CN1578841B - 退火控制引物及该退火控制引物的使用 - Google Patents

退火控制引物及该退火控制引物的使用 Download PDF

Info

Publication number
CN1578841B
CN1578841B CN028225090A CN02822509A CN1578841B CN 1578841 B CN1578841 B CN 1578841B CN 028225090 A CN028225090 A CN 028225090A CN 02822509 A CN02822509 A CN 02822509A CN 1578841 B CN1578841 B CN 1578841B
Authority
CN
China
Prior art keywords
primer
amplification
annealing
primers
cdna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN028225090A
Other languages
English (en)
Chinese (zh)
Other versions
CN1578841A (zh
Inventor
千锺润
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seegene Inc
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/KR2001/002133 external-priority patent/WO2003050304A1/en
Priority claimed from PCT/KR2002/000816 external-priority patent/WO2003093509A1/en
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of CN1578841A publication Critical patent/CN1578841A/zh
Application granted granted Critical
Publication of CN1578841B publication Critical patent/CN1578841B/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113—Differential Display Analysis [DDA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Application Of Or Painting With Fluid Materials (AREA)
  • Saccharide Compounds (AREA)
CN028225090A 2001-12-08 2002-09-19 退火控制引物及该退火控制引物的使用 Expired - Lifetime CN1578841B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/KR2001/002133 WO2003050304A1 (en) 2001-12-08 2001-12-08 Annealing control primer system for regulating primer annealing specificity and its applications
KRPCT/KR01/02133 2001-12-08
PCT/KR2002/000816 WO2003093509A1 (en) 2002-05-01 2002-05-01 Methods and compositions for improving specificity of pcr amplication
KRPCT/KR02/00816 2002-05-01
PCT/KR2002/001781 WO2003050305A1 (en) 2001-12-08 2002-09-19 Annealing control primer and its uses

Publications (2)

Publication Number Publication Date
CN1578841A CN1578841A (zh) 2005-02-09
CN1578841B true CN1578841B (zh) 2013-03-27

Family

ID=26625778

Family Applications (2)

Application Number Title Priority Date Filing Date
CN028225090A Expired - Lifetime CN1578841B (zh) 2001-12-08 2002-09-19 退火控制引物及该退火控制引物的使用
CNA028245016A Pending CN1602361A (zh) 2001-12-08 2002-11-04 杂交部分控制寡核苷酸及其应用

Family Applications After (1)

Application Number Title Priority Date Filing Date
CNA028245016A Pending CN1602361A (zh) 2001-12-08 2002-11-04 杂交部分控制寡核苷酸及其应用

Country Status (15)

Country Link
US (1) US20050164184A1 (enExample)
EP (2) EP1448793B1 (enExample)
JP (2) JP4263612B2 (enExample)
KR (2) KR100649165B1 (enExample)
CN (2) CN1578841B (enExample)
AT (1) ATE407224T1 (enExample)
AU (2) AU2002329104B2 (enExample)
BR (2) BRPI0214741B1 (enExample)
CA (2) CA2468754C (enExample)
DE (1) DE60228750D1 (enExample)
ES (1) ES2314093T3 (enExample)
IL (2) IL162317A0 (enExample)
NZ (2) NZ532531A (enExample)
RU (2) RU2004120771A (enExample)
WO (2) WO2003050305A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103421777A (zh) * 2013-09-11 2013-12-04 北京市农林科学院林业果树研究所 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用
US9587272B2 (en) 2011-05-09 2017-03-07 Fluidigm Corporation Probe based nucleic acid detection
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175749A1 (en) 2001-12-08 2003-09-18 Jong-Yoon Chun Annealing control primer and its uses
US8680063B2 (en) 2003-09-12 2014-03-25 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
DE602004011546T2 (de) * 2003-10-02 2008-05-29 Basf Se Verfahren zur sequenz-saturations-mutagenese (sesam)
WO2005045073A1 (en) * 2003-11-10 2005-05-19 Seegene, Inc. Method for amplifying unknown dna sequence adjacent to known sequence
WO2005083121A1 (en) * 2004-02-27 2005-09-09 Seegene, Inc. Method for amplifying members of a gene family
US7524631B2 (en) * 2005-02-02 2009-04-28 Patterson Bruce K HPV E6, E7 mRNA assay and methods of use thereof
RU2400538C2 (ru) * 2005-03-05 2010-09-27 Сиджен, Инк. Олигонуклеотид с двойственной специфичностью и способы, в которых он используется
WO2006095941A1 (en) 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
US7892795B2 (en) * 2005-08-02 2011-02-22 Focus Diagnostics, Inc. Methods and compositions for detecting BK virus
EP2239328A3 (en) 2005-08-18 2011-01-05 Alnylam Pharmaceuticals Inc. Methods and compositions for treating neurological disease
JP5137118B2 (ja) 2005-08-30 2013-02-06 国立大学法人高知大学 ユニバーサル塩基
KR20070052890A (ko) * 2005-11-18 2007-05-23 주식회사 씨젠 호흡기 바이러스 핵산 검출용 올리고뉴클레오타이드
KR100860619B1 (ko) * 2006-02-23 2008-09-29 주식회사 씨젠 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드
US8192940B2 (en) 2006-05-04 2012-06-05 Seegene, Inc. Method for amplifying unknown DNA sequence adjacent to known sequence
KR20080037128A (ko) * 2006-10-25 2008-04-30 주식회사 씨젠 뉴클레오타이드 변이 검출 방법
EP3095873B1 (en) * 2006-12-21 2018-04-18 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US7820389B2 (en) * 2007-01-10 2010-10-26 Geneohm Sciences, Inc. Inhibition of mismatch hybridization by a universal competitor DNA
KR100881924B1 (ko) * 2007-03-14 2009-02-04 주식회사 씨젠 레이블링된 프라이머를 이용한 뉴클레오타이드 변이 검출방법
WO2008143367A1 (en) * 2007-05-21 2008-11-27 Seegene, Inc. Haplotyping method by multiplex amplification
KR100987352B1 (ko) 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법
US9534255B2 (en) 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP5687414B2 (ja) 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
US20120142004A1 (en) * 2009-05-21 2012-06-07 Siemens Healthcare Diagnostics Inc. Universal Tags With Non-Natural Nucleobases
KR20210056458A (ko) 2011-05-04 2021-05-18 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
WO2013038534A1 (ja) * 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
JP5503021B2 (ja) * 2011-09-14 2014-05-28 日本碍子株式会社 標的核酸の検出方法
EP2839039B1 (en) * 2012-04-18 2017-06-07 F. Hoffmann-La Roche AG Hev assay
KR101525495B1 (ko) * 2013-11-15 2015-06-03 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
US20190032122A1 (en) * 2014-12-19 2019-01-31 Geco Aps Indel detection by amplicon analysis
KR101723207B1 (ko) * 2015-01-29 2017-04-05 (주)다이오진 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법
US20180073082A1 (en) * 2015-02-25 2018-03-15 Diogene Co., Ltd Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same
HUE044940T2 (hu) * 2016-01-27 2019-11-28 Devyser Holding Ab Kívánt nukleinsavrégiók szelektív amplifikálása egy célbavett szekvenciában
KR101961642B1 (ko) 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
WO2017209575A1 (en) * 2016-06-03 2017-12-07 Seegene, Inc. Evaluation of specificity of oligonucleotides
KR101856205B1 (ko) * 2017-11-16 2018-06-20 제노플랜코리아 주식회사 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법
CN110456034B (zh) * 2018-05-07 2022-07-22 上海市第十人民医院 一种循环肿瘤细胞的检测方法
CN109868309B (zh) * 2019-03-05 2022-11-11 苏州恩可医药科技有限公司 基于通用碱基替换插入的单链dna扩增方法
CN110592200B (zh) * 2019-09-25 2023-04-18 人和未来生物科技（长沙）有限公司 一种改善扩增特异性和均一性的多重pcr方法
CN111635930B (zh) * 2020-05-12 2023-10-24 中国农业科学院农业基因组研究所 一种高通量测序调取未知rna全长序列的方法
CN113832147B (zh) * 2021-09-08 2024-06-14 华南农业大学 一种高效的大片段dna合成与扩增的pcr引物、方法及应用
CN115976173B (zh) * 2022-12-21 2026-05-19 生工生物工程（上海）股份有限公司 一种用于扩增cDNA 5’末端的通用接头引物及5’RACE方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1312274A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人bcr蛋白10和编码这种多肽的多核苷酸

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2544292A (en) * 1991-09-11 1993-04-05 Medical Research Council Improvements in oligonucleotide primers and probes
US6197556B1 (en) * 1991-12-20 2001-03-06 The University Of Chicago Nucleic acid amplification using modular branched primers
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US20020042048A1 (en) * 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
US20020187470A1 (en) * 1998-06-24 2002-12-12 Casey Warren Michael Detection of single nucleotide polymorphisms
AU7733200A (en) * 1999-09-30 2001-04-30 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity
US6562572B1 (en) * 2000-09-27 2003-05-13 Mayo Foundation For Medical Education And Research Methods for amplifying nucleic acid sequences

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1312274A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人bcr蛋白10和编码这种多肽的多核苷酸

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9587272B2 (en) 2011-05-09 2017-03-07 Fluidigm Corporation Probe based nucleic acid detection
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
CN103421777A (zh) * 2013-09-11 2013-12-04 北京市农林科学院林业果树研究所 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用
CN103421777B (zh) * 2013-09-11 2015-05-13 北京市农林科学院林业果树研究所 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用

Also Published As

Publication number Publication date
KR20050028904A (ko) 2005-03-23
JP2005511096A (ja) 2005-04-28
CN1578841A (zh) 2005-02-09
RU2004120771A (ru) 2006-01-10
ATE407224T1 (de) 2008-09-15
EP1448793A1 (en) 2004-08-25
KR100557329B1 (ko) 2006-03-03
NZ533221A (en) 2006-01-27
AU2002329104B2 (en) 2005-08-18
CA2468754C (en) 2011-11-15
DE60228750D1 (de) 2008-10-16
ES2314093T3 (es) 2009-03-16
RU2004120769A (ru) 2005-04-20
CA2468754A1 (en) 2003-06-19
EP1448793B1 (en) 2008-09-03
US20050164184A1 (en) 2005-07-28
BRPI0214741B1 (pt) 2015-05-12
KR100649165B1 (ko) 2006-11-24
WO2003050305A1 (en) 2003-06-19
CA2469383A1 (en) 2003-06-19
KR20040070206A (ko) 2004-08-06
AU2002329104A1 (en) 2003-06-23
EP1448795A1 (en) 2004-08-25
JP4263612B2 (ja) 2009-05-13
NZ532531A (en) 2007-01-26
IL162317A (en) 2010-04-29
BR0214741A (pt) 2004-11-23
JP2005522190A (ja) 2005-07-28
IL162317A0 (en) 2005-11-20
CN1602361A (zh) 2005-03-30
AU2002348612A1 (en) 2003-06-23
WO2003050306A1 (en) 2003-06-19

Similar Documents

Publication Publication Date Title
JP4263612B2 (ja) アニーリング調節プライマーおよびその使用
US8124346B2 (en) Annealing control primer and its uses
JP2005511096A6 (ja) アニーリング調節プライマーおよびその使用
CN101778952B (zh) 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增
KR101243266B1 (ko) 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드
AU704625B2 (en) Method for characterizing nucleic acid molecules
KR100977186B1 (ko) 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중특이성 올리고뉴클레오타이드
JPH07500735A (ja) メッセンジャーｒｎａの同定、単離およびクローニング
US20030152925A1 (en) Annealing control primer system for regulating primer annealing specificity and its applications
WO2003093509A1 (en) Methods and compositions for improving specificity of pcr amplication
JPH10510981A (ja) ヌクレオチド配列を特性決定するための方法、装置及び組成物
KR101481734B1 (ko) 한국산 황기의 마이크로새틀라이트 마커 및 이를 증폭하기 위한 프라이머 세트
WO2001059101A1 (en) Method for amplifying full length single strand polynucleotide sequences
KR101249635B1 (ko) 양극성 장애 진단용 ｅｇｒ2 유전자 ｓｎｐ, 그를 포함하는 마이크로어레이 및 키트
HK1146094B (en) Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20130327