WO1998045452A3 - Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof - Google Patents

Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof Download PDF

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Publication number
WO1998045452A3
WO1998045452A3 PCT/US1998/006921 US9806921W WO9845452A3 WO 1998045452 A3 WO1998045452 A3 WO 1998045452A3 US 9806921 W US9806921 W US 9806921W WO 9845452 A3 WO9845452 A3 WO 9845452A3
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WO
WIPO (PCT)
Prior art keywords
preparation
functions
enzyme derived
thermophilic organisms
replicase
Prior art date
Application number
PCT/US1998/006921
Other languages
French (fr)
Other versions
WO1998045452A2 (en
Inventor
Olga Yurieva
John Kuriyan
Michael E O'donnell
David Jeruzalmi
Original Assignee
Univ Rockefeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Rockefeller filed Critical Univ Rockefeller
Priority to EP98924742A priority Critical patent/EP0983365A1/en
Priority to AU76839/98A priority patent/AU7683998A/en
Publication of WO1998045452A2 publication Critical patent/WO1998045452A2/en
Publication of WO1998045452A3 publication Critical patent/WO1998045452A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in Thermus thermophilus, and corresponds to Polymerase III in E. coli. The genes and the polypeptides corresponding to T.th.. η, τ, ε, α and β subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.
PCT/US1998/006921 1997-04-08 1998-04-08 Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof WO1998045452A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98924742A EP0983365A1 (en) 1997-04-08 1998-04-08 Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
AU76839/98A AU7683998A (en) 1997-04-08 1998-04-08 Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82340797A 1997-04-08 1997-04-08
US08/823,407 1997-04-08

Publications (2)

Publication Number Publication Date
WO1998045452A2 WO1998045452A2 (en) 1998-10-15
WO1998045452A3 true WO1998045452A3 (en) 1998-12-17

Family

ID=25238686

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/006921 WO1998045452A2 (en) 1997-04-08 1998-04-08 Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof

Country Status (3)

Country Link
EP (1) EP0983365A1 (en)
AU (1) AU7683998A (en)
WO (1) WO1998045452A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0997530B1 (en) * 1997-06-26 2006-08-23 Takara Bio Inc. Dna polymerase-related factors
EP1012248B1 (en) * 1997-09-12 2008-07-02 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
US6238905B1 (en) 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
JP2002522042A (en) * 1998-08-06 2002-07-23 ライオン バイオサイエンス アクチェンゲゼルシャフト Thermostable in vitro complex with polymerase activity
DE19937230A1 (en) * 1999-08-06 2001-02-08 Lion Bioscience Ag Chimeric proteins
EP1088891B1 (en) * 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro
CN1307107A (en) * 2000-01-28 2001-08-08 上海博道基因技术有限公司 Polypeptide-DNA polymerase III 18 and polynucleotide for coding said polypeptide
EP1268813A2 (en) * 2000-03-28 2003-01-02 McHenry, Charles S. Thermophilic polymerase iii holoenzyme
US6677146B1 (en) 2000-03-28 2004-01-13 Replidyne, Inc. Thermophilic polymerase III holoenzyme
DE60231901D1 (en) * 2001-11-14 2009-05-20 Toyo Boseki DNA SYNTHESIS ENGINES, FACTORS ASSOCIATED WITH DNA POLYMERASE, AND USE THEREOF
WO2005113810A2 (en) * 2004-04-07 2005-12-01 Eppendorf Ag One component and two component dna pol iii replicases and uses thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009950A1 (en) * 1989-12-22 1991-07-11 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
WO1993015115A1 (en) * 1992-01-24 1993-08-05 Cornell Research Foundation, Inc. E. coli dna polymerase iii holoenzyme and subunits
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
US5633159A (en) * 1995-03-10 1997-05-27 Becton, Dickinson And Company DNA polymerase III β-subunit from mycobacteriophage DS6A

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009950A1 (en) * 1989-12-22 1991-07-11 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
WO1993015115A1 (en) * 1992-01-24 1993-08-05 Cornell Research Foundation, Inc. E. coli dna polymerase iii holoenzyme and subunits
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
US5633159A (en) * 1995-03-10 1997-05-27 Becton, Dickinson And Company DNA polymerase III β-subunit from mycobacteriophage DS6A

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J.C. ALONSO ET AL.: "Molecular cloning, genetic characterization and DNA sequence analysis of the recM region of Bacillus subtilis", NUCLEIC ACIDS RESEARCH, vol. 18, no. 23, 1990, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 6771 - 6777, XP002075008 *
MCHENRY C S ET AL: "A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium.", JOURNAL OF MOLECULAR BIOLOGY, (1997 SEP 19) 272 (2) 178-89. JOURNAL CODE: J6V. ISSN: 0022-2836., XP002075007 *
YURIEVA O ET AL: "Thermus thermophilis dnaX homolog encoding gamma- and tau-like proteins of the chromosomal replicase.", JOURNAL OF BIOLOGICAL CHEMISTRY, (1997 OCT 24) 272 (43) 27131-9. JOURNAL CODE: HIV. ISSN: 0021-9258., XP002075006 *
Z. TSUCHIHASHI AND A. KORNBERG: "Translational frameshifting generates the gamma subunit of DNA polymerase III holoenzyme", PROC. NATL. ACAD. SCI., vol. 87, April 1990 (1990-04-01), NATL. ACAD. SCI.,WASHINGTON,DC,US;, pages 2516 - 2520, XP002075009 *

Also Published As

Publication number Publication date
EP0983365A1 (en) 2000-03-08
AU7683998A (en) 1998-10-30
WO1998045452A2 (en) 1998-10-15

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