CN113373174A - 玉米事件dp-004114-3及其检测方法 - Google Patents
玉米事件dp-004114-3及其检测方法 Download PDFInfo
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Abstract
本发明提供了涉及转基因抗昆虫性玉米植物的DNA组合物。也提供了基于插入玉米基因组中的重组构建体的DNA序列和在插入位点侧翼的DNA序列检测玉米事件DP‑004114‑3的存在的测定法。提供了在实施所述测定法中有用的试剂盒和条件。
Description
本申请是以下申请的分案申请:申请日:2010年12月16日;申请号:201610113418.2;发明名称:玉米事件DP-004114-3及其检测方法。
技术领域
本发明的实施方式涉及植物分子生物学领域,本发明的实施方式具体地涉及用于向植物赋予昆虫抗性的DNA构建体。本发明的实施方式更具体地涉及抗昆虫性玉米植物事件DP-004114-3并且涉及用于检测样品及其组合物中玉米事件DP-004114-3的存在的测定法。
背景技术
本发明的实施方式涉及抗昆虫性玉米(玉蜀黍(Zea mays))植物DP-004114-3,也称作“玉米系DP-004114-3”、“玉米事件DP-004114-3”和“4114玉米”,并且涉及玉米植物DP-004114-3的DNA植物表达构建体和在玉米植物DP-004114-3及其子代中检测转基因/侧翼插入区域。
玉米是一种重要作物并且是世界许多地区的主要食物来源。尽管采用了诸如化学杀虫剂之类的保护性措施,然而由害虫造成的损害仍是世界范围玉米作物损耗的主要因素。有鉴于此,已经将抗昆虫性基因工程化至作物如玉米中,以控制昆虫损害并降低对传统化学杀虫剂的需要。已经用于产生转基因抗昆虫性作物的一组基因是来自苏云金芽孢杆菌(Bacillus thuringiensis)(Bt)的δ-内毒素类。δ-内毒素已经成功地表达于农作植物如棉属植物、马铃薯、稻、向日葵以及玉米中,并且已经证明提供了优异的病害昆虫控制作用。(Perlak,F.J等人,(1990)Bio/Technology 8:939-943;Perlak,F.J.等人,(1993)PlantMol.Biol.22:313-321;Fujimoto,H.等人,(1993)Bio/Technology 11:1151-1155;Tu等人,(2000)Nature Biotechnology 18:1101-1104;PCT公开WO 01/13731;和Bing,J.W.等人,(2000)Efficacy of Cry1F Transgenic Maize,14thBiennial International PlantResistance to Insects Workshop,Fort Collins,CO)。
已知外来基因在植物中的表达受它们在植物基因组中的位置影响,这可能归因于染色质结构(例如,异染色质)或转录调节元件(例如,增强子)密切接近于整合位点(Weising等人,(1988)Ann.Rev.Genet.22:421-477)。与此同时,转基因在基因组中不同位置处的存在将以不同方式影响植物的总体表型。出于这个原因,经常需要筛选大量事件,以鉴定出以导入的相关基因的最佳表达为特征的事件。例如,已经在植物及在其他生物体中观察到,在事件之间可能存在导入基因的表达水平的巨大差异。在表达的空间或时刻模式方面也可以存在差异,例如,转基因在多种植物组织中相对表达方面的差异,这可能不对应于从存在于导入的基因构建体中的转录调节元件所预期的模式。出于这个原因,常见的是产生数百至数千个不同事件,并从这些事件中筛选出具有合乎需要的转基因表达水平和用于商业目的模式的单一事件。利用常规育种方法,具有合乎需要的转基因表达水平或模式的事件可用于将转基因通过有性远交渐掺至其他遗传背景中。此类杂交的子代维持原始转化体的转基因表达特征。使用这种策略以在充分适应于局部生长条件的众多品种中确保可靠的基因表达。
如能够检测特定事件的存在以确定有性杂交体的子代是否含相关的转基因,将是有利的。此外,用于检测特定事件的方法将有助于例如符合要求销售前批准和标注源自重组作物植物的食品的法规,或用于环境监测、在田间监测作物的性状、或监测源自作物收获物的产品、以及用于确保各方主体遵守法规条款或合同条款。
可以通过本领域已知的任何核酸检测方法,包括、但不限于聚合酶链反应(PCR)或使用核酸探针的DNA杂交法,来检测转基因的存在。这些检测方法通常集中于频繁使用的遗传元件,如启动子、终止子、标记基因等,因为对于许多DNA构建体而言,编码区是可互换的。因此,此类方法可能无法用于区分不同的事件,尤其是使用相同DNA构建体或十分相似的构建体所产生的那些事件,除非已知与插入的异源DNA相邻的侧翼DNA的DNA序列。例如,美国专利No 6,395,485中描述了一种用于检测原种事件GAT-ZM1的事件特异性PCR测定法。因此,期望拥有一种用于鉴定事件DP-004114-3的简单而又有甄别性的方法。
发明内容
本发明的实施方式涉及用于产生和选择抗昆虫性单子叶农作植物的方法。更具体地,提供了一种在植物细胞和植物中表达时赋予抗昆虫性的DNA构建体。根据本发明的一个方面,提供了一种能够导入宿主细胞中并且在其中复制的DNA构建体,所述DNA构建体在植物细胞和植物中表达时赋予所述植物细胞和植物抗昆虫性。玉米事件DP-004114-3由农杆菌介导的质粒PHP27118转化产生。该事件含有cry1F,cry34Ab1、cry35Ab1和pat基因盒,它们赋予针对某些鳞翅目(lepidopteran)和鞘翅目(coleopteran)害虫的抵抗性以及对膦丝菌素耐受性。
具体而言,第一盒含有截短形式的来自苏云金芽孢杆菌鲇泽变种(Bacillusthuringiensis var.aizawai)的cry1F基因。cry1F基因的插入赋予针对鳞翅目害虫损害的抗性。Cry1F蛋白(SEQ ID NO:1)由605个氨基酸组成并且具有大约68kDa的分子量。cry1F基因的表达受玉米多聚遍在蛋白启动子控制(Christensen等人,(1992)Plant Mol.Biol.118(4):675-89),从而提供Cry1F蛋白在玉米中的组成型表达。该区域还包括与天然多聚遍在蛋白启动子相连的5′非翻译区(UTR)和内含子。cry1F基因的终止子是来自根癌农杆菌(Agrobacterium tumefaciens)Ti质粒pTi15955的开放读框25(ORF 25)的聚(A)添加信号(Barker等人,(1983)PlantMol.Biol.2:335-350)。
第二盒含有从苏云金芽孢杆菌菌株PS149BS149B1分离的cry34Ab1基因(美国专利No.6,127,180;No.6,624,145和No.6,340,593)。Cry34Ab1蛋白(SEQ ID NO:2)的长度是123个氨基酸并且具有大约14kDa的分子量。cry34Ab1基因的表达受带有5′UTR和内含子的玉米多聚遍在蛋白启动子的第二副本控制(Christensen等人,1992,上文)。cry34Ab1基因的终止子是pinII终止子(Keil等人,(1986)Nucleic Acids Res.14:5641-5650;An等人,(1989)Plant Cell 1:115-22)。
第三基因盒含有cry35Ab1基因,其也从苏云金芽孢杆菌菌株PS149BS149B1分离而得(美国专利No.6,083,499;No.6,548,291和No.6,340,593)。Cry35Ab1蛋白(SEQ ID NO:3)具有383个氨基酸的长度和大约44kDa的分子量。Cry34Ab1和Cry35Ab1蛋白在植物中的同时表达赋予对鞘翅目昆虫的抗性。cry35Ab1基因的表达受普通小麦(Triticum aestivum)(小麦)过氧化物酶启动子和前导序列控制(Hertig等人,(1991)Plant Mol.Biol.16:171-174)。cry35Ab1基因的终止子是pinII终止子的第二副本(Keil等人,1986,上文;An等人,1989,上文)。
第四也是最后的基因盒含有来自绿色产色链霉菌(Streptomycesviridochromogenes)的膦丝菌素乙酰基转移酶基因(pat)的已经为玉米中表达进行优化的形式。pat基因表达赋予膦丝菌素耐受性的膦丝菌素乙酰基转移酶(PAT)。PAT蛋白(SEQ IDNO:4)的长度是183个氨基酸并且具有大约21kDa的分子量。pat基因的表达受来自CaMV 35S转录物的启动子区和终止子区控制(Franck等人,(1980)Cell 21:285-294;Odell等人,(1985)Nature 313:810-812;Pietrzak等人,(1986)Nucleic Acids Res.14(14):5857-5868)。还提供了含有所述DNA构建体的植物。
根据本发明的另一个实施方式,提供了用于鉴定命名为DP-004114-3的新玉米植物的组合物和方法。所述方法基于特异性识别DP-004114-3的5′和/或3′侧翼序列的引物或探针。提供了包含以下引物序列的DNA分子,其中在PCR反应中使用时,所述引物序列将产生对转基因事件DP-004114-3为独特的扩增子。包含这些分子的玉米植物和种子是本发明的一个实施方式。另外,提供了使用这些引物序列以鉴定DP-004114-3事件的试剂盒。
本发明的另外一个实施方式涉及本文所述的DP-004114-3的特定侧翼序列,所述特定侧翼序列可以用来开发针对生物学样品中DP-004114-3的特异性鉴定方法。更具体地,本发明涉及可以用于开发特异性引物和探针的DP-004114-3的5′和/或3′侧翼区。本发明的又一个实施方式涉及用于基于使用此类特异性引物或探针鉴定生物学样品中DP-004114-3的存在的方法。
根据本发明的另一个实施方式,提供了检测样品中对应玉米事件DP-004114-3的DNA的存在的方法。此类方法包括:(a)使包含DNA的样品与DNA引物组接触,当用于具有从玉米事件DP-004114-3提取的基因组DNA的核酸扩增反应中时,所述DNA引物组产生判定玉米事件DP-004114-3的扩增子;(b)进行核酸扩增反应,从而产生所述扩增子;以及(c)检测所述扩增子。
根据本发明的另一个实施方式,提供了检测样品中对应DP-004114-3事件的DNA分子的存在的方法,此类方法包括:(a)使包含从玉米植物提取的DNA的样品与DNA探针分子接触,所述DNA探针分子在严格杂交条件下与从玉米事件DP-004114-3提取的DNA杂交并且在所述严格杂交条件下不与对照玉米植物DNA杂交;(b)使样品和探针经历严格杂交条件;和(c)检测所述探针与所述DNA的杂交。更具体地,提供了检测样品中对应DP-004114-3事件的DNA分子的存在的方法,此类方法包括:(a)使包含从玉米植物提取的DNA的样品与DNA探针分子接触,所述DNA探针分子由对于该事件为独特的序列(例如连接序列)组成,其中所述DNA探针分子在严格杂交条件下与从玉米事件DP-004114-3提取的DNA杂交并且在所述严格杂交条件下不与对照玉米植物DNA杂交;(b)使样品和探针经历严格杂交条件;以及(c)检测所述探针与所述DNA的杂交。
此外,提供了用于鉴定生物学样品中的事件DP-004114-3的试剂盒和方法,其检出DP-004114-3的特异性区域
提供了包含DP-004114-3的至少一个连接序列的DNA分子;其中连接序列跨越插入基因组中的异源DNA与来自玉米细胞的分布在插入位点侧翼的DNA(即侧翼DNA)之间的接点并且用于判定DP-004114-3事件。
根据本发明的另一个实施方式,提供了产生抗昆虫性玉米植物的方法,所述方法包括以下步骤:(a)将包含赋予抗昆虫性的本发明表达盒的第一亲本玉米系和缺少抗昆虫性的第二亲本玉米系有性杂交,从而产生多个子代植物;以及(b)选择抗昆虫的子代植物。此类方法可以任选地包括又一步骤:将所述子代植物与第二亲本玉米系回交以产生抗昆虫的纯种玉米植物。
本发明的又一个实施方式提供了产生抵抗昆虫的玉米植物的方法,所述方法包括用DNA构建体PHP27118转化玉米细胞,将转化的玉米细胞培育成玉米植物,选择显示抗昆虫性的玉米植物,以及进一步将所述玉米植物培育成能育玉米植物。所述能育玉米植物可以自我授粉或与相容性玉米品种杂交以产生抗昆虫性子代。
本发明的另一个实施方式还涉及用于鉴定生物学样品中玉米事件DP-004114-3的DNA检测试剂盒。该试剂盒包括用于PCR鉴定方案中的第一引物和第二引物,所述第一引物特异性识别DP-004114-3的5′或3′侧翼区,所述第二引物特异性识别在DP-004114-3的外来DNA内部或在所述侧翼DNA内部的序列。本发明的又一个实施方式涉及用于鉴定生物学样品中事件DP-004114-3的试剂盒,所述试剂盒包括具有对应下述序列或与下述序列互补的序列的特异性探针,所述序列与事件DP-004114-3的特定区域具有80%至100%的序列同一性。该探针的序列对应包含事件DP-004114-3的部分5′或3′侧翼区的特定区域。
由本发明实施方式涵盖的方法和试剂盒可以用于不同目的,如,但不限于以下目的:鉴定植物、植物材料中或产品(如但不限于包含或源自植物材料的(新鲜或加工)食品或饲料产品)中的事件DP-004114-3;额外地或备选地,出于分离转基因材料和非转基因材料的目的,所述方法和试剂盒可以用来鉴定转基因植物材料;额外地或备选地,所述方法和试剂盒可以用来确定包含玉米事件DP-004114-3的植物材料的品质。所述试剂盒也可以含有为执行该检测方法所必需的试剂和材料。
本发明的又一个实施方式涉及DP-004114-3玉米植物或其部分,包括、但不限于玉米植物DP-004114-3及源自其的子代的花粉、胚珠、营养细胞、花粉细胞的核和卵细胞的核。DNA引物分子从中产生特定扩增子产物的DP-004114-3玉米植物和种子是本发明的一个实施方式。
本发明的前述和其他方面将会因以下详细描述和附图变得更显而易见。
附图说明
图1.带有所示遗传元件和Hind III限制性酶位点的质粒PHP27118的示意图。质粒大小是54910bp(碱基对)。
图2.显示cry1F、cry34Ab1、cry35Ab1和pat基因(箭头)连同它们的相应调节元件的T-DNA的示意图。显示了T-DNA内部的Hind III限制性酶位点。T-DNA的大小是11978bp。
图3.DP-004114-3的转化和发育的示意图。
图4.在相同遗传背景(DP-004114-3玉米,等位基因系作为阴性对照)下,在使用玉米杂种籽苗的亚致死性籽苗测定法中,西方玉米根虫(WCRW)幼虫发育作用。结果基于三次重复。各直方图显示在卵孵化17日处于三个龄期中每一龄期的幼虫的百分数。向龄期3偏移表示功效下降。
图5.4114玉米中经测序的插入区和基因组边界区的示意图。该图显示从4114玉米基因组DNA产生的经克隆和测序的PCR片段:片段A至F。垂直的虚线代表基因组边界/插入接点。片段G和H分别代表5′和3′基因组边界区。图未按比例绘制。
具体实施方式
提供以下定义和方法以更好地限定本发明并引导本领域普通技术人员实施本发明。除非另外说明,否则术语将根据相关领域普通技术人员的常规用法进行理解。分子生物学中常见术语的定义也可以在Rieger等人,Glossary of Genetics:Classical andMolecular,第5版,Springer-Verlag;New York,1991;和Lewin,Genes V,OxfordUniversity Press:New York,1994中找到。使用如在37 CFR§1,822处所述的DNA碱基命名。
下表描述本文件通篇范围内和尤其是实施例部分中所用的缩写。
缩写表
本公开的组合物包括以专利保藏号PTA-11506保藏的种子及源自其中的植物、植物细胞和种子。申请人已经在2010年11月24日于美国典型培养物保藏中心(ATCC),Manassas,VA 20110-2209USA保藏了玉米事件DP-004114-3的至少2500粒种子,并且保藏物获得ATCC保藏号PTA-11506,保藏了玉米事件DP-032316-8,并且保藏物获得ATCC保藏号PTA-11507,保藏了玉米事件DP-040416-8,并且保藏物获得ATCC保藏号PTA-11508,保藏了玉米事件DP-043A47-8,并且保藏物获得ATCC保藏号PTA-11509。这些保藏物将在国际承认用于专利程序目的的微生物保存布达佩斯条约的条款下维持。这些保藏物仅为便利于本领域技术人员而保存,并非承认保藏物是在35 U.S.C.§112下所要求的。2010年11月24保藏于ATCC的种子取自Pioneer Hi-Bred International,Inc.,7250NW 62ndAvenue,Johnston,Iowa 50131-1000所维护的保藏物。在本申请待决期间,专利商标局首长和当请求时由该首长决定授权的人可以取得这种保藏物。一旦本申请中的任何权利要求得到许可,根据37C.F.R.§1.808,申请人将使公众可获得保藏于美国典型培养物保藏中心(ATCC),10801University Boulevard,Manassas,VA 20110-2209的至少2500粒杂交玉米种子的保藏物样品。玉米事件DP-004114-3的种子的这种保藏物将在作为一家公共保藏单位的ATCC保藏单位维持30年时间,或在最近请求后5年,或持续至本专利的有效期限(这将是更长时间),并且如果在这个时间期间它变得无活力的话将予以更换。另外,申请人已经满足37 C.F.R.§§1,801-1,809的全部要求,包括提供该样品在保藏时有活力的指示。申请人没有权力放弃法律对生物材料转移或其商业运输所规定的任何限制。申请人不豁免对本专利下所授予的权利或在植物品种保护法(7 USC 2321et seq.)下适用于事件DP-004114-3的权利的任何侵犯。禁止未授权的种子繁殖。种子可以接受管理。
如本文所用,术语“包含”意指“包括但不限于”。
如本文所用,术语“玉米”意指玉蜀黍或玉米(maize),包括可以与玉米交配的全部植物品种,包括野生玉米物种。
如本文所用,术语“DP-004114-3特异性”指适于区别性鉴定植物、植物材料中或产品(如但不限于包含或源自植物材料的(新鲜或加工)食品或饲料产品)中事件DP-004114-3的核苷酸序列。
如本文所用,术语“抗昆虫性”和“影响病害昆虫”指在任何发育阶段引起在昆虫采食、生长、和/或行为方面的变化,包括但不限于:杀死昆虫;迟滞生长;阻碍繁殖能力;抑制采食;等。
如本文所用,术语“杀虫活性”和“杀昆虫活性”同义地用来指某生物体或物质(例如蛋白质)的可以通过众多参数进行测量的活性,所述参数包括、但不限于:害虫死亡率、害虫重量减轻、害虫吸引、害虫排斥性和害虫在采食和/或暴露于该生物体或物质持续适宜的时间长度后的其他行为和身体变化。例如,“杀虫蛋白”是本身或与其他蛋白质组合表现杀虫活性的蛋白质。
“编码序列”指编码特定氨基酸序列的核苷酸序列。如本文所用,在指定核酸的语境中使用时,术语“编码”或“编码的”意指所述核酸包含了指导核苷酸序列翻译成所指定蛋白质的必要信息。据以编码蛋白质的信息是通过使用密码子来确定的。编码蛋白质的核酸可以包含在该核酸的翻译区内的非翻译序列(例如内含子)或可以缺少这种居间的非翻译序列(例如,如在cDNA中)。
“基因”指表达特定蛋白质的核酸片段,包括位于编码序列之前(5′非编码序列)和之后(3′非编码序列)的调节序列。“天然基因”指如自然界中找到那样具有其自身调节序列的基因。“嵌合基因”指不是天然基因、且包含在自然界中不在一起的调节序列和编码序列的任何基因。因此,嵌合基因可以包含源自不同来源的调节序列和编码序列,或者源自相同来源、但是以不同于自然界中存在的方式排列的调节序列和编码序列。“内源基因”指在生物体基因组中其天然位置内的天然基因。“外来”指通常不存在于目的位置内的材料。因而“外来DNA”可以包括植物的重组DNA以及新导入的重排DNA。“外来”基因指通常不存在于宿主生物体中、但通过基因转移导入该宿主生物体中的基因。外来基因可以包括插入非天然生物体中的天然基因或嵌合基因。“转基因”是已经通过转化方法导入基因组中的基因。植物基因组中已经插入重组DNA的位点可以称作“插入位点”或“靶位点”。
如本文所用,“插入DNA”指在用来转化植物材料的表达盒内部的异源DNA,而“侧翼DNA”可以作为生物体如植物中天然存在的基因组DNA或经转化方法导入的相对原始插入DNA分子为外源的外来(异源)DNA(例如,与转化事件相关的片段)而存在。如本文所用的“侧翼区”或“侧翼序列”指至少20bp、优选地至少50bp和至多到5000bp的序列,其紧邻原始外来插入DNA分子上游存在并与其保持连续或紧邻原始外来插入DNA分子下游存在并与其保持连续。导致外来DNA随机整合的转化方法将产生这样的转化体,它们含有作为每种转化体的特征并且为其独有的不同侧翼区。当重组DNA经传统杂交导入植物时,其侧翼区通常将不改变。转化体还将在一片异源插入DNA和基因组DNA或两片(2)基因组DNA或两片(2)异源DNA之间含有独特接点。“接点”是两个(2)特定DNA片段接合的点。例如,插入DNA接合侧翼DNA之处存在的接点。接点也存在于其中两个(2)DNA片段以不同于天然生物体中存在的方式接合在一起的转化生物体中。“接点DNA”指包含接点的DNA。本公开中所述的两个连接序列是在玉米基因组DNA和插入物的5′末端之间的如SEQ ID NO:27中所述的接点和在该插入物的3′末端和玉米基因组DNA之间的如SEQ ID NO:28中所述的接点。
如本文所用,针对核酸的“异源”为起源于外来物种的核酸,或者,如果起源于相同物种的话,则为通过蓄意的人为干预对其天然形式在组成和/或基因组基因座方面进行了实质性修饰的核酸。例如,与异源核苷酸序列可操作地连接的启动子所来自的物种可以不同于衍生该核苷酸序列的物种,或,如果来自相同物种,则该启动子不天然地与该核苷酸序列可操作地连接。异源蛋白质可以源自外来物种,或如果源自相同物种,则为通过蓄意的人为干预对其原始形式进行了实质性修饰。
“调节序列”指位于编码序列上游(5′非编码序列)、内部或下游(3′非编码序列)并且影响所连接编码序列的转录、RNA加工或稳定性、或翻译的核苷酸序列。调节序列可以包括启动子、翻译前导序列、内含子、和多腺苷化作用识别序列。
“启动子”指能够控制编码序列或功能性RNA的表达的核苷酸序列。通常,编码序列位于启动子序列的3′。启动子序列由近端上游元件和更远的上游元件组成,后一类元件经常称作增强子。因此,“增强子”是这样的核苷酸序列,所述核苷酸序列可以刺激启动子活性并且可以是启动子的固有元件或经插入以增强启动子的水平或组织特异性的异源元件。启动子可以完全源自天然基因,或由源自自然界中存在的不同启动子的不同元件组成,或甚至包含合成性核苷酸区段。本领域技术人员应当理解,不同的启动子可以引导基因在不同组织或细胞类型中或在不同的发育阶段或应答于不同的环境条件时表达。引起核酸片段在大部分细胞类型中在大多数时间表达的启动子常称作“组成型启动子”。不断发现可用于植物细胞中的多种类型的新启动子;众多实例可以在Okamuro和Goldberg(1989)Biochemistry of Plants 15:1-82的汇编中找到。还认识到,由于在大多数情况下调节序列的确切界限仍未彻底限定,因此不同长度的核酸片段可以具有相同的启动子活性。
“翻译前导序列”指位于基因启动子序列和编码序列之间的核苷酸序列。翻译前导序列存在于翻译起始序列上游的充分加工的mRNA中。翻译前导序列可以影响众多参数,包括初级转录物至mRNA的加工、mRNA稳定性和/或翻译效率。已经描述了翻译前导序列的实例(Turner和Foster(1995)Mol.Biotechnol.3:225-236)。
“3′非编码序列”指位于编码序列下游和包括多腺苷化作用识别序列和编码调节信号的其他序列的核苷酸序列,其中所述调节信号能够影响mRNA加工或基因表达。多腺苷酸化信号通常以影响添加多腺苷酸串至mRNA前体3′末端为特征。不同的3′非编码序列的用途由Ingelbrecht等人,(1989)Plant Cell 1:671-680例举。
“蛋白质”或“多肽”是以编码多肽的多核苷酸中编码序列所决定的特定顺序排列的氨基酸链。
DNA构建体是被连接在一起的产生一个或多个表达盒的DNA分子的装配物。DNA构建体可以是能够在细菌细胞中自我复制并且含有可用于导入DNA分子的多个核酸内切酶限制性位点的质粒,所述DNA分子提供功能性遗传元件即启动子、内含子、前导序列、编码序列、3′终止区及其他;或DNA构建体可以是DNA分子的线性装配物,如表达盒。包含在DNA构建体内的表达盒包括提供信使RNA转录的必需的遗传元件。可以将表达盒设计成在原核细胞或真核细胞中表达。将本发明实施方式的表达盒设计成在植物细胞中表达。
在用于目的生物体中表达的表达盒中提供本发明实施方式的DNA分子。该盒将包括与编码序列可操作地连接的5′和3′调节序列。“可操作地连接”意指连接的核酸序列是连续的,并且在需要接合两个蛋白质编码区的情况下,是连续并且处于相同的读框中。“可操作地连接”意指表示启动子与第二序列之间的功能性连接,其中所述启动子序列启动并且介导与第二序列相对应的DNA序列的转录。该盒可以额外地含有至少一个待共转化入生物体中的额外基因。备选地,可以在多个表达盒上提供额外的基因或多个DNA构建体。
该表达盒将在5′至3′转录方向上包括:在充当宿主的生物体中有功能的转录及翻译起始区域、编码区和转录及翻译终止区。转录起始区域(即,启动子)可以是天然的或类似的,或相对于宿主生物体是外来或异源的。另外,启动子可以是天然序列或备选地是合成序列。表达盒可以在表达盒构建体中额外含有5′前导序列。此类前导序列可起到增强翻译的作用。
应当理解,如本文所用的术语“转基因的”包括任何细胞、细胞系、愈伤组织、植物部分、或植物,其基因型已经因异源核酸的存而改变,包括最初如此改变的那些转基因以及通过有性杂交或无性繁殖从初始转基因产生的那些转基因。本文所用的术语“转基因”不涵盖通过常规植物育种方法或通过自然发生的事件(如随机异花受精、非重组病毒感染、非重组细菌转化、非重组转座或自发突变)进行的基因组(染色体或染色体外)变更。
通过以下方式产生转基因“事件”:用异源DNA构建体(包括包含目的转基因的核酸表达盒)转化植物细胞,由于该转基因插入到该植物的基因组中而再生出一批植株,以及选择以插入到特定基因组位置中为特征的特定植物。事件通过该转基因的表达在表型上(phenotypically)得以表征。在基因层次上看,事件是植物的基因构成(genetic makeup)的一部分。术语“事件”还指通过转化体和包含异源DNA的另一个品种之间有性远交所产生的子代。甚至与轮回亲本反复回交后,插入的DNA和来自转化亲本的侧翼DNA存在于杂交子代中相同的染色体位置处。术语“事件”也指来自原始转化体的包含所插入DNA和与插入DNA紧邻的侧翼序列的DNA,其中预期所述的DNA转移至子代,所述子代因包括该插入DNA的一个亲本系(例如,原始转化体和因自交产生的子代)与不含所述插入DNA的亲本系的有性杂交而接受包含目的转基因的插入DNA。
可以通过以下方式繁育抗昆虫性DP-004114-3玉米植物:首先将由培育自转基因DP-004114-3玉米植物及其子代的玉米植物组成的第一亲本玉米植物与缺少抗昆虫性的第二亲本玉米植物有性杂交,从而产生多个第一子代植物,其中所述转基因DP-004114-3玉米植物及其子代源自用赋予抗昆虫性的本发明实施方式的表达盒转化;随后选择抵抗昆虫的第一子代植物;将第一子代植物自交,从而产生多个第二子代植物;以及随后从第二子代植物选择抗昆虫性植物。这些步骤可以进一步包括将第一抗昆虫性子代植物或第二抗昆虫性子代植物与第二亲本玉米植物或第三亲本玉米植物回交,从而产生抵抗昆虫的玉米植物。
如本文所用,术语“植物”包括指称完整植物、植物器官(例如,叶、茎、根等)、种子、植物细胞及其子代。应理解为处于本发明范围内的转基因植物的部分包括,例如起源于先前用本发明DNA分子转化的转基因植物或其子代并且因此至少部分地由转基因细胞组成的植物细胞、原生质体、组织、愈伤组织、胚以及花、茎、果实、叶子、和根,它们也是本发明的实施方式。
本文所用的术语“植物细胞”包括但不限于种子、悬浮培养物、胚、分生组织区、愈伤组织、叶、根、芽苗、配子体、孢子体、花粉、和小孢子。可用于本发明方法的植物的类别通常与适于转化技术的高等植物的类别一样宽泛,包括单子叶植物和双子叶植物在内。
“转化”指核酸片段转移至宿主生物体的基因组中,从而得到遗传上稳定的遗传性。含有转化的核酸片段的宿主生物体称作“转基因”生物体。植物转化方法的实例包括农杆菌介导的转化法(De Blaere等人,(1987)Meth.Enzymol.143:277)和粒子加速或“基因枪”转化技术(Klein等人,(1987)Nature(London)327:70-73;美国专利No.4,945,050,所述文献通过引用方式并入本文)。下文公开其它转化方法。
因而,可以将本发明分离出的多核苷酸并入能够导入宿主细胞中并在其中复制的重组构建体,一般是DNA构建体。这种构建体可以是载体,其包括能够在给定宿主细胞中转录和翻译编码多肽的序列的复制系统和序列。适用于稳定转染植物细胞或适用于建立转基因植物的众多载体已经在例如Pouwels等人,(1985;Supp.1987)Cloning Vectors:ALaboratory Manual,Weissbach和Weissbach(1989)Methods for Plant MolecularBiology,(Academic Press,New York);和Flevin等人,(1990)Plant Molecular BiologyManual,(Kluwer Academic Publishers)中描述。一般,植物表达载体包括例如一个或多个在5′和3′调节序列的转录性控制下的克隆的植物基因和显性可选择标记。此类植物表达载体还可以含有启动子调节区(例如,控制诱导型表达或组成型表达、环境或发育调节型表达、或者细胞或组织特异性表达的调节区)、转录引发起始位点、核糖体结合位点、RNA加工信号、转录终止位点和/或多腺苷酸化信号。
还应该理解,也可以将两株不同的转基因植株交配以产生含有两个独立分离、添加的外源基因的子代。合适子代的自交可产生针对该两个所加入的外源基因而言均是纯合的植物。还设想与亲本植株的回交和与非转基因植株的远交,还有无性繁殖。有关常用于不同性状和作物的其他育种方法的描述,可在几个参考文献之一中找到,例如Fehr,在Breeding Methods for Cultivar Development,Wilcos J.编著,American Society ofAgronomy,Madison Wis.(1987)中。
“探针”是与常规的可检测标记或报道分子(如放射性同位素、配体、化学发光物质、或酶)连接的分离的核酸。这种探针与靶核酸的链互补,在本发明的情况下,与来自玉米事件DP-004114-3的分离出来的DNA的链互补,无论其是来自玉米植物还是来自包括来自该事件的DNA的样品。根据本发明的探针包含与靶DNA序列特异性结合并且可以用来检测这种靶DNA序列存在的脱氧核糖核酸或核糖核酸和聚酰胺及其他探针材料。
“引物”是分离的核酸,其通过核酸杂交与互补性靶DNA链退火以形成引物和靶DNA链之间的杂交体,随后由聚合酶(如DNA聚合酶)沿靶DNA链延伸。本发明的引物对指它们用于扩增靶核酸序列的用途,例如,通过PCR或其他常规的核酸扩增方法。“PCR”或“聚合酶链反应”是用于扩增特定DNA区段的技术(见,美国专利No.4,683,195和No.4,800,159;所述专利通过引用方式并入本文)。
探针和引物要有足够的核苷酸长度,以在操作员确定的杂交条件或反应条件下与靶DNA序列特异性地相结合。该长度可以是任何长度,其是在选择的检测方法中有用的足够长度。通常,使用11个或更多个核苷酸的长度、18个或更多个核苷酸的长度、和22个或更多个核苷酸的长度。此类探针和引物在高严格性杂交条件下与靶序列特异性杂交。根据本发明实施方案的探针和引物可以与靶序列具有完全的连续核苷酸DNA序列相似性,不过可以通过常规方法设计与靶DNA序列不同并保持与靶DNA序列杂交的能力的探针。探针可以作为引物使用,不过通常设计成与靶DNA或RNA结合并且不用于扩增过程中。
特异性引物可以用来扩增整合片段以产生可以作为鉴定生物学样品中事件DP-004114-3的“特异性探针”使用的扩增子。当探针与生物学样品的核酸在允许探针与该样品结合的条件下杂交时,这种结合可以被检测到并因而允许指示事件DP-004114-3在生物学样品中存在。已经在本领域中描述了对结合的探针的这种鉴定。在本发明的一个实施方式中,所述特异性探针是这样的序列,该序列在优化条件下与所述事件的5′或3′侧翼区内部的区域特异性杂交并且还包含与该区域连续的外来DNA的部分。特异性探针可以包含与该事件的特定区域至少80%、80%至85%、85%至90%、90%至95%、和95%至100%相同(或互补)的序列。
用于制备和使用探针和引物的方法例如在Sambrook等人,Molecular Cloning:ALaboratory Manual,第2版,第1-3卷,Cold Spring Harbor Laboratory Press,ColdSpring Harbor,N.Y.1989(下文为“Sambrook等人,1989”);Ausubel等人编著,CurrentProtocols in Molecular Biology,Greene Publishing和Wiley-Interscience,NewYork,1995(定期更新)(下文为“Ausubel等人,1995”);和Innis等人,PCR Protocols:AGuide to Methods and Applications,Academic Press:San Diego,1990中描述。PCR引物对可以从已知的序列衍生,例如,通过使用意图用于此目的的计算机程序如Vector NTI版本6中的PCR引物分析工具(Informax Inc.,Bethesda MD);PrimerSelect(DNASTAR Inc.,Madison,WI);和Primer(版本1991,Whitehead Institute for BiomedicalResearch,Cambridge,Mass.)。另外,还可以目视地扫描序列,并可使用本领域技术人员已知的指南手工地确定引物。
如本文中所用的“试剂盒”指一组试剂,它们意在实施本发明的方法实施方式,更具体地是鉴定生物学样品中的事件DP-004114-3。本发明的试剂盒可以用于以下目的,并且其组分可以为以下目的而专门调整:质量控制(例如,种子批的纯度)、检测植物材料或包含或源自植物材料的材料(如但不限于食品或饲料产品)中的事件DP-004114-3。如本文中所用,“植物材料”指从植物获得或衍生的材料。
通过常规方法例如通过将此类序列再克隆和测序,基于本文中公开的侧翼DNA和插入物序列的引物和探针可以用来证实(并且,根据需要,校正)公开的序列。本发明的核酸探针和引物在严格条件下与靶DNA序列杂交。可以用任何常规的核酸杂交或扩增方法来鉴定样品中来自转基因事件的DNA的存在。核酸分子或其片段能够在某些环境下与其他核酸分子特异性地杂交。如本文所用,如果两个核酸分子能够形成反平行双链核酸结构,则称这两个分子能够彼此特异性杂交。
如果两个核酸分子显示出完全互补性,则称其中一个核酸分子是另一个核酸分子的“互补物”。如本文所用,当某分子的每一个核苷酸均与另一个分子的核苷酸互补时,则称该分子显示出“完全互补性”。如果两个分子可以在至少常规“低严格性”条件下以足够稳定性彼此杂交,并它们仍为无内应力(退火)的,则称这两个分子是“最小互补的”。类似地,如果两个分子可以以允许它们在常规“高严格性”条件下保持彼此无内应力且足够稳定地彼此杂交,则称这两个分子是“互补的”。常规的严格性条件由Sambrook等人,1989并且由Haymes等人,在:Nucleic Acid Hybridization,a Practical Approach,IRL Press,Washington,D.C.(1985)中描述,偏离完全互补性因此是允许的,只要此类偏离没有彻底地排除分子形成双链结构的能力。为了充当引物或探针,核酸分子仅需要在序列上足够互补以能够在所用的特定溶剂和盐浓度下形成稳定的双链结构。
在杂交反应中,特异性通常决定于杂交后的洗涤,关键因素为最终洗涤溶液的离子强度和温度。热解链温度(Tm)是(在限定的离子强度和pH时)50%互补性靶序列与完全匹配的探针杂交的温度。对于DNA-DNA杂交体,Tm可以从Meinkoth和Wahl(1984)Anal.Biochem.138:267-284的等式近似获得:Tm=81.5℃+16.6(log M)+0.41(%GC)-0.61(%form)-500/L;其中M为单价阳离子的摩尔浓度,%GC为DNA中鸟嘌呤核苷酸和胞嘧啶核苷酸的百分数,%form为杂交溶液中甲酰胺的百分数,L为杂交体的长度(单位为碱基对)。Tm因每1%错配下降约1℃;因此,可以调节Tm、杂交、和/或洗涤条件以与具有所需同一性的序列杂交。例如,如果寻求具有>90%同一性的序列,则可将Tm降低10℃。通常,将严格条件选择为比特定序列及其互补序列在确定的离子强度和pH下的Tm低约5℃。然而,极严格条件可以利用比Tm低1、2、3或4℃的杂交和/或洗涤;中等严格条件可以利用比Tm低6、7、8、9或10℃的杂交和/或洗涤;低严格条件可以利用比Tm低11、12、13、14、15或20℃的杂交和/或洗涤。
利用该公式,杂交和洗涤组成以及所需的Tm,普通技术人员将认识到,杂交和/或洗涤溶液的严格性的变化固有地得到了描述。如果所需的错配程度导致Tm低于45℃(水溶液)或32℃(甲酰胺溶液),则优选增加SSC浓度以使得可以使用更高的温度。对核酸杂交的广泛指导存在于Tijssen(1993)Laboratory Techniques in Biochemistry andMolecular Biology-Hybridization with Nucleic Acid Probes,第I部分,第2章(Elsevier,New York);和Ausubel等人编著(1995)和Sambrook等人,(1989)中。
如本文所用,基本上同源的序列是这样的核酸分子,其将在高严格条件下特异性杂交至与其比较的核酸分子互补物。促进DNA杂交的适宜严格性条件,例如,约45℃下的6X氯化钠/柠檬酸钠(SSC),随后50℃下2XSSC洗涤,是本领域技术人员已知的,或可以在Ausubel等人,(1995),6.3.1-6.3.6中找到。通常,严格条件将为其中盐浓度低于约1.5M钠离子,通常为约0.01至1.0M钠离子浓度(或其他盐),pH为7.0至8.3,对短探针(例如,10至50个核苷酸)而言温度为至少30℃,对长探针(例如超过50个核苷酸)而言温度为至少约60℃的那些条件。严格条件还可通过添加去稳定剂如甲酰胺来实现。示例性的低严格性条件包括在用30%至35%甲酰胺、1M NaCl、1%SDS(十二烷基硫酸钠)的缓冲溶液37℃下杂交,并在1X至2X SSC(20X SSC=3.0M NaCI/0.3M柠檬酸三钠)中在50℃至55℃下洗涤。示例性的中等严格性条件包括在40%至45%甲酰胺、1M NaCl、1%SDS中在37℃下杂交和在0.5X至1XSSC中在55℃至60℃下洗涤。示例性的高严格性条件包括在50%甲酰胺、1M NaCl、1%SDS中在37℃下杂交和在0.1X SSC中在60℃至65℃下洗涤。本发明的核酸可以在中等严格条件下与一个或多个对DP-004114-3事件为独特的核酸分子或其互补物、或它们任一者的片段特异性地杂交。
将序列进行比对以作比较的方法是本领域公知的。因此,可以使用计算机数学算法完成任意两个序列之间同一性百分比的确定。此类数学算法的非限制性实例是Myers和Miller(1988)CABIOS 4:11-17的算法;Smith等人,(1981)Adv.Appl.Math.2:482的局部同源性算法;Needleman和Wunsch(1970)J.Mol.Biol.48:443-453的同源性比对算法;Pearson和Lipman(1988)Proc.Natl.Acad.Sci.85:2444-2448的检索相似性方法;Karlin和Altschul(1990)Proc.Natl.Acad.Sci.USA 87:2264的算法,如Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877中那样修正后的算法。
可利用这些数学算法的计算机程序进行序列的比较,从而确定序列同一性。此类程序包括、但不限于:PC/Gene程序(从Intelligenetics,Mountain View,California可获得)中的CLUSTAL;ALIGN程序(2.0版本);ALIGN PLUS程序(版本3.0,版权1997);和Wisconsin Genetics软件包,版本10(从Accelrys,9685Scranton Road,San Diego,CA92121,USA可获得)中的GAP、BESTFIT、BLAST、FASTA和TFASTA。使用这些程序的序列比对可用默认参数来进行。
CLUSTAL程序由Higgins和Sharp,Gene 73:237-244(1988);Higgins和Sharp,CABIOS 5:151-153(1989);Corpet,等人,Nucleic Acids Research 16:10881-90(1988);Huang,等人,Computer Applications in the Biosciences 8:155-65(1992)和Pearson,等人,Methods in Molecular Biology 24:307-331(1994)充分描述。ALIGN和ALIGN PLUS程序基于上文的Myers和Miller(1988)算法。Altschul等人,(1990)J.Mol.Biol.215:403的BLAST程序基于上文的Karlin和Altschul(1990)算法。可用于数据库相似性搜索的BLAST家族程序程序包括:BLASTN,用于核苷酸查询序列针对核苷酸数据库序列进行查询;BLASTX,用于核苷酸查询序列针对蛋白质数据库序列进行查询;BLASTP,用于蛋白质查询序列针对蛋白质数据库序列进行查询;TBLASTN,用于蛋白质查询序列针对核苷酸数据库序列进行查询;和TBLASTX,用于核苷酸查询序列针对核苷酸数据库序列进行查询。见,Ausubel,等人,(1995)。比对可以通过视检法手工地进行。
为获得用于比较目的空位比对结果,空位BLAST(在BLAST 2.0中)可以如Altschul等人,(1997)Nucleic Acids Res.25:3389中所述那样使用。或者,可使用PSI-BLAST(在BLAST 2.0中)来进行迭代搜索,该搜索可检测分子之间的远源关系。见上文Altschul等人,(1997)。当采用BLAST、Gapped BLAST、PSI-BLAST时,可使用各个程序的默认参数(例如BLASTN用于核苷酸序列,BLASTX用于蛋白质)。
如本文所用,在两个核苷酸序列或多肽序列的场合下,“序列同一性”或“同一性”意指在对指定的比较窗口范围内的最大对应性进行比对时,这两个序列中残基的相同程度。当把序列同一性百分数运用于蛋白质时,应认识到,不相同的残基位置往往差别仅在于保守氨基酸发生了置换,其中氨基酸残基被其他具有相似的化学性质(例如电荷或疏水性)的氨基酸残基所置换,因此不会改变分子的功能性质。当序列差别在于保守置换,则可上调百分比序列同一性,以对此种置换的保守性质进行校正。将由于此类保守性置换而不同的序列称作具有“序列相似性”或“相似性”。用于进行这种调整的方法是本领域技术人员熟知的。通常,这涉及将保守置换打分为部分错配而不是完全错配,从而提高序列同一性百分数。因而,例如,如果相同的氨基酸给予1分,非保守置换给予0分,则保守置换给予0至1之间的分数。保守置换的打分是例如如在程序PC/GENE(Intelligenetics,Mountain View,California)中所执行那样进行计算。
本文所用的“序列同一性百分数”意指通过在比较窗口上比较两个最佳比对的序列所确定的数值,其中多核苷酸序列在比较窗口中的部分与参考序列(不包含添加或缺失)相比包含添加或缺失(即空位),以便两个序列的最佳比对。该百分数是这样计算的:确定在两个序列中出现相同核酸碱基或氨基酸残基的位置的数目以得到匹配的位置的数目,将匹配的位置的数目除以比较窗口中的位置的总数目,然后将结果乘以100以得到序列同一性百分数。
就使用特定扩增引物扩增靶核酸序列(例如,通过PCR)而言,“严格条件”是允许所述引物对仅与靶核酸序列杂交并且优选地允许在DNA热扩增反应中产生特定扩增产物即扩增子的条件,其中具有相应野生型序列(或其互补序列)的引物将与所述靶核酸序列结合。
术语“对靶序列特异的”表示探针或引物在严格杂交条件下仅与包含括靶序列的样品中的靶序列杂交。
如本文所用,“扩增的DNA”或“扩增子”指作为核酸模板的部分的靶核酸序列的核酸扩增产物。例如,为确定因有性杂交产生的玉米植物是否含有来自本发明玉米植物的转基因事件基因组DNA,可以使从玉米植物组织样品提取的DNA接受使用DNA引物对的核酸扩增方法处理,以产生判定所述事件DNA存在的扩增子,其中所述DNA引物对包括第一引物和第二引物,所述第一引物源自与插入的异源DNA的插入位点相邻的侧翼序列,所述第二引物源自插入的异源DNA。备选地,第二引物可以源自侧翼序列。所述扩增子具有还可据以判定所述事件的长度和序列。扩增子的长度可以在所述引物对外加一个核苷酸碱基对的联合长度至通过DNA扩增方案可产生的任何扩增子长度的范围内。备选地,引物对可以源自插入的DNA两侧的侧翼序列,从而产生这样的扩增子,所述扩增子包括PHP27118表达构建体的完整插入物核苷酸序列以及在转基因插入物侧翼的序列。源自侧翼序列的引物对的成员可以与插入的DNA序列相隔一段距离存在,这个距离的范围可以是1个核苷酸碱基对直至扩增反应的限值,或是约20,000bp。术语“扩增子”的使用特别地排除了可以在DNA热扩增反应中形成的引物二聚体。
核酸扩增可以通过本领域已知的多种核酸扩增方法(包括PCR)中任一种来完成。多种扩增方法是本领域已知的并且尤其在美国专利No.4,683,195和No.4,683,202和在上文Innis等人(1990)中描述。已经开发了PCR扩增方法以扩增多到22Kb的基因组DNA和多到42Kb的噬菌体DNA(Cheng等人,Proc.Natl.Acad.Sci.USA 91:5695-5699,1994)。这些方法以及本领域已知扩增DNA的其他方法可以用于本发明实施方式的实施中。应当理解:特定PCR方案中的众多参数可能需要针对具体的实验室条件进行调整并且可以略作修改并仍允许获得相似的结果。这些调整对本领域技术人员是显而易见的。
可以通过多项技术检测出由这些方法产生的扩增子,所述技术包括、但不限于遗传比特分析法(Nikiforov,等人,Nucleic Acid Res.22:4167-4175,1994),其中设计了与相邻的侧翼DNA序列和插入的DNA序列均重叠的DNA寡核苷酸。将该寡核苷酸固定于微量多孔平板的孔中。在对扩增目的区进行了PCR反应(用一个位于插入序列内的引物和一个位于相邻的侧翼序列内的引物)后,单链PCR产物可以与固定的寡核苷酸杂交并且充当单碱基延伸反应的模板,所述单碱基延伸反应使用DNA聚合酶和对下一个预期碱基特异的标记的ddNTP。读出可以是荧光的或基于ELISA的。信号指示出因成功扩增、杂交、和单碱基延伸而存在插入物/侧翼序列。
另一个检测方法是如Winge(2000)Innov.Pharma.Tech.00:18-24描述的焦磷酸测序技术。在这个方法中,设计了与相邻DNA和插入DNA接点重叠的寡核苷酸。将所述寡核苷酸与来自目的区域(一个位于插入序列内的引物和一个位于侧翼序列内的引物)的单链PCR产物杂交并且在DNA聚合酶、ATP、硫酸化酶、萤光素酶、腺苷三磷酸双磷酶、腺苷5′磷酸硫酸盐和萤光素存在下孵育。单独添加dNTP并且该掺入产生可测量的光信号。光信号指示因成功扩增、杂交和单碱基或多碱基延伸而存在转基因插入物/侧翼序列。
如Chen等人,(1999)Genome Res.9:492-498描述的荧光偏振法也是可以用来检测本发明扩增子的方法。使用这种方法时,设计与侧翼和插入DNA接点重叠的寡核苷酸。将所述寡核苷酸与来自目的区域(一个位于插入的DNA内的引物和一个位于侧翼DNA序列内的引物)的单链PCR产物杂交并且在DNA聚合酶和荧光标记的ddNTP存在下孵育。单碱基延伸导致该ddNTP的掺入。掺入可以使用荧光计度量为偏振的变化。偏振的变化指示因成功扩增、杂交和单碱基延伸而存在转基因插入物/侧翼序列。
(PE Applied Biosystems,Foster City,Calif.)被描述为检测和量化DNA序列存在的方法并且在制造商提供的说明书中充分解释。简而言之,设计了与侧翼和插入DNA接点重叠的FRET寡核苷酸探针。FRET探针和PCR引物(一个位于插入DNA序列内的引物和一个位于侧翼基因组内的引物)在热稳定聚合酶和dNTP存在下循环。FRET探针的杂交导致切割荧光部分并且使其释放远离FRET探针上的淬灭部分。荧光信号指示因成功扩增和杂交而存在侧翼/转基因插入物序列。
已经描述了用于序列检测的分子信标,如Tyangi等人,(1996)NatureBiotech.14:303-308中所述。简而言之,设计了与侧翼和插入DNA接点重叠的FRET寡核苷酸探针。FRET探针的独特结构导致它含有使得荧光部分和淬灭部分紧密靠近的二级结构。FRET探针和PCR引物(一个位于插入DNA序列内的引物和一个位于侧翼序列内的引物)在热稳定聚合酶和dNTP存在下循环。在成功PCR扩增后,FRET探针与靶序列的杂交导致消除探针二级结构以及荧光部分和淬灭部分的空间分隔。荧光信号产生。荧光信号指示因成功扩增和杂交而存在侧翼/转基因插入物序列。
使用对扩增子内部存在的序列特异的探针的杂交反应是用来检测由PCR反应产生的扩增子的又一个方法。
玉米事件DP-004114-3能有效对抗昆虫害虫,所述昆虫害虫包括选自鞘翅目(Coleoptera)、双翅目(Diptera)、膜翅目(Hymenoptera)、鳞翅目(Lepidoptera)、食毛目(Mallophaga)、同翅目(Homoptera)、半翅目(Hemiptera)、直翅目(Orthoptera)、缨翅目(Thysanoptera)、革翅目(Dermaptera)、等翅目(Isoptera)、虱目(Anoplura)、蚤目(Siphonaptera)、毛翅目(Trichoptera)等的昆虫,尤其包括选自鞘翅目和鳞翅目的昆虫。
鳞翅目昆包括、但不限于夜蛾科(Noctuidae)中的夜蛾、地老虎、尺蠖、和实夜蛾:小地老虎(Agrotis ipsilon Hufnagel)(黑地老虎);灰地老虎(A.orthogonia Morrison)(西部地老虎);黄地老虎(A.segetum Denis&Schiffermüller)(蔓青蛾);粒肤地老虎(A.subterranea Fabricius)(粒肤地蚕);叶波纹须蛾(Alabama argillacea Hübner)(棉叶虫);黎豆夜蛾(Anticarsia gemmatalis Hübner)(黎豆毛虫);粗皮夜蛾(Athetismindara Barnes and McDunnough)(粗皮地蚕);棉斑实蛾(Earias insulana Boisduval)(多刺螟蛉(spiny bollworm));翠纹金刚钻(E.vittella Fabricius)(斑点螟蛉(spottedbollworm));柑橘栖夜蛾(Egira(Xylomyges)curialis Grote)(柑橘地老虎);暗缘地老虎(Euxoa messoria Harris)(darksided cutworm);蕃茄夜蛾(Helicoverpa armigera Hübner)(美洲螟蛉(American bollworm));谷实夜蛾(H.zea Boddie)(美洲棉铃虫(cornearworm)或棉铃虫(cotton bollworm));烟芽夜蛾(Heliothis virescens Fabricius)(烟青虫(tobacco budworm));苜蓿绿叶蛾(Hypena scabra Fabricius)(green cloverworm);Hyponeuma taltula Schaus;蓓带夜蛾(Mamestra configurata Walker)(berthaarmyworm);甘蓝夜蛾(M.brassicae Linnaeus)(cabbage moth);斑马纹夜蛾(Melanchrapicta Harris)(zebra caterpillar);小毛胫夜蛾(Mocislatipes Guenée)(small mocismoth));普通夜盗蛾(Pseudaletia unipuncta Haworth)(夜蛾);大豆夜蛾(Pseudoplusiaincludens Walker)(大豆尺蠖);西部豆类夜盗虫(Richia albicosta Smith)(Westernbean cutworm);草地贪夜蛾(Spodoptera frugiperda JE Smith)(秋夜蛾);甜菜夜蛾(S.exigua Hübner)(beet armyworm);斜纹贪夜蛾(S.litura Fabricius)(tobaccocutworm,cluster caterpillar);粉纹夜蛾(Trichoplusia ni Hübner)(卷心菜尺蠖);来自螟蛾科(Pyralidae)和草螟科(Crambidae)的钻心虫、鞘蛾、结网虫(webworm)、球果蛾(coneworm)、和雕叶虫(skeletonizer),如小蜡螟(Achroia grisella Fabricius)(lesserwax moth);脐橙螟蛾(Amyelois transitella Walker)(naval orangeworm);地中海粉螟蛾(Anagasta kuehniella Zeller)(Mediterranean flour moth);粉斑螟蛾(Cadracautella Walker)(almond moth);粉红禾草螟(Chilo partellus Swinhoe)(斑点蛀茎虫(spotted stalk borer));二化螟(C.suppressalis Walker)(条纹蛀茎虫/稻钻心虫(striped stem/rice borer));C.terrenellus Pagenstecher(甘蔗蛀茎虫(sugarcanestem borer));外米缀蛾(Corcyra cephalonica Stainton)(米蛾(rice moth));玉米根草螟(Crambus caligmosellus Clemens)(com root webworm);早熟禾草螟(C.teterrellusZincken)(bluegrass webworm);稻纵卷叶螟(Cnaphalocrocis medinalis Guenée)(稻卷叶虫(rice leaf roller));葡萄野螟(Desmia funeralis Hübner)(葡萄卷叶虫(grapeleaffolder));甜瓜绢野螟(Diaphania hyalinata Linnaeus)(melon worm);D.nitidalisStoll(pickleworm);Diatraea flavipennella Box;西南玉米螟(D.grandiosella Dyar)(西南玉米钻心虫(southwestern corn borer))、小蔗螟(D.saccharalis Fabricius)(甘蔗钻心虫(surgarcane borer));南美玉米苗斑螟(Elasmopalpus lignosellus Zeller)(小玉米茎钻心虫(lesser cornstalk borer));墨西哥稻螟(Eoreuma loftini Dyar)(Mexican rice borer);烟草粉螟(Ephesha elutella Hübner)(烟草(cacao)飞蛾);大蜡螟(Galleria mellonella Linnaeus)(greater wax moth);甘蔗卷叶蛾(Hedyleptaaccepta Butler)(甘蔗卷叶虫);水稻切叶野螟(Herpetogramma licarsisalis Walker)(sod webworm);向日葵同斑螟(Homoeosoma electellum Hulst)(向日葵螟);草地螟(Loxostege sticticalis Linnaeus)(beet webworm);豇豆荚螟(Maruca testulalisGeyer)(bean pod borer);茶树螟(Orthaga thyrisalis Walker)(tea tree web moth);玉米螟(Ostrinia nubilalis Hübner)(欧洲玉米钻心虫(European corn borer));印度谷螟(Plodia interpunctella Hübner)(印度谷蛾(Indian meal moth));三化螟(Scirpophaga incertulas Walker)(yellow stem borer);芹菜网螟(Udea rubigalisGuen6e)(芹菜叶虫(celery leaftier));和卷蛾科(Tortricidae)的卷叶虫、蚜虫、种实虫以及果实虫,西部黑头长翅卷蛾(Tortricidae Acleris gloverana Walsingham)(Westernblackheaded budworm);东部黑头长翅卷蛾(A.variana Fernald)(Eastern blackheadedbudworm);棉褐带卷叶蛾(Adoxophyes orana Fischer von)(summer fruittortrix moth);黄卷蛾属(Archips)物种,包括果树黄卷蛾(A.argyrospila Walker)(果树卷叶虫(fruit tree leaf roller))和欧洲卷叶蛾(A.rosana Linnaeus)(European leafroller);Argyrotaenia属物种;巴西苹果卷叶虫(Bonagota salubricola Meyrick)(Brazilian apple leafroller);色卷蛾属(Choristoneura)物种;条纹向日葵螟(Cochylis hospes Walsingham)(banded sunflower moth);榛小卷蛾(Cydialatiferreana Walsingham)(filbertworm);苹果蠹蛾(C.pomonella Linnaeus)(codlingmoth);萄果实蛀虫(Endopiza viteana Clemens)(grape berry moth);女贞细卷蛾(Eupoecilia ambiguella Hübner)(vine moth);梨小食心虫(Grapholita molestaBusck)(oriental fruit moth);欧洲葡萄小卷叶蛾(Lobesia botrana Denis)&Schiffermüller(European grape vine moth);杂色卷叶蛾(Platynota flavedana Clemens)(variegated leafroller);荷兰石竹小卷蛾(P.stultana Walsingham)(omnivorousleafroller);苹白小卷蛾(Spilonota ocellana Denis)&Schiffermüller(eyespottedbud moth);和向日葵芽蛾(Suleima helianthana Riley)(sunflower bud moth)。
鳞翅目中选择的其他农艺学害虫包括\但不限于秋星尺蠖(Alsophila pometariaHarris)(fall cankerworm);桃枝麦蛾(Anarsia lineatella Zeller)(peach twigborer);犀额蛾(Anisota senatoria J.E.Smith)(orange striped oakworm);柞蚕(Antheraeapernyi Guérin-Méneville)(Chinese Oak Silkmoth);家蚕(Bombyx moriLinnaeus)(Silkworm);棉潜蛾(Bucculatrix thurberiella Busck)(cotton leafperforator);苜蓿黄蝶(Colias eurytheme Boisduval)(alfalfa caterpillar);胡桃黄蝶(Grote&Robinson)(walnut caterpillar);落叶松毛虫(Dendrolimus sibiricusTschetwerikov)(Siberian silk moth),白尺蠖(Ennomos subsignaria Hübner)(elmspanworm);菩提尺蠖(Erannistiliaria Harris)(linden looper);Erechthiasflavistriata Walsingham(sugarcane bud moth);黄毒蛾(Euproctis chrysorrhoeaLinnaeus)(browntail moth);黑拟蛉蛾(Harrisina americana Guérin-Méneville)(grapeleaf skeletonizer);Heliothis subflexa Guenée;牧草天蚕蛾(Hemileucaoliviae Cockrell)(range caterpillar);美国白蛾(Hyphantria cunea Drury)(fallwebworm);番茄蠹蛾(Keiferia lycopersicella Walsingham)(tomato pinworm);二尾蛱蝶(Lambdina fiscellaria fiscellaria Hulst)(Eastern hemlock looper);西部铁杉尺蠖(L.fiscellaria lugubrosa Hulst)(Western hemlock looper);柳毒蛾(Leucomasalicis Linnaeus)(satin moth);舞毒蛾(Lymantria dispar Linnaeus)(gypsy moth);天幕毛虫属(Malacosoma)物种;番茄天蛾(Manduca quinquemaculata Haworth)(fivespotted hawk moth,tomato hornworm);烟草天蛾(Msexta Haworth)(tomato homworm,tobacco hornworm);冬尺蠖(Operophtera brumata Linnaeus)(winter moth);古毒蛾属(Orgyia)物种;春尺蠖(Paleacrita vernata Peck)(spring cankerworm);大凤蝶(Papilio cresphontes Cramer)(giant swallowtail,orange dog);加州槲蛾(Phryganidia californica Packard)(California oakworm);柑橘潜叶蛾(Phyllocnistis citrella Stainton)(citturus leafminer);斑幕潜叶蛾(Phyllonorycter blancardella Fabricius)(spotted tentiform leafminer);大菜粉蝶(Pieris brassicae Linnaeus)(large white butterfly);小菜粉蝶(P.rapae Linnaeus)(small white butterfly);暗脉菜粉蝶(P.napi Linnaeus)(green veinedwhitebutterfly);洋蓟羽蛾(Platyptilia carduidactyla Riley)(artichoke plumemoth);小菜蛾(Plutella xylostella Linnaeus)(diamondback moth);棉红铃虫(Pectinophora gossypiella Saunders)(pink bollworm);南方菜青虫(Pontiaprotodice Boisduval&Leconte)(Southern cabbageworm);Sabulodes aegrotata Guenée(omnivorous looper);红疣天社蛾(Schizura concinna J.E.Smith)(red humpedcaterpillar);麦蛾(Sitotroga cerealella Olivier)(Angoumois grain moth);Telchinlicus Drury(giant sugarcane borer);松异带蛾(Thaumetopoea pityocampaSchiffermuller)(pine processionary caterpillar);结网衣蛾(Tineola bisselliellaHummel)(webbing clothesmoth);番茄斑潜蝇(Tuta aboluta Meyrick)(tomatoleafminer)和苹果巢蛾(Yponomeuta padella Linnaeus)(ermine moth)。
值得关注的是鞘翅目的幼虫和成体,其包括来自长角象科(Anthribidae)、豆象科(Bruchidae)和象甲科(Curculionidae)的象鼻虫,包括、但不限于:棉铃象甲(Anthonomusgrandis Boheman)(boll weevi);向日葵茎象甲(Cylindrocopturus adspersus LeConte)(sunflower stem weevi);蔗根非耳象(Diaprepes abbreviatus Linnaeus)(Diaprepesroot weevi);三叶草叶象(Hypera punctata Fabricius)(clover leaf weevi);稻水象甲(Lissorhoptrus oryzophilus Kuschel)(rice water weevi);西印度蔗象甲(Metamasiushemipterus hemipterus Linnaeus)(West Indian cane weevi);丝光蔗象甲(Mhemipterus sericeus Olivier)(silky cane weevi);谷象(Sitophilus granariusLinnaeus)(granary weevi);米象(S.oryzae Linnaeus)(rice weevi);红色葵花籽象甲(Smicronyx fulvus LeConte)(red sunflower seed weevi);灰色葵花籽象甲(S.sordidus LeConte)(gray sunflower seed weevi);玉米象甲(Sphenophorus maidisChittenden)(maize billbug);S.livis Vaurie(sugarcane weevil);新几内亚蔗象甲(Rhabdoscelus obscurus Boisduval)(New Guinea sugarcane weevi);叶甲科(Chrysomelidae)的跳甲、黄瓜叶甲、根虫、叶甲、马铃薯叶甲以及潜叶虫,包括、但不限于:荒地玉米跳甲(Chaetocnema ectypa Horn)(desert com flea beetle);玉米铜色跳甲(C.pulicaria Melsheimer)(玉米跳甲(corn flea beetle))葡萄肖叶甲(Colaspisbrunnea Fabricius)(grape colaspis);巴氏根叶甲(Diabrotica barberi Smith&Lawrence)(北部玉米根虫(northern corn rootworm));黄瓜十一星叶甲(D.undecimpunctata howardi Barber)(南方玉米根虫(southern corn rootworm));玉米根萤叶甲(D.virgifera virgifera LeConte)(西方玉米根虫(western corn rootworm));科罗拉多州马铃薯甲虫(Leptinotarsa decemlineata Say)(Colorado potato beetle);黑角负泥虫(Oulema melanopus Linnaeus)(禾谷叶甲(cereal leaf beetle));十字花科跳甲(Phyllotreta cruciferae Goeze)(玉米跳甲);向日葵叶甲(Zygogrammaexclamationis Fabricius)(向日葵甲);来自瓢甲科(Coccinellidae)的甲虫,包括、但不限于:墨西哥豆瓢虫(Epilachna varivestis Mulsant)(Mexican bean beetle);来自金龟科(Scarabaeidae)的金龟子和其他甲虫,包括、但不限于:牧草金龟(Antitrogus parvulusBritton)(Childers cane grub);北方圆头犀金龟(Cyclocephala borealis Arrow)(northern masked chafer,蛴螬);南方圆头犀金龟(C.immaculata Olivier)(southernmasked chafer,蛴螬);白毛革鳞鳃金龟(Dermolepida albohirtum Waterhouse)(Greyback cane beetle);甘蔗犀金龟(Euetheola humilis rugiceps LeConte)(sugarcane beetle);Lepidiota ffenchi Blackburn(French’s cane grub);胡萝卜金龟(Tomarus gibbosus De Geer)(carrot beetle);T.subtropicus Blatchley(sugarcanegrub);Phyllophaga crinita Burmeister(蛴螬);P.latifrons LeConte(June beetle);日本金龟(Popillia japonica)(Japanese beetle);欧洲金龟(Rhizotrogus majalisRazoumowsky)(European chafer);来自皮蠹科(Dermestidae)的地毯圆皮蠹(carpetbeetle);来自叩甲科(Elateridae)的铁线虫(wireworm)、伪金针虫属(Eleodes)物种、梳爪叩甲属(Melanotus)物种,包括M.communis Gyllenhal(铁线虫);单叶叩甲属(Conoderus)物种;Limonius属物种;锥尾叩甲属(Agriotes)物种;Ctenicera属物种;Aeolus属物种;来自小蠹科(Scolytidae)的小蠹(bark beetle);来自拟步甲科(Tenebrionidae)的甲虫;来自天牛科(Cerambycidae)科的甲虫,例如、但不限于Migdolus fryanus Westwood(longhorn beetle);和来自吉丁虫科(Buprestidae)的甲虫,包括、但不限于Aphanisticuscochinchinae seminulum Obenberger(leaf-mining buprestid beetle)。
翅目(Diptera)的成体和未成熟体是值得关注的,包括潜叶虫美洲黍潜叶蝇(Agromyza parvicornis Loew)(玉米斑潜叶蝇(corn blotch leafminer));摇蚊,包括、但不限于:高粱瘿蚊(Contarinia sorghicola Coquillett(高粱摇蚊(sorghum midge));黑森瘿蚊(Mayetiola destructor Say)(黑森蝇(Hessian fly));向日葵籽摇蚊(Neolasioptera murtfeldtiana Felt)(sunflower seed midge));麦红吸浆虫(Sitodiplosis mosellana Géhin)(小麦摇蚊);果实蝇(实蝇科(Tephritidae))、黑麦秆蝇(Oscinella frit Linnaeus)(瑞典秆蝇(frit flies));蛆,包括、但不限于:地种蝇属(Delia)物种,包括灰地种蝇(Delia platura Meigen)(seedcorn maggot);麦种蝇(D.coarctata Fallen)(wheat bulb fly);夏厕蝇(Fannia canicularis Linnaeus)、小舍蝇(F.femoralis Stein)(lesser house flies);美洲麦杆蝇(Meromyza americanaFitch)(wheat stem maggot);舍蝇(Musca domestica Linnaeus)(家蝇类);厩螫蝇(Stomoxys calcitrans Linnaeus)(厩蝇类);秋家蝇、角蝇、丽蝇,金蝇属(Chrysomya)物种;伏蝇属(Phormia)物种;和其他蝇类(muscoid fly)害虫、马蝇类虻属(Tabanus)物种;肤蝇类马胃蝇属(Gastrophilus)物种;狂蝇属(Oestrus)物种;牛蝇类牛皮蝇属(Hypoderma)物种;鹿蝇类斑虻属(Chrysops)物种;绵羊虱蝇(Melophagus ovinus Linnaeus)(虱蝇类);和其他的短角亚目(Brachycera)、蚊类伊蚊属(Aedes)物种;按蚊属(Anopheles)物种;库蚊属(Culex)物种;黑蝇类原蚋属(Prosimulium)物种;蚋属(Simulium)物种;拟蚊蠓、白蛉、眼蕈蚊(sciarid)和其他长角亚目(Nematocera)。
作为目的昆虫,包括半翅目的那些昆虫,例如、但不限于以下科:球蚜科(Adelgidae)、粉虱科(Aleyrodidae)、蚜科(Aphididae)、链蚧科(Asterolecaniidae)、沫蝉科(Cercopidae)、叶蝉科(Cicadellidae)、蝉科(Cicadidae)、菱飞虱科(Cixiidae)、软介壳虫科(Coccidae)、缘蝽科(Coreidae)、胭蚧科(Dactylopiidae)、飞虱科(Delphacidae)、盾蚧科(Diaspididae)、毡蚧科(Eriococcidae)、蛾蜡蝉科(Flatidae)、蜡蝉科(Fulgoridae)、圆飞虱科(Issidae)、长蝽科(Lygaeidae)、硕蚧科(Margarodidae)、角蝉科(Membracidae)、盲蝽科(Miridae)、旌介壳虫科(Ortheziidae)、蝽科(Pentatomidae)、刺葵介壳虫科(Phoenicococcidae)、根瘤蚜科(Phylloxeridae)、粉蚧科(Pseudococcidae)、木虱科(Psyllidae)、红蝽科(Pyrrhocoridae)和网蝽科(Tingidae)。
来自半翅目的农艺学上重要的成员包括、但不限于:喜绿蝽(Acrosternum hilareSay)(green stink bug);豌豆蚜(Acyrthisiphon pisum Harris)(pea aphid);球蚜属(Adelges spp.)物种(球蚜类);苜蓿褐盲蝽(Adelphocoris rapidus Say)(rapid plantbug);南瓜缘蝽(Anasa tristis De Geer)(squash bug);花生蚜(Aphis craccivoraKoch)(cowpea aphid);黑豆蚜(A.fabae Scopoli)(black bean aphid);棉蚜(A.gossypiiGlover)(cotton aphid,melon aphid);玉米根蚜(A.maidiradicis Forbes)(corn rootaphid);苹果黄蚜(A.pomi De Geer)(apple aphid);绣线菊蚜(A.spiraecola Patch)(spirea aphid);印尼轮盾介壳虫(Aulacaspis tegalensis Zehntner)(sugarcanescale);茄沟无网蚜(Aulacorthum solani Kaltenbach)(foxglove aphid);烟粉虱(Bemisia tabaci Gennadius)(tobacco whitefly,sweetpotato whitefly);银叶粉虱(B.argentifolii Bellows&Perring)(silverleaf whitefly);美洲谷长蝽(Blissusleucopterus leucopterus Say)(chinch bug);负子蝽科(Blostomatidae)物种;甘蓝短棒蚜(Brevicoryne brassicae Linnaeus)(cabbage aphid);梨木虱(Cacopsylla pyricolaFoerster)(pear psylla);马铃薯衣壳蝽(Calocoris norvegicus Gmelin)(potatocapsid bug);草莓蚜(Chaetosiphon fragaefolii Cockerell)(strawberry aphid);臭虫科(Cimicidae)物种;缘蝽科(Coreidae)物种;方翅网蝽(Corythuca gossypii Fabricius)(cotton lace bug);番茄蝽(Cyrtopeltis modesta Distant)(tomato bug);烟草小盲蝽(C.notatus Distant)(suckfly);沫蝉(Deois flavopicta)(spittlebug);柑橘粉虱(Dialeurodes citri Ashmead)(citrus whitefly);皂荚蝽(Diaphnocoris chlorionisSay)(honeylocust plant bug);麦双尾蚜(Diuraphis noxia Kurdjumov/Mordvilko)(俄罗斯小麦蚜(Russian wheat aphid));竹禾盾介壳虫(Duplachionaspis divergensGreen)(armored scale);玫瑰苹果蚜(Dysaphis plantaginea Paaserini)(rosy appleaphid);棉蝽(Dysdercus suturellus)(cotton stainer);甘蔗灰粉蚧(Dysmicoccus boninsis Kuwana)(gray sugarcane mealybug);蚕豆微叶蝉(Empoascafabae Harris)(potato leafhopper);苹果绵蚜(Eriosoma lanigerum Hausmann)(woollyapple aphid);Erythroneoura科物种(葡萄斑叶蝉);港岛甘蔗飞虱(Eumetopina flavipesMuir)(Island sugarcane planthopper);扁盾蝽属(Eurygaster)物种;褐臭椿(Euschistus servus Say)(brown stink bug);一斑臭蝽(E.variolarius Palisot deBeauvois)(one-spotted stink bug);长蝽属(Graptostethus)物种(长蝽系群(complexof seed bugs));和桃大尾蚜Hyalopterus pruni Geoffroy)(mealy plum aphid);吹绵蚧(Icerya purchasi Maskell)(cottony cushion scale);洋葱蝽(Labopidicola alliiKnight)(onion plant bug);灰飞虱(Laodelphax striatellus Fallen)(smaller brownplanthopper);松叶根蝽(Leptoglossus corculus Say)(leaf-footed pine seed bug);Leptodictyatabida Herrich-Schaeffer(sugarcane lace bug);萝卜蚜(Lipaphiserysimi Kaltenbach)(turnip aphid);长绿盲蝽(Lygocoris pabulinus Linnaeus)(common green capsid);牧草盲蝽(Lygus lineolaris Palisot de Beauvois)(tarnished plant bug);豆荚草盲蝽(L.Hesperus Knight)(Western tarnished plantbug);牧草盲蝽(L.pratensis Linnaeus)(common meadow bug);长毛草盲蝽(L.rugulipennis Poppius)(European tarnished plant bug);马铃薯长管蚜(Macrosiphum euphorbiae Thomas)(potato aphid);翠菊叶蝉(Macrostelesquadrilineatus Forbes)(aster leafhopper);周期蝉(Magicicada septendecimLinnaeus)(periodical cicada);甘蔗沫蝉(Mahanarvafimbriolata)(sugarcanespittlebug);甘蔗小蝉(Mposticata)(little cicada of sugarcane);高粱蚜(Melanaphis sacchari Zehntner)(sugarcane aphid);粉蚧(Melanaspis glomerataGreen)(black scale);麦无网长管蚜(Metopolophium dirhodum Walker)(rose grainaphid);烟蚜(Myzus persicae Sulzer)(peach-potato aphid,green peach aphid);莴苣蚜(Nasonovia ribisnigri Mosley)(lettuce aphid);黑尾叶蝉(Nephotettix cinticepsUhler)(green leafhopper);二条黑尾叶蝉(N.n igropictus)(rice leafhopper);稻绿蝽Nezara viridula Linnaeus)(southern green stink bug);褐飞虱(Nilaparvatalugens)(brown planthopper);拟麦长蝽(Nysius ericae Schilling)(false chinchbug)茶黄蓟马(Nysius raphanus Howard)(假麦长蝽);稻蝽(Oebalus pugnax Fabricius)(rice stink bug);大马利筋长蝽(Oncopeltus fasciatus Dallas)(large milkweedbug);Orthops campestris Linnaeus;瘿绵蚜属(Pemphigus)物种(根蚜和瘿蚜);玉米飞虱(Peregrmus maidis Ashmead)(corn planthopper);甘蔗扁角飞虱(Perkinsiellasaccharicida Kirkaldy)(sugarcane delphacid);长山核桃根瘤蚜(Phylloxeradevastatrix Pergande)(pecan phylloxera);桔臀纹粉蚧(Planococcus citri Risso)(citrus mealybug);苹盲蝽(Plesiocoris rugicollis Fallen)(apple capsid);四线盲蝽(Poecilocapsus lineatus Fabricius)(four-lined plant bug);棉盲蝽(Pseudatomoscelis seriatus Reuter)(cotton fleahopper);粉蚧属(Pseudococcus)物种(其他的粉蚧系群);Pulvinaria elongata Newstead(cottony grass scale);蔗短足蜡蝉(Pyrilla perpusilla Walker)(sugarcane leafhopper);红蝽科(Pyrrhocoridae)物种梨园蚧(Quadraspidiotus perniciosusComstock)(San Jose scale);猎蝽科(Reduviidae)物种;玉米缢管蚜(Rhopalosiphum maidis Fitch)(corn leaf aphid);禾谷缢管蚜(R.padi Linnaeus)(bird cherry-oat aphid);甘蔗红粉蚧(Saccharicoccussacchari Cockerell)(pink sugarcane mealybug);Scaptocoris castanea Perty(brownroot stink bug);麦二叉蚜(Schizaphis graminum Rondani)(greenbug);Sipha flavaForbes(yellow sugarcane aphid);麦长管蚜(Sitobion avenae Fabricius)(Englishgrain aphid);白背飞虱(Sogatella furcifera Horvath)(white-backed planthopper);稻条背飞虱(Sogatodes oryzicola Muir)(rice delphacid);白斑盲蝽(Spanagonicusalbofasciatus Reuter)(whitemarked fleahopper);斑点苜蓿蚜(Therioaphis maculataBuckton)(spotted alfalfa aphid);谷蛾科(Tinidae)物种;桔二叉蚜(Toxopteraaurantii Boyer de Fonscolombe)(black citrus aphid);和褐色橘蚜(T.citricidaKirkaldy)(brown citrus aphid);纹翅粉虱(Trialeurodes abutiloneus)(bandedwingedwhitefly)和温室白粉虱(T.vaporariorum Westwood)(greenhouse whitefly);柿木虱(Trioza diospyri Ashmead)(persimmon psylla);和苹果白叶蝉(Typhlocyba pomariaMcAtee)(white apple leafhopper)。
另外,包括蜱螨目(Acari)(螨类)的成体和幼虫,例如小麦瘤瘿螨(Aceriatosichella Keifer)(小麦卷叶螨(wheat curl mite))苹果全爪螨(Panonychus ulmiKoch)(欧洲红螨(European red mite));麦岩螨(Petrobia latens Müller)(褐色小麦螨(brown wheat mite));班氏细蟎(Steneotarsonemus bancrofti Michael)(甘蔗细蟎(sugarcane stalk mite));叶螨科(Tetranychidae)的叶螨和红螨、Oligonychus grypusBaker&Pritchard、甘蔗叶螨(O.indicus Hirst)(sugarcane leaf mite)、草地小爪螨(O.pratensis Banks)(Banks grass mite)、O.stickneyi McGregor(sugarcane spidermite);二斑叶螨(Tetranychus urticae Koch)(two spotted spider mite);迈叶螨(T.mcdanieli McGregor)(McDaniel mite);朱砂叶螨(T.cinnabarinus Boisduval)(carmine spider mite);土耳其斯坦叶螨(T.turkestani Ugarov&Nikolski)(strawberryspider mite)、细须螨科(Tenuipalpidae)的扁平螨类、葡萄短须螨(Brevipalpus lewisiMcGregor)(citrus flat mite);瘿螨科(Eriophyidae)中的锈螨和芽瘿螨以及其他食叶螨和对人类和动物健康重要的螨,即表皮螨科(Epidermoptidae)的尘螨、蠕形螨科(Demodicidae)的毛囊螨、食甜螨科(Glycyphagidae)的谷螨,硬蜱科(Ixodidae)的蜱类。黑脚硬蜱(Ixodes scapularis Say)(鹿蜱(deer tick));全环硬蜱(I.holocyclus Neumann)(澳洲寄生蜱(Australian paralysis tick));变异革蜱(Dermacentor variabilis Say)(美洲犬蜱(American dog tick));美洲钝眼蜱(Amblyomma americanum Linnaeus)(lonestar tick);以及痒螨科(Psoroptidae)、蒲螨科(Pyemotidae)和疥螨科(Sarcoptidae)的痒螨和疥螨。
缨尾目(Thysanura)的昆虫害虫是值得关注的,例如衣鱼(Lepisma saccharinaLinnaeus)(silverfish);家衣鱼(Thermobia domestica Packard)(小灶衣鱼(firebrat))。
所涵盖的其他节肢害虫包括:蜘蛛目中的蜘蛛,例如棕色遁蛛(Loxoscelesreclusa Gertsch&Mulaik(brown recluse spider);和红斑寇蛛(Latrodectus mactansFabricius)(黑寡妇蜘蛛(black widow spider));和蚰蜓目(Scutigeromorpha)的多足类例如蚰蜒(Scutigera coleoptrata Linnaeus)(house centipede)。此外,等翅目(Isoptera)中的病害昆虫是值得关注的,包括白蚁科(termitidae)的那些病害昆虫,例如、但不限于白蚁(Cornitermes cumulans Kollar)、Cylindrotermes nordenskioeldiHolmgren和Pseudacanthotermes militaris Hagen(甘蔗白蚁);以及鼻白蚁科(Rhinotermitidae)的那些病害昆虫,包括、但不限于Heterotermes tenuis Hagen。缨翅目(Thysanoptera)的昆虫也是值得关注的,包括、但不限于蓟马类,例如微直鬃蓟马(Stenchaetothrips minutus van Deventer)(甘蔗蓟马(sugarcane thrip))。
本发明的实施方式在以下实施例中进一步界定。应当理解这些实施例仅以示例的方式给出。由以上讨论和这些实施例,本领域技术人员可以确定本发明的核心特征,并在不背离本发明的精神和范围的情况下,可对本发明的实施方式作出各种变通和修改以使之适应各种用途和条件。因此,除了本文显示和描述的那些实施方式之外,本发明实施方式的各种修改将因前面的描述而对本领域技术人员变得显而易见。这类修改也当视为落入所附权利要求的范围内。
本文给出的每个参考文献的公开内容以引用方式整体并入本文。
实施例
实施例1通过农杆菌转化法转化玉米并且再生含有Cry1F、Cry34Ab1、Cry35Ab1
(Cry34/35Ab1)和Pat基因的转基因植物
4114玉米由农杆菌介导的质粒PHP27118转化产生。该事件含有cry1F、cry34Ab1、cry35Ab1和pat基因盒,它们赋予针对某些鳞翅类和鞘翅类害虫的抗性。
具体而言,第一盒含有截短形式的来自Bt鲇泽变种的cry1F基因。cry1F基因的插入赋予针对鳞翅目害虫(包括ECB和FAW)损害的抗性。Cry1F蛋白(SEQ ID NO:1)由605个氨基酸组成并且具有大约68kDa的分子量。cry1F基因的表达受玉米多聚遍在蛋白启动子控制(Christensen等人1992,上文),从而提供Cry1Fy1F蛋白在玉米中的组成型表达。该区域还包括与天然多聚遍在蛋白启动子相连的5′UTR和内含子。cry1Fy1F基因的终止子是来自根癌农杆菌(A.tumefaciens)Ti质粒pTi15955的开放读框25(ORF 25)中的聚(A)添加信号(Barker等人,1983,上文)。
第二盒含有从Bt菌株PS149BS149B1分离而得的cry34Ab1基因(美国专利No.6,127,180;No.6,624,145和No.6,340,593)。Cry34Ab1蛋白(SEQ ID NO:2)的长度是123个氨基酸并且具有大约14kDa的分子量。cry34Ab1基因的表达受带有5′UTR和内含子的玉米多聚遍在蛋白启动子的第二副本控制(Christensen等人,1992,上文)。cry34Ab1基因的终止子是pinII终止子(Keil等人,1986,上文;An等人,1989,上文)。
第三基因盒含有cry35Ab1基因,其也从Bt菌株PS149BS149B1分离而来(美国专利No.6,083,499;No.6,548,291和No.6,340,593)。Cry35Ab1蛋白(SEQ ID NO:3)具有383个氨基酸的长度和大约44kDa的分子量。Cry34Ab1和Cry35Ab1蛋白在植物中的同时表达赋予鞘翅类昆虫(包括WCRW)抗性。Cry35Ab1基因的表达受普通小麦(小麦)过氧化物酶启动子和前导序列控制(Hertig等人,上文)。Cry35Ab1基因的终止子是pinII终止子的第二副本(Keil等人,1986,上文;An等人,1989,上文)。
第四也即最后一个基因盒含有来自绿色产色链霉菌的pat的已经为玉米中表达进行优化的形式。pat基因表达PAT,其赋予膦丝菌素(草铵膦-铵)耐受性。PAT蛋白(SEQ IDNO:4)的长度是183个氨基酸并且具有大约21kDa的分子量。pat基因的表达受来自CaMV 35S转录物的启动子区和终止子区控制(Franck等人,1980,上文;Odell等人,1985,上文;Pietrzak,等人,1986,上文)。还提供了含有所述DNA构建体的植物。表1中进一步描述(SEQID NO:5中所述的)PHP27118 T-DNA中的遗传元件及其来源。
表1:质粒PHP27118的T-DNA区域中的基因元件
将玉米(玉蜀黍)的未成熟胚从授粉后9至11天正在发育的颖果中取出并且用含有质粒PHP27118的根癌农杆菌菌株LBA4404接种(图1),基本上如Zhao(美国专利No.5,981,840,所述专利的内容在此通过引用方式并入)中所述那样。图2中显示PHP27118的T-DNA区。在胚和农杆菌于没有选择剂的固体培养基上共培育三至六天后,将胚转移至没有除草剂选择但含有羧苄青霉素的培养基。在这种培养基上三至五天后,将胚转移至刺激玉米体细胞性胚发生并含有双丙胺膦以选择表达pat转基因的细胞的选择培养基。该培养基也含有羧苄青霉素以杀死任何剩余的农杆菌。在这种选择培养基上六至八周后,鉴定展示抗双丙胺膦性的正在生长的健康愈伤组织。随后将推定性转基因愈伤组织再生以产生T0小植物。
从T0小植物取得样品用于PCR分析以验证插入的cry1F、cry35Ab1、cry34Ab1和/或pat基因的存在和拷贝数。证实玉米事件DP-004114-3含有单拷贝的T-DNA(见实施例2和3)。除这个分析之外,还通过PCR对T0小植物分析某些农杆菌双元载体骨架序列的存在(数据未显示)。选出确定具有单拷贝插入的基因并且农杆菌骨架序列为阴性的植物用于进一步温室繁殖。通过实施众多生物检验法,对这些选出的T0植物筛选性状功效和蛋白质表达(见实施例5)。将符合全部标准的T0植物推进并与近交系杂交以产生种子用于进一步测试。图3中呈现转化和事件发展的示意性概述。
实施例2.玉米事件DP-004114-3的鉴定
从来自4114玉米的试验种子和对照物质(来自非基因修饰玉米的种子,所述玉米具有代表事件背景的遗传背景)的叶组织分离出基因组DNA并且使用构建体特异性引物对,使该基因组DNA进行定量PCR扩增。PCR产物在琼脂糖凝胶上分离以证实从试验种子分离的基因组DNA中存在插入的构建体,和从对照种子分离的基因组DNA中不存在插入的构建体。参比标准物(低DNA质量梯;Invitrogen Corporation目录号10380-012)用来确定PCR产物大小。通过重复实验三次评估构建体特异性PCR方法的可靠性。通过来自4114玉米的基因组DNA的多个稀释物评价PCR扩增的灵敏度。
试验和对照叶样品(V5-V7叶期)从DuPont实验站(Wilmington,DE)处自PioneerHi-Bred(Johnston,IA)获得的种子所培育的植物收获。使用标准脲提取方案从试验和对照叶组织进行基因组DNA提取。
使用NanoDrop 1000分光光度计,使用ND-1000V3.6软件(ThermoScientific,Wilmington,DE)和Quant-iT试剂(Invitrogen,Carslbad,CA),对基因组DNA定量。DNA样品在琼脂糖凝胶上可视化以证实定量值并且确定DNA质量。
从4114玉米的叶组织分离的基因组DNA样品和对照样品接受使用构建体特异性引物对(SEQ ID NO:7和SEQ ID NO:8)的PCR扩增(Roche高保真PCR主试剂盒,Roche目录号12140314001),其中所述构建体特异性引物对涵盖玉米ORF 25终止子和遍在蛋白启动子(见图2),并且允许唯一鉴定4114玉米中插入的T-DNA。第二引物组(SEQ ID NO:9和SEQ IDNO:10)用来扩增内源玉米转化酶基因(GenBank登录号AF171874.1)作为PCR扩增的阳性对照。表2给出了每个引物组的PCR靶位点和预期PCR产物的大小。表3给出了PCR试剂和反应条件。在本研究中,全部PCR反应中均使用50ng叶基因组DNA。
表2:PCR基因组DNA靶位点和PCR产物的预期大小
表3:PCR试剂和反应条件
ddH2O:双蒸水
*Roche高保真主混合物
在使用质粒PHP27118(10ng)作为模板和全部4114玉米DNA样品的PCR反应中观察到由构建体特异性引物组(SEQ ID NO:7和SEQ ID NO:8)扩增的大小约300bp的PCR产物,但是在全部对照玉米样品和无模板对照中不存在所述PCR产物。该实验重复3次,并且获得相似的结果。对来自五个4114玉米植物和五个对照玉米植物的DNA提取物所观察到的结果与对含有4114玉米基因组DNA样品所预期的PCR产物大小(287bp)非常一致。采用检测内源玉米转化酶基因的引物组(SEQ ID NO:9和SEQ ID NO:10)的PCR反应后,对4114玉米样品和对照玉米样品均观察到大小约220bp的PCR产物。这些结果与对含有玉米内源转化酶基因的基因组DNA样品所预期的PCR产物大小(225bp)非常一致。在无模板对照中未观察到内源靶条带。
为了评估PCR扩增的灵敏度,将多个浓度的来自4114玉米的单一DNA样品稀释于非基因修饰对照DNA中,从而产生范围从500fg、5pg、10pg、50pg、100pg、500pg、5ng和50ng(基因组DNA在全部PCR样品中的总量是50ng)的4114玉米DNA量。每个稀释物如先前实施那样进行PCR扩增。基于这种分析,确定检测限(LOD)是在50ng总DNA中大约100pg的4114玉米DNA,或0.2%的4114玉米DNA。
总之,使用4114玉米的构建体特异性引物组的定性PCR分析证实,试验植物含有自质粒PHP27118的插入的T-DNA,如因构建体特异性靶条带在分析的全部试验植物样品中存在并且在非基因修饰对照植物中不存在而显而易见。这个结果是可重现的。试验植物和对照植物均含有内源玉米转化酶基因。本分析在所述条件下的灵敏度是在50ng总基因组DNA中大约100pg的4114玉米基因组DNA,或0.2%的4114玉米基因组DNA。
实施例3用于完整性和拷贝数的DP-004114-3玉米的DNA印迹分析
DNA印迹分析用来证实来自PHP27118的插入的T-DNA的完整性和拷贝数并且证实在4114玉米中存在cry1F、cry34Ab1、cry35Ab1和pat基因盒。
选择来自4114玉米T1代的五个单独植物用于DNA印迹分析。从4114玉米(试验)和非转基因玉米(对照)植物收获幼叶材料并且立即置于干冰上。将冷冻的样品冻干,并且使用CTAB提取方法从试验组织和对照组织提取基因组DNA。
在如下文详述的限制性酶消化后,DNA片段在琼脂糖凝胶上分离、脱嘌呤化、变性和原位中和,并且使用如对TURBOBLOTTERTM Rapid Downward Transfer System(Schleicher#Schuell)所描述的方法,转移至20x SSC缓冲液中的尼龙膜。转移至所述膜后,通过紫外线交联使DNA与该膜结合。
完整性
因为存在三个位于T-DNA内部的Hind III位点,所以选择限制性酶Hind III用于完整性的DNA印迹分析(图2)。大约1-3μg基因组DNA用Hind III消化和在琼脂糖凝胶上按大小分离。作为阳性对照,将大约15pg含有PHP27118 T-DNA的质粒掺入对照植物DNA样品,消化和装载在琼脂糖凝胶上。还包括阴性对照以验证探针与玉米基因组的背景杂交。
与PHP27118T-DNA(基因元件参见图2)上的cry1F、cry34Ab1、cry35Ab1和pat基因同源的四个探针用于杂交以证实所述基因的存在。为了产生这些探针,将同源于cry1F、cry34Ab1、Cry35Ab1和pat基因的片段通过PCR从含有PHP27118 T-DNA的质粒中产生,在琼脂糖凝胶上按大小分离,并且使用凝胶提取试剂盒(Qiagen)纯化。随后使用以[32p]dCTP进行随机引发标记的RediprimeTM II DNA标记系统(Amersham),从所述片段产生全部DNA探针。
基本上如制造商(Stratagene)描述,使用杂交溶液,将标记的探针与尼龙膜上的靶DNA杂交以检测特定片段。在高严格性下实施杂交后的洗涤。印迹物在-80℃下对X-射线胶片曝光一个或多个时间点以检测杂交片段。
因为Hind III酶位点已知在T-DNA内部,所以测定每个探针的确切预期条带大小(表4,图2)。对于T-DNA的完整副本,cry1F探针预期与3891bp的片段杂交。cry34Ab1、cry35Ab1和pat基因探针预期与7769bp的片段杂交。来自下述试样的片段将证实插入的T-DNA的完整性和每个基因的存在,所述试样的片段匹配预期的大小以及匹配质粒对照样品中的条带。
采用Hind III和cry1F、cry34Ab1、cry35Ab1和pat基因探针的DNA印迹分析的结果证实了预期片段大小,并从而证实所述T-DNA完整插入每个事件中并且所述基因的每一个均存在。
随cry1F探针观察到一条大约4kb的条带,其与预期片段大小一致。在质粒阳性对照泳道中观察到一条大约4kb的相似条带,假定该条带是3891bp的预期条带。基于所述事件中的杂交条带相对于质粒阳性对照中条带的相等迁移率,证实T-DNA的含有cry1F的部分已经完整插入4114玉米中。
在采用cry34Ab1探针的杂交中,在事件中并且还在质粒阳性对照中观察到一条大约8kb的条带。在质粒阳性对照泳道中的杂交条带假定是7769bp的预期条带。因为事件中的杂交条带已经相对于该条带同等地迁移,所以证实T-DNA的含有cry34Ab1的这个部分完整地插入。
类似地,采用cry35Ab1和pat的杂交如预期那样与植物和质粒阳性对照中的相同7769bp片段杂交。这些结果证实,T-DNA的含有cry35Ab1和pat基因的部分已经完整插入。
这个DNA印迹分析证实,4114玉米含有来自PHP27118的T-DNA的完整副本,其含有cry1F、cry34Ab1、Cry35Ab1和pat基因。
表4:对于用Hind III消化4114玉米DNA所预期和在DNA印迹上观察到的杂交片段 的总结
1基于PHP27118 T-DNA图谱的预期片段大小(图2)。
2全部观察到的片段相对于质粒阳性对照同等地迁移,并且因此证实代表PHP27118 T-DNA完整部分。
拷贝数
cry1F和pat探针在DNA印迹杂交中用来评价4114玉米中插入物的拷贝数。
因为存在位于T-DNA内部的单个Bcl I位点,所以选择限制性酶Bcl I用于拷贝数的DNA印迹分析(图2)。将大约3μg来自事件4114的T1代各个植物的基因组DNA用Bcl I消化并且在琼脂糖凝胶上按大小分离。将含有PHP27118 T-DNA的质粒掺入对照植物DNA样品,消化和装载在琼脂糖凝胶上以充当阳性杂交对照。还包括阴性玉米DNA对照以验证探针与玉米基因组的背景杂交。在Bcl I印迹物上包括地高辛(DIG)标记的DNA分子量标记VII(Roche,Indianapolis,IN)作为杂交片段的大小标准。
cry1F和pat基因的探针也通过掺入地高辛(DIG)标记的核苷酸[DIG-11]-dUTP至其片段中的PCR反应进行标记。根据在PCR DIG探针合成试剂盒(Roche)中提供的方法,实施分离片段的PCR标记。
将DIG-标记的探针与4114事件的T1代的Bcl I DNA印迹物杂交。基本上如制造商所述,使用DIG Easy Hyb溶液(Roche),将探针与靶DNA杂交以检测特定片段。在高严格性下实施杂交后洗涤。使用CDP-Star化学发光核酸检测系统(Roche)检测与结合的片段杂交的DIG标记探针。印迹物在室温下对X-射线胶片曝光一个或多个时间点以检测杂交片段。按照制造商推荐,从膜剥离杂交的探针,随后用另外的探针杂交。
选择在T-DNA内部具有单一限制性位点(图2)的限制性酶Bcl I来证实4114玉米中单个PHP27118 T-DNA插入物的存在。Bcl I的位点位于T-DNA的bp 2546处(图2)并且将对单一插入的T-DNA产生大于约2500bp和9400bp的片段。采用pat探针的杂交将根据杂交条带的数目(例如,一条杂交条带指示该元件的一个副本)指示在所述事件中存在的该元件的副本数。pat探针将与大于9400bp的片段杂交。因为Bcl I限制性酶位点在cry1F基因内部,所以预期cry1Fy1F探针与两个片段杂交并且对单一T-DNA插入产生两个条带(图2)。
表5中总结对4114玉米采用Bcl I以及cry1F和pat基因探针的DNA印迹分析的结果。
表5:对于4114玉米的Bcl I消化物所预期的和在DNA印迹上观察到的杂交片段的 总结。
1基于PHP27118 T-DNA图谱的预期片段大小(图2)。
2基于DIGVII分子量标记的迁移率,近似得到的全部观察到的片段大小。
3因cry1F基因内部的Bcl I限制性位点,故采用cry1F探针时预期有两个片段。
采用Bcl I消化和cry1F探针的4114玉米DNA印迹分析的结果如预期那样显示两个条带,大于8.6kb的一个条带和大约3.1kb的第二个条带。因cry1F基因内部Bcl I位点的位置,故对于单个插入物,预期有两个条带,因此这些结果表明在4114玉米中存在cry1F的单拷贝。采用Bcl I消化和pat探针的4114玉米DNA印迹分析的结果如预期那样显示大于8.6kb的单一条带,其匹配较大cry1F条带的大小。这些结果表明在玉米事件4114中还存在pat基因的单一插入。
由于cry34Ab1和cry35Ab1基因位于与pat基因和部分cry1F基因相同的片段上,且处于T-DNA上cry1F和pat基因之间,按照推论,这也表明该事件很可能含有这些基因中每一者的单拷贝。
实施例4玉米事件DP-004114-3的插入物和基因组边界区的测序表征
测定插入物和基因组边界区的序列以证实插入的DNA的完整性并表征在4114玉米中存在的插入位点侧翼的基因组序列。总计,证实了16,752bp的4114玉米基因组序列,其包含2,422bp的5′基因组边界序列、2,405bp的3′基因组边界序列、和11,925bp来自PHP27118的插入的T-DNA。发现4114玉米中的插入的T-DNA具有在右边界(RB)末端上的29bp缺失和在左边界(LB)末端上的24bp缺失。全部剩余序列是完整的并且与质粒PHP27118的序列相同。通过PCR扩增和将源自4114玉米和对照玉米植物的基因组边界区测序,证实4114玉米的5′和3′基因组边界区是玉米源的。
含有事件DP-004114-3的种子从4114玉米的T1ST1S2代获得。对照种子从具有与4114玉米相似的遗传背景但不含cry1F、cry34Ab1、cry35Ab1和pat基因盒的玉米系获得。全部种子均从Pioneer Hi-Bred International,Inc.(Johnston,IA)获得。低分子量DNA梯(Invitrogen Corp.,Carlsbad,CA)和高分子量DNA梯(Invitrogen Corp.)用于凝胶电泳以估计琼脂糖凝胶上的DNA片段大小。
在DuPont实验站(Wilmington,DE)的生长室中播种4114玉米种子和对照种子以产生用于本项研究的植物组织。每钵播种一粒种子,并且将该钵唯一地标识。全部植物以调节用于健康植物生长的光、温度和水培育。从对照和4114玉米植物收集叶样品。对于每个单独植物,将叶材料收集于预标记的袋内,置于干冰上并且在收集后转移至超低温冰箱(<<<<-55℃)。全部样品维持冷冻直至组织处理。
通过事件特异性PCR分析证实基因型
从全部试验植物和对照植物取得叶样品用于事件特异性PCR分析。使用Extract-N-AmpTM Plant PCR试剂盒,按照对实时PCR分析描述的方法(Sigma-Aldrich,St.Louis,MO),从每份叶样品提取DNA。
使用ABI序列检测系统(Applied Biosystems,Inc.,Foster City,CA),对每份样品进行实时PCR。将探针(Applied Biosystems,Inc.)和引物组(Integrated DNA Technologies,Coralville,IA)设计组合,以检测来自4114玉米的靶序列。此外,针对参比玉米内源基因的第二探针和引物组用来证实每个反应中存在可扩增的DNA。该分析由实时PCR确定定性阳性/阴性响应(call)组成。使用Universal PCR Master Mix.NoUNG(Applied Biosystems,Inc.),分析提取出来的DNA。
4114玉米的阳性或阴性确定基于事件特异性靶PCR的CT(阈值循环)与玉米内源参比靶的CT的比较。如果所述事件和内源PCR靶在CT阈值以上扩增,则将植物评定为对该事件呈阳性。如果内源靶扩增而事件靶不扩增,则将植物评定为阴性。如果对于特定样品,两种靶均不扩增,则确定该样品是质量低劣的DNA样品或测试失败,并且重复分析。
全部4114玉米植物均对事件特异性PCR和PAT、Cry1F和Cry34Ab1蛋白呈阳性,而全部对照玉米植物均为阴性。表6中汇总结果。
表6. 4114玉米植物和对照玉米植物中事件特异性PCR分析及Cry1F、Cry34Ab1、和 PAT蛋白表达的总结
1.4114玉米的事件特异性实时PCR测定的总结。阳性(+)指示存在4114玉米事件。阴性(-)指示不存在4114玉米事件。
2.使用横向流装置时,4114玉米植物和对照玉米植物中Cry1F、Cry34Ab1和PAT蛋白表达的总结。阳性(+)指示存在所述蛋白质。阴性(-)指示不存在所述蛋白质。
DNA测序
将DNA片段克隆并且提交在Pioneer Crop Genetics Research测序设施(Wilmington,DE)测序。使用来自Gene Codes Corporation的SequencherTM软件(AnnArbor,Michigan)装配序列。使用Vector NTI 9.1.0(Invitrogen Corp),通过比较从4114玉米产生的T-DNA插入物序列与来自(用于转化以产生4114玉米的)质粒PHP27118的T-DNA区的序列,进行序列注释。
对用来产生4114玉米的质粒PHP27118的T-DNA区测序并且与4114玉米中的插入的T-DNA序列比较。
用了质粒PHP27118的T-DNA区的序列来设计引物对以表征4114玉米中的插入的T-DNA。使用来自四株不同的4114玉米植物的基因组DNA作为模板,产生六个重叠的PCR产物。将这些PCR产物克隆并测序。
5′和3′侧翼基因组边界区的测序。
使用几轮反向PCR(Silver和Keerikatte(1989)J.Virol.63:1924;Ochman等人,(1988)Genetics 120:621-623;Triglia等人,(1988)Nucl.Acids Res.16:8186)以锚定于插入的T-DNA的多个区域内部的引物,实施5′和3′侧翼基因组边界区的初步序列表征。从反向PCR获得的序列信息经过BLASTn分析并且显示与来自NCBI(国家生物技术信息中心)GenBank核苷酸数据库的玉米BAC克隆AC211214匹配。该序列随后用来设计跨越4114玉米中5′和3′插入物/基因组接点的引物。从四株4114玉米植物产生的PCR产物经克隆和测序以验证5′和3′插入物/基因组接点和基因组边界区。
此外,为了显示鉴定的5′和3′基因组边界区是玉米源的,对4114玉米植物和对照玉米植物在所述基因组区内部进行PCR。将每个PCR片段直接测序以验证其玉米源身份。
用了质粒PHP27118的T-DNA序列信息来设计引物以验证4114玉米中的插入序列(表7和8)。
表7.用来表征4114玉米中基因组边界区和插入的T-DNA的PCR引物
表8:用于PCR反应的引物的序列和位置
1在4114玉米序列中的位置。
碱基1-2,422=5′基因组边界区
碱基2,423-14,347=插入物
碱基14,348-16,752=3′基因组边界区。
为表征4114玉米中的插入的DNA,将PCR引物设计成以如表7中概述的六个独立、重叠的PCR产物:片段A至F(位置在图5中显示)以扩增T-DNA插入物。如预期那样,预测的PCR产物仅从4114玉米基因组DNA样品产生,并且不存在于对照玉米样品中。将这六个PCR产物克隆并测序。当比较4114玉米中的插入的T-DNA的序列与用来产生4114玉米的质粒PHP27118的T-DNA区时,确定RB末端上存在29bp缺失并且LB末端上存在24bp缺失。RB和LB末端缺失经常在农杆菌介导的转化中发生(Kim等人,(2007)Plant J. 51:779-791)。全部剩余序列是完整的并且与质粒PHP27118的序列相同。插入物的序列在SEQ ID NO:6中描述。
为验证额外的5′基因组边界序列,采用在5′基因组边界区中的正向引物(SEQ IDNO:11)和在插入的T-DNA内部的反向引物(SEQ ID NO:12)进行PCR。将所得到的来自4114玉米基因组DNA样品的2,511bp PCR片段A克隆并且测序(图3)。2,422bp的5′基因组边界区序列以SEQ ID NO:6的核苷酸1-2,422描述。
为验证额外的3′基因组边界序列,采用在插入的T-DNA内部的正向引物(SEQ IDNO:21)和在3′基因组边界区中的反向引物(SEQ ID NO:22)进行PCR。将所得到的来自4114玉米基因组DNA样品的2,612bp PCR片段F克隆并且测序(图3)。2,405bp的3′基因组边界区序列以SEQ ID NO:6的核苷酸14,348至16,752描述。
总计,证实了来自4114玉米基因组DNA的16,752bp序列:2,422bp的5′基因组边界序列、2,405bp的3′基因组边界序列和包含插入的T-DNA的11,925bp。
为了显示所鉴定的5′和3′侧翼基因组边界区是玉米源的,对4114玉米基因组DNA样品和对照玉米样品在所述5′和3′基因组边界区内部进行PCR(分别在SEQ ID NO:23和SEQID NO:24中描述的引物对以及在SEQ ID NO:25和SEQ ID NO:26中描述的引物对)。从4114玉米和对照玉米中均产生预期的PCR片段G(对于5′基因组区域的257bp)和PCR片段H(对于3′基因组区域的283bp)。将这些PCR产物克隆并测序,发现来自4114玉米和对照玉米的相应产物是相同的,因而证实所述序列是玉米基因组源的。
实施例5玉米事件DP-004114-3的昆虫功效
在4114玉米上产生的功效数据。田间试验比较了具有两种遗传背景的4114玉米与具有相同背景的阴性对照(等位基因系)。功效检验包括:在四个地点的第一代ECB(ECB1)叶损伤和第二代ECB(ECB2)茎秆损伤、在三个地点的WCRW根损伤和在一个地点的FAW叶损伤。在每个地点,单行样区以随机完全区组播种,三次重复,(20粒/样区x12个条目(entry)x3次重复=1实验/地点)。在出苗后对全部植物进行组织取样以通过事件特异性PCR证实事件的存在。剔除任何阴性者,并且将每一样区疏苗至目标株丛(stand):每样区10-15株均匀间隔的植物。
对于表征ECB1损伤的试验,将每株植物在大约1周内用大约100条ECB初生幼虫手工侵染3次(总计300条幼虫),在大约V5生长期开始。在最后成功侵染后大约三周,对每样区8株相邻植物(每种遗传背景、每个条目总计24株植物)进行叶损伤评级(基于9-1目视评级量表,其中9表示无损伤而1表示最大损伤),并且对每个处理计算平均值。表9中显示在4114玉米上的第一代ECB叶采食结果。
表9.DP-004114-3玉米抵抗第一代ECB幼虫的功效
a使用以下目视评级表对各个植物确定损伤评级:
9.无可见叶损伤或少数叶上的少量针状或细微穿孔型损伤。
8.少数叶上少量穿孔型病灶。
7.在若干叶上常见穿孔损伤。
6.若干叶具有穿孔和加长病灶(病灶长度<0.5″)。
5.若干叶具有加长病灶(病灶长度0.5″至1.0″)。
4.若干叶具有加长病灶(病灶长度>1.0″)。
3.在约一半叶上常见长病灶(>1.0″)。
2.在约三分之二叶上常见长病灶(>1.0″)。
1.大多数叶具有长病灶。
b在一个地点内,有相同字母的各平均值不具有显著性差异(Fisher受保护的LSD检验,P>0.05)。
对于表征ECB2损伤的试验,将上文用于ECB1侵染的相同植物稍后在生长季节在大约1周内再次用每株植物大约100条ECB初生幼虫手工侵染3次(总计300条幼虫),在R1生长期开始,此时大约50%的植物正扬播花粉。在最后一次侵染后大约50-60日,将每样区8株相邻植物(每种遗传背景、每个条目总计24株植物)的茎秆从原发穗(primary ear)上方的第四节间顶部至植物基部劈开。随后以厘米计测量ECB茎秆蛀道(ECBXCM)的总长度并且对每种植物记录。1cm或更短的蛀道视为入口孔(幼虫不能够在茎秆中建立)并且不包括在蛀道的总厘米数中。计算每个处理的平均值(蛀道的总厘米数)。表10中显示4114玉米的ECB2茎秆采食结果。
表10.DP-004114-3玉米抵抗第二代ECB幼虫的功效
b在一个地点内,带有相同字母的各平均值不具有显著性差异(Fisher受保护的LSD检验,P>0.05)。
还研究了WCRW造成的根损伤。对大约在V2生长期的植物用大约500粒WCRW卵手工侵染(总计~1,000粒卵/植物),办法是将所述WCRW卵施加在植物的每一侧的土壤中。另外,在具有WCRW天然侵染的高度可能性的田间样区播种。在大约R2生长期时评价植物的根部。将每样区五株相邻植物(每种遗传背景、每个条目总计45株植物)从样区移出并且用加压水冲洗。使用0-3的结节损伤评级表(node injury scale)对根部损伤评级(CRWNIS)(Oleson等人,(2005)J.Econ.Entomol.98(1):1-8),并计算每个处理的平均值。表11给出了因WCRW采食所致的平均根部损伤评级。
表11.DP-004114-3玉米抵抗WCR幼虫的功效
b使用0-3结节损伤评级表对各个植物根群确定损伤评级(Oleson等人2005,上文)。
c在某一地点内,带有相同字母的各平均值不具有显著性差异(Fisher受保护的LSD检验,P>0.05)。
对于FAW功效检验,将各株植物在大约V5生长期时用大约75条新生幼虫手工地侵染。在最后成功侵染后大约两周,对每样区8株连续植物(每种遗传背景、每个条目总计24株植物)评定叶的损伤(FAWLF基于9-1目视评级表,其中9表示无损伤,1表示最大损伤),并且对每个处理计算平均值。表12给出了表征FAW叶采食DP-004114-3的平均损伤评级。
表12.DP-004114-3玉米抵抗FAW幼虫的功效
a使用以下目视评级表对各个植物确定损伤评级:
9.无损伤至轮叶上存在针孔病灶。
8.轮叶上存在针孔和圆形小病灶
7.轮叶和缩卷叶上存在圆形小病灶和一些加长(矩形形状)小病灶,长度至多到0.5″。
6.一些轮叶和缩卷叶存在若干小的加长病灶,长度0.5″至1″。
5.一些叶和缩卷叶上存在若干加长的大病灶,长度大于1″′,和/或轮叶和缩卷叶中存在一些小尺寸至中等尺寸的均匀至不规则形状的孔(基底叶膜蚀透)。
4.若干轮叶和缩卷叶上存在若干加长的大病灶,和/或轮叶和缩卷叶中存在若干均匀至不规则形状的大孔。
3.若干轮叶上存在全部尺寸的许多加长病灶,外加轮叶和缩卷叶中存在若干均匀至不规则形状的大孔。
2.大部分轮叶和缩卷叶上存在全部尺寸的许多加长病灶,外加轮叶和缩卷叶中存在许多中等尺寸至大尺寸的均匀至不规则形状的孔。
1.轮叶和缩卷叶几乎完全被摧毁。
b在某一地点内,带有相同字母的各平均值不具有显著性差异(Fisher受保护的LSD检验,P>0.05)。
除了田间功效研究外,还以在实验室进行的亚致死性籽苗测定法(SSA)(美国公开No.2006/0104904,其内容在此通过引用方式并入)对4114玉米进行了评价。SSA法允许在没有大田环境混淆影响下比较4114玉米与未保护的对照(近等位基因系)的功效。SSA技术包括使WCRW初生幼虫群体暴露于含有所述4114玉米事件之一的玉米籽苗或非转基因(阴性对照)玉米籽苗。从卵初始孵育起,使幼虫暴露17天时间。SSA的实验装置是尺寸为23×30×10cm的单个塑料容器(Pactiv Corp.,Lake Forest,IL)。条目以随机完全区组设置,每个条目3次重复。对于每个条目,SSA的做法包括将115颗籽粒置于每个容器中,所述容器具有225mL的1%甲基硫菌灵杀菌剂溶液和1000mL的Metro-Mix 200植物生长培养基(Scotts-Sierra Horticultural Products Company,Marysville,OH)。在添加Metro-Mix后,立即将WCRW卵以每个容器1,000粒卵的比率侵染到每个容器的表面。WCRW卵在25℃下预孵育,以使得初始卵孵化正好在容器装好试样后5-7天发生。侵染的容器保存在具有如下设定的步入式环境室中:25℃,65%相对湿度和14∶10光照:黑暗循环。卵孵化后17天时,用布氏漏斗装置从所述容器中提取幼虫。从每个容器选择30条幼虫的随机子样本,并且在解剖显微镜下测量它们的头壳以将每条幼虫划归入3个龄期之一。收集的数据包括从处于三个潜在龄期中每一龄期的幼虫的数目所确定的幼虫群体的年龄结构。绘制了以图形方式显示每个条目的幼虫年龄分布的直方图并且如图4中所示那样目视比较。
表13提供了4114玉米的害虫谱。
表13.由表达Cry1F、Cry34Ab1和Cry35Ab1的DP-004114-3玉米控制或抑制的昆虫 害虫。
实施例6蛋白质表达和浓缩
植物材料的产生
来自PHNAR×BC3F3代的4114玉米在美国和加拿大的五个地点培育。每个地点采用含有四个区块的随机完全区组设计,其中每个区块由至少36英寸(0.9m)的缓冲距离隔开。每个条目在2行样区中播种,所述样区在每一侧邻接1行边界种子(border seed)。
叶组织收集和加工
在V9期在每个区块中收集一个叶组织样品。对于每个事件,从无偏选择、健康、代表性植物收集全部样品。通过选择已经从轮叶中出现至少8英寸(20cm,可见组织)的最年幼的叶,获得每个叶样品。如果该叶是受损或不健康的,则将其下方的下一片叶子采样。从植物中距离叶尖端大约8英寸(20cm)剪下(切下)叶。将叶样品(包括中脉)切成1英寸(2.5cm)片段并且置于50ml的样品小瓶中。样品随后置于干冰上直至转移到冰箱(≤-10℃)。将样品在≤-10℃冷冻运输和贮存直至抵达。将全部组织样品在真空下冻干直至干燥。制备时,将冻干的叶样品精细均化用于分析。样品在处理步骤之间冷冻贮存。
蛋白质浓度测定
使用特异性定量ELISA方法测定Cry1F、Cry34Ab1、Cry35Ab1和PAT蛋白的浓度。
蛋白质提取
处理的叶组织样品的等分试样以目标重量10mg称量至1.2mL管中。将用于Cry1F、Cry34Ab1、Cry35Ab1和PAT蛋白浓度分析的每个样品在0.6mL冰冷的PBST(磷酸盐缓冲盐水外加Tween-20)中萃取。离心后,将上清液取出、稀释并分析。
Cry1F、Cry34Ab1和PAT蛋白浓度的测定
所用的Cry1F、Cry34Ab1和PAT ELISA试剂盒从EnviroLogix,Inc.(Portland,ME)获得,并且所用的Cry35Ab1 ELISA试剂盒从Acadia BioScience,LLC(Portland,ME)获得。用于这四种蛋白质中每一种的ELISA方法利用顺序“夹心”模式以确定样品提取物中蛋白质的浓度。标准物(以三重孔分析)和稀释的样品提取物(以双孔分析)在用抗体预包被的平板上孵育,所述抗体是对选自Cry1F、Cry34Ab1、Cry35Ab1或PAT的单一蛋白质特异。孵育后,将未结合的物质从平板上洗去。将针对所选择的相应蛋白质的不同的特异性抗体(其与辣根过氧化物酶(HRP)缀合)添加至平板并且孵育。随后,将未结合的物质从平板洗去,从而留下“夹”在平板上包被的抗体与抗体-HRP缀合物之间的结合着的的蛋白质。通过添加在HRP存在下产生有色产物的底物完成对结合的抗体-蛋白质复合物的检测。该反应用酸溶液终止并且使用平板读数仪测定每个孔的光密度(OD)。用测自双孔的结果的平均值来确定以ng/mg样品干重计的Cry1F、Cry34Ab1、Cry35Ab1或PAT蛋白的浓度。
确定蛋白质浓度的计算
1.标准曲线
在每块ELISA平板上包括一标准曲线。用于该标准曲线的方程由该软件产生,所述软件使用二次拟合以使针对标准物所获得的平均OD值与相应的标准物浓度(ng/mL)相关。如下应用二次回归方程:
y=Cx2+Bx+A
其中x=已知的标准物浓度,而y=相应的平均吸光度值(OD)。
2.样品浓度
通过使用由标准曲线所确定的A、B和C的值,解算上述方程中的x,完成样品浓度(ng/ml)的内推。
例如,曲线参数:A=0.0476,B=0.4556,C=-0.01910并且样品OD=1.438
样品浓度值针对表述为1∶N的稀释系数作调整。
调整的浓度=样品浓度×稀释系数
例如,样品浓度=3.6ng/mL并且稀释系数=1∶10
调整的浓度=3.6ng/mL×10=36ng/mL
将调整的样品浓度值从ng/mL如下换算成ng/mg样品重量:
ng/mg样品重量=ng/mL×提取体积(mL)/样品重量(mg)
例如,浓度=36ng/mL,提取体积=0.60ml,并且
样品重量=10.0mg
ng/mg样品重量=36ng/mg×0.60mL/10.0mg=2.2ng/mg
3.定量下限(LLOQ)
以ng/mg样品重量计的LLOQ计算如下:
例如,对于叶中的PAT:可报告的分析LLOQ=2.3ng/mL,提取体积=0.6mL,并且样品靶重量=10mg
结果
在4114玉米的V9叶组织中检测到处于以下表14中所列浓度的蛋白质Cry1F、Cry34Ab1、Cry35Ab1和PAT。
表14:4114玉米中的蛋白质浓度
*Cry1F和PAT的LLOQ是0.14ng/mg干重;Cry34Ab1和Cry35Ab1的LLOQ是0.16ng/mg干重。
在解释并描述了本发明的原理之后,本领域技术人员应当清楚,本发明可以在安排和细节上进行修改而不脱离这些原理。我们要求保护处于所附权利要求书的精神和范围内的全部修改。
本说明书中引用的全部出版物及公开的专利文件均以引用方式以相同程度并入本文,一如专门且单独地说明通过引用方式并入每份单独的出版物或专利申请那样。
序列表
<110> 先锋国际良种公司
纳幕尔杜邦公司
<120> 玉米事件DP-004114-3及其检测方法
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Thr Tyr Ser Ser Thr Asn Ser Ile Gln Lys Trp Gln Ile Lys Ala Asn
85 90 95
Gly Ser Ser Tyr Val Ile Gln Ser Asp Asn Gly Lys Val Leu Thr Ala
100 105 110
Gly Thr Gly Gln Ala Leu Gly Leu Ile Arg Leu Thr Asp Glu Ser Ser
115 120 125
Asn Asn Pro Asn Gln Gln Trp Asn Leu Thr Ser Val Gln Thr Ile Gln
130 135 140
Leu Pro Gln Lys Pro Ile Ile Asp Thr Lys Leu Lys Asp Tyr Pro Lys
145 150 155 160
Tyr Ser Pro Thr Gly Asn Ile Asp Asn Gly Thr Ser Pro Gln Leu Met
165 170 175
Gly Trp Thr Leu Val Pro Cys Ile Met Val Asn Asp Pro Asn Ile Asp
180 185 190
Lys Asn Thr Gln Ile Lys Thr Thr Pro Tyr Tyr Ile Leu Lys Lys Tyr
195 200 205
Gln Tyr Trp Gln Arg Ala Val Gly Ser Asn Val Ala Leu Arg Pro His
210 215 220
Glu Lys Lys Ser Tyr Thr Tyr Glu Trp Gly Thr Glu Ile Asp Gln Lys
225 230 235 240
Thr Thr Ile Ile Asn Thr Leu Gly Phe Gln Ile Asn Ile Asp Ser Gly
245 250 255
Met Lys Phe Asp Ile Pro Glu Val Gly Gly Gly Thr Asp Glu Ile Lys
260 265 270
Thr Gln Leu Asn Glu Glu Leu Lys Ile Glu Tyr Ser His Glu Thr Lys
275 280 285
Ile Met Glu Lys Tyr Gln Glu Gln Ser Glu Ile Asp Asn Pro Thr Asp
290 295 300
Gln Ser Met Asn Ser Ile Gly Phe Leu Thr Ile Thr Ser Leu Glu Leu
305 310 315 320
Tyr Arg Tyr Asn Gly Ser Glu Ile Arg Ile Met Gln Ile Gln Thr Ser
325 330 335
Asp Asn Asp Thr Tyr Asn Val Thr Ser Tyr Pro Asn His Gln Gln Ala
340 345 350
Leu Leu Leu Leu Thr Asn His Ser Tyr Glu Glu Val Glu Glu Ile Thr
355 360 365
Asn Ile Pro Lys Ser Thr Leu Lys Lys Leu Lys Lys Tyr Tyr Phe
370 375 380
<210> 4
<211> 183
<212> PRT
<213> 绿色产色链霉菌
<220>
<221> 肽
<222> (0)...(0)
<223> 膦丝菌素乙酰基转移酶蛋白
<400> 4
Met Ser Pro Glu Arg Arg Pro Val Glu Ile Arg Pro Ala Thr Ala Ala
1 5 10 15
Asp Met Ala Ala Val Cys Asp Ile Val Asn His Tyr Ile Glu Thr Ser
20 25 30
Thr Val Asn Phe Arg Thr Glu Pro Gln Thr Pro Gln Glu Trp Ile Asp
35 40 45
Asp Leu Glu Arg Leu Gln Asp Arg Tyr Pro Trp Leu Val Ala Glu Val
50 55 60
Glu Gly Val Val Ala Gly Ile Ala Tyr Ala Gly Pro Trp Lys Ala Arg
65 70 75 80
Asn Ala Tyr Asp Trp Thr Val Glu Ser Thr Val Tyr Val Ser His Arg
85 90 95
His Gln Arg Leu Gly Leu Gly Ser Thr Leu Tyr Thr His Leu Leu Lys
100 105 110
Ser Met Glu Ala Gln Gly Phe Lys Ser Val Val Ala Val Ile Gly Leu
115 120 125
Pro Asn Asp Pro Ser Val Arg Leu His Glu Ala Leu Gly Tyr Thr Ala
130 135 140
Arg Gly Thr Leu Arg Ala Ala Gly Tyr Lys His Gly Gly Trp His Asp
145 150 155 160
Val Gly Phe Trp Gln Arg Asp Phe Glu Leu Pro Ala Pro Pro Arg Pro
165 170 175
Val Arg Pro Val Thr Gln Ile
180
<210> 5
<211> 11978
<212> DNA
<213> 人工序列
<220>
<223> 质粒PHP27118的T-DNA区的完整序列
<400> 5
gtttacccgc caatatatcc tgtcaaacac tgatagttta aacgctcttc aactggaaga 60
gcggttaccc ggaccgaagc ttcggccggg gcccatcgat atccgcgggc atgcctgcag 120
tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat 180
aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta 240
tacatatatt taaactttac tctacgaata atataatcta tagtactaca ataatatcag 300
tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt 360
tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc 420
aaatagcttc acctatataa tacttcatcc attttattag tacatccatt tagggtttag 480
ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc 540
taaattaaga aaactaaaac tctattttag tttttttatt taataattta gatataaaat 600
agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa 660
ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc 720
taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac 780
ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc 840
tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg 900
cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc caccgctcct 960
tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc 1020
aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc 1080
ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct 1140
agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt 1200
tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac 1260
gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc 1320
tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt 1380
tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt 1440
tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc 1500
ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg 1560
tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata 1620
ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg 1680
gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac 1740
tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct 1800
tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat 1860
gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac 1920
cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat 1980
acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg 2040
ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact 2100
tctgcaggtc gactctagag gatccaacaa tggagaacaa catacagaat cagtgcgtcc 2160
cctacaactg cctcaacaat cctgaagtag agattctcaa cgaagagagg tcgactggca 2220
gattgccgtt agacatctcc ctgtccctta cacgtttcct gttgtctgag tttgttccag 2280
gtgtgggagt tgcgtttggc ctcttcgacc tcatctgggg cttcatcact ccatctgatt 2340
ggagcctctt tcttctccag attgaacagt tgattgaaca aaggattgag accttggaaa 2400
ggaatcgggc catcactacc cttcgtggct tagcagacag ctatgagatc tacattgaag 2460
cactaagaga gtgggaagcc aatcctaaca atgcccaact gagagaagat gtgcgtatac 2520
gctttgctaa cacagatgat gctttgatca cagccatcaa caacttcacc cttaccagct 2580
tcgagatccc tcttctctcg gtctatgttc aagctgctaa cctgcacttg tcactactgc 2640
gcgacgctgt gtcgtttggg caaggttggg gactggacat agctactgtc aacaatcact 2700
acaacagact catcaatctg attcatcgat acacgaaaca ttgtttggat acctacaatc 2760
agggattgga gaacctgaga ggtactaaca ctcgccaatg ggccaggttc aatcagttca 2820
ggagagacct tacacttact gtgttagaca tagttgctct ctttccgaac tacgatgttc 2880
gtacctatcc gattcaaacg tcatcccaac ttacaaggga gatctacacc agttcagtca 2940
ttgaagactc tccagtttct gcgaacatac ccaatggttt caacagggct gagtttggag 3000
tcagaccacc ccatctcatg gacttcatga actctttgtt tgtgactgca gagactgtta 3060
gatcccaaac tgtgtgggga ggacacttag ttagctcacg caacacggct ggcaatcgta 3120
tcaactttcc tagttacggg gtcttcaatc ccgggggcgc catctggatt gcagatgaag 3180
atccacgtcc tttctatcgg accttgtcag atcctgtctt cgtccgagga ggctttggca 3240
atcctcacta tgtactcggt cttaggggag tggcctttca acaaactggt acgaatcaca 3300
cccgcacatt caggaactcc gggaccattg actctctaga tgagatacca cctcaagaca 3360
acagcggcgc accttggaat gactactccc atgtgctgaa tcatgttacc tttgtgcgct 3420
ggccaggtga gatctcaggt tccgactcat ggagagcacc aatgttctct tggacgcatc 3480
gtagcgctac ccccacaaac accattgatc cagagagaat cactcagatt cccttggtga 3540
aggcacacac acttcagtca ggaactacag ttgtaagagg gccggggttc acgggaggag 3600
acattcttcg acgcactagt ggaggaccat tcgcgtacac cattgtcaac atcaatgggc 3660
aacttcccca aaggtatcgt gccaggatac gctatgcctc tactaccaat ctaagaatct 3720
acgttacggt tgcaggtgaa cggatctttg ctggtcagtt caacaagaca atggataccg 3780
gtgatccact tacattccaa tctttctcct acgccactat caacaccgcg ttcacctttc 3840
caatgagcca gagcagtttc acagtaggtg ctgatacctt cagttcaggc aacgaagtgt 3900
acattgacag gtttgagttg attccagtta ctgccacact cgagtaagga tccgtcgacc 3960
tgcagccaag ctttcgcgag ctcgagatcc ccgacatatg ccccggtttc gttgcgacta 4020
acatgagttc ttggacaaat ttgattggac ctgatgagat gatccaaccc gaggatatag 4080
caaagctcgt tcgtgcagca atggaacggc caaaccgtgc ttttgtcccc aagaatgagg 4140
tgctatgcat gaaggaatct acccgttgat gtccaacagt ctcagggtta atgtctatgt 4200
atcttaaata atgttgtcgg tattttgtaa tctcatatag attttcactg tgcgacgcaa 4260
aaatattaaa taaatattat tattatctac gttttgattg agatatcatc aatattataa 4320
taaaaatatc cattaaacac gatttgatac aaatgacagt caataatctg atttgaatat 4380
ttattaattg taacgaatta cataaagatc gaatagaaaa tactgcactg caaatgaaaa 4440
ttaacacata ctaataaatg cgtcaaatat ctttgccaag atcaagcgga gtgagggcct 4500
catatccggt ctcagttaca agcacggtat ccccgaagcg cgctccacca atgccctcga 4560
catagatgcc gggctcgacg ctgaggacat tgcctacctt gagcatggtc tcagcgccgg 4620
ctttaagctc aatcccatcc caatctgaat atcctatccc gcgcccagtc cggtgtaaga 4680
acgggtctgt ccatccacct ctgttgggaa ttccggtccg ggtcaccttt gtccaccaag 4740
atggaactgc ggccagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 4800
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 4860
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 4920
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 4980
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5040
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5100
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5160
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5220
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5280
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 5340
atgccagcct gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc 5400
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 5460
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 5520
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 5580
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 5640
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 5700
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 5760
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tggttagggc 5820
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 5880
gctagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 5940
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6000
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6060
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6120
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6180
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6240
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6300
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 6360
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 6420
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 6480
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 6540
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 6600
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 6660
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 6720
gtcgatgctc accctgttgt ttggtgttac ttctgcaggt cgactctaga ggatccacac 6780
gacaccatgt ccgcccgcga ggtgcacatc gacgtgaaca acaagaccgg ccacaccctc 6840
cagctggagg acaagaccaa gctcgacggc ggcaggtggc gcacctcccc gaccaacgtg 6900
gccaacgacc agatcaagac cttcgtggcc gaatccaacg gcttcatgac cggcaccgag 6960
ggcaccatct actactcaat taatggcgag gccgagatca gcctctactt cgacaacccg 7020
ttcgccggct ccaacaaata cgacggccac tccaacaagt cccagtacga gatcatcacc 7080
cagggcggct ccggcaacca gtcccacgtg acctacacca tccagaccac ctcctcccgc 7140
tacggccaca agtcctgagt catgagtcat gagtcagtta acctagactt gtccatcttc 7200
tggattggcc aacttaatta atgtatgaaa taaaaggatg cacacatagt gacatgctaa 7260
tcactataat gtgggcatca aagttgtgtg ttatgtgtaa ttactagtta tctgaataaa 7320
agagaaagag atcatccata tttcttatcc taaatgaatg tcacgtgtct ttataattct 7380
ttgatgaacc agatgcattt cattaaccaa atccatatac atataaatat taatcatata 7440
taattaatat caattgggtt agcaaaacaa atctagtcta ggtgtgtttt gcgaatgcgg 7500
ccgcggaccg aattggggat ctgcatgaaa gaaactgtcg cactgctgaa ccgcaccttg 7560
tcactttcat cgaacacgac ctgtgcccaa gatgacggtg ctgcggtcta agtgaggctg 7620
aattgccttg gacagaagcg gactccctac aattagttag gccaaacggt gcatccatgt 7680
gtagctccgg gctcgggctg tatcgccatc tgcaatagca tccatggagc tcgttccatg 7740
tagttggaga tgaaccaatg atcgggcgtg tggacgtatg ttcctgtgta ctccgatagt 7800
agagtacgtg ttagctcttt catggtgcaa gtgaaatttg tgttggttta attaccccta 7860
cgttagttgc gggacaggag acacatcatg aatttaaagg cgatgatgtc ctctcctgta 7920
atgttattct tttgatgtga tgaatcaaaa tgtcatataa aacatttgtt gctctttagt 7980
taggcctgat cgtagaacga aatgctcgtg tagcggggct acgagcctat gacgcaataa 8040
cactggtttg ccggcccgga gtcgcttgac aaaaaaaagc atgttaagtt tatttacaat 8100
tcaaaaccta acatattata ttccctcaaa gcaggttcac gatcacacct gtacctaaaa 8160
aaaacatgaa gaatatatta ctccattatt atgagatgaa ccacttggca agagtggtaa 8220
gctatataaa aaaatgaaca ttattacgag atgttatatg ccattatatt gattcgaaga 8280
tatatgtttc tttctcccac gggcacctaa cggatacatg ataaggccaa ggcagatcac 8340
gggaaattat tcgaatacat gttacgccct attgccggaa aaaaaatgca gggcaggtgt 8400
tggccgtagc gatttaagca cttaagctgg aggttgccac acttggatgc aagcgtctga 8460
cccttctaaa aaatcggcgg ctttgtccgt atccgtatcc cctatccaac atctagctgg 8520
ccacacgacg gggctgggca gatcgtggat gccgggtcga cgtcgatcgt cagccatcat 8580
agaccaatcg accatctgtt atggatgctt gctagctaga ctagtcagac ataaaatttg 8640
gatactttct cccaactggg agacggggac tgatgtgcag ctgcacgtga gctaaatttt 8700
tccctataaa tatgcatgaa atactgcatt atcttgccac agccactgcc acagccagat 8760
aacaagtgca gctggtagca cgcaacgcat agctctggac ttgtagctag gtagccaacc 8820
ggatccacac gacaccatgc tcgacaccaa caaggtgtac gagatcagca accacgccaa 8880
cggcctctac gccgccacct acctctccct cgacgactcc ggcgtgtccc tcatgaacaa 8940
gaacgacgac gacatcgacg actacaacct caagtggttc ctcttcccga tcgacgacga 9000
ccagtacatc atcacctcct acgccgccaa caactgcaag gtgtggaacg tgaacaacga 9060
caagattaat gtgtcaacct actcctccac caactccatc cagaagtggc agatcaaggc 9120
caacggctcc tcctacgtga tccagtccga caacggcaag gtgctcaccg ccggcaccgg 9180
ccaggccctc ggcctcatcc gcctcaccga cgagtcctcc aacaacccga accagcaatg 9240
gaacctgacg tccgtgcaga ccatccagct cccgcagaag ccgatcatcg acaccaagct 9300
caaggactac ccgaagtact ccccgaccgg caacatcgac aacggcacct ccccgcagct 9360
catgggctgg accctcgtgc cgtgcatcat ggtgaacgac ccgaacatcg acaagaacac 9420
ccagatcaag accaccccgt actacatcct caagaagtac cagtactggc agagggccgt 9480
gggctccaac gtcgcgctcc gcccgcacga gaagaagtcc tacacctacg agtggggcac 9540
cgagatcgac cagaagacca ccatcatcaa caccctcggc ttccagatca acatcgacag 9600
cggcatgaag ttcgacatcc cggaggtggg cggcggtacc gacgagatca agacccagct 9660
caacgaggag ctcaagatcg agtattcaca tgagacgaag atcatggaga agtaccagga 9720
gcagtccgag atcgacaacc cgaccgacca gtccatgaac tccatcggct tcctcaccat 9780
cacctccctg gagctctacc gctacaacgg ctccgagatc cgcatcatgc agatccagac 9840
ctccgacaac gacacctaca acgtgacctc ctacccgaac caccagcagg ccctgctgct 9900
gctgaccaac cactcctacg aggaggtgga ggagatcacc aacatcccga agtccaccct 9960
caagaagctc aagaagtact acttctgagt catgagtcat gagtcagtta acctagactt 10020
gtccatcttc tggattggcc aacttaatta atgtatgaaa taaaaggatg cacacatagt 10080
gacatgctaa tcactataat gtgggcatca aagttgtgtg ttatgtgtaa ttactagtta 10140
tctgaataaa agagaaagag atcatccata tttcttatcc taaatgaatg tcacgtgtct 10200
ttataattct ttgatgaacc agatgcattt cattaaccaa atccatatac atataaatat 10260
taatcatata taattaatat caattgggtt agcaaaacaa atctagtcta ggtgtgtttt 10320
gcgaattatc gatgggcccc ggccgaagct ggccgcggac cgaattccca tggagtcaaa 10380
gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag 10440
tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacgct 10500
tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt 10560
tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt 10620
tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg 10680
aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac 10740
gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 10800
tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc 10860
ctctatataa ggaagttcat ttcatttgga gaggacaggg tacccgggga tccaccatgt 10920
ctccggagag gagaccagtt gagattaggc cagctacagc agctgatatg gccgcggttt 10980
gtgatatcgt taaccattac attgagacgt ctacagtgaa ctttaggaca gagccacaaa 11040
caccacaaga gtggattgat gatctagaga ggttgcaaga tagataccct tggttggttg 11100
ctgaggttga gggtgttgtg gctggtattg cttacgctgg gccctggaag gctaggaacg 11160
cttacgattg gacagttgag agtactgttt acgtgtcaca taggcatcaa aggttgggcc 11220
taggatccac attgtacaca catttgctta agtctatgga ggcgcaaggt tttaagtctg 11280
tggttgctgt tataggcctt ccaaacgatc catctgttag gttgcatgag gctttgggat 11340
acacagcccg gggtacattg cgcgcagctg gatacaagca tggtggatgg catgatgttg 11400
gtttttggca aagggatttt gagttgccag ctcctccaag gccagttagg ccagttaccc 11460
agatctgagt cgacctgcag gcatgcccgc tgaaatcacc agtctctctc tacaaatcta 11520
tctctctcta taataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg 11580
tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac 11640
ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gggcgagctc gaattcgagc 11700
tcgagcccgg gtggatcctc tagagtcgac ctgcagaagc ttcggtccgg cgcgcctcta 11760
gttgaagaca cgttcatgtc ttcatcgtaa gaagacactc agtagtcttc ggccagaatg 11820
gcctaactca aggccatcgt ggcctcttgc tcttcaggat gaagagctat gtttaaacgt 11880
gcaagcgcta ctagacaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc 11940
taagcgtcaa tttgtttaca ccacaatata tcctgcca 11978
<210> 6
<211> 16752
<212> DNA
<213> 人工序列
<220>
<223> 事件DP-004114-3的插入物和侧翼区的完整序列
<400> 6
gagcatatcc agcaccagct ggtaccaagg tcgggtctct gtgctagtgc tattagctag 60
tgtaaggagc gagtaggtca gttaaggctg gtgcgtcgtg agggctgtct tgtgtgtagc 120
tacagcagac ggttcatcag aaggattatt cgtgcagtat atacagtaca actagacaat 180
gatgttgatg attggtctag agctagaggc ctatagccct atactactgt gtattgtccg 240
ccgttttagt tttttggtcc catcccatca atgcaaccgc cttgttttgc tccaattgtc 300
ccgttcctgc gcctcgcttt tgctctgtcg catcgcatac aaaaaaaaaa acgccgcgcc 360
ggctttgaat cgcgcccccc aactgctcca accaggcaac ggacacggcc accgtccgtg 420
tcgcgagcaa aaaaacaaaa agaggaacgc gtccaggacg aagcagtcca ctgccgctgt 480
ggccggcaaa agatctggtt gagcacatgg agattggaga aggttggttg gttcttctgg 540
aaacgccaat gaatgggggc actgacatgt actcttaaca tgtagtgcaa tccagagatc 600
ggatatccag acactggcag cacgatcgcc tcgcgccgta gatcacgcac gcaaattact 660
gaagaccatt cacaaaaaaa aaaaaacaca caggggctag cgtgccccac accaaaccca 720
agtgctgcgt tgcacgcagg ggagcgaaaa aaaacaataa tgctcactgt cacgtcgcgt 780
atccaacccc gcggacgtct cggctctcag cagcagcaca cggggcacct cacgatgccg 840
ttctcgttgc actccgtgca ccgccggaac ccgccgccgc attcgtcgcc ctcctcctcc 900
tcctccgcct cgtcttcgtc acccacgtac accttgcagc tgcccgagca gacatcgcag 960
agcacgaacc gcatgtcccc gcaggcctcg cacgcgccgg cgtcgccgcc gtgtgggccg 1020
gccgtcgacg cagcgctctc gcacccggcc agcctcggcg cgagctcccc ggcctcgtgc 1080
agccgcttca gctcctcggc gttgcccacg agctccccgt ccacgaagag gctggggagg 1140
gcggcgggcg tgccgccggc ttggccgagc ccgaggccga gaaggccgcg gagctcgtcc 1200
cggaacccgc ggtgcatgga cacgtcgcgc tcgtcgaggc gcacgccgta gcccttgagg 1260
atggcgcgcg ccaggcagca gtcctcgtgc gtggcgcgca cgccgcgcag cgacgtgaag 1320
tagagcaccg ccctccgcgg cggcagcgcc ttcccctccc cgccgctcgt cggggcggcg 1380
tcgggccgag gcatcggcat cggcagcggc gtcaccttgg cggacgccgc gaggtcctgc 1440
gcaggcgccg tggcgaccgg gaacgagaag gagtggcgcc cgaacggcgc gcccagcagc 1500
ggggagcggt cctcgaggcc ggccatgagc gcccacgcgt cgatgtcctc gggctcgttg 1560
ggcggcgtca tggtgggcgt gcgcggcgcc agcctcgtgg gcgcgggctc cggcgcccgc 1620
ggcagggcct tgtccagctc cagggacccg agcgtggacg acgtgagccg caccacgtgg 1680
acgccgacgt cgctggggca ccgagccggg aacgactggc tgcgcggcag cggtgacggg 1740
cagtaccgga ggtcgtgacg ggcctgcctt gaggtggtgc accccatggc accaatgtac 1800
acacacggcc aaagcgccaa gtgggctgca gactgcctgc caatgtgatc aagcagccag 1860
gagcagagac ggatctctgg ggatcggggt ttctggggtt taggatcttt atactactct 1920
gtcattgggg atataaaact aggagtgtgg ttaattagga ctcgatagat aagtttacca 1980
caagcgcgtg aaatggtcta cccgatgatg tgattggcct aaaaagaaca agaagagtat 2040
ttggagctac tgaacattct cttttcctga agataactaa tttttggaac attcagactt 2100
gggagtctgg acttttggag ggaagttcaa attgtggtct gcctctgcca tgtgttgttt 2160
tttagtcgga gagtggccct catttttttt gtcctgttta gctttatagt cgtagcagct 2220
agtagcgaaa tttaaccttg gattatggcc gtgttagtca aacaatcatt gatttatttc 2280
ctccctttcg cgctgctttt cctgtacgca tctccgctgc ccttgattcg aggaccctgt 2340
tcacaacaca gggctctggc tttggagcct ctcgtttgta gcacttgcac gtagttaccc 2400
ggaccgaagc ttcaacacag atctgatagt ttaaacgctc ttcaactgga agagcggtta 2460
cccggaccga agcttcggcc ggggcccatc gatatccgcg ggcatgcctg cagtgcagcg 2520
tgacccggtc gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat 2580
taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat 2640
atttaaactt tactctacga ataatataat ctatagtact acaataatat cagtgtttta 2700
gagaatcata taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac 2760
aggactctac agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc 2820
ttcacctata taatacttca tccattttat tagtacatcc atttagggtt tagggttaat 2880
ggtttttata gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta 2940
agaaaactaa aactctattt tagttttttt atttaataat ttagatataa aatagaataa 3000
aataaagtga ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca 3060
tttttcttgt ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga 3120
caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct 3180
ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg 3240
tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc 3300
ctcctcctct cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt 3360
cccttcctcg cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg 3420
tgttgttcgg agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct 3480
ccgcttcaag gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg 3540
cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc 3600
gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac 3660
acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc 3720
gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg 3780
cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct 3840
tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag 3900
aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata 3960
catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac 4020
atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga 4080
tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca 4140
aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt 4200
tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt 4260
ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt 4320
acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga 4380
tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta 4440
tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag 4500
gtcgactcta gaggatccaa caatggagaa caacatacag aatcagtgcg tcccctacaa 4560
ctgcctcaac aatcctgaag tagagattct caacgaagag aggtcgactg gcagattgcc 4620
gttagacatc tccctgtccc ttacacgttt cctgttgtct gagtttgttc caggtgtggg 4680
agttgcgttt ggcctcttcg acctcatctg gggcttcatc actccatctg attggagcct 4740
ctttcttctc cagattgaac agttgattga acaaaggatt gagaccttgg aaaggaatcg 4800
ggccatcact acccttcgtg gcttagcaga cagctatgag atctacattg aagcactaag 4860
agagtgggaa gccaatccta acaatgccca actgagagaa gatgtgcgta tacgctttgc 4920
taacacagat gatgctttga tcacagccat caacaacttc acccttacca gcttcgagat 4980
ccctcttctc tcggtctatg ttcaagctgc taacctgcac ttgtcactac tgcgcgacgc 5040
tgtgtcgttt gggcaaggtt ggggactgga catagctact gtcaacaatc actacaacag 5100
actcatcaat ctgattcatc gatacacgaa acattgtttg gatacctaca atcagggatt 5160
ggagaacctg agaggtacta acactcgcca atgggccagg ttcaatcagt tcaggagaga 5220
ccttacactt actgtgttag acatagttgc tctctttccg aactacgatg ttcgtaccta 5280
tccgattcaa acgtcatccc aacttacaag ggagatctac accagttcag tcattgaaga 5340
ctctccagtt tctgcgaaca tacccaatgg tttcaacagg gctgagtttg gagtcagacc 5400
accccatctc atggacttca tgaactcttt gtttgtgact gcagagactg ttagatccca 5460
aactgtgtgg ggaggacact tagttagctc acgcaacacg gctggcaatc gtatcaactt 5520
tcctagttac ggggtcttca atcccggggg cgccatctgg attgcagatg aagatccacg 5580
tcctttctat cggaccttgt cagatcctgt cttcgtccga ggaggctttg gcaatcctca 5640
ctatgtactc ggtcttaggg gagtggcctt tcaacaaact ggtacgaatc acacccgcac 5700
attcaggaac tccgggacca ttgactctct agatgagata ccacctcaag acaacagcgg 5760
cgcaccttgg aatgactact cccatgtgct gaatcatgtt acctttgtgc gctggccagg 5820
tgagatctca ggttccgact catggagagc accaatgttc tcttggacgc atcgtagcgc 5880
tacccccaca aacaccattg atccagagag aatcactcag attcccttgg tgaaggcaca 5940
cacacttcag tcaggaacta cagttgtaag agggccgggg ttcacgggag gagacattct 6000
tcgacgcact agtggaggac cattcgcgta caccattgtc aacatcaatg ggcaacttcc 6060
ccaaaggtat cgtgccagga tacgctatgc ctctactacc aatctaagaa tctacgttac 6120
ggttgcaggt gaacggatct ttgctggtca gttcaacaag acaatggata ccggtgatcc 6180
acttacattc caatctttct cctacgccac tatcaacacc gcgttcacct ttccaatgag 6240
ccagagcagt ttcacagtag gtgctgatac cttcagttca ggcaacgaag tgtacattga 6300
caggtttgag ttgattccag ttactgccac actcgagtaa ggatccgtcg acctgcagcc 6360
aagctttcgc gagctcgaga tccccgacat atgccccggt ttcgttgcga ctaacatgag 6420
ttcttggaca aatttgattg gacctgatga gatgatccaa cccgaggata tagcaaagct 6480
cgttcgtgca gcaatggaac ggccaaaccg tgcttttgtc cccaagaatg aggtgctatg 6540
catgaaggaa tctacccgtt gatgtccaac agtctcaggg ttaatgtcta tgtatcttaa 6600
ataatgttgt cggtattttg taatctcata tagattttca ctgtgcgacg caaaaatatt 6660
aaataaatat tattattatc tacgttttga ttgagatatc atcaatatta taataaaaat 6720
atccattaaa cacgatttga tacaaatgac agtcaataat ctgatttgaa tatttattaa 6780
ttgtaacgaa ttacataaag atcgaataga aaatactgca ctgcaaatga aaattaacac 6840
atactaataa atgcgtcaaa tatctttgcc aagatcaagc ggagtgaggg cctcatatcc 6900
ggtctcagtt acaagcacgg tatccccgaa gcgcgctcca ccaatgccct cgacatagat 6960
gccgggctcg acgctgagga cattgcctac cttgagcatg gtctcagcgc cggctttaag 7020
ctcaatccca tcccaatctg aatatcctat cccgcgccca gtccggtgta agaacgggtc 7080
tgtccatcca cctctgttgg gaattccggt ccgggtcacc tttgtccacc aagatggaac 7140
tgcggccagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc tctagagata 7200
atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt 7260
tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg aataatataa 7320
tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat 7380
ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt 7440
gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta 7500
ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca 7560
tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt 7620
tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac 7680
cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag 7740
cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt 7800
cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga 7860
gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg 7920
gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg 7980
ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac 8040
accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc 8100
agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc 8160
cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag ggcccggtag 8220
ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg 8280
ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt gccagtgttt 8340
ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt 8400
ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt 8460
gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt 8520
ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc tggtggattt 8580
attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat 8640
ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat 8700
acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat 8760
tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga 8820
actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga 8880
tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg 8940
cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt 9000
ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga 9060
tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg 9120
ctcaccctgt tgtttggtgt tacttctgca ggtcgactct agaggatcca cacgacacca 9180
tgtccgcccg cgaggtgcac atcgacgtga acaacaagac cggccacacc ctccagctgg 9240
aggacaagac caagctcgac ggcggcaggt ggcgcacctc cccgaccaac gtggccaacg 9300
accagatcaa gaccttcgtg gccgaatcca acggcttcat gaccggcacc gagggcacca 9360
tctactactc aattaatggc gaggccgaga tcagcctcta cttcgacaac ccgttcgccg 9420
gctccaacaa atacgacggc cactccaaca agtcccagta cgagatcatc acccagggcg 9480
gctccggcaa ccagtcccac gtgacctaca ccatccagac cacctcctcc cgctacggcc 9540
acaagtcctg agtcatgagt catgagtcag ttaacctaga cttgtccatc ttctggattg 9600
gccaacttaa ttaatgtatg aaataaaagg atgcacacat agtgacatgc taatcactat 9660
aatgtgggca tcaaagttgt gtgttatgtg taattactag ttatctgaat aaaagagaaa 9720
gagatcatcc atatttctta tcctaaatga atgtcacgtg tctttataat tctttgatga 9780
accagatgca tttcattaac caaatccata tacatataaa tattaatcat atataattaa 9840
tatcaattgg gttagcaaaa caaatctagt ctaggtgtgt tttgcgaatg cggccgcgga 9900
ccgaattggg gatctgcatg aaagaaactg tcgcactgct gaaccgcacc ttgtcacttt 9960
catcgaacac gacctgtgcc caagatgacg gtgctgcggt ctaagtgagg ctgaattgcc 10020
ttggacagaa gcggactccc tacaattagt taggccaaac ggtgcatcca tgtgtagctc 10080
cgggctcggg ctgtatcgcc atctgcaata gcatccatgg agctcgttcc atgtagttgg 10140
agatgaacca atgatcgggc gtgtggacgt atgttcctgt gtactccgat agtagagtac 10200
gtgttagctc tttcatggtg caagtgaaat ttgtgttggt ttaattaccc ctacgttagt 10260
tgcgggacag gagacacatc atgaatttaa aggcgatgat gtcctctcct gtaatgttat 10320
tcttttgatg tgatgaatca aaatgtcata taaaacattt gttgctcttt agttaggcct 10380
gatcgtagaa cgaaatgctc gtgtagcggg gctacgagcc tatgacgcaa taacactggt 10440
ttgccggccc ggagtcgctt gacaaaaaaa agcatgttaa gtttatttac aattcaaaac 10500
ctaacatatt atattccctc aaagcaggtt cacgatcaca cctgtaccta aaaaaaacat 10560
gaagaatata ttactccatt attatgagat gaaccacttg gcaagagtgg taagctatat 10620
aaaaaaatga acattattac gagatgttat atgccattat attgattcga agatatatgt 10680
ttctttctcc cacgggcacc taacggatac atgataaggc caaggcagat cacgggaaat 10740
tattcgaata catgttacgc cctattgccg gaaaaaaaat gcagggcagg tgttggccgt 10800
agcgatttaa gcacttaagc tggaggttgc cacacttgga tgcaagcgtc tgacccttct 10860
aaaaaatcgg cggctttgtc cgtatccgta tcccctatcc aacatctagc tggccacacg 10920
acggggctgg gcagatcgtg gatgccgggt cgacgtcgat cgtcagccat catagaccaa 10980
tcgaccatct gttatggatg cttgctagct agactagtca gacataaaat ttggatactt 11040
tctcccaact gggagacggg gactgatgtg cagctgcacg tgagctaaat ttttccctat 11100
aaatatgcat gaaatactgc attatcttgc cacagccact gccacagcca gataacaagt 11160
gcagctggta gcacgcaacg catagctctg gacttgtagc taggtagcca accggatcca 11220
cacgacacca tgctcgacac caacaaggtg tacgagatca gcaaccacgc caacggcctc 11280
tacgccgcca cctacctctc cctcgacgac tccggcgtgt ccctcatgaa caagaacgac 11340
gacgacatcg acgactacaa cctcaagtgg ttcctcttcc cgatcgacga cgaccagtac 11400
atcatcacct cctacgccgc caacaactgc aaggtgtgga acgtgaacaa cgacaagatt 11460
aatgtgtcaa cctactcctc caccaactcc atccagaagt ggcagatcaa ggccaacggc 11520
tcctcctacg tgatccagtc cgacaacggc aaggtgctca ccgccggcac cggccaggcc 11580
ctcggcctca tccgcctcac cgacgagtcc tccaacaacc cgaaccagca atggaacctg 11640
acgtccgtgc agaccatcca gctcccgcag aagccgatca tcgacaccaa gctcaaggac 11700
tacccgaagt actccccgac cggcaacatc gacaacggca cctccccgca gctcatgggc 11760
tggaccctcg tgccgtgcat catggtgaac gacccgaaca tcgacaagaa cacccagatc 11820
aagaccaccc cgtactacat cctcaagaag taccagtact ggcagagggc cgtgggctcc 11880
aacgtcgcgc tccgcccgca cgagaagaag tcctacacct acgagtgggg caccgagatc 11940
gaccagaaga ccaccatcat caacaccctc ggcttccaga tcaacatcga cagcggcatg 12000
aagttcgaca tcccggaggt gggcggcggt accgacgaga tcaagaccca gctcaacgag 12060
gagctcaaga tcgagtattc acatgagacg aagatcatgg agaagtacca ggagcagtcc 12120
gagatcgaca acccgaccga ccagtccatg aactccatcg gcttcctcac catcacctcc 12180
ctggagctct accgctacaa cggctccgag atccgcatca tgcagatcca gacctccgac 12240
aacgacacct acaacgtgac ctcctacccg aaccaccagc aggccctgct gctgctgacc 12300
aaccactcct acgaggaggt ggaggagatc accaacatcc cgaagtccac cctcaagaag 12360
ctcaagaagt actacttctg agtcatgagt catgagtcag ttaacctaga cttgtccatc 12420
ttctggattg gccaacttaa ttaatgtatg aaataaaagg atgcacacat agtgacatgc 12480
taatcactat aatgtgggca tcaaagttgt gtgttatgtg taattactag ttatctgaat 12540
aaaagagaaa gagatcatcc atatttctta tcctaaatga atgtcacgtg tctttataat 12600
tctttgatga accagatgca tttcattaac caaatccata tacatataaa tattaatcat 12660
atataattaa tatcaattgg gttagcaaaa caaatctagt ctaggtgtgt tttgcgaatt 12720
atcgatgggc cccggccgaa gctggccgcg gaccgaattc ccatggagtc aaagattcaa 12780
atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta 12840
cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac gcttgtctac 12900
tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa 12960
agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg 13020
aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc 13080
atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 13140
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc 13200
tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata 13260
taaggaagtt catttcattt ggagaggaca gggtacccgg ggatccacca tgtctccgga 13320
gaggagacca gttgagatta ggccagctac agcagctgat atggccgcgg tttgtgatat 13380
cgttaaccat tacattgaga cgtctacagt gaactttagg acagagccac aaacaccaca 13440
agagtggatt gatgatctag agaggttgca agatagatac ccttggttgg ttgctgaggt 13500
tgagggtgtt gtggctggta ttgcttacgc tgggccctgg aaggctagga acgcttacga 13560
ttggacagtt gagagtactg tttacgtgtc acataggcat caaaggttgg gcctaggatc 13620
cacattgtac acacatttgc ttaagtctat ggaggcgcaa ggttttaagt ctgtggttgc 13680
tgttataggc cttccaaacg atccatctgt taggttgcat gaggctttgg gatacacagc 13740
ccggggtaca ttgcgcgcag ctggatacaa gcatggtgga tggcatgatg ttggtttttg 13800
gcaaagggat tttgagttgc cagctcctcc aaggccagtt aggccagtta cccagatctg 13860
agtcgacctg caggcatgcc cgctgaaatc accagtctct ctctacaaat ctatctctct 13920
ctataataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct 13980
catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc 14040
aataaaattt ctaattccta aaaccaaaat ccagggcgag ctcgaattcg agctcgagcc 14100
cgggtggatc ctctagagtc gacctgcaga agcttcggtc cggcgcgcct ctagttgaag 14160
acacgttcat gtcttcatcg taagaagaca ctcagtagtc ttcggccaga atggcctaac 14220
tcaaggccat cgtggcctct tgctcttcag gatgaagagc tatgtttaaa cgtgcaagcg 14280
ctactagaca attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 14340
caatttggaa caagtggcta tcgccagata taagaacttc gatccgaaat atcgtttcaa 14400
aactagaaaa cagcgcggct ttggctaagc cgcgcactat ataggatttt gggcaccttt 14460
tgatggaacg tgaaagcgta ctgcgcacta gttatttagg ttgaaccttg gatatacggt 14520
tctcactgcg ccaatgcaag gcttgaaact tggttagtaa tacgtactcc ctccgtttct 14580
ttttatttgt cgctggatag tgcaattttg cactatcgag cgacaaataa aaagaaacgg 14640
agggagtata tgattgtcag atgtagatat gtttatttat atatcacata cagatatata 14700
aaacagatca ctttttcaga tatacagttc caatgtcagc cctgatcacc ctgtcataaa 14760
ttgcacgttt ctaattgatg ttgcttcatg gtcgtcatga gaaccttctg aagaaatcga 14820
tgaaggttgc caacctttca aagtttcaga aaccactttg catgtacact aagggctggt 14880
ttggcagccc aaaaccagcc agcgttttcc tggtcttttc tcccgggaga aagcccatgc 14940
atagattgtc cctggattat ttatctgtgt cctttggcta aaaattcgtc ccaatttcct 15000
gtaggaaact acctcggcct tgggaggcca ggcgattctc caccgcctcg tctcgtccat 15060
ccttcgatgc tcacgcgtgc ctcctcggat gctatcctca ggcgattctc cgtcgtctcg 15120
tctcatccat cctcacgcgc gcctcctccg acgctatccc caggcgattc tccaccgtct 15180
cgtctcatcc atcctcatgt acgcctcgtc cgatgctatc cccagacgat tttccgtcgt 15240
ctcatctcct tcatgctcgc gcgcgcctcc tccgacgcta tccccaggcg atttttctgc 15300
cgtctcgtct ccttcatgcc cgcgcgcgcc tcctccgacg ctatccccag gcgattttcc 15360
gccgtctcgt ctccttcatg cccgcgcgtg cctcctccga cgctattccc acgagcgcct 15420
ccgccgccgc tatccccaga cgattttccg ctgtctcgtc tccttcatgc ccgcgcgccc 15480
ctcctccgac gctatcccca cgagcgcctc cgccgccgct ccaccgtctt ccccgccgcc 15540
atccccttaa ttcctataga tctggacccc gctctacttt cgttggcata cttttgcttg 15600
gtgtgcgcgg gctggagtgg aaggttgcgc attcgatcac gggggagaag tggatcttgg 15660
gtcttggcag gctagggcgg ttgccaggac gccgtggtgt gcattcatgg gtcctataaa 15720
tctttatcat taccgcctta ggagctagtt gtagttcaca catcatatcc ttttctgctc 15780
gacatcgtct ggggatgccc taggtgccct accgacccta cggcattgtc ttgacctcta 15840
ttagactcta tgtcatctag agccttcttg ggtggccttt tgaccccaaa gcgaccctat 15900
gatcttaccc taacgaggtc tcccttggtg gggcaagatc cactttgtcc acttaactga 15960
agatctgatc ctcatcttga aatctttaat cccaaggtga ctctacgtcg tatgtggatg 16020
ctccgggtaa cctgccaacc cggatcaccc taagatctct ttcctaaggg gcgagatcta 16080
ggttcctacg agaaagaaga cgaccctgca ccattgcggt ccgtccggtc cagagtgcga 16140
acgtccggat gcgacacagg gaaggagtcg ctcctgcagc gaggtcgcag actgtccaca 16200
cagcctcaga aggcaccgcc agacaataca tgtaataccc actctgtaag aaaaacctaa 16260
aaggagaaag tatattcctt tatctatatg tgtgttatat ttctactcac catcacatgt 16320
gaacatctca cttacacaaa taaataatta acaaaagaca ctcaaataaa ttatgcatca 16380
tgctcgacct tattttgtgt gcattctgtt acaatataaa aataatataa aaaacatata 16440
ttaatatcaa aatttggaga tttaacccta atatgcaaat cggagtttag aggaaagaaa 16500
gaaaaatgct atacaaaata aaggaataaa tatataaata aaggtaaaac tattaatact 16560
ggtatattaa tttgaacagt tgacctaatt atgaatatca caactggttt gaattcaaat 16620
atgaaatcca agaatttgga aataggaaaa atggagataa gaataaagga aaagaattct 16680
taactcggat gggcctggga aacgaatttc ggcccacttc ctgtgtcctt agctgtgcgg 16740
ctcagtccag tg 16752
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Claims (10)
1.一种DNA构建体,其包含:第一、第二、第三和第四表达盒,其中所述第一表达盒包括处于可操作地连接的
(a)玉米遍在蛋白启动子;
(b)玉米遍在蛋白基因的5'非翻译外显子;
(c)玉米遍在蛋白第一内含子;
(d)编码Cry1F的DNA分子;和
(e)来自ORF 25终止子的多聚(A)添加信号;
所述第二表达盒包括处于可操作地连接的
(1)玉米遍在蛋白启动子;
(2)玉米遍在蛋白基因的5'非翻译外显子;
(3)玉米遍在蛋白第一内含子;
(4)编码Cry34Ab1的DNA分子;和
(5)PinII转录终止子;
所述第三表达盒包括处于可操作地连接的
(i)小麦过氧化物酶启动子;
(ii)编码Cry35Ab1的DNA分子;和
(iii)PinII转录终止子;并且
所述第四表达盒包括处于可操作地连接的
(a)CaMV 35S启动子;
(b)编码pat的DNA分子;和
(c)来自CaMV 35S的3'转录终止子。
2.一种植物,其包含根据权利要求1所述的DNA构建体。
3.根据权利要求2所述的植物,其中所述植物是玉米植物。
4.一种植物,其包含SEQ ID NO:6中所述的序列。
5.一种玉米植物,其包含玉米事件DP-004114-3的基因型,其中所述基因型包含SEQ IDNO:27和SEQ ID NO:28中所述的核苷酸序列。
6.根据权利要求5所述的玉米植物,其中所述基因型包含SEQ ID NO:27中所述的核苷酸序列。
7.根据权利要求5所述的玉米植物,其中所述基因型包含SEQ ID NO:28中所述的核苷酸序列。
8.一种玉米事件DP-004114-3,其中所述玉米事件的种子的代表性样品已经以登录号PTA-11506保藏于美国典型培养物保藏中心(ATCC)。
9.根据权利要求8所述的玉米事件的植物部分。
10.包含玉米事件DP-004114-3的种子,其中所述种子包含选自SEQ ID NO:27和SEQ IDNO:28的DNA分子,其中玉米事件DP-004114-3种子的代表性样品已经以登录号PTA-11506保藏于美国典型培养物保藏中心(ATCC)。
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EP (1) | EP2512226B1 (zh) |
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CN116640761A (zh) * | 2023-07-20 | 2023-08-25 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp018-1及其检测方法 |
CN117144054A (zh) * | 2023-10-27 | 2023-12-01 | 莱肯生物科技(海南)有限公司 | 一种核酸检测方法及其应用 |
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AR090558A1 (es) * | 2012-04-24 | 2014-11-19 | Pioneer Hi Bred Int | Evento de maiz dp-004114-3 y metodos para su deteccion |
EP2662362A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
EP2662364A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole tetrahydronaphthyl carboxamides |
JP6262208B2 (ja) | 2012-05-09 | 2018-01-17 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | ピラゾールインダニルカルボキサミド類 |
EP2662363A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole biphenylcarboxamides |
EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
MX2014013489A (es) | 2012-05-09 | 2015-02-12 | Bayer Cropscience Ag | 5-halogenopirazolindanil carboxamidas. |
EP2662370A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole benzofuranyl carboxamides |
EP2662360A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole indanyl carboxamides |
AR091104A1 (es) | 2012-05-22 | 2015-01-14 | Bayer Cropscience Ag | Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida |
EP3409120A1 (en) | 2012-05-30 | 2018-12-05 | Bayer CropScience Aktiengesellschaft | Composition comprising a biological control agent and a fungicide |
MX362859B (es) | 2012-05-30 | 2019-02-20 | Bayer Cropscience Ag | Composiciones que comprenden un agente de control biologico y un insecticida. |
PL2854549T3 (pl) | 2012-05-30 | 2019-02-28 | Bayer Cropscience Ag | Kompozycja zawierająca środek kontroli biologicznej i fluopikolid |
PT2854547T (pt) | 2012-05-30 | 2018-11-16 | Bayer Cropscience Ag | Composição que compreende um agente de controlo biológico e trifloxistrobina |
EP3205210A1 (en) | 2012-05-30 | 2017-08-16 | Bayer CropScience Aktiengesellschaft | Composition comprising a biological control agent and a fungicide selected from inhibitors of the succinate dehydrogenase |
TR201816247T4 (tr) | 2012-05-30 | 2018-11-21 | Bayer Cropscience Ag | Metalaksil ve metalaksil-m'den seçilen bir fungisit ve bir biyolojik kontrol ajanı içeren bileşim. |
MX356530B (es) | 2012-05-30 | 2018-06-01 | Bayer Cropscience Ag | Composicion que comprende un agente de control biologico y un fungicida seleccionado de inhibidores de la biosintesis de aminoacidos o proteinas, inhibidores de la produccion de atp e inhibidores de la sintesis de la pared celular. |
ES2698951T3 (es) | 2012-05-30 | 2019-02-06 | Bayer Cropscience Ag | Composiciones que comprenden un agente de control biológico y un insecticida |
MX2015001456A (es) | 2012-07-31 | 2015-05-08 | Bayer Cropscience Ag | Composicion que comprende una mezcla de terpenos plaguicida y un insecticida. |
UY35035A (es) | 2012-09-14 | 2014-04-30 | Bayer Cropscience Ag | ?molécula de ácido nucleico recombinante que codifica una enzima hppd, vector, célula huésped, semilla, planta, polipéptido, producto primario, métodos y usos?. |
EP2719280A1 (en) | 2012-10-11 | 2014-04-16 | Bayer CropScience AG | Use of N-phenylethylpyrazole carboxamide derivatives or salts thereof for resistance management of phytopathogenic fungi |
JP6262747B2 (ja) | 2012-10-19 | 2018-01-17 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | カルボキサミド誘導体を用いる植物成長促進方法 |
US9801374B2 (en) | 2012-10-19 | 2017-10-31 | Bayer Cropscience Ag | Active compound combinations comprising carboxamide derivatives |
UA114647C2 (uk) | 2012-10-19 | 2017-07-10 | Байєр Кропсайнс Аг | Спосіб поліпшення стійкості рослин до абіотичного стресу з використанням карбоксамідних або тіокарбоксамідних похідних |
DK2908641T3 (da) | 2012-10-19 | 2018-04-23 | Bayer Cropscience Ag | Fremgangsmåde til behandling af planter mod svampe, der er resistente over for fungicider, ved anvendelse af carboxamid- eller thiocarboxamidderivater |
EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
MX2015006328A (es) | 2012-11-30 | 2015-09-07 | Bayer Cropscience Ag | Mezcla fungicida o pesticida binaria. |
UA116222C2 (uk) | 2012-11-30 | 2018-02-26 | Байєр Кропсайєнс Акцієнгезелльшафт | Потрійні фунгіцидні і пестицидні суміші |
EA030020B1 (ru) | 2012-11-30 | 2018-06-29 | Байер Кропсайенс Акциенгезельшафт | Двойные фунгицидные смеси |
BR112015012055B1 (pt) | 2012-11-30 | 2021-01-12 | Bayer Cropscience Ag | composição fungicida ternária, seu processo de preparação, método para controlar um ou mais microrganismos nocivos, semente resistente a microrganismos nocivos e seu método de tratamento |
UA116223C2 (uk) | 2012-11-30 | 2018-02-26 | Байєр Кропсайєнс Акцієнгезелльшафт | Подвійна фунгіцидна суміш |
BR112015012702A2 (pt) | 2012-12-03 | 2017-07-11 | Bayer Cropscience Ag | composição que compreende um agente de controle biológico e um fungicida |
CA2893080A1 (en) | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
CA2893185A1 (en) | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
EP2925145A2 (en) | 2012-12-03 | 2015-10-07 | Bayer CropScience AG | Composition comprising biological control agents |
CN105025722A (zh) | 2012-12-03 | 2015-11-04 | 拜耳作物科学股份公司 | 包含生物防治剂和杀虫剂的组合物 |
WO2014086753A2 (en) | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising biological control agents |
WO2014086758A2 (en) | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising a biological control agent and an insecticide |
BR112015012789A2 (pt) | 2012-12-03 | 2017-07-11 | Bayer Cropscience Ag | composição que compreende um agente de controle biológico e um inseticida |
WO2014090765A1 (en) | 2012-12-12 | 2014-06-19 | Bayer Cropscience Ag | Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops |
AR093996A1 (es) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | Combinaciones bactericidas y fungicidas binarias |
EP2935218A1 (en) | 2012-12-19 | 2015-10-28 | Bayer CropScience AG | Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides |
EP2953469A1 (en) | 2013-02-11 | 2015-12-16 | Bayer Cropscience LP | Compositions comprising a streptomyces-based biological control agent and another biological control agent |
JP2016507572A (ja) | 2013-02-11 | 2016-03-10 | バイエル クロップサイエンス エルピーBayer Cropscience Lp | ストレプトマイセス(Streptomyces)に基づく生物学的防除剤および殺虫剤を含む組成物 |
KR20150119023A (ko) | 2013-02-11 | 2015-10-23 | 바이엘 크롭사이언스 엘피 | 고제로틴 및 살진균제를 포함하는 조성물 |
EP2964767B1 (en) | 2013-03-07 | 2019-12-18 | BASF Agricultural Solutions Seed US LLC | Toxin genes and methods for their use |
BR112015026235A2 (pt) | 2013-04-19 | 2017-10-10 | Bayer Cropscience Ag | método para melhorar a utilização do potencial de produção de plantas transgênicas envolvendo a aplicação de um derivado de ftaldiamida |
WO2014170364A1 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Ag | Binary insecticidal or pesticidal mixture |
TW201507722A (zh) | 2013-04-30 | 2015-03-01 | Bayer Cropscience Ag | 做為殺線蟲劑及殺體內寄生蟲劑的n-(2-鹵素-2-苯乙基)-羧醯胺類 |
WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
WO2014206953A1 (en) | 2013-06-26 | 2014-12-31 | Bayer Cropscience Ag | N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives |
WO2015082586A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Ag | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
TW201607929A (zh) | 2013-12-05 | 2016-03-01 | 拜耳作物科學公司 | N-環烷基-n-{[2-(1-經取代環烷基)苯基]亞甲基}-(硫代)甲醯胺衍生物 |
EP2885970A1 (en) | 2013-12-21 | 2015-06-24 | Bayer CropScience AG | Fungicide compositions comprising compound I, at least one succinate dehydrogenase (SDH) inhibitor and at least one triazole fungicide |
CN106459986B (zh) | 2014-03-11 | 2020-06-30 | 拜耳作物科学有限合伙公司 | Hppd变体和使用方法 |
KR102308359B1 (ko) * | 2014-03-20 | 2021-10-06 | 몬산토 테크놀로지 엘엘씨 | 형질전환 옥수수 이벤트 mon 87419 및 이의 사용 방법 |
WO2015160620A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and an insecticide |
WO2015160618A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a biological control agent |
WO2015160619A1 (en) | 2014-04-16 | 2015-10-22 | Bayer Cropscience Lp | Compositions comprising ningnanmycin and a fungicide |
BR112017022000A2 (pt) | 2015-04-13 | 2018-07-03 | Bayer Cropscience Ag | derivados de n-cicloalquil-n-(biheterocicliletileno)-(tio)carboxamida. |
ES2951012T3 (es) * | 2015-05-14 | 2023-10-17 | Agrivida Inc | Producción de glucanasa y métodos de uso de la misma |
EP3097782A1 (en) | 2015-05-29 | 2016-11-30 | Bayer CropScience Aktiengesellschaft | Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents |
WO2017042259A1 (en) | 2015-09-11 | 2017-03-16 | Bayer Cropscience Aktiengesellschaft | Hppd variants and methods of use |
CA3206286A1 (en) | 2015-10-12 | 2017-04-20 | Pioneer Hi-Bred International, Inc. | Fungal entomopathogen biocides and their use in plants |
CN105494079A (zh) * | 2015-12-02 | 2016-04-20 | 中国农业科学院棉花研究所 | 一种获得高抗虫性棉花植株的方法 |
CN107090464B (zh) * | 2016-02-18 | 2020-06-23 | 中国种子集团有限公司 | 抗虫抗除草剂玉米转化事件及其创制方法和检测方法 |
WO2018019676A1 (en) | 2016-07-29 | 2018-02-01 | Bayer Cropscience Aktiengesellschaft | Active compound combinations and methods to protect the propagation material of plants |
BR112019010476A2 (pt) | 2016-11-23 | 2019-09-10 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, vetor, célula hospedeira, planta transgênica, semente transgênica, polipeptídeo recombinante, composição, método para controlar uma população de pragas, para matar pragas, para produzir um polipeptídeo, planta ou célula vegetal, método para proteger uma planta contra uma praga, para aumentar o rendimento em uma planta, uso e produto primário |
EP3555056A1 (en) | 2016-12-19 | 2019-10-23 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
UY37570A (es) | 2017-01-18 | 2018-08-31 | Bayer Cropscience Lp | Uso de bp005 para el control de patógenos de planta |
BR112019014727A2 (pt) | 2017-01-18 | 2020-04-07 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico, vector, célula, planta, semente, polipeptídeo, composição, métodos para o controle de uma população de pragas, para matar uma praga, para produzir um polipeptídeo, para proteger uma planta e para aumentar o rendimento em uma planta, uso do ácido nucleico e produto de base |
EP3585773B1 (en) | 2017-02-21 | 2021-04-07 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
BR112019018056A2 (pt) | 2017-03-07 | 2020-08-11 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos |
EP3606912A1 (en) | 2017-04-07 | 2020-02-12 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
WO2018188962A1 (en) | 2017-04-11 | 2018-10-18 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
WO2018195256A1 (en) | 2017-04-21 | 2018-10-25 | Bayer Cropscience Lp | Method of improving crop safety |
WO2018202491A1 (en) | 2017-05-04 | 2018-11-08 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
US20210084900A1 (en) | 2017-05-04 | 2021-03-25 | Basf Se | Substituted 5-(haloalkyl)-5-hydroxy-isoxazoles for Combating Phytopathogenic Fungi |
WO2018219797A1 (en) | 2017-06-02 | 2018-12-06 | Basf Se | Substituted oxadiazoles for combating phytopathogenic fungi |
EP3642187A1 (en) | 2017-06-19 | 2020-04-29 | Basf Se | 2-[[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]aryloxy](thio)acetamides for combating phytopathogenic fungi |
AU2018308563B2 (en) | 2017-07-27 | 2024-01-04 | Basf Se | Use of herbicidal compositions based on L-glufosinate in tolerant field crops |
WO2019025250A1 (en) | 2017-08-04 | 2019-02-07 | Basf Se | SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR COMBATING PHYTOPATHOGENIC FUNGI |
WO2019038042A1 (en) | 2017-08-21 | 2019-02-28 | Basf Se | SUBSTITUTED TRIFLUOROMETHYLOXADIAZOLES FOR THE CONTROL OF PHYTOPATHOGENIC FUNGI |
EP3684761A1 (en) | 2017-09-18 | 2020-07-29 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
WO2019068811A1 (en) | 2017-10-06 | 2019-04-11 | Bayer Aktiengesellschaft | COMPOSITIONS COMPRISING FLUOPYRAM AND TIOXAZAFENE |
US11279944B2 (en) | 2017-10-24 | 2022-03-22 | BASF Agricultural Solutions Seed US LLC | Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean |
WO2019083808A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS |
EP3713936B1 (en) | 2017-11-23 | 2021-10-20 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
WO2019121143A1 (en) | 2017-12-20 | 2019-06-27 | Basf Se | Substituted cyclopropyl derivatives |
WO2019137995A1 (en) | 2018-01-11 | 2019-07-18 | Basf Se | Novel pyridazine compounds for controlling invertebrate pests |
EP3740070A1 (en) | 2018-01-18 | 2020-11-25 | Pioneer Hi-Bred International Inc. | Alginate encapsulation of fungal microsclerotia |
WO2019145221A1 (en) | 2018-01-29 | 2019-08-01 | BASF Agro B.V. | New agrochemical formulations |
WO2019154665A1 (en) | 2018-02-07 | 2019-08-15 | Basf Se | New pyridine carboxamides |
WO2019154663A1 (en) | 2018-02-07 | 2019-08-15 | Basf Se | New pyridine carboxamides |
WO2019166257A1 (en) | 2018-03-01 | 2019-09-06 | BASF Agro B.V. | Fungicidal compositions of mefentrifluconazole |
AU2019261281A1 (en) | 2018-04-27 | 2020-10-01 | Pioneer Hi-Bred International, Inc. | Maize event DP-023211-2 and methods for detection thereof |
WO2019219464A1 (en) | 2018-05-15 | 2019-11-21 | Basf Se | Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi |
WO2019224092A1 (en) | 2018-05-22 | 2019-11-28 | Basf Se | Pesticidally active c15-derivatives of ginkgolides |
US20210323950A1 (en) | 2018-06-04 | 2021-10-21 | Bayer Aktiengesellschaft | Herbicidally active bicyclic benzoylpyrazoles |
EP3613736A1 (en) | 2018-08-22 | 2020-02-26 | Basf Se | Substituted glutarimide derivatives |
EP3628158A1 (en) | 2018-09-28 | 2020-04-01 | Basf Se | Pesticidal mixture comprising a mesoionic compound and a biopesticide |
MX2021004735A (es) | 2018-10-23 | 2021-06-04 | Basf Se | Compuestos plaguicidas triciclicos. |
EP3643705A1 (en) | 2018-10-24 | 2020-04-29 | Basf Se | Pesticidal compounds |
BR112021011370A2 (pt) | 2018-12-14 | 2021-08-31 | Pioneer Hi-Bred International, Inc. | Produtos biológicos e seu uso em plantas |
EP3670501A1 (en) | 2018-12-17 | 2020-06-24 | Basf Se | Substituted [1,2,4]triazole compounds as fungicides |
ES2950006T3 (es) | 2019-01-11 | 2023-10-04 | Basf Se | Formas cristalinas de 1-(1,2-dimetilpropil)-N-etil-5-metil-N-piridazin-4-il-pirazol-4-carboxamida |
EP3696177A1 (en) | 2019-02-12 | 2020-08-19 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
EP3965575A1 (en) | 2019-05-10 | 2022-03-16 | Bayer CropScience LP | Active compound combinations |
EP3769623A1 (en) | 2019-07-22 | 2021-01-27 | Basf Se | Mesoionic imidazolium compounds and derivatives for combating animal pests |
EP3975718A1 (en) | 2019-05-29 | 2022-04-06 | Basf Se | Mesoionic imidazolium compounds and derivatives for combating animal pests |
WO2020244969A1 (en) | 2019-06-06 | 2020-12-10 | Basf Se | Pyridine derivatives and their use as fungicides |
WO2020244970A1 (en) | 2019-06-06 | 2020-12-10 | Basf Se | New carbocyclic pyridine carboxamides |
WO2020244968A1 (en) | 2019-06-06 | 2020-12-10 | Basf Se | Fungicidal n-(pyrid-3-yl)carboxamides |
EP3766879A1 (en) | 2019-07-19 | 2021-01-20 | Basf Se | Pesticidal pyrazole derivatives |
CA3147858A1 (en) | 2019-07-22 | 2021-01-28 | Bayer Aktiengesellschaft | 5-amino substituted pyrazoles and triazoles as pest control agents |
CN114502545A (zh) | 2019-07-23 | 2022-05-13 | 拜耳公司 | 作为农药的新的杂芳基-三唑化合物 |
CA3148209A1 (en) | 2019-07-23 | 2021-01-28 | Bayer Aktiengesellschaft | Novel heteroaryl-triazole compounds as pesticides |
WO2021022069A1 (en) | 2019-08-01 | 2021-02-04 | Bayer Cropscience Lp | Method of improving cold stress tolerance and crop safety |
EP3701796A1 (en) | 2019-08-08 | 2020-09-02 | Bayer AG | Active compound combinations |
WO2021058659A1 (en) | 2019-09-26 | 2021-04-01 | Bayer Aktiengesellschaft | Rnai-mediated pest control |
WO2021063735A1 (en) | 2019-10-02 | 2021-04-08 | Basf Se | New bicyclic pyridine derivatives |
WO2021063736A1 (en) | 2019-10-02 | 2021-04-08 | Basf Se | Bicyclic pyridine derivatives |
MX2022003964A (es) | 2019-10-02 | 2022-04-25 | Bayer Ag | Combinaciones de compuestos activos que comprenden acidos grasos. |
CN114728928A (zh) | 2019-10-09 | 2022-07-08 | 拜耳公司 | 作为农药的新的杂芳基三唑化合物 |
AU2020363864A1 (en) | 2019-10-09 | 2022-04-21 | Bayer Aktiengesellschaft | Novel heteroaryl-triazole compounds as pesticides |
WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
KR20220098170A (ko) | 2019-11-07 | 2022-07-11 | 바이엘 악티엔게젤샤프트 | 동물 해충 방제를 위한 치환된 술포닐 아미드 |
WO2021097162A1 (en) | 2019-11-13 | 2021-05-20 | Bayer Cropscience Lp | Beneficial combinations with paenibacillus |
EP4061131A1 (en) | 2019-11-18 | 2022-09-28 | Bayer Aktiengesellschaft | Active compound combinations comprising fatty acids |
TW202134226A (zh) | 2019-11-18 | 2021-09-16 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基-三唑化合物 |
TW202136248A (zh) | 2019-11-25 | 2021-10-01 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基-三唑化合物 |
CN115335392A (zh) | 2020-01-31 | 2022-11-11 | 成对植物服务股份有限公司 | 植物避荫反应的抑制 |
CN115551839A (zh) | 2020-02-18 | 2022-12-30 | 拜耳公司 | 作为农药的杂芳基-三唑化合物 |
EP3708565A1 (en) | 2020-03-04 | 2020-09-16 | Bayer AG | Pyrimidinyloxyphenylamidines and the use thereof as fungicides |
MX2022012878A (es) | 2020-04-16 | 2022-12-08 | Pairwise Plants Services Inc | Metodos para controlar el tama?o del meristema para la mejora de cultivos. |
WO2021209490A1 (en) | 2020-04-16 | 2021-10-21 | Bayer Aktiengesellschaft | Cyclaminephenylaminoquinolines as fungicides |
MX2022013157A (es) | 2020-04-21 | 2022-11-16 | Bayer Ag | Derivados de heterociclos condensados sustituidos con 2-(het)arilo como plaguicidas. |
CA3179211A1 (en) | 2020-04-24 | 2021-10-28 | Monsanto Technology Llc | Transgenic corn event mon95275 and methods for detection and uses thereof |
EP3903583A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iii |
WO2021219513A1 (en) | 2020-04-28 | 2021-11-04 | Basf Se | Pesticidal compounds |
EP3903584A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iv |
EP3903582A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ii |
EP3903581A1 (en) | 2020-04-28 | 2021-11-03 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i |
BR112022022595A2 (pt) | 2020-05-06 | 2022-12-20 | Bayer Ag | Piridina (tio)amidas como compostos fungicidas |
TW202208347A (zh) | 2020-05-06 | 2022-03-01 | 德商拜耳廠股份有限公司 | 作為殺蟲劑之新穎雜芳基三唑化合物 |
JP2023525349A (ja) | 2020-05-12 | 2023-06-15 | バイエル、アクチエンゲゼルシャフト | 殺真菌性化合物としてのトリアジンおよびピリミジン(チオ)アミド化合物 |
EP3909950A1 (en) | 2020-05-13 | 2021-11-17 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
US20230192617A1 (en) | 2020-05-19 | 2023-06-22 | Bayer Cropscience Aktiengesellschaft | Azabicyclic(thio)amides as fungicidal compounds |
CN116096230A (zh) | 2020-06-02 | 2023-05-09 | 成对植物服务股份有限公司 | 控制分生组织大小以改良作物的方法 |
US20230278994A1 (en) | 2020-06-04 | 2023-09-07 | Bayer Aktiengesellschaft | Heterocyclyl pyrimidines and triazines as novel fungicides |
EP3945089A1 (en) | 2020-07-31 | 2022-02-02 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors v |
WO2021249800A1 (en) | 2020-06-10 | 2021-12-16 | Basf Se | Substituted [1,2,4]triazole compounds as fungicides |
US20230234945A1 (en) | 2020-06-10 | 2023-07-27 | Bayer Aktiengesellschaft | Azabicyclyl-substituted heterocycles as fungicides |
EP4168558A1 (en) | 2020-06-17 | 2023-04-26 | Pairwise Plants Services, Inc. | Methods for controlling meristem size for crop improvement |
CA3187296A1 (en) | 2020-06-18 | 2021-12-23 | Bayer Aktiengesellschaft | 3-(pyridazin-4-yl)-5,6-dihydro-4h-1,2,4-oxadiazine derivatives as fungicides for crop protection |
BR112022025344A2 (pt) | 2020-06-18 | 2023-01-03 | Bayer Ag | Composição para uso na agricultura |
WO2021255091A1 (en) | 2020-06-19 | 2021-12-23 | Bayer Aktiengesellschaft | 1,3,4-oxadiazoles and their derivatives as fungicides |
UY39275A (es) | 2020-06-19 | 2022-01-31 | Bayer Ag | 1,3,4-oxadiazol pirimidinas como fungicidas, procesos e intermediarios para su preparación, métodos de uso y usos de los mismos |
BR112022025710A2 (pt) | 2020-06-19 | 2023-03-07 | Bayer Ag | 1,3,4-oxadiazol pirimidinas e 1,3,4-oxadiazol piridinas como fungicidas |
UY39276A (es) | 2020-06-19 | 2022-01-31 | Bayer Ag | Uso de compuestos de 1,3,4–oxadiazol–2–ilpirimidina para controlar microorganismos fitopatógenos, métodos de uso y composiciones. |
EP3929189A1 (en) | 2020-06-25 | 2021-12-29 | Bayer Animal Health GmbH | Novel heteroaryl-substituted pyrazine derivatives as pesticides |
KR20230039665A (ko) | 2020-07-02 | 2023-03-21 | 바이엘 악티엔게젤샤프트 | 해충 방제제로서의 헤테로사이클 유도체 |
EP3939961A1 (en) | 2020-07-16 | 2022-01-19 | Basf Se | Strobilurin type compounds and their use for combating phytopathogenic fungi |
WO2022017836A1 (en) | 2020-07-20 | 2022-01-27 | BASF Agro B.V. | Fungicidal compositions comprising (r)-2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1- (1,2,4-triazol-1-yl)propan-2-ol |
CA3188278A1 (en) * | 2020-07-31 | 2022-02-03 | Inari Agriculture Technology, Inc. | Inht31 transgenic soybean |
US11242534B1 (en) | 2020-07-31 | 2022-02-08 | Inari Agriculture Technology, Inc. | INHT31 transgenic soybean |
US11326177B2 (en) | 2020-07-31 | 2022-05-10 | Inari Agriculture Technology, Inc. | INIR12 transgenic maize |
US20240011042A1 (en) | 2020-07-31 | 2024-01-11 | Inari Agriculture Technology, Inc. | Excisable plant transgenic loci with signature protospacer adjacent motifs or signature guide rna recognition sites |
US11369073B2 (en) | 2020-07-31 | 2022-06-28 | Inari Agriculture Technology, Inc. | INIR12 transgenic maize |
US11214811B1 (en) * | 2020-07-31 | 2022-01-04 | Inari Agriculture Technology, Inc. | INIR6 transgenic maize |
EP3970494A1 (en) | 2020-09-21 | 2022-03-23 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors viii |
WO2022033991A1 (de) | 2020-08-13 | 2022-02-17 | Bayer Aktiengesellschaft | 5-amino substituierte triazole als schädlingsbekämpfungsmittel |
WO2022053453A1 (de) | 2020-09-09 | 2022-03-17 | Bayer Aktiengesellschaft | Azolcarboxamide als schädlingsbekämpfungsmittel |
WO2022058327A1 (en) | 2020-09-15 | 2022-03-24 | Bayer Aktiengesellschaft | Substituted ureas and derivatives as new antifungal agents |
EP3974414A1 (de) | 2020-09-25 | 2022-03-30 | Bayer AG | 5-amino substituierte pyrazole und triazole als schädlingsbekämpfungsmittel |
CN116209355A (zh) | 2020-10-27 | 2023-06-02 | 巴斯夫农业公司 | 包含氯氟醚菌唑的组合物 |
WO2022090069A1 (en) | 2020-11-02 | 2022-05-05 | Basf Se | Compositions comprising mefenpyr-diethyl |
WO2022090071A1 (en) | 2020-11-02 | 2022-05-05 | Basf Se | Use of mefenpyr-diethyl for controlling phytopathogenic fungi |
WO2022106304A1 (en) | 2020-11-23 | 2022-05-27 | BASF Agro B.V. | Compositions comprising mefentrifluconazole |
EP4259627A1 (en) | 2020-12-14 | 2023-10-18 | Basf Se | Sulfoximine pesticides |
EP3915971A1 (en) | 2020-12-16 | 2021-12-01 | Bayer Aktiengesellschaft | Phenyl-s(o)n-phenylamidines and the use thereof as fungicides |
WO2022129188A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | 1,2,4-oxadiazol-3-yl pyrimidines as fungicides |
WO2022129196A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | Heterobicycle substituted 1,2,4-oxadiazoles as fungicides |
WO2022129190A1 (en) | 2020-12-18 | 2022-06-23 | Bayer Aktiengesellschaft | (hetero)aryl substituted 1,2,4-oxadiazoles as fungicides |
JP2023554063A (ja) | 2020-12-18 | 2023-12-26 | バイエル・アクチエンゲゼルシヤフト | 作物における耐性植物病原性真菌を防除するためのdhodh阻害剤の使用 |
EP4036083A1 (de) | 2021-02-02 | 2022-08-03 | Bayer Aktiengesellschaft | 5-oxy substituierte hetereozyklen, als schädlingsbekämpfungsmittel |
EP4043444A1 (en) | 2021-02-11 | 2022-08-17 | Basf Se | Substituted isoxazoline derivatives |
BR112023015909A2 (pt) | 2021-02-11 | 2023-11-21 | Monsanto Technology Llc | Métodos e composições para modificar níveis de citocinina oxidase em plantas |
CA3211121A1 (en) | 2021-02-25 | 2022-09-01 | Pairwise Plants Services, Inc. | Methods and compositions for modifying root architecture in plants |
BR112023019788A2 (pt) | 2021-03-30 | 2023-11-07 | Bayer Ag | 3-(hetero)aril-5-clorodifluorometil-1,2,4-oxadiazol como fungicida |
BR112023019400A2 (pt) | 2021-03-30 | 2023-12-05 | Bayer Ag | 3-(hetero)aril-5-clorodifluorometil-1,2,4-oxadiazol como fungicida |
IL308130A (en) | 2021-05-03 | 2023-12-01 | Basf Se | Additives to improve the effectiveness of pesticides of exterminating microorganisms |
BR112023022763A2 (pt) | 2021-05-06 | 2024-01-02 | Bayer Ag | Imidazóis anulados substituídos por alquilamida e uso dos mesmos como inseticidas |
CN117651702A (zh) | 2021-05-12 | 2024-03-05 | 拜耳公司 | 作为害虫防治剂的2-(杂)芳基取代的稠合杂环衍生物 |
EP4091451A1 (en) | 2021-05-17 | 2022-11-23 | BASF Agro B.V. | Compositions comprising mefentrifluconazole |
EP4341257A1 (en) | 2021-05-18 | 2024-03-27 | Basf Se | New substituted quinolines as fungicides |
BR112023024005A2 (pt) | 2021-05-18 | 2024-01-30 | Basf Se | Compostos, composição e processo para preparar compostos de fórmula i |
KR20240008857A (ko) | 2021-05-18 | 2024-01-19 | 바스프 에스이 | 살진균제로서의 신규한 치환된 피리딘 |
CN117897050A (zh) | 2021-06-17 | 2024-04-16 | 成对植物服务股份有限公司 | 大豆中生长调节因子家族转录因子的修饰 |
UY39827A (es) | 2021-06-24 | 2023-01-31 | Pairwise Plants Services Inc | Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento |
US20230027468A1 (en) | 2021-07-01 | 2023-01-26 | Pairwise Plants Services, Inc. | Methods and compositions for enhancing root system development |
EP4119547A1 (en) | 2021-07-12 | 2023-01-18 | Basf Se | Triazole compounds for the control of invertebrate pests |
CN117794907A (zh) | 2021-08-02 | 2024-03-29 | 巴斯夫欧洲公司 | (3-吡啶基)-喹唑啉 |
CN117794908A (zh) | 2021-08-02 | 2024-03-29 | 巴斯夫欧洲公司 | (3-喹啉基)-喹唑啉 |
WO2023019188A1 (en) | 2021-08-12 | 2023-02-16 | Pairwise Plants Services, Inc. | Modification of brassinosteroid receptor genes to improve yield traits |
WO2023017120A1 (en) | 2021-08-13 | 2023-02-16 | Bayer Aktiengesellschaft | Active compound combinations and fungicide compositions comprising those |
CA3229224A1 (en) | 2021-08-17 | 2023-02-23 | Pairwise Plants Services, Inc. | Methods and compositions for modifying cytokinin receptor histidine kinase genes in plants |
EP4140986A1 (en) | 2021-08-23 | 2023-03-01 | Basf Se | Pyrazine compounds for the control of invertebrate pests |
AU2022335669A1 (en) | 2021-08-25 | 2024-02-01 | Bayer Aktiengesellschaft | Novel pyrazinyl-triazole compounds as pesticides |
EP4140995A1 (en) | 2021-08-27 | 2023-03-01 | Basf Se | Pyrazine compounds for the control of invertebrate pests |
CA3230167A1 (en) | 2021-08-30 | 2023-03-09 | Pairwise Plants Services, Inc. | Modification of ubiquitin binding peptidase genes in plants for yield trait improvement |
EP4144739A1 (de) | 2021-09-02 | 2023-03-08 | Bayer Aktiengesellschaft | Anellierte pyrazole als schädlingsbekämpfungsmittel |
AR126938A1 (es) | 2021-09-02 | 2023-11-29 | Pairwise Plants Services Inc | Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento |
EP4151631A1 (en) | 2021-09-20 | 2023-03-22 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
AU2022352997A1 (en) | 2021-09-21 | 2024-04-04 | Pairwise Plants Services, Inc. | Methods and compositions for reducing pod shatter in canola |
WO2023060028A1 (en) | 2021-10-04 | 2023-04-13 | Pairwise Plants Services, Inc. | Methods for improving floret fertility and seed yield |
WO2023060152A2 (en) | 2021-10-07 | 2023-04-13 | Pairwise Plants Services, Inc. | Methods for improving floret fertility and seed yield |
WO2023072670A1 (en) | 2021-10-28 | 2023-05-04 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors x |
WO2023072671A1 (en) | 2021-10-28 | 2023-05-04 | Basf Se | Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ix |
WO2023078915A1 (en) | 2021-11-03 | 2023-05-11 | Bayer Aktiengesellschaft | Bis(hetero)aryl thioether (thio)amides as fungicidal compounds |
WO2023099445A1 (en) | 2021-11-30 | 2023-06-08 | Bayer Aktiengesellschaft | Bis(hetero)aryl thioether oxadiazines as fungicidal compounds |
EP4194453A1 (en) | 2021-12-08 | 2023-06-14 | Basf Se | Pyrazine compounds for the control of invertebrate pests |
AR127904A1 (es) | 2021-12-09 | 2024-03-06 | Pairwise Plants Services Inc | Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas |
EP4198033A1 (en) | 2021-12-14 | 2023-06-21 | Basf Se | Heterocyclic compounds for the control of invertebrate pests |
EP4198023A1 (en) | 2021-12-16 | 2023-06-21 | Basf Se | Pesticidally active thiosemicarbazone compounds |
WO2023147526A1 (en) | 2022-01-31 | 2023-08-03 | Pairwise Plants Services, Inc. | Suppression of shade avoidance response in plants |
WO2023148030A1 (en) | 2022-02-01 | 2023-08-10 | Globachem Nv | Methods and compositions for controlling pests in corn |
WO2023148028A1 (en) | 2022-02-01 | 2023-08-10 | Globachem Nv | Methods and compositions for controlling pests |
WO2023156402A1 (en) | 2022-02-17 | 2023-08-24 | Basf Se | Pesticidally active thiosemicarbazone compounds |
EP4238971A1 (en) | 2022-03-02 | 2023-09-06 | Basf Se | Substituted isoxazoline derivatives |
WO2023168217A1 (en) | 2022-03-02 | 2023-09-07 | Pairwise Plants Services, Inc. | Modification of brassinosteroid receptor genes to improve yield traits |
WO2023192838A1 (en) | 2022-03-31 | 2023-10-05 | Pairwise Plants Services, Inc. | Early flowering rosaceae plants with improved characteristics |
WO2023196886A1 (en) | 2022-04-07 | 2023-10-12 | Pairwise Plants Services, Inc. | Methods and compositions for improving resistance to fusarium head blight |
US20230383305A1 (en) | 2022-04-21 | 2023-11-30 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield traits |
US20230348922A1 (en) | 2022-05-02 | 2023-11-02 | Pairwise Plants Services, Inc. | Methods and compositions for enhancing yield and disease resistance |
WO2023213670A1 (en) | 2022-05-03 | 2023-11-09 | Bayer Aktiengesellschaft | Crystalline forms of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine |
WO2023213626A1 (en) | 2022-05-03 | 2023-11-09 | Bayer Aktiengesellschaft | Use of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine for controlling unwanted microorganisms |
US20230416767A1 (en) | 2022-05-05 | 2023-12-28 | Pairwise Plants Services, Inc. | Methods and compositions for modifying root architecture and/or improving plant yield traits |
CN114736922B (zh) * | 2022-05-20 | 2024-02-13 | 中国农业科学院生物技术研究所 | 一种耐草铵膦转基因玉米BrmB01的制备与检测方法及其应用 |
WO2024006679A1 (en) | 2022-06-27 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for modifying shade avoidance in plants |
US20240000031A1 (en) | 2022-06-29 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
WO2024006792A1 (en) | 2022-06-29 | 2024-01-04 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
WO2024028243A1 (en) | 2022-08-02 | 2024-02-08 | Basf Se | Pyrazolo pesticidal compounds |
WO2024030984A1 (en) | 2022-08-04 | 2024-02-08 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield traits |
US20240060081A1 (en) | 2022-08-11 | 2024-02-22 | Pairwise Plants Services, Inc. | Methods and compositions for controlling meristem size for crop improvement |
US20240090466A1 (en) | 2022-09-08 | 2024-03-21 | Pairwise Plants Services, Inc. | Methods and compositions for improving yield characteristics in plants |
EP4342885A1 (en) | 2022-09-20 | 2024-03-27 | Basf Se | N-(3-(aminomethyl)-phenyl)-5-(4-phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-amine derivatives and similar compounds as pesticides |
CN116144818B (zh) * | 2022-09-23 | 2023-11-07 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp026-2及其检测方法 |
WO2024068517A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
EP4295688A1 (en) | 2022-09-28 | 2023-12-27 | Bayer Aktiengesellschaft | Active compound combination |
WO2024068518A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-heteroaryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
WO2024068519A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
WO2024068520A1 (en) | 2022-09-28 | 2024-04-04 | Bayer Aktiengesellschaft | 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999032642A2 (en) * | 1997-12-22 | 1999-07-01 | Dekalb Genetics Corporation | Method for reduction of transgene copy number |
WO2000031268A1 (en) * | 1998-11-23 | 2000-06-02 | Pioneer Hi-Bred International, Inc. | Maize rad23 genes and uses thereof |
CN1305532A (zh) * | 1998-06-11 | 2001-07-25 | 纳幕尔杜邦公司 | 用于改变玉米脂质特征的去饱和酶的基因 |
US20030033632A1 (en) * | 2000-10-25 | 2003-02-13 | Pioneer Hi-Bred International, Inc. | Maize proteinase inhibitor-like polynucleotides and methods of use |
WO2004090143A2 (en) * | 2003-04-04 | 2004-10-21 | Pioneer Hi-Bred International, Inc. | Modulation of cytokinin activity in plants |
WO2004099447A2 (en) * | 2003-05-02 | 2004-11-18 | Dow Agrosciences Llc | Corn event tc1507 and methods for detection thereof |
CN1954072A (zh) * | 2004-03-08 | 2007-04-25 | 辛根塔参与股份公司 | 自加工的植物和植物部分 |
US20110154523A1 (en) * | 2009-12-17 | 2011-06-23 | Pioneer Hi-Bred International, Inc. | Maize event DP-004114-3 and methods for detection thereof |
CN104411828A (zh) * | 2012-04-24 | 2015-03-11 | 先锋国际良种公司 | 玉米事件dp-004114-3及其检测方法 |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US6083499A (en) | 1996-04-19 | 2000-07-04 | Mycogen Corporation | Pesticidal toxins |
US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
ATE327333T1 (de) | 1998-10-23 | 2006-06-15 | Mycogen Corp | Für 15kda und 45kda pestizid-proteine kodierende pflanzen-optimierte polynukleotide |
US6222106B1 (en) * | 1999-02-23 | 2001-04-24 | Dekalb Genetics Group | Inbred corn plant 01IUL6 and seeds thereof |
AR025349A1 (es) | 1999-08-23 | 2002-11-20 | Mycogen Corp | Metodos para controlar las plagas del gusano gris |
US6395485B1 (en) | 2000-01-11 | 2002-05-28 | Aventis Cropscience N.V. | Methods and kits for identifying elite event GAT-ZM1 in biological samples |
US20070277258A1 (en) * | 2000-07-19 | 2007-11-29 | Fisher Dane K | Nucleic acid molecules and other molecules associated with plants |
USH2191H1 (en) * | 2000-10-24 | 2007-06-05 | Snp Consortium | Identification and mapping of single nucleotide polymorphisms in the human genome |
US7892730B2 (en) * | 2000-12-22 | 2011-02-22 | Sagres Discovery, Inc. | Compositions and methods for cancer |
US6849790B2 (en) * | 2001-03-15 | 2005-02-01 | Advanta Technology, Ltd. | Inbred corn line G3601 |
US6828492B2 (en) * | 2001-03-15 | 2004-12-07 | Advanta Technology Ltd | Inbred corn line G4901 |
US7129042B2 (en) * | 2003-11-03 | 2006-10-31 | Diagnostic Hybrids, Inc. | Compositions and methods for detecting severe acute respiratory syndrome coronavirus |
US7157281B2 (en) * | 2003-12-11 | 2007-01-02 | Monsanto Technology Llc | High lysine maize compositions and event LY038 maize plants |
UA97088C2 (ru) * | 2004-09-29 | 2012-01-10 | Пионер Хай-Бред Интернешнл, Инк. | Трансгенная кукуруза das-59122-7, стойкая к насекомым, и способы его обнаружения |
WO2006053147A1 (en) | 2004-11-12 | 2006-05-18 | Pioneer Hi-Bred International, Inc. | Method of evaluating plant protection |
EP2094831B1 (en) * | 2006-12-07 | 2015-09-16 | Dow AgroSciences LLC | Novel selectable marker genes |
MX2009005285A (es) * | 2006-12-22 | 2009-05-28 | Pioneer Hi Bred Int | Estrategias de manejo de resistencia. |
WO2009046291A2 (en) * | 2007-10-04 | 2009-04-09 | Pioneer Hi-Bred International, Inc. | Laboratory-selected colonies of western corn rootworm with increased tolerance to maize containing event das-59122-7 and methods of use |
WO2009088732A1 (en) | 2008-01-10 | 2009-07-16 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis gene with coleopteran activity |
US8273535B2 (en) | 2008-02-08 | 2012-09-25 | Dow Agrosciences, Llc | Methods for detection of corn event DAS-59132 |
WO2015073555A1 (en) * | 2013-11-13 | 2015-05-21 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
TWI713613B (zh) * | 2015-11-04 | 2020-12-21 | 美商利安德化學科技有限公司 | 用於獲得分支鏈醛之烯烴加氫甲醯化方法 |
-
2010
- 2010-12-16 MX MX2012006936A patent/MX2012006936A/es active IP Right Grant
- 2010-12-16 CN CN201080057507.0A patent/CN102892284B/zh active Active
- 2010-12-16 CN CN201610113418.2A patent/CN106047918B/zh active Active
- 2010-12-16 EP EP10803313.5A patent/EP2512226B1/en active Active
- 2010-12-16 CN CN202110310883.6A patent/CN113373174A/zh active Pending
- 2010-12-16 CA CA2784106A patent/CA2784106C/en active Active
- 2010-12-16 US US12/970,052 patent/US8575434B2/en active Active
- 2010-12-16 WO PCT/US2010/060818 patent/WO2011084621A1/en active Application Filing
- 2010-12-16 PL PL10803313T patent/PL2512226T3/pl unknown
- 2010-12-16 ES ES10803313T patent/ES2730684T3/es active Active
- 2010-12-16 HU HUE10803313 patent/HUE045002T2/hu unknown
- 2010-12-17 AR ARP100104748A patent/AR087225A1/es active IP Right Grant
-
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- 2013-10-15 US US14/054,409 patent/US9790561B2/en active Active
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Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999032642A2 (en) * | 1997-12-22 | 1999-07-01 | Dekalb Genetics Corporation | Method for reduction of transgene copy number |
CN1305532A (zh) * | 1998-06-11 | 2001-07-25 | 纳幕尔杜邦公司 | 用于改变玉米脂质特征的去饱和酶的基因 |
WO2000031268A1 (en) * | 1998-11-23 | 2000-06-02 | Pioneer Hi-Bred International, Inc. | Maize rad23 genes and uses thereof |
US20030033632A1 (en) * | 2000-10-25 | 2003-02-13 | Pioneer Hi-Bred International, Inc. | Maize proteinase inhibitor-like polynucleotides and methods of use |
WO2004090143A2 (en) * | 2003-04-04 | 2004-10-21 | Pioneer Hi-Bred International, Inc. | Modulation of cytokinin activity in plants |
WO2004099447A2 (en) * | 2003-05-02 | 2004-11-18 | Dow Agrosciences Llc | Corn event tc1507 and methods for detection thereof |
CN1954072A (zh) * | 2004-03-08 | 2007-04-25 | 辛根塔参与股份公司 | 自加工的植物和植物部分 |
US20110154523A1 (en) * | 2009-12-17 | 2011-06-23 | Pioneer Hi-Bred International, Inc. | Maize event DP-004114-3 and methods for detection thereof |
CN102892284A (zh) * | 2009-12-17 | 2013-01-23 | 先锋国际良种公司 | 玉米事件dp-004114-3及其检测方法 |
CN106047918A (zh) * | 2009-12-17 | 2016-10-26 | 先锋国际良种公司 | 玉米事件dp‑004114‑3及其检测方法 |
CN104411828A (zh) * | 2012-04-24 | 2015-03-11 | 先锋国际良种公司 | 玉米事件dp-004114-3及其检测方法 |
CN109468314A (zh) * | 2012-04-24 | 2019-03-15 | 先锋国际良种公司 | 玉米事件dp-004114-3及其检测方法 |
Non-Patent Citations (1)
Title |
---|
张建福;谢华安;王国英;SWAPAN K.DATTA;: "玉米PEPC基因和PPDK基因在籼稻明恢63中的整合及与光合作用相关的特性分析", 分子植物育种, no. 05, 28 September 2006 (2006-09-28) * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116640761A (zh) * | 2023-07-20 | 2023-08-25 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp018-1及其检测方法 |
CN116640761B (zh) * | 2023-07-20 | 2023-09-22 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp018-1及其检测方法 |
CN117144054A (zh) * | 2023-10-27 | 2023-12-01 | 莱肯生物科技(海南)有限公司 | 一种核酸检测方法及其应用 |
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WO2011084621A1 (en) | 2011-07-14 |
US11155883B2 (en) | 2021-10-26 |
CN106047918A (zh) | 2016-10-26 |
US20110154523A1 (en) | 2011-06-23 |
US20150093747A1 (en) | 2015-04-02 |
CA2784106C (en) | 2020-03-24 |
CN102892284A (zh) | 2013-01-23 |
CA2784106A1 (en) | 2011-07-14 |
ES2730684T3 (es) | 2019-11-12 |
HUE045002T2 (hu) | 2019-11-28 |
US20180023148A1 (en) | 2018-01-25 |
US9790561B2 (en) | 2017-10-17 |
PL2512226T3 (pl) | 2019-10-31 |
US20190136331A1 (en) | 2019-05-09 |
AR087225A1 (es) | 2014-03-12 |
US10190179B2 (en) | 2019-01-29 |
CN106047918B (zh) | 2021-04-09 |
US20220002821A1 (en) | 2022-01-06 |
CN102892284B (zh) | 2016-03-23 |
BR112012014665A2 (pt) | 2015-08-25 |
MX2012006936A (es) | 2012-07-17 |
EP2512226B1 (en) | 2019-05-01 |
US8575434B2 (en) | 2013-11-05 |
EP2512226A1 (en) | 2012-10-24 |
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