HUE044940T2 - Kívánt nukleinsavrégiók szelektív amplifikálása egy célbavett szekvenciában - Google Patents
Kívánt nukleinsavrégiók szelektív amplifikálása egy célbavett szekvenciábanInfo
- Publication number
- HUE044940T2 HUE044940T2 HUE17702824A HUE044940T2 HU E044940 T2 HUE044940 T2 HU E044940T2 HU E17702824 A HUE17702824 A HU E17702824A HU E044940 T2 HUE044940 T2 HU E044940T2
- Authority
- HU
- Hungary
- Prior art keywords
- nucleic acid
- target sequence
- desired nucleic
- selective amplification
- acid regions
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/159—Reduction of complexity, e.g. amplification of subsets, removing duplicated genomic regions
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16152951 | 2016-01-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HUE044940T2 true HUE044940T2 (hu) | 2019-11-28 |
Family
ID=55237575
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HUE17702824 HUE044940T2 (hu) | 2016-01-27 | 2017-01-27 | Kívánt nukleinsavrégiók szelektív amplifikálása egy célbavett szekvenciában |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10246740B2 (hu) |
| EP (1) | EP3408408B1 (hu) |
| CN (1) | CN108699594B (hu) |
| DK (1) | DK3408408T3 (hu) |
| ES (1) | ES2740109T3 (hu) |
| HU (1) | HUE044940T2 (hu) |
| PL (1) | PL3408408T3 (hu) |
| PT (1) | PT3408408T (hu) |
| TR (1) | TR201908099T4 (hu) |
| WO (1) | WO2017129756A1 (hu) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117778578B (zh) * | 2023-12-29 | 2024-10-15 | 深圳海普洛斯医学检验实验室 | 一种基于高通量测序检测目标基因甲基化程度的引物组合物及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| US5780233A (en) * | 1996-06-06 | 1998-07-14 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
| AUPQ008799A0 (en) * | 1999-04-30 | 1999-05-27 | Tillett, Daniel | Genome sequencing |
| BR0214741A (pt) * | 2001-12-08 | 2004-11-23 | Seegene Inc | Iniciador de controle de anelamento para melhorar a especificidade de anelamento na amplificação de ácido nucléico, kit, métodos para amplificar uma sequência de ácido nucléico de um dna ou uma mistura de ácidos nucléicos, e para amplificar seletivamente uma sequência de ácido nucléico alvo de um dna ou uma mistura de ácidos nucléicos, e uma sequência de ácido nucléico alvo a partir de um mrna, método para detectar a complementaridade de dna ao mrna diferencialmente expresso em duas ou mais amostras de ácido nucléico, métodos para amplificar rapidamente um fragmento de cdna e dna alvo, e para amplificar uma população de cdnas de filamento duplo de comprimento completo complementar aos mrnas, e cdnas de filamento duplo enriquecidos em 5' complementares aos mrnas, método para amplificar mais do que uma sequência de nucleotìdeo alvo simultaneamente, métodos para produzir uma impressão digital de dna de gdna, e de rna de uma amostra de mrna, método para identificar segmentos de homologia conservada em uma famìlia de multigene a partir de uma amostra de mrna, método para a mutagênese em um ácido nucléico alvo, e, uso do iniciador |
| WO2008033442A2 (en) * | 2006-09-12 | 2008-03-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for performing low background multiplex nucleic acid amplification reactions |
| CA2978709C (en) | 2015-03-06 | 2024-06-04 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
| US10221448B2 (en) | 2015-03-06 | 2019-03-05 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
| AU2016289369B2 (en) | 2015-07-07 | 2020-07-02 | Pillar Biosciences Inc. | Method for reducing primer-dimer amplification |
-
2017
- 2017-01-27 WO PCT/EP2017/051793 patent/WO2017129756A1/en active Application Filing
- 2017-01-27 EP EP17702824.8A patent/EP3408408B1/en active Active
- 2017-01-27 PL PL17702824T patent/PL3408408T3/pl unknown
- 2017-01-27 PT PT17702824T patent/PT3408408T/pt unknown
- 2017-01-27 ES ES17702824T patent/ES2740109T3/es active Active
- 2017-01-27 CN CN201780007649.8A patent/CN108699594B/zh active Active
- 2017-01-27 DK DK17702824.8T patent/DK3408408T3/da active
- 2017-01-27 TR TR2019/08099T patent/TR201908099T4/tr unknown
- 2017-01-27 HU HUE17702824 patent/HUE044940T2/hu unknown
- 2017-01-27 US US16/063,227 patent/US10246740B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US10246740B2 (en) | 2019-04-02 |
| EP3408408A1 (en) | 2018-12-05 |
| CN108699594B (zh) | 2020-03-27 |
| EP3408408B1 (en) | 2019-05-15 |
| WO2017129756A1 (en) | 2017-08-03 |
| PT3408408T (pt) | 2019-07-09 |
| US20180371528A1 (en) | 2018-12-27 |
| TR201908099T4 (tr) | 2019-06-21 |
| CN108699594A (zh) | 2018-10-23 |
| PL3408408T3 (pl) | 2019-09-30 |
| DK3408408T3 (da) | 2019-07-22 |
| ES2740109T3 (es) | 2020-02-05 |
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