DE68923608D1 - Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements. - Google Patents

Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements.

Info

Publication number
DE68923608D1
DE68923608D1 DE68923608T DE68923608T DE68923608D1 DE 68923608 D1 DE68923608 D1 DE 68923608D1 DE 68923608 T DE68923608 T DE 68923608T DE 68923608 T DE68923608 T DE 68923608T DE 68923608 D1 DE68923608 D1 DE 68923608D1
Authority
DE
Germany
Prior art keywords
nucleotide probes
complementary element
preparing nucleotide
hybridizable
hybridizable complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68923608T
Other languages
English (en)
Other versions
DE68923608T2 (de
Inventor
Chandler Dr Bahl
Leopoldo Mendoza
Frank J Mcmahon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Ortho Diagnostic Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Diagnostic Systems Inc filed Critical Ortho Diagnostic Systems Inc
Publication of DE68923608D1 publication Critical patent/DE68923608D1/de
Application granted granted Critical
Publication of DE68923608T2 publication Critical patent/DE68923608T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE68923608T 1988-12-21 1989-12-20 Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements. Expired - Lifetime DE68923608T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28743188A 1988-12-21 1988-12-21

Publications (2)

Publication Number Publication Date
DE68923608D1 true DE68923608D1 (de) 1995-08-31
DE68923608T2 DE68923608T2 (de) 1996-01-04

Family

ID=23102876

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68923608T Expired - Lifetime DE68923608T2 (de) 1988-12-21 1989-12-20 Verfahren zur Vorbereitung von Nukleotid-Sonden unter Verwendung eines hybridizierbaren Komplementärelements.

Country Status (8)

Country Link
US (1) US5538872A (de)
EP (1) EP0387452B1 (de)
JP (1) JPH02238900A (de)
AT (1) ATE125546T1 (de)
CA (1) CA2005927A1 (de)
DE (1) DE68923608T2 (de)
ES (1) ES2076220T3 (de)
GR (1) GR1000347B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6022689A (en) * 1995-04-07 2000-02-08 University Of New Mexico Situ hybridization slide processes
AU1428097A (en) * 1996-01-12 1997-08-01 Hybridon, Inc. Method of monitoring pharmacokinetics of oligonucleotide pharmaceuticals
US20040266706A1 (en) * 2002-11-05 2004-12-30 Muthiah Manoharan Cross-linked oligomeric compounds and their use in gene modulation
US6218160B1 (en) 1997-10-31 2001-04-17 Roche Diagnostics Corporation Site-specific conjugation of glycoproteins
US7135284B1 (en) 1999-02-05 2006-11-14 Integrated Dna Technologies, Inc. Primer extension methods for production of high specific activity nucleic acid probes
WO2000046232A1 (en) * 1999-02-05 2000-08-10 Integrated Dna Technologies, Inc. Primer extension methods for production of high specific activity nucleic acid probes
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US20030194734A1 (en) * 2002-03-29 2003-10-16 Tim Jatkoe Selection of markers
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
CN100357736C (zh) * 2003-07-25 2007-12-26 大连康基生物技术有限公司 沙门氏菌快速检测装置
US8232049B2 (en) * 2006-09-29 2012-07-31 Case Western Reserve University Method for detecting small oligonucleotides
US20100190167A1 (en) * 2007-02-14 2010-07-29 Genisphere Inc. Methods, Reagents and Kits for Detection of Nucleic Acid Molecules
US8685899B2 (en) * 2007-02-14 2014-04-01 Genisphere Inc. Methods, reagents and kits for detection of nucleic acid molecules
CN102135537A (zh) * 2010-09-03 2011-07-27 李克生 志贺氏菌检测方法及其金标快速诊断试剂盒和制备方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
EP0135587B2 (de) * 1983-02-22 2002-12-18 Syngene, Inc. Einzelstrang-oligonukleotide bestimmter sequenz, markergruppen enthaltend, verfahren zu deren chemischer synthese und nukleotide zur verwendung darin
GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
US4699876A (en) * 1984-01-27 1987-10-13 E. I. Du Pont De Nemours And Company Nonradiometric polynucleotide probes
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
JPS61501746A (ja) * 1984-04-05 1986-08-21 ライフ　テクノロジ−ズ，インコ−ポレイテツド 核酸の固定化法
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
CA1278755C (en) * 1985-03-15 1991-01-08 William J. Knowles Labelling of oligonucleotides
US4797355A (en) * 1985-06-13 1989-01-10 Amgen Inc. Methods for attaching polynucleotides to supports
CA1295559C (en) * 1985-08-13 1992-02-11 Jannis Stavrianopoulos Method for labeling polynucleotide sequences
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
EP0224126A3 (de) * 1985-11-25 1989-02-01 The University of Calgary Kovalent gekoppelte komplementäre Oligodeoxynukleotide zur Verwendung als "Primer Linkers" bei Sequenzierung von Nukleinsäuren
JP3293820B2 (ja) * 1985-12-13 2002-06-17 エンゾ− バイオケム インコ−ポレイテツド 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物
US4900659A (en) * 1986-01-30 1990-02-13 Enzo Biochem, Inc. Nucleotide sequence composition and method for detection of Neisseria gonorrhoeae and method for screening for a nucleotide sequence that is specific for a genetically distinct group
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
GB8612087D0 (en) * 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
CA1308372C (en) * 1986-08-11 1992-10-06 Geneva Ruth Davis Nucleic acid probe sandwich assay methods and compositions
US4794082A (en) * 1987-02-17 1988-12-27 Hoechst Celanese Corporation Biotinylating agents
IL86164A0 (en) * 1987-04-28 1988-11-15 Tamir Biotechnology Ltd Improved dna probes
AU622426B2 (en) * 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
EP0322311B1 (de) * 1987-12-21 1994-03-23 Applied Biosystems, Inc. Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz
US5449602A (en) * 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5922754A (en) * 1998-10-02 1999-07-13 Abbott Laboratories Pharmaceutical compositions containing paclitaxel

Also Published As

Publication number Publication date
CA2005927A1 (en) 1990-06-21
JPH02238900A (ja) 1990-09-21
GR890100835A (en) 1991-03-15
US5538872A (en) 1996-07-23
EP0387452A2 (de) 1990-09-19
DE68923608T2 (de) 1996-01-04
EP0387452A3 (de) 1991-04-10
EP0387452B1 (de) 1995-07-26
ATE125546T1 (de) 1995-08-15
ES2076220T3 (es) 1995-11-01
GR1000347B (el) 1992-06-25

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8328 Change in the person/name/address of the agent

Representative=s name: BOEHMERT & BOEHMERT, 28209 BREMEN