FR2481318A1 - Procede d'analyse immunologique - Google Patents
Procede d'analyse immunologique Download PDFInfo
- Publication number
- FR2481318A1 FR2481318A1 FR8108105A FR8108105A FR2481318A1 FR 2481318 A1 FR2481318 A1 FR 2481318A1 FR 8108105 A FR8108105 A FR 8108105A FR 8108105 A FR8108105 A FR 8108105A FR 2481318 A1 FR2481318 A1 FR 2481318A1
- Authority
- FR
- France
- Prior art keywords
- cea
- substance
- enzyme
- reagent
- active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004458 analytical method Methods 0.000 title claims abstract description 23
- 230000001900 immune effect Effects 0.000 title claims description 19
- 238000000034 method Methods 0.000 claims abstract description 35
- 239000000126 substance Substances 0.000 claims abstract description 33
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
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- 239000012042 active reagent Substances 0.000 claims abstract description 14
- 230000008105 immune reaction Effects 0.000 claims abstract description 13
- 238000011534 incubation Methods 0.000 claims abstract description 12
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- 239000007790 solid phase Substances 0.000 claims abstract description 12
- 238000000926 separation method Methods 0.000 claims abstract description 6
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- 238000004451 qualitative analysis Methods 0.000 claims abstract description 5
- 239000000427 antigen Substances 0.000 claims description 14
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- 239000012491 analyte Substances 0.000 claims description 8
- 241000283707 Capra Species 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 2
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- 239000013543 active substance Substances 0.000 claims 1
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- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
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- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- RKUNBYITZUJHSG-UHFFFAOYSA-N Hyosciamin-hydrochlorid Natural products CN1C(C2)CCC1CC2OC(=O)C(CO)C1=CC=CC=C1 RKUNBYITZUJHSG-UHFFFAOYSA-N 0.000 description 1
- 229920006370 Kynar Polymers 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
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- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
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- 101100450563 Mus musculus Serpind1 gene Proteins 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
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- 229930182558 Sterol Natural products 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
- 229960004373 acetylcholine Drugs 0.000 description 1
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- XMLNCADGRIEXPK-KUMOIWDRSA-M chembl2146143 Chemical compound [Br-].O([C@H]1C[C@H]2CC[C@@H](C1)[N+]2(C)C)C(=O)C(CO)C1=CC=CC=C1 XMLNCADGRIEXPK-KUMOIWDRSA-M 0.000 description 1
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 125000002573 ethenylidene group Chemical group [*]=C=C([H])[H] 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229920000592 inorganic polymer Polymers 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
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- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
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- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
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- 238000002203 pretreatment Methods 0.000 description 1
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Reproductive Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH320980A CH642458A5 (en) | 1980-04-25 | 1980-04-25 | Immunological method |
| CH589880A CH651396A5 (en) | 1980-08-04 | 1980-08-04 | Immunological method for detecting and determining carcinoembryonic antigen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2481318A1 true FR2481318A1 (fr) | 1981-10-30 |
| FR2481318B1 FR2481318B1 (enrdf_load_stackoverflow) | 1984-10-19 |
Family
ID=25692464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8108105A Granted FR2481318A1 (fr) | 1980-04-25 | 1981-04-23 | Procede d'analyse immunologique |
Country Status (11)
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2487983A1 (fr) * | 1980-08-04 | 1982-02-05 | Hybritech Inc | Procede d'analyse immunometrique |
| US5011771A (en) * | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
| FR2710410A1 (fr) * | 1993-09-20 | 1995-03-31 | Bio Merieux | Procédé et dispositif pour la détermination d'un analyte dans un échantillon . |
| US5422239A (en) * | 1980-09-19 | 1995-06-06 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6406920B1 (en) | 1980-06-20 | 2002-06-18 | Inverness Medical Switzerland Gmbh | Processes and apparatus for carrying out specific binding assays |
| EP0111762B1 (en) * | 1980-06-20 | 1987-11-19 | Unilever Plc | Processes and apparatus for carrying out specific binding assays |
| EP0044219A1 (en) * | 1980-07-16 | 1982-01-20 | Unilever Plc | Methods of immuno analysis using monoclonal antibodies |
| EP0049898B2 (en) * | 1980-10-15 | 1991-08-14 | Takeda Chemical Industries, Ltd. | Method for immunochemical assay and kit therefor |
| US4837167A (en) * | 1981-01-30 | 1989-06-06 | Centocor, Inc. | Immunoassay for multi-determinant antigens using high-affinity |
| GB2118300B (en) * | 1982-02-12 | 1985-06-19 | Corning Glass Works | Method of immunoassay |
| DK76983A (da) * | 1982-03-05 | 1983-09-06 | Takeda Chemical Industries Ltd | Fremgangsmaade og reagens til immunkemisk bestemmelse af humant choriogonadotropin |
| DE3225027A1 (de) * | 1982-07-05 | 1984-01-05 | Boehringer Mannheim Gmbh, 6800 Mannheim | Immunchemisches messverfahren |
| IL73938A (en) * | 1984-01-02 | 1989-09-28 | Boehringer Mannheim Gmbh | Process and reagent for the determination of polyvalent antigen by incubation with three different receptors |
| US4690890A (en) * | 1984-04-04 | 1987-09-01 | Cetus Corporation | Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay |
| US4935339A (en) * | 1985-05-07 | 1990-06-19 | Nichols Institute Diagnostics | Delayed solid phase immunologic assay |
| US5770459A (en) * | 1986-04-30 | 1998-06-23 | Igen International, Inc. | Methods and apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence detection |
| DE3638767A1 (de) * | 1986-11-13 | 1988-05-26 | Behringwerke Ag | Laktoferrin enthaltendes inkubationsmedium fuer festphasenimmunometrisches verfahren und seine verwendung |
| US5935779A (en) * | 1988-11-03 | 1999-08-10 | Igen International Inc. | Methods for improved particle electrochemiluminescence assay |
| US6881589B1 (en) | 1987-04-30 | 2005-04-19 | Bioveris Corporation | Electrochemiluminescent localizable complexes for assay compositions |
| WO1989002078A1 (en) * | 1987-08-25 | 1989-03-09 | Fuji Yakuhin Kogyo Kabushiki Kaisha | Method for diagnosing chronic articular rheumatism |
| KR920002182B1 (ko) * | 1987-10-30 | 1992-03-19 | 후지야쿠힝 고오교오 가부시끼가이샤 | 간장암질환의 진단법 |
| DE3807440A1 (de) * | 1988-03-07 | 1989-09-21 | Progen Biotechnik Gmbh | Verfahren zum immunologischen nachweis von substanzen sowie eine derartige zusammensetzung und ein testkit |
| US5962218A (en) * | 1988-11-03 | 1999-10-05 | Igen International Inc. | Methods and apparatus for improved luminescence assays |
| US5798083A (en) * | 1988-11-03 | 1998-08-25 | Igen International, Inc. | Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection |
| US5779976A (en) * | 1988-11-03 | 1998-07-14 | Igen International, Inc. | Apparatus for improved luminescence assays |
| US5746974A (en) * | 1988-11-03 | 1998-05-05 | Igen International, Inc. | Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence and chemiluminescence detection |
| US5705402A (en) * | 1988-11-03 | 1998-01-06 | Igen International, Inc. | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets |
| CA2005204C (en) * | 1988-12-12 | 2002-08-06 | John Cooper Cox | Solid phase immunoassay with lyophilised conjugate |
| US5176999A (en) * | 1989-12-07 | 1993-01-05 | Eastman Kodak Company | Buffered wash composition, insolubilizing composition, test kits and method of use |
| ZA92803B (en) | 1991-02-06 | 1992-11-25 | Igen Inc | Method and apparatus for magnetic microparticulate based luminescene asay including plurality of magnets |
| US6201109B1 (en) | 1993-01-13 | 2001-03-13 | Dade Behring Marburg Gmbh | Assay for bone alkaline phosphatase |
| JP3694894B2 (ja) * | 1994-02-19 | 2005-09-14 | 生化学工業株式会社 | 正常アグリカンの測定法および測定用キット |
| EP1030179B1 (en) | 1998-09-01 | 2005-11-09 | Taiho Pharmaceutical Company Limited | Method for assaying human thymidylate synthase and assay kit |
| ATE365326T1 (de) | 1999-04-12 | 2007-07-15 | Sumitomo Chemical Co | Verfahren zur analyse der menge an intraabdominalem fettgewebe |
| EP1893990B1 (en) | 2005-06-03 | 2018-05-02 | Board of Regents of the University of Texas System | Electrochemistry and electrogenerated chemiluminescence with a single faradaic electrode |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1572220A (en) * | 1976-10-07 | 1980-07-30 | Mochida Pharm Co Ltd | Immunochemical process of measuring physiologically active substances |
| GB1549069A (en) * | 1976-12-10 | 1979-08-01 | Erba Farmitalia | Enzyme linked immunoassay |
| US4244940A (en) * | 1978-09-05 | 1981-01-13 | Bio-Rad Laboratories, Inc. | Single-incubation two-site immunoassay |
| EP0042755B1 (en) * | 1980-06-20 | 1988-08-24 | Unilever Plc | Processes and apparatus for carrying out specific binding assays |
-
1981
- 1981-03-23 CA CA000373607A patent/CA1160566A/en not_active Expired
- 1981-04-06 DK DK155881A patent/DK151399C/da not_active IP Right Cessation
- 1981-04-13 IT IT21122/81A patent/IT1137344B/it active
- 1981-04-14 DE DE3115115A patent/DE3115115C2/de not_active Expired
- 1981-04-15 NL NLAANVRAGE8101860,A patent/NL187545B/xx not_active Application Discontinuation
- 1981-04-22 SE SE8102558A patent/SE460930B/sv not_active IP Right Cessation
- 1981-04-23 FR FR8108105A patent/FR2481318A1/fr active Granted
- 1981-04-24 AT AT0186481A patent/AT373398B/de not_active IP Right Cessation
- 1981-04-24 GB GB8112730A patent/GB2074727B/en not_active Expired
- 1981-04-24 AU AU69811/81A patent/AU542563B2/en not_active Expired
- 1981-04-24 NO NO811407A patent/NO159620C/no not_active IP Right Cessation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2487983A1 (fr) * | 1980-08-04 | 1982-02-05 | Hybritech Inc | Procede d'analyse immunometrique |
| US5422239A (en) * | 1980-09-19 | 1995-06-06 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
| US5011771A (en) * | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
| FR2710410A1 (fr) * | 1993-09-20 | 1995-03-31 | Bio Merieux | Procédé et dispositif pour la détermination d'un analyte dans un échantillon . |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2074727B (en) | 1983-11-30 |
| FR2481318B1 (enrdf_load_stackoverflow) | 1984-10-19 |
| NO811407L (no) | 1981-10-26 |
| CA1160566A (en) | 1984-01-17 |
| IT1137344B (it) | 1986-09-10 |
| IT8121122A0 (it) | 1981-04-13 |
| AU542563B2 (en) | 1985-02-28 |
| NL187545B (nl) | 1991-06-03 |
| DK155881A (da) | 1981-10-26 |
| DK151399B (da) | 1987-11-30 |
| NL8101860A (nl) | 1981-11-16 |
| DE3115115A1 (de) | 1982-02-04 |
| AT373398B (de) | 1984-01-10 |
| ATA186481A (de) | 1983-05-15 |
| AU6981181A (en) | 1981-10-29 |
| SE8102558L (sv) | 1981-10-26 |
| GB2074727A (en) | 1981-11-04 |
| DK151399C (da) | 1988-09-05 |
| NO159620C (no) | 1989-01-18 |
| SE460930B (sv) | 1989-12-04 |
| NO159620B (no) | 1988-10-10 |
| DE3115115C2 (de) | 1987-02-12 |
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