JP4263612B2 - アニーリング調節プライマーおよびその使用 - Google Patents

アニーリング調節プライマーおよびその使用 Download PDF

Info

Publication number
JP4263612B2
JP4263612B2 JP2003551326A JP2003551326A JP4263612B2 JP 4263612 B2 JP4263612 B2 JP 4263612B2 JP 2003551326 A JP2003551326 A JP 2003551326A JP 2003551326 A JP2003551326 A JP 2003551326A JP 4263612 B2 JP4263612 B2 JP 4263612B2
Authority
JP
Japan
Prior art keywords
primer
annealing
sequence
amplification
cdna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2003551326A
Other languages
English (en)
Japanese (ja)
Other versions
JP2005511096A6 (ja
JP2005511096A5 (enExample
JP2005511096A (ja
Inventor
チョン，ゾン−ユン
Original Assignee
シーゼン インコーポレーイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/KR2002/000816 external-priority patent/WO2003093509A1/en
Application filed by シーゼン インコーポレーイテッド filed Critical シーゼン インコーポレーイテッド
Priority claimed from PCT/KR2002/001781 external-priority patent/WO2003050305A1/en
Publication of JP2005511096A publication Critical patent/JP2005511096A/ja
Publication of JP2005511096A6 publication Critical patent/JP2005511096A6/ja
Publication of JP2005511096A5 publication Critical patent/JP2005511096A5/ja
Application granted granted Critical
Publication of JP4263612B2 publication Critical patent/JP4263612B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113—Differential Display Analysis [DDA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2003551326A 2001-12-08 2002-09-19 アニーリング調節プライマーおよびその使用 Expired - Lifetime JP4263612B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/KR2001/002133 WO2003050304A1 (en) 2001-12-08 2001-12-08 Annealing control primer system for regulating primer annealing specificity and its applications
KRPCT/KR01/02133 2001-12-08
KRPCT/KR02/00816 2002-05-01
PCT/KR2002/000816 WO2003093509A1 (en) 2002-05-01 2002-05-01 Methods and compositions for improving specificity of pcr amplication
PCT/KR2002/001781 WO2003050305A1 (en) 2001-12-08 2002-09-19 Annealing control primer and its uses

Publications (4)

Publication Number Publication Date
JP2005511096A JP2005511096A (ja) 2005-04-28
JP2005511096A6 JP2005511096A6 (ja) 2005-08-04
JP2005511096A5 JP2005511096A5 (enExample) 2005-12-22
JP4263612B2 true JP4263612B2 (ja) 2009-05-13

Family

ID=19198489

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2003551326A Expired - Lifetime JP4263612B2 (ja) 2001-12-08 2002-09-19 アニーリング調節プライマーおよびその使用
JP2003551327A Pending JP2005522190A (ja) 2001-12-08 2002-11-04 ハイブリダイゼーション部分調節オリゴヌクレオチド及びその用途

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2003551327A Pending JP2005522190A (ja) 2001-12-08 2002-11-04 ハイブリダイゼーション部分調節オリゴヌクレオチド及びその用途

Country Status (9)

Country Link
US (1) US20030152925A1 (enExample)
JP (2) JP4263612B2 (enExample)
KR (1) KR100649165B1 (enExample)
AU (1) AU2002222737A1 (enExample)
CA (1) CA2469383A1 (enExample)
IL (1) IL162317A0 (enExample)
NZ (1) NZ533221A (enExample)
RU (1) RU2004120769A (enExample)
WO (1) WO2003050304A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
WO2007026485A1 (ja) * 2005-08-30 2007-03-08 Inter-University Research Institute Corporation National Institutes Of Natural Sciences ユニバーサル塩基
EP2479291A1 (en) * 2005-10-27 2012-07-25 Life Technologies Corporation Nucleic acid amplification using non-random primers
EP1957645B1 (en) 2005-12-06 2010-11-17 Ambion Inc. Reverse transcription primers and methods of design
KR100860619B1 (ko) * 2006-02-23 2008-09-29 주식회사 씨젠 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드
ES2400805T3 (es) * 2006-05-04 2013-04-12 Seegene, Inc. Método para amplificar una secuencia de ADN desconocida adyacente a una secuencia conocida
WO2008080029A2 (en) 2006-12-21 2008-07-03 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP1978104A1 (en) * 2007-04-03 2008-10-08 Freie Universität Berlin A method for the quantitative analysis of RNA molecules in a sample
KR100987352B1 (ko) 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP5687414B2 (ja) 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
EP3150619B1 (en) * 2009-05-21 2019-09-18 Siemens Healthcare Diagnostics Inc. Universal tags with non-natural nucleobases
EP2449132B1 (en) 2009-07-01 2015-05-13 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2011032053A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
ES2736977T3 (es) * 2011-09-14 2020-01-09 Ngk Insulators Ltd Método para detectar ácido nucleico diana
CA2862373C (en) 2012-04-18 2016-07-19 F. Hoffmann-La Roche Ag Hev assay
KR101525495B1 (ko) * 2013-11-15 2015-06-03 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
KR101723207B1 (ko) * 2015-01-29 2017-04-05 (주)다이오진 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법
US20180073082A1 (en) * 2015-02-25 2018-03-15 Diogene Co., Ltd Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
KR101961642B1 (ko) * 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
CN111315895A (zh) * 2017-09-14 2020-06-19 豪夫迈·罗氏有限公司 用于产生环状单链dna文库的新型方法
KR101856205B1 (ko) * 2017-11-16 2018-06-20 제노플랜코리아 주식회사 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2544292A (en) * 1991-09-11 1993-04-05 Medical Research Council Improvements in oligonucleotide primers and probes
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
AU7733200A (en) * 1999-09-30 2001-04-30 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity

Also Published As

Publication number Publication date
WO2003050304A1 (en) 2003-06-19
NZ533221A (en) 2006-01-27
AU2002222737A1 (en) 2003-06-23
IL162317A0 (en) 2005-11-20
JP2005522190A (ja) 2005-07-28
RU2004120769A (ru) 2005-04-20
US20030152925A1 (en) 2003-08-14
KR20050028904A (ko) 2005-03-23
CA2469383A1 (en) 2003-06-19
JP2005511096A (ja) 2005-04-28
KR100649165B1 (ko) 2006-11-24

Similar Documents

Publication Publication Date Title
JP4263612B2 (ja) アニーリング調節プライマーおよびその使用
US10138518B2 (en) Annealing control primer and its uses
EP1448793B1 (en) Annealing control primer and its uses
JP2005511096A6 (ja) アニーリング調節プライマーおよびその使用
DK2365078T3 (en) Methods using the dual specificity oligonucleotide and dual specificity oligonucleotide
EP1856257B1 (en) Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
JP5938348B2 (ja) 反応特異性の増加のための非標準塩基を含む増幅プライマー
WO2003093509A1 (en) Methods and compositions for improving specificity of pcr amplication

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20071113

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20080204

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20080212

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20080411

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20080418

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080509

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20080610

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20080904

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20080911

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20081210

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20090113

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20090212

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120220

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Ref document number: 4263612

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130220

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140220

Year of fee payment: 5

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term