WO2002057479A3 - Primers and methods for the detection and discrimination of nucleic acids - Google Patents

Primers and methods for the detection and discrimination of nucleic acids Download PDF

Info

Publication number
WO2002057479A3
WO2002057479A3 PCT/US2001/050460 US0150460W WO02057479A3 WO 2002057479 A3 WO2002057479 A3 WO 2002057479A3 US 0150460 W US0150460 W US 0150460W WO 02057479 A3 WO02057479 A3 WO 02057479A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
primers
detection
discrimination
nucleic acids
Prior art date
Application number
PCT/US2001/050460
Other languages
French (fr)
Other versions
WO2002057479A2 (en
Inventor
Irina Nazarenko
Ayoub Rashtchian
Joseph Solus
Richard M Pires
Marlene Darfler
Gulilat Gebeyehu
Mekbib Astatke
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to NZ527128A priority Critical patent/NZ527128A/en
Priority to CA002433330A priority patent/CA2433330A1/en
Priority to JP2002558531A priority patent/JP2004532615A/en
Priority to EP01997138A priority patent/EP1353936A4/en
Publication of WO2002057479A2 publication Critical patent/WO2002057479A2/en
Publication of WO2002057479A3 publication Critical patent/WO2002057479A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in wide variety of molecular biology applications and are particularly useful in allele specific PCR.
PCT/US2001/050460 2000-12-27 2001-12-27 Primers and methods for the detection and discrimination of nucleic acids WO2002057479A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
NZ527128A NZ527128A (en) 2000-12-27 2001-12-27 Primers and methods for the detection and discrimination of nucleic acids
CA002433330A CA2433330A1 (en) 2000-12-27 2001-12-27 Primers and methods for the detection and discrimination of nucleic acids
JP2002558531A JP2004532615A (en) 2000-12-27 2001-12-27 Primers and methods for nucleic acid detection and identification
EP01997138A EP1353936A4 (en) 2000-12-27 2001-12-27 Primers and methods for the detection and discrimination of nucleic acids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US74814600A 2000-12-27 2000-12-27
US09/748,146 2000-12-27
US33046801P 2001-10-23 2001-10-23
US60/330,468 2001-10-23

Publications (2)

Publication Number Publication Date
WO2002057479A2 WO2002057479A2 (en) 2002-07-25
WO2002057479A3 true WO2002057479A3 (en) 2003-08-14

Family

ID=26987295

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050460 WO2002057479A2 (en) 2000-12-27 2001-12-27 Primers and methods for the detection and discrimination of nucleic acids

Country Status (5)

Country Link
EP (1) EP1353936A4 (en)
JP (1) JP2004532615A (en)
CA (1) CA2433330A1 (en)
NZ (1) NZ527128A (en)
WO (1) WO2002057479A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7081339B2 (en) * 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
EP1537240A2 (en) * 2002-09-06 2005-06-08 Johns Hopkins Singapore Pte Ltd Strand specific detection and quantification
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
EP1735459B1 (en) * 2004-04-07 2012-01-25 Exiqon A/S Methods for quantification of micrornas and small interfering rnas
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
CN1280422C (en) 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 Asymmetrical PCR amplification method, dedicated primer and use thereof
KR20070090158A (en) * 2004-10-18 2007-09-05 브랜데이스 유니버시티 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
ATE480644T1 (en) * 2005-01-25 2010-09-15 Rosetta Inpharmatics Llc METHOD FOR QUANTIFYING SMALL RNA MOLECULES
US8071306B2 (en) 2005-01-25 2011-12-06 Merck Sharp & Dohme Corp. Methods for quantitating small RNA molecules
EP2066813A2 (en) 2006-09-28 2009-06-10 Ensemble Discovery Corporation Compositions and methods for biodetection by nucleic acid-templated chemistry
GB0806676D0 (en) 2008-04-12 2008-05-14 Environment Agency The Environmental monitoring
EP2133434A1 (en) * 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Method for the detection of target nucleic acid
FR2940805B1 (en) * 2009-01-05 2015-10-16 Biomerieux Sa METHOD FOR AMPLIFYING AND / OR DETECTING NUCLEIC ACIDS, KITS AND USES THEREOF
WO2010147848A2 (en) * 2009-06-15 2010-12-23 Rd Biosciences, Inc. Kits and methods for selective amplification and detection of nucleic acid targets
GB0915664D0 (en) 2009-09-08 2009-10-07 Enigma Diagnostics Ltd Reaction method
SG185776A1 (en) 2010-06-14 2013-01-30 Univ Singapore Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr
JP6316185B2 (en) * 2011-05-06 2018-04-25 キアゲン ゲーエムベーハー Methods for sequencing, amplification and detection of nucleic acids containing internally labeled primers
UA125112C2 (en) 2012-04-09 2022-01-12 Енвіролоджикс Інк. METHOD OF AMPLIFICATION OF POLYNUCLEOTIDE
WO2015077427A1 (en) 2013-11-20 2015-05-28 The University Of Akron Highly Fluorescent Pyrrole-BF2 Chromophores
US9273078B2 (en) 2013-12-05 2016-03-01 The University Of Akron Half-phthalocyanine-like chelates and synthesis thereof
US11505836B2 (en) 2014-04-22 2022-11-22 Envirologix Inc. Compositions and methods for enhancing and/or predicting DNA amplification
CN114507715A (en) * 2014-11-20 2022-05-17 安普里怀斯公司 Compositions and methods for nucleic acid amplification
WO2023129167A1 (en) * 2021-12-30 2023-07-06 Cue Health Inc. Compositions and methods for isothermal amplification of nucleic acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6248526B1 (en) * 1997-12-15 2001-06-19 Aventis Behring, Gmbh Labeled primer for use in and detection of target nucleic acids

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6379888B1 (en) * 1999-09-27 2002-04-30 Becton, Dickinson And Company Universal probes and methods for detection of nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6248526B1 (en) * 1997-12-15 2001-06-19 Aventis Behring, Gmbh Labeled primer for use in and detection of target nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1353936A4 *

Also Published As

Publication number Publication date
EP1353936A4 (en) 2005-05-18
JP2004532615A (en) 2004-10-28
NZ527128A (en) 2005-11-25
WO2002057479A2 (en) 2002-07-25
EP1353936A2 (en) 2003-10-22
CA2433330A1 (en) 2002-07-25

Similar Documents

Publication Publication Date Title
WO2000079009A3 (en) Improved primers and methods for the detection and discrimination of nucleic acids
WO2002057479A3 (en) Primers and methods for the detection and discrimination of nucleic acids
WO2001071042A3 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
AU2002365811A1 (en) Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations
AU3064200A (en) A method of dna sequencing
WO2002002806A3 (en) Method and nucleic acids for analysing the methylation of genes implicated in pharmacogenomics
WO2002000934A3 (en) Multiply-primed amplification of nucleic acid sequences
MX9705961A (en) THERMOSTABLE DNA POLYMERASE HAVING REDUCED DISCRIMINATION AGAINST RIBO-NTPs.
WO2005044836A3 (en) Macromolecular nucleotide compounds and methods for using the same
EP1591541A3 (en) Method of nucleic acid sequencing
EP1431303A3 (en) Composition for linear isothermal amplification of polynucleotide sequences
EP2500440A3 (en) Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof
AU2002321094A1 (en) A novel group of alpha-amylases and a method for identification and production of novel alpha-amylases
CA2172722A1 (en) Methods and compositions for efficient nucleic acid sequencing
WO2002068579A3 (en) Kits, such as nucleic acid arrays, comprising a majority of human exons or transcripts, for detecting expression and other uses thereof
WO2001013086A3 (en) Detection of nucleic acids
WO2001096612A3 (en) Method of identifying and quantifying specific fungi and bacteria
AU2002365912A1 (en) Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
WO2002061133A3 (en) Nucleic acid detection using structured probes
AU7104300A (en) Method for amplifying signal-flanking sequences from unknown genomic dna
WO2003040397A3 (en) Asymmetric pcr with nuclease-free polymerase or nuclease-resistant molecular beacons
WO2000066771A3 (en) Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
WO2001057058A3 (en) Detection of differential gene expression
AU1728001A (en) Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample
WO2003052099A3 (en) Methods of parallel gene cloning and analysis

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2433330

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2002558531

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 527128

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 2002248257

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001997138

Country of ref document: EP

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWP Wipo information: published in national office

Ref document number: 2001997138

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 527128

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 527128

Country of ref document: NZ

WWW Wipo information: withdrawn in national office

Ref document number: 2001997138

Country of ref document: EP