FI63596C - Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser - Google Patents

Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser Download PDF

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Publication number
FI63596C
FI63596C FI813251A FI813251A FI63596C FI 63596 C FI63596 C FI 63596C FI 813251 A FI813251 A FI 813251A FI 813251 A FI813251 A FI 813251A FI 63596 C FI63596 C FI 63596C
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Prior art keywords
nucleic acid
reagent
fragment
labeled
plasmid
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FI813251A
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English (en)
Finnish (fi)
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FI63596B (fi
Inventor
Tuula Marjut Ranki
Hans Erik Soederlund
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Orion Yhtymae Oy
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Publication date
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Priority to FI813251A priority Critical patent/FI63596C/fi
Priority to PCT/FI1982/000038 priority patent/WO1983001459A1/en
Priority to JP57502956A priority patent/JPS58501703A/ja
Priority to HU823529A priority patent/HU196242B/hu
Priority to AU89575/82A priority patent/AU548854B2/en
Priority to EP82902982A priority patent/EP0098267A1/en
Priority to JP57502956A priority patent/JPH0632637B1/ja
Priority to FI823452A priority patent/FI823452A0/fi
Priority to US06/434,182 priority patent/US4486539A/en
Priority to DE8282305489T priority patent/DE3277917D1/de
Priority to AT82305489T priority patent/ATE31735T1/de
Priority to EP82305489A priority patent/EP0079139B1/en
Priority to CA000413539A priority patent/CA1192120A/en
Priority to DE198282305489T priority patent/DE79139T1/de
Publication of FI63596B publication Critical patent/FI63596B/fi
Priority to NO83832061A priority patent/NO163699C/no
Priority to DK198302751A priority patent/DK173744B1/da
Application granted granted Critical
Publication of FI63596C publication Critical patent/FI63596C/fi
Priority to RO112563A priority patent/RO86356B/ro
Priority to SU833663405A priority patent/SU1386031A3/ru
Priority to US06/566,532 priority patent/US4563419A/en

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    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/823—Immunogenic carrier or carrier per se

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
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  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
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  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
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  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)

Claims (10)

1. Mikrobdiagnostiskt förfarande för samtidig identifiering av en eller flera mikrober och/eller mikrobgrupper, vilket 5 förfarande grundar sig pi skiktshybridisering av nuklein-syror pa enfast bärare, i vilket förfarande används tvi olika nukleinsyrareagenser, av vilka det ena bestar av ett enkelstrangat nukleinsyrafragment, bundet vid den fasta bäraren, och det andra bestar av ett enkelstrangat nuklein-10 syrafragment märkt med ett lämpligt ämne, kanne-tecknat därav, att en serie, bestaende av minst tva nukleinsyrareagenser för varje mikrob och/eller mikrob-grupp, som är avsedd att pivisas, av vilka reagenser det ena är bundet vid den fasta bäraren och det andra är 15 märkt med ett i och för sig känt ämne, försätts i kontakt med ett enda odelat prov, vilket innehiller nuklein-syror frin de mikrober och/eller mikrobgrupper, vilka är avscuida att pivisas och vilka nukleinsyror gjorts enkelsträngade med i och för sig kända förfaranden, 20 varvid av nukleinsyrorna i nukleinsyrablandningen frin provet endast de, mot vilka svarande nukleinsyrareagenser används hybridiseras med de motsvarande, vid den fasta bäraren bundna, identifierande nukleinsyrafragmenten och de därvid bildade vid den fasta bäraren bundna 25 hybriderna blir märkta med hjälp av de motsvarande, märkta nukleinsyrorna, di dessa binder sig vid de frin provet härstammande vid den fasta bäraren fästa nukleinsyrorna och den fasta bärarens märkning mäts med i och för sig kända förfaranden.
2. En serie reagenskombinationer avsedd att användas i ett förfarande enligt patentkrav 1, kanne- t e c k n a d därav, att tili serien för varje mikrob och/eller mikrobgrupp avsedd att identifieras hör 35 minst tvi nukleinsyrareagenser, för vilka reagensers framställning.behövliga tvi frin mikrober och/ eller mikroljgrupper härstammande olika nukleinsyrafragment har framställts frin mikrobgenomets olika delar men förminligt närä varandra belägna fragment, 63596 30 antingen direkt frln mikrobgenomet eller genom att tillgodogöra sig i och för sig känd rekombinations-DNA-teknik och dessa, för varje mikrob specifika, antingen grupp- eller artspecifika nukleinsyrafragment, har gjorts 5 enkel s trängade och det ena fragmentet har fästs vid en fast bärare och det andra har märkts med ett ämne.
3. En serie reagenskombinationer enligt patentkrav 2, kännetecknad därav, att den för identifiering 10 av adenovirus omfattar som nukleinsyrareagens bundet vid den fasta bararen adenovirus rekombinantplasmid Ad2ÖpBR322 och som märkt nukleinsyrareagens, adenovirus Ad2BamHI C-fragment.
4. En serie reagenskombinationer enligt patentkrav 2, kännetecknad därav, att den för identifiering av Semliki Forest-virus omfattar som nukleinsyrareagens bundet vid den fasta bararen pKTH312^plasmidens EcoRI-Xhol fragment A och som märkt nukleinsyrareagens pKTH312-20 plasmidens EcoRI-XhoI fragment B.
5. En serie reagenskombinationer enligt patentkrav 2, kännetecknad därav, att den för identifiering av SV40-virus omfattar som nukleinsyrareagens bundet 25 vid den fasta bäraren SV40-virus PstI B-fragment och som märkt nukleinsyrareagens SV40-virus PstI A-fragment. 1 II En serie reagenskombinationer enligt patentkrav 2, kännetecknad därav, att den för identifiering 30 av Bacillus amyloliquefaciens omfattar som nukleinsyrareagens bundet vid den fasta bäraren CX -amylasgenens EcoRI-BamHI-fragment i Bacillus amyloliquefaciens pKTHIO plasmid och som märkt nukleinsyrareagens o(-amy-lasgenens Clal-EcoRI-fragment i Bacillus amyloliquefaciens 35 pKTHIO plasmid.
FI813251A 1981-10-16 1981-10-16 Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser FI63596C (fi)

Priority Applications (19)

Application Number Priority Date Filing Date Title
FI813251A FI63596C (fi) 1981-10-16 1981-10-16 Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
PCT/FI1982/000038 WO1983001459A1 (en) 1981-10-16 1982-09-29 A method and reagent combination for the diagnosis of microorganisms by sandwich hybridization of nucleic acids
JP57502956A JPS58501703A (ja) 1981-10-16 1982-09-29 微生物鑑別方法及び微生物鑑別試剤の組み合わせ
HU823529A HU196242B (en) 1981-10-16 1982-09-29 Process and reagent combination for identifying microorganisms by sandwich-hybridization of nucleic acids
AU89575/82A AU548854B2 (en) 1981-10-16 1982-09-29 A method and reagent combination for the diagnosis of microorganisms
EP82902982A EP0098267A1 (en) 1981-10-16 1982-09-29 A method and reagent combination for the diagnosis of microorganisms by sandwich hybridization of nucleic acids
JP57502956A JPH0632637B1 (sv) 1981-10-16 1982-09-29
FI823452A FI823452A0 (fi) 1981-10-16 1982-10-11 Foerfarande foer stabilisering av i nukleinsyrehybridisering anvaenda filter
US06/434,182 US4486539A (en) 1981-10-16 1982-10-14 Detection of microbial nucleic acids by a one-step sandwich hybridization test
DE8282305489T DE3277917D1 (en) 1981-10-16 1982-10-15 A method and reagent combination for the identification of microorganisms and the use of sandwich hybridization of nucleic acids therefor
AT82305489T ATE31735T1 (de) 1981-10-16 1982-10-15 Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer.
EP82305489A EP0079139B1 (en) 1981-10-16 1982-10-15 A method and reagent combination for the identification of microorganisms and the use of sandwich hybridization of nucleic acids therefor
CA000413539A CA1192120A (en) 1981-10-16 1982-10-15 Method and reagent combination for the diagnosis of microorganisms
DE198282305489T DE79139T1 (de) 1981-10-16 1982-10-15 Methode und reagenskombination zur indentifikation von mikroorganismen und die anwendung der sandwichhybridisierung von nukleinsaeuren hierfuer.
NO83832061A NO163699C (no) 1981-10-16 1983-06-07 Fremgangsmaate og reagenskombinasjon for diagnose av mikroorganismer.
DK198302751A DK173744B1 (da) 1981-10-16 1983-06-15 Fremgangsmåde og reagenskombination til diagnose af mikroorganismer
RO112563A RO86356B (ro) 1981-10-16 1983-11-15 Metoda de identificare microbiana
SU833663405A SU1386031A3 (ru) 1981-10-16 1983-11-15 Способ идентификации вирусов и бактерий
US06/566,532 US4563419A (en) 1981-10-16 1983-12-29 Detection of microbial nucleic acids by a one-step sandwich hybridization test

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI813251A FI63596C (fi) 1981-10-16 1981-10-16 Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
FI813251 1981-10-16

Publications (2)

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FI63596B FI63596B (fi) 1983-03-31
FI63596C true FI63596C (fi) 1983-07-11

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FI813251A FI63596C (fi) 1981-10-16 1981-10-16 Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser

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US (2) US4486539A (sv)
EP (2) EP0098267A1 (sv)
JP (2) JPS58501703A (sv)
AT (1) ATE31735T1 (sv)
AU (1) AU548854B2 (sv)
CA (1) CA1192120A (sv)
DE (2) DE3277917D1 (sv)
DK (1) DK173744B1 (sv)
FI (1) FI63596C (sv)
HU (1) HU196242B (sv)
RO (1) RO86356B (sv)
SU (1) SU1386031A3 (sv)
WO (1) WO1983001459A1 (sv)

Families Citing this family (341)

* Cited by examiner, † Cited by third party
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RO86356B (ro) 1985-04-01
HU196242B (en) 1988-10-28
DK173744B1 (da) 2001-09-03
WO1983001459A1 (en) 1983-04-28
AU548854B2 (en) 1986-01-02
SU1386031A3 (ru) 1988-03-30
CA1192120A (en) 1985-08-20
ATE31735T1 (de) 1988-01-15
AU8957582A (en) 1983-05-05
DE3277917D1 (en) 1988-02-11
US4563419A (en) 1986-01-07
JPH0632637B1 (sv) 1994-05-02
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RO86356A (ro) 1985-03-15
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