
aacaatggaa ctaataatct ctacttcaat ctggacgcat ccattaagcc gaagcaaata 4860
agggataatc aaaaacttag gacaaaagag gtcaacacca acaactatta gcagtcatac 4920
tcgcaggaac aagaaagaag ggacaaaaaa agcaaaatag gagaaatcaa aacaaaaagt 4980
acagaacacc aaaacaacaa aaccaaaaaa tctgatctat taaaaataaa aatcccaaaa 5040
gagactggca acacagcaaa cactaaacac aatgccaact tcaacactgc taattattac 5100
aaccataatt atggcatctt tctgccaaat agatatcaca aaactacagc atgtaggtgt 5160
attggtcaac agtcccaaag ggatgaagat atcacaaaac ttcgaaacaa gatatttaat 5220
tttgagcctc ataccaaaaa tagaagactc taactcttgt ggtgaccaac agatcaagca 5280
atacaagagg ttattggata gactgatcat ccctctatat gatgggttaa gattacagaa 5340
agatgtgata gtaaccaatc aggaatccaa tgaaaacact aatcccagaa caaaacgatt 5400
ctttggaggg gtaattggaa ccattgctct gggagtagca acctcagcac aaatcacagc 5460
ggcggttgct ctggttgaag ccaagcaggc aagatcagat attgagaaac tcaaagaagc 5520
aatcagggac acaaacaaag cagtgcagtc agttcagagc tccataggga atctaatagt 5580
agcaatcaaa tcagtccaag attatgtcaa caaagaaatc gtgccatcaa ttgcgaggct 5640
aggttgtgaa gcagcaggac ttcaattagg aattgcatta acgcagcatt actcagaatt 5700
aacaaacata tttggtgata acataggatc attacaagaa aaaggaataa aattacaagg 5760
tatagcatca ttataccgca caaatatcac agaaatattc acaacatcaa cagttgataa 5820
atatgatatc tatgatctat tatttacaga atcaataaag gtgagagtta tagatgttga 5880
tttgaatgat tactcaatca ccctccaagt cagactccct ttattaacta ggctgctgaa 5940
cactcagatc tacaaagtag attccatatc atataatatc cacaatagag aatggtatat 6000
tcctcttccc agccatatca tgacgaaagg ggcatttcta ggtggagcag atgtcaaaga 6060
atgtatagaa gcattcagca gttatatatg tccttctgat ccaggatttg tactaaacca 6120
tgaaatggag agctgcttat caggaaacat atcccaatgt ccaagaacca cgatcacatc 6180
agacattgtt ccaagatatg catttgtcaa tggaggagtg gttgcaaact gtataacaac 6240
cacttgtaca tgcaacggaa tcggcaatag aatcaatcaa ccacctaatc aaggagtaaa 6300
aattataaca cataaagaat gtagtacaat aggtatcaac ggaatgctgt tcaatacaaa 6360
caaagaagga actcttgcat tctacacacc agatgatata acattaaaca attctgttgc 6420
acttgatcca attgacatat caatcgagct caacaaggcc aaatcagatc tagaagaatc 6480
aaaagaatgg ataagaaagt caaatcaaaa actagattct attgggaatt ggcatcaatc 6540
tagcaccaca atcataatta ttttgatgat gatcattata ttgtttataa tcaatataac 6600
gataattaca attgcaatta agtattacag aattcaaaag agaaatcaaa tggatcaaaa 6660
tgacaagcca tatgtactaa caaacaaata acatatctat agatcatcag atattaagat 6720
tataaaaaac ttaggagtaa agttacgcaa tccaactcta ctcatacaat tgagaaagaa 6780
cccaatagac aaatccaaat tcgagatgga atactggaag cataccaatc acggaaagga 6840
tgctggtaat gagctggaga cgtccatggc tactcataac aacaagctca ctaataagat 6900
aatatatata ttatggacaa taatcctggt gttattatca atagttttca tcatagtgct 6960
aattaattcc atcaatagtg aaaaggtcca taattcattg ctacaagaaa taaataatga 7020
gtttatggag attacagaaa agatccaaat ggcatcggat aataccaatg atctaataca 7080
gtcaggagtg aacacaaggc ttcttacaat tcagagtcat gtccagaatt atataccaat 7140
atcattgaca caacagatgt cggatcttag gaaattcatt agtgaaatta caattagaaa 7200
tgataatcaa gaagtgccgc aacaaagaat aacacatgat gtaggtataa aacctttaaa 7260
tccagatgat ttttggaggt gcacgtctgg tcttccattt ttaatgagaa acccaaaaat 7320
aaggttaatg ccagggccgg gattattagc catgccaacg actgttgatg gctgtgtcag 7380
aactccgtcc ttaattataa atgatctgat ttatgcttat acctcaaatc taattactcg 7440
aggttgtcag gatataggaa aatcatacca agtcttacag gtagggataa taactgtaaa 7500
ctcagacttg gtacctgact taaatcccag gttctctcat actttcaaca taaatgacaa 7560
taggaagtca tgttctctag cactcctaaa tacagatgta tatcaactgt gttcaactcc 7620
caaagttgat gaaagatcag attatgcatc atcaggcata gaagatattg tacttgatat 7680
tgtcaattat gatggctcaa tctcaacaac aagatttaag aataataaca taagctttga 7740
tcaaccctat gctgcactat acccatctgt tggaccaggg atatattaca aaggcaaaat 7800
aatatttctc gggtatggag gtcttgaaca tccaataaat gagaatgtaa tctgcaacac 7860
aactgagtgt cccgggaaaa cacagagaga ctgcaatcag gcatcttata gtccatggtt 7920
ttcagatagg aggatggtca actccatcat tgttgttgac aaaggcttaa actcaattcc 7980
aaaattaaag gtatggacaa tatctatgag acaaaattac tgggggtcag aaggaaggtt 8040
gattctacta ggtaacaaga tctatatata tacaagatct acaagttggc atagcaaatt 8100
acaattagga ataattgata ttactgatta cagtgatata aggataaaat ggacatggca 8160
taatgtgcta tcaagaccag gaaacgatga atgtccatgg ggacattcat gtccaaatgg 8220
atgtataaca ggagtatata ctgatgcgta tccactaaat cccacaggga gcattgtgtc 8280
atctgtcata ctagactcac agaaatcgag agtgaaccca gtcataactt actcaacagc 8340
aactgaaaga gtaaacgagc tggccatcag aaacagaaca ctctcagctg gatatacaac 8400
aacaagctgc attacacact atgacaaagg atattgtttt catatagtag aaataaatca 8460
gaaaagttca aacacatttc aacctatgtt gttcaaaaca gagattccaa aaagctgcag 8520
tcaatcataa ttaaccataa tatgcattaa cctatctata atacaagtat atgataagta 8580
atcagcaatc agacaatgga caaaaggaaa atataaaaaa cttaggagca aagcgtgctc 8640
ggaaaatgga cactgaatct aacaatggca ctgtatctga catactctat cctgagtgtc 8700
accttaactc tcctatcgtt aaaggtaaaa tagcacaatt acacactatt atgagtctac 8760
ctcagcctta tgatatggat gacgactcaa tactagttat cactagacag aaaataaaac 8820
tcaataaatt agataaaaga caacgatcta ttagaagatt aaaattaata ttaactgaga 8880
aagtgaatga cttaggaaaa tacacattta tcagatatcc agaaatgtca aaagaaatgt 8940
tcaaattata tatacctggt attaacagta aagtgactga attattactt aaagcagata 9000
gaacatatag tcaaatgact gatggattaa gagatctatg gattaacgtg ctatcaaaat 9060
tagcctcaaa aaatgatgga agcaattatg atcttaatga agaaattaat aatatatcaa 9120
aagttcacac aacttataaa tcagataaat ggtataatcc attcaaaaca tggtttacta 9180
tcaagtatga tatgagaaga ttacaaaaag ctcgaaatga gatcactttt aatgttggga 9240
aagattataa cttgttagaa gaccagaaga atttcttatt gatacatcca gaattggttt 9300
tgatattaga taaacaaaac tataatggtt atctaattac tcctgaatta gtactgatgt 9360
attgtgacgt agtcgaaggc cgatggaata taagtgcatg tgctaagcta gatccaaaat 9420
tacaatctat gtatcaaaaa ggtaataacc tatgggaagt gatagataaa ttgtttccaa 9480
ttatgggaga aaagacattt gatgtgatat cattattaga accacttgca ttatccttaa 9540
ttcaaactca tgatcctgtt aaacaactaa gaggagcttt tttaaatcat gtgttatccg 9600
aaatggaatt gatatttgaa tctggagaat cgattaggga atttctgagt gtagattaca 9660
ttgataaaat cttagatatt tttaatgaat ctacaataga tgaaatagca gagattttct 9720
ctttttttag aacatttggg catcctccat tagaggctag tattgcagca gaaaaggtta 9780
gaaaatatat gtatattgag aaacaattaa aatttgacac tgttaataaa tgccatgcta 9840
tcttctgtac aataataatt aacggatata gagagaggca tggtggacag tggcctcctg 9900
tgacattacc tgaccatgca cacgaattca tcataaatgc ttacggttca aactctgcga 9960
tatcatatga aaacgctgtt gattattacc agagcttcat aggaataaaa tttaataaat 10020
tcatagagcc tcagttagat gaggatttga caatttatat gaaagataaa gcattatctc 10080
caaaaaaatc aaattgggac acagtttatc ctgcatctaa tttactgtac cgtactaacg 10140
catccaacga atcacgaaga ttagttgaag tatttatagc agatagtaaa tttgatcctc 10200
atcagatatt ggattatgta gaatctgggg actggttaga tgatccagag tttaatattt 10260
cttatagtct caaagaaaaa gagattaaac aagaaggtag actctttgca aaaatgacat 10320
acaaaatgag agctacacaa gttttatcag agacactact tgcaaataat ataggaaaat 10380
tctttcaaga gaatgggatg gtgaagggag agattgaatt acttaagaga ttaacgacca 10440
tatcaatatc aggagttcca cggtataatg aagtgtacaa taattctaaa agccatacag 10500
atgaccttaa aacctacaat aaaataagta atcttaattt gtcttccaat cagaaatcta 10560
agaaatttga attcaagtca acggatatct acaatgatgg atatgagact gtgagctgtt 10620
tcctaacaac agatctcaaa aaatactgtc ttaattggag atatgaatca acagctctat 10680
ttggagaaac ttgcaaccaa atatttggat taaataaatt gtttaattgg ttacaccctc 10740
gtcttgaagg aagtacaatc tatgtaggtg atccttactg ccctccatca gataaagaac 10800
atatatcatt agaggatcac cctgattctg gtttttatgt tcataaccca agagggggta 10860
tagaaggatt ttgtcaaaaa ttatggacac tcatatctat aagtgcaata catctagcag 10920
ctgttagaat aggcgtgagg gtgactgcaa tggttcaagg agacaatcaa gctatagctg 10980
taaccacaag agtaccaaac aattatgatt acagaattaa gaaggagata gtttataaag 11040
atgtagtgag attttttgat tcattaagag aggtgatgga tgatctaggt catgaactta 11100
aattaaatga aacgattata agtagcaaga tgttcatata tagcaaaaga atctattatg 11160
atgggagaat tcttcctcaa gctctgaaag cattatctag atgtgtcttt tggtcagaga 11220
cagtaataga cgaaacaaga tcagcatctt caaacttggc aacatcattt gcaaaagcaa 11280
ttgagaatgg ttattcacct gttctaggat atgcatgctc aatttttaag aatattcaac 11340
aactatatat tgcccttggg atgaatatca atccaactat aacacagaat atcaaagatc 11400
agtattttaa gaattcaaat tggatgcaat atgcctcttt aatacctgcc agtgttgggg 11460
gattcaatta catggctatg tcaagatgtt ttgtaaggaa tattggtgat ccatcagttg 11520
ctgcattagc tgatattaaa agatttatca aggcgaatct attagaccga agtgttcttt 11580
ataggattat gaaccaagaa ccaggtgagt catctttttt ggactgggct tcagacccat 11640
attcatgcaa tttaccacaa tctcaaaata taaccaccat gataaaaaat ataacagcaa 11700
ggaatgtatt acaagattca ccaaatccat tattatctgg attattcaca aatacaatga 11760
tagaggaaga tgaagaatta gctgagttcc tgatggacag gaaggtaatt ctccctagag 11820
ttgcacatga tattctagat aattctctca caggaattag aaatgctata gctggaatgt 11880
tagatacgac aaaatcatta attcgggttg gcataaatag aggaggactg acatatagtt 11940
tgttgagaaa aatcagtaat tatgatctag tgcaatatga aacactaagt aggactttgc 12000
gattaattgt aagtgataaa atcagatatg aagatatgtg ttcagtagac cttgccatag 12060
cattgcgaca aaagatgtgg attcatttat caggaggaag gatgataagt ggacttgaaa 12120
cacctgaccc attagaatta ttatctgggg tggtaataac aggatcagaa cattgtaaaa 12180
tatgttattc ttcagatggc acaaacccat atacttggat gtatttaccg ggtaatatca 12240
aaatagggtc agcagaaaca ggtgtgtcat cattaagagt tccttatttt ggatcagtca 12300
ctgatgaaag atctgaggca caattaggat atatcaagaa tcttagtaaa cctgcaaaag 12360
ccgcaataag aatagcaatg atatatacat gggcatttgg taatgatgag atatcttgga 12420
tggaagcctc acagatagca caaacacgtg caaattttac actagatagt ctcaaaattt 12480
taacaccggt agctacatca acaaatttat cacacagatt aaaagatact gcaactcaga 12540
tgaaattctc cagtacatca ttaatcagag tcagcagatt cataacaatg tccaatgata 12600
acatgtctat taaagaagct aatgaaacca aagataccaa tcttatttac caacaaataa 12660
tgttaacagg attaagtgtt ttcgaatatt tatttagatt aaaagaaacc acaggacaca 12720
atcctatagt tatgcatctg cacattgaag atgaatgttg tattaaagaa agttttaatg 12780
atgaacatat taatccagag tctacattag aattaattcg atatcctgaa agtaatgaat 12840
ttatttatga taaagaccca ctcaaagatg tggatttgtc aaaacttatg gttattaaag 12900
accattctta tacaattgat atgaattatt gggatgatac tgacatcata catgcaattt 12960
caatatgtac tgcaattaca atagcagata ctatgtcaca attagatcga gataatttaa 13020
aagagataat agtcattgca aatgacgatg atattaatag cttaatcact gaatttttga 13080
ctcttgacat acttgtattt cttaagacat ttggtggatt attagtaaat caatttgcat 13140
acactcttta tagtttaaaa atagaaggta gagatctcat ttgggattat ataatgagaa 13200
cactgagaga tacttcacat tcaatattaa aagtattatc taatgcatta tctcatccta 13260
aagtatttaa gaggttctgg gattgtggag ttttaaaccc tatttatggt cctaatactg 13320
ctagtcaaga ccagataaaa cttgctctct ctatatgtga atatgcacta gatctattta 13380
tgagagaatg gttgaatggt gtatcacttg aaatatacat ttgtgacagt gatatggaag 13440
ttgcaaatga taggaaacaa gcctttattt ctagacatct ttcatttgtt tgttgtttag 13500
cagaaattgc atcttttgga cctaacctgt taaacttaac atacttagag agacttgatc 13560
tattgaaaca atatcttgaa ttaaatatta aagaagaccc tactcttaaa tatgtacaaa 13620
tatctggatt attaattaaa tcgttcccat caactgtaac atacgtaaga aagactgcaa 13680
tcaaatattt aaggattcgc ggtattagtc cacctgaagt aattgatgat tgggatccga 13740
tagaagatga aaatatgctg gataacattg tcaaaactat aaatgataac tgtaataaag 13800
ataataaagg gaataaaatt aacaatttct ggggactagc acttaagaac tatcaagtcc 13860
ttaaaatcag atctataaca agtgattctg atgataatga tagactagat gctagtacaa 13920
gtggtttgac acttcctcaa ggagggaatt atctatcgca tcaattgaga ttattcggaa 13980
tcaacagcac tagttgtttg aaagctcttg agttatcaca aattttaatg aaggaagtta 14040
ataaagacaa ggacaggctc ttcctgggag aaggagcagg agctatgcta gcatgttatg 14100
atgccacatt aggacctgca attaattatt ataattcagg tttgaatata acagatgtaa 14160
ttggtcaacg agaattgaaa atattccctt cagaggtatc attagtaggt aaaaaattag 14220
gaaatgtgac acagattctt aacagggtta aagtactgtt caatgggaat cccaattcaa 14280
catggatagg aaatatggaa tgtgagagct taatatggag tgaattaaat gataagtcta 14340
ttggattagt acattgtgat atggaaggag ctatcggtaa atcagaagaa actgttctac 14400
atgaacatta tagtgttata agaattacat acttgattgg agatgatgat gttgttttag 14460
tttccaaaat tatacctaca atcactccga attggtctag aatactttat ctatataaat 14520
tatattggaa agatgtaagt ataatatcac ttaaaacttc taatcctgca tcaacagaat 14580
tatatctaat ttcgaaagat gcatattgta ctataatgga acctagtgaa gttgttttat 14640
caaaacttaa aagattgtca ctcttggaag aaaataatct attaaaatgg atcattttat 14700
caaagaagag gaataatgaa tggttacatc atgaaatcaa agaaggagaa agagattatg 14760
gagttatgag accatatcat atggcattac aaatctttgg atttcaaatc aacttaaatc 14820
atctggcgaa agaattttta tcaactccag atctgaccaa tatcaacaat ataatccaaa 14880
gttttcagcg aacaatcaaa gatgtcttgt ttgaatggat taatataact catgatgaca 14940
agagacataa attaggcggg agatataaca tattcccact aaaaaataag ggaaagttaa 15000
gactgttatc gagaagacta gtattaagtt ggatttcatt atcattatcg actcgattgc 15060
ttacaggccg ctttcctgat gaaaaatttg aacatagagc acagactgga tatgtatcat 15120
tagctgatac tgatttagaa tcattaaaat tattgtcgaa aaacatcatt aagaattata 15180
gagagtgtat aggatcaata tcatattggt ttctaaccaa agaagttaaa atacttatga 15240
aattgattgg tggtgctaaa ttattaggaa ttcccagaca atataaagaa cccgaagaac 15300
agttattaga aaactacaat caacatgatg aatttgatat cgattaagac ataaatacaa 15360
taaagatata tcttaacctt tatctttgag cccaaggata gacaaaaagt aagaaaaaca 15420
tgtaatatat atataccaag cagagttctt ctcttgtttg gt 15462
<210> 6
<211> 15225
<212> DNA
<213> Human respiratory syncytial virus
<400> 6
acgcgaaaaa atgcgtacta caaacttgca cattcggaaa aaatggggca aataagaatt 60
tgataagtgc tatttaagtc taaccttttc aatcagaaat ggggtgcaat tcactgagca 120
tgataaaggt tagattacaa aatttatttg acaatgacga agtagcattg ttaaaaataa 180
catgttatac tgacaaatta attcttctga ccaatgcatt agccaaagca gcaatacata 240
caattaaatt aaacggtata gtttttatac atgttataac aagcagtgaa gtgtgccctg 300
ataacaacat tgtagtaaaa tctaacttta caacaatgcc aatattacaa aacggaggat 360
acatatggga attgattgag ttgacacact gctctcaatt aaacggtcta atggatgata 420
attgtgaaat caaattttct aaaagactaa gtgactcagt aatgactaat tatatgaatc 480
aaatatctga tttacttggg cttgatctca attcatgaat tatgtttagt ctaactcaat 540
agacatgtgt ttattaccat tttagttaat ataaaaactc atcaaaggga aatggggcaa 600
ataaactcac ctaatcaatc aaactatgag cactacaaat gacaacacta ctatgcaaag 660
attaatgatc acggacatga gacccctgtc gatggattca ataataacat ctctcaccaa 720
agaaatcatc acacacaaat tcatatactt gataaacaat gaatgtattg taagaaaact 780
tgatgaaaga caagctacat ttacattctt agtcaattat gagatgaagc tactgcacaa 840
agtagggagt accaaataca agaaatacac tgaatataat acaaaatatg gcactttccc 900
catgcctata tttatcaatc atggcgggtt tctagaatgt attggcatta agcctacaaa 960
acacactcct ataatataca aatatgacct caacccgtaa attccaacaa aaaaaaccaa 1020
cccaaccaaa ccaagctatt cctcaaacaa caatgctcaa tagttaagaa ggagctaatc 1080
cgttttagta attaaaaata aaagtaaagc caataacata aattggggca aatacaaaga 1140
tggctcttag caaagtcaag ttaaatgata cattaaataa ggatcagctg ctgtcatcca 1200
gcaaatacac tattcaacgt agtacaggag ataatattga cactcccaat tatgatgtgc 1260
aaaaacacct aaacaaacta tgtggtatgc tattaatcac tgaagatgca aatcataaat 1320
tcacaggatt aataggtatg ttatatgcta tgtccaggtt aggaagggaa gacactataa 1380
agatacttaa agatgctgga tatcatgtta aagctaatgg agtagatata acaacatatc 1440
gtcaagatat aaatggaaag gaaatgaaat tcgaagtatt aacattatca agcttgacat 1500
cagaaataca agtcaatatt gagatagaat ctagaaaatc ctacaaaaaa atgctaaaag 1560
agatgggaga agtggctcca gaatataggc atgattctcc agactgtggg atgataatac 1620
tgtgtatagc agcacttgta ataaccaaat tagcagcagg agacagatca ggtcttacag 1680
cagtaattag gagggcaaac aatgtcttaa aaaatgaaat aaaacgctac aagggtctca 1740
taccaaagga tatagctaac agtttttatg aagtgtttga aaaacaccct catcttatag 1800
atgtttttgt gcactttggc attgcacaat catcaacaag agggggtagt agagttgaag 1860
gaatctttgc aggattgttt atgaatgcct atggttcagg gcaagtaatg ctaagatggg 1920
gagttttagc caaatctgta aaaaatatca tgctaggtca tgctagtgtc caggcagaaa 1980
tggagcaagt tgtggaagtc tatgagtatg cacagaagtt gggaggagaa gctggattct 2040
accatatatt gaacaatcca aaagcatcat tgctgtcatt aactcaattt cctaacttct 2100
caagtgtggt cctaggcaat gcagcaggtc taggcataat gggagagtat agaggtacgc 2160
caagaaacca ggatctttat gatgcagcca aagcatatgc agagcaactc aaagaaaatg 2220
gagtaataaa ctacagtgta ttagacttaa cagcagaaga attggaagcc ataaagaatc 2280
aactcaaccc taaagaagat gatgtagagc tttaagttaa caaaaaatac ggggcaaata 2340
agtcaacatg gagaagtttg cacctgaatt tcatggagaa gatgcaaata acaaagctac 2400
caaattccta gaatcaataa agggcaagtt cgcatcatcc aaagatccta agaagaaaga 2460
tagcataata tctgttaact caatagatat agaagtaacc aaagagagcc cgataacatc 2520
tggcaccaac atcatcaatc caacaagtga agccgacagt accccagaaa ccaaagccaa 2580
ctacccaaga aaacccctag taagcttcaa agaagatctc accccaagtg acaacccttt 2640
ttctaagttg tacaaagaaa caatagaaac atttgataac aatgaagaag aatctagcta 2700
ctcatatgaa gagataaatg atcaaacaaa tgacaacatt acagcaagac tagatagaat 2760
tgatgaaaaa ttaagtgaaa tattaggaat gctccataca ttagtagttg caagtgcagg 2820
acccacttca gctcgcgatg gaataagaga tgctatggtt ggtctgagag aagaaatgat 2880
agaaaaaata agagcggaag cattaatgac caatgatagg ttagaggcta tggcaagact 2940
taggaatgag gaaagcgaaa aaatggcaaa agacacctca gatgaagtgc ctcttaatcc 3000
aacttccaaa aaattgagtg acttgttgga agacaacgat agtgacaatg atctgtcact 3060
tgatgatttt tgatcagtga tcaactcact cagcaatcaa caacatcaat aaaacagaca 3120
tcaatccatt gaatcaactg ccagaccgaa caaacaaatg tccgtcagcg gaaccaccaa 3180
ccaatcaatc aaccaactga tccatcagca acctgacgaa attaacaata tagtaacaaa 3240
aaaagaacaa gatggggcaa atatggaaac atacgtgaac aagcttcacg aaggctccac 3300
atacacagca gctgttcagt acaatgttct agaaaaagat gatgatcctg catcactaac 3360
aatatgggtg cctatgttcc agtcatctgt accagcagac ttgctcataa aagaacttgc 3420
aagcatcaac atactagtga agcagatctc tacgcccaaa ggaccttcac tacgagtcac 3480
gattaactca agaagtgctg tgctggctca aatgcctagt aatttcatca taagcgcaaa 3540
tgtatcatta gatgaaagaa gcaaattagc atatgatgta actacacctt gtgaaatcaa 3600
agcatgcagt ctaacatgct taaaagtgaa aagtatgtta actacagtca aagatcttac 3660
catgaagaca ttcaacccca ctcatgagat cattgctcta tgtgaatttg aaaatattat 3720
gacatcaaaa agagtaataa taccaaccta tctaagacca attagtgtca aaaacaagga 3780
tctgaactca ctagaaaaca tagcaaccac cgaattcaaa aatgctatca ccaatgcgaa 3840
aattattccc tatgctggat tagtattagt tatcacagtt actgacaata aaggagcatt 3900
caaatatatc aagccacaga gtcaatttat agtagatctt ggtgcctacc tagaaaaaga 3960
gagcatatat tatgtgacta ctaattggaa gcatacagct acacgttttt caatcaaacc 4020
actagaggat taaatttaat tatcaacact gaatgacagg tccacatata tcctcaaact 4080
acacactata tccaaacatc atgaacatct acactacaca cttcatcaca caaaccaatc 4140
ccactcaaaa tccaaaatca ctaccagcca ctatctgcta gacctagagt gcgaataggt 4200
aaataaaacc aaaatatggg gtaaatagac attagttaga gttcaatcaa tctcaacaac 4260
catttatacc gccaattcaa tacatatact ataaatctta aaatgggaaa tacatccatc 4320
acaatagaat tcacaagcaa attttggccc tattttacac taatacatat gatcttaact 4380
ctaatctctt tactaattat aatcactatt atgattgcaa tactaaataa gctaagtgaa 4440
cataaaacat tctgtaacaa tactcttgaa ctaggacaga tgcatcaaat caacacatag 4500
tgctctacca tcatgctgtg tcaaattata atcctgtata tataaacaaa caaatccaat 4560
cttctcacag agtcatggtg tcgcaaaacc acgccaacta tcatggtagc atagagtagt 4620
tatttaaaaa ttaacataat gatgaattat tagtatggga tcaaaaacaa cattggggca 4680
aatgcaacca tgtccaaaca caagaatcaa cgcactgcca ggactctaga aaagacctgg 4740
gatactctca atcatctaat tgtaatatcc tcttgtttat acagattaaa tttaaaatct 4800
atagcacaaa tagcactatc agttctggca atgataatct caacctctct cataattgca 4860
gccataatat tcatcatctc tgccaatcac aaagttacac taacaacggt cacagttcaa 4920
acaataaaaa accacactga aaaaaacatc accacctacc ttactcaagt cccaccagaa 4980
agggttagct catccaaaca acctacaacc acatcaccaa tccacacaaa ttcagccaca 5040
acatcaccca acacaaagtc agaaacacac cacacaacag cacaaaccaa aggcagaacc 5100
accacctcaa cacagaccaa caagccgagc acaaaaccac gcctaaaaaa tccaccaaaa 5160
aaaccaaaag atgattacca ttttgaagtg ttcaacttcg ttccctgtag tatatgtggc 5220
aacaatcaac tttgcaaatc catctgtaaa acaataccaa gcaacaaacc aaagaagaaa 5280
ccaaccatca aacccacaaa caaaccaacc accaaaacca caaacaaaag agacccaaaa 5340
acaccagcca aaacgacgaa aaaagaaact accaccaacc caacaaaaaa accaaccctc 5400
acgaccacag aaagagacac cagcacctca caatccactg tgctcgacac aaccacatta 5460
gaacacacaa tccaacagca atccctccac tcaaccaccc ccgaaaacac acccaactcc 5520
acacaaacac ccacagcatc cgagccctct acatcaaatt ccacccaaaa tacccaatca 5580
catgcttagt tattcaaaaa ctacatctta gcagaaaacc gtgacctatc aagcaagaac 5640
gaaattaaac ctggggcaaa taaccatgga gctgctgatc cacaggttaa gtgcaatctt 5700
cctaactctt gctattaatg cattgtacct cacctcaagt cagaacataa ctgaggagtt 5760
ttaccaatcg acatgtagtg cagttagcag aggttatttt agtgctttaa gaacaggttg 5820
gtataccagt gtcataacaa tagaattaag taatataaaa gaaaccaaat gcaatggaac 5880
tgacactaaa gtaaaactta taaaacaaga attagataag tataagaatg cagtgacaga 5940
attacagcta cttatgcaaa acacaccagc tgccaacaac cgggccagaa gagaagcacc 6000
acagtatatg aactatacaa tcaataccac taaaaaccta aatgtatcaa taagcaagaa 6060
gaggaaacga agatttctgg gcttcttgtt aggtgtagga tctgcaatag caagtggtat 6120
agctgtatcc aaagttctac accttgaagg agaagtgaac aagatcaaaa atgctttgtt 6180
atctacaaac aaagctgtag tcagtctatc aaatggggtc agtgttttaa ccagcaaagt 6240
gttagatctc aagaattaca taaataacca attattaccc atagtaaatc aacagagctg 6300
tcgcatctcc aacattgaaa cagttataga attccagcag aagaacagca gattgttgga 6360
aatcaacaga gaattcagtg tcaatgcagg tgtaacaaca cctttaagca cttacatgtt 6420
aacaaacagt gagttactat cattgatcaa tgatatgcct ataacaaatg atcagaaaaa 6480
attaatgtca agcaatgttc agatagtaag gcaacaaagt tattctatca tgtctataat 6540
aaaggaagaa gtccttgcat atgttgtaca gctacctatc tatggtgtaa tagatacacc 6600
ttgctggaaa ttacacacat cacctctatg caccaccaac atcaaagaag gatcaaatat 6660
ttgtttaaca aggactgata gaggatggta ttgtgataat gcaggatcag tatccttctt 6720
tccacaggct gacacttgta aagtacagtc caatcgagta ttttgtgaca ctatgaacag 6780
tttgacatta ccaagtgaag tcagcctttg taacactgac atattcaatt ccaagtatga 6840
ctgcaaaatt atgacatcaa aaacagacat aagcagctca gtaattactt ctcttggagc 6900
tatagtgtca tgctatggta aaactaaatg cactgcatcc aacaaaaatc gtgggattat 6960
aaagacattt tctaatggtt gtgactatgt gtcaaacaaa ggagtagata ctgtgtcagt 7020
gggcaacact ttatactatg taaacaagct ggaaggcaag aacctttatg taaaagggga 7080
acctataata aattactatg accctctagt gtttccttct gatgagtttg atgcatcaat 7140
atctcaagtc aatgaaaaaa tcaatcaaag tttagctttt attcgtagat ctgatgaatt 7200
actacataat gtaaatactg gcaaatctac tacaaatatt atgataacta caattattat 7260
agtaatcatt gtagtattgt tatcattaat agctattggt ttgctgttgt attgcaaagc 7320
caaaaacaca ccagttacac taagcaaaga ccaactaagt ggaatcaata atattgcatt 7380
cagcaaatag acaaaaaacc acctgatcat gtttcaacaa cagtctgctg atcaccaatc 7440
ccaaatcaac ccataacaaa cacttcaaca tcacagtaca ggctgaatca tttcttcaca 7500
tcatgctacc cacacaacta agctagatcc ttaactcata gttacataaa aacctcaagt 7560
atcacaatca aacactaaat caacacatca ttcacaaaat taacagctgg ggcaaatatg 7620
tcgcgaagaa atccttgtaa atttgagatt agaggtcatt gcttgaatgg tagaagatgt 7680
cactacagtc ataattactt tgaatggcct cctcatgcct tactagtgag gcaaaacttc 7740
atgttaaaca agatactcaa gtcaatggac aaaagcatag acactttgtc tgaaataagt 7800
ggagctgctg aactggacag aacagaagaa tatgctcttg gtatagttgg agtgctagag 7860
agttacatag gatctataaa caacataaca aaacaatcag catgtgttgc tatgagtaaa 7920
cttcttattg agatcaatag tgatgacatt aaaaagctga gagataatga agaacccaat 7980
tcacctaaga taagagtgta caatactgtt atatcataca ttgagagcaa tagaaaaaac 8040
aacaagcaaa caatccatct gctcaaaaga ctaccagcag acgtgctgaa gaagacaata 8100
aaaaacacat tagatatcca caaaagcata atcataagca acccaaaaga gtcaaccgtg 8160
aatgatcaaa atgaccaaac caaaaataat gatattaccg gataaatatc cttgtagtat 8220
atcatccata ttgatttcaa gtgaaagcat gattgctaca ttcaatcata aaaacatatt 8280
acaatttaac cataaccatt tggataacca ccagcgttta ttaaataata tatttgatga 8340
aattcattgg acacctaaaa acttattaga tgccactcaa caatttctcc aacatcttaa 8400
catccctgaa gatatatata caatatatat attagtgtca taatgcttgg ccataacgat 8460
tctatatcat ccaaccataa aactatctta ataaggttat gggacaaaat ggatcccatt 8520
attaatggaa actctgctaa tgtgtatcta actgatagtt atttaaaagg tgttatctct 8580
ttttcagaat gtaatgcttt agggagttac ctttttaacg gcccttatct caaaaatgat 8640
tacaccaact taattagtag acaaagtcca ctactagagc atatgaatct taaaaaacta 8700
actataacac agtcattaat atctagatat cataaaggtg aactgaaatt agaagaacca 8760
acttatttcc agtcattact tatgacatat aaaagcatgt cctcgtctga acaaattgct 8820
acaactaact tacttaaaaa aataatacga agagctatag aaataagtga tgtaaaggtg 8880
tacgccatct tgaataaact aggactaaag gaaaaggaca gagttaagcc caacaataat 8940
tcaggtgatg aaaactcagt acttacaact ataattaaag atgatatact ttcggctgtg 9000
gaaagcaatc aatcatatac aaattcagac aaaaatcact cagtaaatca aaatatcact 9060
atcaaaacaa cactcttgaa aaaattgatg tgttcaatgc aacatcctcc atcatggtta 9120
atacactggt tcaatttata tacaaaatta aataacatat taacacaata tcgatcaaat 9180
gaggtaaaaa gtcatgggtt tatattaata gataatcaaa ctttaagtgg ttttcagttt 9240
attttaaatc aatatggttg tatcgtttat cataaaggac tcaaaaaaat cacaactact 9300
acttacaatc aatttttaac atggaaagac atcagcctta gcagattaaa tgtttgctta 9360
attacttgga taagtaattg tttgaataca ttaaataaaa gcttagggct gagatgtgga 9420
ttcaataatg ttgtgttatc acaattattt ctttatggag attgtatact gaaattattt 9480
cataatgaag gcttctacat aataaaagaa gtagagggat ttattatgtc tttaattcta 9540
aacataacag aagaagatca atttaggaaa cgattttata atagcatgct aaataacatc 9600
acagatgcag ctattaaggc tcaaaagaac ctactatcaa gggtatgtca cactttatta 9660
gacaagacag tgtctgataa tatcataaat ggtaaatgga taatcctatt aagtaaattt 9720
cttaaattga ttaagcttgc aggtgataat aatctcaata atttgagtga gctatatttt 9780
ctcttcagaa tctttggaca tccaatggtt gatgaaagac aagcaatgga tgctgtaaga 9840
attaactgta atgaaactaa gttctactta ttaagtagtc taagtacgtt aagaggtgct 9900
ttcatttata gaatcataaa agggtttgta aatacctaca acagatggcc cactttaagg 9960
aatgctattg tcctacctct aagatggtta aactattata aacttaatac ttatccatct 10020
ctacttgaaa tcacagaaaa tgatttgatt attttatcag gattgcggtt ctatcgtgaa 10080
tttcatctgc ctaaaaaagt ggatcttgaa atgataataa atgacaaagc catttcacct 10140
ccaaaagatc taatatggac tagttttcct agaaattaca tgccatcaca tatacaaaat 10200
tatatagaac atgaaaagtt gaagttctct gaaagcgaca gatcaagaag agtactagag 10260
tattacttga gagataataa attcaatgaa tgcgatctat acaattgtgt agtcaatcaa 10320
agctatctca acaactctaa tcacgtggta tcactaactg gtaaagaaag agagctcagt 10380
gtaggtagaa tgtttgctat gcaaccaggt atgtttaggc aaatccaaat cttagcagag 10440
aaaatgatag ccgaaaatat tttacaattc ttccctgaga gtttgacaag atatggtgat 10500
ctagagcttc aaaagatatt agaattaaaa gcaggaataa gcaacaagtc aaatcgttat 10560
aatgataact acaacaatta tatcagtaaa tgttctatca ttacagatct tagcaaattc 10620
aatcaagcat ttagatatga aacatcatgt atctgcagtg atgtattaga tgaactgcat 10680
ggagtacaat ctctgttctc ttggttgcat ttaacaatac ctcttgtcac aataatatgt 10740
acatatagac atgcacctcc tttcataaag gatcatgttg ttaatcttaa tgaagttgat 10800
gaacaaagtg gattatacag atatcatatg ggtggtattg agggctggtg tcaaaaactg 10860
tggaccattg aagctatatc attattagat ctaatatctc tcaaagggaa attctctatc 10920
acagctctga taaatggtga taatcagtca attgatataa gtaaaccagt tagacttata 10980
gagggtcaga cccatgctca agcagattat ttgttagcat taaatagcct taaattgcta 11040
tataaagagt atgcaggtat aggccataag cttaagggaa cagagaccta tatatcccga 11100
gatatgcagt tcatgagcaa aacaatccag cacaatggag tgtactatcc agccagtatc 11160
aaaaaagtcc tgagagtagg tccatggata aatacaatac ttgatgattt taaagttagt 11220
ttagaatcta taggtagctt aacacaggag ttagaataca gaggggaaag cttattatgc 11280
agtttaatat ttaggaacat ttggttatac aatcaaattg ctttgcaact ccgaaatcat 11340
gcattatgta acaataagct atatttagat atattgaaag tattaaaaca cttaaaaact 11400
ttttttaatc ttgatagtat cgatatggcg ttatcattgt atatgaattt gcctatgctg 11460
tttggtggtg gtgatcctaa tttgttatat cgaagctttt ataggagaac tccagacttc 11520
cttacagaag ctatagtaca ttcagtgttt gtgttgagct attatactgg tcacgattta 11580
caagataagc tccaggatct tccagatgat agactgaaca aattcttgac atgtgtcatc 11640
acattcgata aaaatcccaa tgccgagttt gtaacattga tgagggatcc acaggcgtta 11700
gggtctgaaa ggcaagctaa aattactagt gagattaata gattagcagt aacagaagtc 11760
ttaagtatag ctccaaacaa aatattttct aaaagtgcac aacattatac taccactgag 11820
attgatctaa atgacattat gcaaaatata gaaccaactt accctcatgg attaagagtt 11880
gtttatgaaa gtctaccttt ttataaagca gaaaaaatag ttaatcttat atcaggaaca 11940
aaatccataa ctaatatact tgaaaaaaca tcagcaatag atacaactga tattaatagg 12000
gctactgata tgatgaggaa aaatataact ttacttataa ggatacttcc actagattgt 12060
aacaaagaca aaagagagtt attaagttta gaaaatctta gtataactga attaagcaag 12120
tatgtaagag aaagatcttg gtcattatcc aatatagtag gagtaacatc gccaagtatt 12180
atgttcacaa tggacattaa atatacaact agcactatag ccagtggtat aattatagaa 12240
aaatataatg ttaatagttt aactcgtggt gaaagaggac ctactaagcc atgggtaggt 12300
tcatctacgc aggagaaaaa aacaatgcca gtgtacaata gacaagtttt aaccaaaaag 12360
caaagagacc aaatagattt attagcaaaa ttagactggg tatatgcatc catagacaac 12420
aaagatgaat tcatggaaga actgagtact ggaacacttg gactgtcata tgaaaaagcc 12480
aaaaagttgt ttccacaata tctaagtgtc aattatttac accgtttaac agtcagtagt 12540
agaccatgtg aattccctgc atcaatacca gcttatagaa caacaaatta tcatttcgat 12600
actagtccta tcaatcatgt attaacagaa aagtatggag atgaagatat cgacattgtg 12660
tttcaaaatt gcataagttt tggtcttagc ctgatgtcgg ttgtggaaca attcacaaac 12720
atatgtccta atagaattat tctcataccg aagctgaatg agatacattt gatgaaacct 12780
cctatattta caggagatgt tgatatcatc aagttgaagc aagtgataca aaaacagcat 12840
atgttcctac cagataaaat aagtttaacc caatatgtag aattattcct aagtaacaaa 12900
gcacttaaat ctggatctaa catcaattct aatttaatat tagtacataa aatgtctgat 12960
tattttcata atgcttatat tttaagtact aatttagctg gacattggat tctaattatt 13020
caacttatga aagattcaaa aggtattttt gaaaaagatt ggggagaggg gtacataact 13080
gatcatatgt tcattaattt gaatgttttc tttaatgctt ataagactta tttgctatgt 13140
tttcataaag gttatggtaa agcaaaatta gaatgtgata tgaacacttc agatcttctt 13200
tgtgttttgg agttaataga cagtagctac tggaaatcta tgtctaaagt tttcctagaa 13260
caaaaagtca taaaatacat agtcaatcaa gacacaagtt tgcatagaat aaaaggctgt 13320
cacagtttta agttgtggtt tttaaaacgc cttaataatg ctaaatttac cgtatgccct 13380
tgggttgtta acatagatta tcacccaaca catatgaaag ctatattatc ttacatagat 13440
ttagttagaa tggggttaat aaatgtagat aaattaacca ttaaaaataa aaacaaattc 13500
aatgatgaat tttacacatc aaatctcttt tacattagtt ataacttttc agacaacact 13560
catttgctaa caaaacaaat aagaattgct aattcagaat tagaagataa ttataacaaa 13620
ctatatcacc caaccccaga aactttagaa aatatatcat taattcctgt taaaagtaat 13680
aatagtaaca aacctaaatt ttgtataagt ggaaataccg aatctataat gatgtcaaca 13740
ttctctaata aaatgcatat taaatcttcc actgttacca caagattcaa ttatagcaaa 13800
caagacttgt acaatttatt tccaaatgtt gtgatagaca ggattataga tcattcaggt 13860
aatacagcaa aatctaacca actttacatc accacttcac atcagacatc tttagtaagg 13920
aatagtgcat cactttattg catgcttcct tggcatcatg tcaatagatt taactttgta 13980
tttagttcca caggatgcaa gatcagtata gagtatattt taaaagatct taagattaag 14040
gaccccagtt gtatagcatt cataggtgaa ggagctggta acttattatt acgtacggta 14100
gtagaacttc atccagacat aagatacatt tacagaagtt taaaagattg caatgatcat 14160
agtttaccta ttgaatttct aagattatac aacgggcata taaacataga ttatggtgag 14220
aatttaacca ttcctgctac agatgcaact aataacattc attggtctta tttacatata 14280
aaatttgcag aacctattag catctttgtc tgcgatgctg aattacctgt tacagccaat 14340
tggagtaaaa ttataattga atggagtaag catgtaagaa agtgcaagta ctgttcttct 14400
gtaaatagat gcattttaat cgcaaaatat catgctcaag atgatattga tttcaaatta 14460
gataacatta ctatattaaa aacttacgtg tgcctaggta gcaagttaaa aggatctgaa 14520
gtttacttag tccttacaat aggccctgca aatatacttc ctgtttttga tgttgtgcaa 14580
aatgctaaat tgattttttc aagaactaaa aatttcatta tgcctaaaaa aactgacaag 14640
gaatctatcg atgcaaatat taaaagctta atacctttcc tttgttaccc tataacaaaa 14700
aaaggaatta agacttcatt gtcaaaattg aagagtgtag ttaatgggga tatattatca 14760
tattctatag ctggacgtaa tgaagtattc agcaacaagc ttataaacca caagcatatg 14820
aatatcctaa aatggctaga tcatgtttta aattttagat cagctgaact taattacaat 14880
catttataca tgatagagtc cacatatcct tacttaagtg aattgttaaa tagtttaaca 14940
accaatgagc tcaagaaact gattaaaata acaggtagtg tactatacaa ccttcccaac 15000
gaacagtaac ttaaaatatc attaacaagt ttggtcaaat ttagatgcta acacatcatt 15060
atattatagt tattaaaaaa tatgcaaact tttcaataat ttagcttact gattccaaaa 15120
ttatcatttt atttttaagg ggttgaataa aagtctaaaa ctaacaatga tacatgtgca 15180
tttacaacac aacgagacat tagtttttga cacttttttt ctcgt 15225
<210> 7
<211> 13335
<212> DNA
<213> Human metapneumovirus
<400> 7
acgcgaaaaa aacgcgtata aattaagtta caaaaaaaca tgggacaagt gaaaatgtct 60
cttcaaggga ttcacctgag tgatctatca tacaagcatg ctatattaaa agagtctcag 120
tatacaataa agagagatgt aggcacaaca acagcagtga caccctcatc attgcaacaa 180
gaaataacac tattgtgtgg agaaattcta tatgctaagc atgctgatta caaatatgct 240
gcagaaatag gaatacaata tattagcaca gctctaggat cagagagagt acagcagatt 300
ctaagaaact caggcagtga agtccaagtg gttttaacca gaacgtactc cttggggaaa 360
gttaaaaaca acaaaggaga agatttacag atgttagaca tacacggagt agagaaaagc 420
tgggtggaag agatagacaa agaagcaaga aaaacaatgg caactttgct taaagaatca 480
tcaggcaata ttccacaaaa tcagaggcct tcagcaccag acacacctat aatcttatta 540
tgtgtaggtg ccttaatatt taccaaacta gcatcaacta tagaagtggg attagagacc 600
acagtcagaa gagctaaccg tgtactaagt gatgcactca aaagataccc taggatggac 660
ataccaaaaa tcgctagatc tttctatgat ttatttgaac aaaaagtgta ttacagaagt 720
ttgttcattg agtatggcaa agcattaggc tcatcctcta caggcagcaa agcagaaagt 780
ttattcgtta atatattcat gcaagcttac ggtgctggtc aaacaatgct gaggtgggga 840
gtcattgcca ggtcatctaa caatataatg ttaggacatg tatctgtcca agctgagtta 900
aaacaagtca cagaagtcta tgacctggtg cgagaaatgg gccctgaatc tgggctccta 960
catttaaggc aaagcccaaa agctggactg ttatcactag ccaattgtcc caactttgca 1020
agtgttgttc tcggcaatgc ctcaggctta ggcataatag gtatgtatcg cgggagagtg 1080
ccaaacacag aactattttc agcagcagaa agctatgcca agagtttgaa agaaagcaat 1140
aaaattaact tttcttcatt aggactcaca gatgaagaaa aagaggctgc agaacacttt 1200
ctaaatgtga gtgacgacag tcaaaatgat tatgagtaat taaaaaagtg ggacaagtca 1260
aaatgtcatt ccctgaagga aaagatattc ttttcatggg taatgaagcg gcaaaattgg 1320
cagaagcttt ccaaaaatca ttaagaaaac ctagtcataa aagatctcaa tctattatag 1380
gagaaaaagt gaacactgta tctgaaacat tggaattacc tactatcagt agacctacca 1440
aaccgaccat attgtcagag ccgaagttag catggacaga caaaggtggg gcaatcaaaa 1500
ctgaagcaaa gcaaacaatc aaagttatgg atcctattga agaagaagag tttactgaga 1560
aaagggtgct gccctccagt gatgggaaaa ctcctgcaga aaagaagttg aaaccatcaa 1620
ccaatactaa aaagaaggtc tcatttacac caaatgaacc aggaaaatac acaaagttgg 1680
agaaagatgc tctagacttg ctttcagaca atgaagaaga agatgcagaa tcctcaatct 1740
taaccttcga agaaagagat acttcatcat taagcattga agccagacta gaatcgattg 1800
aggagaaatt aagcatgata ttagggctat taagaacact caacattgct acagcaggac 1860
ccacagcagc aagagatggg atcagagatg caatgattgg cataagggag gaactaatag 1920
cagacataat aaaagaagcc aagggaaaag cagcagaaat gatggaagaa gaaatgaacc 1980
agcggacaaa aataggaaac ggtagtgtaa aattaactga aaaggcaaag gagctcaaca 2040
aaattgttga agacgagagc acaagtggtg aatccgaaga agaagaagaa ctaaaagaca 2100
cacaggaaaa taatcaagaa gatgacattt accagttaat tatgtagttt aataaaaata 2160
aaaaatggga caagtgaaaa tggagtccta tctggtagac acctatcaag gcatccctta 2220
cacagcagct gttcaagttg atctagtaga aaaggacctg ttacctgcaa gcctaacaat 2280
atggttcccc ctgtttcagg ccaatacacc accagcagtt ctgcttgatc agctaaagac 2340
tctgactata actactctgt atgctgcatc acaaagtggt ccaatactaa aagtgaatgc 2400
atcggcccag ggtgcagcaa tgtctgtact tcccaaaaag tttgaagtca atgcgactgt 2460
agcacttgac gaatatagca aattagaatt tgacaaactt acagtctgtg aagtaaaaac 2520
agtttactta acaaccatga aaccatatgg gatggtatca aagtttgtga gctcggccaa 2580
accagttggc aaaaaaacac atgatctaat cgcattatgc gattttatgg atctagaaaa 2640
gaacacacca gttacaatac cagcatttat caaatcagtt tctatcaagg agagtgaatc 2700
agccactgtt gaagctgcaa taagcagtga agcagaccaa gctctaacac aagccaaaat 2760
tgcaccttat gcgggactga tcatgattat gaccatgaac aatcccaaag gcatattcaa 2820
gaagcttgga gctgggaccc aagttatagt agaactagga gcatatgtcc aggctgaaag 2880
cataagtaaa atatgcaaga cttggagcca tcaaggaaca agatatgtgc tgaagtccag 2940
ataacagcca agcaacctga ccaagaacta ccaactctat tctatagact aaaaagtcgc 3000
cattttagtt atataaaaat caagttagaa taagaattaa atcaatcaag aacgggacaa 3060
ataaaaatgt cttggaaagt ggtgatcatt ttttcattgc taataacacc tcaacacggt 3120
cttaaagaga gctacctaga agaatcatgt agcactataa ctgagggata tcttagtgtt 3180
ctgaggacag gttggtatac caacgttttt acattagagg tgggtgatgt agaaaacctt 3240
acatgttctg atggacctag cctaataaaa acagaattag atctgaccaa aagtgcacta 3300
agagagctca aaacagtctc tgctgaccaa ttggcaagag aggaacaaat tgagaatccc 3360
agacaatcta ggtttgttct aggagcaata gcactcggtg ttgcaacagc agctgcagtc 3420
acagcaggtg ttgcaattgc caaaaccatc cggcttgaga gtgaagtcac agcaattaag 3480
aatgccctca aaacgaccaa tgaagcagta tctacattgg ggaatggagt tcgagtgttg 3540
gcaactgcag tgagagagct gaaagacttt gtgagcaaga atttaactcg tgcaatcaac 3600
aaaaacaagt gcgacattga tgacctaaaa atggccgtta gcttcagtca attcaacaga 3660
aggtttctaa atgttgtgcg gcaattttca gacaatgctg gaataacacc agcaatatct 3720
ttggacttaa tgacagatgc tgaactagcc agggccgttt ctaacatgcc gacatctgca 3780
ggacaaataa aattgatgtt ggagaaccgt gcgatggtgc gaagaaaggg gttcggaatc 3840
ctgatagggg tctacgggag ctccgtaatt tacatggtgc agctgccaat ctttggcgtt 3900
atagacacgc cttgctggat agtaaaagca gccccttctt gttccggaaa aaagggaaac 3960
tatgcttgcc tcttaagaga agaccaaggg tggtattgtc agaatgcagg gtcaactgtt 4020
tactacccaa atgagaaaga ctgtgaaaca agaggagacc atgtcttttg cgacacagca 4080
gcgggaatta atgttgctga gcaatcaaag gagtgcaaca tcaacatatc cactacaaat 4140
tacccatgca aagtcagcac aggaagacat cctatcagta tggttgcact gtctcctctt 4200
ggggctctgg ttgcttgcta caaaggagta agctgttcca ttggcagcaa cagagtaggg 4260
atcatcaagc agctgaacaa gggttgctcc tatataacca accaagatgc agacacagtg 4320
acaatagaca acactgtata tcagctaagc aaagttgagg gtgaacagca tgttataaaa 4380
ggcagaccag tgtcaagcag ctttgatcca atcaagtttc ctgaagatca attcaatgtt 4440
gcacttgacc aagtttttga gaacattgaa aacagccagg ccttggtaga tcaatcaaac 4500
agaatcctaa gcagtgcaga gaaagggaat actggcttca tcattgtaat aattctaatt 4560
gctgtccttg gctctagcat gatcctagtg agcatcttca ttataatcaa gaaaacaaag 4620
aaaccaacgg gagcacctcc agagctgagt ggtgtcacaa acaatggctt cataccacac 4680
agttagttaa ttaaaaataa aataaaattt gggacaaatc ataatgtctc gcaaggctcc 4740
atgcaaatat gaagtgcggg gcaaatgcaa cagaggaagt gagtgtaagt ttaaccacaa 4800
ttactggagt tggccagata gatacttatt aataagatca aactatctat taaatcagct 4860
tttaaggaac actgatagag ctgatggcct atcaataata tcaggcgcag gcagagaaga 4920
cagaacgcaa gattttgttc taggttccac caatgtggtt caaggttata ttgatgataa 4980
ccaaagcata acaaaagctg cagcctgcta cagtctacac aacataatca agcaactaca 5040
agaagttgaa gttaggcagg ctagagatag caaactatct gacagcaagc atgtggcact 5100
ccataactta atcttatctt acatggagat gagcaaaact cccgcatctt taatcaacaa 5160
tctcaaaaga ctgccgagag aaaaactgaa aaaattagca aagctgataa ttgacttatc 5220
agcaggcgct gacaatgact cttcatatgc cctgcaagac agtgaaagca ttaatcaagt 5280
gcagtgagca tggtcctgtt ttcattacta tagaggttga tgaaatgata tggactcaaa 5340
aagaattaaa agaagctttg tccgatggga tagtgaagtc tcacaccaac atttacaatt 5400
gttatttaga aaacatagaa attatatatg tcaaggctta cttaagttag taaaaacaca 5460
tcagagtggg ataagtgaca atgataacat tagatgtcat taaaagtgat gggtcttcaa 5520
aaacatgtac tcacctcaaa aaaataatca aagaccattc tggtaaagtg cttattgcac 5580
ttaagttaat attagcttta ctaacatttt tcacaataac aatcactata aattacataa 5640
aagtagaaaa caatctacaa atatgccagt caaaaactga atcagacaaa gaagactcac 5700
catcaaatac cacatccgtc acaaccaaga ccactctaga ccatgatata acacagtatt 5760
ttaaaagatt aattcaaagg tatacagatt ctgtaataaa caaggacaca tgctggaaaa 5820
taagcagaaa tcaatgcaca aatataacaa catataaatt tttatgcttt aaacctgagg 5880
actcaaaaat caacagttgt gatagactga cagatctatg cagaaacaaa tcaaaatcag 5940
cagctgaagc atatcataca gtagaatgcc attgcatata cacaattgag tggaagtgct 6000
atcaccactc aatagattaa acccaatctt gaatgttaaa actagactag gatccgtcta 6060
agactatcag ttcaatagtt tagttatttt aaaatatttg agaataggta agtttctatg 6120
gcacttcata gcaataggta ataattaaca gcttaattat aattaaaaca ttatttaaaa 6180
tcgtaactat ttaatttaca aagtaaaaac aaaaatatgg gacaagtagt tatggaggtg 6240
aaagtagaga acattcgagc aatagacatg ctcaaagcaa gagtgaaaaa tcgtgtggca 6300
cgtagcaaat gctttaaaaa tgcttcttta atcctcatag gaataactac actgagtata 6360
gctctcaata tctatctgat cataaactac acaatacaaa aaacctcatc tgaatcagaa 6420
caccacacca gctcaccacc cacagaatcc aacaaggaag cttcaacaat ctccacagac 6480
aacccagaca tcaatccaaa ctcacagcat ccaactcaac agtccacaga aaaccccaca 6540
ctcaaccccg cagcatcagt gagcccatca gaaacagaac cagcatcaac accagacaca 6600
acaaaccgcc tgtcctccgt agacaggtcc acagcacaac caagtgaaag cagaacaaag 6660
acaaaaccga cagtccacac aagaaacaac ccaagcacag cttccagtac acaatcccca 6720
ccacgggcaa caacgaaggc aatccgcaga gccaccactt tccgcatgag cagcacagga 6780
aaaagaccaa ccacaacatc agtccagtcc gacagcagca ccacaaccca aaatcatgaa 6840
gaaacaggtt cagcgaaccc acaggcatct gtaagcacaa tgcaaaacta gcacaccaac 6900
aatataaaac caaattagtt aacaaaaaat acgagatagc tctaaagtaa aacatgtagg 6960
taccaacaat caagaaacca aaagacaact cacaatctcc ctaaaacagc aacgacacca 7020
tgtcagcttt gctcaaatct ctctgggaga aacttttgcc cacatactaa caacatcaca 7080
accatctcaa gaaaagaaac tgggcaaaac agcatccaag agacaaatag caatggatcc 7140
tcttaatgaa tccactgtta atgtctatct ccctgattcg taccttaaag gagtaatttc 7200
ttttagtgaa actaatgcaa ttggttcatg tctcttaaaa agaccctact taaaaaatga 7260
caacactgca aaagttgcca tagagaatcc tgttattgag catgtgagac tcaaaaatgc 7320
agtcaattct aaaatgaaaa tatcagatta caaggtagta gagccagtaa acatgcaaca 7380
tgaaataatg aagaatgtac acagttgtga gctcacacta ttgaaacagt ttttaacaag 7440
gagtaaaaac attagcactc tcaaattgaa tatgatatgt gattggctgc aattaaagtc 7500
tacatcagat gatacctcaa tcctaagttt catagatgta gaatttatac ctagttgggt 7560
aagcaactgg tttagtaatt ggtacaatct caataagtta attttggaat tcagaagaga 7620
ggaagtaata agaaccggtt caatcttatg caggtcattg ggtaaattag tttttattgt 7680
atcatcatat ggatgtatcg tcaagagcaa caaaagcaaa agagtgagct tcttcacata 7740
caatcaactg ttaacatgga aagatgtgat gttaagtaga tttaatgcga atttttgtat 7800
atgggtaagc aatagtctga atgaaaatca ggaagggcta gggttgagaa gtaatctgca 7860
aggtatgtta actaataaac tatatgaaac tgtagattat atgctaagct tatgttgcaa 7920
tgaaggtttc tcacttgtga aagagttcga aggttttatt atgagtgaga tccttaggat 7980
tactgaacat gctcaattca gtactagatt tagaaatact ttattgaatg gattaacaga 8040
tcaattaaca aaattaaaaa ataaaaacag actcagagtt catagtaccg tattagaaaa 8100
taatgattat ccaatgtatg aagttgtact taaattatta ggagatactt tgagatgtat 8160
caaattatta atcaataaaa acttagagaa tgctgcagaa ttatactata tattcagaat 8220
ttttggtcat ccaatggtag atgaaagaga tgcaatggat gctgtcaaat taaacaatga 8280
aatcacaaaa atcctaaggt tggagagctt gacagaacta agaggggcat tcatattaag 8340
gattatcaaa ggatttgtgg acaacaacaa aaggtggccc aaaattaaaa acttaaaagt 8400
gcttagcaaa agatggacta tgtacttcaa agctaaaaat taccccagtc aactcgaatt 8460
aagtgaacaa gactttctag agcttgctgc aatacaattt gaacaagagt tttctgttcc 8520
tgaaaaaacc aatcttgaga tggtattaaa tgacaaagcc atatcacctc ctaaaagatt 8580
aatatggtct gtgtatccaa agaattactt acctgagacg ataaaaaatc gatatttaga 8640
agaaactttc aatgcgagtg atagtctcaa aacaagaaga gtactagagt actatttaaa 8700
agataataaa tttgatcaaa aggaacttaa aagttatgta gttagacaag aatatttaaa 8760
cgataaggag cacattgtct cattaactgg aaaagaaaga gaattaagtg taggtagaat 8820
gtttgctatg caaccaggaa aacagcgaca aatacaaata ttggcagaaa aattgttagc 8880
tgataacatt gtacctttct tcccggaaac cttaacaaag tatggtgatc tagatcttca 8940
gagaataatg gaaatcaaat cagaactttc ttctatcaaa accagaagaa atgatagtta 9000
taataattac attgcaagag catccatagt aacagatttg agcaagttca accaagcctt 9060
tagatatgaa actacagcga tctgtgcgga tgtagcagac gaattacatg gaacacaaag 9120
cttattctgt tggttacatc ttatcgttcc tatgactaca atgatatgtg cctatagaca 9180
tgcaccacca gaaacaaaag gtgaatatga tatagataag atagaagagc aaagtggtct 9240
atatagatat cacatgggcg gtattgaagg atggtgtcaa aaactctgga caatggaagc 9300
tatatcttta ttggatgttg tatctgtaaa gacacggtgt caaatgacat ctttattaaa 9360
cggtgacaac caatcaatag atgtaagtaa accagtcaag ttatctgaag gtttagatga 9420
agtgaaggca gattatcgct tagcagtaaa aatgctaaaa gaaataagag atgcatacag 9480
aaatataggc cataaactta aagaagggga aacatatata tcaagggatc ttcagtttat 9540
aagcaaggtg attcaatctg aaggagtgat gcatcctacc cctataaaaa aggtcttgag 9600
agtaggacca tggataaaca caatattaga tgacattaaa actagtgctg agtcaatagg 9660
gagtctatgt caagaattag aatttagggg agaaagcata atagttagtc tgatattaag 9720
aaacttctgg ctgtataact tatacatgca tgaatcaaag caacatcctt tggcagggaa 9780
acagttattc aaacaactaa ataaaacatt aacatcagtg cagagatttt ttgaaattaa 9840
aagggaaaat gaggtagtag atctatggat gaacatacca atgcaatttg gaggaggaga 9900
tccagtagtc ttctatagat ctttctatag aaggacccct gattttttaa ctgaggcaat 9960
cagccatgta gatattctgt taaaaatatc agctaacata aaaaatgaaa cgaaagtaag 10020
tttcttcaaa gccttactat caatagaaaa aaatgaacgt gctacactga caacactaat 10080
gagagatcct caagctgttg gatcagaacg acaagcaaaa gtaacaagtg acatcaatag 10140
aacagcagtt accagtatct taagtctttc cccaaatcaa cttttcagtg atagtgctat 10200
acactacagc agaaatgaag aagaagtggg aatcattgca gaaaacataa cacctgttta 10260
tcctcatggg ctgagagtat tatatgaatc attgcccttt cacaaagctg aaaaagttgt 10320
aaacatgata tcagggacaa aatctataac caacttatta cagagaacat ccgctattaa 10380
tggtgaagat attgacaggg ctgtatctat gatgttggag aatctaggat tattatctag 10440
aatattgtca gtagttgttg atagtataga aattccaatc aaatctaatg gtaggctgat 10500
atgttgtcaa atctctagga ctttaagaga gacatcatgg aataatatgg aaatagttgg 10560
agtaacatct cctagcatca ctacatgtat ggatgtcata tatgcaacta gttctcattt 10620
gaaagggata attatagaaa agttcagcac tgacagaact acaaggggtc aaagaggtcc 10680
aaaaagccct tgggtagggt cgagtactca agagaaaaaa ttagtacctg tttataacag 10740
acaaattctc tcaaaacaac aaagagaaca gctagaagca attggaaaaa tgagatgggt 10800
gtataaaggg acaccaggct tgcgacgatt actcaacaag atctgtcttg ggagtttagg 10860
cattagctac aaatgtgtaa aacctttatt acctaggttt atgagtgtaa atttcttaca 10920
tagattatct gtcagtagta gacctatgga attcccagca tcagttccag cttatagaac 10980
aacaaattac catttcgaca ctagtcctat taatcaagca ctaagtgaga gatttgggaa 11040
tgaagatatt aacttggtct tccaaaatgc aatcagctgt ggaattagca taatgagtgt 11100
agtagaacaa ttaacaggta gaagcccaaa acagttagtt ttaatacccc aattagaaga 11160
aatagacatt atgccaccac cagtgtttca agggaaattc aattataaat tagtagataa 11220
gataacttct gatcaacata tctttagtcc ggacaaaata gatatgttaa cactagggaa 11280
aatgctcatg cccactataa aaggtcagaa aacagatcag ttcttaaata agagagaaaa 11340
ttatttccat ggaaacaatc ttattgagtc tttatcagca gcattagcat gtcattggtg 11400
tgggatatta acagaacaat gcatagaaaa taatattttc aagaaagact ggggtgacgg 11460
gtttatatca gatcatgctt ttatggactt caaaatattc ctatgtgtct ttaaaactaa 11520
acttttatgt agttggggat ctcaagggaa aaacattaaa gatgaagata tagtagatga 11580
atcaatagat aaattgttaa ggattgacaa tactttttgg agaatgttca gcaaagttat 11640
gtttgaacca aaggttaaga aaaggataat gttatatgat gtaaaattcc tatcactagt 11700
aggctacata gggtttaaga actggtttat agagcagttg agatcagctg aattgcatga 11760
aataccttgg attgtcaatg ccgaaggtga tttggttgag atcaagtcaa ttaaaatcta 11820
tttgcaactg atagaacaaa gcttattttt aagaataact gttttgaact atacagatat 11880
ggcacatgct ctcacacgat taatcagaaa gaagttaatg tgtgataatg cactgttaac 11940
cccaatttca tccccaatgg ttaacttaac tcaagttatt gatcccacaa cacaattaga 12000
ttacttcccc aagataacat tcgaaaggct aaaaaattat gacacaagtt caaattatgc 12060
taaagggaag ctaacaagaa attacatgat actattgcca tggcagcatg ttaatagata 12120
taactttgtc tttagttcta ctggatgtaa agttagtctg aaaacatgta ttggaaaact 12180
tatgaaagac ctaaatccta aagttttgta ctttattgga gaaggagcag gaaattggat 12240
ggccagaaca gcatgtgaat atcctgatat taaatttgta tatagaagtc tgaaagatga 12300
ccttgatcat cattatcctc tggaatacca gagagtgata ggtgaattaa gcagaatcat 12360
agatagtggt gaaggacttt caatggaaac aacagacgca actcaaaaaa ctcattggga 12420
tttgatacac agggtaagca aagatgcttt attaataact ttatgtgatg cagaatttaa 12480
ggacagagat gattttttta agatggtaat tctatggaga aaacatgtat tatcatgcag 12540
aatttgcact acttatggga cggacctcta tttattcgca aagtatcatg ctaaagactg 12600
caatgtaaaa ttaccttttt ttgtgagatc agttgctact ttcattatgc agggtagtaa 12660
gctgtcaggt tcagaatgct acatactctt aacactaggc caccacaaca gtttaccttg 12720
ccatggagaa atacaaaatt ctaagatgaa aatagcagtg tgtaatgatt tttatgctgc 12780
aaaaaaactc gacaataaat caattgaagc taattgtaaa tcacttttgt cagggctaag 12840
aatacctata aataagaagg aactagatag acagagaaga ttattaacac tacaaagcaa 12900
tcattcttct gtagcaacag ttggcggtag caagatcata gagtctaagt ggttaacaaa 12960
caaagcaagt acaataattg attggttaga acatatttta aattctccaa agggcgaatt 13020
aaattatgat ttttttgaag cattggagaa cacttaccct aatatgatta aactaataga 13080
taacttaggg aatgcagaga ttaaaaaact gatcaaagta acaggataca tgcttgtaag 13140
taaaaaatga aaaatgatga agatgacaaa atagatgaca acttcatact attctaaatt 13200
aattatttga ttatgcaatt atatgatagt taattaaaat taaaaattaa aaatcaaaag 13260
ttaaaattta aaacctatca ttaagtttat taaaaataag aaattataat tgaatgtata 13320
cggttttttt gccgt 13335
<210> 8
<211> 30738
<212> DNA
<213> Human coronavirus OC43
<400> 8
gattgtgagc gatttgcgtg cgtgcatccc gcttcactga tctcttgtta gatctttttg 60
taatctaaac tttataaaaa catccactcc ctgtaatcta tgcttgtggg cgtagatttt 120
tcatagtggt gtttatattc atttctgctg ttaacagctt tcagccaggg acgtgttgta 180
tcctaggcag tggcccgccc ataggtcaca atgtcgaaga tcaacaaata cggtctcgaa 240
ctacactggg ctccagaatt tccatggatg tttgaggacg cagaggagaa gttggataac 300
cctagtagtt cagaggtgga tatgatttgc tccaccactg cgcaaaagct ggaaacagac 360
ggaatttgtc ctgaaaatca tgtgatggtg gattgtcgcc gacttcttaa acaagagtgt 420
tgtgtgcagt ctagcctaat acgtgaaatt gttatgaatg caagtccata tgatttggag 480
gtgctacttc aagatgcttt gcagtcccgt gaagcagttt tggttacaac ccccttaggt 540
atgtctttag aggcatgcta tgtgagaggt tgtaatccta aaggatggac catgggtttg 600
tttcggcgta gaagtgtgtg taacactggt cgttgcactg ttaataagca tgtggcctat 660
cagttatata tgattgatcc tgcaggtgtc tgtcttggtg caggtcaatt cgtgggttgg 720
gtcataccct tagcctttat gcctgtgcaa tcccggaaat ttattgttcc atgggttatg 780
tacttgcgta agcgtggcga aaagggtgct tacaataaag atcatggacg tggcggtttt 840
ggacatgttt atgattttaa agttgaagat gcttatgacc aggtgcatga tgagcctaag 900
ggtaagtttt ctaagaaggc ttatgcttta attagagggt atcgtggtgt taaaccactt 960
ctctatgtag accagtatgg ttgtgattat actggtagtc ttgcagatgg cttagaggct 1020
tatgctgata agacattgca agaaatgaag gcattatttc ctacttggag tcaggaactc 1080
ctttttgatg taattgtggc atggcatgtt gtgcgtgatc cacgttatgt tatgagattg 1140
cagagtgctg ctactatacg tagtgttgca tatgttgcta atcctactga agacttgtgt 1200
gatggttctg ttgttataaa agaacctgtg catgtttatg cagatgactc tattatttta 1260
cgtcaatata atttagttga cattatgagt catttttata tggaggcaga tacagttgta 1320
aatgcttttt atggtgttgc tttgaaagat tgcggttttg ttatgcagtt tggttacatt 1380
gattgcgaac aagactcgtg tgattttaaa ggttggattc ctggtaacat gatagatggt 1440
tttgcttgca ccacttgtgg tcatgtttat gaagtaggtg atttgatagc acaatcttca 1500
ggtgttttgc ctgttaaccc tgtattgcat actaagagtg cagcaggcta tggtggtttt 1560
ggttgtaaag attcttttac tctgtatggc caaactgtag tttattttgg aggttgtgtg 1620
tattggagtc cagcacgtaa tatatggatt cctatattaa aatcctctgt taagtcatat 1680
gacagtttgg tttatactgg agttttaggt tgcaaggcta ttgtaaagga aacaaatctc 1740
atttgcaaag ctttgtacct tgattatgtt caacacaagt gtggcaattt acaccaacgg 1800
gagttgctag gtgtttcaga tgtgtggcat aaacaattgc tattaaatag aggtgtttat 1860
aaacctctgt tagagaatat tgattatttt aatatgcggc gcgctaaatt tagtttagaa 1920
acttttactg tttgtgcaga tggctttatg ccttttcttt tagatgattt agttccacgc 1980
gcatattatt tggcagtaag tggtcaagca ttttgtgatt atgcagataa actttgccat 2040
gccgttgtgt ctaagagtaa agagttactt gatgtgtctc tggattcttt aggtgcagct 2100
atacattatt tgaattctaa gattgttgat ttggctcaac attttagtga ttttggaaca 2160
agtttcgttt ctaaaattgt tcatttcttt aagactttta ctactagcac tgctcttgca 2220
tttgcatggg ttttatttca tgttttgcat ggtgcttata tagtagtgga gagtgatata 2280
tattttgtta aaaacattcc tcgttatgct agtgctgttg cacaagcatt tcagagtgtt 2340
gctaaagttg tactggactc tttaagagtt acttttattg atggcctttc ttgttttaag 2400
attggacgta gaagaatttg tctttcaggc agaaaaattt atgaagttga gcgtggcttg 2460
ttacattcat cccaattgcc attagatgtt tatgatttaa ccatgcctag tcaagttcag 2520
aaagccaagc aaaaacctat ttatttaaaa ggttctggtt ctgatttttc attagcggat 2580
agtgtagttg aagttgttac aacttcactt acaccatgtg gttattctga accacctaaa 2640
gttgcagata aaatttgcat tgtggataat gtttatatgg ccaaggctgg tgacaaatat 2700
taccctgttg tggttgatga tcatgttgga ctcttggatc aagcatggag agttccttgt 2760
gctggaaggc gtgttacatt taaggaacag cctacagtaa aggagattat aagcatgcct 2820
aagattatta aggtttttta tgagcttgac aacgatttta atactatttt aaatactgcg 2880
tgtggagtgt ttgaagtgga tgatactgtt gatatggagg aattttatgc tgtggtgatt 2940
gatgccatag aagagaaact ttctccatgt aaggagcttg aaggtgtagg tgctaaagtt 3000
agtgcctttt tacagaaatt agaggataat cccctatttt tatttgatga ggctggcgag 3060
gaagttcttg ctcctaaatt gtattgtgcc tttacagctc ctgaagatga tgactttctt 3120
gaggaaagtg atgttgaaga agatgatgta gaaggtgagg aaactgattt aactgtcaca 3180
agtgctggac agccttgtgt tgctagtgaa caggaggagt cttctgaagt cttagaggac 3240
actttggatg atggtccaag tgtggagaca tctgattcac aagttgaaga agatgtagaa 3300
atgtcggatt ttgttgatct tgaatctgtg attcaggatt atgaaaatgt ttgttttgag 3360
ttttatacta cagagccaga atttgttaaa gttttgggtc tgtatgtgcc taaagcaact 3420
cgcaacaatt gctggttgcg atcagttttg gcagtgatgc agaaattgcc ctgtcaattt 3480
aaagataaaa atttgcagga tctttgggtg ttatacaagc aacagtatag tcagttgttt 3540
gttgatacct tggttaataa gatacctgct aatattgtac ttccacaagg tggttatgtt 3600
gctgattttg catattggtt tttaacctta tgtgattggc agtgtgttgc atactggaaa 3660
tgcattaaat gtgatttagc tcttaagctt aaaggcttgg atgctatgtt cttttatggt 3720
gatgttgttt cacatatatg caagtgtggt gagtctatgg tacttattga tgttgatgtg 3780
ccatttacag cccactttgc tcttaaagat aagttgtttt gtgcatttat tactaagcgt 3840
attgtgtata aagcagcttg tgttgtggat gttaatgata gtcattctat ggctgttgtt 3900
gatggtaaac aaattgatga tcatcgtatc actagtatta ctagtgataa gtttgatttt 3960
attattgggc atggtatgtc attttcaatg actacttttg aaattgccca attgtatggt 4020
tcttgtataa cacctaatgt gtgttttgtt aaaggtgata taattaaagt atctaagctt 4080
gttaaagcag aagttgttgt aaaccctgct aatggccata tggcacatgg tggtggtgtt 4140
gcaaaagcta ttgcagtagc agctggacag cagtttgtta aagagactac cgatatggtt 4200
aagtctaaag gagtttgtgc tactggagat tgttatgtct ctacaggggg caaattatgt 4260
aaaactgtgc ttaatgttgt tggacctgat gcgagaacac agggtaaaca aagttatgta 4320
ttgttagagc gtgtttataa acatcttaac aactatgact gtgttgttac aactttgatc 4380
tcagctggta tatttagtgt gccttctgat gtgtctttaa catatctact tggtactgct 4440
aagaaacaag ttgttcttgt tagcaataat caagaggatt ttgatcttat ttctaagtgt 4500
cagataactg ctgttgaggg cactaagaaa ttggcagcgc gtctttcttt taatgttgga 4560
cgttccattg tttacgaaac agatgctaat aagttgattt taatcaatga cgttgcattt 4620
gtttcgacat ttaatgtttt acaggatgtt ttatccttaa gacatgatat agcacttgat 4680
gatgatgcac gaaccttcgt tcagagcaat gttgatgttg tacctgaggg ttggcgtgtt 4740
gtcaataagt tttatcaaat taatggtgtt agaaccgtta agtattttga gtgtactgga 4800
ggcatagata tatgcagcca ggataaagtt tttggttatg tacagcaggg tatttttaat 4860
aaggctactg ttgctcaaat taaagccttg tttttggata aagtggacat cttgctaact 4920
gttgatggtg ttaatttcac taataggttt gtgcctgttg gtgaaagttt tggtaagagt 4980
ctaggaaatg tgttttgtga tggagttaat gtcacgaagc ataagtgtga tataaattat 5040
aaaggtaaag tctttttcca gtttgataat ctttctagtg aagatttaaa ggctgtaaga 5100
agttccttta attttgatca gaaggaattg cttgcctatt acaacatgct tgttaattgt 5160
tttaagtggc aggttgttgt taatggtaag tatttcactt ttaagcaagc taataacaat 5220
tgttttgtta atgtttcttg cttaatgctc cagagtttgc atctgacatt taaaattgtt 5280
caatggcaag aggcatggct tgaatttcgt tctggccgcc ctgctagatt tgtagctttg 5340
gttttggcca aaggtgggtt taaatttgga gatcctgctg attctagaga tttcttgcgt 5400
gttgtgttta gtcaagttga tttgactggg gcaatatgtg attttgaaat tgcatgtaaa 5460
tgtggtgtaa agcaggaaca gcgtactggt ctggacgctg ttatgcattt tggtacattg 5520
agtcgtgaag atcttgagat tggttatacc gtggactgtt cttgcggtaa aaagctaatt 5580
cattgtgtac gatttgatgt accattttta atttgcagta atacacctgc tagtgtaaaa 5640
ttacctaagg gtgtaggaag tgcaaatatt tttataggtg ataaggttgg tcattatgtt 5700
catgttaagt gtgaacaatc ttatcagctt tatgatgctt ctaatgttaa gaaggttaca 5760
gatgttactg gcaagttgtc agattgtctg tatcttaaaa atttgaaaca aacttttaaa 5820
tcggtgttaa ccacctatta tttggatgat gttaagaaaa ttgagtataa acctgacttg 5880
tcacaatatt attgtgacgg aggtaagtat tatactcagc gtattattaa agcccaattt 5940
aaaacattcg agaaagtaga tggtgtgtat actaatttta aattgatagg acacaccgtc 6000
tgtgacagtc ttaatgctaa gttgggtttt gatagctcta aagagtttgt tgaatataag 6060
attactgagt ggccaacagc tacaggtgat gtggtgttgg ctactgatga tttgtatgtt 6120
aagagatatg agaggggttg tattactttt ggtaaacctg ttatatggtt aagccatgag 6180
aaagcttccc tcaattcttt aacatatttt aatagacctt cattggttga tgataataaa 6240
tttgatgttt taaaagtgga tgatgttgac gatggtggtg acagctcaga gagtggtgcc 6300
aaagaaacca aagaaatcaa cattattaag ttaagtggtg ttaaaaaacc atttaaggtt 6360
gaagatagtg tcattgttaa tgatgatact agtgaaacca aatatgttaa gagtttgtct 6420
attgttgatg tgtatgatat gtggcttaca ggttgtaagt atgttgttag aactgctaat 6480
gctttgagca gagcagttaa cgtacctaca atacgtaagt ttataaaatt tggtatgact 6540
cttgttagta taccaattga tttgttaaat ttaagagaga ttaagcctgc tgttaatgtg 6600
gttaaagctg tgcgaaataa aatttctgta tgctttaatt ttattaaatg gctttttgtc 6660
ttattatttg gctggattaa aatatccgct gataataaag taatctacac cacagaaatt 6720
gcatcaaagc ttacgtgtaa gcttgtagct ttagctttta aaaatgcatt tttgacattt 6780
aagtggagta tggttgctag aggtgcttgc attatagcga ctatatttct attgtggttt 6840
aattttatat atgccaatgt aatttttagt gatttttatt tgcctaaaat cggtttcttg 6900
ccgacttttg ttggtaagat tgcacagtgg attaagaaca cttttagtct tgtaactatt 6960
tgtgatctat attccatgca ggatgtgggt tttaagaatc agtattgtaa tggaagtatt 7020
gcatgtcagt tctgcttggc aggatttgat atgttagata attataaagc cattgatgta 7080
gtacagtatg aagctgatag gagagcattt gttgattata caggtgtgtt aaagattgtc 7140
attgaattga tagttagtta cgccctgtat acggcatggt tttatccatt gtttgccctt 7200
atcagtattc agatcttgac cacttggctg cctgagcttt ttatgcttag tacattacat 7260
tggagtttta ggttgctggt ggctttagct aatatgttac cagcacatgt gtttatgagg 7320
ttttatatta ttattgcctc ttttattaag ctctttagct tgtttaggca tgttgcctat 7380
ggttgtagta aatctggttg tttgttttgt tacaagagga atcgtagtct acgtgttaaa 7440
tgtagtacta tcgttggtgg catgatacgc tattacgatg ttatggctaa tggtggcact 7500
ggcttttgtt caaaacatca atggaattgc attgattgtg attcttataa accaggtaat 7560
acttttatta ctgttgaggc cgctcttgat ctatctaagg aattgaaacg gcccattcag 7620
cctacagatg ttgcttatca tacggttact gatgttaagc aagttggttg ttctatgcgc 7680
ttgttctatg atcgtgatgg acagcgcaca tatgatgatg ttaatgctag tttgtttgtg 7740
gattatagta atttgctaca ttctaaggtt aagagtgtgc ctaatatgca tgttgtggta 7800
gtggaaaatg atgctgataa agccaatttt ctgaatgctg ctgtatttta tgcacagtct 7860
ttgtttagac ctattttaat ggttgataaa aatctgataa ctactgctaa cactggtacg 7920
tctgttacag aaactatgtt tgatgtttat gtggatacat ttttgtctat gtttgatgtg 7980
gataaaaaga gtcttaatgc tttaatagca actgcgcatt cttctataaa acagggtacg 8040
cagatttata aagttttgga taccttttta agctgtgctc gtaaaagttg ttctattgat 8100
tcagatgttg atactaagtg tttagctgat tctgtcatgt ctgctgtatc ggcaggtctt 8160
gaattgacgg atgaaagttg taataacttg gtgccaacat atttgaagag tgacaacatt 8220
gtggcagctg atttaggtgt tctgattcaa aattctgcaa agcatgtgca gggtaatgtt 8280
gctaaaatag ctggtgtttc ctgtatatgg tctgtggatg cttttaatca gtttagttct 8340
gatttccagc ataaattgaa gaaagcatgt tgtaaaactg gtttgaaact gaagcttact 8400
tataataagc agatggctaa tgtctctgtt ttaactacac cctttagtct taaagggggt 8460
gcagttttta gttattttgt ttatgtgtgt tttgtgttga gtttggtctg ttttattgga 8520
ctgtggtgct taatgcccac ttacacagta cacaaatcag attttcagct tcccgtttat 8580
gccagttata aagttttaga taatggtgtt attagagatg ttagcgttga agatgtttgt 8640
ttcgctaaca aatttgaaca atttgatcaa tggtatgagt ctacatttgg tctaagttat 8700
tatagtaaca gtatggcttg tcccattgtt gttgctgtaa tagatcagga ttttggctct 8760
acagtgttta atgtccctac caaagtgtta cgatatggtt atcatgtgtt gcactttatt 8820
acacatgcac tttctgctga tggagtgcag tgttatacgc cacatagtca aatatcgtat 8880
tctaattttt atgctagtgg ctgtgtgctt tcctctgctt gcactatgtt tacaatggcc 8940
gatggtagtc cacaacctta ttgttataca gaggggctta tgcaaaatgc ttctctgtat 9000
agttcattgg tacctcacgt gcggtataat cttgctaatg ctaaaggttt tatccgtttt 9060
ccagaagtgt tgcgagaagg gcttgtacgt atcgtgcgta ctcgttctat gtcgtattgc 9120
agagttggat tatgtgagga agctgatgag ggtatatgct ttaattttaa tggttcttgg 9180
gtgcttaata atgattatta tagatcattg cctgggacct tttgtggtag agatgttttt 9240
gatttaattt atcagctatt taaaggttta gcacagcctg tggatttttt ggcattgact 9300
gctagttcca ttgctggtgc tatactcgct gtaattgttg ttttggtgtt ttattaccta 9360
ataaagctta aacgtgcttt tggtgattac accagtgttg tttttgttaa cgtgattgtg 9420
tggtgtgtaa attttatgat gctttttgtg tttcaagttt accccatact ttcttgtgta 9480
tatgctattt gttattttta tgccacgctt tatttccctt cggagataag tgtgataatg 9540
cacttacaat ggctagttat gtatggcact attatgcctt tatggttttg tttgctatat 9600
atagctgttg ttgtttcaaa tcatgctttt tgggtatttt cttactgcag aaagcttggt 9660
acttctgttc gtagtgatgg tacatttgaa gaaatggctc tcactacttt tatgattaca 9720
aaagattctt attgtaagct taagaattct ttgtctgatg ttgcttttaa tagatatttg 9780
agtttgtata ataaatatag gtattacagc ggtaaaatgg atactgctgc atatagggag 9840
gctgcttgct ctcagttggc taaagcaatg gacacattta ccaataataa tggtagtgat 9900
gtgctttacc aaccgcctac tgcttccgtc tcaacttcat tcttgcaatc tggtattgtg 9960
aaaatggtaa atcctacttc taaggtagaa ccatgtgttg tcagtgttac ctatggtaat 10020
atgacattga atggtttatg gttggatgac aaggtctact gtcccagaca tgtaatatgt 10080
tctgcttcag atatgactaa tccagattat acaaatttgt tgtgtagagt aacatcaagt 10140
gattttactg tattgtttga tcgtctaagc cttacagtga tgtcttatca aatgcggggt 10200
tgtatgcttg ttcttacagt gaccctgcaa aattctcgta cgccaaaata tacatttggt 10260
gtggttaaac ctggtgagac ttttactgtt ttagctgctt ataacggcaa accacaagga 10320
gcctttcatg taactatgcg tagtagttat accattaagg gttccttttt atgcggatct 10380
tgtggatctg ttggttatgt aataatgggt gattgtgtta aatttgttta tatgcatcaa 10440
ttggagctta gtactggttg tcatactggt actgacttca atggggattt ttatggtcct 10500
tataaggatg ctcaggttgt tcagttgctc attcaggatt atatacaatc tgttaatttt 10560
gtagcatggc tttatgctgc tatacttaac aattgtaatt ggtttgtaca aagtgataag 10620
tgttctgtag aagattttaa tgtgtgggct ctgtccaatg gatttagcca agttaaatct 10680
gaccttgtta tagatgcttt agcttctatg actggtgtgt ctttggaaac actgttggct 10740
gctattaagc gtcttaagaa tggtttccaa ggacgtcaga ttatgggtag ttgctctttt 10800
gaggatgaat tgacacctag cgatgtttat caacaactcg ctggtatcaa gttacaatca 10860
aaacgcacta gattgtttaa aggcactgtt tgttggatta tggcttctac atttttgttt 10920
agttgcataa ttacagcatt tgtgaaatgg actatgttta tgtatgtaac tactaatatg 10980
tttagtatta cgttttgtgc actttgtgtt ataagtttgg ccatgttgtt ggttaagcat 11040
aagcatcttt atttgactat gtatataact cctgtgcttt ttacactgtt gtataacaac 11100
tatttggttg tgtacaagca tacatttaga ggctatgtct atgcatggct atcatattat 11160
gttccatcag ttgagtacac ttatactgat gaagttattt atggcatgtt attgcttgta 11220
ggaatggtct ttgttacatt acgtagcatt aaccatgatt tgttttcttt tataatgttt 11280
gttggtcgtt tgatttctgt tttctctttg tggtacaagg gttctaactt agaggaagaa 11340
attcttctta tgttggcttc cctttttggt acttacacat ggacaacagt tttatctatg 11400
gctgtagcaa aggttattgc taagtgggtt gctgtgaatg tcttgtattt cacagatata 11460
cctcaaatta agatagtgct tttgtgctat ttgtttattg gttatattat tagctgttat 11520
tggggcttgt tttccttgat gaacagtttg tttagaatgc ctttgggtgt ttataattat 11580
aaaatttcag tacaggaatt aagatatatg aatgctaatg gattgcgccc tcctaagaat 11640
agttttgaag cccttatgct taattttaag ctgttgggta ttggaggtgt tccaatcatt 11700
gaagtatctc aatttcaatc aaaattgact gatgtcaaat gtgctaatgt cgtcttgctt 11760
aattgcttgc aacatttgca tgttgcttct aattctaagt tgtggcatta ttgtagcact 11820
ttgcacaatg aaatacttgc cacttcggat ctgagtgttg cttttgaaaa gcttgctcag 11880
ttattaattg ttttgtttgc taatccagct gctgtggata gcaagtgcct gactagtatt 11940
gaagaagttt gcgatgatta cgcaaaggac aatactgttt tgcaggcttt acagagtgaa 12000
tttgttaata tggctagctt cgttgaatat gaagttgcta agaaaaatct tgatgaggcg 12060
cgttttagtg gttctgctaa tcaacagcag ttaaaacagc tagagaaagc ctgtaatatt 12120
gctaaatctg cttatgaacg cgaccgtgct gtagcaaaaa agttggagcg tatggctgat 12180
ttggctctca ctaatatgta taaagaagct agaattaatg ataagaagag taaggttgtt 12240
tctgccttgc aaactatgct ttttagtatg gtgcgtaagt tagataatca agctctgaat 12300
tcaatattag ataacgctgt gaagggttgt gtaccattga atgcaatacc ttcattggca 12360
gcaaatactc tgaatataat tgtaccagat aaaagtgttt atgaccaggt agttgataat 12420
gtctatgtta cctatgcggg taatgtatgg cagattcaaa ctatccagga ttcagatggt 12480
acaaataagc agttgaatga gatatctgat gattgtaact ggccactagt tattattgca 12540
aatcggtata atgaggtatc tgctactgtt ttgcaaaata atgaattaat gcctgctaag 12600
ttgaaaattc aggttgttaa tagtggtcca gatcagactt gtaatacacc tactcaatgt 12660
tactataata atagtaacaa tgggaagatt gtttatgcta tacttagtga tgttgatggt 12720
cttaagtata caaaaattct taaagatgat ggcaattttg ttgttttgga gttagatcct 12780
ccttgtaaat ttactgttca agatgctaaa ggtcttaaaa ttaagtacct ttattttgta 12840
aaaggttgta acacactagc aagaggctgg gttgttggta caatttcttc tacagttaga 12900
ttgcaagctg gaactgctac tgaatatgct tccaactcat ctatattgtc tttatgtgcg 12960
ttttctgtag atcctaagaa aacgtattta gattttatac aacaaggagg aacacctatt 13020
gccaattgtg ttaaaatgtt gtgtgaccat gctggtaccg gtatggccat tactgttaaa 13080
cccgatgcta ccactagtca ggattcatat ggtggtgcgt ctgtttgtat atattgccgc 13140
gcacgagttg aacacccaga tgttgatggg ttgtgcaaat tacgcggcaa gtttgtacaa 13200
gtgcctgtag gtataaaaga tcctgtgtct tatgttttga cacatgatgt ttgtcgagtt 13260
tgtggatttt ggcgggatgg aagttgttca tgtgttagca ctgacactac tgttcaatca 13320
aaagatacta attttttaaa cgggttcggg gtacgagtgt agatgcccgt ctcgtaccct 13380
gcgccagtgg tttatctact gatgtacaat taagggcatt tgatatttac aatgctagtg 13440
ttgctggcat tggtttacat ttaaaagtta attgttgccg ttttcagcgt gttgatgaga 13500
acggtgataa attagatcag ttctttgttg ttaagaggac agatctgact atatataata 13560
gagagatgaa atgctatgag cgtgtaaaag attgtaagtt tgtggctgaa cacgatttct 13620
ttacatttga tgtagaaggt agtcgtgtgc cacacattgt acgcaaggat ttaacaaagt 13680
atactatgtt ggatctttgc tatgcattgc gacattttga tcgcaatgat tgcatgctgc 13740
tttgtgacat tctctctata tatgctggtt gtgaacaatc ctactttact aagaaggatt 13800
ggtatgattt tgttgaaaat cctgatatta ttaatgtgta taaaaagcta ggacctattt 13860
ttaatagagc cctagttagc gctactgagt ttgcggacaa attggtggag gtaggcttag 13920
taggcgtttt aacacttgat aatcaagatt taaatggtaa atggtatgat tttggtgact 13980
atgttattgc agccccagga tgtggtgttg ctatagcaga ttcttattat tcttatatca 14040
tgcctatgct gaccatgtgt catgcattgg attgcgaatt gtatgtgaat aatgcttata 14100
gactatttga tcttgtacag tatgatttta ctgattacaa gcttgaattg tttaataagt 14160
attttaagca ctggagtatg ccatatcatc ctaacactgt tgattgtcag gatgatcggt 14220
gtattataca ttgtgctaat tttaacatac tttttagtat ggttttacct aatacatgtt 14280
ttgggcctct tgttaggcaa atttttgtgg atggtgtgcc ttttgttgtt tcaattggct 14340
accattataa agaacttggt attgtgatga atatggatgt ggatacacat cgttatcgct 14400
tgtctttaaa agacttgctt ttatatgctg ctgatccagc tttgcatgta gcttctgcta 14460
gtgcattgta tgatttacgc acttgctgtt ttagtgttgc cgctataaca agcggtgtaa 14520
aatttcaaac agttaaacct ggtaatttta atcaggattt ttatgatttt gttttaagta 14580
aaggcctgct taaagagggt agctcagttg atctgaagca ctttttcttt acacaggatg 14640
gtaatgctgc tattactgat tataattatt ataagtataa tttgcccacc atggtggaca 14700
ttaagcagtt gttgtttgtt ttggaagttg tttataagta ttttgagatt tatgatggtg 14760
ggtgtatacc ggcatcacaa gtcattgtta ataattatga taagagtgct ggctatccat 14820
ttaacaaatt tggaaaagcc aggctctatt atgaagcatt atcatttgag gaacaggatg 14880
aaatttacgc ttatactaag cgtaatgtcc tgccaacact tactcaaatg aatttgaaat 14940
atgctattag tgctaagaat agagcccgca ctgttgctgg tgtttccata cttagtacta 15000
tgactggcag aatgtttcat caaaaatgtt tgaaaagtat agcagctaca cgtggtgttc 15060
ctgtagttat aggcaccact aaattttatg gtggctggga tgatatgtta cgccgcctta 15120
ttaaagatgt tgacaatcct gtacttatgg gttgggatta tcctaagtgt gatcgtgcta 15180
tgccaaacct actacgtatt gttagtagtt tggtattagc ccgaaaacat gagacatgtt 15240
gttcgcaaag cgataggttt tatcgacttg cgaatgaatg cgcacaagtt ttgagtgaaa 15300
ttgttatgtg tggtggctgt tattatgtta agcctggtgg cactagtagt ggtgatgcaa 15360
ctactgcttt tgctaattca gtctttaaca tatgtcaagc tgtttcagcc aatgtatgtg 15420
ccttaatgtc atgcaatggc aataagattg aagatcttag tatacgtgct cttcagaagc 15480
gcttatactc acatgtgtat agaagtgata aggttgattc aacctttgtc acagaatatt 15540
atgaattttt aaataagcat tttagtatga tgattttgag tgatgatggg gttgtgtgtt 15600
ataattctga ttatgcgtcc aaagggtata ttgctaatat aagtgccttt caacaggtat 15660
tatattatca aaataacgtt tttatgtcag aatccaaatg ttgggttgaa catgacataa 15720
ataatggacc tcatgaattc tgttcacaac acacaatgct tgtaaagatg gatggtgacg 15780
atgtctacct tccatatcct aatcctagtc gtatattagg agctggatgt tttgtagatg 15840
atttgttaaa gactgatagt gttcttttaa tagaacgatt tgtaagtctt gcaatagatg 15900
cttatccact tgtgtatcat gaaaatgaag aataccaaaa ggtttttcgt gtttatttgg 15960
cgtatataaa gaagttgtac aatgacctgg gtaatcagat cttggatagc tacagtgtta 16020
ttttaagtac ttgtgatgga caaaagttca ctgatgagtc cttttacaag aacatgtatt 16080
taagaagtgc agttatgcag agtgttggag cttgcgtggt ctgctcttct caaacatcat 16140
tacgttgtgg cagttgcatc agaaagcctc ttctttgctg caagtgttgt tatgatcatg 16200
ttatggcgac tgatcataaa tatgtcttga gtgtttcacc atatgtgtgt aatgcaccag 16260
gatgtgatgt aaatgatgtt accaaattgt atctaggtgg tatgtcatat tattgtgaag 16320
accataagcc acaatattca ttcaagttgg taatgaatgg tctggttttt ggtctatata 16380
aacaatcttg tacaggatct ccgtacatag acgattttaa tcgtatagct agttgtaaat 16440
ggaccgatgt ggatgattac atactagcta atgaatgtac agagcgcttg aaattgtttg 16500
ctgcagaaac gcaaaaggca accgaggaag cctttaagca gagttatgca tcagcaacaa 16560
tacaagagat tgttagtgag cgcgaattga ttctctcttg ggagattgga aaagttaagc 16620
caccacttaa taaaaattat gtttttactg gctaccattt tactaaaaat ggtaagacag 16680
ttttaggtga gtatgttttt gataagagtg agttgactaa tggtgtgtat tatcgcgcca 16740
caaccactta taagctatct gtaggagatg tttttgtttt aacctctcat tcagtagcta 16800
atttaagtgc tcctacgctt gttccgcagg agaattatag tagtattaga tttgctagtg 16860
tttatagtgt gcttgagacg tttcagaaca atgttgttaa ttatcaacac attggtatga 16920
aacgttactg caccgtgcaa ggacctcctg gtacagggaa gtcacatctt gctattggtc 16980
ttgctgtatt ctattgtaca gcacgtgttg tatacacagc ggccagccat gcagctgttg 17040
acgcattgtg tgaaaaagca tataaatttt tgaatataaa tgattgcact cgtattgttc 17100
cggccaaggt cagggtggag tgctatgata agtttaaaat taatgacacc actcgtaagt 17160
atgtgtttac taccataaat gcattacctg agatggtgac tgatattgtt gttgtagatg 17220
aagttagtat gcttaccaat tatgagcttt ctgttattaa tgctcgtatt cgcgctaagc 17280
attatgttta tattggtgat cctgctcaat tgccagcacc acgtgtgtta ttgagcaagg 17340
gtacacttga acctaaatat tttaacactg ttactaagct catgtgttgc ttagggccag 17400
acatttttct tggtacatgt tatagatgtc ctaaggaaat cgttgataca gtgtccgcct 17460
tggtttatga aaataagctt aaggctaaga atgagagtag ttcattgtgt tttaaggtct 17520
attataaggg cgttacaaca catgaaagtt ctagtgctgt aaatatgcag cagatttatt 17580
tgattaataa gtttttgaag gctaaccctt tgtggcataa agctgttttt attagcccat 17640
ataatagtca gaactttgca gctaagcgtg ttttgggttt acaaacccaa accgtggatt 17700
ctgctcaagg ttctgaatat gattatgtta tatattcaca gactgcagaa acagcgcatt 17760
ctgtaaatgt taatcgcttc aatgttgcta ttactcgagc caagaaaggt attctttgtg 17820
ttatgagtaa tatgcagttg tttgaagcat tacagtttac tacattgacc ttagataaag 17880
tgccacaggc cgtcgaaact aaagttcaat gtagtactaa tttatttaaa gattgtagca 17940
agagttatag cggttatcac ccagctcatg ctccttcatt tttggcagta gatgacaaat 18000
ataaggcaac tggcgattta gccgtgtgtc ttggtattgg tgattctgct gttacatatt 18060
caagattaat atcactcatg ggttttaaat tggatgttac ccttgatggg tattgtaagc 18120
tttttataac taaagaagaa gctgttaaac gcgtgcgtgc ctgggttggc tttgatgctg 18180
aaggtgctca tgccacgcgt gatagcattg ggacaaattt cccacttcaa ttaggatttt 18240
ccacaggaat tgattttgtt gtggaagcca ctggtttgtt tgctgataga gatggttaca 18300
gctttaaaaa ggctgtggcg aaagctcctc ctggtgaaca atttaagcac ctcatccctt 18360
tgatgacgag aggtcatcgc tgggatgttg ttagacctag aatagtacaa atgtttgcag 18420
atcatttaat tgatctgtct gattgtgttg tgctagttac atgggcagcc aactttgagc 18480
tcacttgtct ccgctacttt gcaaaagtag ggcgtgagat ttcttgtaat gtatgcacta 18540
aacgtgccac agtttacaat tctagaactg gttactatgg ttgttggcgc catagtgtta 18600
catgtgatta cttgtataat ccacttattg ttgatattca acagtgggga tatattggtt 18660
ctttatcaag taatcatgat ttatattgta gtgtccataa aggagcacat gttgcttcct 18720
ctgatgctat aatgacacgg tgtttggccg tttatgattg cttttgcaat aatattaatt 18780
ggaatgtgga gtatcccatc atttcaaatg agttaagtat taatacctct tgtagggtct 18840
tgcagcgtgt gattcttaaa gctgccatgc tctgcaacag atatactttg tgttatgata 18900
ttggcaaccc aaaagcgatt gcctgtgtca aagattttga ttttaagttc tatgatgccc 18960
aaccaattgt taagtctgtt aagactcttt tgtattcttt tgaggcacat aaggactctt 19020
ttaaagacgg tttgtgtatg ttttggaact gtaatgtgga taagtatcca ccgaatgcag 19080
ttgtatgtag atttgacact agagtgttga ataatttaaa tcttcctggc tgtaatggag 19140
gtagtttgta tgttaataaa catgcattcc acactaaacc ctttgctagg gcagcctttg 19200
agcatttgaa gcctatgcca ttcttctatt attcagatac gccttgtgtg tatatggatg 19260
gcatggatgc taagcaggtt gattatgtac ctttgaaatc tgccacgtgc atcacaagat 19320
gcaatttagg tggtgcagtt tgtttaaaac atgctgaaga gtatcgtgag tacttagagt 19380
cttacaatac agctactaca gcaggtttta ctttttgggt ctataagaca tttgattttt 19440
ataatttgtg gaatacgttc accaagctac aaagcttgga gaatgttgta tataatttag 19500
tcaagactgg tcattataca ggacaggctg gtgaaatgcc ttgtgccatt ataaatgata 19560
aagttgtggc taagatcgat aaggaggatg ttgtcatttt tattaataat acaacatacc 19620
ctactaatgt ggccgttgaa ttatttgcca agcgcagtgt tcgacaccac ccagagctta 19680
agctctttag aaatttaaat atagacgtgt gttggaagca cgtcatttgg gattatgcta 19740
gagaaagtat attttgcagt aatacctatg gtgtctgcat gtatacagat ttaaagttca 19800
ttgataaatt gaatgtcctt tttgatggtc gtgataatgg tgctcttgaa gcttttaaac 19860
gttctaataa tggcgtttac atttccacga caaaagttaa gagtctttcg atgataagag 19920
gtccaccgcg tgctgaatta aatggcgtag tggtggacaa ggttggagac actgattgtg 19980
tgttttattt tgctgtgcgt aaagaaggtc aggatgtcat cttcagccaa ttcgacagcc 20040
tgggagtcag ctctaaccag agcccacaag gtaatctggg gagtaatggt aaacccggta 20100
atgtcggtgg taatgatgct ctgtcaatct ctactatctt tacacaaagc cgtgttatta 20160
gctcttttac atgtcgtact gatatggaaa aagattttat agctttagat caagatgtgt 20220
ttattcagaa gtatggtttg gaggactatg cctttgaaca cattgtttat ggtaacttca 20280
accagaagat tattggtggt ttgcatttgt taataggctt gtaccgaaga cagcaaactt 20340
ccaatctggt tgttcaggag tttgtttcat atgactccag catacactct tattttatca 20400
ctgacgagaa gagtggtggt agtaagagtg tttgcactgt tatagatatt ttgttggatg 20460
attttgtggc tcttgttaag tcacttaatc ttaattgtgt gagtaaggtt gttaatgtta 20520
atgttgattt taaagatttt cagtttatgc tttggtgtaa cgatgagaaa gttatgactt 20580
tctatcctcg tttgcaagct gcatctgact ggaagcctgg ttattctatg cctgtattat 20640
ataagtattt gaattctcca atggaaagag ttagtctctg gaattatggg aagccagtta 20700
ctttgcctac aggctgtatg atgaatgttg ctaagtatac tcagttatgt caatatctga 20760
atactacaac attagctgta cctgttaata tgcgagtttt gcatttaggt gcaggttcag 20820
aaaaaggagt agcaccgggt tctgcagttc ttaggcagtg gttgcctgct ggtactattc 20880
ttgtagataa cgatttatac ccatttgtta gtgacagtgt cgctacatat tttggggatt 20940
gtataacttt accctttgat tgtcaatggg atttgataat ttctgatatg tatgacccta 21000
ttactaagaa cataggggag tacaatgtga gtaaagatgg tttctttaca tacatttgtc 21060
atatgattcg agacaagtta gctctgggtg gcagtgttgc tataaaaata acagagtttt 21120
cttggaatgc agaattatat aagttaatgg ggtattttgc attttggact gtgttttgca 21180
caaatgcaaa tgcttcttct agtgaaggat ttttaattgg cataaattat ttgtgtaagc 21240
ccaaggttga gatagatgga aatgttatgc atgccaatta tttgttttgg agaaattcca 21300
cagtttggaa cgggggtgct tatagcctgt ttgatatggc taaattcccg cttaagttgg 21360
ctggtactgc cgtaataaat ttaagagcag accagattaa tgatatggtt tattcccttc 21420
ttgaaaaggg taaactactt attagagata caaataaaga agttttcgtt ggtgacagtt 21480
tggttaatgt aatctaaact ttaaaaatgg ctgtcgctta tgcagacaag cctaatcatt 21540
ttatcaattt tccacttacc cattttcagg gttttgtgtt aaattataaa ggtttacaat 21600
ttcaaattct cgatgaagga gtggattgta aaatacaaac agcgccacac attagtctta 21660
ctatgctgga catacagcct gaagactata aaagtgttga tgtcgctatt caagaagtta 21720
ttgatgatat gcattggggt gatggttttc agattaaatt tgagaatcct cacatcctag 21780
gaagatgcat agttttagat gttaaaggtg tagaagaatt gcatgacgat ttagttaatt 21840
acattcgtga taaaggttgt gttgctgacc aatccaggaa atggattggc cattgcacca 21900
tagctcaact cacggatgca gcactgtcca ttaaggaaaa tgttgatttt ataaacagca 21960
tgcaattcaa ttataaaatc accatcaacc cctcatcacc ggctagactt gaaatagtta 22020
agctcggtgc tgaaaagaaa gatggttttt atgaaaccat agttagtcac tggatgggaa 22080
ttcgttttga atacacatca cccactgata agctagctat gattatgggt tattgttgtt 22140
tagatgtggt acgtaaagag ctagaagaag gcgatcttcc cgagaatgat gatgatgctt 22200
ggtttaagct atcgtaccat tatgaaaaca attcttggtt cttccgacat gtctacagga 22260
aaagttttca tttccgtaag gcttgtcaaa atttagattg taattgtttg gggttttatg 22320
aatcttcagt tgaagaatat taaactcagt gaaaatgttt ttgcttccta gatttattct 22380
agttagctgc ataattggta gcttaggttt ttacaaccct cctaccaatg ttgtttcgca 22440
tgtaaatgga gattggtttt tatttggtga cagtcgttca gattgtaatc atattgttaa 22500
tatcaacccc cataattatt cttatatgga ccttaatcct gttctgtgtg attctggtaa 22560
aatatcatct aaagctggca actccatttt taggagtttt cactttaccg atttttataa 22620
ttacacaggc gaaggtcaac aaattatttt ttatgagggt gttaatttta cgccttatca 22680
tgcctttaaa tgcaaccgtt ctggtagtaa tgatatttgg atgcagaata aaggcttgtt 22740
ttatactcag gtttataaga atatggctgt gtatcgcagc cttacttttg ttaatgtacc 22800
atatgtttat aatggctccg cacaagctac agctctttgt aaatctggta gtttagtcct 22860
taataaccct gcatatatag ctcctcaagc taactctggg gattattatt ataaggttga 22920
agctgatttt tatttgtcag gttgtgacga gtatatcgta ccactttgta tttttaacgg 22980
caagtttttg tcgaatacaa agtattatga tgatagtcaa tattatttta ataaagacac 23040
tggtgttatt tatggtctca attctacaga aaccattacc actggttttg atcttaattg 23100
ttattattta gttttaccct ctggtaatta tttagccatt tcaaatgagc tattgttaac 23160
tgttcctacg aaagcaatct gtcttaataa gcgtaaggat tttacgcctg tacaggttgt 23220
tgattcgcgg tggaacaatg ccaggcagtc tgataacatg acggcggttg cttgtcaacc 23280
tccgtactgt tattttcgta attctactac caactatgtt ggtgtttatg atattaatca 23340
tggagatgct ggttttacta gcatacttag tggtttgtta tataattcac cttgtttttc 23400
gcagcaaggc gtttttaggt atgataatgt tagcagtgtc tggcctctct acccctatgg 23460
cagatgtccc actgctgctg atattaatat ccctgattta cccatttgtg tgtatgatcc 23520
gctaccagtt attttgcttg gcattctttt gggcgttgcg attgtaatta ttgtagtttt 23580
gttgttatat tttatggtgg ataatgttac taggctgcat gatgcttaga ccataatcta 23640
aacatgtttt tgatactttt aatttcctta ccaacggctt ttgctgttat aggagattta 23700
aagtgtactt cagatactag ttatattaat gataaagaca ccggtcctcc tcctataagt 23760
actgatactg ttgatgttac taatggtttg ggtacttatt atgttttaga tcgtgtgtat 23820
ttaaatacta cgttgtttct taatggttat taccctactt caggttccac atatcgtaat 23880
atggcactga agggaagtgt actattgagc agactatggt ttaaaccacc atttctttct 23940
gattttatta atggtatttt tgctaaggtc aaaaatacca aggttattaa agatcgtgta 24000
atgtatagtg agttccctgc tataactata ggtagtactt ttgtaaatac atcctatagt 24060
gtggtagtac aaccacgtac aatcaattca acacaggatg gttataataa attacaaggt 24120
cttttagagg tctctgtttg ccagtataat atgtgcgagt acccacaaac gatttgtcat 24180
cctaacctgg gtaatcatcg caaagaacta tggcatttgg atacaggtgt tgtttcctgt 24240
ttatataagc gtaatttcac atatgatgtg aatgctgatt atttgtattt tcatttttat 24300
caagaaggtg gtacttttta tgcatatttt acagacactg gtgttgttac taagtttttg 24360
tttaatgttt atttaggcat ggcgctttca cactattatg tcatgcctct gacttgtaat 24420
agtaaggtta agaatggttt tactttagaa tattgggtta cacctctcac ttctagacaa 24480
tatttactcg ctttcaatca agatggtatt atttttaatg ctgttgattg tatgagtgat 24540
tttatgagtg agattaagtg taaaacacaa tctatagcac cacctactgg tgtttatgaa 24600
ttaaacggtt acactgttca gccaatcgca gatgtttacc gacgtaaact taatcttccc 24660
aattgcaata tagaagcttg gcttaatgat aagtcggtgc cctctccatt aaattgggaa 24720
cgtaagacat tttcaaattg taattttaat atgagcagcc tgatgtcttt tattcaggca 24780
gactcattta cttgtaataa tattgatgct gctaagatat atggtatgtg tttttccagc 24840
ataactatag ataagtttgc tatacccaat ggcaggaagg ttgacctaca attgggtaat 24900
ttgggctatt tgcagtcatt taactataga attgatacta ctgcaacaag ttgtcagttg 24960
tattataatt tacctgctgc taatgtttct gttagcaggt ttaatccttc tacttggaat 25020
aagagatttg gttttataga agattctgtt tttaagcctc gacctgcagg tgttcttact 25080
aatcatgatg tagtttatgc acaacactgt ttcaaagctc ctaaaaattt ctgtccgtgt 25140
aaattgaatg gttcgtgtgt aggtagtggt cctggtaaaa ataatggtat aggcacttgt 25200
cctgcaggta ctaattattt aacttgtgat aatttgtgca ctcctgatcc tattacattt 25260
aaagctacag gtacttataa gtgcccccaa actaaatctt tagttggcat aggtgagcac 25320
tgttcgggtc ttgctgttaa aagtgattat tgtggaggca attcttgtac ttgccgacca 25380
caagcatttt tgggttggtc tgcagactct tgtttacaag gagacaagtg taatattttt 25440
gctaatttta ttttgcatga tgttaatagt ggtcttactt gttctactga tttacaaaaa 25500
gctaacacag acataattct tggtgtttgt gttaattatg acctctatgg tattttaggc 25560
caaggcattt ttgttgaggt taatgcgact tattataata gttggcagaa ccttttatat 25620
gattctaatg gtaatctcta cggttttaga gactacataa caaacagaac ttttatgatt 25680
cgtagttgct atagcggtcg tgtttctgcg gcctttcacg ctaactcttc cgaaccagca 25740
ttgctatttc ggaatattaa atgcaactac gtttttaata atagtcttac acgacagctg 25800
caacccatta actattttga tagttatctt ggttgtgttg tcaatgctta taatagtact 25860
gctatttctg ttcaaacatg tgatctcaca gtaggtagtg gttactgtgt ggattactct 25920
aaaaacagac gaagtcgtgg agcgattacc actggttatc ggtttactaa ttttgagcca 25980
tttactgtta attcagtaaa cgatagttta gaacctgtag gtggtttgta tgaaattcaa 26040
ataccttcag agtttactat aggtaatatg gaggagttta ttcaaacaag ctctcctaaa 26100
gttactattg attgtgctgc atttgtctgt ggtgattatg cagcatgtaa atcacagttg 26160
gttgaatatg gtagtttctg tgataacatt aatgccatac tcacagaagt aaatgaacta 26220
cttgacacta cacagttgca agtagctaat agtttaatga atggtgttac tcttagcact 26280
aagcttaaag atggcgttaa tttcaatgta gacgacatca atttttcccc tgtattaggt 26340
tgtctaggca gcgaatgtag taaagcttcc agtagatctg ctatagagga tttacttttt 26400
gataaagtaa agttatctga tgtcggtttt gttgaggctt ataataattg tacaggaggt 26460
gccgaaatta gggacctcat ttgtgtgcaa agttataaag gcatcaaagt gttgcctcca 26520
ctgctctcag aaaatcagat cagtggatac actttggctg ccacctctgc tagtctattt 26580
cctctttgga cagcagcagc aggtgtacca ttttatttaa atgttcagta tcgcattaat 26640
gggcttggtg tcaccatgga tgtgctaagt caaaatcaaa agcttattgc taatgcattt 26700
aacaatgccc tttatgctat tcaggaaggg ttcgatgcaa ctaattctgc tttagttaaa 26760
attcaagctg ttgttaatgc aaatgctgaa gctcttaata acttattgca acaactctct 26820
aatagatttg gtgctataag tgcttcttta caagaaattc tatctagact tgatgctctt 26880
gaagcggaag ctcagataga tagacttatt aatggtcgtc ttaccgctct taatgcttat 26940
gtttctcaac agcttagtga ttctacactg gtaaaattta gtgcagcaca agctatggag 27000
aaggttaatg aatgtgtcaa aagccaatca tctaggataa atttctgtgg taatggtaat 27060
catattatat cattagtgca gaatgctcca tatggtttgt attttatcca ctttagttat 27120
gtccctacta agtatgtcac agcgagggtt agtcctggtc tgtgcattgc tggtgataga 27180
ggtatagctc ctaagagtgg ttattttgtt aatgtaaata atacttggat gtacactggt 27240
agtggttact actaccctga acctataact gaaaataatg ttgttgttat gagtacctgc 27300
gctgttaatt atactaaagc gccgtatgta atgctgaaca cttcaatacc caaccttcct 27360
gattttaagg aagagttgga tcaatggttt aaaaatcaaa catcagtggc accagatttg 27420
tcacttgatt atataaatgt tacattcttg gacctacaag ttgaaatgaa taggttacag 27480
gaggcaataa aagtcttaaa tcagagctac atcaatctca aggacattgg tacatatgaa 27540
tattatgtaa aatggccttg gtatgtatgg cttttaatct gccttgctgg tgtagctatg 27600
cttgttttac tattcttcat atgctgttgt acaggatgtg ggactagttg ttttaagaaa 27660
tgtggtggtt gttgtgatga ttatactgga taccaggagt tagtaatcaa aacttcacat 27720
gacgactaag ttcgtctttg attcattgca ctgatctctt gttagatctt tttgcaatct 27780
agcatttgtt aaagttctta aggccacgcc ctattaatgg acatttggag acctgagaag 27840
aaatatctcc gttatattaa cggttttaat gtctcagaat tagaagatgc ttgttttaaa 27900
tttaactatc aatttcctaa agtaggatat tgtagagttc ctagtcatgc ttggtgccgt 27960
aatcaaggta gattttgtgc tacattcact ctttatggta aatccaaaca ttatgataaa 28020
tattttggag taataaatgg tttcacagca ttcgctaata ctgtagagga tgctgttaac 28080
aaactggttt tcttagctgt tgactttatt acctggcgca gacaggagtt aaatgtttat 28140
ggctgatgct tatcttgcag acactgtgtg gtatgtgggg caaataattt ttatagttgc 28200
catttgttta ttggttacaa tagttgtagt ggcatttttg gcaactttta aattgtgtat 28260
tcaactttgc ggtatgtgta ataccttagt actgtcccct tctatttatg tgtttaatag 28320
aggtaggcag ttttatgagt tttacaatga tgtaaaacca ccagtccttg atgtggatga 28380
cgtttaggta atccaaacat tatgagtagt aaaactacac cagcaccagt ttatatctgg 28440
actgctgatg aagctattaa attcctaaag gaatggaatt tttctttggg tattatacta 28500
ctttttatta caatcatatt gcaatttgga tatacaagtc gcagtatgtt tgtttatgtt 28560
attaagatga ttattttgtg gcttatgtgg ccccttacta taatcttaac tattttcaat 28620
tgcgtatacg cattgaataa tgtgtatctt ggcctttcta tagtttttac catagtggcc 28680
attattatgt ggattgtgta ttttgtgaat agtatcaggt tgtttattag aactggaagt 28740
ttttggagtt tcaacccaga aacaaacaac ttgatgtgta tagatatgaa aggaacaatg 28800
tatgttaggc cgataattga ggactatcat actctgacgg tcacaataat acgcggccat 28860
ctttacattc aaggtataaa actaggtact ggctattctt tggcagattt gccagcttat 28920
atgactgttg ctaaggttac acacctgtgc acatataagc gtggttttct tgacaggata 28980
agcgatacta gtggttttgc tgtttatgtt aagtccaaag tcggtaatta ccgactgcca 29040
tcaacccaaa agggttctgg catggacacc gcattgttga gaaataatat ctaaatttta 29100
aggatgtctt ttactcctgg taagcaatcc agtagtagag cgtcctctgg aaatcgttct 29160
ggtaatggca tcctcaagtg ggccgatcag tccgaccagt ttagaaatgt tcaaaccagg 29220
ggtagaagag ctcaacccaa gcaaactgct acctctcagc aaccatcagg agggaatgtt 29280
gtaccctact attcttggtt ctctggaatt actcagtttc aaaagggaaa ggagtttgag 29340
tttgtagaag gacaaggtgt gcctattgca ccaggagtcc cagctactga agctaagggg 29400
tactggtaca gacacaacag acgttctttt aaaacagccg atggcaacca gcgtcaactg 29460
ctgccacgat ggtattttta ctatctggga acaggaccgc atgctaaaga ccagtacggc 29520
accgatattg acggagtcta ctgggtcgct agcaaccagg ctgatgtcaa taccccggct 29580
gacattgtcg atcgggaccc aagtagcgat gaggctattc cgactaggtt tccgcctggc 29640
acggtactcc ctcagggtta ctatattgaa ggctcaggaa ggtctgctcc taattccaga 29700
tctacttcgc gcacatccag cagagcctct agtgcaggat cgcgtagtag agccaattct 29760
ggcaatagaa cccctacctc tggtgtaaca cctgacatgg ctgatcaaat tgctagtctt 29820
gttctggcaa aacttggcaa ggatgccact aaacctcagc aagtaactaa gcatactgcc 29880
aaagaagtca gacagaaaat tttgaataag ccccgccaga agaggagccc caataaacaa 29940
tgcactgttc agcagtgttt tggtaagaga ggccctaatc agaattttgg tggtggagaa 30000
atgttaaaac ttggaactag tgacccacag ttccccattc ttgcagaact cgcacccaca 30060
gctggtgcgt ttttctttgg atcaagatta gagttggcca aagtgcagaa tttatctggg 30120
aatcctgacg agccccagaa ggatgtttat gaattgcgct ataacggcgc aattaggttt 30180
gacagtacac tttcaggttt tgagaccata atgaaggtgc tgaatgagaa tttgaatgcc 30240
tatcaacaac aagatggtat gatgaatatg agtccaaaac cacagcgtca gcgtggtcat 30300
aagaatggac aaggagaaaa tgataatata agtgttgcag tgcccaaaag ccgcgtgcag 30360
caaaataaga gtagagagtt gactgcagag gacatcagcc ttcttaagaa gatggatgag 30420
ccctatactg aagacacctc agaaatataa gagaatgaac cttatgtcgg catctggtgg 30480
taacccctcg cagaaaagtc gagataaggc actctctatc agaatggatg tcttgctgct 30540
ataatagata gagaaggtta tagcagacta tagattaatt agttgaaagt tttgtgttgt 30600
aatgtatagt gttggagaaa gtgaaagact tgcggaagta attgccgaca agtgcccaag 30660
ggaagagcca gcatgttaag ttaccaccca gtaattagta aatgaatgaa gttaattatg 30720
gccaattgga agaatcac 30738
<210> 9
<211> 27317
<212> DNA
<213> Human coronavirus 229E
<400> 9
acttaagtac cttatctatc tacagataga aaagttgctt tttagacttt gtgtctactt 60
ttctcaacta aacgaaattt ttgctatggc cggcatcttt gatgctggag tcgtagtgta 120
attgaaattt catttgggtt gcaacagttt ggaagcaagt gctgtgtgtc ctagtctaag 180
ggtttcgtgt tccgtcacga gattccattc tacaaacgcc ttactcgagg ttccgtctcg 240
tgtttgtgtg gaagcaaagt tctgtctttg tggaaaccag taactgttcc taatggcctg 300
caaccgtgtg acacttgccg tagcaagtga ttctgaaatt tctgcaaatg gctgttctac 360
tattgcgcaa gccgtccgcc gttatagcga ggccgctagc aatggtttta gggcatgccg 420
atttgtttca ttagatttgc aggattgcat cgttggcatt gcagacgata catatgttat 480
gggtctgcat ggcaatcaga cgttgttttg caacataatg aaattttctg accgtccttt 540
tatgcttcat gggtggttgg ttttttccaa ttcaaattac cttttggagg aatttgatgt 600
tgtcttcggt aagagaggtg gtggtaatgt gacatacact gaccagtatc tctgtggcgc 660
cgatggcaaa cctgttatga gtgaagattt atggcagttt gttgaccatt tcggtgagaa 720
cgaagaaatt atcatcaatg gtcatactta cgtttgtgct tggcttacta agcgtaagcc 780
cttagattac aaacgtcaga acaaccttgc cattgaagag attgaatatg tgcatggtga 840
tgctttgcat acactacgca atggttctgt tcttgaaatg gctaaggaag tgaagacatc 900
tagtaaagtt gtgttaagcg atgctcttga caaactttac aaagtctttg gttctcctgt 960
tatgacaaat ggttccaaca tcctagaggc ctttactaaa cctgtgttta ttagtgcatt 1020
agttcaatgt acttgtggta ccaagtcttg gtctgttggt gattggaccg gttttaaatc 1080
ctcttgttgc aacgtgatca gtaataaact gtgtgttgtt cccggtaatg ttaaacctgg 1140
tgatgctgtg attaccactc agcaagctgg tgctggtatt aagtattttt gtggcatgac 1200
tcttaagttt gttgcaaata ttgaaggtgt ctctgtttgg agagtgattg ctcttcagag 1260
tgtggattgc tttgttgctt cttccacttt tgtagaagag gaacatgtta atagaatgga 1320
tacattctgc ttcaatgtac gcaatagtgt tactgatgag tgtcgtctgg ccatgttggg 1380
tgctgaaatg actagtaatg tcagaagaca agttgcttca ggtgtcatag acattagtac 1440
cggttggttt gatgtttatg atgacatctt tgctgaaagc aaaccatggt ttgttcgcaa 1500
ggctgaagac atttttggcc cttgttggtc cgctcttgct tctgcactta aacaacttaa 1560
agtcactaca ggtgaacttg tgagatttgt taagtctatt tgcaattcag ctgttgctgt 1620
cgtgggtggt actatacaaa ttctcgctag tgtgcctgag aagtttttga atgcgtttga 1680
cgtgtttgtc acagctattc aaactgtctt tgactgtgct gttgaaactt gtactattgc 1740
cggtaaagca tttgacaagg tttttgacta tgttttgctt gataatgcgc ttgtaaaact 1800
tgtcaccaca aagcttaagg gtgttcgtga acgtggcctt aataaagtta agtatgcaac 1860
agttgttgtt ggttccactg aagaagttaa atcttcacgt gttgaacgta gcactgctgt 1920
acttacaatc gccaacaatt attccaaact ttttgatgaa gggtatactg ttgtaattgg 1980
cgatgtggcg tactttgtta gtgacggcta cttccgtctt atggccagtc caaatagtgt 2040
gttgactact gcagtctata aaccattgtt tgcttttaat gtgaatgtta tgggtactag 2100
acctgaaaaa tttccaacca ctgtgacttg tgaaaattta gagtctgctg ttttgtttgt 2160
taatgacaaa attactgaat tccaattgga ttacagtatt gatgtcattg ataatgaaat 2220
aattgtcaaa cctaatatca gcctatgtgt tccactttat gtgagagact atgttgacaa 2280
atgggatgat ttttgcagac aatatagtaa cgagtcttgg tttgaggatg attacagggc 2340
ttttatcagt gttttggaca tcactgatgc tgctgtgaaa gctgcagagt ctaaagcttt 2400
cgttgatact attgttccac cttgcccatc tattttgaaa gttatagatg gaggcaaaat 2460
atggaatggt gttattaaaa atgttaactc tgttagagac tggcttaagt ctttgaagtt 2520
aaatctcaca caacagggtt tgcttggaac atgtgcaaag cgttttaaac gttggcttgg 2580
cattttgcta gaggcctata atgcgttttt agacactgtg gtttctactg ttaaaattgg 2640
tggcttgacc tttaaaacat atgcttttga taaaccttac attgtgatac gtgatatcgt 2700
gtgtaaggtt gaaaataaaa cagaagcaga atggattgag ctttttccac ataatgacag 2760
gattaagtct tttagtactt tcgagagtgc ttacatgcca attgcagacc ctacacattt 2820
tgacattgaa gaagttgaac ttttagatgc agagtttgta gaaccaggct gtggtggtat 2880
tttggcagta atagatgagc acgtctttta taagaaggat ggtgtttatt atccatcaaa 2940
tggtactaac attctacctg ttgcatttac aaaagccgct ggtggtaaag tttcattttc 3000
tgatgacgtt gaagtaaaag acattgaacc tgtttacaga gtcaagcttt gctttgagtt 3060
tgaagatgaa aaacttgtag atgtttgtga aaaggcaatt ggcaagaaaa ttaaacatga 3120
aggtgactgg gatagctttt gtaagactat tcaatcagca ctttctgttg tttcttgcta 3180
tgtaaatcta cctacttatt acatttatga tgaagaaggc ggtaatgact tgagtttgcc 3240
cgttatgatt tctgaatggc ctctttctgt tcaacaagct caacaagaag ctactttacc 3300
tgatattgct gaggatgttg ttgaccaagt tgaagaagtc aatagcattt ttgacattga 3360
gacagtggat gttaaacatg atgtgagtcc ttttgaaatg ccatttgaag agttaaatgg 3420
tttaaagata ctcaaacaat tggataacaa ctgctgggtt aactcagtta tgttacaaat 3480
acaattaact ggtatacttg atggtgacta tgctatgcag ttttttaaaa tgggccgagt 3540
tgccaagatg attgaacgct gctacactgc tgagcaatgt atacgtggtg ctatgggtga 3600
tgttggtttg tgtatgtata gactgcttaa agacttacac actggtttta tggttatgga 3660
ttataaatgt agttgtacca gtggtaggct tgaagaatcg ggagctgttt tgttttgtac 3720
gcccactaag aaggcgtttc cttatggtac ttgtctaaat tgtaacgcac ctcgcatgtg 3780
tacaattagg cagttacaag gtaccataat atttgtgcaa caaaaaccag aacctgttaa 3840
tcctgtttct tttgttgtta aaccagtctg ctcatcaatt tttcgtggtg ctgtgtcttg 3900
tggtcattac cagactaaca tctattcaca aaatttgtgt gtggatggtt ttggtgttaa 3960
caagattcag ccctggacaa atgatgcact taatactatt tgtattaagg atgcagatta 4020
taatgcaaaa gttgaaatat ctgttacacc aattaaaaat acagttgata caacacctaa 4080
ggaagaattt gttgttaaag agaagttgaa cgccttcctc gttcatgaca atgtagcttt 4140
ctaccaaggt gatgttgata ctgttgttaa tggtgttgac tttgacttta ttgtaaatgc 4200
tgctaatgag aaccttgctc atggtggagg acttgccaaa gctttagatg tgtacactaa 4260
aggtaaactt caacgtttat ctaaagaaca cattggatta gcgggtaaag taaaagttgg 4320
tacaggagtt atggttgagt gtgatagcct tagaattttt aatgttgttg gtccacgcaa 4380
gggtaaacat gaacgtgatt tactcataaa agcttacaac actattaata atgaacaagg 4440
cacaccttta acaccaattt tgagctgtgg tatttttggt atcaaactcg aaacttcatt 4500
agaagttttg cttgatgttt gtaatacaaa agaagttaaa gtttttgttt atacagacac 4560
agaggtttgt aaggttaagg attttgtgtc tggtttagtg aatgttcaaa aagttgagca 4620
acctaaaata gaaccaaaac cagtgtccgt aattaaagtt gcacccaagc cttacagggt 4680
agatggtaaa tttagttact ttacagaaga cttgttgtgt gtcgctgatg acaaacccat 4740
tgttttgttt actgactcta tgcttacttt ggatgaccgt ggtttagctc tagacaatgc 4800
acttagtggt gtgcttagtg ctgctattaa ggattgtgtt gacataaata aagctatacc 4860
ttctggtaat cttattaagt ttgatatagg ttctgttgtt gtctacatgt gtgttgtgcc 4920
atccgaaaag gacaaacatt tagataataa tgttcaacga tgcacacgta agttgaatag 4980
acttatgtgt gatatagttt gtactatacc agctgactac atcttgccat tggtgttgtc 5040
tagtttgact tgtaatgttt cttttgtagg tgaacttaaa gctgctgaag ctaaagttat 5100
aactataaag gtgacagagg atggtgttaa tgttcatgat gtgaccgtga caacagacaa 5160
gtcatttgaa caacaagttg gtgttattgc tgataaggac aaagatcttt ctggtgcagt 5220
accaagtgat cttaacacat ctgaattgct tactaaagca atagatgttg attgggtcga 5280
attttatggc tttaaagatg ctgttacttt tgcaacagtt gatcatagtg cttttgccta 5340
tgaaagtgct gttgttaatg gtattagagt gttaaaaact agtgataata attgttgggt 5400
gaatgctgtt tgtattgcac tacagtattc gaaaccccat tttatttcac aaggtcttga 5460
tgctgcgtgg aataaatttg ttttaggcga tgttgaaatt tttgttgcat ttgtttacta 5520
tgttgcaaga ctaatgaaag gtgacaaggg tgatgctgaa gacactttga ctaagttgtc 5580
taagtatctt gctaatgaag ctcaagttca attagaacat tatagttctt gtgttgaatg 5640
tgatgctaaa tttaaaaact ctgttgcatc tatcaattct gctatagttt gtgctagtgt 5700
caaacgtgat ggtgtgcaag ttggttattg tgtccatggt attaagtact attcacgtgt 5760
tagaagtgtt agaggtagag ctattatagt cagtgtcgaa cagcttgaac cgtgtgctca 5820
gtctagactt ttgagtggtg ttgcttatac tgctttttct ggacctgttg acaaaggtca 5880
ttatactgtt tatgatactg caaagaaatc aatgtatgat ggtgatcgtt ttgttaaaca 5940
tgatctttct ctgctgtctg tcacatcagt tgttatggtt ggtggttatg ttgcacctgt 6000
taatacagtg aaacctaaac cagtcattaa tcaacttgat gaaaaggcac agaagttctt 6060
tgattttggt gattttttga ttcataattt tgttattttt ttcacatggt tattgagtat 6120
gtttactttg tgtaaaactg cagtaactac aggtgatgtt aaaataatgg ccaaagcacc 6180
acaaaggacg ggtgttgttt taaaacgtag tcttaaatat aacttaaaag cgtcagcagc 6240
tgttcttaaa tctaagtggt ggctgcttgc taagtttacg aaactactgt tactcatata 6300
tacattgtac tcagtagttt tgctttgtgt acgttttgga ccgtttaatt tttgtagtga 6360
gactgttaat ggttatgcta agtcaaactt tgtcaaggat gattactgtg atggttcatt 6420
gggctgcaag atgtgtcttt ttggttacca agagttaagt caatttagcc atttggatgt 6480
tgtgtggaag catataacag accctttgtt tagtaatatg caacctttca ttgtcatggt 6540
tttgctgctt atatttggtg acaattattt gagatgcttc ttgctgtatt ttgttgctca 6600
gatgataagc acagttggtg tttttctagg ttacaaggaa acaaattggt tcttgcactt 6660
tattccattt gatgttattt gtgatgaact gcttgtcact gttattgtta ttaaggttat 6720
ttcttttgtc agacatgtgc tttttggttg tgaaaaccca gattgtattg cgtgttctaa 6780
gagtgctaga cttaagagat tccctgttaa cacaattgtc aatggtgtgc aacgttcatt 6840
ttatgttaat gcaaatggtg gtagtaagtt ttgtaagaaa catagatttt tctgtgttga 6900
ttgtgactct tatggttatg gcagcacgtt tataacaccc gaagtttcta gagaacttgg 6960
taacattacc aaaacaaatg tgcaaccaac agggccggcc tatgtcatga ttgacaaagt 7020
ggagtttgaa aatggttttt acagattgta ttcctgtgaa acattttggc gttacaactt 7080
tgatataact gaaagcaagt attcttgcaa agaggttttt aaaaattgta atgttttgga 7140
tgatttcatc gtgtttaaca ataatgggac caatgtaacg caggttaaaa atgctagtgt 7200
ttacttttca cagttgttgt gtaggcccat taaattagtt gacagtgaac ttttgtccac 7260
tttgtcagtt gattttaatg gtgtcttaca caaggcatac attgatgtac tacgtaatag 7320
ctttggtaaa gatcttaatg ctaatatgtc tttagccgag tgcaagagag ctttaggcct 7380
gtctattagt gatcatgaat ttactagtgc tatttctaat gcacatcgtt gtgacgtgtt 7440
gttatctgat ttgtcattta acaactttgt cagttcgtat gctaaacctg aggaaaaatt 7500
atcagcttat gacttggcgt gttgtatgcg tgcaggtgct aaggttgtta atgccaatgt 7560
tctgacaaag gaccaaactc ctattgtttg gcatgcaaag gattttaaca gtctttctgc 7620
tgaaggtcgc aagtatattg taaaaactag caaagctaag ggtttgactt tcttgttgac 7680
aattaatgaa aaccaagctg tcacgcaaat acctgcaact agcattgttg ctaagcaagg 7740
tgctggtgat gctggccatt cattaacatg gctgtggcta ctgtgtggtc ttgtgtgttt 7800
gattcaattc tacttgtgct ttttcatgcc ctattttatg tacgatatcg tgagtagttt 7860
tgagggttat gattttaagt atatagaaaa tggtcagttg aagaattttg aagcgccact 7920
taaatgcgtc agaaacgttt ttgaaaactt tgaggactgg cattatgcta agtttggctt 7980
cacaccttta aacaagcaaa gctgtcctat tgtagttgga gtttctgaaa ttgttaatac 8040
tgtcgctggc attccatcta atgtgtatct tgttggtaaa actttaattt ttacactaca 8100
agctgctttt ggtaatgctg gtgtttgtta tgacattttt ggagtcacaa cacctgaaaa 8160
gtgcattttt acttctgctt gtactagatt agaaggtttg ggtggtaaca atgtttattg 8220
ttataacaca gcgcttatgg aaggttcttt gccttacagt tcaatacaag ctaatgcata 8280
ttataaatat gacaatggca attttattaa gttgccagaa gttattgcac aaggctttgg 8340
ttttagaaca gtgcgtacta ttgccaccaa atactgccgc gtaggtgaat gtgttgaatc 8400
caatgcaggt gtgtgttttg gctttgacaa gtggtttgtt aacgatggac gtgttgccaa 8460
tggttacgtt tgtggtactg gtttgtggaa ccttgtattt aacatacttt ccatgttttc 8520
atcttcattc tctgttgctg caatgtcagg tcaaatttta cttaattgtg cattaggtgc 8580
ttttgctatt ttttgttgtt ttcttgtgac aaagtttaga cgcatgtttg gtgacctttc 8640
tgtaggtgtt tgcactgttg ttgtggctgt tttgcttaac aatgtctctt acattgtaac 8700
tcagaattta gtaacaatga ttgcttatgc catattgtat ttctttgcta ctagaagctt 8760
acgctatgca tggatttggt gtgctgcata tttaattgcg tatatttctt ttgctccatg 8820
gtggttgtgt gcttggtact ttcttgctat gttgacaggt ttgttaccta gtttgctgaa 8880
gcttaaagtt tcgacaaatc ttttcgaagg tgacaaattt gtaggtacat ttgaaagtgc 8940
tgctgcagga acatttgtca ttgacatgcg ttcttatgag aaacttgcta atagcatctc 9000
tccagaaaag ttgaaaagtt atgctgctag ctataataga tataagtact atagtggtaa 9060
tgcaaatgaa gctgattacc gttgcgcttg ttatgcctat ttagcaaaag caatgttgga 9120
cttttcgcgt gatcataatg acatcttgta cacacctccg actgtcagtt atggttctac 9180
attacaggct ggtttgcgca aaatggcaca accatctggc tttgtggaga aatgtgttgt 9240
ccgtgtctgc tatggaaaca ctgtgttgaa tgggttgtgg cttggtgata ttgtttattg 9300
cccacgtcat gttatcgcat ctaacacaac ttctgctata gattatgatc acgaatatag 9360
tattatgcgg ttgcataatt tttctataat atctggtaca gcatttcttg gtgttgtagg 9420
tgctactatg catggagtaa ctcttaaaat taaggtttca cagactaaca tgcacacacc 9480
tagacattct tttagaacac taaaatctgg tgaaggtttt aacatcttag catgctatga 9540
tggttgtgct caaggtgttt ttggtgtgaa catgagaact aattggacta tccgtggttc 9600
atttattaat ggtgcgtgtg gttcccctgg ctacaatctt aaaaatggcg aggtggaatt 9660
tgtttatatg catcaaattg aactcggaag tggtagccat gtaggttcta gctttgatgg 9720
tgttatgtat ggtggttttg aagaccaacc taatcttcaa gttgaatctg caaaccagat 9780
gttaacagtt aatgtggttg catttcttta tgctgctata ttgaatggtt gcacatggtg 9840
gcttaaaggt gaaaaattgt ttgtggagca ttataatgag tgggcacagg ctaatggttt 9900
cacagctatg aatggtgaag acgctttttc cattcttgct gctaaaactg gtgtctgtgt 9960
ggaaagatta cttcatgcta ttcaagtttt gaataatggc tttggtggta aacaaatttt 10020
gggttattct agtctcaatg atgagttcag tattaatgaa gttgtcaaac aaatgtttgg 10080
tgttaacctg caaagtggta aaaccactag tatgtttaaa tccataagct tatttgctgg 10140
cttctttgtc atgttctggg ctgaattatt tgtttatacc accactattt gggttaaccc 10200
tggttttctt actccgttta tgattttgct tgttgctttg tcactctgtc ttacatttgt 10260
tgttaaacat aaggttttgt ttttgcaagt gtttttgttg ccttcaatta ttgtggctgc 10320
tattcaaaac tgtgcttggg actaccatgt tacaaaggtg ttggcagaga agtttgatta 10380
taatgtttct gttatgcaaa tggacatcca gggttttgtt aacattttta tttgtctttt 10440
tgttgcactg ttgcatactt ggcgctttgc taaagagcgt tgtacacatt ggtgcactta 10500
tttgttctca ctcattgctg ttttatacac tgcattgtat agttatgact acgttagttt 10560
gctggttatg ctactttgtg caatttctaa tgaatggtat attggtgcta ttatttttag 10620
aatttgtcgt tttggtgttg catttttacc agtggaatac gtgtcttact ttgatggtgt 10680
taaaactgtg ctgttgtttt acatgttgtt aggctttgtt agctgtatgt actatggttt 10740
gttgtactgg attaacaggt tctgtaagtg cacattaggt gtttatgatt tctgtgttag 10800
tccagccgaa tttaagtata tggttgctaa tggtttgaat gcaccaaatg gcccttttga 10860
tgcgctcttt ctgtctttta aactaatggg tattggcggt cctagaacca ttaaagtttc 10920
tactgtacag tctaaattga ctgatcttaa gtgcacaaac gtcgttctaa tgggcatttt 10980
gtctaacatg aacatagctt ctaattcaaa ggagtgggca tattgtgttg aaatgcacaa 11040
taaaataaac ttgtgtgacg accctgaaac tgctcaagag ttattgctgg cgttgttggc 11100
ctttttcttg tctaagcata gtgattttgg tcttggtgat cttgtcgatt cttattttga 11160
gaacgactcc attttgcaaa gtgttgcatc ttcttttgtt ggtatgccat cttttgttgc 11220
atatgaaaca gcaagacaag agtatgaaaa tgctgttgca aatggttcct caccacaaat 11280
aatcaaacaa ttgaagaagg ctatgaatgt tgcaaaagct gagtttgaca gggaatcatc 11340
tgttcaaaag aaaattaaca gaatggctga acaagctgct gcagctatgt acaaagaagc 11400
acgtgctgtt aatagaaaat caaaagttgt tagtgccatg catagtttac tctttggcat 11460
gctccgacgt ttggacatgt ctagtgttga cactatcctt aatatggcac gtaatggtgt 11520
tgtccctctt tccgttatcc ctgctacttc tgcagccagg ctcgtcgtcg tagtaccaga 11580
tcatgattca tttgtgaaaa tgatggtaga tggttttgtg cactacgctg gtgttgtttg 11640
gacattacag gaagttaagg ataatgatgg taagaatgtg catcttaaag atgttacaaa 11700
ggaaaaccag gaaatacttg tttggcctct gattttgact tgtgaacgtg tcgttaaatt 11760
gcagaacaat gaaataatgc cgggcaagat gaaggtcaag gccaccaaag gtgaaggtga 11820
tggaggcatt actagtgaag gtaatgctct atacaacaat gaaggtggac gtgcattcat 11880
gtatgcatat gtgactacga agcctggcat gaagtatgtt aaatgggaac atgactctgg 11940
tgtggttaca gttgaattgg aaccaccttg cagatttgtt atagacacac ctactggacc 12000
ccaaattaag tatctttatt ttgttaagaa tcttaacaat ttaaggagag gtgctgtttt 12060
gggttacatt ggtgccactg tgagattgca agctggcaaa cagactgagt ttgtttcaaa 12120
ctcccattta ttaacacatt gttcttttgc tgttgaccca gctgcagcct atcttgatgc 12180
tgttaaacaa ggcgcaaaac ctgttggcaa ttgtgtaaag atgttgacta atggttctgg 12240
tagcggtcag gctattactt gtaccattga ttccaacact acgcaggaca catatggtgg 12300
cgcgtctgtt tgtatttatt gcagagcaca tgttgcacat ccaaccatgg acggtttttg 12360
tcagtacaaa ggcaagtggg tacaagtgcc tataggtaca aatgacccta taagattttg 12420
tcttgaaaat actgtttgta aagtttgtgg ttgttggctt aatcatggct gtacatgtga 12480
ccggactgct atccaaagtt ttgataacag ttatttaaac gagtccgggg ctctagtgcc 12540
gctcgactag agccctgtaa tggtacagac atagattact gtgtccgtgc atttgacgtt 12600
tacaataaag atgcgtcttt tatcggaaaa aatctgaagt ccaattgtgt gcgcttcaag 12660
aatgtagata aggatgacgc gttctatatt gttaaacgtt gcattaagtc agttatggac 12720
cacgagcagt ccatgtataa cttacttaaa ggctgtaatg ctgttgctaa gcatgatttc 12780
tttacttggc atgagggcag aaccatttat ggtaatgtta gtagacagga tcttactaaa 12840
tacaccatga tggatttgtg cttcgctctg cgtaactttg atgaaaaaga ctgtgaagtt 12900
tttaaggaga tattggttct tactggttgt tgtagtactg attactttga aatgaagaat 12960
tggtttgacc ccatagaaaa tgaggacata caccgtgtgt atgctgcttt aggtaaggta 13020
gttgcaaatg caatgcttaa gtgtgttgct ttttgcgacg aaatggtgct caaaggagtt 13080
gttggtgttt tgaccttaga caaccaagat cttaatggga atttctatga cttcggtgac 13140
tttgtattgt gtcctcctgg aatgggaata ccctactgca cgtcatacta ttcttatatg 13200
atgcctgtta tgggtatgac taattgttta gctagtgagt gctttatgaa aagtgacatc 13260
tttggtcaag acttcaaaac ttttgatttg ttgaaatatg atttcacaga acataaggag 13320
gttttgttta acaagtactt taagtattgg ggacaggatt atcatcctga ttgtgttgat 13380
tgccatgacg agatgtgtat tttgcattgt tcaaatttta acacactctt cgcaaccaca 13440
attccaaaca cggcttttgg acctctatgc agaaaagtgt ttattgatgg tgtacccgta 13500
gttgctactg ctggttacca ctttaaacaa ttaggacttg tgtggaacaa agatgttaac 13560
actcattcta ccagacttac tattactgaa ctcttacagt ttgtgacaga tccaacgctt 13620
atagttgcgt catcgcctgc cttggtggat aaacgcactg tttgtttttc tgtcgctgct 13680
ttgagtacag gattaacatc ccaaacagta aaacctggcc attttaataa ggagttttat 13740
gacttcttac gttctcaggg gtttttcgat gagggttcag aattaacatt gaagcatttc 13800
ttttttacac aaaagggtga tgctgcaatt aaagattttg attattatcg ttacaacaga 13860
cctactatgc tggatattgg acaagctcgc gtagcatatc aagtggcagc tcgctatttt 13920
gactgttacg agggtggctg tattacatct agagaggttg ttgttacaaa ccttaataaa 13980
agcgctggtt ggccccttaa taagtttggt aaagctggtt tatattatga gtctattagt 14040
tatgaggaac aagatgctat tttttcatta acaaagcgta atattctccc tactatgact 14100
cagttaaatc ttaaatacgc catatctggt aaggaacgcg cacgtacagt gggtggcgtc 14160
tctttattag ctactatgac tacaagacag tttcatcaga aatgtctgaa atccatagta 14220
gctaccagaa atgccaccgt tgttatcggc actaccaagt tttatggcgg gtgggataat 14280
atgttaaaga acctgatggc cgatgttgat gatcctaaat tgatgggatg ggactatcct 14340
aagtgtgata gagctatgcc ctcaatgatt cgtatgttgt cggctatgat cttaggttct 14400
aagcatgtca catgttgtac ggctagtgat aaattttata gacttagtaa tgagcttgct 14460
caagttttga ccgaggttgt ttattcaaat ggtgggtttt attttaaacc tggtggtaca 14520
acttctggtg atgcaactac agcctacgcc aattctgtct ttaatatatt tcaggctgta 14580
agttctaaca ttaattgcgt tttgagcgtt aactcgtcaa attgcaataa ttttaatgtt 14640
aagaagttac agagacaact ttatgataat tgctatagaa atagtaatgt tgatgaatct 14700
tttgtggatg acttttatgg ttatttgcaa aagcattttt ctatgatgat tctttctgat 14760
gatagtgttg tgtgctataa taaaacttat gctggacttg gttacattgc tgatattagt 14820
gcttttaaag ccactttgta ttatcagaat ggtgtgttta tgagtacagc taagtgttgg 14880
actgaggaag atctttctat aggacctcat gaattttgct cacagcacac tatgcagatt 14940
gtagatgaaa atggtaagta ttatctacca tatccagatc ctagccgtat tatttctgct 15000
ggtgtttttg tggatgacat cactaagact gatgctgtca ttcttttgga acgctatgtt 15060
tctctggcta tagatgccta cccattgtct aagcatccta aacctgagta caggaaggtg 15120
ttttacgcat tgttagactg ggtcaaacat ctcaacaaga ctcttaacga aggtgttttg 15180
gagtcttttt ctgttacact tttagatgaa catgagtcta agttttggga tgaaagcttt 15240
tatgctagta tgtatgagaa gtctacagta ttacaagctg ctggtctttg tgtagtatgt 15300
ggttctcaaa cagttctaag atgcggtgat tgtttacgca gaccgatgtt gtgcactaag 15360
tgcgcctatg atcatgtgtt tggcactgat cataagttca ttttagctat tacaccatat 15420
gtgtgtaaca catctggctg caatgtaaat gacgttacaa aactgtatct tggaggtttg 15480
aattattact gtgtagacca caaaccacat ctttcattcc cactgtgttc agctggtaat 15540
gtctttggtt tgtacaaaag ttctgctttg ggttccatgg acattgatgt ctttaacaaa 15600
ctttctacct ctgattggtc tgacattcgc gactacaagc ttgctaatga tgcaaaagag 15660
tcactaaggt tgtttgcagc tgaaacggtc aaggctaaag aggaaagtgt taagtcatca 15720
tacgcttatg ctaccctaaa ggagattgta ggtcctaagg aacttttgct cttatgggaa 15780
agtggaaaag ccaaaccacc gttaaaccgt aattctgttt ttacatgctt ccaaattaca 15840
aaagactcca agtttcaagt tggtgagttt gtgtttgaga aagtagatta cggttctgat 15900
acggttactt acaaatccac tgctactact aagttagtac caggtatgtt gtttattttg 15960
acttctcata atgttgctcc acttagagcg ccaacaatgg caaaccagga gaaatattct 16020
accatttaca agttgcaccc atcatttaat gttagtgatg cttatgcaaa tcttgtacct 16080
tattaccaac ttattggcaa acagcgtata accacaatac agggtcctcc tggtagtgga 16140
aaatcgcatt gttctattgg tattggtgtg tattaccctg gagcgaggat cgtgttcacc 16200
gcttgttctc acgctgctgt tgattcgctc tgtgcaaaag ctgtcacagc ctatagtgtt 16260
gataagtgta cacgtattat tcctgcacgt gccagagttg agtgttatag tggttttaaa 16320
cctaacaata atagtgcaca atacgtgttt agtactgtta atgcgttacc tgaagttaat 16380
gcagacattg ttgtcgtgga tgaggtgtct atgtgcacta actatgactt gtctgtgatt 16440
aaccagcgta tatcatataa acacattgta tatgttggtg atcctcaaca gcttccagct 16500
cctagagttc ttatctctaa aggtgttatg gaaccaattg actataatgt tgtgacacaa 16560
cgtatgtgtg ctataggacc cgatgtcttt ttacacaagt gttacagatg tcctgctgaa 16620
atagttaaca ctgtttcaga gcttgtttat gaaaacaagt ttgtacctgt caaagaagct 16680
agtaagcagt gcttcaaaat ctttgaacgc ggtagtgttc aggtagacaa tggctccagt 16740
ataaataggc gtcaacttga tgttgttaag cgatttatac ataaaaactc cacatggagc 16800
aaggctgtgt ttatctcacc ttacaatagt caaaattatg tagctgccag gcttttaggc 16860
ttacaaactc agacagtgga ttctgctcaa ggtagtgaat atgactatgt tatattcgca 16920
cagacatcag atactgctca tgcctgtaat gccaatcgtt ttaacgttgc cattactaga 16980
gcaaagaaag gtattttctg tattatgtct gacagaactt tgtttgatgc acttaagttc 17040
tttgaaatca ctatgacaga tttacagtct gaaagtagtt gtggtttgtt taaggattgt 17100
gcacgtaacc ctattgattt accaccaagt catgccacta cttatttgtc attgtctgat 17160
agatttaaga ctagtggtga cttggctgtt caaataggta acaacaatgt ttgtacctat 17220
gaacatgtga tttcatatat gggtttcagg tttgatgtta gcatgcctgg tagtcatagt 17280
ttgttctgta ctagagactt tgccatgcgt catgtcagag gttggttagg aatggatgtg 17340
gaaggtgcac atgtcacagg tgacaatgtt ggcactaatg tacctctaca agttggtttt 17400
tccaatggtg ttgattttgt agctcaacct gaaggttgtg ttctaacaaa cactggcagt 17460
gttgtaaaac ctgttcgtgc tcgtgcacca cctggagaac aattcactca cattgtacct 17520
ctgttacgca agggacaacc ttggagtgtg ttgagaaaac gtattgttca aatgatagca 17580
gattttcttg ctggctcatc tgatgtactg gtgtttgtac tttgggctgg cggtttagag 17640
ttgaccacta tgcgttattt tgttaagatt ggagctgtta aacattgcca atgtggtact 17700
gttgcaacat gctacaattc tgttagtaat gactattgtt gctttaaaca tgcattgggc 17760
tgtgactatg tttataatcc atatgtcata gatattcaac aatggggtta tgttggttca 17820
ctctccacta atcaccatgc aatttgtaat gttcatagaa atgagcatgt tgcttctggt 17880
gatgctatta tgactagatg tttggctgtg tatgactgct ttgttaagaa tgtggattgg 17940
tcaattacct accctatgat agctaatgaa aatgccataa acaagggcgg tcgcactgtg 18000
cagagtcata ttatgcgtgc tgctattaaa ttgtacaacc ctaaagcaat ccatgacatt 18060
ggtaatccta agggtattcg ttgtgctgta actgatgcca agtggtattg ttatgacaag 18120
aaccctatta attctaatgt gaaaacattg gagtatgatt acatgacaca tggccaaatg 18180
gatggcttgt gtttgttttg gaattgtaat gtggatatgt accctgaatt ctcaattgtt 18240
tgcaggtttg acacacgtac acgatctaca ttgaaccttg aaggtgtaaa tggtgggtca 18300
ttgtatgtca ataatcatgc atttcacact cctgcttatg ataaacgtgc tatggctaaa 18360
ttgaaaccag caccgttttt ctactatgac gacggttcat gtgaggttgt tcacgatcaa 18420
gttaactatg ttcctttgag agccactaat tgcattacca agtgtaatat tggtggtgct 18480
gtatgttcta agcacgctaa tctctataga gcatatgttg agtcatataa catttttact 18540
caagctggtt ttaatatttg ggttcctacc acgtttgatt gttataattt gtggcagaca 18600
ttcacagagg tcaatttaca aggtttagag aacattgctt ttaacgttgt taataaaggt 18660
tcatttgttg gtgctgatgg tgaattacca gtagccatta gtggtgataa agtgttcgta 18720
cgtgatggta acactgataa tttagtcttt gttaacaaaa catcactgcc tacaaacata 18780
gcatttgaac tttttgctaa gaggaaggtt ggtttaacac cacctctcag tattctcaaa 18840
aaccttggtg ttgtcgccac atataagttt gtcttgtggg attatgaagc tgagcgtccc 18900
ttgacaagct ttactaagtc tgtttgtggt tatacagact ttgcagagga tgtttgtact 18960
tgttacgata atagtataca aggttcatac gaacgtttta ctctgtcaac taatgctgtg 19020
ttattctctg ctactgctgt gaaaacaggt ggtaagagtt tgccggctat taaattgaat 19080
tttggaatgc ttaatggtaa tgcaattgct actgtcaaat cagaagatgg taacataaaa 19140
aatattaact ggtttgttta cgtacgcaaa gatggcaaac ctgttgatca ttatgatggt 19200
ttttataccc aaggtcgtaa tttacaagac tttttgcctc gcagcacaat ggaagaagac 19260
tttttgaaca tggatatagg cgtgtttatt caaaagtatg gtctagagga tttcaacttc 19320
gagcacgttg tgtatggtga tgtttcaaaa actactctag gcggtttaca cttgttgatt 19380
tcacaagtac gtctgagtaa aatgggcatc ttaaaggcag aggagtttgt ggcagcatct 19440
gacataacac tcaaatgttg tactgtgact tatcttaatg atcctagttc taagactgtt 19500
tgtacttaca tggatttgtt gttggatgat tttgtttctg tattgaagtc tttggatttg 19560
actgttgtat ccaaggttca tgaggtcata attgacaaca aaccatggag atggatgcta 19620
tggtgtaaag ataatgccgt tgctacattc tatcctcagt tgcagagtgc agaatggaaa 19680
tgcgggtatt ctatgcctgg tatttataag acacaacgta tgtgcttaga accatgtaat 19740
ttgtataatt atggtgcagg tttgaagttg cccagtggca ttatgttcaa tgttgttaaa 19800
tacactcaat tgtgtcaata ttttaacagt accacgttat gtgttcctca taatatgaga 19860
gtgttacact tgggtgctgg ctctgattat ggtgttgcac caggaactgc tgttcttaaa 19920
aggtggttgc cgcacgacgc aattgttgtt gacaacgatg ttgttgacta tgtgagtgac 19980
gctgatttta gtgttactgg tgattgtgca accgtttatt tggaagacaa gtttgacttg 20040
ttaatctctg atatgtacga tggtaggaca aaggcaattg atggtgaaaa tgtttcgaaa 20100
gaaggatttt tcacttacat caatggtttc atttgtgaaa aacttgccat cggaggttcg 20160
attgctatta aagtaacaga gtatagctgg aataagaaat tgtatgaact tgtacaaaga 20220
ttttcttttt ggactatgtt ttgcacttct gttaatacgt catcatcaga agcctttgtt 20280
gtcggaatta actatcttgg tgatttcgca caaggacctt ttatagatgg taacataata 20340
cacgcaaatt atgtattttg gcgtaactcc actgttatga gtttgtccta caactctgtt 20400
ttagacctga gtaaatttaa ttgcaaacac aaagcgactg ttgttgtgca attaaaggat 20460
agtgatatta atgaaatggt gcttagtctt gttaggagtg gtaagttgct tgtaaggggt 20520
aatggcaagt gtttgagttt tagtaatcat ttagtctcaa ctaaataaaa tgtttgtttt 20580
gcttgttgca tatgccttgt tgcatattgc tggttgtcaa actacaaatg ggctgaacac 20640
tagttactct gtttgcaacg gctgtgttgg ttattcagaa aatgtatttg ctgttgagag 20700
tggtggttat ataccctccg actttgcatt caataattgg ttccttctaa ctaatacctc 20760
atctgttgta gatggtgttg tgaggagttt tcagcctttg ttgcttaatt gcttatggtc 20820
tgtttctggc ttgcggttta ctactggttt tgtctatttt aatggtactg ggagaggtga 20880
ttgtaaaggt ttttcctcag atgttttgtc tgatgtcata cgttacaacc tcaattttga 20940
agaaaacctt agacgtggaa ccattttgtt taaaacatct tatggtgttg ttgtgtttta 21000
ttgtaccaac aacactttag tttcaggtga tgctcacata ccatttggta cagttttggg 21060
caatttttat tgctttgtaa atactactat tggcaatgaa actacgtctg cttttgtggg 21120
tgcactacct aagacagttc gtgagtttgt tatttcacgc acaggacatt tttatattaa 21180
tggctatcgc tatttcactt taggtaatgt agaagccgtt aatttcaatg tcactactgc 21240
agaaaccact gatttttgta ctgttgcgtt agcttcttat gctgacgttt tggttaatgt 21300
gtcacaaacc tctattgcta atataattta ttgcaactct gttattaaca gactgagatg 21360
tgaccagttg tcctttgatg taccagatgg tttttattct acaagcccta ttcaatccgt 21420
tgagctacct gtgtctattg tgtcgctacc tgtttatcat aaacatacgt ttattgtgtt 21480
gtacgttgac ttcaaacctc agagtggcgg tggcaagtgc tttaactgtt atcctgctgg 21540
tgttaatatt acactggcca attttaatga aactaaaggg cctttgtgtg ttgacacatc 21600
acacttcact accaaatacg ttgctgttta tgccaatgtt ggtaggtgga gtgctagtat 21660
taacacggga aattgccctt tttcttttgg caaagttaat aactttgtta aatttggcag 21720
tgtatgtttt tcgctaaagg atatacccgg tggttgcgca atgcctatag tggctaattg 21780
ggcttatagt aagtactata ctataggctc attgtatgtt tcttggagtg atggtgatgg 21840
aattactggc gtcccacaac ctgttgaggg tgttagttcc tttatgaatg ttacattgga 21900
caaatgtact aaatataata tttatgatgt atctggtgtg ggtgttattc gcgttagcaa 21960
tgacaccttt cttaatggaa ttacgtacac atcaacttca ggtaaccttc tgggttttaa 22020
agatgttact aagggcacca tctactctat cactccttgt aacccaccag atcagcttgt 22080
tgtttatcag caagctgttg ttggtgctat gttgtctgaa aattttacta gttacggctt 22140
ttctaatgtt gtagaactgc cgaaattttt ctatgcgtcc aatggcactt ataattgcac 22200
agacgctgtt ttaacttatt ctagttttgg cgtttgtgca gatggttcta taattgctgt 22260
tcaaccacgt aatgtttcat atgatagtgt ttcagctatc gtcacagcta atttgtctat 22320
accttccaat tggaccactt cggtccaggt tgagtattta caaattacaa gtacacctat 22380
cgtagttgat tgctccactt atgtttgcaa tggtaatgtg cgctgtgttg aattgcttaa 22440
gcagtatact tctgcttgta aaactattga agacgcctta agaaatagcg ccaggctgga 22500
gtctgcagat gttagtgaga tgctcacttt tgacaagaaa gcgtttacac ttgctaatgt 22560
tagtagtttt ggtgactaca accttagcag cgtcatacct agcttgccca caagtggtag 22620
tagagtggct ggtcgcagtg ccatagaaga catacttttt agcaaacttg ttacttctgg 22680
acttggcact gtggacgcag actacaaaaa gtgcactaag ggtctttcca ttgctgactt 22740
ggcttgtgct caatattata atggcattat ggttttgcct ggcgtcgctg atgctgaacg 22800
aatggccatg tatacaggtt ctttaattgg tggaattgct ttaggaggtc taacatcagc 22860
cgtttcaata ccattttcat tagcaattca ggcacgttta aattatgttg cattgcagac 22920
tgatgtttta caagaaaatc agaaaattct tgctgcatct tttaacaaag caatgaccaa 22980
catagtagat gcctttactg gtgttaatga tgctattaca caaacttcac aagccctaca 23040
aacagttgct actgcactta acaagatcca ggatgttgtt aatcaacaag gcaactcatt 23100
gaaccattta acttctcagt tgaggcagaa ttttcaagct atctctagct ctattcaggc 23160
tatctatgac agacttgaca ctattcaggc tgatcaacaa gtagataggc tgattactgg 23220
tagattggct gctttgaatg tattcgtttc tcatacattg actaagtaca ctgaagttcg 23280
tgcttccaga cagcttgcac aacaaaaagt gaatgagtgt gtcaaatccc agtctaagcg 23340
ttatggcttc tgtggaaatg gcactcacat tttctcaatt gttaatgctg ctcctgaggg 23400
gcttgttttt ctccacactg tcttgttgcc gacacaatat aaggatgttg aagcgtggtc 23460
tgggttgtgc gttgatggta caaacggtta tgtgttgcga caacctaatc ttgctcttta 23520
caaagaaggc aattattata gaatcacatc tcgcataatg tttgaaccac gtattcctac 23580
catggcagat tttgttcaaa ttgaaaattg caatgtcaca tttgttaaca tttctcgctc 23640
tgagttgcaa accattgtgc cagagtatat tgatgttaat aagacgctgc aagaattaag 23700
ttacaaattg ccaaattaca ctgttccaga cctagttgtc gaacagtaca accagactat 23760
tttgaatttg accagtgaaa ttagcaccct tgaaaataaa tctgcggagc ttaattacac 23820
tgttcaaaaa ttgcaaactc tgattgacaa cataaatagc acattagtcg acttaaagtg 23880
gctcaaccgg gttgagactt acatcaagtg gccgtggtgg gtgtggttgt gcatttcagt 23940
cgtgctcatc tttgtggtga gtatgttgct attatgttgt tgttctactg gttgctgtgg 24000
cttctttagt tgttttgcat cttctattag aggttgttgt gaatcaacta aacttcctta 24060
ttacgacgtt gaaaagatcc acatacagta atggctctag gtttgttcac attgcaactt 24120
gtgtctgctg ttaatcaatc gcttagcaat gcgaaagtta gtgctgaagt ttcacgacag 24180
gttatccaag acgtgaaaga tggcactgtt accttcaact tgctagcgta tacactaatg 24240
agcctctttg ttgtgtattt tgctttattt aaagcaagat cacaccgtgg cagagctgct 24300
cttatagtgt ttaaaattct aatccttttc gtttatgtgc cattgctgta ttggtctcaa 24360
gcatatattt acgcaacttt gattgctgta attttgcttg gaagattttt ccatacagct 24420
tggcactgct ggctctacaa gacatgggat ttcattgtct tcaatgtaac cacactttgc 24480
tatgcaaggt aagtgttggt ttcttgaaaa taaggctctg aaaccattcg tttgttttta 24540
cggaggggat caattccttt acataggcga cagaattgtt tcttatttct caactaacga 24600
cttgtacgtt gctcttagag gacgtattga taaagacctc agcctttcta gaaaggttga 24660
gttatataac ggtgaatgtg tatacttgtt ttgtgaacac ccagctgttg gaatagtcaa 24720
cacagatttc aaattagaaa tccactaaga tgttccttaa gctagtggat gatcatgctt 24780
tggttgttaa tgtactactc tggtgtgtgg tgcttatagt gatactacta gtgtgtatta 24840
caataattaa actaattaag ctttgtttca cttgccatat gttttgtaat agaacagttt 24900
atggccccat taaaaatgtg taccacattt accaatcata tatgcacata gaccctttcc 24960
ctaaacgagt tattgatttc taaactaaac gacaatgtca aatgacaatt gtacgggtga 25020
cattgtcacc catttgaaga attggaattt tggttggaat gttattctaa ccatattcat 25080
tgttattctt cagtttggac actataaata ctccagattg ttttatggtt tgaagatgct 25140
tgtactgtgg cttctttggc cactcgtact tgctttgtca atctttgaca cctgggctaa 25200
ttgggattct aattgggcct ttgttgcatt tagctttttt atggccgtat caacactcgt 25260
tatgtgggtg atgtacttcg caaacagttt cagacttttc cgacgtgctc gaactttttg 25320
ggcatggaat cctgaggtta atgcaatcac tgtcacaacc gtgttgggac agacatacta 25380
tcaacccatt caacaagctc caacaggcat tactgtgacc ttgctgagcg gcgtgcttta 25440
cgttgacgga catagattgg cttcaggtgt tcaggttcat aacctacctg aatacatgac 25500
agttgccgtg ccgagcacta ctataattta tagtagagtc ggaaggtccg taaattcaca 25560
aaatagcaca ggctgggttt tctacgtacg agtaaaacac ggtgattttt ctgcagtgag 25620
ctctcccatg agcaacatga cagaaaacga aagattgctt cattttttct aaactgaacg 25680
aaaagatggc tacagtcaaa tgggctgatg catctgaacc acaacgtggt cgtcagggta 25740
gaatacctta ttctctttat agccctttgc ttgttgatag tgaacaacct tggaaggtga 25800
tacctcgtaa tttggtaccc atcaacaaga aagacaaaaa taagcttata ggctattgga 25860
atgttcaaaa acgtttcaga actagaaagg gcaaacgggt ggatttgtca cccaagctgc 25920
atttttatta tcttggcaca ggaccccata aagatgcaaa atttagagag cgtgttgaag 25980
gtgtcgtctg ggttgctgtt gatggtgcta aaactgaacc tacaggttac ggtgttaggc 26040
gcaagaattc agaaccagag ataccacact tcaatcaaaa gctcccaaat ggtgttactg 26100
ttgttgaaga acctgactcc cgtgctcctt cccggtctca gtcgaggtcg cagagtcgcg 26160
gtcgtggtga atccaaacct caatctcgga atccttcaag tgacagaaac cataacagtc 26220
aggatgacat catgaaggca gttgctgcgg ctcttaaatc tttaggtttt gacaagcctc 26280
aggaaaaaga taaaaagtca gcgaaaacgg gtactcctaa gccttctcgt aatcagagtc 26340
ctgcttcttc tcaaacttct gccaagagtc ttgctcgttc tcagagttct gaaacaaaag 26400
aacaaaagca tgaaatgcaa aagccacggt ggaaaagaca gcctaatgat gatgtgacat 26460
ctaatgtcac acaatgtttt ggccccagag accttgacca caactttgga agtgcaggtg 26520
ttgtggccaa tggtgttaaa gctaaaggct atccacaatt tgctgagctt gtgccgtcaa 26580
cagctgctat gctgtttgat agtcacattg tttccaaaga gtcaggcaac actgtggtct 26640
tgactttcac tactagagtg actgtgccca aagaccatcc acacttgggt aagtttcttg 26700
aggagttaaa tgcattcact agagaaatgc aacaacatcc tcttcttaac cctagtgcac 26760
tagaattcaa cccatctcaa acttcacctg caactgctga accagtgcgt gatgaagttt 26820
ctattgaaac tgacataatt gatgaagtaa actaaacatg ccactgtgtt gtttgaaatt 26880
caggctttag ttggaatttt gcttttgttc tttcttttat tatctttctt ttgcctgttt 26940
ttagagagat ttggcgcctt ggtgccgtag atgaatacat tgcttttctc tgatctatgt 27000
atgatggtac gatcagagct gcttttaatt aacatgatcc cttgctttgg cttgacaagg 27060
atctagtctt atacacaatg gtaagccagt ggtagtaaag gtataagaaa tttgctacta 27120
tgttactgaa cctaggtgaa cgctagtata actcattaca aatgtgctgg agtaatcaaa 27180
gatcgcattg acgagccaac aatggaagag ccagtcattt gtcttgagac ctatctagtt 27240
agtaactgct aatggaacgg tttcgatatg gatacacaaa aaaaaaaaaa aaaaaaaaaa 27300
aaaaaaaaaa aaaaaaa 27317
<210> 10
<211> 7152
<212> DNA
<213> Human rhinovirus
<400> 10
ttaaaactgg gagtgggttg ttcccactca ctccacccat gcggtgttgt actctgttat 60
tacggtaact ttgtacgcca gtttttccca cccttcccca taatgtaact tagaagtttg 120
tacaatatga ccaataggtg acaatcatcc agactgtcaa aggtcaagca cttctgtttc 180
cccggtcaat gaggatatgc tttacccaag gcaaaaacct tagagatcgt tatccccaca 240
ctgcctacac agagcccagt accatttttg atataattgg gttggtcgct ccctgcaaac 300
ccagcagtag acctggcaga tgaggctgga cattccccac tggcgacagt ggtccagcct 360
gcgtggctgc ctgctcaccc ttcttgggtg agaagcctaa ttattgacaa ggtgtgaaga 420
gccgcgtgtg ctcagtgtgc ttcctccggc ccctgaatgt ggctaacctt aaccctgcag 480
ccgttgccca taatccaatg ggtttgcggt cgtaatgcgt aagtgcggga tgggaccaac 540
tactttgggt gtccgtgttt cctgtttttc ttttgattgc attttatggt gacaatttat 600
agtgtataga ttgtcatcat gggtgcacaa gtatctagac aaaatgttgg gacacactcc 660
acacaaaatt cagtgagcaa tggatctagc ttaaattatt tcaacatcaa ttattttaaa 720
gacgcagctt caagtggtgc ttctagattg gatttttctc aagaccctag taaatttact 780
gatcctgtta aagatgtgtt agaaaagggt attccaacac ttcaatcacc aacagttgaa 840
gcttgtggtt attcagacag actaatacag ataacccgag gagattccac tataacatcc 900
caagatactg caaatgcagt tgttgcttat ggtgtgtggc catcatactt gactccagat 960
gatgcgactg ctattgacaa acccacacaa cctgatacat catccaacag attctacacc 1020
ttggacagtc gttcttggac atctgcctca tctggatggt ggtggaaatt gcctgatgcc 1080
cttaaaaaca tgggtatatt tggtgaaaat atgttttacc attttctagg gagatctgga 1140
tacacaatac atgtacaatg taattctagc aagtttcatc agggtttatt aatagttgcc 1200
gccatcccag aacatcaatt ggcatctgca acaagtggaa atgtatcagt cgggtacaat 1260
cacacccacc caggtgagca aggtagagaa gtagtaccat cacggacatc tagtgataat 1320
aaaagaccta gtgatgacag ttggttaaat tttgatggaa cattacttgg taacttacct 1380
atttatcccc accaatacat taatctaagg actaacaatt cagctaccct tattttacct 1440
tatgtcaatg ctgtaccaat ggactctatg cttagacata ataattggag cttggttata 1500
atcccaatat gccctcttca ggtccaacct ggggggacac aatccatacc tataacagta 1560
tcaattagcc ctatgttttc agaattttca gggccaagaa gtaaggttgt gtttagtacc 1620
actcagggtt taccagttat gttaacacct ggatctgggc aattcttaac aactgatgat 1680
actcaatccc catcagcgtt tccatacttc cacccgacca aggaaatatt tatacctgga 1740
caagttagga atttaattga aatgtgccaa gttgacacac tcattcctgt taacaataca 1800
caggaaaatg taagatctgt gaatatgtac actgttgatt tacgcacaca agttgattta 1860
gctaaagaag tcttttctat accagtagat attgcctcac aacctttagc cactactctc 1920
ataggagaac ttgcaagcta ttacacacac tggactggta gtctgcgctt tagctttatg 1980
ttttgtggtt ctgctagctc tactttgaaa ctattaattg catacactcc tcctggtgtt 2040
ggaaaaccta aatccaggag agaagccatg cttggtacac atttagtgtg ggatgtgggg 2100
ttgcagtcca ccgcctcact agttgtacca tgggttagtg ctagccattt tagattcact 2160
acacctgaca catattcctc agctggttat attacatgct ggtaccagac caactttgta 2220
gtacctgata gtactccaga taacgccaaa atggtgtgca tggtttctgc atgcaaagat 2280
ttttgcttaa gattagccag agatactaac ctacacacac aagaaggagt actcacacaa 2340
aacccagttg aaaattatat agatagtgta ttaaatgaag ttcttgtggt gccaaatatc 2400
caacctagca catctgtgtc aagtcatgca gcgcctgcat tggatgctgc ggaaaccgga 2460
cacaccagct ctgttcaacc tgaagatatg attgaaacta gatatgttat aactgatcaa 2520
acaagggatg aaacaagtat tgagagtttc ttaggtaggt cagggtgtat cgctatgata 2580
gaatttaata caagtagtga taaaactgaa catgataaaa ttggtaaagg attcaaaaca 2640
tggaaggtta gtcttcaaga aatggcacaa atcagaagaa aatatgaatt attcacatat 2700
acaagatttg attcagagat aacaatagtc actgcagccg cagctcaagg aaatgatagt 2760
ggacatatag tattgcaatt tatgtatgta cccccaggag cacctgtccc cgaaaaacgt 2820
gatgattaca catggcaatc aggaacaaat gcatctgtgt tctggcaaga aggacaacca 2880
taccccagat tcacaatccc ttttatgagc attgcatcag cctattacat gttttatgat 2940
ggttatgatg gtgatagtgc agcatcaaaa tacggttctg tagtcactaa tgatatggga 3000
accatatgtg ttagaatagt gacatccaac caaaaacacg attcaaatat tgtgtgccgc 3060
atttaccaca aggccaaaca tataaaagca tggtgtcctc gcccaccaag ggctgttgcc 3120
tatcaacaca cacactcaac caattacata ccatccaatg gtgaggccac aactcagatt 3180
aaaaccagac ctgatgtttt taccgttaca aacgtcggac catctagtat gtttgtacat 3240
gtgggtaact taatctatag aaatcttcat ctctttaatt ctgatcttga tgattctatt 3300
cttgtatcat actccagtga tctaatcata tatcgaacaa acactgaagg taatgatgtg 3360
atccctaatt gtgattgcac tgaatgtaca tattactgcc accacaaaga taggtatttt 3420
cctatcagag ttactgcaca tgattggtat gagattcaag aatcagaata ttacccaaaa 3480
catatccaat ataatctcct gattggagag ggtccttgtg aaccaggaga ttgtggagga 3540
aaactattgt gtaaacatgg tgttataggt atgattacag ctggaggtga aggtcacgtt 3600
gcttttattg acctgagaaa attccagtgt gctgaggagc aagggttatc tgattatgtg 3660
gaacatcttg gtcaagtctt tggtgtaggc ttcgtagaca gcatcaaaca acaggtaaac 3720
tttatcaacc ccactagtaa aattggttca aaagtgatta aatggttgtt gaggatagtt 3780
tcagctatga taataatggt aaggaatagt tctgatccac aaactgtaat tgccactctc 3840
acccttctag gttgttcagg ctcaccatgg aggtttctta aagagaaact ctgtgcgtgg 3900
ctccagctta gctatgtaca taagcagtct gattcatggc tcaagaaatt tactgaagcg 3960
tgtaacgcag cacgtgggct agagtggatt ggacaaaaga tatctaaatt tatagattgg 4020
ataaagagta tgttaccaca ggctcaattg aaaattgatt acctaaccaa attaaaacaa 4080
cttaatctct tagagaaaca aatagaaaca attagacttg cacctgctag tgttcaggag 4140
aaaattttca ttgaaataaa cacccttcat gatttatcct taaaattctt accactgtat 4200
gcatctgaag cacgtagaat taagaattta tatatcaaat gcagtaatgt tattaaaggg 4260
ggaaagagga atgaaccagt tgcagttcta atacatggtt ctcctggtac tggaaaatct 4320
cttgccactt ctgttcttgc tcgaatgcta actgttgaga ctgatatata ttctttgccc 4380
ccagatccta aatattttga tgggtatgat caacagagtg ttgttatcat ggatgatatc 4440
atgcaaaatc ctagtggtga agacatgact ttgttttgcc aaatggtatc gagtgtccct 4500
ttcatacctc ctatggcaga tcttccagat aaaggaaaac catttacatc caagtttgta 4560
cttgcaagca ctaatcacac tctactaaca ccaccaacag tatcttcatt accagcaatg 4620
gcaagaaggt tttactttga tctagacatt caagttaaga aagagtatct tttagatggc 4680
aaactagata tagcaaaaag ctttcgacca tgtgatgtta atattaaaat aggcaatgct 4740
aagtgctgtc catttatctg tggaaaagct gtagagttta aagatagaaa ttcatgtaca 4800
accttgtctt tatctcaatt gtatagtcat ataaaggaag aagataggag aagaagcagt 4860
gcagcacaag caatggaggc tatatttcaa ggtatagacc tccaatctcc tccacctcca 4920
gccatagctg acctccttag gtctgtgaaa acaccagaga tcattaagta ttgccaagat 4980
aataattgga ttgttccagc agagtgttct attgaaagag atttagggat agcaaatatg 5040
actataggta taatagctaa tgtggtctct atagtaggtg ttatctatat aatttataaa 5100
ttgttctgta cacttcaggg tccatactca ggggaaccta aacccaaaag cagagctcca 5160
gagagaagag tagttactca gggcccagag gaagagtttg gtcgctcact actcaaacat 5220
aattgctgtg ttgtgacaac cgacaaaggc aaattcacag gtcttggcat atatgaccaa 5280
gtcatggtac ttccaacaca ttctgaccca ggctctgaga tcttggtaga tggagtaaaa 5340
gttaaggtct ctgattccta tgatttgcat aaccatgagg gtgttaagct agagatcaca 5400
gttgtgaaat taattagaaa tgagaagttt aaagacatca gaaaatattt accctcacgt 5460
gaagatgact atcctgcttg taaccttgcc ttactagcta atcaagatga gccaacaata 5520
ataagtgttg gtgatgcagt atcttatggt aacatcttat tgagtggtac caatactgca 5580
cgaatgatca agtaccatta cccgacaaaa gctggatatt gtgggggtgt tttgtacaag 5640
gttggctcta ttcttggtat acatgttggt ggcaatggta gagatggatt ttctgcaatg 5700
cttctcaaat cttattttgg tgaaacccag ggtttaatca ctaaagaact tcctgtatct 5760
gtaaagaact taccatccgt acatgtttca tctaaaaccc gactacaacc tagtgttttt 5820
catgatgttt tccctggaac aaaagagcct gcagttctta gtagtaatga tccaagacta 5880
gaaactgact ttgactcagc acttttctcc aaatataaag gtaatcctgc ttgtcaagtg 5940
accccacaca tgaaaattgc tgtagcacat tatgcagcac agttatctac actagacata 6000
aatcctcaac ccctttcatt ggaagagagt gtgtttggta ttgagggatt agaagctttg 6060
gatttaaata ctagtgcagg atttccttat gtttcactgg gaataaagaa gaaagatctt 6120
atagataaaa agaccaaaga catcacaaaa cttaggaaag caattgatga atatggtatt 6180
gatttgccta tggttacttt tctgaaagat gaacttagaa agaaggaaaa aataaaagat 6240
ggaaagacta gagtcataga agctaatagt gtgaatgata ctgtgttatt cagaagtgta 6300
tttggaaatc ttttctctgc tttccacaaa aacccaggta tagtcactgg ttcagcagta 6360
gggtgtgacc ctgaagtatt ctggtcaact atacctctca tgctagatgg agaatgttta 6420
atggcttttg attattcaaa ctatgatggt agcctacatc ccgtttggtt taaatgtctt 6480
agtatgctct tagaagacat aggtttctcc tctcaactta ttaaccagat ctgtaactct 6540
aaacatatat acaaatctaa gtattatgaa gtggaaggag gtatgccatc tggatgtgct 6600
ggtactagta tttttaatac aataatcaac aatattatca ttagaacttt ggtactagat 6660
gcttataaga acatagatct agataaactg aaaatcttag catatgggga tgatgtcatc 6720
ttttcttata attttaaact tgacatggca gttcttgcca aagaaggaga aaaatatgga 6780
ctaacaatca cccctgctga taagtctgat gttttccaag aattgaccta taaaaatgta 6840
acttttctta aaagaggatt cagagctgat gagcgccact ctttccttat acaccctacc 6900
tttcctgtgg ctgagattca tgactccatc agatggacca aaaacccttc atgtatgcag 6960
gaacacgtgc tatctttgtg tcatttaatg tggcataatg gtagacatgc ataccaggaa 7020
ttcattaaag gtatacgcag tgtatctgcc ggtcgggcac tgtatatacc agcttatgaa 7080
gttcttgaac atgaatggta tgaaaaattt tagatataaa actgttaaat atagctagtt 7140
tattagtttt at 7152
<210> 11
<211> 36001
<212> DNA
<213> Human adenovirus
<400> 11
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggtaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacgg gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataattct gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatttgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctaat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttcct gactctgtaa tgttggcggc 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcagcccg ggcacggttg 1020
caggtcttgt cattatcatc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aattatgggc agtgggtgat 1140
agagtggtgg gttcggtgtg gtaatttttt ttttaatttt ttgcagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt ttttaaaagg tcctgtgtct gaacctgagc ctgagcccga 1260
gccagaaccg gaacctgcaa gacctgcccg gcgtcctaaa atggtgcctg ctatcctgag 1320
acgcccgaca tcacctgtgt ccagagaatg caatagtagt acggatagct gtgactccgg 1380
tccttctaac acacctcctg agatacatcc ggtggtcccg ctgtgcccca ttaaaccagt 1440
tgccgtgaga gttggtgggc gtcgccgggc tgtggaatgt atcgaggact tgcttaacga 1500
gcctgggcaa cctttggact tgagctgtaa acgccccagg ccataaggtg taaacctgtg 1560
attgcgtgtg tggttaacgc ctttgtttgc tgaatgagtt gatgtaagtt taataaaaag 1620
ggtgagataa tgtttaactt gcatggcgtg ttaaatgggg cggggcttaa agggtatata 1680
atgcgccgtg ggctaatctt ggttacattt gacctcatgg aggcttggga gtgtttggaa 1740
gatttttctg ctgtgcgtaa cttgctggaa cagagctcta acagtacctc ctggttttgg 1800
aggtttctgt ggggctcctc ccaagcaaag ttagtctgca gaattaagga ggattacaag 1860
tgggaatttg aagagctttt gaaatcctgt ggtgagctgt ttgattcttt gaatctgggt 1920
caccaggcgc ttttccaaga gaaggtcatc aagactttgg atttttccac accggggcgc 1980
gctgcggctg ctgttgcttt tttgagtttt ataaaggata aatggagcga agaaacccat 2040
ctgagcgggg ggtacctgct ggattttctg gccatgcatt tgtggagagc ggtggtgaga 2100
cacaagaatc gcctactact gttgtcttcc gtccgcccgg caataatacc gacggaggag 2160
cagcagcagc aacagcagca gcaggaggaa gccaggcggc ggcggcggca ggagcagagc 2220
ccatggaacc cgagagccgg cctggaccct cgggaatgaa tgttgtacag gtggctgaac 2280
tgtttccaga actgagacgc attttaacaa ttaacgagga tgggcagggg ctaaaggggg 2340
taaagaggga gcggggggct tttgaggcta cagaggaggt taggaattta acttttagct 2400
taatgaccag acaccgtcct gagtgtgtta cttttcagca gattaaggat aattgcgcta 2460
atgagcttga tctgctggcg cagaagtatt ccatagagca gctgaccact tactggctgc 2520
agccagggga tgattttgag gaggctatta gggtatatgc aaaggtggca cttaggccag 2580
attgcaagta caagattagc aaacttgtaa atattaggaa ttgttgctac atttctggga 2640
acggggccga ggtggagata gatacggagg atagggtggc ctttagatgt agcatgataa 2700
atatgtggcc gggggtactt ggcatggacg gggtggttat tatgaatgtg aggtttactg 2760
gccccaattt tagcggtacg gttttcctgg ccaataccaa ccttatccta cacggtgtaa 2820
gcttctatgg gtttaacaat acctgcgtgg aagcctggac cgatgtaagg gttcgaggct 2880
gtgcctttta ctgctgctgg aagggggtgg tgtgtcgccc caaaagcagg gcttcaatta 2940
agaaatgcct ctttgaaagg tgtaccttgg gtatcctgtc tgagggtaac tccagggtgc 3000
gccacaatgt ggcctccgac tgtggttgct tcatgctagt gaaaagcgtg gctgtgatta 3060
agcataacat ggtgtgtggc aactgcgagg acagggcctc tcagatgcta acctgctcgg 3120
acggcaactg tcacctgctg aagaccattc acgtagccag ccactctcgc aaggcctggc 3180
cagtgtttga gcacaacata ctgacccgct gttccttgca tttgggtaac aggagggggg 3240
tgttcctacc ttaccaatgc aatttgagtc acactaagat attgcttgag cccgaaagca 3300
tgtccaaggt gaacctgaac ggggtgtttg acatgaccat gaagatctgg aaggtgctga 3360
ggtacgatga gacccgcacc aggtgcagac cctgcgagtg tggcggtaaa catattagga 3420
accagcctgt gatgctggat gtgaccgagg agctgaggcc cgatcacttg gtgctggcct 3480
gcacccgcgc tgagtttggc tctagcgatg aagatacaga ttgaggtact gaaatgtgtg 3540
ggcgtggctt aagggtggga aagaatatat aaggtggggg tctcatgtag ttttgtatct 3600
gttttgcagc agccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat 3660
atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca 3720
ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg 3780
gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg 3840
ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat 3900
ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac 3960
ttaatgtcgt ttctcagcag ctgttggagc tgcgccagca ggtttctgcc ctgaaggctt 4020
cctcccctcc caatgcggtt taaaacataa ataaaaacca gactctgttt ggatttggat 4080
caagcaagtg tattgctgtc tttatttagg ggttttgcgc gcgcggtagg cccgggacca 4140
gcggtctcgg tcgttgaggg tcctgtgtat tttttccagg acgtggtaga gatggctctg 4200
gatgttcaga tacatgggca taagcccgtc tctggggtgg aggtagcacc actgcagagc 4260
ttcatgctgc ggggtggtgt tgtagatgat ccagtcgtag caggagcgct gggcgtggtg 4320
cctaaaaatg tcctttagaa gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt 4380
tacaaagcgg ttaagctggg atgggtgcat acgtggggat atgagatgca tcttggactg 4440
tatttttagg ttggctatgt tcccagccat atccctcctg ggattcatgt tgtgcagaac 4500
caccagcaca gtgtatccgg tgcacttggg aaatttgtca tgtagcttag aaggaaatgc 4560
gtggaagaac ttggagacgc ccttgtgacc tccaagattt tccatgcatt cgtccataat 4620
gatggcaatg ggcccacggg cggcggcctg ggcgaagata tttctgggat cactaacgtc 4680
atagttgtgt tccaggatga gatcgtcata ggccattttt acaaagcgcg ggcggagggt 4740
gccagactgc ggtataatgg ttccatccgg cccaggggcg tagttaccct cacagatttg 4800
catttcccac gctttgagtt cagatggggg gatcatgtct acctgcgggg caataaagaa 4860
aacggtttcc ggggtagggg agatcagctg ggaagaaagc aggttcctga gcagctgcga 4920
cttaccgcag ccggtgggcc cgtaaatcac acctattacc ggctgcaact ggtagttaag 4980
agagctgcag ctgccgtcat ccctgagcaa gggggccact tcgttaagca tgtccctgac 5040
acgcatgttt tccctgacca aatccgctag aaggcgctcg ccgcccagcg atagcagttc 5100
ttgcaaggaa gcaaagtttt tcaacggttt gaggccgtcc gccgtaggca tgcttttgag 5160
cgtttgacca agcagttcca ggcggtccca cagctcggtc acgtgctcta cggcatctcg 5220
atccagcata tctcctcgtt tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg 5280
tgctcgtcca gacgggccag ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta 5340
gtctgggtca cggtgaaggg gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg 5400
ctggtcctgc tggtgctgaa gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat 5460
ttgaccatgg tgtcatagtc cagcccctcc gcggcatggc ccttggcgcg cagcttgccc 5520
ttggaggagg cgccgcacga ggggcagtgc agacttttga gggcgtagag cttgggcgcg 5580
agaaataccg attccgggga gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat 5640
tccacgagcc aggtgagctc tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt 5700
ttgatgcgtt tcttacctct ggtttccatg agccggtgtc cacgctcggt gacgaaaagg 5760
ctgtccgtgt ccccgtatac agacttgaga ggcctgtcct cgagcggtgt tccgcggtcc 5820
tcctcgtata gaaactcgga ccactctgag acgaaggctc gcgtccaggc cagcacgaag 5880
gaggctaagt gggaggggta gcggtcgttg tccactaggg ggtccactcg ctccagggtg 5940
tgaagacaca tgtccccctc ttcggcatca aggaaggtga ttggtttata ggtgtatgcc 6000
acgtgaccgg gtgttcctga agggggggta taaaaggggg tgggggcgcg ttcgtcctca 6060
ctctcttccg catcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctggaaa 6120
gcgggcatga cttctgcgct aaggttgtca gtttccaaaa acgaggagga tttgatattc 6180
acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga aaagacaatc 6240
tttttgttgt caagcttggt ggcaaacgac ccgtagaggg cgttggacag caacttggcg 6300
atggagcgca gggtttggtt tttgtcgcga tcggcgcgct ccttggccgc gatgtttagc 6360
tgcacgtatt cgcgcgcaac gcaccgccat tcgggaaaga cggtggtgcg ctcgtcgggc 6420
accaggtgca cgcgccaacc gcggttgtgc agggtgacaa ggtcaacgct ggtggctacc 6480
tctccgcgta ggcgctcgtt ggtccagcag aggcggccgc ccttgcgcga gcagaatggc 6540
ggtagggggt ctagctgcgt ctcgtccggg gggtccgcgt ccacggtaaa gatcccgggc 6600
aacaggcgcg cgtcgaagta gtctatcttg catccttgca agtctaacgc ctgctgccat 6660
gcgcgggcgg caagcgcgcg ctcgtatggg ttgagtgggg gaccccatgg catggggtgg 6720
gtgagcgcgg aggcgtacat gccgcagatg tcgtaaacgt agaggggctc cctgagtatt 6780
ccaagatatg tagggtagca tcttccaccg cggatgctgg cgcgcacgta atcgtatagt 6840
tcgtgcgagg gggcgaggag ttcgggaccg aggttgctac gggcgggctg ctctgctcgg 6900
aagacgatct gcctgaagat ggcatgcgag ttcgatgata tggttggacg ctggaagacg 6960
ttgaagctgg cgtctgtaag acctaccgca tcacgcacga aggaggcgta ggagtcgcgc 7020
agcttgttga ccagctcggc ggtgacctgc acgtctaggg cacagtagtc cagggtttcc 7080
ttgatgatgt catacttatc ctgtcccttt tttttccaca gctcgcggtt gaggacaaac 7140
tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtaagag 7200
cctagcatgt agaactggtt gacggcctgg taggcgcagc atcccttttc tacgggtagc 7260
gcgtatgcct gcgcggcctt ccggagcgag gtgtgggtga gcgcaaaggt gtccctgacc 7320
atgactttga ggtactggta tttgaagtca gtgtcgtcgc atccgccctg ctcccagagc 7380
aaaaagtccg tgcgcttttt ggaacgcggg tttggtaggg cgaaggtgac atcgttgaag 7440
agtatctttc ccgcgcgagg cataaaattg cgtgtgatgc ggaagggtcc cggcacctcg 7500
gaacggttgt taattacctg ggcggcgagc acgatctcgt caaagccgtt gatgttgtgg 7560
cccacgatgt aaagttccaa gaagcgcggg gtgcccttaa tggagggcaa ttttttaagt 7620
tcttcgtagg taagctcttc aggggagttg agcccgtgct ctgacagggc ccagtctgca 7680
agatgagggt tggaagcaac gaatgagctc cacaggtcac gggccattag catttgcaga 7740
tggtcgcgaa aggtcctaaa ctggcgacct acggccattt tttctggggt gatgcagtag 7800
aaggtaagcg ggtcttgttc ccagcggtcc catccaaggt ccacggctag gtctcgcgcg 7860
gcggtcacta gaggctcatc tccgccgaat ttcatgacca gcatgaaggg cacgagctgc 7920
ttcccaaagg cccccatcca agtataggtc tctacatcgt aggtgacaaa gagacgctcg 7980
gtgcgaggat gcgagccgat cgggaagaac tggatctccc gccaccagtt ggaggagtgg 8040
ctgttgatgt ggtgaaagta gaagtccctg cgacgggccg aacactcgtg ctggcttttg 8100
taaaaacgtg cgcagtactg gcagcggtgc acgggctgta catcctgcac gaggttgacc 8160
tgacgaccgc gcacaaggaa gcagagtggg aatttgagcc cctcgcctgg cgggtttggc 8220
tggtggtctt ctacttcggc tgcttgtcct tgaccgtctg gctgctcgag gggagttacg 8280
gtggatcgga ccaccacgcc gcgcgagccc aaagtccaga tgtccgcgcg cggcggtcgg 8340
agcttgatga caacatcgcg caaatgggag ctgtccatgg tctggagctc ccgcggcgtc 8400
aggtcaggcg ggagctcctg caggtttacc tcgcatagcc gggtcagggc gcgggctagg 8460
tccaggtgat acctgatttc caggggctgg ttggtggcgg cgtcgatggc ttgcaagagg 8520
ccgcatcccc gcggcgcgac tacggtaccg cgcggcgggc ggtgggccgc gggggtgtcc 8580
ttggatgatg catctaaaag cggtgacgcg ggcgggcccc cggaggtagg gggggctcgg 8640
gacccgccgg gagagggggc aggggcacgt cggcgccgcg cgcgggcagg agctggtgct 8700
gcgcacggag gttgctggcg aacgcgacga cgcggcggtt gatctcctga atctggcgcc 8760
tctgcgtgaa gacgacgggc ccggtgagct tgaacctgaa agagagttcg acagaatcaa 8820
tttcggtgtc gttgacggcg gcctggcgca aaatctcctg cacgtctcct gagttgtctt 8880
gataggcgat ctcggccatg aactgctcga tctcttcctc ctggagatct ccgcgtccgg 8940
ctcgctccac ggtggcggcg aggtcgttgg agatgcgggc catgagctgc gagaaggcgt 9000
tgaggcctcc ctcgttccag acgcggctgt agaccacgcc cccttcggca tcgcgggcgc 9060
gcatgaccac ctgcgcgaga ttgagctcca cgtgccgggc gaagacggcg tagtttcgca 9120
ggcgctgaaa gaggtagttg agggtggtgg cggtgtgttc tgccacgaag aagtacataa 9180
cccagcgtcg caacgtggat tcgttgatat cccccaaggc ctcaaggcgc tccatggcct 9240
cgtagaagtc cacggcgaag ttgaaaaact gggagttgcg cgccgacacg gttaactcct 9300
cctccagaag acggatgagc tcggcgacag tgtcgcgcac ctcgcgctca aaggctacag 9360
gggcctcttc ttcttcaatc tcctcttcca taagggcctc cccttcttct tcttctggcg 9420
gcggtggggg aggggggaca cggcggcgac gacggcgcac cgggaggcgg tcgacaaagc 9480
gctcgatcat ctccccgcgg cgacggcgca tggtctcggt gacggcgcgg ccgttctcgc 9540
gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt atgggttggc ggggggctgc 9600
cgtgcggcag ggatacggcg ctaacgatgc atctcaacaa ttgttgtgta ggtactccgc 9660
caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt 9720
ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggcggcggt 9780
cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac 9840
ggcggatggt cgacagaagc accatgtcct tgggtccggc ctgctgaatg cgcaggcggt 9900
cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc tttgtagtag tcttgcatga 9960
gcctttctac cggcacttct tcttctcctt cctcttgtcc tgcatctctt gcatctatcg 10020
ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct tcctcccatg cgtgtgaccc 10080
cgaagcccct cattggctga agcagggcca ggtcggcgac aacgcgctcg gctaatatgg 10140
cctgctgcac ctgcgtgagg gtagactgga agtcatccat gtcaacaaag cggtggtatg 10200
cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga ccagttaacg gtctggtggc 10260
ccggctgcga gagctcggtg tatctgagac gcgagtaagc ccttgagtca aagacgtagt 10320
cgttgcaagt ccgcacgagg tactggtatc ctaccaaaaa gtgcggcggc ggctggcggt 10380
agaggggcca gcgtagggtg gccggggctc cgggggcgag gtcttccaac ataaggcgat 10440
gatatccgta gatgtacctg gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg 10500
gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa aaagtgctcc atggtcggga 10560
cgctctggcc ggtcaggcgc gcgcagtcgt tgacgctcta gaccgtgcaa aaggagagcc 10620
tgtaagcggg cactcttccg tggtctggtg gataaattcg caagggtatc atggcggacg 10680
accggggttc gagccccgta tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt 10740
gtcgaaccca ggtgtgcgac gtcagacaac gggggagcgc tccttttggc ttccttccag 10800
gcgcggcggc tgctgcgcta gctttttttg gccactggcc gcgcgcggcg taagcggtta 10860
ggctggaaag cgaaagcatt aagtggctcg ctccctgtag ccggagggtt attttccaag 10920
ggttgagtcg cgggaccccc ggttcgagtc tcgggccggc cggactgcgg cgaacggggg 10980
tttgcctccc cgtcatgcaa gaccccgctt gcaaattcct ccggaaacag ggacgagccc 11040
cttttttgct tttcccagat gcatccggtg ctgcggcaga tgcgcccccc tcctcagcag 11100
cggcaagagc aagagcagcg gcagacatgc agggcaccct ccccttctcc taccgcgtca 11160
ggaggggcaa catccgcggc tgacgcggcg gcagatggtg attacgaacc cccgcggcgc 11220
cgggcccggc actacctgga cttggaggag ggcgagggcc tggcgcggct aggagcgccc 11280
tctcctgagc gacacccaag ggtgcagctg aagcgtgaca cgcgcgaggc gtacgtgccg 11340
cggcagaacc tgtttcgtga ccgcgaggga gaggagcccg aggagatgcg ggatcgaaag 11400
ttccatgcag ggcgcgagtt gcggcatggc ctgaaccgcg agcggttgct gcgcgaggag 11460
gactttgagc ccgacgcgcg gaccgggatt agtcccgcgc gcgcacacgt ggcggccgcc 11520
gacctggtaa ccgcgtacga gcagacggtg aaccaggaga ttaactttca aaaaagcttt 11580
aacaaccacg tgcgcacgct tgtagcgcgc gaggaggtgg ctataggact gatgcatctg 11640
tgggactttg taagcgcgct ggagcaaaac ccaaatagca agccgctcat ggcgcagttg 11700
tttcttatag tgcagcacag cagggacaac gaggcattca gggatgcgct gttaaacata 11760
gtagagcccg agggccgctg gctgctcgat ttgataaata ttctgcagag catagtggtg 11820
caggagcgca gcttgagcct ggctgacaag gtggccgcca ttaactattc catgctcagt 11880
ctgggcaagt tttacgcccg caagatatac catacccctt acgttcccat agacaaggag 11940
gtaaagatcg aggggttcta catgcgcatg gcgctgaagg tgcttacctt gagcgacgac 12000
ctgggcgttt atcgcaacga gcgcatccac aaggccgtga gcgtgagccg gcggcgcgag 12060
ctcagcgacc gcgagctgat gcacagcctg caaagggccc tggctggcac gggcagcggc 12120
gatagagagg ccgagtccta ctttgacgcg ggcgctgacc tgcgctgggc cccaagccga 12180
cgcgccctgg aggcagctgg ggccggacct gggctggcgg tggcacccgc gcgcgctggc 12240
aacgtcggcg gcgtggagga atatgacgag gacgatgagt acgagccaga ggacggcgag 12300
tactaagcgg tgatatttct gatcagatga tgcaagacgc aacggacccg gcggtgcggg 12360
cggcgctgca gagccagccg tccggcctta actccacgga cgactggcgc caggtcatgg 12420
accgcatcat gtcgctgact gcgcgcaacc ctgacgcgtt ccggcagcag ccacaggcca 12480
accggctctc cgcaattctg gaagcggtgg tcccggcgcg cgcaaacccc acgcacgaga 12540
aggtgctggc gatcgtaaac gcgctggccg aaaacagggc catccggccc gatgaggccg 12600
gcctggtcta cgacgcgctg cttcagcgcg tggctcgtta caacagcggc aacgtgcaga 12660
ccaacctgga ccggctggtg ggggatgtgc gcgaggccgt ggcgcagcgt gagcgcgcgc 12720
agcagcaggg caacctgggc tccatggttg cactaaacgc cttcctgagt acacagcccg 12780
ccaacgtgcc gcggggacag gaggactaca ccaactttgt gagcgcactg cggctaatgg 12840
tgactgagac accgcaaagt gaggtgtacc agtccgggcc agactatttt ttccagacca 12900
gtagacaagg cctgcagacc gtaaacctga gccaggcttt caagaacttg caggggctgt 12960
ggggggtgcg ggctcccaca ggcgaccgcg cgaccgtgtc tagcttgctg acgcccaact 13020
cgcgcctgtt gctgctgcta atagcgccct tcacggacag tggcagcgtg tcccgggaca 13080
catacctagg tcacttgctg acactgtacc gagaggccat aggtcaggcg catgtggacg 13140
agcatacttt ccaggagatt acaagtgtta gccgcgcgct ggggcaggag gacacgggca 13200
gcctggaggc aaccctgaac tacctgctga ccaaccggcg gcagaagatc ccctcgttgc 13260
acagtttaaa cagcgaggag gagcgcattt tgcgctatgt gcagcagagc gtgagcctta 13320
acctgatgcg cgacggggta acgcccagcg tggcgctgga catgaccgcg cgcaacatgg 13380
aaccgggcat gtatgcctca aaccggccgt ttatcaatcg cctaatggac tacttgcatc 13440
gcgcggccgc cgtgaacccc gagtatttca ccaatgccat cttgaacccg cactggctac 13500
cgccccctgg tttctacacc gggggatttg aggtgcccga gggtaacgat ggattcctct 13560
gggacgacat agacgacagc gtgttttccc cgcaaccgca gaccctgcta gagttgcaac 13620
agcgcgagca ggcagaggcg gcgctgcgaa aggaaagctt ccgcaggcca agcagcttgt 13680
ccgatctagg cgctgcggcc ccgcggtcag atgctagtag cccatttcca agcttgatag 13740
ggtctcttac cagcactcgc accacccgcc cgcgcctgct aggcgaggag gagtacctaa 13800
acaactcgtt gctgcagccg cagcgcgaaa agaacctgcc tccggcgttt cccaacaacg 13860
ggatagagag cctagtggac aagatgagta gatggaagac gtatgcgcag gagcacaggg 13920
atgtgcccgg cccgcgcccg cccacccgtc gtcaaaggca cgaccgtcag cggggtctgg 13980
tgtgggagga cgatgactcg gcagacgaca gcagcgtcct ggatttggga gggagtggca 14040
acccgtttgc gcaccttcgc cccaggctgg ggagaatgtt ttaaaaaaaa aaaaagcatg 14100
atgcaaaata aaaaactcac caaggccatg gcaccgagcg ttggttttct tgtattcccc 14160
ttagtatgcg gcgcgcggcg atgtatgagg aaggtcctcc tccctcctac gagagcgtgg 14220
tgagcgcggc gccagtggcg gcggcgctgg gttccccctt cgatgctccc ctggacccgc 14280
cgttcgtgcc tccgcggtac ctgcggccta ccggggggag aaacagcatc cgttactctg 14340
agttggcacc cctattcgac accacccgtg tgtaccttgt ggacaacaag tcaacggatg 14400
tggcatccct gaactaccag aacgaccaca gcaactttct aaccacggtc attcaaaaca 14460
atgactacag cccgggggag gcaagcacac agaccatcaa tcttgacgac cggtcgcact 14520
ggggcggcga cctgaaaacc atcctgcata ccaacatgcc aaatgtgaac gagttcatgt 14580
ttaccaataa gtttaaggcg cgggtgatgg tgtcgcgctc gcttactaag gacaaacagg 14640
tggagctgaa atatgagtgg gtggagttca cgctgcccga gggcaactac tccgagacaa 14700
tgaccataga ccttatgaac aacgcgatcg tggagcacta cttgaaagtg ggcaggcaga 14760
acggggttct ggaaagcgac atcggggtaa agtttgacac ccgcaacttc agactggggt 14820
ttgacccagt cactggtctt gtcatgcctg gggtatatac aaacgaagcc ttccatccag 14880
acatcatttt gctgccagga tgcggggtgg acttcaccca cagccgcctg agcaacttgt 14940
tgggcatccg caagcggcaa cccttccagg agggctttag gatcacctac gatgacctgg 15000
agggtggtaa cattcccgca ctgttggatg tggacgccta ccaggcaagc ttgaaagatg 15060
acaccgaaca gggcgggggt ggcgcaggcg gcggcaacaa cagtggcagc ggcgcggaag 15120
agaactccaa cgcggcagcc gcggcaatgc agccggtgga ggacatgaac gatcatgcca 15180
ttcgcggcga cacctttgcc acacgggcgg aggagaagcg cgctgaggcc gaggcagcgg 15240
ccgaagctgc cgcccccgct gcggaggctg cacaacccga ggtcgagaag cctcagaaga 15300
aaccggtgat taaacccctg acagaggaca gcaagaaacg cagttacaac ctaataagta 15360
atgacagcac cttcacccag taccgcagct ggtaccttgc atacaactac ggcgaccctc 15420
aggccgggat ccgctcatgg accctgcttt gcactcctga cgtaacctgc ggctcggagc 15480
aggtatactg gtcgttgccc gacatgatgc aagaccccgt gaccttccgc tccacgagcc 15540
agatcagcaa ctttccggtg gtgggcgccg agctgttgcc cgtgcactcc aagagcttct 15600
acaacgacca ggccgtctac tcccagctca tccgccagtt tacctctctg acccacgtgt 15660
tcaatcgctt tcccgagaac cagattttgg cgcgcccgcc agcccccacc atcaccaccg 15720
tcagtgaaaa cgttcctgct ctcacagatc acgggacgct accgctgcgc aacagcatcg 15780
gaggagtcca gcgagtgacc attactgacg ccagacgccg cacctgcccc tacgtttaca 15840
aggccctggg catagtctcg ccgcgcgtcc tatcgagccg cactttttga gcaagcatgt 15900
ccatccttat atcgcccagc aataacacag gctggggcct gcgtttccca agcaagatgt 15960
ttggcggggc caagaagcgc tccgaccaac acccagtgcg cgtgcgcggg cactaccgcg 16020
cgccctgggg cgcgcacaaa cgcggccgca ctgggcgcac caccgtcgat gacgccatcg 16080
acgcggtggt ggaggaggcg cgcaactaca cgcccacgcc gccgccagtg tccaccgtgg 16140
acgcggccat tcagaccgtg gtgcgcggag cccggcgcta tgctaaaatg aagagacggc 16200
ggaggcgcgt agcacgtcgc caccgccgcc gacccggcac tgccgcccaa cgcgcggcgg 16260
cggccctgct taaccgcgca cgtcgcaccg gccgacgggc ggccatgcga gccgctcgaa 16320
ggctggccgc gggtattgtc actgtgcccc ccaggtccag gcgacgagcg gccgccgcag 16380
cagccgcggc cattagtgct atgactcagg gtcgcagggg caacgtgtac tgggtgcgcg 16440
actcggttag cggcctgcgc gtgcccgtgc gcacccgccc cccgcgcaac tagattgcaa 16500
taaaaaacta cttagactcg tactgttgta tgtatccagc ggcggcggcg cgcatcgaag 16560
ctatgtccaa gcgcaaaatc aaagaagaga tgctccaggt catcgcgccg gagatctatg 16620
gccccccgaa gaaggaagag caggattaca agccccgaaa gctaaagcgg gtcaaaaaga 16680
aaaagaaaga tgatgatgat gaacttgacg acgaggtgga actgttgcac gcgaccgcgc 16740
ccaggcggcg ggtacagtgg aaaggtcgac gcgtaagacg tgttttgcga cccggcacca 16800
ccgtagtctt tacgcccggt gagcgctcca cccgcaccta caagcgcgtg tatgatgagg 16860
tgtacggcga cgaggacctg cttgagcagg ccaacgagcg cctcggggag tttgcctacg 16920
gaaagcggca taaggacatg ctggcgttgc cgctggacga gggcaaccca acacctagcc 16980
taaagcccgt gacactgcag caggtgctgc ccgcgcttgc accgtccgaa gaaaagcgcg 17040
gcctaaagcg cgagtctggt gacttggcgc ccaccgtgca gctcatggtg cccaagcgcc 17100
aacgactgga agatgtcttg gaaaaaatga ccgtggagcc tgggctggag cccgaggtcc 17160
gcgtgcgacc aatcaagcag gtggcaccgg gactgggcgt gcagaccgtg gacgttcaga 17220
tacccaccac cagtagcact agtattgcca ctgccacaga gggcatggag acacaaacgt 17280
ccccggtcgc ctcggcggtg gcagatgccg cggtgcaggc ggccgctgcg gccgcgtcca 17340
aaacctctac ggaggtgcaa acggacccgt ggatgtttcg cgtttcagcc ccccggcgtc 17400
cgcgccgttc gaggaagtac ggcgccgcca gcgcgctact gcccgaatat gccctacatc 17460
cttccatcgc gcctaccccc ggctatcgtg gctacaccta ccgccccaga agacgagcaa 17520
ctacccgacg ccgaatcacc actggaaccc gccgccgccg tcgccgtcgc cagcccgtgc 17580
tggccccgat ttccgtgcgc agggtggctc gcgaaggagg caggaccctg gtgctgccaa 17640
cagcgcgcta ccaccccagc atcgtttaaa agccggtctt tgtggttctt gcagatatgg 17700
ccctcacctg ccgcctccgt ttcccggtgc cgggattccg aggaagaatg caccgtagga 17760
ggggcatggc cggccacggc ctgacgggcg gcatgcgtcg tgcgcaccac cggcggcggc 17820
gcgcgtcgca ccgtcgcatg cgcggcggta tcctgcccct ccttattcca ctgatcgccg 17880
cggcgattgg cgccgtgccc ggaattgcat ccgtggcctt gcaggcgcag agacactgat 17940
taaaaacaag ttgcatgtgg aaaaatcaaa ataaaaagtc tggactctca cgctcgcttg 18000
gtcctgtaac tattttgtag aatggaagac atcaactttg cgtctctggc cccgcgacac 18060
ggctcgcgcc cgttcatggg aaactggcaa gatatcggca ccagcaatat gagcggtggc 18120
gccttcagct ggggctcgct gtggagcggc attaaaaatt tcggttccac cgttaagaac 18180
tatggcagca aggcctggaa cagcagcaca ggccaaatgc tgagggacaa gttgaaagag 18240
caaaatttcc aacaaaaggt ggtagatggc ctggcctctg gcattagcgg ggtggtggac 18300
ctggccaacc aggcagtgca aaataagatt aacagtaagc ttgatccccg ccctcccgta 18360
gaggagcctc caccggccgt ggagacagtg ttgccagatg ggcgtggcga aaagcgcccg 18420
cgccccgaca gggaagagac tctggtgaca caaatcgacg agcctccctc gtacgaggag 18480
gcgctaaagc aaggcctgcc caccacccgt cccatcgcgc ccatggctac cggagtgttg 18540
ggccagcaca cacctgtaac gctggacctg cctccccccg ctgacaccca gcagaaacct 18600
gtgctgccag ggccgtccgc cgttgttgta acccgcccta gccgcgcgtc cctgcgccgt 18660
gccgccagcg gtccgcgatc gttgcggccc gtagccagtg gcaactggca aagcacactg 18720
aacagcatcg tgggtctggg ggtgcaatcc ctaaagcgcc gacgatgctt ttgaatagct 18780
aacgtgtcgt atgtgtgtca tgtatgcgtc catgtcgccg ccagaggagc tgctgagtcg 18840
ccgcgcgccc gctttccaag atggctaccc cttcgatgat gccgcagtgg tcttacatgc 18900
acatctcggg ccaggacgcc tcggagtacc tgagccccgg gctggtgcag ttcgcccgcg 18960
ccaccgagac gtacttcagc ctgaataaca agtttagaaa ccccacggtg gcgcctacgc 19020
acgacgtgac cacagaccgg tctcagcgtt tgacgctgcg gtttatcccc gtggaccgcg 19080
aggataccgc atactcgtac aaggcgcggt ttaccctggc tgtgggtgac aaccgtgtgc 19140
ttgacatggc ttccacatac tttgacattc gcggcgtgct ggaccggggc cccactttta 19200
agccctactc cggcactgcc tacaacgctc tagcccccaa aggcgctccc aattcctgcg 19260
agtgggaaca agaagaacca actcaggaaa tggctgaaga acttgaagat gaggaggagg 19320
cagaggagga ggaggcagag gaggaggcag aagcaccaca agctgatcag aaggttaaga 19380
agactcatgt atatgctcag gctcctttgg caggggaaaa aattaccgcc aatggcttac 19440
aaatagtttc tgatacccaa actgaaggca atccagtttt tgccgatccc acttatcaac 19500
ctgaacctca ggttggagaa tctcagtgga atgaagctga agcaactgca tctggaggca 19560
gagtactaaa aaagactact cccatgaaac catgctacgg atcgtatgcc agacctacaa 19620
ataaaaatgg gggtcaaggt atactagtag caaacaacca aggtgctcta gagtctaaag 19680
ttgaaatgca gttttttgcc ccctctggca ctgccatgaa tgaaagaaat gctgttcagc 19740
caagtattgt tttgtacagt gaggatgtta atatggaaac tcctgatact cacatttcat 19800
acaaaccaag caaaactgat gaaaactcta aggctatgtt gggtcaacaa gcaatgccaa 19860
acagacccaa ttacatcgct tttagggaca attttattgg ccttatgtat tacaacagca 19920
ctggtaacat gggtgtcctt gctggacaag catcacagct aaatgccgtg gtagacttgc 19980
aggacagaaa cacagagctg tcatatcaac ttttgcttga ttctattggc gatagaacca 20040
gatacttttc catgtggaat caggctgtag acagctatga tccagatgtc agaatcattg 20100
aaaatcatgg aactgaggat gagttgccaa attactgctt cccacttggc ggtatagggg 20160
taactgacac ctaccaaggt ataaaatcaa acggaaacgg taatcctcaa aactggacca 20220
aaaatgacga ttttgcggca cgtaatgaaa taggtgtggg aaacaacttt gccctggaga 20280
ttaaccttaa tgccaaccta tggagaaatt tcctctactc caacattgca ctgtacctgc 20340
ctgacaagct aaaatacact cctacaaatg tggaaatatc tcccaaccct aattcatacg 20400
attatatgaa caagcgagtg gtggctcccg ggttggtgga ttgctacatt aaccttggag 20460
cgcgttggtc attggactac atggacaacg tcaacccctt taaccatcac cgcaatgcgg 20520
gcctacgcta ccgctccatg ttgctgggca acggtcgcta cgtgcccttt cacatccagg 20580
ttcctcagaa gttttttgcc attaagaacc tcctactctt gccgggctca tacacctacg 20640
agtggaactt caggaaagat gttaacatgg tcctgcaaag ctccctagga aacgacctaa 20700
gagttgacgg agccagcatt aagtttgaca gcatttgcct ctacgccacc ttttttccga 20760
tggcccacaa caccgcctca acgcttgaag ccatgcttag aaacgacacc aacgaccagt 20820
cctttaacga ctacctatcc gccgccaaca tgctttaccc catacccgcc aacgccacca 20880
acgtgcccat ctctatcccc tcgcgcaact gggcggcttt ccgaggctgg gcctttacgc 20940
gccttaagac taaggaaacc ccatccctgg gttccggcta cgacccttac tatacctact 21000
ctggctccat accctaccta gacggaacct tttaccttaa tcacaccttc aaaaaggtag 21060
ccatcacctt tgactcttct gttagctggc ctggcaatga ccgtctgctt acccccaacg 21120
agtttgagat caagcgttca gttgacggag agggctacaa cgttgcccaa tgcaacatga 21180
ccaaagactg gttcttggta cagatgctag ccaactacaa cataggctac cagggctttt 21240
atatcccaga aagctataag gaccgcatgt actccttctt tagaaacttc cagcccatga 21300
gccgtcaggt ggtggacgat accaaataca aggactacca acaggtgggc atcctccacc 21360
agcacaataa ctctggcttt gttggttacc tcgctcccac catgcgagag ggacaggcct 21420
accccgccaa cttcccctac ccgcttatag gcaagaccgc ggttgacagt attacccaga 21480
aaaagtttct ttgcgaccgc accctttggc gcattccatt ctccagtaac tttatgtcca 21540
tgggtgcact cacagacctg ggccaaaacc ttctctatgc aaactccgcc cacgcgctag 21600
atatgacttt tgaggtggat cccatggacg agcccaccct tctttatgtt ttgtttgaag 21660
tctttgacgt ggtccgtgtg caccagccgc accgcggcgt catcgagacc gtgtacctgc 21720
gcacgccctt ctcggctggc aacgccacaa cataaagaag caagcaacat caacaacagc 21780
tgccgccatg ggctccagtg agcaggaact aaaagccatt gtcaaagatc ttggttgtgg 21840
accatatttt ttgggcacct atgacaagcg cttcccaggc tttgtttccc cacacaagct 21900
cgcctgcgcc atagttaaca cggccggtcg cgagactggg ggtgtacact ggatggcctt 21960
tgcctggaac ccgcgctcaa aaacatgcta cctctttgag ccctttggct tttctgacca 22020
acgtctcaag caggtttacc agtttgagta cgagtcactc ctgcgccgta gcgccattgc 22080
ctcttccccc gaccgctgta taacgctgga aaagtccacc caaagcgtgc aggggcccaa 22140
ctcggccgcc tgtggcctat tctgctgcat gtttctccac gcctttgcca actggcccca 22200
aactcccatg gatcacaacc ccaccatgaa ccttattaca ggggtaccca actccatgct 22260
taacagtccc caggtacagc ccaccctgcg ccgcaaccag gaacagctct acagcttcct 22320
ggagcgccac tcgccctact tccgcagcca cagtgcgcaa attaggagcg ccacttcttt 22380
ttgtcacttg aaaaacatgt aaaaataatg tactaggaga cactttcaat aaaggcaaat 22440
gtttttattt gtacactctt gggtgattat ttacccccca cccttgccgt ctgcgccgtt 22500
taaaaatcaa aggggttctg ccgcgcatcg ctatgcgcca ctggcaggga cacgttgcga 22560
tactggtgtt tagtgctcca cttaaactca ggcacaacca tccgcggcag ctcggtgaag 22620
ttttcactcc acaggctgcg caccatcacc aacgcgttta gcaggtcggg ccccgatatc 22680
ttgaagtcgc agttggggcc tccgccctgc gcgcgcgagt tgcgatacac agggttgcag 22740
cactggaaca ctatcagcgc cgggtgatgc acgctggcca gcacgctctt gtcggagatc 22800
agatccgcgt ccaggtcctc cgcgttgctc agggcgaacg gagtcaactt tggtagctgc 22860
cttcccaaaa agggtgcatg cccaggcttt gagttgcact cgcaccgtag tggcatcaga 22920
aggtgaccgt gcccggtctg ggcgttagga tacagcgcct tcataaaggc ctggatctgc 22980
ttaaaagcca cctgggcctt tgcgccttcg gaaaaaaaca tgccgcaaga cttgccggaa 23040
aactgattcg ccggacaggc cgcgtcgtgc acgcagcacc tcgcgtcggt gttggagatc 23100
tgtaccacgc ttcggcccca acggttcttc acaatcttgg ccttgctaga ctgctccttc 23160
agcgcgcgct gcccgttttc gctcgtcaca tccatttcaa tcacgtgctc cttgtttatc 23220
ataatgctcc cgtgtagaca cttaagctcg ccttcgatct cagcgcagcg gtgcagccac 23280
aacgcgcagc ccgtgggctc gtggtgcttg taggtcacct ctgcaaacga ctgcaggtac 23340
gcttgcagga atcgccccat catcgtcaca aaggtcttgt tgctggtaaa ggtcagctgc 23400
aacccgcggt gctcctcgtt cagccaggtc ttacatacgg ccgccagggc ttccacttgg 23460
tcaggcagta gcttgaagtt cgcctttaga tcgttatcca cgtgatactt gtccatcagc 23520
gcgcgcgcag cctccatgcc cttctcccac gcagacacga tcggcagact cagcgggttc 23580
attaccgtgc tttcactttc cgcttcgctg ggctcttcct cttcctcttg catccgcata 23640
ccccgcgcca ctgggtcgtc ttcattcagc cgccgcaccg tgcgcttacc tcccttgccg 23700
tgcttaatta gcaccggtgg gttgctgaaa cccaccattt gtagcgccac atcttctctt 23760
tcttcctcgc tgtccacaat cacctctggg gatggcgggc gctcgggctt gggagatggg 23820
cgcttctttt tcttcttggg cgcgttggcc aaatccgccg ccgaggttga tggccgcggg 23880
ctgggtgtgc gcggcaccag cgcgtcttgt gacgagtctt cttcgtcctc ggactcgaga 23940
cgccgcctca gccgcttttt tgggggcgcg cggggaggcg gcggcgacgg cgacggggac 24000
gacacgtcct ccatggttgg tggacgtcgc gccgcaccgc gtccgcgctc gggggtggtt 24060
tcgcgctgct cctcttcccg actggccatt tccttctcct ataggcagaa aaagatcatg 24120
gagtcagtcg agaaggagga cagcctaacc gccccctctg agttcgccac caccgcctcc 24180
accgatgccg ccaacgcgcc taccaccttc cccgtcgagg cacccccgct tgaggaggag 24240
gaagtgacta tcgagcagga cccaggtttt gtaagcgaag acgacgagga ccgctcagta 24300
ccaacagagg ataaaaagca agaccaggac gacgcagagg caaacaagga gcaagtcggg 24360
cggggggacg agaggcatgg cgactaccta gatgtgggag acgacgtgct gttgaagcat 24420
ctgcagcgcc agtgcgctat tatctgcgac gcgttgcaag agcgcagcga tgtgcccctc 24480
gccatagcgg atgttagcct tgcctacgaa cgccacctgt tctcaccgcg tgtacccccc 24540
aaacgacaag aaaacggcac atgcgagccc aacccgcgcc tcaacttcta ccccgtattt 24600
gccgtgccag aggtgcttgc cacctatcac atctttttcc aaaactgcaa gataccccta 24660
tcctgccgtg ccaaccgcag ccgagcggac aagcagctgg ccttgcggca gggcgctgtc 24720
atacctgata tcgcctcgct cgacgaagtg ccaaaaatct ttgagggtct tggacgcgac 24780
gagaagcgcg cggcaaacgc tctgcaacaa gaaaacagcg aaaatgaaag tcattgtgga 24840
gtgctggtgg aacttgaggg tgacaacgcg cgcctagccg tgctaaaacg cagcatcgag 24900
gtcacccact ttgcctaccc ggcacttaac ctacccccca aggttatgag cacagtcatg 24960
agcgagctga ttgtgcgccg tgcacaaccc ctggagaggg atgcaaactt gcaagaacaa 25020
accgaggagg gcctacccgc agttggcgat gagcagctgg cgcgctggct tgagacgcgc 25080
gagcctgccg acttggagga gcgacgcaag ctaatgatgg ccgcagtgct tgttaccgtg 25140
gagcttgagt gcatgcagcg gttctttact gacccgaaga tgcagcgcaa gctagaggaa 25200
acattgcact acacctttcg ccagggctac gtgcgccagg cctgcaaaat ttccaacgtg 25260
gagctctgca acctggtctc ctaccttgga attttgcacg aaaaccgcct cgggcaaaac 25320
gtgcttcatt ccacgctcaa gggcgaggcg cgccgcgact acgtccgcga ctgcgtttac 25380
ttatttctat gctacacctg gcaaacggcc atgggcgtgt ggcagcagtg cctggaggag 25440
cgcaacctga aggagctgca gaagctgcta aagcaaaact tgaaggacct atggacggcc 25500
ttcaacgagc gctccgtggc cgcgcacctg gcggacatta tcttccccga acgcctgctt 25560
aaaaccctgc aacagggtct gccagacttc accagtcaaa gcatgttgca aaactttagg 25620
aactttatcc tagagcgttc aggaatcctg cccgccacct gctgtgcgct tcctagcgac 25680
tttgtgccca ttaagtaccg tgaatgccct ccgccgcttt ggggtcactg ctaccttctg 25740
cagctagcca actaccttgc ctaccactcc gacatcatgg aagacgtgag cggtgacggc 25800
ctactggagt gtcactgtcg ctgcaaccta tgcaccccgc accgctccct ggtctgcaat 25860
tcgcagctgc ttagcgagag tcaaattatc ggtacctttg agctgcaggg tccctcgcct 25920
gacgaaaagt ccgcggctcc ggggttgaaa ctcactccgg ggctgtggac gtcggcttac 25980
cttcgcaaat ttgtacctga ggactaccac gcccacgaga ttaggttcta cgaagaccaa 26040
tcccgcccgc ccaatgcgga gcttaccgcc tgcgtcatta cccagggcca catccttggc 26100
caattgcaag ccatcaacaa agcccgccaa gagtttctgc tacgaaaggg acggggggtt 26160
tacttggacc cccagtccgg cgaggagctc aacccaattc ccccgccgcc gcagccctat 26220
cagcagcagc cgcgggccct tgcttcccag gatggcaccc aaaaagaagc tgcagctgcc 26280
gccgccaccc acggacgagg aggaatactg ggacagtcag gcagaggagg ttttggacga 26340
ggaggaggag gacatgatgg aagactggga gagcctagac gaggaagctt ccgaggccga 26400
agaggtgtca gacgaaacac cgtcaccctc ggtcgcattc ccctcgccgg cgccccagaa 26460
atcggcaacc ggtccaagca tggctacaac ctccgctcct caggcgccgc cggcactgcc 26520
cgttcgccga cccaaccgta gatgggacac cactggaacc agggccggta agtccaagca 26580
gccgccgccg ttagcccaag agcaacaaca gcgccaaggc taccgctcgt ggcgcgggca 26640
caagaacgcc atagttgctt gcttgcaaga ctgtgggggc aacatctcct tcgcccgccg 26700
ctttcttctc taccatcacg gcgtggcctt cccccgtaac atcctgcatt actaccgtca 26760
tctctacagc ccctactgca ccggcggcag cggcagcaac agcagcggcc acacagaagc 26820
aaaggcgacc ggatagcaag actctgacaa agcccaagaa atccacagcg gcggcagcag 26880
caggaggagg agcgctgcgt ctggcgccca acgaacccgt atcgacccgc gagcttagaa 26940
acaggatttt tcccactctg tatgctatat ttcaacagag caggggccaa gaacaagagc 27000
taaaaataaa aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat cacaaaagcg 27060
aagatcagct tcggcgcacg ctggaagacg cggaggctct cttcagtaaa tactgcgcgc 27120
tgactcttaa ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa ctacgtcatc 27180
tccagcggcc acacccggcg ccagcacctg tcgtcagcgc cactatgagc aaggaaattc 27240
ccacgcccta catgtggagt taccagccac aaatgggact tgcggctgga gctgcccaag 27300
actactcaac ccgaataaac tacatgagcg cgggacccca catgatatcc cgggtcaacg 27360
gaatacgcgc ccaccgaaac cgaattctcc tggaacaggc ggctattacc accacacctc 27420
gtaataacct taatccccgt agttggcccg ctgccctggt gtaccaggaa agtcccgctc 27480
ccaccactgt ggtacttccc agagacgccc aggccgaagt tcagatgact aactcagggg 27540
cgcagcttgc gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt ataactcacc 27600
tgaaaatcag agggcgaggt attcagctca acgacgagtc ggtgagctcc tcgcttggtc 27660
tccgtccgga cgggacattt cagatcggcg gtgccggccg ctcttcattt acgccccgtc 27720
aggcgatctt aactctgcaa acctcatcct cggagccgcg ctccggaggc attggaactc 27780
tacaatttat tgaggagttc gtgccttcgg tttacttcaa ccccttttct ggacctcctg 27840
gccactaccc ggaccagttt attcccaact ttgacgcggt gaaggactcg gcggacggct 27900
acgactgaat gactagtgga gaggcagagc aactgcgcct aacacacctt gaccactgcc 27960
gccgccacaa gtgctttgcc cgcggctccg gtgagttttg ttactttgag ttgcccgaag 28020
agcatatcga gggcccggcg cacggcgtcc ggctcaccac ccaggtagag cttactcgta 28080
gcctaattcg ggagtttacc ctgcgccccc tgctagtgga gcgggagagg ggtccctgtg 28140
ttctgaccgt ggtttgcaac tgtcctaacc ctggattaca tcaagatctt tgttgccatc 28200
tctgtactga gtataataaa tacagaaatt agaatctact ggggctcctg tcgctcctgt 28260
aaacgccacc gtcttcaccc acccaaacct gcgatttgca caggcgggcc aaaaagtacc 28320
ttgccggtac ttaacggctc ttcctttgtg atttacaaca gtttcaacca agacggagtg 28380
agccgcggac aacctctccg ggcttatcag aaaaaatcac cctccttacc tgccgggaac 28440
gtaccagtgc gtcaccggtc gccgcaccca ccgcctgatt gttaaccaac atttccagca 28500
acagacctca ataattccca ttttccataa gaggaggtga gcttaacccc ctggaagcgc 28560
atttttactg tttttaggca aggtgtatga actctactaa ctctacgggc ttctctgctt 28620
caggtaattt ttctggaatc gggctcgggg tcattctcac tcttgtaatt ctctttattt 28680
ttatactagc gcttctgtgt cttaggcttg ccgcctgctg cgcacacgtt tgcatttact 28740
gtcagcttat taaacgctgg ggacgccacc caagatgatt aaatacatca ttttaagctt 28800
gcttacccta gcggcagtct gcagtgctgc caaacaggta gagtttaagg agccagcttg 28860
caatgttaca tttaaatccg aagctaatga atgcaccact cttataaaat gcaccacaga 28920
acatgaaaag cttattattc gccacaaaga caaagctggc aagtatgctg tatatgctat 28980
ttggcagcct ggagacccta acaactataa tgttacagtt tttcagggta atgattctaa 29040
agtttttttg tatgaatttc cattttatga aatgtgtgac attaccatgt acatgagcaa 29100
acagtataag ttgtggcccc cacaaaagtg tgtggagaac actggcactt tttgctttac 29160
tgctctcctg attacagtga ttactttggt gtgtaccctc ctttatatta aatttaaaag 29220
cagacgcagc tttattgaag aaaagaaaat gccttagcat gctgccgccg cttcttgctt 29280
gcattgcaaa acagcttaca accttagcat tataatagca tgtcttgcaa cgcccccctc 29340
aaagctcccc actttaccct gcttaatgtc attcccatgg acaatggact ctgtggaata 29400
tgtgccaggt tggcaaggca agacacacgc ctacaacctt aaagtcaggc tttcctggac 29460
tgagagcgcc tgacttcaac agcacctgcc ccgtggagtt catccagcaa cttcagcagc 29520
ccgccttcga catggtcgac acagtgaact cttacaacac cgctaccgga ctcacatcta 29580
cccaagatat gccccaagtt tctacctttg ttaataattg ggcaaacttg ggcatgtggt 29640
ggttttcaat agcgcttatg tttgtctgcc ttattattat gtggctttcc tgctgcctaa 29700
agcgcaaacg cgcccgacca cccatttata agcccattat tgtgcttaac ccaaacaatg 29760
atggaataca tagattggac gggctgaaca cttgttcttt ttcttttgca gtatgattaa 29820
atgagacatg attcctcgag tttttatatt attgaccctt attgcgcttt tctgcgcgtg 29880
ctccacattg gccgcggttg ctaacatcga agtagactgc attccagcct tcacagtcta 29940
cctgctttac ggatttgtca cccttacgct catctgcagc ctcatcactg tagtgatcgc 30000
ctttattcaa tgcattgact gggtctgtgt gcgcattgca taccttagac accatcctca 30060
atacagagac acggccatag ctgagcttct tagaattctt taattatgaa aattacttgg 30120
attttcttac taatttttta caccttaact gtggtttgct cccagacctc caagcctccc 30180
caaagacatg tatcttgcag atttactcgt atatggaaca ttccaagttg ctacactgaa 30240
aaaagcgacc tgtccgaaac ctggttgtat gccatcatct cggtttttgt attttgtagc 30300
accataattg cactagccat atacccctac ctggatattg gatggaacgc catagacgcc 30360
atgagccatt ccactttccc cgcgcccgct ataattcaga tgcagccaat tgttgccggc 30420
ggcattgtcc cagccaatca gcatcgccct ccttctccca ctcccactga gattagctac 30480
tttaatctaa caggcggaga tgactgacgg cctagatcta gaaatggacg gaattattac 30540
cgagcagcgt ctgctacaga ggcgcaaagc agcggcagag caggaacgcc taaatcagga 30600
gctccaagac atggttaacc tgcaccagtg taaaagaggt attttttgtc tggttaagca 30660
ggcaaaagtt acctacgaga aggaccacag caaccaccgc cttagctaca agctgcccgc 30720
caagcgccaa aaactgctgc ttatggtggg agaaaggccc atcaccgtca ctcagcagtc 30780
agtagaaact gagggctgtc tgcacttccc ctgccagggt ccagaggacc tttgcaccct 30840
tattaagact ttatgcggtc ttagagatct tattcccttt aactaataaa caatacaata 30900
aatgtaatca ataaagaatc acttacttaa aatcacttag cagatcttta tccaatttat 30960
tcagcagcac ctcctttccc tcctcccaac tctggtacct cagtcgtctt ctagctgcaa 31020
actttctcca cagtttaaat gggatgtcag attcctcttg ttcctgcccc tccgcaccca 31080
ctattttcat gttgttgcag atgaagcgcg ccagaccgtc tgaagacacc ttcaaccccg 31140
tgtaccctta tgacacggaa accggccccc caacagtgcc tttccttacc cctccctttg 31200
tctcccccaa tgggttccaa gaaagtcccc ctggggtgct ttctctgcgc ctttccgaac 31260
cactcgtcac ctcccacggc atgcttgcgc tcaaaatggg cagcggccta gccctggaca 31320
aggccggcaa ccttacatca caaaatataa ccaccgtcac tgagccatta aaaaaaacaa 31380
agtccaacat aagcctggaa acctccgcgc ccctcacagt tacatcaggc attcttaccg 31440
tagccactgc cgcacctttg gtggtcgcgg gcaacagtct taccgtgcag tcacaggccc 31500
cgttaaccgt gcaagactct aaacttagca ttgccaccaa aggacccctc acagtgtcag 31560
aaggaaagct ggccctgcaa acatcgggcc ccctttctgc cactgataac aacaccctta 31620
ctattactac ttcaccccct ataactacta caaatggtag tttgggtgtt aacatggaaa 31680
acccacttta caacaacaat ggcaaactgg ggctcagagt tgcaggtcct ttgcaagtga 31740
ccaacgactc tcacgcacta acagtaggta caggtcaggg tgttgccatt gataataatg 31800
cgctacacac aaaagttaca ggcgcaatag ggtatgatac atctggtaac atggaactta 31860
aaactggagg gggagtgcgt gtagacagtg taaataggcg tcttatactc gatgttgatt 31920
atccatttga tgcccaaagc caactacgcc taaaactagg acagggaccc ctatatgtaa 31980
actcaagcac acacaacttg gatttaaact acaataaagg ccttcacttg tttacaactg 32040
gaaactctaa aaagctagaa gtaaatctta aaacaacaaa gggtcttatt tttgacaccg 32100
atgctgttgc aatacatgca gcacaaggtc tagaatttgg taatgatacg tcaacaaata 32160
caaacccact taaaacaaaa ctaggattgg gattggacta tgactcaaac ggtggtatga 32220
ttcccaaact tggaacaggc cttagttttg atactacagg ggctataaca gtgggaaaca 32280
aaagcgatga caaacttacc ctgtggacaa ctcctgaccc atcacctaat tgtcaaatac 32340
actcagaaaa agatgctaag ctaacactag ttttaactaa atgcggcagt caggtacttg 32400
caactgtgtc agcactggct gtaaggggca gcctagctcc tattagcgga acaataagta 32460
gtgctcacat tattctcaga tttaatgaac atggagtgct aatgaatcat tctagtttgg 32520
atccccaata ctggaatttc agaaaagggg atttaacaaa cgctacagca tatactaacg 32580
cagttggttt tatgcccaac cttaaagctt acccaaaaac tcaaagtaga actgcaaaaa 32640
gcaacattgt tagtcaggtt tatcttaatg gagaaaaaga aaaaccaatg acactcacta 32700
ttacacttaa tggaactgat gaaaatcaaa ccactcccgc cagtacatac tcaatttcat 32760
tttcatggag ctggcctagc aatcaaacat acattggtca aacatttgcc actaattcct 32820
acaccttctc ctacattgcc caagaataaa gaatcgttgt gttatgtttc aacgtgttta 32880
tttttcaatt gcaaaaaatt tcaagtcatt tttcattcag tagtatagcc ccaccaccac 32940
atagcttata ttgatcaccg taccttaatc aaactcacag aaccctagta ttcaacctgc 33000
cacctccctc ccaacacaca gagtacacag tcctttctcc ccggctggcc ttaaaaagca 33060
tcatatcatg ggtaacagac atattcttag gtgttatatt ccacacggtt tcctgtcgag 33120
ccaaacgctc atcagtgata ttaataaact ccccgggcag ctctcttaag ttcatgttgc 33180
tgtccagctg ctgagccaca ggctgctgtc caacttgcgg ttgctcaacg ggcggcgaag 33240
gggaagtcca cgcctacatg ggggtagagt cataatcgtg catcaggata gggcggtggt 33300
gctgcagcag cgcgcgaata aactgctgcc gccgccgctc cgtcctgcag gaatacaaca 33360
tggcagtggt ctcctcagcg atgattcgca ccgcccgcag cataagacgc cttgtcctcc 33420
gggcacagca gcgcaccctg atctcactta aatcagcaca gtaactgcag cacagcacca 33480
caatattgtt caaaatccca cagtgcaagg cgctgtatcc aaagctcatg gcggggacca 33540
cagaacccac gtggccatca taccacaagc gcaggtagat taagtggcga cccctcataa 33600
acacgctgga cataaacatt acctcttttg gcatgttgta attcaccacc tcccggtacc 33660
atataaacct ctgattaaac atggcgccat ccaccaccat cctaaaccag ctggccaaaa 33720
cctgcccgcc ggctatgcac tgcaaggaac cgggactgga acaatgacag tggagagccc 33780
aggactcgta accatggatc atcatgctcg tcatgatatc aatgttggca caacacaggc 33840
acacgtgcat acacttcctc aggattacaa gctcctcccg cgtcagaacc atatcccagg 33900
gaacaaccca ttcctgaatc agcgtaaatc ccacactgca gggaagacct cgcacgtaac 33960
tcacgttgtg cattgtcaaa gtgttacatt cgggcagcag cggatgatcc tccagtatgg 34020
tagcgcgggt ctctgtctca aaaggaggta ggcgatccct actgtacgga gtgcgccgag 34080
acaaccgaga tcgtgttggt cgtagtgtca tgccaaatgg aacgccggac gtagtcatat 34140
ttcctgaagc aaaaccaggt gcgggcgtga caaacagatc tgcgtctccg gtctcgtcgc 34200
ttagctcgct ctgtgtagta gttgtagtat atccactctc tcaaagcatc caggcgcccc 34260
ctggcttcgg gttctatgta aactccttca tgcgccgctg ccctgataac atccaccacc 34320
gcagaataag ccacacccag ccaacctaca cattcgttct gcgagtcaca cacgggagga 34380
gcgggaagag ctggaagaac catgtttttt ttttttttat tccaaaagat tatccaaaac 34440
ctcaaaatga agatctatta agtgaacgcg ctcccctccg gtggcgtggt caaactctac 34500
agccaaagaa cagataatgg catttgtaag atgttgcaca atggcttcca aaaggcaaac 34560
tgccctcacg tccaagtgga cgtaaaggct aaacccttca gggtgaatct cctctataaa 34620
cattccagca ccttcaacca tgcccaaata attttcatct cgccacctta tcaatatgtc 34680
tctaagtaaa tcccgaatat taagtccggc cattgtaaaa atctgctcca gagcaccctc 34740
caccttcagc ctcaagcagc gaatcatgat tgcaaaaatt caggttcctc acagacctgt 34800
ataagattca aaagcggaac attaacaaaa ataccgcgat cccgtaggtc ccttcgcagg 34860
gccagctgaa cataatcgtg caggtctgca cggaccagcg cggccacttc cccgccagga 34920
accatgacaa aagaacccac actgattatg acacgcatac tcggagctat gctaaccagc 34980
gtagccccga tgtaagcttg ttgcatgggc ggcgatataa aatgcaaggt gctgctcaaa 35040
aaatcaggca aagcctcgcg caaaaaagca agcacatcgt agtcatgctc atgcagataa 35100
aggcaggtaa gttccggaac caccacagaa aaagacacca tttttctctc aaacatgtct 35160
gcgggttcct gcataaacac aaaataaaat aacaaaaaaa aaaacattta aacattagaa 35220
gcctgtctta caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg 35280
gcgtgaccgt aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ttcctcggtc 35340
atgtccggag tcataatgta agactcggta aacacatcag gttggttaac atcggtcagt 35400
gctaaaaagc gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt 35460
acagccccca taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa 35520
aaaccctcct gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc 35580
acagcggcag ccataacagt cagccttacc agtaaaaaaa cctattaaaa aacaccactc 35640
gacacggcac cagctcaatc agtcacagtg taaaaagggc caagtacaga gcgagtatat 35700
ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg 35760
cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cttcacttcc 35820
gttttcccac gatacgtcac ttcccatttt aaaaaaaact acaattccca atacatgcaa 35880
gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca 35940
aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat 36000
g 36001
<210> 12
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> INFA-F85 primer
<220>
<221> misc_feature
<222> (21)..(25)
<223> n denotes deoxyinosine
<400> 12
aggccccctc aaagccgaga nnnnncagag acttg 35
<210> 13
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> INFA-R598 primer
<220>
<221> misc_feature
<222> (24)..(28)
<223> n denotes deoxyinosine
<400> 13
atagccttag ctgtagtgct ggcnnnnncc attctgtt 38
<210> 14
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> INFB-5'-955
<220>
<221> misc_feature
<222> (12)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (18)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (25)..(29)
<223> n denotes deoxyinosine
<400> 14
ttggctatga cngaaagnat aaccnnnnnc agcccaa 37
<210> 15
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> INFB-3'-1409
<220>
<221> misc_feature
<222> (18)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (26)..(30)
<223> n denotes deoxyinosine
<400> 15
ttacatgttc ggtaaaantc gtttannnnn tccatacatg 40
<210> 16
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> PIV1-HN-F1
<220>
<221> misc_feature
<222> (24)..(28)
<223> n denotes deoxyinosine
<400> 16
gacccacatg atttctggag atgnnnnnta ggagaacc 38
<210> 17
<211> 43
<212> DNA
<213> Artificial Sequence
<220>
<223> PIV1-HN-R1
<220>
<221> misc_feature
<222> (29)..(33)
<223> n denotes deoxyinosine
<400> 17
ctattacaga acatgatttc ctgttgtcnn nnntgtcata ggt 43
<210> 18
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> hPIV-2-5'
<220>
<221> misc_feature
<222> (19)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (26)..(30)
<223> n denotes deoxyinosine
<400> 18
tggacctctc ctaaatatnc ccagcnnnnn cccctcagca 40
<210> 19
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> hPIV-2-3'
<220>
<221> misc_feature
<222> (25)..(29)
<223> n denotes deoxyinosine
<400> 19
gtgactgaac agcttttgcg attgnnnnna tcacttagg 39
<210> 20
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> hPIV-3-5'
<220>
<221> misc_feature
<222> (5)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (26)..(30)
<223> n denotes deoxyinosine
<400> 20
taagnataaa atggacatgg cataannnnn tatcaagacc 40
<210> 21
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> hPIV-3-3'
<220>
<221> misc_feature
<222> (8)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (14)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (27)..(31)
<223> n denotes deoxyinosine
<400> 21
cgtttacnct ttcngttgct gttgagnnnn ntatgactgg g 41
<210> 22
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> RSVa-F103
<220>
<221> misc_feature
<222> (27)..(31)
<223> n denotes deoxyinosine
<400> 22
agaattttat caatcaacat gcagtgnnnn nagcaaaggc t 41
<210> 23
<211> 45
<212> DNA
<213> Artificial Sequence
<220>
<223> RSVa-R382
<220>
<221> misc_feature
<222> (31)..(35)
<223> n denotes deoxyinosine
<400> 23
attgttgagt gtataattca taaaccttgg nnnnnctctt ctggc 45
<210> 24
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> RSVb-F536
<220>
<221> misc_feature
<222> (25)..(29)
<223> n denotes deoxyinosine
<400> 24
tcagtctatc aaatggggtc agtgnnnnna ccagcaaag 39
<210> 25
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> RSVb-R927
<220>
<221> misc_feature
<222> (30)..(34)
<223> n denotes deoxyinosine
<400> 25
attacaccat agataggtag ctgtacaacn nnnncaagga cttc 44
<210> 26
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> hMPV-5'-585
<220>
<221> misc_feature
<222> (23)..(27)
<223> n denotes deoxyinosine
<400> 26
agcttcagtc aattcaacag aannnnncta aatgttg 37
<210> 27
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> hMPV-3'-1007
<220>
<221> misc_feature
<222> (5)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (14)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (23)..(27)
<223> n denotes deoxyinosine
<400> 27
ttgantgctc agcnacatta atnnnnncwg ctgtgtc 37
<210> 28
<211> 40
<212> DNA
<213> Artificial Sequence
<220>
<223> hCoVOC43-F400
<220>
<221> misc_feature
<222> (26)..(30)
<223> n denotes deoxyinosine
<400> 28
tatgttaggc cgataattga ggactnnnnn actctgacgg 40
<210> 29
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> hCoVOC43-3'
<220>
<221> misc_feature
<222> (27)..(31)
<223> n denotes deoxyinosine
<400> 29
gtaattaccg actttggact taacatnnnn ngcaaaacca c 41
<210> 30
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> 229E-F375
<220>
<221> misc_feature
<222> (2)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (27)..(31)
<223> n denotes deoxyinosine
<400> 30
tncttaagca gtatacttct gcttgtnnnn nctattgaag a 41
<210> 31
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> 229E-R375
<220>
<221> misc_feature
<222> (8)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (25)..(29)
<223> n denotes deoxyinosine
<400> 31
tggccatncg ttcagcatca gcgannnnng gcaaaacca 39
<210> 32
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> HRV-F151
<220>
<221> misc_feature
<222> (23)..(27)
<223> n denotes deoxyinosine
<400> 32
ccccacgggt gaccgtgtcc cannnnncgt ggcggcc 37
<210> 33
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> HRV-R151
<220>
<221> misc_feature
<222> (24)..(28)
<223> n denotes deoxyinosine
<400> 33
gctccagggt taaggttagc cgcnnnnngg ggccgg 36
<210> 34
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> HAdV-F1
<220>
<221> misc_feature
<222> (5)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (23)..(27)
<223> n denotes deoxyinosine
<400> 34
tctanggcat ctcgatccag cannnnncct cgtttcg 37
<210> 35
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> HAdV-R1
<220>
<221> misc_feature
<222> (7)..(8)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (18)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (26)..(30)
<223> n denotes deoxyinosine
<400> 35
ggacacnngc tcatgganac caragnnnnn aaacgcatca a 41
<210> 36
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> HRVa-F15
<220>
<221> misc_feature
<222> (21)..(25)
<223> n denotes deoxyinosine
<400> 36
gaaacacgga cacccaaagt nnnnngtccc rtccc 35
<210> 37
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> HRV-R353
<220>
<221> misc_feature
<222> (8)
<223> n denotes deoxyinosine
<220>
<221> misc_feature
<222> (22)..(26)
<223> n denotes deoxyinosine
<400> 37
atcgttancc gcaaagtgcc tnnnnnaagc ytagta 36
Claims (19)
- 다음의 일반식으로 표시되는, 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드:5'-Xp-Yq-Zr-3'상기 일반식에서, Xp는 혼성화되는 타깃 서열과 실질적으로 상보적인 혼성화 서열을 가지는 5'-고 Tm 특이성 구역 (5'-high Tm specificity portion)이고, Yq는 최소 두 개 이상의 유니버설 염기를 포함하는 분할 구역 (separation portion)이며, Zr은 혼성화되는 타깃 서열과 실질적으로 상보적인 혼성화 서열을 가지는 3'-저 Tm 특이성 구역 (3'-low Tm specificity portion)이고, p, q 및 r은 뉴클레오타이드의 개수를 나타내며, X, Y 및 Z는 디옥시리보뉴클레오타이드 또는 리보뉴클레오타이드이고, 5'-고 Tm 특이성 구역의 Tm은 3'-저 Tm 특이성 구역의 Tm보다 높으며, 상기 분할 구역은 상기 세 구역 중에서 가장 낮은 Tm을 가지고, 5'-고 Tm 특이성 구역과 3'-저 Tm 특이성 구역이 타깃 서열에 혼성화되는 조건 하에서 상기 분할 구역은 비-염기쌍 버블 구조를 형성하고, 상기 버블 구조는 혼성화 특이성 측면에서 5'-고 Tm 특이성 구역이 3'-저 Tm 특이성 구역으로부터 분할되도록 하며, 이러한 특이성 분할은 DSO 전체 구조의 혼성화 특이성이 5'-고 Tm 특이성 구역과 3'-저 Tm 특이성 구역에 의해 이중적으로 결정도록 하고, 이는 결국 DSO 전체 구조의 혼성화 특이성을 향상시키며, 상기 호흡기 바이러스는 인플루엔자 바이러스, 인간 파라인플루엔자 바이러스, 인간 호흡기 신시티움 바이러스, 인간 메타뉴모바이러 스, 인간 코로나바이러스, 인간 리노바이러스 또는 아데노바이러스이며, 상기 타깃 서열은 상기 호흡기 바이러스의 지놈 서열이다.
- 제 1 항에 있어서, 상기 유니버설 염기는 디옥시이노신, 이노신, 7-디아자-2‘-디옥시이노신, 2-아자-2’-디옥시이노신, 2‘-OMe 이노신, 2'-F 이노신, 디옥시 3-니트로피롤, 3-니트로피롤, 2'-OMe 3-니트로피롤, 2'-F 3-니트로피롤, 1-(2’-디옥시-베타-D-리보푸라노실)-3-니트로피롤, 디옥시 5-니트로피롤, 5-니트로인돌, 2'-OMe 5-니트로인돌, 2'-F 5-니트로인돌, 디옥시 4-니트로벤즈이미다졸, 4-니트로벤즈이미다졸, 디옥시 4-아미노벤즈이미다졸, 4-아미노벤즈이미다졸, 디옥시 네불라린, 2'-F 네불라린, 2'-F 4-니트로벤즈이미다졸, PNA-5-인트로인돌, PNA-네불라린, PNA-이노신, PNA-4-니트로벤즈이미다졸, PNA-3-니트로피롤, 모르포리노-5-니트로인돌, 모르포리노-네불라린, 모르포리노-이노신, 모르포리노-4-니트로벤즈이미다졸, 모르포리노-3-니트로피롤, 포스포라미데이트-5-니트로인돌, 포스포라미데이트-네불라린, 포스포라미데이트-이노신, 포스포라미데이트-4-니트로벤즈이미다졸, 포스포라미데이트-3-니트로피롤, 2'-0-메톡시에틸이노신, 2'-0-메톡시에틸 네불라린, 2'-0-메톡시에틸 5-니트로인돌, 2'-0-메톡시에틸 4-니트로-벤즈이미다졸, 2'-0-메톡시에틸 3-니트로피롤 및 상기 염기의 조합으로 구성된 군으로부터 선택되는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 2 항에 있어서, 상기 유니버설 염기는 디옥시이노신, 이노신, 1-(2‘-디옥시-베타-D-리보푸라노실)-3-니트로피롤 또는 5-니트로인돌인 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 3 항에 있어서, 상기 유니버설 염기는 디옥시이노신인 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 분할 구역은 유니버설 염기를 가지는 연속된 뉴클레오타이드를 포함하는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 5'-고 Tm 특이성 구역의 길이는 3'-저 Tm 특이성 구역보다 긴 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 5'-고 Tm 특이성 구역은 15-40 뉴클레오타이드의 길이를 가지는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 3'-저 Tm 특이성 구역은 3-15 뉴클레오타이드의 길이를 가지는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 분할 구역은 3-10 뉴클레오타이드의 길이를 가지는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 5'-고 Tm 특이성 구역은 40-80℃의 Tm을 가지는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 3'-저 Tm 특이성 구역은 10-40℃의 Tm을 가지는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 분할 구역은 3-15℃의 Tm을 가지는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 분할 구역은 상기 호흡기 바이러스 지놈 타깃 서열에서 유전적 다양성을 보이는 서열과 혼성화되는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 5'-고 Tm 특이성 구역과 3'-저 Tm 특이성 구역은 상기 호흡기 바이러스 지놈의 보존성 서열과 혼성화되는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 호흡기 바이러스 지놈 타깃 서열은 서열목록 제1서 열 내지 제11서열로 구성된 군으로부터 선택되는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 제 1 항에 있어서, 상기 올리고뉴클레오타이드는 서열목록 제12서열 내지 제37서열로 구성된 군으로부터 선택되는 것을 특징으로 하는 호흡기 바이러스 핵산과 특이적으로 혼성화되는 올리고뉴클레오타이드.
- 서열목록 제12 및 13 서열, 서열목록 제 14 및 15 서열, 서열목록 제 16 및 17 서열, 서열목록 제 18 및 19 서열, 서열목록 제 20 및 21 서열, 서열목록 제 22 및 23 서열, 서열목록 제 24 및 25 서열, 서열목록 제 26 및 27 서열, 서열목록 제 28 및 29 서열, 서열목록 제 30 및 31 서열, 서열목록 제 32 및 33 서열, 서열목록 제 34 및 35 서열, 그리고 서열목록 제 36 및 제 37 서열로 구성된 군으로부터 선택되며, 호흡기 바이러스 핵산과 혼성화되는 올리고뉴클레오타이드 쌍 (pair).
- 상기 제 1 항 내지 제 16 항 중 어느 한 항의 올리고뉴클레오타이드, 또는 상기 제 17 항의 올리고뉴클레오타이드 쌍을 포함하는 호흡기 바이러스 검출용 키트.
- 상기 제 1 항 내지 제 16 항 중 어느 한 항의 올리고뉴클레오타이드, 또는 상기 제 17 항의 올리고뉴클레오타이드 쌍을 포함하는 호흡기 바이러스 증폭용 키트.
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SG168423A1 (en) * | 2009-07-13 | 2011-02-28 | Agency Science Tech & Res | Influenza detection method and kit therefor |
WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
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