JP2002529094A5 - - Google Patents

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Publication number
JP2002529094A5
JP2002529094A5 JP2000581219A JP2000581219A JP2002529094A5 JP 2002529094 A5 JP2002529094 A5 JP 2002529094A5 JP 2000581219 A JP2000581219 A JP 2000581219A JP 2000581219 A JP2000581219 A JP 2000581219A JP 2002529094 A5 JP2002529094 A5 JP 2002529094A5
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hybridization
sds
present
buffer
ssc
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JP2002529094A (ja
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Priority claimed from PCT/EP1999/008506 external-priority patent/WO2000028052A2/en
Publication of JP2002529094A publication Critical patent/JP2002529094A/ja
Publication of JP2002529094A5 publication Critical patent/JP2002529094A5/ja
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細胞のプラスチドに存在し、本発明の上記核酸分子とハイブリダイズする、特にコメのタンパク質をコードする核酸分子、またはそれらの相補鎖も、本発明の主題である。本発明において、「ハイブリダイゼーション」という用語は従来のハイブリダイゼーション条件下でのハイブリダイゼーションを意味し、サムブルックら（Sambrook）、「分子クローニング、実験マニュアル（Molecular Cloning, A Laboratory Manual）」第２版（1989）、Cold Spring Harbor Laboratory Press、コールドスプリングハーバー、ニューヨーク）が記述したストリンジェントな条件下が好ましい。より好ましくは、ハイブリダイゼーションは以下の条件下で生じる：
ハイブリダイゼーション緩衝液：２×SSC；10×デンハード溶液（フィコール400＋PEG＋BSA；比１：１：１）；0.1％SDS；５ mM EDTA；50 mM Na2HPO4；250μg/mlニシン精子DNA；50μg/ml tRNA；または
0.25 M リン酸ナトリウム緩衝液pH 7.2
1 mM EDTA
7％ SDS
ハイブリダイゼーション温度 T＝65〜68℃
洗浄緩衝液： 0.2×SSC；0.1％SDS
洗浄温度： T＝65〜68℃
JP2000581219A 1998-11-09 1999-11-05 コメ由来核酸分子および改変デンプンの生産のためのその使用 Withdrawn JP2002529094A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10788398P 1998-11-09 1998-11-09
US60/107,883 1998-11-09
PCT/EP1999/008506 WO2000028052A2 (en) 1998-11-09 1999-11-05 Nucleic acid molecules from rice encoding an r1 protein and their use for the production of modified starch

Related Child Applications (1)

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JP2011017984A Division JP5494980B2 (ja) 1998-11-09 2011-01-31 コメ由来核酸分子および改変デンプンの生産のためのその使用

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JP2002529094A JP2002529094A (ja) 2002-09-10
JP2002529094A5 true JP2002529094A5 (ja) 2011-04-07

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JP2000581219A Withdrawn JP2002529094A (ja) 1998-11-09 1999-11-05 コメ由来核酸分子および改変デンプンの生産のためのその使用
JP2011017984A Expired - Lifetime JP5494980B2 (ja) 1998-11-09 2011-01-31 コメ由来核酸分子および改変デンプンの生産のためのその使用

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US (2) US6521816B1 (ja)
EP (1) EP1131452B1 (ja)
JP (2) JP2002529094A (ja)
CN (1) CN100408687C (ja)
AR (1) AR021130A1 (ja)
AU (1) AU773808B2 (ja)
BR (1) BR9915152A (ja)
CA (1) CA2348366C (ja)
WO (1) WO2000028052A2 (ja)

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