HUE028375T2 - TC 1507-es kukorica és módszer ennek detektálására - Google Patents

TC 1507-es kukorica és módszer ennek detektálására Download PDF

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HUE028375T2
HUE028375T2 HUE04751097A HUE04751097A HUE028375T2 HU E028375 T2 HUE028375 T2 HU E028375T2 HU E04751097 A HUE04751097 A HU E04751097A HU E04751097 A HUE04751097 A HU E04751097A HU E028375 T2 HUE028375 T2 HU E028375T2
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dna
sequence
seq
primer
sequences
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Eric Barbour
James W Bing
Guy A Cardineau
Robert F Cressman Jr
Manju Gupta
Locke Mary E Hartnett
David Hondred
Joseph W Keaschall
Michael G Koziel
Terry E Meyer
Daniel Moellenbeck
Kenneth E Narva
Wilas Nirunsuksiri
Steven W Ritchie
Marjorie L Rudert
Craig D Sanders
Aihua Shao
Steven J Stelman
David S Stucker
Laura A Tagliani
Zante William M Van
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Dow Agrosciences Llc
Du Pont
Pioneer Hi Bred Int
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Application filed by Dow Agrosciences Llc, Du Pont, Pioneer Hi Bred Int filed Critical Dow Agrosciences Llc
Publication of HUE028375T2 publication Critical patent/HUE028375T2/hu

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Claims (16)

  1. TC ISSUES KUKORICA ÉS MÓDSZER ENNEK DETEKTÁLÁSÁRA SZABADALMI I^ÉMYPONTOK
    1. Egy Izsfáif DNS: MÄä, msïîy tartalmaz egy »kíeoád szekvenciát s kővetkező szefeysfiei^azsnesitc számokkal azonosítva: 21,22.24,2δ vagy 27. % Egy iészist a TC1 5G?-es .feâÂé: azonösításár a egy biológiai mintában, mely detektálta: a IC 150? spéeikos régiót.; ás mely steifte® készlet terteimaz egy első gÉHí; amely Mtsmsn a kővetkező szekvencía-azormsító számoM; 21 vagy 22, éa agy második prímért amely {elismerj a szekvendo-azonositó szám: 25-öt.
  2. 3. A késztet a 2-es igénypont: szepni aboi legalább az emiMt első és mása#: primer rendre tertaSmazzá az 1-es és a 2-es szekvencsâ-azcnosSo számú szekvenciát
  3. 4. A MmM b 2-es igénypont; s zebnt, sítől; legalább az említet első és második príméi màÊ fáítsimazza á: tm: és; a :23«8S szakváircia^zöaasitp számú szekvenciát.
  4. 5. A készlet a 2-es igénypte® ézenni Haj legalább az említett első és második primer rendre tartalmazza a ;3~as és; ;s 5-ős szekvencp-azoaosiíó: számú szekvenciái δ A késztet a 2-es igénypont szerint abc! legalább az említett első és második primer rendre tartalmazzam 4-es és a 5-ös szekvencia-azonosító számú szekvenciát. t Egy SMS defekciós késhet, mély taííate legeibb egy SIS molekula?, amely bomolág vagy iksmplementer a szekvencia-azonosító szám: 26-taí vagy 22-lei, ahol a DNS molekuía specifikus a TC1507 kukorica DNS csatlakozásra és egy elegendően bosszú egybefüggő DNS poiínukteoircL Hogy a DNS detekdos módszerben használható legyen.
  5. 8. Egy késztet a TC1 50?-es kukorica azonosítására egy biológiai mintában, mely készlet tsrtsimaz: egy specifikus szondát, mely tartalmaz egy szekvenciát, ami hibódizá! a szekvsncís-azonosító szám: 21-gyei és szekvencia-azonosító szám: MM, szókkal egybefüggően. 9 Egy készlet sfCISST-eskakofica azonosítására agy bioíógiai mintában, mely készlet tartalmaz: egy specifikus szondát, mely tartalmaz egy szekvenciát, ami hMzáí a szekvencia-azonosító szám:; 22-vei és szekvencia-azonosító szám: 2SNe|: azokkal egybefüggően, 1Ö. Egy SIS detekd^ készief, mely tadalmazegy prinuktesfid szondái amely bfbridizái szigorú hibridizációs ktirnények között agy vagy több DNS szekvenciává a szekssnsis^Bsnoslö szám: 45. 46,47, B, 61, 52, 54. 55, M m Sí, és szók kompíemeráeránek csopoi^ábdi kiválasztva.
  6. 11. Egy DNA detekcíss késiét, mely tartalmaz egy pnmor párt, melynek mindegyike tártaimat legalább tíz nukieotfdo? a szgksëàôiâ^dnàsiiô 26 és szám: 2? kózíl, melyben; mfedegyik iegy fôlSP beágyazásra irárryaió szekvencia diagnosztika ellentétes oldalain taiáifrató.
  7. 12. Egy módszer a TC 195? kttkorica azonosítására egy biológiai minfefrsn, raeiy módszer tartalmazza egy TOíSÖ? speçifes sigié detektálásátegy szondával vagy első pirnerret, amely kifejezetten egy szekvenciát Ismer lel a azskvetóa-azéfiöSító szám: 2:1 vsgy szám: 22 kazúí. 13. k módszer é 12-es Igénypont szerint, mely fertaimszza egy pfit fragment amlifikáisai az említett biológia mintában leíeeiéyóeökletnsavbíi, egy, legalább léi srimenai történő pcimsráz tánereakoío haszrfeíatápf, ahoi az említett efeo grimer felismer egy szekvenciát a kővetkező szekvencia-azonosító számon betűi: 21 vagy 22, és egy másod! primer felismer egy szekvenciára követező szekvencia-azonosító számon bei!: 25. Ï4 A medszer a 13-as igénypont szerint, ám az említett eisé primer felismer egy szekvenciát a. következő szekvenciád szsnoslo Számon beiöi: 21,. és az eratietf rnäscÄgnmer felismer egy szekvenciái a következő szekvencia-azonosife számos beli: 25. |5,: Ä módszer s l&es igénypont szerint, ahol m emieifeiső primer felismer egy szekvenciái a következe szekvéosía-áZSOösífe számon béli: 22, és égy második primer felismer egy szekvenciáié következő szekveneia-azoposlo számon beik 25.
  8. 16. A módszer á 14-éS igéhypéot szerint, ahol az emiMf első es másaik primer rendre tartaímazzék ® szek^nia-azonosiió: szám: 2-t és szekvencia-azonosító szám: 1-t; vsgy rendre tartalmazzák a szekvencia-azonosító szám. 2-t és szekvencia-azonosító szám- 23-t. 1?. A módszer a 15- es Igénypont szerit, ahol az míM eiső és második primer rendre tartalmazzák a; szekveoia-azoeosito szám; M ás szekvancia-azonosáo Szám: δ-t; vagy rendre tartalmazzák a szekvenda-azsnesitó számi 4-t és székveoda-gzsnos;íofeZám:§-t,
  9. 18. A módszer a íá-os igénypont szeánt, maiy TC1Ô87 POR azonosító mféokdií ftasznäatava); íadaímazza egy fragment ampiifikáeiójat keribeii §12 bp-rg, korüibfeöl S44 bp-re, koröibeíöi 342 bp-re vagy körpbeíuí 252 bp-rn IS. Egy módszer a TG1 S§7-es kufesdcs jeiefeéfenek detektáláséra vágy annak ieszarmazottpra: egy bioiegfei mintában, tartalmazva: egy DNS minta ifeyerésé? az#tíifettiöídgaí mintából.: szekvencia-azonosító szám: 1 és szakvencia-azoncsité szám: 2, szekvencia-azonosító szám; 2 és szekvencia-azonosító szám: M, székvsoefe-azonosífö szám; 3 és szekvencia-azonosító szám: 5, vagy szekvenda-azenosp szám: 4 és szekyencls-azonositó szám: 5 DNS primer molekÄ igositäMt; DNS amplitláoiós rsaköó feltéteieinek biztosítását; az emiatt DNS ampiifikációs reakció vigíghajtásp. ezáítá egy DNSampkkon mofekia gyÉtSsáfeés az említett SMS amplikon moiekoís detektálási, ahol az: említett DNS ampitken mOiékoia óetekíáiása az említet DNS ampiíkkáclös folyamatban jelzi a TCí5<2?-es kukorica ieieníétét
  10. 25,Egy izolált DNS msfetela, mely íadaimazzaa tS-es Igénypert szedni r#dszeráltal gyártott: amglikonl 21, % ráÉá^-á TßW^sk megfefelö DNS ^δβ»Ι detektálására egy mintában* mely módszer tartalmazza; à kötete OtöS-i MM mM&amp; érintkezését egy pstet^ísoM: szondával, mely hibbdizsi szigoré hibridizációs fesiímények iitl a f£1507 kukoricából származó DNS-sel, és nem ttiáE szigorú hifeudizádös terettrinyek között ^ oem-ICIStf növény DNS-éveí; » ínintáés 3: szonda áiávstésébszlgcRl hibridizációs körufmóayetesk; és 8 szstidéte s DNS-sel vétó hibridizációjának detektálását, ahol a hibridizáció rfetektá'sss a TS1S0? kukorica Jeiea^éf;:mát8§a ki:
    22, Egy izoiit: DNS ncieottd primer szekvencia, mely tartalmaz egy szekvenciát a; szekvei&amp;ja*áZtessitő szám: 4$, 4%, 47,49, Sü, Sí, 52,S% S51:55 ésS^feassk kompiemenfemite tepörtjábOl kiválasztva. 23, ;Egy DNS móléKulapár,: mély tartalmaz: egy első DNS molekulát, melyben: $ DNS moiekylák a szskvenolateormsító szám: 2» vagy templemarrterének, szekvencia-azonosító szám;: 27 vagy komplementerének elegendően hosszan s-gybefüggö oufteldáí, hogy DNS primőrként vagy szondaként feakstsnáljate egy TÜ1SQ7 tektes növényből vagy annak Itezáitezotpbói kinyert DNS íőagnosztizaíáséhsz, 24 Egy izolál DNS IW, mély osatekozó -szefespÄ tartalmaz, mely tarlsteaz egy szekvertoíát @ szám: 45,46,47,49, 50.51, 52,54, 55, §6: és 57, és azok komplementereinek csoportjából kiválasztva.
    25. Egy módszer a mag: fejtiszíaságársak megmositéséhez, mely módszer tartalmazza egy 10450? spnikos régi detektálását egy specifikus primerrsi vagy szondával, amely egy mag mintában kifejtettén é|y szekvenciát ismer fél a szekvencia-azonosító szám: 21 vagy a szekveneia-azsnosítö szám; 22:közul.
  11. 26. Egy módszer a magok átvizsgálásához, TC75Q7 jelenit# keresve, mely módszer tartalmazza agy TC15Q7 spéciitek régió detektálásét egy sperrte főmerreí vagy szondával,; amely égy veíomagíéteíbó: kieszelten egy szekvenciát ismer fél 8 telveesfe-azoncsífe szám;: 21 vagy a szekvencía-szposító szám: ;22::tezöi:
  12. 27. Így izoiá! DiS stekteote, mely tartalmaz egy szstesneif olyan DNS csoperiií márnám, mely ÖNStek tornáznák legalább égy naklé steteenciä a szskvenda-azoooskó szám: 45, 45, 47, 49. :φ,. 51, 52. §4, 55, 55 és 57, és azok komplementereinekcsoportjábó; kiválasztva
  13. 29. Izolált DNS tekyepÄ egy pads, máyek minásgyié íarfatmáz legalább fiz nukleotidot és melyek együk egy egy TÖi 507 kötete diagnosziíá: ampiÏEmt előállító CBS amplífikásös járásban terülte Mtasználásra. 21 Az izolált DNS: SÄSiÄ párja- a 28tes igénypont szerint melyek mindegyike a szá»toié^ten&amp;stta szaræ 20: vagy s stevánbiateteastíó szám: 21 közút kérőit kiválasztásra.
  14. 30. Egy módszer a TÇ1587 beágyazás: jsf©n^Îèrîôk defeaikísíásérä :kyköficg: szövőn, mely tartalmaz:: egy primer pár kiválasztásét. mély mindegyike tartalmaz legalább tíz nukíeotíctp! a szekvencia-azormsíté szám: 2d vagy szek'vencis-azonesitó szám: 27 körűi, amelyben a pár minden -tággá az említett TC15C-7 beágyazásra irányuló szekvencia diagnosztika ellentétes oldatain taMihsío; az említett kukorica szövet mintájának érintkezésétm említett primer párral; DNS amptikáclo végrePáfását és egy ampákon äsÄisät ;3t Egy mádszer a-ilkáé igénypsË szerint, abets primerpr minden tagja a szekveneia-azonositó szám: 12, % 4;: 5, 1,¾ 8, % 10. 11. 12: 13, 14, IS, 11, 17, IS, 19, 2D, 23, 45, 46, 47, 49, S0, 51, 52, 54, 55, 55 és 57. és ezek komplementereinek cseporijahdi kérő kiválasztásra.
  15. 32. Egy módszere IC 1507 beágyazás jelsntéténsk detektáláséra kukenoaszövetben, mely tartalmazza: m émietr kökericaszövet egy mintájának inníkezését egy pofeukieöád szgnçâval, mely ibbáizi sigörú Mbnfzaciós köAénysk közit: egy vagy több: DNS szekvenciává! s szekvencía-azonosltó szám. 45,41,, 47,4S, 50,51, 52, 54, 55,56 és 57, és: szók komplementereit taíiaimazö csoportból kivaiaszvsv az: említett minta és a szonda aíaviésiísziprű ^itzéei^.lMiményekn^l· és δ szonda:Mzidizáckpra Irányuló analízist 33:. Egy ÖNS detekcios készlet a 11-es igénypont szedni, ebsl a: primer pár minden: tsgja: a szekvencia-azonosító szám: f, 2,3, 4, 5, 6, 7, % S, 10, 11. 12, 13, 14, 15. 16, 17, 16, 19, 20. 23. 45. 45. 47, 49, 50, 51. 52, 54, 55, 56 és 57, és ezek kom^émsbtereinsk csoportjából kéri kiválasztásra.
  16. 34, Egy módszer a: 7(3*50? azsnesiisárá egy bístépi miÄan, mely módszer detektálja á TG15D7 specifikus régiót a kővetkező szekvencia-azonosító számon belli 24,
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