BRPI0410027A - molécula e constructo de dna, kit e método para a identificação de dna e do evento tc1507 de milho, método para a confirmação de pureza e de screening de sementes e método para a produção de uma planta de milho resistente a inseto - Google Patents

molécula e constructo de dna, kit e método para a identificação de dna e do evento tc1507 de milho, método para a confirmação de pureza e de screening de sementes e método para a produção de uma planta de milho resistente a inseto

Info

Publication number
BRPI0410027A
BRPI0410027A BRPI0410027-1A BRPI0410027A BRPI0410027A BR PI0410027 A BRPI0410027 A BR PI0410027A BR PI0410027 A BRPI0410027 A BR PI0410027A BR PI0410027 A BRPI0410027 A BR PI0410027A
Authority
BR
Brazil
Prior art keywords
dna
event
kit
maize
screening
Prior art date
Application number
BRPI0410027-1A
Other languages
English (en)
Inventor
Eric Barbour
James W Bing
Guy A Cardineau
Robert F Cressman Jr
Manju Gupta
Mary E Hartnett Locke
David Hondred
Joseph W Keaschall
Michael G Koziel
Terry E Meyer
Daniel Moellenbeck
Kenneth E Narva
Wilas Nirunsuksiri
Steven W Ritchie
Marjorie L Rudert
Craig D Sanders
Aihua Shao
Steven J Stelman
David S Stucker
Laura A Tagliani
William M Van Zante
Original Assignee
Dow Agrosciences Llc
Pioneer Hi Bred Int
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Pioneer Hi Bred Int, Du Pont filed Critical Dow Agrosciences Llc
Publication of BRPI0410027A publication Critical patent/BRPI0410027A/pt
Publication of BRPI0410027B1 publication Critical patent/BRPI0410027B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Abstract

"MOLéCULA E CONSTRUCTO DE DNA, KIT E MéTODO PARA A IDENTIFICAçãO DE DNA E DO EVENTO TC1507 DE MILHO, MéTODO PARA A CONFIRMAçãO DE PUREZA E DE SCREENING DE SEMENTES E MéTODO PARA A PRODUçãO DE UMA PLANTA DE MILHO RESISTENTE A INSETO". A invenção provê composições de DNA que se referem a plantas transgênicas de milho resistentes a inseto. São também providos ensaios para detectar a presença do evento TC1507 baseado na seqüência de DNA do constructo recombinante inserido no genoma de milho e seqüências de DNA flanqueadoras do sítio de inserção. São providos kits e condições utilizáveis na condução dos ensaios.
BRPI0410027-1A 2003-05-02 2004-04-29 molécula de dna isolada, kit e método para identificação do evento tc1507, construto de dna, método para aumentar a resistência de uma planta a insetos, método de detecção da presença do evento tc1507, sequência de junção de dna isolado, método de screening de sementes, método para a produção de uma planta, método para a detecção da presença da inserção do evento tc1507, kit de detecção de dna BRPI0410027B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US46777203P 2003-05-02 2003-05-02
US60/467,772 2003-05-02
PCT/US2004/013538 WO2004099447A2 (en) 2003-05-02 2004-04-29 Corn event tc1507 and methods for detection thereof

Publications (2)

Publication Number Publication Date
BRPI0410027A true BRPI0410027A (pt) 2006-04-25
BRPI0410027B1 BRPI0410027B1 (pt) 2020-11-03

Family

ID=33435118

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0410027-1A BRPI0410027B1 (pt) 2003-05-02 2004-04-29 molécula de dna isolada, kit e método para identificação do evento tc1507, construto de dna, método para aumentar a resistência de uma planta a insetos, método de detecção da presença do evento tc1507, sequência de junção de dna isolado, método de screening de sementes, método para a produção de uma planta, método para a detecção da presença da inserção do evento tc1507, kit de detecção de dna

Country Status (19)

Country Link
US (9) US7288643B2 (pt)
EP (2) EP1620571B1 (pt)
JP (2) JP2006525028A (pt)
KR (1) KR101152465B1 (pt)
AR (1) AR044244A1 (pt)
AU (1) AU2004236718C1 (pt)
BR (1) BRPI0410027B1 (pt)
CA (1) CA2524493C (pt)
CL (2) CL2004000934A1 (pt)
DK (1) DK1620571T3 (pt)
ES (1) ES2546267T3 (pt)
HU (1) HUE028375T2 (pt)
MX (1) MXPA05011795A (pt)
NZ (1) NZ543450A (pt)
PL (1) PL1620571T3 (pt)
PT (1) PT1620571E (pt)
TW (1) TWI378103B (pt)
WO (1) WO2004099447A2 (pt)
ZA (1) ZA200508964B (pt)

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