GR1000347B - Μεθοδος παραγωγης νουκλεοτιδικων ανιχνευτων χρησιμοποιωντας ενα συμπληρωμα γεφυρωσεως. - Google Patents
Μεθοδος παραγωγης νουκλεοτιδικων ανιχνευτων χρησιμοποιωντας ενα συμπληρωμα γεφυρωσεως.Info
- Publication number
- GR1000347B GR1000347B GR890100835A GR890100835A GR1000347B GR 1000347 B GR1000347 B GR 1000347B GR 890100835 A GR890100835 A GR 890100835A GR 890100835 A GR890100835 A GR 890100835A GR 1000347 B GR1000347 B GR 1000347B
- Authority
- GR
- Greece
- Prior art keywords
- nucleotide probes
- preparing nucleotide
- bridging
- complement
- bridging complement
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000002773 nucleotide Substances 0.000 title abstract 2
- 125000003729 nucleotide group Chemical group 0.000 title abstract 2
- 230000000295 complement effect Effects 0.000 title 1
- 239000000523 sample Substances 0.000 title 1
- 239000012634 fragment Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Abstract
Η παρούσα εφεύρεση δίδει μία εναλλακτική λύση δια την σύνθεση τεμαχίων νουκλεϊκού οξέος γνωστών εις την τεχνική. Η μέθοδος παράγει ένα ιχνοθετημένο τεμάχιο νουκλεοτιδίου το οποίο έχει ένα πρώτο "υπόλοιπον αναγνωρίσεως στόχου" και ένα δεύτερο "υπόλοιπον παραγωγής σήματος". 'Εκαστον υπόλοιπον περιέχει ένα άκρον 3' και 5' όπου τα δύο υπόλοιπα είναι συνδεδεμένα το ένα με το άλλο εις στερεοδιάταξιν 3'-5'. ω
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28743188A | 1988-12-21 | 1988-12-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
GR890100835A GR890100835A (en) | 1991-03-15 |
GR1000347B true GR1000347B (el) | 1992-06-25 |
Family
ID=23102876
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GR890100835A GR1000347B (el) | 1988-12-21 | 1989-12-19 | Μεθοδος παραγωγης νουκλεοτιδικων ανιχνευτων χρησιμοποιωντας ενα συμπληρωμα γεφυρωσεως. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5538872A (el) |
EP (1) | EP0387452B1 (el) |
JP (1) | JPH02238900A (el) |
AT (1) | ATE125546T1 (el) |
CA (1) | CA2005927A1 (el) |
DE (1) | DE68923608T2 (el) |
ES (1) | ES2076220T3 (el) |
GR (1) | GR1000347B (el) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
US6022689A (en) * | 1995-04-07 | 2000-02-08 | University Of New Mexico | Situ hybridization slide processes |
AU1428097A (en) * | 1996-01-12 | 1997-08-01 | Hybridon, Inc. | Method of monitoring pharmacokinetics of oligonucleotide pharmaceuticals |
US20040266706A1 (en) * | 2002-11-05 | 2004-12-30 | Muthiah Manoharan | Cross-linked oligomeric compounds and their use in gene modulation |
US6218160B1 (en) | 1997-10-31 | 2001-04-17 | Roche Diagnostics Corporation | Site-specific conjugation of glycoproteins |
WO2000046232A1 (en) * | 1999-02-05 | 2000-08-10 | Integrated Dna Technologies, Inc. | Primer extension methods for production of high specific activity nucleic acid probes |
US7135284B1 (en) | 1999-02-05 | 2006-11-14 | Integrated Dna Technologies, Inc. | Primer extension methods for production of high specific activity nucleic acid probes |
AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
US20030194734A1 (en) * | 2002-03-29 | 2003-10-16 | Tim Jatkoe | Selection of markers |
GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
CN100357736C (zh) * | 2003-07-25 | 2007-12-26 | 大连康基生物技术有限公司 | 沙门氏菌快速检测装置 |
US8232049B2 (en) * | 2006-09-29 | 2012-07-31 | Case Western Reserve University | Method for detecting small oligonucleotides |
US8685899B2 (en) | 2007-02-14 | 2014-04-01 | Genisphere Inc. | Methods, reagents and kits for detection of nucleic acid molecules |
US20100190167A1 (en) * | 2007-02-14 | 2010-07-29 | Genisphere Inc. | Methods, Reagents and Kits for Detection of Nucleic Acid Molecules |
CN102135537A (zh) * | 2010-09-03 | 2011-07-27 | 李克生 | 志贺氏菌检测方法及其金标快速诊断试剂盒和制备方法 |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4711955A (en) * | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
EP0135587B2 (en) * | 1983-02-22 | 2002-12-18 | Syngene, Inc. | Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis |
GB8311018D0 (en) * | 1983-04-22 | 1983-05-25 | Amersham Int Plc | Detecting mutations in dna |
US4699876A (en) * | 1984-01-27 | 1987-10-13 | E. I. Du Pont De Nemours And Company | Nonradiometric polynucleotide probes |
US4707440A (en) * | 1984-01-30 | 1987-11-17 | Enzo Biochem, Inc. | Nucleic acid hybridization assay and detectable molecules useful in such assay |
WO1985004674A1 (en) * | 1984-04-05 | 1985-10-24 | Life Technologies, Inc. | Immobilization of nucleic acids |
US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
CA1278755C (en) * | 1985-03-15 | 1991-01-08 | William J. Knowles | Labelling of oligonucleotides |
US4797355A (en) * | 1985-06-13 | 1989-01-10 | Amgen Inc. | Methods for attaching polynucleotides to supports |
CA1295559C (en) * | 1985-08-13 | 1992-02-11 | Jannis Stavrianopoulos | Method for labeling polynucleotide sequences |
US4806631A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on solvolyzed nylon supports |
EP0224126A3 (en) * | 1985-11-25 | 1989-02-01 | The University of Calgary | Covalently linked complementary oligodeoxynucleotides as universal nucleic acid sequencing primer linkers |
JP3293820B2 (ja) * | 1985-12-13 | 2002-06-17 | エンゾ− バイオケム インコ−ポレイテツド | 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物 |
US4900659A (en) * | 1986-01-30 | 1990-02-13 | Enzo Biochem, Inc. | Nucleotide sequence composition and method for detection of Neisseria gonorrhoeae and method for screening for a nucleotide sequence that is specific for a genetically distinct group |
NO870613L (no) * | 1986-03-05 | 1987-09-07 | Molecular Diagnostics Inc | Deteksjon av mikroorganismer i en prve inneholdende nukleinsyre. |
GB8612087D0 (en) * | 1986-05-19 | 1986-06-25 | Ici Plc | Hybridisation probes |
EP0276302B1 (en) * | 1986-08-11 | 1993-04-28 | Siska Diagnostics,Inc. | Nucleic acid probe assay methods and compositions |
US4794082A (en) * | 1987-02-17 | 1988-12-27 | Hoechst Celanese Corporation | Biotinylating agents |
IL86164A0 (en) * | 1987-04-28 | 1988-11-15 | Tamir Biotechnology Ltd | Improved dna probes |
CA1323293C (en) * | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
DE3888653T2 (de) * | 1987-12-21 | 1994-07-07 | Applied Biosystems | Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz. |
US5449602A (en) * | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
US5922754A (en) * | 1998-10-02 | 1999-07-13 | Abbott Laboratories | Pharmaceutical compositions containing paclitaxel |
-
1989
- 1989-12-19 GR GR890100835A patent/GR1000347B/el unknown
- 1989-12-19 CA CA002005927A patent/CA2005927A1/en not_active Abandoned
- 1989-12-20 EP EP89313376A patent/EP0387452B1/en not_active Expired - Lifetime
- 1989-12-20 JP JP1328626A patent/JPH02238900A/ja active Pending
- 1989-12-20 ES ES89313376T patent/ES2076220T3/es not_active Expired - Lifetime
- 1989-12-20 DE DE68923608T patent/DE68923608T2/de not_active Expired - Lifetime
- 1989-12-20 AT AT89313376T patent/ATE125546T1/de not_active IP Right Cessation
-
1994
- 1994-09-08 US US08/302,332 patent/US5538872A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
GR890100835A (en) | 1991-03-15 |
CA2005927A1 (en) | 1990-06-21 |
JPH02238900A (ja) | 1990-09-21 |
DE68923608D1 (de) | 1995-08-31 |
EP0387452A3 (en) | 1991-04-10 |
EP0387452B1 (en) | 1995-07-26 |
ATE125546T1 (de) | 1995-08-15 |
ES2076220T3 (es) | 1995-11-01 |
US5538872A (en) | 1996-07-23 |
DE68923608T2 (de) | 1996-01-04 |
EP0387452A2 (en) | 1990-09-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GR1000347B (el) | Μεθοδος παραγωγης νουκλεοτιδικων ανιχνευτων χρησιμοποιωντας ενα συμπληρωμα γεφυρωσεως. | |
FI911152A0 (fi) | Amplifikationsfoerfaranden av nukleinsyrasekvens. | |
NO851689L (no) | Fremgangsmaate ved paavisning av et genetisk materiale | |
EP0185444A3 (en) | Cloning and expression of htlv-iii dna | |
DE3889091T2 (de) | Härtbare Zusammensetzung von Oxyalkylenpolymeren. | |
DE3585953D1 (de) | Dna-fragmente, expressionsvektoren, proteine, wirte und verfahren zur herstellung der proteine. | |
DK110885A (da) | Nucleinsyre-proteinkonjugater, disses anvendelse samt fremgangsmaade til fremstilling heraf | |
FR2637612B1 (fr) | Sequences de nucleotides codant pour une proteine a activite ureasique | |
DE3586090D1 (de) | Nachweis der spezifischen sequenzen in nukleinsaeuren. | |
NL300197I1 (el) | ||
NO883670L (no) | Metode for direktebestemmelse av fysikalske egenskaper for hydrokarbonprodukter. | |
GR1000315B (el) | Βελτιωμενη μεθοδος ποσοτικου προσδιορισμου νουκλεινικων οξεων,συνδυασμος αντιδραστηρiων και ενα "κιτ" αυτων. | |
ATE236269T1 (de) | Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation | |
DE3575550D1 (de) | Verfahren zur umsetzung von alkanen mit niedrigem molekulargewicht. | |
DE69034197D1 (de) | Genomische Klonierung und Kartographie | |
DE3686376T2 (de) | Signalverarbeitungsverfahren zur bestimmung der basissequenz von nukleinsaeure. | |
NO885013D0 (no) | Fremgangsmte for fremstilling av bionedbrytbare mikrokapsler av vannopplelige peptider og proteiner, samt mikroka psler oppndd ved fremgangsmten. | |
ATE175237T1 (de) | Polypeptide beteiligt an der kobalamin- und/oder kobamid-biosynthese, für diese kodierende dns- sequencen, und ihre herstellung und vewendung. | |
GR1000715B (el) | Μεθοδος για την παρασκευη πολυ-νουκλεοτιδιων. | |
DE69535024D1 (de) | Verpackungszellinie zur transkomplementierung von defektiven retroviren-vektoren | |
FI864626A0 (fi) | Rekombinantricintoxin. | |
BR8807891A (pt) | Processo de fiacao em fusao de polimero de poliester com alto peso molecular | |
SU580440A1 (ru) | Датчик дл измерени параметров трещины | |
DD138194A1 (de) | Verfahren zur herstellung von molekularsiebgranulaten mit verbesserten mechanischen festigkeiten | |
ES539964A0 (es) | Metodo de detectar acidos nucleicos. |