KR100649165B1 - 어닐링 조절 프라이머 및 그의 용도 - Google Patents
어닐링 조절 프라이머 및 그의 용도 Download PDFInfo
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- KR100649165B1 KR100649165B1 KR1020047008441A KR20047008441A KR100649165B1 KR 100649165 B1 KR100649165 B1 KR 100649165B1 KR 1020047008441 A KR1020047008441 A KR 1020047008441A KR 20047008441 A KR20047008441 A KR 20047008441A KR 100649165 B1 KR100649165 B1 KR 100649165B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
- C12Q2539/10—The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
- C12Q2539/113—Differential Display Analysis [DDA]
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| WOPCT/KR2001/002133 | 2001-12-08 | ||
| WOPCT/KR01/02133 | 2001-12-08 | ||
| PCT/KR2001/002133 WO2003050304A1 (en) | 2001-12-08 | 2001-12-08 | Annealing control primer system for regulating primer annealing specificity and its applications |
| PCT/KR2002/000816 WO2003093509A1 (en) | 2002-05-01 | 2002-05-01 | Methods and compositions for improving specificity of pcr amplication |
| WOPCT/KR2002/000816 | 2002-05-01 | ||
| WOPCT/KR02/00816 | 2002-05-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20050028904A KR20050028904A (ko) | 2005-03-23 |
| KR100649165B1 true KR100649165B1 (ko) | 2006-11-24 |
Family
ID=19198489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020047008441A Expired - Lifetime KR100649165B1 (ko) | 2001-12-08 | 2002-09-19 | 어닐링 조절 프라이머 및 그의 용도 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20030152925A1 (enExample) |
| JP (2) | JP4263612B2 (enExample) |
| KR (1) | KR100649165B1 (enExample) |
| AU (1) | AU2002222737A1 (enExample) |
| CA (1) | CA2469383A1 (enExample) |
| IL (1) | IL162317A0 (enExample) |
| NZ (1) | NZ533221A (enExample) |
| RU (1) | RU2004120769A (enExample) |
| WO (1) | WO2003050304A1 (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100987352B1 (ko) | 2008-04-15 | 2010-10-12 | 주식회사 인트론바이오테크놀로지 | 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법 |
| KR101525495B1 (ko) * | 2013-11-15 | 2015-06-03 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
| WO2016137031A1 (ko) * | 2015-02-25 | 2016-09-01 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1280422C (zh) * | 2004-08-26 | 2006-10-18 | 北京博奥生物芯片有限责任公司 | 一种不对称pcr扩增方法及其应用 |
| US20060188910A1 (en) * | 2005-02-18 | 2006-08-24 | Applera Corporation | Compositions, methods, and kits for analyzing DNA methylation |
| WO2007026485A1 (ja) * | 2005-08-30 | 2007-03-08 | Inter-University Research Institute Corporation National Institutes Of Natural Sciences | ユニバーサル塩基 |
| EP2479291A1 (en) * | 2005-10-27 | 2012-07-25 | Life Technologies Corporation | Nucleic acid amplification using non-random primers |
| EP1957645B1 (en) | 2005-12-06 | 2010-11-17 | Ambion Inc. | Reverse transcription primers and methods of design |
| KR100860619B1 (ko) * | 2006-02-23 | 2008-09-29 | 주식회사 씨젠 | 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드 |
| ES2400805T3 (es) * | 2006-05-04 | 2013-04-12 | Seegene, Inc. | Método para amplificar una secuencia de ADN desconocida adyacente a una secuencia conocida |
| WO2008080029A2 (en) | 2006-12-21 | 2008-07-03 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| EP1978104A1 (en) * | 2007-04-03 | 2008-10-08 | Freie Universität Berlin | A method for the quantitative analysis of RNA molecules in a sample |
| JP2012511927A (ja) | 2008-12-17 | 2012-05-31 | ライフ テクノロジーズ コーポレーション | 対立遺伝子変種を検出するための方法、組成物、およびキット |
| EP3249053A1 (en) | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| JP5687414B2 (ja) | 2009-04-20 | 2015-03-18 | オリンパス株式会社 | 多型の識別方法 |
| EP3150619B1 (en) * | 2009-05-21 | 2019-09-18 | Siemens Healthcare Diagnostics Inc. | Universal tags with non-natural nucleobases |
| EP2449132B1 (en) | 2009-07-01 | 2015-05-13 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| WO2011032053A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
| ES2736977T3 (es) * | 2011-09-14 | 2020-01-09 | Ngk Insulators Ltd | Método para detectar ácido nucleico diana |
| CA2862373C (en) | 2012-04-18 | 2016-07-19 | F. Hoffmann-La Roche Ag | Hev assay |
| KR101723207B1 (ko) * | 2015-01-29 | 2017-04-05 | (주)다이오진 | 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법 |
| CN104845967B (zh) * | 2015-04-15 | 2020-12-11 | 苏州新海生物科技股份有限公司 | 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用 |
| US10344336B2 (en) | 2015-06-09 | 2019-07-09 | Life Technologies Corporation | Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging |
| KR101961642B1 (ko) * | 2016-04-25 | 2019-03-25 | (주)진매트릭스 | 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물 |
| CN111315895A (zh) * | 2017-09-14 | 2020-06-19 | 豪夫迈·罗氏有限公司 | 用于产生环状单链dna文库的新型方法 |
| KR101856205B1 (ko) * | 2017-11-16 | 2018-06-20 | 제노플랜코리아 주식회사 | 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2544292A (en) * | 1991-09-11 | 1993-04-05 | Medical Research Council | Improvements in oligonucleotide primers and probes |
| WO1993014217A1 (en) * | 1992-01-10 | 1993-07-22 | Life Technologies, Inc. | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules |
| AU7733200A (en) * | 1999-09-30 | 2001-04-30 | Qiagen Genomics, Inc. | Compositions and methods for reducing oligonucleotide hybridization and priming specificity |
-
2001
- 2001-12-08 WO PCT/KR2001/002133 patent/WO2003050304A1/en not_active Ceased
- 2001-12-08 AU AU2002222737A patent/AU2002222737A1/en not_active Abandoned
- 2001-12-14 US US10/014,496 patent/US20030152925A1/en not_active Abandoned
-
2002
- 2002-09-19 JP JP2003551326A patent/JP4263612B2/ja not_active Expired - Lifetime
- 2002-09-19 IL IL16231702A patent/IL162317A0/xx unknown
- 2002-09-19 KR KR1020047008441A patent/KR100649165B1/ko not_active Expired - Lifetime
- 2002-11-04 CA CA002469383A patent/CA2469383A1/en not_active Abandoned
- 2002-11-04 NZ NZ533221A patent/NZ533221A/en unknown
- 2002-11-04 RU RU2004120769/13A patent/RU2004120769A/ru not_active Application Discontinuation
- 2002-11-04 JP JP2003551327A patent/JP2005522190A/ja active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100987352B1 (ko) | 2008-04-15 | 2010-10-12 | 주식회사 인트론바이오테크놀로지 | 비특이 증폭을 감소시킬 수 있는 ｐｃｒ용 프라이머 및이를 이용한 ｐｃｒ 방법 |
| KR101525495B1 (ko) * | 2013-11-15 | 2015-06-03 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
| WO2016137031A1 (ko) * | 2015-02-25 | 2016-09-01 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
| CN107075576A (zh) * | 2015-02-25 | 2017-08-18 | 精确诊断有限公司 | 哑铃结构寡核苷酸、包含其的核酸扩增用引物及利用该引物的核酸扩增方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003050304A1 (en) | 2003-06-19 |
| NZ533221A (en) | 2006-01-27 |
| AU2002222737A1 (en) | 2003-06-23 |
| IL162317A0 (en) | 2005-11-20 |
| JP2005522190A (ja) | 2005-07-28 |
| RU2004120769A (ru) | 2005-04-20 |
| US20030152925A1 (en) | 2003-08-14 |
| KR20050028904A (ko) | 2005-03-23 |
| JP4263612B2 (ja) | 2009-05-13 |
| CA2469383A1 (en) | 2003-06-19 |
| JP2005511096A (ja) | 2005-04-28 |
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