KR100649165B1 - 어닐링 조절 프라이머 및 그의 용도 - Google Patents

어닐링 조절 프라이머 및 그의 용도 Download PDF

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Publication number
KR100649165B1
KR100649165B1 KR1020047008441A KR20047008441A KR100649165B1 KR 100649165 B1 KR100649165 B1 KR 100649165B1 KR 1020047008441 A KR1020047008441 A KR 1020047008441A KR 20047008441 A KR20047008441 A KR 20047008441A KR 100649165 B1 KR100649165 B1 KR 100649165B1
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primer
annealing
amplification
primary
primers
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KR20050028904A (ko
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천종윤
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주식회사 씨젠
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Priority claimed from PCT/KR2001/002133 external-priority patent/WO2003050304A1/en
Priority claimed from PCT/KR2002/000816 external-priority patent/WO2003093509A1/en
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113—Differential Display Analysis [DDA]

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020047008441A 2001-12-08 2002-09-19 어닐링 조절 프라이머 및 그의 용도 Expired - Lifetime KR100649165B1 (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
WOPCT/KR01/02133 2001-12-08
PCT/KR2001/002133 WO2003050304A1 (en) 2001-12-08 2001-12-08 Annealing control primer system for regulating primer annealing specificity and its applications
WOPCT/KR2001/002133 2001-12-08
PCT/KR2002/000816 WO2003093509A1 (en) 2002-05-01 2002-05-01 Methods and compositions for improving specificity of pcr amplication
WOPCT/KR02/00816 2002-05-01
WOPCT/KR2002/000816 2002-05-01

Publications (2)

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KR20050028904A KR20050028904A (ko) 2005-03-23
KR100649165B1 true KR100649165B1 (ko) 2006-11-24

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KR1020047008441A Expired - Lifetime KR100649165B1 (ko) 2001-12-08 2002-09-19 어닐링 조절 프라이머 및 그의 용도
KR1020047008678A Expired - Lifetime KR100557329B1 (ko) 2001-12-08 2002-11-04 혼성화 부위 조절 올리고뉴클레오타이드 및 그의 용도

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US (1) US20050164184A1 (enExample)
EP (2) EP1448793B1 (enExample)
JP (2) JP4263612B2 (enExample)
KR (2) KR100649165B1 (enExample)
CN (2) CN1578841B (enExample)
AT (1) ATE407224T1 (enExample)
AU (2) AU2002329104B2 (enExample)
BR (2) BRPI0214741B1 (enExample)
CA (2) CA2468754C (enExample)
DE (1) DE60228750D1 (enExample)
ES (1) ES2314093T3 (enExample)
IL (2) IL162317A0 (enExample)
NZ (2) NZ532531A (enExample)
RU (2) RU2004120771A (enExample)
WO (2) WO2003050305A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100987352B1 (ko) 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법
KR101525495B1 (ko) * 2013-11-15 2015-06-03 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
WO2016137031A1 (ko) * 2015-02-25 2016-09-01 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법

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US8680063B2 (en) 2003-09-12 2014-03-25 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
DE602004011546T2 (de) * 2003-10-02 2008-05-29 Basf Se Verfahren zur sequenz-saturations-mutagenese (sesam)
WO2005045073A1 (en) * 2003-11-10 2005-05-19 Seegene, Inc. Method for amplifying unknown dna sequence adjacent to known sequence
WO2005083121A1 (en) * 2004-02-27 2005-09-09 Seegene, Inc. Method for amplifying members of a gene family
US7524631B2 (en) * 2005-02-02 2009-04-28 Patterson Bruce K HPV E6, E7 mRNA assay and methods of use thereof
RU2400538C2 (ru) * 2005-03-05 2010-09-27 Сиджен, Инк. Олигонуклеотид с двойственной специфичностью и способы, в которых он используется
WO2006095941A1 (en) 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
US7892795B2 (en) * 2005-08-02 2011-02-22 Focus Diagnostics, Inc. Methods and compositions for detecting BK virus
EP2239328A3 (en) 2005-08-18 2011-01-05 Alnylam Pharmaceuticals Inc. Methods and compositions for treating neurological disease
JP5137118B2 (ja) 2005-08-30 2013-02-06 国立大学法人高知大学 ユニバーサル塩基
KR20070052890A (ko) * 2005-11-18 2007-05-23 주식회사 씨젠 호흡기 바이러스 핵산 검출용 올리고뉴클레오타이드
KR100860619B1 (ko) * 2006-02-23 2008-09-29 주식회사 씨젠 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드
US8192940B2 (en) 2006-05-04 2012-06-05 Seegene, Inc. Method for amplifying unknown DNA sequence adjacent to known sequence
KR20080037128A (ko) * 2006-10-25 2008-04-30 주식회사 씨젠 뉴클레오타이드 변이 검출 방법
EP3095873B1 (en) * 2006-12-21 2018-04-18 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US7820389B2 (en) * 2007-01-10 2010-10-26 Geneohm Sciences, Inc. Inhibition of mismatch hybridization by a universal competitor DNA
KR100881924B1 (ko) * 2007-03-14 2009-02-04 주식회사 씨젠 레이블링된 프라이머를 이용한 뉴클레오타이드 변이 검출방법
WO2008143367A1 (en) * 2007-05-21 2008-11-27 Seegene, Inc. Haplotyping method by multiplex amplification
US9534255B2 (en) 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
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KR20210056458A (ko) 2011-05-04 2021-05-18 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
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WO2013038534A1 (ja) * 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
JP5503021B2 (ja) * 2011-09-14 2014-05-28 日本碍子株式会社 標的核酸の検出方法
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CN103421777B (zh) * 2013-09-11 2015-05-13 北京市农林科学院林业果树研究所 无需pcr仪的侧翼核酸序列快速准确扩增方法及应用
US20190032122A1 (en) * 2014-12-19 2019-01-31 Geco Aps Indel detection by amplicon analysis
KR101723207B1 (ko) * 2015-01-29 2017-04-05 (주)다이오진 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법
HUE044940T2 (hu) * 2016-01-27 2019-11-28 Devyser Holding Ab Kívánt nukleinsavrégiók szelektív amplifikálása egy célbavett szekvenciában
KR101961642B1 (ko) 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
WO2017209575A1 (en) * 2016-06-03 2017-12-07 Seegene, Inc. Evaluation of specificity of oligonucleotides
KR101856205B1 (ko) * 2017-11-16 2018-06-20 제노플랜코리아 주식회사 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법
CN110456034B (zh) * 2018-05-07 2022-07-22 上海市第十人民医院 一种循环肿瘤细胞的检测方法
CN109868309B (zh) * 2019-03-05 2022-11-11 苏州恩可医药科技有限公司 基于通用碱基替换插入的单链dna扩增方法
CN110592200B (zh) * 2019-09-25 2023-04-18 人和未来生物科技（长沙）有限公司 一种改善扩增特异性和均一性的多重pcr方法
CN111635930B (zh) * 2020-05-12 2023-10-24 中国农业科学院农业基因组研究所 一种高通量测序调取未知rna全长序列的方法
CN113832147B (zh) * 2021-09-08 2024-06-14 华南农业大学 一种高效的大片段dna合成与扩增的pcr引物、方法及应用
CN115976173B (zh) * 2022-12-21 2026-05-19 生工生物工程（上海）股份有限公司 一种用于扩增cDNA 5’末端的通用接头引物及5’RACE方法

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100987352B1 (ko) 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법
KR101525495B1 (ko) * 2013-11-15 2015-06-03 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
WO2016137031A1 (ko) * 2015-02-25 2016-09-01 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
CN107075576A (zh) * 2015-02-25 2017-08-18 精确诊断有限公司 哑铃结构寡核苷酸、包含其的核酸扩增用引物及利用该引物的核酸扩增方法

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KR20050028904A (ko) 2005-03-23
JP2005511096A (ja) 2005-04-28
CN1578841A (zh) 2005-02-09
RU2004120771A (ru) 2006-01-10
ATE407224T1 (de) 2008-09-15
CN1578841B (zh) 2013-03-27
EP1448793A1 (en) 2004-08-25
KR100557329B1 (ko) 2006-03-03
NZ533221A (en) 2006-01-27
AU2002329104B2 (en) 2005-08-18
CA2468754C (en) 2011-11-15
DE60228750D1 (de) 2008-10-16
ES2314093T3 (es) 2009-03-16
RU2004120769A (ru) 2005-04-20
CA2468754A1 (en) 2003-06-19
EP1448793B1 (en) 2008-09-03
US20050164184A1 (en) 2005-07-28
BRPI0214741B1 (pt) 2015-05-12
WO2003050305A1 (en) 2003-06-19
CA2469383A1 (en) 2003-06-19
KR20040070206A (ko) 2004-08-06
AU2002329104A1 (en) 2003-06-23
EP1448795A1 (en) 2004-08-25
JP4263612B2 (ja) 2009-05-13
NZ532531A (en) 2007-01-26
IL162317A (en) 2010-04-29
BR0214741A (pt) 2004-11-23
JP2005522190A (ja) 2005-07-28
IL162317A0 (en) 2005-11-20
CN1602361A (zh) 2005-03-30
AU2002348612A1 (en) 2003-06-23
WO2003050306A1 (en) 2003-06-19

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