FI68324C - Specifik bindningsanlysmetod och reagensmedel foer anvaendningdaervid - Google Patents

Specifik bindningsanlysmetod och reagensmedel foer anvaendningdaervid Download PDF

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Publication number
FI68324C
FI68324C FI761148A FI761148A FI68324C FI 68324 C FI68324 C FI 68324C FI 761148 A FI761148 A FI 761148A FI 761148 A FI761148 A FI 761148A FI 68324 C FI68324 C FI 68324C
Authority
FI
Finland
Prior art keywords
specific binding
ligand
coenzyme
reagent
bound
Prior art date
Application number
FI761148A
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English (en)
Finnish (fi)
Other versions
FI68324B (fi
FI761148A (sv
Inventor
Robert Charles Boguslaski
Robert Joseph Carrico
James Edward Christner
Original Assignee
Miles Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Lab filed Critical Miles Lab
Publication of FI761148A publication Critical patent/FI761148A/fi
Priority to FI840616A priority Critical patent/FI69214C/fi
Priority to FI840617A priority patent/FI68915C/fi
Publication of FI68324B publication Critical patent/FI68324B/fi
Application granted granted Critical
Publication of FI68324C publication Critical patent/FI68324C/fi

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/44—Iso-indoles; Hydrogenated iso-indoles
    • C07D209/48—Iso-indoles; Hydrogenated iso-indoles with oxygen atoms in positions 1 and 3, e.g. phthalimide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D237/00—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings
    • C07D237/26—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings condensed with carbocyclic rings or ring systems
    • C07D237/30—Phthalazines
    • C07D237/32—Phthalazines with oxygen atoms directly attached to carbon atoms of the nitrogen-containing ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Claims (11)

1. Specifik bindningsanalysmetod för bestämning av en ligand i ett flytande medium, vilken metod omfattar följande steg: (a) man bringar det flytande mediet i beröring med ett reagens-medel, som innehäller ett konjugat av ett märkningsmedel och ett specifikt bindande medel, varvid reagensmedlet och liganden bildar ett bindningsreaktionssystem, som bildar (i) en bunden fas av det märkta konjugatet, väri det specifikt bindande mediet är bundet därvid med ett specifikt bindningspar och (ii) en fri fas av det märkta konjugatet, väri det specifikt bindande mediet ej är bundet därvid med ett specifikt bindningspar, och (b) man bestämmer märkningsmedlet antingen i den bundna eller den fria fasen som ett mätt för liganden i det flytande mediet, kännetecknad av att märkningsmedlet är ett koenzym och att metoden är av homogen typ, varvid den bundna fasen och den fria fasen av det koenzym-märkta konjugatet ej skiljs fysi-kaliskt frän varandra och varvid koenzymets aktivitet i den flytande bulkreaktionsblandningen mäts och ställs i relation till mängden ligand närvarande i det testade mediet.
2. Metod enligt patentkravet 1,kännetecknad av att koenzymet är ett nukleotidiskt Icoenzym.
3. Metod enligt patentkravet 1,kännetecknad av att koenzymet är ett adenosinfosfat, nikotinamid-adenin-dinuk-leotid eller en reducerad form därav, eller nikotinamid-adenin-dinukleotidfosfat eller en reducerad form därav. h. Metod enligt patentkravet 1,kännetecknad av att koenzymet är adenosintrifosfat.
5. Metod enligt ptatentkravet 1, kännet ecknad av att koenzymet är flavinadenin-dinukleotid.
6. Reagensmedel för bestämning av en ligand i ett flytande medium, vilket reagensmedel omfattar ett konjugat av ett märk-
FI761148A 1975-04-28 1976-04-26 Specifik bindningsanlysmetod och reagensmedel foer anvaendningdaervid FI68324C (fi)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FI840616A FI69214C (fi) 1975-04-28 1984-02-15 Specifik bindningsanalysmetod och reagens foer anvaendning daervid
FI840617A FI68915C (fi) 1975-04-28 1984-02-15 Specifik bindningsanalysmetod och reagens foer anvaendning daervid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57200875A 1975-04-28 1975-04-28
US57200875 1975-04-28

Publications (3)

Publication Number Publication Date
FI761148A FI761148A (sv) 1976-10-29
FI68324B FI68324B (fi) 1985-04-30
FI68324C true FI68324C (fi) 1985-08-12

Family

ID=24285954

Family Applications (1)

Application Number Title Priority Date Filing Date
FI761148A FI68324C (fi) 1975-04-28 1976-04-26 Specifik bindningsanlysmetod och reagensmedel foer anvaendningdaervid

Country Status (23)

Country Link
JP (6) JPS6052378B2 (sv)
AT (1) AT357685B (sv)
AU (2) AU509182B2 (sv)
BE (1) BE841179A (sv)
BR (1) BR7602561A (sv)
CA (2) CA1078712A (sv)
CH (1) CH635438A5 (sv)
DD (1) DD125231A5 (sv)
DE (3) DE2618419C2 (sv)
DK (3) DK149969C (sv)
ES (1) ES447378A1 (sv)
FI (1) FI68324C (sv)
FR (1) FR2332533A1 (sv)
GB (2) GB1552607A (sv)
HU (1) HU179542B (sv)
IE (1) IE42544B1 (sv)
IL (1) IL49354A (sv)
IN (1) IN142734B (sv)
IT (1) IT1064132B (sv)
LU (1) LU74834A1 (sv)
NL (1) NL181281C (sv)
SE (3) SE440280B (sv)
ZA (1) ZA762030B (sv)

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DE2924249C2 (de) * 1978-06-22 1989-04-27 Miles, Inc., Elkhart, Ind., Us Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode
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JPS5877843U (ja) * 1981-11-20 1983-05-26 株式会社三協精機製作所 テ−プレコ−ダ
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Also Published As

Publication number Publication date
IT1064132B (it) 1985-02-18
DK150808C (da) 1988-02-15
SE451895B (sv) 1987-11-02
GB1552607A (en) 1979-09-19
DK576281A (da) 1981-12-23
JPS5951353A (ja) 1984-03-24
SE8007397L (sv) 1980-10-21
HU179542B (en) 1982-11-29
IE42544B1 (en) 1980-08-27
FI68324B (fi) 1985-04-30
SE8007396L (sv) 1980-10-21
IL49354A0 (en) 1976-06-30
ES447378A1 (es) 1977-11-01
ZA762030B (en) 1977-08-31
CA1078712A (en) 1980-06-03
JPS51146295A (en) 1976-12-15
DK158366C (da) 1990-10-01
DE2618419C2 (de) 1983-02-10
IN142734B (sv) 1977-08-20
AU502726B2 (en) 1979-08-09
BR7602561A (pt) 1976-11-23
CH635438A5 (en) 1983-03-31
JPS6052378B2 (ja) 1985-11-19
DE2660548C2 (de) 1984-04-12
DK576181A (da) 1981-12-23
FR2332533B1 (sv) 1981-12-18
FI761148A (sv) 1976-10-29
IL49354A (en) 1979-03-12
FR2332533A1 (fr) 1977-06-17
JPS604939B2 (ja) 1985-02-07
DD125231A5 (sv) 1977-04-06
SE440280B (sv) 1985-07-22
SE7604841L (sv) 1976-10-29
NL181281C (nl) 1987-07-16
AT357685B (de) 1980-07-25
DK189176A (da) 1976-10-29
AU509182B2 (en) 1980-04-24
JPS5951354A (ja) 1984-03-24
SE447510B (sv) 1986-11-17
DE2618511C3 (de) 1981-05-07
DE2618511A1 (de) 1976-11-04
DK149969B (da) 1986-11-03
JPS5267388A (en) 1977-06-03
DK150808B (da) 1987-06-22
DK158366B (da) 1990-05-07
GB1548741A (en) 1979-07-18
NL7604420A (nl) 1976-11-01
DE2618511B2 (de) 1980-08-14
AU1335376A (en) 1977-11-03
AU1335276A (en) 1977-11-03
JPH0145027B2 (sv) 1989-10-02
DE2618419A1 (de) 1976-11-04
IE42544L (en) 1976-10-28
NL181281B (nl) 1987-02-16
JPS5942454A (ja) 1984-03-09
JPH0137693B2 (sv) 1989-08-09
JPH0137694B2 (sv) 1989-08-09
CA1082577A (en) 1980-07-29
JPS5942453A (ja) 1984-03-09
BE841179A (fr) 1976-08-16
LU74834A1 (sv) 1977-01-12
DK149969C (da) 1988-08-01
JPH0145026B2 (sv) 1989-10-02
ATA309376A (de) 1979-12-15

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