MX371428B - Ensayos para la deteccion de una sola molecula y uso de los mismos. - Google Patents

Ensayos para la deteccion de una sola molecula y uso de los mismos.

Info

Publication number
MX371428B
MX371428B MX2016002218A MX2016002218A MX371428B MX 371428 B MX371428 B MX 371428B MX 2016002218 A MX2016002218 A MX 2016002218A MX 2016002218 A MX2016002218 A MX 2016002218A MX 371428 B MX371428 B MX 371428B
Authority
MX
Mexico
Prior art keywords
probes
marker
probe
tracer
immobilized
Prior art date
Application number
MX2016002218A
Other languages
English (en)
Other versions
MX2016002218A (es
Inventor
Nielsen Fehr Adrian
James Collins Patrick
Lyndon Herschleb Jill
Bowden JONES Hywel
Original Assignee
Singular Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Singular Bio Inc filed Critical Singular Bio Inc
Publication of MX2016002218A publication Critical patent/MX2016002218A/es
Publication of MX371428B publication Critical patent/MX371428B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a un método para detectar una variación genética en una muestra genética proveniente de un sujeto, caracterizado porque comprende: poner en contacto un primero y un segundo conjunto de sondas con la muestra genética, donde el primer conjunto de sondas comprende una primera sonda marcadora y una primera sonda trazadora, y el segundo conjunto de sondas comprende una segunda sonda marcador ay una segunda sonda trazadora, hibridar por lo menos algunas partes del primero y el segundo conjunto de sondas con unas primera y segunda regiones de ácido nucleico de interés en las moléculas de nucleótidos de la muestra genética, respectivamente, ligar el primer conjunto de sondas, ligando por lo menos la primer sonda marcadora y la primera sonda trazadora, ligar el segundo conjunto de sondas, ligando por lo menos la segunda sonda marcadora y la segunda sonda marcadora y la segunda sonda trazadora, opcionalmente, amplificar los conjuntos de sondas ligadas, inmovilizar las sondas trazadoras en una posición predeterminada sobre un sustrato, donde las primera y las segundas sondas marcadoras y/o las sondas marcadoras amplificadas de las mismas ligadas a las sondas trazadoras inmovilizada comprende primeros y segundos marcadores, respectivamente, los primero y segundos marcadores son diferentes, los marcadores inmovilizados e pueden resolver ópticamente por tipo de marcador, las primeras y segundas sondas trazadoras inmovilizadas y/o las sondas trazadoras amplificadas de las mismas comprende primeros y segundos trazadores, respectivamente, y llevar a cabo el paso de inmovilización inmovilizando a los trazadores en la posición predeterminada, contar (i) un primer número del primer marcador inmovilizado en el sustrato, y (ii) un segundo número del segundo marcador inmovilizado en el sustrato, y comparar los primeros y segundos números para determinar la variación genética en la muestra genética.
MX2016002218A 2013-08-19 2014-08-19 Ensayos para la deteccion de una sola molecula y uso de los mismos. MX371428B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361867554P 2013-08-19 2013-08-19
US201361867559P 2013-08-19 2013-08-19
PCT/US2014/051763 WO2015026873A1 (en) 2013-08-19 2014-08-19 Assays for single molecule detection and use thereof

Publications (2)

Publication Number Publication Date
MX2016002218A MX2016002218A (es) 2016-11-18
MX371428B true MX371428B (es) 2020-01-30

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MX2016002218A MX371428B (es) 2013-08-19 2014-08-19 Ensayos para la deteccion de una sola molecula y uso de los mismos.
MX2019015016A MX2019015016A (es) 2013-08-19 2016-02-19 Ensayos para la deteccion de una sola molecula y uso de los mismos.

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Country Status (13)

Country Link
US (5) US9212394B2 (es)
EP (3) EP3726213A1 (es)
JP (2) JP6088710B2 (es)
CN (2) CN110283888B (es)
AU (2) AU2014308980C1 (es)
BR (1) BR112016003480B1 (es)
CA (1) CA2921628A1 (es)
ES (2) ES3057032T3 (es)
IL (3) IL285521B2 (es)
MX (2) MX371428B (es)
SG (1) SG11201601201VA (es)
WO (1) WO2015026873A1 (es)
ZA (1) ZA201601029B (es)

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