ES2374910T3 - Polimerasas de adn termoestables modificadas para secuenciación. - Google Patents
Polimerasas de adn termoestables modificadas para secuenciación. Download PDFInfo
- Publication number
- ES2374910T3 ES2374910T3 ES98116786T ES98116786T ES2374910T3 ES 2374910 T3 ES2374910 T3 ES 2374910T3 ES 98116786 T ES98116786 T ES 98116786T ES 98116786 T ES98116786 T ES 98116786T ES 2374910 T3 ES2374910 T3 ES 2374910T3
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- Prior art keywords
- dna
- amino acid
- polymerase
- labeled
- xaa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
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- Zoology (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
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- Biochemistry (AREA)
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- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
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| US5852597P | 1997-09-11 | 1997-09-11 | |
| US58525P | 1997-09-11 |
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| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) * | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US20030138834A1 (en) * | 1998-08-19 | 2003-07-24 | Dawson Elliott P. | Method for determining polynucleotide sequence variations |
| US20040121394A1 (en) * | 1998-08-19 | 2004-06-24 | Dawson Elliott P. | Method for determining polynucleotide sequence variations |
| US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7501245B2 (en) * | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
| WO2001014568A1 (en) * | 1999-08-21 | 2001-03-01 | Amersham Pharmacia Biotech Inc. | Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance |
| US7179590B2 (en) * | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| US6214557B1 (en) * | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| WO2002072892A1 (en) | 2001-03-12 | 2002-09-19 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
| ATE549415T1 (de) * | 2001-03-16 | 2012-03-15 | Kalim Mir | Arrays und verfahren zur deren verwendung |
| US6875573B2 (en) | 2001-07-06 | 2005-04-05 | Amersham Biosciences Corp | DNA polymerases having amino acid substitutions and homologs thereof |
| US20030228589A1 (en) * | 2002-02-12 | 2003-12-11 | Bond-Nutter Diane R. | Polymerase compositions |
| DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
| KR100571808B1 (ko) * | 2003-04-16 | 2006-04-17 | 삼성전자주식회사 | 다층 박막 구조를 가진 ｄｎａ 칩 |
| WO2004094986A2 (en) * | 2003-04-16 | 2004-11-04 | Handylab, Inc. | System and method for electrochemical detection of biological compounds |
| US20050170367A1 (en) * | 2003-06-10 | 2005-08-04 | Quake Stephen R. | Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids |
| US7572581B2 (en) * | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| WO2005054441A2 (en) * | 2003-12-01 | 2005-06-16 | California Institute Of Technology | Device for immobilizing chemical and biomedical species and methods of using same |
| EP1716254B1 (en) | 2004-02-19 | 2010-04-07 | Helicos Biosciences Corporation | Methods for analyzing polynucleotide sequences |
| US20060046258A1 (en) * | 2004-02-27 | 2006-03-02 | Lapidus Stanley N | Applications of single molecule sequencing |
| US20050239085A1 (en) * | 2004-04-23 | 2005-10-27 | Buzby Philip R | Methods for nucleic acid sequence determination |
| US7476734B2 (en) * | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| JP2008512084A (ja) * | 2004-05-25 | 2008-04-24 | ヘリコス バイオサイエンシーズ コーポレイション | 核酸の配列決定のための方法およびデバイス |
| US20070117104A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| US20070117103A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| US20060024678A1 (en) * | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
| US20060118754A1 (en) * | 2004-12-08 | 2006-06-08 | Lapen Daniel C | Stabilizing a polyelectrolyte multilayer |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US20060172328A1 (en) * | 2005-01-05 | 2006-08-03 | Buzby Philip R | Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction |
| US7482120B2 (en) * | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US7524831B2 (en) * | 2005-03-02 | 2009-04-28 | Schering Corporation | Treatments for Flaviviridae virus infection |
| US20060263790A1 (en) * | 2005-05-20 | 2006-11-23 | Timothy Harris | Methods for improving fidelity in a nucleic acid synthesis reaction |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| GB0518867D0 (en) * | 2005-09-15 | 2005-10-26 | Secretary Trade Ind Brit | Microscopy tip |
| US20070117102A1 (en) * | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
| US20070128610A1 (en) * | 2005-12-02 | 2007-06-07 | Buzby Philip R | Sample preparation method and apparatus for nucleic acid sequencing |
| EP1963530B1 (en) * | 2005-12-22 | 2011-07-27 | Pacific Biosciences of California, Inc. | Active surface coupled polymerases |
| JP2009521227A (ja) * | 2005-12-22 | 2009-06-04 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | ヌクレオチドアナログ組込み用ポリメラーゼ |
| EP1963501A4 (en) * | 2005-12-22 | 2009-04-22 | Pacific Biosciences California | PROTEIN ENGINEERING STRATEGIES FOR OPTIMIZING THE ACTIVITY OF PROTEINS FIXED TO SURFACES |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| EP2089517A4 (en) * | 2006-10-23 | 2010-10-20 | Pacific Biosciences California | POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING |
| US20100081927A1 (en) * | 2008-09-29 | 2010-04-01 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Histological facilitation systems and methods |
| US20100081916A1 (en) * | 2008-09-29 | 2010-04-01 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware. | Histological facilitation systems and methods |
| US20100081928A1 (en) * | 2008-09-29 | 2010-04-01 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Histological Facilitation systems and methods |
| US20100081924A1 (en) * | 2008-09-29 | 2010-04-01 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Histological facilitation systems and methods |
| US20100081915A1 (en) * | 2008-09-29 | 2010-04-01 | Searete Llc, Alimited Liability Corporation Of The State Of Delaware | Histological facilitation systems and methods |
| US20100081926A1 (en) * | 2008-09-29 | 2010-04-01 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Histological facilitation systems and methods |
| FR2940125B1 (fr) * | 2008-12-23 | 2013-03-22 | Isp Investments Inc | Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase |
| FR2940291B1 (fr) | 2008-12-23 | 2012-12-21 | Isp Investments Inc | Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant |
| FR2944445B1 (fr) | 2009-04-15 | 2013-08-16 | Isp Investments Inc | Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant |
| FR2944526B1 (fr) | 2009-04-15 | 2013-05-10 | Isp Investments Inc | Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere |
| US8933036B2 (en) | 2009-04-15 | 2015-01-13 | Isp Investments Inc. | Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair |
| CA2802239C (en) | 2010-06-18 | 2016-08-23 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
| EP2582808B1 (en) | 2010-06-18 | 2015-04-22 | Roche Diagniostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| US8715991B2 (en) | 2010-06-18 | 2014-05-06 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3'-mismatch discrimination |
| JP5841136B2 (ja) | 2010-06-18 | 2016-01-13 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| JP5876477B2 (ja) | 2010-06-18 | 2016-03-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
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| CN103998603B (zh) | 2011-12-08 | 2016-05-25 | 霍夫曼-拉罗奇有限公司 | 具有改进活性的dna聚合酶 |
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| US5292658A (en) * | 1989-12-29 | 1994-03-08 | University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center | Cloning and expressions of Renilla luciferase |
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1998
- 1998-09-03 CA CA002243985A patent/CA2243985C/en not_active Expired - Lifetime
- 1998-09-03 US US09/146,631 patent/US6346379B1/en not_active Expired - Lifetime
- 1998-09-05 EP EP98116786A patent/EP0902035B1/en not_active Expired - Lifetime
- 1998-09-05 AT AT98116786T patent/ATE530567T1/de not_active IP Right Cessation
- 1998-09-05 ES ES98116786T patent/ES2374910T3/es not_active Expired - Lifetime
- 1998-09-07 HU HU9802024A patent/HUP9802024A3/hu unknown
- 1998-09-07 IL IL12610098A patent/IL126100A/xx not_active IP Right Cessation
- 1998-09-07 TW TW087114826A patent/TW513439B/zh not_active IP Right Cessation
- 1998-09-09 CZ CZ19982874A patent/CZ293217B6/cs not_active IP Right Cessation
- 1998-09-10 NO NO19984157A patent/NO327476B1/no not_active IP Right Cessation
- 1998-09-10 AU AU84161/98A patent/AU741366B2/en not_active Ceased
- 1998-09-10 BR BRPI9803419-7A patent/BR9803419B1/pt not_active IP Right Cessation
- 1998-09-10 KR KR1019980037299A patent/KR100561122B1/ko not_active Expired - Fee Related
- 1998-09-11 JP JP25841498A patent/JP4417452B2/ja not_active Expired - Lifetime
- 1998-09-11 RU RU98117244A patent/RU2238978C2/ru not_active IP Right Cessation
- 1998-09-11 CN CNB981245269A patent/CN1197962C/zh not_active Expired - Lifetime
- 1998-09-11 PL PL98328525A patent/PL328525A1/xx not_active Application Discontinuation
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2002
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2003
- 2003-01-30 US US10/355,532 patent/US7598032B2/en not_active Expired - Fee Related
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| AU741366B2 (en) | 2001-11-29 |
| HUP9802024A2 (hu) | 1999-05-28 |
| IL126100A (en) | 2005-12-18 |
| BR9803419B1 (pt) | 2010-09-21 |
| BR9803419A (pt) | 2000-02-08 |
| EP0902035A3 (en) | 1999-06-23 |
| US7598032B2 (en) | 2009-10-06 |
| HK1018796A1 (en) | 2000-01-07 |
| RU2238978C2 (ru) | 2004-10-27 |
| EP0902035B1 (en) | 2011-10-26 |
| CA2243985A1 (en) | 1999-03-11 |
| NO984157D0 (no) | 1998-09-10 |
| NO327476B1 (no) | 2009-07-13 |
| US20030152988A1 (en) | 2003-08-14 |
| HUP9802024A3 (en) | 2001-02-28 |
| CA2243985C (en) | 2004-08-10 |
| US6346379B1 (en) | 2002-02-12 |
| CZ287498A3 (cs) | 1999-03-17 |
| US20020142333A1 (en) | 2002-10-03 |
| JP4417452B2 (ja) | 2010-02-17 |
| CN1218832A (zh) | 1999-06-09 |
| ATE530567T1 (de) | 2011-11-15 |
| KR19990029676A (ko) | 1999-04-26 |
| IL126100A0 (en) | 1999-05-09 |
| PL328525A1 (en) | 1999-03-15 |
| HU9802024D0 (en) | 1998-12-28 |
| JPH11137284A (ja) | 1999-05-25 |
| KR100561122B1 (ko) | 2006-07-25 |
| EP0902035A2 (en) | 1999-03-17 |
| TW513439B (en) | 2002-12-11 |
| CZ293217B6 (cs) | 2004-03-17 |
| NO984157L (no) | 1999-03-12 |
| AU8416198A (en) | 1999-03-25 |
| CN1197962C (zh) | 2005-04-20 |
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