AU2002222737A1 - Annealing control primer system for regulating primer annealing specificity and its applications - Google Patents

Annealing control primer system for regulating primer annealing specificity and its applications

Info

Publication number
AU2002222737A1
AU2002222737A1 AU2002222737A AU2002222737A AU2002222737A1 AU 2002222737 A1 AU2002222737 A1 AU 2002222737A1 AU 2002222737 A AU2002222737 A AU 2002222737A AU 2002222737 A AU2002222737 A AU 2002222737A AU 2002222737 A1 AU2002222737 A1 AU 2002222737A1
Authority
AU
Australia
Prior art keywords
annealing
primer
applications
regulating
specificity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002222737A
Other languages
English (en)
Inventor
Jong Yoon Chun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seegene Inc
Original Assignee
Seegene Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seegene Inc filed Critical Seegene Inc
Publication of AU2002222737A1 publication Critical patent/AU2002222737A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/101Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/155Modifications characterised by incorporating/generating a new priming site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2539/00Reactions characterised by analysis of gene expression or genome comparison
    • C12Q2539/10The purpose being sequence identification by analysis of gene expression or genome comparison characterised by
    • C12Q2539/113Differential Display Analysis [DDA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2002222737A 2001-12-08 2001-12-08 Annealing control primer system for regulating primer annealing specificity and its applications Abandoned AU2002222737A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2001/002133 WO2003050304A1 (en) 2001-12-08 2001-12-08 Annealing control primer system for regulating primer annealing specificity and its applications

Publications (1)

Publication Number Publication Date
AU2002222737A1 true AU2002222737A1 (en) 2003-06-23

Family

ID=19198489

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002222737A Abandoned AU2002222737A1 (en) 2001-12-08 2001-12-08 Annealing control primer system for regulating primer annealing specificity and its applications

Country Status (9)

Country Link
US (1) US20030152925A1 (enExample)
JP (2) JP4263612B2 (enExample)
KR (1) KR100649165B1 (enExample)
AU (1) AU2002222737A1 (enExample)
CA (1) CA2469383A1 (enExample)
IL (1) IL162317A0 (enExample)
NZ (1) NZ533221A (enExample)
RU (1) RU2004120769A (enExample)
WO (1) WO2003050304A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
WO2007026485A1 (ja) * 2005-08-30 2007-03-08 Inter-University Research Institute Corporation National Institutes Of Natural Sciences ユニバーサル塩基
EP2479291A1 (en) * 2005-10-27 2012-07-25 Life Technologies Corporation Nucleic acid amplification using non-random primers
EP1957645B1 (en) 2005-12-06 2010-11-17 Ambion Inc. Reverse transcription primers and methods of design
KR100860619B1 (ko) * 2006-02-23 2008-09-29 주식회사 씨젠 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드
ES2400805T3 (es) * 2006-05-04 2013-04-12 Seegene, Inc. Método para amplificar una secuencia de ADN desconocida adyacente a una secuencia conocida
WO2008080029A2 (en) 2006-12-21 2008-07-03 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP1978104A1 (en) * 2007-04-03 2008-10-08 Freie Universität Berlin A method for the quantitative analysis of RNA molecules in a sample
KR100987352B1 (ko) 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 pcr용 프라이머 및이를 이용한 pcr 방법
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP5687414B2 (ja) 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
EP3150619B1 (en) * 2009-05-21 2019-09-18 Siemens Healthcare Diagnostics Inc. Universal tags with non-natural nucleobases
EP2449132B1 (en) 2009-07-01 2015-05-13 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2011032053A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
ES2736977T3 (es) * 2011-09-14 2020-01-09 Ngk Insulators Ltd Método para detectar ácido nucleico diana
CA2862373C (en) 2012-04-18 2016-07-19 F. Hoffmann-La Roche Ag Hev assay
KR101525495B1 (ko) * 2013-11-15 2015-06-03 (주)다이오진 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법
KR101723207B1 (ko) * 2015-01-29 2017-04-05 (주)다이오진 덤벨 구조의 올리고뉴클레오티드 및 이를 이용한 유전자 변이 검출 방법
US20180073082A1 (en) * 2015-02-25 2018-03-15 Diogene Co., Ltd Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same
CN104845967B (zh) * 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
KR101961642B1 (ko) * 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
CN111315895A (zh) * 2017-09-14 2020-06-19 豪夫迈·罗氏有限公司 用于产生环状单链dna文库的新型方法
KR101856205B1 (ko) * 2017-11-16 2018-06-20 제노플랜코리아 주식회사 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2544292A (en) * 1991-09-11 1993-04-05 Medical Research Council Improvements in oligonucleotide primers and probes
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
AU7733200A (en) * 1999-09-30 2001-04-30 Qiagen Genomics, Inc. Compositions and methods for reducing oligonucleotide hybridization and priming specificity

Also Published As

Publication number Publication date
WO2003050304A1 (en) 2003-06-19
NZ533221A (en) 2006-01-27
IL162317A0 (en) 2005-11-20
JP2005522190A (ja) 2005-07-28
RU2004120769A (ru) 2005-04-20
US20030152925A1 (en) 2003-08-14
KR20050028904A (ko) 2005-03-23
JP4263612B2 (ja) 2009-05-13
CA2469383A1 (en) 2003-06-19
JP2005511096A (ja) 2005-04-28
KR100649165B1 (ko) 2006-11-24

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase