WO2001073052A3 - Thermophilic polymerase iii holoenzyme - Google Patents

Thermophilic polymerase iii holoenzyme Download PDF

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Publication number
WO2001073052A3
WO2001073052A3 PCT/US2001/009950 US0109950W WO0173052A3 WO 2001073052 A3 WO2001073052 A3 WO 2001073052A3 US 0109950 W US0109950 W US 0109950W WO 0173052 A3 WO0173052 A3 WO 0173052A3
Authority
WO
WIPO (PCT)
Prior art keywords
polymerase iii
iii holoenzyme
dna polymerase
present
thermophilic polymerase
Prior art date
Application number
PCT/US2001/009950
Other languages
French (fr)
Other versions
WO2001073052A2 (en
Inventor
Charles S Mchenry
Nebojsa Janjic
John Randall Bullard
Vladimir Kery
Original Assignee
Replidyne Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Replidyne Inc filed Critical Replidyne Inc
Priority to AU5106001A priority Critical patent/AU5106001A/en
Priority to EP01924402A priority patent/EP1268813A2/en
Priority to CA002404417A priority patent/CA2404417A1/en
Priority to AU2001251060A priority patent/AU2001251060B8/en
Priority to JP2001570769A priority patent/JP2003528612A/en
Publication of WO2001073052A2 publication Critical patent/WO2001073052A2/en
Publication of WO2001073052A3 publication Critical patent/WO2001073052A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to gene and amino acid sequences encoding DNA polymerase III holoenzyme subunits and structural genes from thermophilic organisms. In particular, the present invention provides DNA polymerase III holoenzyme subunits and accessory proteins of T. thermophilus. The present invention also provides antibodies, primers, probes, and other reagents useful to identify DNA polymerase III molecules.
PCT/US2001/009950 2000-03-28 2001-03-28 Thermophilic polymerase iii holoenzyme WO2001073052A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU5106001A AU5106001A (en) 2000-03-28 2001-03-28 Novel thermophilic polymerase iii holoenzyme
EP01924402A EP1268813A2 (en) 2000-03-28 2001-03-28 Thermophilic polymerase iii holoenzyme
CA002404417A CA2404417A1 (en) 2000-03-28 2001-03-28 Thermophilic polymerase iii holoenzyme
AU2001251060A AU2001251060B8 (en) 2000-03-28 2001-03-28 Thermophilic polymerase III holoenzyme
JP2001570769A JP2003528612A (en) 2000-03-28 2001-03-28 Novel thermophilic polymerase III holoenzyme

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19273600P 2000-03-28 2000-03-28
US60/192,736 2000-03-28

Publications (2)

Publication Number Publication Date
WO2001073052A2 WO2001073052A2 (en) 2001-10-04
WO2001073052A3 true WO2001073052A3 (en) 2002-10-24

Family

ID=22710851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/009950 WO2001073052A2 (en) 2000-03-28 2001-03-28 Thermophilic polymerase iii holoenzyme

Country Status (5)

Country Link
EP (1) EP1268813A2 (en)
JP (1) JP2003528612A (en)
AU (1) AU5106001A (en)
CA (1) CA2404417A1 (en)
WO (1) WO2001073052A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7556958B1 (en) 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
AU2001278935B2 (en) * 2000-07-14 2007-03-22 Replidyne, Inc. Novel DNA polymerase iii holoenzyme delta subunit nucleic acid molecules and proteins
KR102254209B1 (en) * 2021-01-15 2021-05-18 씨제이제일제당 (주) Novel DNA polymerase Ⅲ subunits gamma and tau variant and a method for producing L-lysine using the same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO1999013060A1 (en) * 1997-09-12 1999-03-18 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
WO1999053074A1 (en) * 1998-04-09 1999-10-21 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO1999013060A1 (en) * 1997-09-12 1999-03-18 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
WO1999053074A1 (en) * 1998-04-09 1999-10-21 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
C. MCHENRY ET AL.: "A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium", J. MOL. BIOL., vol. 272, 1997, pages 178 - 189, XP002075007 *
HIRAMATSU ET AL.: "Cloning and characterisation of the uvrD gene from an extremely thermophilic bacterium, Thermus thermophilus HB8", GENE, vol. 199, 1997, pages 77 - 82, XP002207220 *
O. YURIEVA ET AL.: "T. thermophilus dnaX homolog encoding gamma and tau-like proteins of the chromosomal replicase", J. BIOL. CHEM., vol. 272, no. 43, 1997, pages 27131 - 27139, XP002075006 *

Also Published As

Publication number Publication date
WO2001073052A2 (en) 2001-10-04
JP2003528612A (en) 2003-09-30
AU5106001A (en) 2001-10-08
CA2404417A1 (en) 2001-10-04
EP1268813A2 (en) 2003-01-02
AU2001251060B2 (en) 2007-01-25

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