IL126100A - Recombinant thermostable dna polymerase having reduced level of discrimination, nucleic acid sequence encoding it and method for its preparation - Google Patents

Recombinant thermostable dna polymerase having reduced level of discrimination, nucleic acid sequence encoding it and method for its preparation

Info

Publication number
IL126100A
IL126100A IL12610098A IL12610098A IL126100A IL 126100 A IL126100 A IL 126100A IL 12610098 A IL12610098 A IL 12610098A IL 12610098 A IL12610098 A IL 12610098A IL 126100 A IL126100 A IL 126100A
Authority
IL
Israel
Prior art keywords
thermostable dna
dna
recombinant
present
polymerases
Prior art date
Application number
IL12610098A
Other languages
English (en)
Other versions
IL126100A0 (en
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of IL126100A0 publication Critical patent/IL126100A0/xx
Publication of IL126100A publication Critical patent/IL126100A/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IL12610098A 1997-09-11 1998-09-07 Recombinant thermostable dna polymerase having reduced level of discrimination, nucleic acid sequence encoding it and method for its preparation IL126100A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5852597P 1997-09-11 1997-09-11

Publications (2)

Publication Number Publication Date
IL126100A0 IL126100A0 (en) 1999-05-09
IL126100A true IL126100A (en) 2005-12-18

Family

ID=22017355

Family Applications (1)

Application Number Title Priority Date Filing Date
IL12610098A IL126100A (en) 1997-09-11 1998-09-07 Recombinant thermostable dna polymerase having reduced level of discrimination, nucleic acid sequence encoding it and method for its preparation

Country Status (18)

Country Link
US (3) US6346379B1 (de)
EP (1) EP0902035B1 (de)
JP (1) JP4417452B2 (de)
KR (1) KR100561122B1 (de)
CN (1) CN1197962C (de)
AT (1) ATE530567T1 (de)
AU (1) AU741366B2 (de)
BR (1) BR9803419B1 (de)
CA (1) CA2243985C (de)
CZ (1) CZ293217B6 (de)
ES (1) ES2374910T3 (de)
HK (1) HK1018796A1 (de)
HU (1) HUP9802024A3 (de)
IL (1) IL126100A (de)
NO (1) NO327476B1 (de)
PL (1) PL328525A1 (de)
RU (1) RU2238978C2 (de)
TW (1) TW513439B (de)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2264045B1 (de) 1996-08-14 2015-10-21 Life Technologies Corporation Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20030138834A1 (en) * 1998-08-19 2003-07-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US20040121394A1 (en) * 1998-08-19 2004-06-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
JP2003507072A (ja) * 1999-08-21 2003-02-25 アマシャム・バイオサイエンス・コーポレイション 耐塩性を改善した、E681にアミノ酸置換を有するTaqDNAポリメラーゼおよびその同族体
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
EP2801624B1 (de) * 2001-03-16 2019-03-06 Singular Bio, Inc Arrays und Verfahren zur Verwendung
AU2002316586A1 (en) * 2001-07-06 2003-01-21 Amersham Biosciences Corp Novel dna polymerases having amino acid substitutions and homologs thereof
JP2005517413A (ja) * 2002-02-12 2005-06-16 アプレラ コーポレイション ポリメラーゼ組成物
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
KR100571808B1 (ko) * 2003-04-16 2006-04-17 삼성전자주식회사 다층 박막 구조를 가진 dna 칩
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005054441A2 (en) * 2003-12-01 2005-06-16 California Institute Of Technology Device for immobilizing chemical and biomedical species and methods of using same
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
ATE507305T1 (de) * 2004-05-25 2011-05-15 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7524831B2 (en) * 2005-03-02 2009-04-28 Schering Corporation Treatments for Flaviviridae virus infection
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
GB0518867D0 (en) * 2005-09-15 2005-10-26 Secretary Trade Ind Brit Microscopy tip
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
CA2633476C (en) * 2005-12-22 2015-04-21 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
CN101365807A (zh) 2005-12-22 2009-02-11 加利福尼亚太平洋生物科学股份有限公司 用于掺入核苷酸类似物的聚合酶
EP1963501A4 (de) * 2005-12-22 2009-04-22 Pacific Biosciences California Proteinherstellungsstrategien zur optimierung der aktivität von proteinen mit oberflächenhaftung
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2089517A4 (de) * 2006-10-23 2010-10-20 Pacific Biosciences California Polymeraseenzyme und reagenzien für erweiterte nukeinsäuresequenzierung
US20100081927A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081916A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware. Histological facilitation systems and methods
US20100081928A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological Facilitation systems and methods
US20100081915A1 (en) * 2008-09-29 2010-04-01 Searete Llc, Alimited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081924A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081926A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
FR2940291B1 (fr) 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2940125B1 (fr) 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
EP2582801B1 (de) 2010-06-18 2015-04-22 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
WO2011157435A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CA2802302C (en) 2010-06-18 2016-04-26 F. Hoffman-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103025870B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
AU2011267421B2 (en) 2010-06-18 2014-04-24 F. Hoffmann-La Roche Ag DNA polymerases with increased 3'-mismatch discrimination
EP2675897B1 (de) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz
JP6023173B2 (ja) 2011-04-11 2016-11-09 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改良された活性を有するdnaポリメラーゼ
ES2553400T3 (es) 2011-07-28 2015-12-09 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
ES2668448T3 (es) 2011-12-08 2018-05-18 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
JP6140182B2 (ja) 2011-12-08 2017-05-31 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改善された活性を有するdnaポリメラーゼ
EP2788480B1 (de) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
WO2015026873A1 (en) 2013-08-19 2015-02-26 Singular Bio, Inc. Assays for single molecule detection and use thereof
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
EP3712261A1 (de) 2015-02-02 2020-09-23 F. Hoffmann-La Roche AG Polymerasevarianten und verwendungen davon
CN113528623A (zh) 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
JP6963238B2 (ja) 2015-11-27 2021-11-05 国立大学法人九州大学 Dnaポリメラーゼ変異体
WO2020053327A1 (en) 2018-09-13 2020-03-19 F. Hoffmann-La Roche Ag Mutant dna polymerase(s) with improved strand displacement ability
CN117083392A (zh) * 2021-03-19 2023-11-17 深圳华大智造科技股份有限公司 用于有效掺入核苷酸与3’-磷酸酯和其他3’-终止子的聚合酶

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
CA2018273C (en) * 1989-06-09 1999-04-06 Peter D. Senter Thermally stable cytosine deaminase
US5338678A (en) * 1989-06-09 1994-08-16 Oncogen, A Limited Partnership Expression of DNA sequences encoding a thermally stable cytosine deaminase from saccharomyces
US5292658A (en) * 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5446591A (en) * 1993-02-08 1995-08-29 Lockheed Missiles & Space Co., Inc. Lens mounting for use with liquid lens elements
US5571706A (en) * 1994-06-17 1996-11-05 The United States Of America As Represented By The Secretary Of Agriculture Plant virus resistance gene and methods
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DE655506T1 (de) * 1994-10-17 1995-09-28 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.
US5852191A (en) * 1995-06-07 1998-12-22 Carnegie Mellon University Rigidized monomethine cyanines
US6107029A (en) * 1996-07-31 2000-08-22 Message Pharmaceticals, Inc. Universal method for detecting interactions between RNA molecules and RNA binding proteins
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
EP0983364B1 (de) 1997-03-12 2002-06-12 PE Corporation (NY) Dna polymerasen, die verbesserte fähigkeiten zum einbau markierter nukleotide besitzen

Also Published As

Publication number Publication date
AU741366B2 (en) 2001-11-29
HK1018796A1 (en) 2000-01-07
HUP9802024A2 (hu) 1999-05-28
RU2238978C2 (ru) 2004-10-27
KR100561122B1 (ko) 2006-07-25
IL126100A0 (en) 1999-05-09
ES2374910T3 (es) 2012-02-23
TW513439B (en) 2002-12-11
JPH11137284A (ja) 1999-05-25
EP0902035A3 (de) 1999-06-23
EP0902035B1 (de) 2011-10-26
US20030152988A1 (en) 2003-08-14
NO984157L (no) 1999-03-12
HU9802024D0 (en) 1998-12-28
EP0902035A2 (de) 1999-03-17
CZ293217B6 (cs) 2004-03-17
AU8416198A (en) 1999-03-25
ATE530567T1 (de) 2011-11-15
CN1197962C (zh) 2005-04-20
CA2243985C (en) 2004-08-10
US20020142333A1 (en) 2002-10-03
US6346379B1 (en) 2002-02-12
CA2243985A1 (en) 1999-03-11
CN1218832A (zh) 1999-06-09
KR19990029676A (ko) 1999-04-26
NO327476B1 (no) 2009-07-13
HUP9802024A3 (en) 2001-02-28
US7598032B2 (en) 2009-10-06
JP4417452B2 (ja) 2010-02-17
BR9803419A (pt) 2000-02-08
CZ287498A3 (cs) 1999-03-17
NO984157D0 (no) 1998-09-10
BR9803419B1 (pt) 2010-09-21
PL328525A1 (en) 1999-03-15

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